Abstract Periodontal diseases are highly prevalent globally, and represent a significant public health burden that could affect the quality of life in Latin American and in Caribbean countries and territories. The primary objective is to explore the existing research and epidemiological studies on the burden of periodontal diseases, particularly their impact on oral health-related quality of life (OHRQoL) and associations with systemic health conditions in Latin America and the Caribbean (LAC). An electronic literature search was conducted across multiple databases, including MEDLINE (PubMed), Scopus, LILACS, SciELO, and Web of Science, without publication date or language limitations, up until December 2023. Reviewers independently assessed titles and abstracts based on the eligibility criteria. The search yielded 1195 articles, with 63 meeting the inclusion criteria. The results of epidemiological studies showed that periodontitis is extremely prevalent at 90% in LAC; severe periodontitis can affect nearly 10% of the adult population and that periodontitis is aggravated by smoking, poverty, low education level, and limited access to proper dental care. Periodontitis was consistently associated with worse OHRQoL; and causing pain, and/or triggering psychological discomfort, physical disability, and social disability. Associations were also reported between periodontitis and comorbidities such as diabetes, cardiovascular disease, rheumatoid arthritis, respiratory disease, mental illness, and adverse pregnancy outcomes that are also affecting the quality of life of individuals and their families. This scoping review offers a thorough examination of the burden of periodontal diseases in LAC and highlights the significant public health concern that it represents for the region. globally territories healthrelated related OHRQoL (OHRQoL LAC. . (LAC) databases PubMed, PubMed , (PubMed) Scopus LILACS SciELO Science limitations 2023 criteria 119 articles 6 90 10 smoking poverty level care pain andor discomfort disability diabetes disease arthritis illness families region (LAC (PubMed 202 11 9 1 20 2

Abstract The aim of this review was to update knowledge about the prevalence of periodontitis in Latin America and the Caribbean. A critical review of was performed of all cross-sectional or cohort studies selected, pertaining to the region, and thirty-five studies conducted in 12 countries were selected. The countries with nationally representative studies were Brazil, Chile, Colombia, and Uruguay. The prevalence of periodontal disease or need for periodontal treatment varied between the different studies and countries depending on the age group, the methodology used, and the case definition. The prevalence of severe periodontitis aged between 5.8% and 49.7% in adults. In adolescents, the prevalence of moderate to severe periodontitis was 15.3%. Furthermore, a high prevalence of gingival bleeding in adolescents was reported. When analyzing the studies that used the Community Periodontal Index (CPI), Centers for Diseases Control and American Academy Periodontology (CDC/AAP) case definition, it was observed that as the age of the individuals analyzed increased, the prevalence of periodontal disease also increased. Whereas this rereview revealed that although the number of regional and nationally representative studies that analyzed the prevalence of periodontitis has risen in recent years, their methodological heterogeneity prevents global conclusions to be drawn concerning the region. Therefore, this ratifies the need to generate alliances between countries with the purpose of joining individual efforts to achieve collective goals which, among other objectives, will translate into conducting multicenter studies. These studies would allow description and monitoring of the epidemiological behavior of periodontitis in Latin America and the Caribbean. Caribbean crosssectional cross sectional selected region thirtyfive thirty five 1 Brazil Chile Colombia Uruguay group definition 58 5 8 5.8 497 49 7 49.7 adults 153 15 3 15.3% Furthermore reported CPI, CPI , (CPI) CDC/AAP CDCAAP CDC AAP (CDC/AAP increased years Therefore which objectives 5. 4 49. 15.3 (CPI 15.

Abstract There is scarcity of information on the determinants of periodontitis in Latin America and Caribbean countries. We conducted a comprehensive review of studies examining the association of smoking and diabetes with periodontitis outcomes in this region. We searched for population-based, cross-sectional and prospective cohort studies from Latin America and the Caribbean region that reported on the association between smoking or diabetes and periodontitis. Databases were searched up to October 2023 by two reviewers. Subsequently, two authors independently conducted a rigorous data extraction process, focusing on study characteristics, the definition of exposures, and periodontitis outcomes, including measures of association and main findings. The results revealed a significant association between smoking and periodontitis, with a stronger effect observed in heavy smokers. Conversely, while some studies observed a higher prevalence of periodontitis among diabetic individuals, the association between diabetes and periodontitis was not significant after adjusting for confounding factors. These findings underscore a significant research gap in population-based studies on the effect of smoking and diabetes on periodontitis within Latin American and Caribbean countries, particularly when it comes to cohort studies. Addressing these gaps is crucial for a deeper understanding of these associations, which could lead to more effective prevention and treatment strategies in the region. countries populationbased, populationbased population based, based crosssectional cross sectional 202 reviewers Subsequently process characteristics exposures smokers Conversely individuals factors associations 20 2

Abstract This is a new version of the LAOHA Consensus on Periodontal Disease and its impact on general health in Latin America. Five years after the first version, knowledge has increased, and diagnosis of periodontal disease has evolved. Of especial interest relative to this topic has been the emergence of studies that have used the AAP/EFP Classification of Periodontal and Peri-Implant Diseases and Conditions since it is the core (?)of the art of diagnosis in preventive and therapeutic strategies. To make an accurate and effective diagnosis, knowledge about the health-disease process is fundamental. This article updates and demonstrates challenges in periodontal diagnosis, especially in Latin American countries. Considering that periodontal diagnosis should be based on knowledge of the etiopathogenesis of periodontal diseases, this article points out aspects developed in the last few years and emphasizes the knowledge that has been established, considering diagnosis of periodontal diseases. The evidence available stresses the importance of interviewing the patient, by implementing periodontal charting, and requesting any imaging and other complementary tests necessary. An important observation is that the partial periodontal data recordings used for screening (up to now) are not diagnostic methods and might underestimate disease. Moreover, in this article approaches to other forms of recognizing periodontal diseases are described, which could be used, however, not for the purpose of diagnosis, but for increasing awareness and eventually for referring individuals. In Latin American countries there is a need to increase the awareness of periodontal diseases among both the population and the profession, with particular emphasis on prioritizing correct periodontal diagnosis. In Dental education, strategies need to be established with the purpose of understanding that diagnosis is pivotal to any clinical approach. America increased evolved AAPEFP AAP EFP PeriImplant Peri Implant healthdisease fundamental patient charting necessary up now Moreover described however individuals profession education approach

Abstract Periodontal diseases are closely related to non-communicable diseases, and their prevention depends on their link with healthy lifestyle programs. The purpose of this consensus was to summarize and propose preventive strategies at the community, individual, and research levels in Latin America and the Caribbean. A critical review and search strategy was carried out in Pubmed, LILACS, and SCIELO on three topics: a) Social determinants, risk factors, and behavioral changes related to PD throughout the lives of individuals; b) Impact of mechanical and chemical control of plaque for the prevention of gingivitis; c) Impact on prevention of systemic diseases. Relative to public health policies, no consensus was reported in the region. In some countries, periodontal educational strategies, mainly for pregnant women and for other chronic diseases have been implemented, but their impact on primary and secondary prevention has hardly been evaluated. In recent years, a positive aspect has been the implementation of some public policies, including clinical practice guidelines and care pathways. Based on the latest consensus, multicenter educational research and technological strategies were found in the region, but their effectiveness needs to be evaluated in clinical studies. A barrier to the implementation of preventive strategies has to do with the human factor. Therefore, the training of periodontists to be experts in communication strategies, technologies that allow the empowerment of patients for taking care of their periodontal health are required . Moreover, it is necessary to train professionals from other areas of health, who are more aware of the importance of oral health as a healthy lifestyle. noncommunicable non communicable programs community individual Caribbean Pubmed LILACS topics determinants factors individuals b gingivitis c policies region countries implemented years pathways studies factor Therefore Moreover

Abstract The prevalence of periodontitis in Latin American and Caribbean countries (LACC) drew attention to a significant public health issue exacerbated by socio-economic disparities. This consensus report, based on the European Federation of Periodontology (EFP) S3 level clinical practice guidelines, proposed a multifaceted approach to periodontal health-care. The report highlighted the critical need for holistic, population-wide health policies and underscored the lack of documented community interventions in contemporary literature. The consensus advocated for a patient-centered approach to periodontal care, with non-surgical and surgical interventions, and a long-term commitment to supportive periodontal care (SPC). It emphasized the importance of patient engagement in biofilm control by means of home-care and professional interventions for long-term periodontal health. The report also stressed that subgingival instrumentation benefits even severely compromised teeth, by significantly reducing probing depths and gingival inflammation. Moreover, it emphasized the importance of personalized, long-term SPC for maintaining oral health post-treatment, and pointed out the need to identify factors influencing patient adherence. The aim of this report was to provide actionable guidance for clinicians and policymakers, focused on improving periodontal health outcomes and quality of life in LACC. LACC (LACC socioeconomic socio economic disparities EFP (EFP S guidelines healthcare. healthcare care. health-care holistic populationwide population wide literature patientcentered centered nonsurgical non longterm long term SPC. . (SPC) homecare home teeth inflammation Moreover personalized posttreatment, posttreatment post treatment, treatment post-treatment adherence policymakers (SPC

Abstract This analytical cross-sectional study aimed to analyze the access of patients with special needs (PSN) in Brazilian municipalities to hospital dental care of the Unified Health System (Sistema Único de Saúde - SUS), based on data from the Hospital Information System of the Unified Health System (Sistema de Informações Hospitalares do SUS- SIH/SUS - SIH), from 2010 to 2018. The Kolmogorov-Smirnov normality test was performed; the Poisson regression was used to verify factors associated with the variable total number of hospitalization authorizations with the main procedure of dental treatment for PSN (“Total de Autorizações de Internação Hospitalar” – AIH), the Spearman correlation test with a significance level of 5% was used to characterize the relationships between the Municipal Human Development Index per municipality - (Índice de Desenvolvimento Humano Municipal - HDI) and the Oral Health Coverage in the Family Health Strategy by municipality (Cobertura de saúde bucal na estratégia saúde da família por município - SBSF Coverage), and the relationship of the AIH with SBSF Coverage. A total of 127,691 procedures were performed, of which 71,517 (56%) were clinical procedures, such as restorations, endodontic treatments, supra and subgingival scaling, among others. Municipalities in the Midwest (PR=5.117) and Southeast (RP = 4.443) regions had more precedures than the others. A weak correlation was found between AIH and SBSF Coverage (r = -0.2, p < 0.001) and HDI and SBSF Coverage (r = -0.074, p < 0.001). Population size, region, health coverage, oral hygiene, and number of dentists in hospitals affected the availability of dental procedures in PSN. crosssectional cross sectional (PSN Sistema SUS, SUS , SUS) SIHSUS SIH SIH, SIH) 201 2018 KolmogorovSmirnov Kolmogorov Smirnov performed Total Hospitalar AIH, AIH) 5 Índice Cobertura Coverage, Coverage) 127691 127 691 127,69 71517 71 517 71,51 56% 56 (56% restorations treatments scaling others PR=5.117 PR5117 PR 117 (PR=5.117 RP 4.443 4443 4 443 r 0.2, 02 0 2 -0.2 0.001 0001 001 0.074, 0074 074 -0.074 0.001. . size region coverage hygiene 20 12769 12 69 127,6 7151 7 51 71,5 (56 PR=5.11 PR511 11 (PR=5.11 4.44 444 44 0.2 -0. 0.00 000 00 0.074 007 07 -0.07 1276 1 6 127, 715 71, (5 PR=5.1 PR51 (PR=5.1 4.4 0. -0 0.0 0.07 -0.0 ( PR=5. PR5 (PR=5. 4. PR=5 (PR=5 PR= (PR= (PR

Abstract The aim of this cross-sectional study was to investigate the associations between oral health-related quality of life (OHRQoL) and socioeconomic and demographic variables, suicidal ideation, self-perception of oral health, and experiences of dental care in the Brazilian adult LGBTIQ+ population. A sample of 464 participants completed self-administered online questionnaires and provided information for OHRQoL assessment, using the OHIP-14 instrument at three hierarchical levels of explanatory variables: LGBTIQ+ identities; socioeconomic and demographic data and existential suffering; and self-perception of oral health and experience of dental care. The collected data were fitted to hierarchical multiple logistic regression models, in which the associations between each independent variable with the OHIP-14 prevalence outcome were analyzed. The OHIP-14-prevalence index showed that 33.2% of the participants answered ‘frequently’ or ‘always’, and the highest frequencies were obtained for the psychological discomfort (27.8%), psychological disability (18.3%), and physical pain (17.5%) domains. According to the adjusted final model, LGBTIQ+ individuals who were more likely to have their OHRQoL affected were those who were indifferent (OR=3.21; 95% CI: 1.26-8.20), dissatisfied (OR=10.45; 95% CI: 3.86-28.26), or very dissatisfied (OR=53.93; 95% CI: 12.12-239.93) with their oral health status, and also those who had or have difficulty accessing dental treatment (OR=2.06; 95% CI: 1.24-3.41) (p<0.05). It may be concluded that the OHRQoL of the investigated Brazilian LGBTIQ+ population showed associations with individual aspects and with access to dental services. crosssectional cross sectional healthrelated related (OHRQoL variables ideation selfperception self perception LGBTIQ 46 selfadministered administered assessment OHIP14 OHIP 14 OHIP-1 identities suffering models analyzed OHIP14prevalence OHIPprevalence 332 33 2 33.2 ‘frequently frequently ‘always, always ‘always , ‘always’ 27.8%, 278 27.8% 27 8 (27.8%) 18.3%, 183 18.3% 18 3 (18.3%) 17.5% 175 17 5 (17.5% domains model OR=3.21 OR321 OR 21 (OR=3.21 95 CI 1.268.20, 126820 1.26 8.20 1 26 20 1.26-8.20) OR=10.45 OR1045 10 45 (OR=10.45 3.8628.26, 3862826 3.86 28.26 86 28 3.86-28.26) OR=53.93 OR5393 53 93 (OR=53.93 12.12239.93 121223993 12.12 239.93 12 239 12.12-239.93 status OR=2.06 OR206 06 (OR=2.06 1.243.41 124341 1.24 3.41 24 41 1.24-3.41 p<0.05. p005 p p<0.05 . 0 05 (p<0.05) services 4 OHIP1 OHIP- 33. 27.8 (27.8% 18.3 (18.3% 17.5 (17.5 OR=3.2 OR32 (OR=3.2 9 268 1.268.20 12682 126 1.2 820 8.2 1.26-8.20 OR=10.4 OR104 (OR=10.4 8628 3.8628.26 386282 386 3.8 2826 28.2 3.86-28.26 OR=53.9 OR539 (OR=53.9 12239 12.12239.9 12122399 1212 12.1 23993 239.9 23 12.12-239.9 OR=2.0 OR20 (OR=2.0 243 1.243.4 12434 124 341 3.4 1.24-3.4 p00 p<0.0 (p<0.05 27. (27.8 18. (18.3 17. (17. OR=3. OR3 (OR=3. 1.268.2 1268 1. 82 8. 1.26-8.2 OR=10. OR10 (OR=10. 862 3.8628.2 38628 38 3. 282 28. 3.86-28.2 OR=53. OR53 (OR=53. 1223 12.12239. 1212239 121 12. 2399 239. 12.12-239. OR=2. OR2 (OR=2. 1.243. 1243 34 1.24-3. p0 p<0. (p<0.0 (27. (18. (17 OR=3 (OR=3 1.268. 1.26-8. OR=10 OR1 (OR=10 3.8628. 3862 3.86-28. OR=53 OR5 (OR=53 122 12.12239 121223 12.12-239 OR=2 (OR=2 1.243 1.24-3 p<0 (p<0. (27 (18 (1 OR= (OR= 1.268 1.26-8 OR=1 (OR=1 3.8628 3.86-28 OR=5 (OR=5 12.1223 12122 12.12-23 1.24- p< (p<0 (2 ( (OR 1.26- 3.862 3.86-2 12.122 12.12-2 (p< 3.86- 12.12- (p

Abstract This study aimed to evaluate in vitro the effect protocols and anticaries agents containing casein amorphous calcium fluoride phosphopeptide-phosphate (CPP-ACPF, MI Paste Plus), sodium trimetaphosphate (TMP) and fluoride (F), in remineralization of caries lesions. Bovine enamel blocks with initial caries lesions were divided into groups (n = 12): 1) Toothpaste without F-TMP-MI Plus (Placebo); 2) Toothpaste 1100 ppm F (1100F), 3) 1100F + MI Paste Plus (1100F-MI Paste Plus), 4) Toothpaste with 1100F + Neutral gel with 4,500 ppm F + 5%TMP (1100F + Gel TMP) and 5) Toothpaste with 1100F + Neutral gel with 9,000 ppm F (1100F + Gel F). For the 4 and 5 groups the gel was applied only once for 1 minute, initially to the study. For the 3 group, after treatment with 1100F, MI Paste Plus was applied 2x/day for 3 minute. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile and depth of the subsuperficial lesion (PLM); concentrations of F, calcium (Ca) and phosphorus (P) in enamel was determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p < 0.001). Treatment with 1100F alone led to ~ 28% higher remineralization when compared to treatment with 1100F associated with MI Paste Plus (p < 0.001). The 1100F and 1100F + Gel F groups showed similar values for %SHR (p = 0.150). 1100F + Gel TMP treatment also remineralized the enamel surface by ~ 30% and 20% when compared to the 1100F + Gel F and 1100F groups (p < 0.001). The lower lesion depth (ΔKHN) was observed for the 1100F + Gel TMP group (p < 0.001), where it was 54% and 44% lower in comparison to the 1100F and 1100F + Gel F groups (p < 0.001). Polarized light microscopy photomicrographs showed subsurface lesions in all groups, but these lesions were present to a lower extent in the 1100F + Gel TMP group (p < 0.001). Treatment with 1100F + Gel TMP promoted an increase in the concentration of Ca in the enamel by ~ 57% and ~ 26% when compared to the 1100F and 1100F + MI Paste Plus groups (p < 0.001), respectively. There were no significant differences between the 1100F, 1100F + MI Paste Plus and 1100F + Gel F groups (p > 0.001). Similar values of P in the enamel were observed in the 1100F, 1100F + MI Paste Plus and 1100F + Gel F groups (p > 0.001), except for the 1100F + Gel TMP group, which presented a high concentration (p < 0.001). We conclude that the 1100F+TMP gel treatment/protocol led to a significant increased remineralization when compared to the other treatments/protocols and may be a promising strategy for patients with early caries lesions. phosphopeptidephosphate phosphopeptide phosphate CPPACPF, CPPACPF CPP ACPF, ACPF (CPP-ACPF Plus, , Plus) (TMP (F) n 12 12) FTMPMI Placebo (Placebo) 2 110 (1100F) 1100FMI FMI 4500 500 4,50 5TMP 9000 9 000 9,00 F. . F) minute 2xday xday 2x day x cycling SHR (%SHR) ΔKHN PLM (PLM) (Ca (P determined 1criterion criterion (1-criterion StudentNewmanKeuls Student Newman Keuls p 0.001. 0001 0.001 0 001 0.001) 28 0.150. 0150 0.150 150 0.150) 30 20 (ΔKHN 0.001, 54 44 57 26 respectively 1100FTMP FTMP treatmentprotocol protocol treatmentsprotocols treatments (F (Placebo 11 450 50 4,5 900 00 9,0 (%SHR (PLM 0.00 015 0.15 15 45 4, 90 9, 0.0 01 0.1 0.

Abstract Peri-implant diseases, including peri-implant mucositis (PIM) and peri-implantitis, are a chronic inflammatory disorder triggered by bacterial biofilm in susceptible hosts. Potential risk factors for peri-implant diseases include smoking, dental plaque accumulation, poor oral hygiene, genetics, and absence of peri-implant keratinized mucosa. This cohort study aimed to evaluate the influence of patient-, implant-, and prosthetic-related factors on PIM and peri-implant bone loss (PBL) around dental implants after 1 year of loading. A total of 54 subjects (22 males and 32 females) were included in the study. Peri-implant clinical parameters were assessed and standardized periapical radiographs of each dental implant were obtained 15 days after the definitive prosthesis installation (baseline) and at 3, 6, and 12 months of follow-up. A total of 173 implants were evaluated. PIM affected 44.8% of the implants and no significant association was found between the investigated parameters and PIM incidence, except for type of implant connection. A significantly higher incidence of PIM (80.0%) was observed for implants with internal hexagon connection type after 1 year of follow-up (p = 0.015). Moreover, a mean PBL of 0.35 ± 1.89 mm was observed and no dental implant was affected by peri-implantitis after 1 year of function. No specific influence of patient, implant, or prosthetic factors on PBL was observed. No association was found between the occurrence of PIM/PBL and the patient-, implant-, and prosthetic-related factors investigated in this cohort study, except for the type of dental-implant connection. Periimplant Peri periimplant peri (PIM periimplantitis, periimplantitis implantitis, implantitis hosts smoking accumulation hygiene genetics mucosa patient , patient- implant- prostheticrelated related (PBL loading 5 22 (2 3 females baseline (baseline 6 followup. followup follow up. up 17 evaluated 448 44 8 44.8 80.0% 800 80 0 (80.0% p 0.015. 0015 0.015 . 015 0.015) Moreover 035 35 0.3 189 89 1.8 function PIMPBL dentalimplant 2 ( 4 44. 80.0 (80.0 001 0.01 01 03 0. 18 1. 80. (80. 00 0.0 (80 (8

Abstract The aim of this study was to identify and analyze the characteristics of the 100 most cited articles about dental sealants (DS) in dentistry. In September 2023, a search was performed in the Web of Science Core Collection (WoS-CC) database. The following information was extracted from each article: number and density of citations, year of publication, authorship, journal, impact factor, keywords, study design, theme, continent, country, and institution. The citations of the WoS-CC were compared with those of the Scopus and Google Scholar databases. The VOSviewer software was used to generate collaborative networks. The number of citations ranged from 33 to 205. The articles were published between 1961 and 2016. Buonocore MG (7%) was the most prominent author among the most cited. The Journal of the American Dental Association was the most frequent journal (25%) and Journal of Dental Research (7.6) had the highest impact factor. Most studies had interventional (41%) and laboratory (31%) designs, mainly addressing DS effectiveness in the prevention and control of dental caries (86%). There was a predominance of publications from North America (46%) and the USA was the country with the highest number of articles (44%). The most frequent institutions were the Centers for Disease Control and Prevention (USA) and the University of Rochester (USA) (6% each). “Retention” was the most frequent keyword. In conclusion, the 100 most cited articles were mostly interventional and laboratory studies, addressing the retention and efficacy of DS. Most of the articles were concentrated in North America and Europe, demonstrating a little collaboration from other continents. 10 (DS dentistry 2023 WoSCC WoS CC (WoS-CC database article publication authorship factor keywords design theme continent institution databases networks 3 205 196 2016 7% 7 (7% 25% 25 (25% 7.6 76 6 (7.6 41% 41 (41% 31% 31 (31% designs 86%. 86 86% . (86%) 46% 46 (46% 44%. 44 44% (44%) (USA 6% (6 each. each) Retention “Retention keyword conclusion Europe continents 1 202 20 19 201 (7 2 (25 7. (7. 4 (41 (31 8 (86% (46 (44% ( (2 (4 (3 (86 (44 (8

Abstract Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) can differentiate into osteoblasts, indicating that both are potential candidates for bone tissue engineering. Osteogenesis is influenced by many environmental factors, one of which is lipopolysaccharide (LPS). LPS-induced NF-κB activity affects the osteogenic potencies of different types of MSCs differently. This study evaluated the effect of LPS-induced NF-κB activity and its inhibition in DPSCs and PDLSCs. DPSCs and PDLSCs were cultured in an osteogenic medium, pretreated with/without NF-κB inhibitor Bay 11-7082, and treated with/without LPS. Alizarin red staining was performed to assess bone nodule formation, which was observed under an inverted light microscope. NF-κB and alkaline phosphatase (ALP) activities were measured to examine the effect of Bay 11-7082 pretreatment and LPS supplementation on osteogenic differentiation of DPSCs and PDLSCs. LPS significantly induced NF-κB activity (p = 0.000) and reduced ALP activity (p = 0.000), which inhibited bone nodule formation in DPSCs and PDLSCs. Bay 11-7082 inhibited LPS-induced NF-κB activity, and partially maintained ALP activity and osteogenic potency of LPS-supplemented DPSCs and PDLSCs. Thus, inhibition of LPS-induced NF-κB activity can maintain the osteogenic potency of DPSCs and PDLSCs. (DPSCs (PDLSCs osteoblasts engineering factors . (LPS) LPSinduced NFκB NF κB differently medium withwithout with without 117082, 117082 11 7082, 7082 microscope (ALP 11-708 p 0.000 0000 0 000 0.000, , LPSsupplemented supplemented Thus (LPS 11708 1 708 11-70 0.00 00 1170 70 11-7 0.0 117 7 11- 0.

Abstract This study assessed the reliability of a color measurement method using images obtained from a charge-coupled device (CCD) camera and a stereoscopic loupe. Disc-shaped specimens were created using the composite Filtek Z350 XT (shades DA1, DA2, DA3, and DA4) (n = 3). CIELAB color coordinates of the specimens were measured using the spectrophotometer SP60 over white and black backgrounds. Images of the same specimens were taken using a CCD camera attached to a stereoscopic loupe. The color of the image was measured (red–green–blue [RGB]) using an image processing software and converted to CIELAB coordinates. For each color coordinate, data from images were adjusted using linear regressions predicting those values from SP60. The whiteness index for dentistry (WID) and translucency parameter (TP00) of the specimens as well as the color differences (ΔE00) among pairwise shades were calculated. Data were analyzed via repeated-measures analysis of variance and Tukey’s post hoc test (α = 0.05). Images obtained using the loupe tended to be darker and redder than the actual color. Data adjustment resulted in similar WID, ΔE00, and TP00 values to those observed for the spectrophotometer. Differences were observed only for the WID of shade DA3 and ΔE00 for comparing DA1 and DA3 over the black background. However, these differences were not clinically relevant. The use of adjusted data from images taken using a stereoscopic loupe is considered a feasible method for color measurement. chargecoupled charge coupled (CCD Discshaped Disc shaped Z Z35 DA DA2 DA4 n 3. 3 . 3) SP SP6 backgrounds red–green–blue redgreenblue red green blue RGB [RGB] coordinate (WID TP (TP00 ΔE (ΔE00 calculated repeatedmeasures repeated measures Tukeys Tukey s α 0.05. 005 0.05 0 05 0.05) TP0 ΔE0 background However relevant Z3 [RGB (TP0 (ΔE0 00 0.0 (TP (ΔE 0.

Abstract This cross-sectional study evaluated the association between salivary immunoglobulins, plaque index, and gingival index in Brazilian children with and without type 1 diabetes mellitus (DM1). The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) checklist for the reporting of observational studies was followed. The DM1 group had 38 children, and an equal number of volunteers matched by sex and age were recruited as controls. Clinical examination was performed for plaque index and gingival index determination. Non-stimulated whole saliva was collected. Concentrations of IgA, IgG, and IgM were determined by ELISA test. Data were tested by the Kolmogorov-Smirnov, Mann-Whitney, and Spearman tests and a multiple linear regression model (p<0.05) was performed. Gingival index was higher in the Control (DM1: 0.16±0.17; Control: 0.24±0.23, p=0.040). In DM1, there was a correlation between IgA and age (rho=0.371, p=0.024), IgM and IgG (rho=0.459, p=0.007), and IgM and gingival index (rho=0.394, p=0.014). In DM1, multiple linear regression showed that age (p=0.041; β=0.363), gingival index (p=0.041; β=0.398), and plaque index (p=0.008; β=-0.506) were good predictors of IgA levels in saliva. Thus, IgA was the only researched immunoglobulin that was directly associated with plaque and gingival indices in Brazilian children with DM1, but not in control subjects. crosssectional cross sectional immunoglobulins DM1. DM . (DM1) STROBE (STROBE followed 3 controls determination Nonstimulated Non stimulated collected test KolmogorovSmirnov, KolmogorovSmirnov Kolmogorov Smirnov, Smirnov Kolmogorov-Smirnov MannWhitney, MannWhitney Mann Whitney, Whitney Mann-Whitney p<0.05 p005 p 0 05 (p<0.05 (DM1 0.16±0.17 016017 16 17 024023 24 23 0.24±0.23 p=0.040. p0040 p=0.040 040 p=0.040) rho=0.371, rho0371 rho 371 (rho=0.371 p=0.024, p0024 p=0.024 , 024 p=0.024) rho=0.459, rho0459 459 (rho=0.459 p=0.007, p0007 p=0.007 007 p=0.007) rho=0.394, rho0394 394 (rho=0.394 p=0.014. p0014 p=0.014 014 p=0.014) p=0.041 p0041 041 (p=0.041 β=0.363, β0363 β β=0.363 363 β=0.363) β=0.398, β0398 β=0.398 398 β=0.398) p=0.008 p0008 008 (p=0.008 β=0.506 β0506 β= 0.506 506 β=-0.506 Thus subjects p<0.0 p00 (p<0.0 (DM 0.16±0.1 01601 02402 2 0.24±0.2 p004 p=0.04 04 rho=0.371 rho037 37 (rho=0.37 p002 p=0.02 02 rho=0.459 rho045 45 (rho=0.45 p000 p=0.00 00 rho=0.394 rho039 39 (rho=0.39 p001 p=0.01 01 (p=0.04 β036 β=0.36 36 β039 β=0.39 (p=0.00 β=0.50 β050 0506 0.50 50 β=-0.50 p<0. p0 (p<0. 0.16±0. 0160 0240 0.24±0. p=0.0 rho=0.37 rho03 (rho=0.3 rho=0.45 rho04 4 (rho=0.4 rho=0.39 (p=0.0 β03 β=0.3 β=0.5 β05 050 0.5 5 β=-0.5 p<0 (p<0 0.16±0 016 0.24±0 p=0. rho=0.3 rho0 (rho=0. rho=0.4 (p=0. β0 β=0. 0. β=-0. p< (p< 0.16± 0.24± p=0 rho=0. (rho=0 (p=0 β=0 β=-0 (p 0.16 0.24 p= rho=0 (rho= (p= β=- 0.1 0.2 rho= (rho