Abstract Palmitoleic acid (C16:1n7, POA), a 16-carbon ω-7 monounsaturated functional fatty acid, has been demonstrated a variety of beneficial properties to human health and a wide range of applications in nutrition, medical and chemical industries. Lipozyme® RM IM (Rhizomucor Miehei lipase), a Sn-1,3 specific lipase, was used to catalyze the hydrolysis of seabuckthorn fruit oil and produce monoglycates rich in palmitoleic acid. The fruit oil of seabuckthorn was used as the research object, the dosage of enzyme taking 10% wt.% oil, the influence of water/oil ratio, pH value and reaction temperature to hydrolysis rate were investigated, and then the response surface methodology was applied for optimizing the process conditions. Further, the relationship between the hydrolysis rate and the content of monoacylglycerol was studied under the optimal hydrolysis conditions. The result indicated that the optimal combined condition is as follows: the reaction temperature is 55°C, the pH is 7, the water-oil quality ratio is 1:1. The hydrolysis rate achieved 79.59% within 12 h in the condition above. Under the optimal conditions, the hydrolysis rate was positively correlated with the content of monoester, and the correlation coefficient was 0.999 within the first 10 h of the reaction. The hydrolysis rate was 66.76% at 10 h, and the content of monoglyceride reached the highest of 61.11%. Hereafter, the hydrolysis of monoglyceride began if the reaction continued. The content of palmitoleic acid increased from 27.11% to 40.84%, and that of linoleic acid increased from 4.51% to 33.34%, so the total amount of the two functional fatty acids reached 74.18%. Enzymatic hydrolysis can be an effective way to improve the function of seabuckthorn fruit oil.

Abstract The aim of this study was to investigate the anticancer effect of Oenanthe stolonifera D.C extract (OJE) on Hep3B, a human liver cancer cell, and identify the anticancer mechanisms. When treated with 1.0 mg/mL of OJE in HEK-293 and HEP3B to test the anticancer effect based on cell viability and mobility, the extract showed no growth inhibitory effect on HEK-293, while Hep3B's cell viability and mobility significantly decreased to 62.5% and 48.6%, respectively. The RNA expression levels of AMP-activated protein kinase (AMPK), protein-53 (p53), and cyclooxygenase-2 (COX-2), which are key genes of carcinogenesis, were examined to investigate the mechanism of the anticancer effect. OJE downregulated COX-2 and increased the expression of cancer suppressors AMPK and p53 in a concentration-dependent manner. HPLC analysis was conducted to quantify secondary metabolites in OJE, and 0.50 mg/g DM of chlorogenic acid was identified as the main substance. Therefore, Oenanthe stolonifera D.C containing bioactive substances is valuable as a natural source for anticancer agent in the food and pharmaceutical industries.

Abstract Fish hydrolysates have become one of the most remarkable sources of bioactive peptides. However, the processing conditions for incorporating hydrolysates into food matrices can affect their bioactive performance. The effect of temperature and pH on the radical scavenging activity of tilapia hydrolysate was determined in the wet hydrolysate. Also, a central composite design was used to study the effect of the drying conditions on moisture, drying ratio, productivity, drying rate, and antioxidant activity in the tilapia hydrolysate. The results showed that the hydrolysate has high activity at acidic and neutral pH; but at pH 10, the activity decreases significantly. In the spray-drying process, the antioxidant activity was higher at 115 °C. Moreover, inlet air temperature and feed flow had a statistically significant effect (p < 0.05) on response variables. High inlet air temperature and fast feed flow decrease the moisture of the powder hydrolysate and increase the drying rate and antioxidant activity. Scanning electron microscopy showed liquid bridges between particles with irregular concavities or pores on the surface and the presence of particle agglomerations due to the hygroscopicity of the hydrolysate.

Abstract Food security is among the most important aspects of our physical health; it is necessary to live a full and peaceful life. Food security is a wide term that encompasses more than crop production since it necessitates taking into consideration geographical and temporal variability, along with economic and physical availability. Drought and salt stress are two major variables that restrict agricultural yield. These pressures are expected to worsen as a result of climate change, posing even greater threats to global food security. Earth remote sensing (ERS) using unmanned aerial vehicles is operational monitoring of the state of fields, the quality of processing of fallow lands; provides control of the degree of weediness of fields during the period of spring fieldwork, which makes it possible to rationally distribute agricultural machinery for closing moisture, intermediate treatments and pre-sowing chemical treatments sowing quality control; monitoring the vegetation index makes it possible to construct effective schemes for plant feeding, as well as effectively distribute agricultural machinery during the harvesting period, depending on the degree of crop maturation. The yield mapping system allows evaluating the effectiveness of precision agriculture techniques, making it possible to accumulate data on productivity zones, considering the actual yield.

Abstract The purpose of this study was to determine the effect of Trehalose and Telang-Pea (Clitoris ternatea L.) which are used as fillers for Telang-Yellow milk powder. This study used a randomized block design, 3x3 factorial, and 2 replications. Trehalose concentrations (10%, 15% and 20%) and t butterfly pea flower concentrations (0.5%, 1% and 1.5%) were research factors. The results of this study included water content, antioxidant activity, viscosity, yield, solubility, dissolution time, hygroscopicity, color intensity, and preference for taste, color, aroma and after taste. The results showed that water content, hygroscopicity, dissolution time, solubility, yield, pH value, color, and hedonic assessment of taste, color, aroma, and aftertaste were affected by trehalose and eggplant concentrations. The best organoleptic results were obtained with t1b2 samples (0.5% trehalose and 15% Butterfly Pea). The best treatment was the t3b3 treatment with the results of the analysis of water content 2.86%, hygroscopicity 7.15%, amendment 20.00%, soluble time 29.13%, solubility 83.98%, pH 6.72, antioxidant activity 557.77 ppm, color intensity *L 48.01, *a 4.73 and *b 6.10.

Abstract This paper evaluates the results of extracting bioactive compounds using modern maceration technology, which transforms food products into a liquid extract, based on the example of unripe grapes and its by-product called verjuice. During the extraction process, the indicators of niacin and pyridoxine remained stable, while pantothenic acid and choline increased significantly. During the extraction, the extraction system produced 712 kg of liquid extract, which was characterized by a high content of phenolic compounds and water-soluble vitamins, as well as a high antioxidant activity compared to the measurement obtained for green juice from unripe grapes. Since the estimated liquid extract yield from 950 kg of crushed immature grapes is 847 kg, the actual liquid extract yield was about 80%. The advantages of the maceration extraction method included avoiding the use of solvents or preservatives, low temperature treatment and little oxidative damage. Besides, it enabled enhancing the extraction yield due to introduction of certain post-extraction steps.

Abstract The woody oil industry generates a large amount of seed shell waste during its production. Therefore, this paper employs a central composite design (CCD) methodology to optimize the ultrasonic-assisted extraction process, thus utilizing the byproduct. The infrared spectrum and thermogravimetric analysis is adopted to explore the chemical structure and thermal stability of tannin. Besides, the tannin resin (TF) is prepared to elaborate its adsorbing performance for dyes in water. The results verify the role of ultrasonic-assisted extraction method in increasing yield rate. The optimal conditions are as follows: 60% ethanol as extraction solvent, the solid-liquid ratio of 1:25 (g/mL), ultrasonic power of 160 W, and extracted at 80oC for 60min. The yield rate of tannin under such conditions stands at 32.04% (w/w). Temperature is vital for the extraction rate. Acer truncatum tannin as a condensed tannin, performs favorable thermal stability, and TF effectively absorbs cationic dye in water. The maximum adsorption capacity of methylene blue peaks at 176.13 mg/g. Acer truncatum shell serves as a resource of condensed tannin, and Acer truncatum tannin resin can be potentially developed into a new biomass adsorbent.

Abstract Heigen is the root and rhizomes of Duhaldea Nervosa Anderberg., a traditional medicine food homology herb used to treat rheumatoid arthritis in China,but the potential active components and the underlying mechanism have not been clarified. In this study, the chemical compounds and the components absorbed into the blood from Heigen were assessed using ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry. 72 chemical constituents were identified in Heigen, 13 blood prototypical constituents, and 9 metabolites were found in serum samples after intragastric administration of Heigen extracts to rats. Then, using network pharmacology and molecule docking, 10 compounds were assumed to be potentially key active compounds against RA, which may be via the multi-component, multi-target and multi-pathway interaction mechanism of Heigen in the treatment of RA, and also provides a pharmacological basis for the treatment of RA.

Abstract Dried radish is a kind of pickled vegetable product with a unique taste made from radish via traditional solid-state spontaneous fermentation. In this study, the effects of variety on physicochemical indexes, texture properties, and taste substances of spontaneous fermented dried radish were investigated. As a result, processing and fermentation had similar trends for all radish varieties. The pH value decreased slightly, soluble solid increased with the decrease of moisture content, the hardness and chewiness decreased, while the content of organic acids and free amino acids increased. Furthermore, the variety of raw material is the key to affecting product quality and taste. The dried radish processed by the variety P21-25 presents the best texture, the highest content of sweet amino acids, the most abundant component of organic acids, while having the lowest salinity. These results indicated that radish varieties could significantly influence the quality and taste of dried radishes under the same processing and fermentation conditions. These findings reveal the importance of variety selection for the quality and taste of spontaneous fermented dried radish, and provide theoretical support for the processing and application of green radish varieties.

Abstract Faced with changes in eating habits, consumers currently seek to enjoy moderate consumption of healthy food products that results in sensory pleasure. Here, our study aimed to evaluate the biochemical and physicochemical properties and sensory profile of craft beers produced in the Brazilian Midwest region. Our evaluation of the beverages revealed different physicochemical characteristics such as alcohol content, soluble solids, titratable acidity in citric acid, and dry extract percentage, with values ranging between 4.06-6.13%, 5.13-10.98 °Brix, 1.71-3.45%, and 3.38-7.99%, respectively. Moreover, the average density of the beverages was 0.9906 g/cm3. In addition, the antioxidant activity and total phenolic of the beverages ranged between 14.28-20.46 mMol of Trolox/100 mL and 74.84-108.45 mg/100 g, respectively. Phenolic compounds that are essential in human nutrition, such as epicatechin, catechin, gallic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin, and kaempferol, were identified in the beverages. Regarding the sensory profile of the craft beers, the beers had an average acceptability index of 75.11% for appearance, 70.24% for aroma, 60.43% for flavor, and 73.53% for texture. Our study demonstrated a correlation between chemical composition and acceptability index of beer, indicating a preference for lighter colored, fuller, and less acidic beers.

Abstract Powders were prepared from fresh Phyllostachys pubescens bamboo shoots (P. pubescens), Dendrocalamopsis oldhami bamboo shoots (D.oldhami) and Dendrocalamus latiflorus bamboo shoots (D.latiflorus), and their physical, chemical, adsorption and powder properties were determined. All three of these powders were high in dietary fiber and protein, with P. pubescens powder containing the highest concentration of dietary fiber (50.34 ± 0.65 g∙100 g-1). The water holding, oil holding, and swelling capacities of this powder were all higher than those of the other two kinds of powders. The P. pubescens powder also showed the highest capacity to adsorb glucose, cholesterol and nitrite. In addition, the measured repose and slip angles of this powder were lower than those of the other two powders, indicative of it having good fluidity. And the bulk density and tap density of this powder were significantly higher than those of the others as well, indicative of its superior filling property.

Abstract Syringodium isoetifolium was seagrass widely grown on the coast of Indonesia, and was the phytochemical source. This research was conducted to maximalize the extraction of their phytochemicals and bioactive compounds. Firstly, seagrass powder was extracted with different solvent polarities i.e. water, 50% ethanol, and 100% ethanol. Secondly, extraction was continued using different extraction techniques i.e. microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE-Bath system and UAE-Probe system), and conventional with the best solvent. Phytochemicals, bioactive compounds, antioxidant activity, and scanning electron microscope (SEM) were analyzed on the seagrass extracts. As result, the total phytochemical kind (i.e. terpene, polyphenols, alkaloid, and amino acid derivative groups) in the most dominant was had by 50% ethanol extract. Accordingly, extraction using the UAE-Probe with 50% ethanol as solvent able to obtain the richest bioactive compound, the most damaged cell microstructure, and the strongest antioxidant activity. Interestingly, quercetin was only detected dominant in UAE-Probe extract. Therefore, UAE-Probe with 50% ethanol solvent was the best method for recovering the valuable components in seagrass.

Abstract This study was undertaken to investigate the potential effects of wheat flour substitution with potato starch modified by heat-moisture treatment (HMTS) and microwave treatment (MWS) on dough texture properties and on the quality of fresh noodles. The research results showed that the substitution of wheat flour with HMTS or MWS altered the texture and tensile properties of dough and cooked fresh noodles. Moreover, the dough tensile properties of resistance to extension and extensibility were extremely significant positive correlated with the cooked fresh noodles tensile properties of tensile strength and elasticity. The optimal cooking time of fresh noodles was significantly decreased (P value < 0.05) with the increase incorporation of HMTS or MWS. The dry matter water absorption rate and loss rate of dry matter significantly increased with the increase of substitution amount of HMTS and MWS. When the incorporation amount of HMTS was less than 30% and the incorporation amount of MWS was less than 20%, the noodles could maintain good organoleptic and cooking quality attributes. More than 30% of HMTS or 20% MWS will deform the noodles and cause breakage. Therefore, the maximum incorporation of HMTS or MWS should be 30%, 20%, respectively.

Abstract Cell apoptosis induction and metastasis suppression in human breast cancer cell, MDA-MB-231, were studied to assess the potential of Cirsium setidens extract (CSE) on breast cancer treatment. Cell viability and metastasis analysis using MDA-MB-231 confirmed CSE significantly inhibited the growth and proliferation of cancer cell without suppressing normal cell growth in the range of 0.25 to 0.5 mg/mL of CSE. To analyze the anticancer effect of CSE, the expressions of major genes related to in the apoptosis, including p53, AMPK-α, Bcl-2, and Bax were analyzed by reverse transcription-polymerase chain reaction. The expressions of AMPK-α, p53, and Bax increased by 45, 43, and 68%, respectively, while the expression of Bcl-2 decreased by 12%, showing that CSE treatment induced apoptosis of MDA-MB-231. The major compound of CSE was identified as rhoifolin, a type of flavone, through analysis using LC-MS/MS. Our findings confirmed that rhoifolin, known to possess a variety of significant biological activities, could be obtained from C. setidens and used as an effective natural compound to suppress breast cancer.

Abstract The purpose of this study is to investigate the effect of bioconverted Oenanthe javanica extract (BOE) using Lactiplantibacillus plantarum SM4 on production of bioactive compounds. Response surface methodology was used to determine the optimal ultrasound-assisted extraction conditions including the extraction time, extraction temperature, and ethanol concentration for the enhancement of bioactive compounds production. The predicted optimum UAE conditions were extraction time of 26.0 min, extraction temperature of 92.8 °C, and 59.9% ethanol, respectively, and 5.14 mg GAE/g DM of TPC and 0.59 mg QE/g DM of TFC were produced under the optimum condition. Then, O. javanica extraction (OJE) has been bioconverted by using L. plantarum SM4 to increase the bioactive compounds. In HPLC-mass spectrometry analyses, higher concentration of p-coumaric acid was identified in BOE than in OJE, and chlorogenic acid in OJE was converted to quinic acid, confirming an increase in bioactive compounds by bioconversion. Thus, we have concluded that optimization of UAE and the BOE is an effective process for enhancement bioactive compounds from O. Javanica and this finding provides a scientific basis of bioactive compounds in natural product can be utilized as food, cosmetics, and pharmaceutical materials from BOE.