Aim To carry out a retrospective analysis of the frequency and severity of clinical signs of radioiodine (131I)-induced damage to the salivary glands in the early and long-term post-radiation periods, and identify risk factors for their occurrence in patients with differentiated thyroid carcinomas. Methods A total of 330 patients underwent thyroidectomy with dissection of lymphatic nodes. One month after surgery, all the patients received radioiodine therapy. The dose and number of courses varied depending on the stage and morphological type of the tumor. In the late post-radiation period, the patients were surveyed with the use of a standard questionnaire, which allowed retrospective assessment of the nature and severity of symptoms of radiation-induced damage, as well as the time of their onset/subsidence. Results Radiation-induced sialoadenitis of the salivary glands was observed in 51.2% of patients treated with 131I. The main symptoms included pain and discomfort in the salivary glands (51.2% of patients), swelling (48.8%), transient or permanent dry mouth (38%), and distortion of taste (38%). There were statistically significant correlations between the presence and severity of the main clinical symptoms of salivary gland irradiation. A significant relationship (r = 0.91, p < 0.001) was found between swelling of the salivary glands and the feeling of pain or discomfort, which was indicative of inflammation and retention of saliva. Conclusion The main factors influencing the formation of chronic radiation-induced sialoadenitis and the severity of the inflammatory process included the tumor stage, the total dose of radiopharmaceuticals, and the duration following radioiodine therapy. 131Iinduced Iinduced 131I induced I longterm long term postradiation post radiation periods carcinomas 33 nodes surgery therapy period questionnaire radiationinduced onsetsubsidence onset subsidence onset/subsidence Radiationinduced Radiation 512 51 2 51.2 (51.2 patients, , patients) 48.8%, 488 48.8% 48 8 (48.8%) 38%, 38 38% (38%) 38%. . irradiation r 091 0 91 0.91 0.001 0001 001 saliva radiopharmaceuticals 3 5 51. (51. 48.8 4 (48.8% (38% 09 9 0.9 0.00 000 00 (51 48. (48.8 (38 0. 0.0 (5 (48. (3 ( (48 (4

Aim This study aimed to perform an in vitro comparative analysis of the antifungal activity of different calcium silicate-based endodontic sealers against three fungal species. Methods The antifungal properties of three calcium silicate-based sealers were tested: Bio-C Sealer, Cambiar a Sealer Plus BC, and MTA-Fillapex. Two commonly used sealers were used as controls: AH Plus and Endomethasone. An agar diffusion test was performed to analyze the antifungal activity of the sealers against Candida albicans, Candida glabrata, Candida tropicalis, and a mixed microbial culture medium. The results were analyzed using ANOVA (p <0.05). Results Endomethasone exhibited the highest inhibition against all strains examined, maintaining a consistent level of inhibition throughout 7 days. MTA-Fillapex demonstrated the best performance among the calcium silicate-based sealers for the three fungal species (p < 0.05), maintaining stable values over the 7 days, surpassing that of Endomethasone. Nevertheless, MTA-Fillapex only exhibited antimicrobial effect against the mixed culture for the first 24 hours, and no antimicrobial activity was observed at 48 hours, being surpassed by all tested sealers (p < 0.05). Conclusion Of all silicate-based sealers tested, only MTA-Fillapex exhibited promising antifungal activity. Nevertheless, care must be taken when extrapolating these results, as MTA-Fillapex exhibited poor antimicrobial activity when tested in mixed microbial cultures. silicatebased silicate based BioC Bio C BC MTAFillapex. MTAFillapex MTA Fillapex. Fillapex controls albicans glabrata tropicalis medium p <0.05. 005 <0.05 . 0 05 <0.05) examined days 0.05, 0.05 , 0.05) Nevertheless 2 hours 4 0.05. cultures 00 <0.0 0.0 <0. 0. <0

Aim The aim of this in vitro study was to compare machine and manual cementation of prosthetic elements by measuring internal and marginal fits. Methods Eighteen anatomic prefabricated abutments were used to manufacture zirconia copings in the Ceramill (n=9) and Lava systems (n=9). The copings were cemented with a fluid consistency addition silicone using a machine (n=18) and manually (n=18) according to the replica technique. They were then cut in the buccal-palatal and mesial-distal directions. The film thickness was photographed using an optical microscope and measured in the internal and marginal regions. The data collected were analyzed by repeated measures ANOVA and Bonferroni’s multiple comparison test (∂=.05). The Bland-Altman test was performed to evaluate the agreement between the methods. Results In the evaluation of the internal and marginal misfits, the mean values observed for the cementation performed with the aid of a machine and manually, were as follows: angular regions, 76.7 µm and 76.2 µm; linear regions, 60.6 µm and 60.7 µm; incisal region, 144.8 µm and 145.2 µm; marginal region, 40.1 µm and 40.2 µm; and overall mean, 80.4 µm and 80.6 µm, respectively. No significant differences were found between the 2 methods, for any of regions and systems (P>.05). The Bland-Altman test showed agreement between the methods (P>.05) and that the limits of agreement found were clinically acceptable. Conclusions Within the limitations of this in vitro study, we can conclude that cementation using manual techniques or mechanical aid produces the same cement films. fits n=9 n9 n 9 (n=9 n=9. . n=18 n18 18 (n=18 technique buccalpalatal buccal palatal mesialdistal mesial distal directions Bonferronis Bonferroni s ∂=.05. 05 ∂=.05 (∂=.05) BlandAltman Bland Altman misfits follows 767 76 7 76. 762 606 60 6 60. 607 region 1448 144 8 144. 1452 145 145. 401 40 1 40. 402 804 80 4 80. 806 respectively P>.05. P05 P P>.05 (P>.05 acceptable films n= (n= n=1 n1 (n=1 0 ∂=.0 (∂=.05 14 P0 P>.0 (P>.0 (n ∂=. (∂=.0 P>. (P>. ∂= (∂=. P> (P> ∂ (∂= (P (∂ (

Aim This study aimed to evaluate the millimeter distances and active tip diameters of different periodontal probes. Methods Two types of periodontal probes were analyzed (North Carolina (15-UNC) and PCP-12). Two manufacturers were selected for each probe type. Digital images of the probes were obtained and the distances were measured using a software program. The diameter of the active tip was measured using a digital caliper. Both variables were measured by two trained and calibrated examiners. The data were analyzed using the Bland-Altman method and two-way ANOVA with Tukey’s post-hoc test. Statistical significance was set at p<0.05. Results A comparison of measurements between the 15-UNC and PCP-12 probes showed a significant difference in all millimeter markings. The 15-UNC probe showed differences between the 3 and 12 mm markings. The PCP-12 probe only showed differences between the marks at the 12 mm mark. The 15-UNC probe had a similar active tip diameter between the two manufacturers. The PCP-12 probe showed a significant difference between the two manufacturers. Both types of probes had similar active tip diameters when compared by the two manufacturers. Conclusion There was no standardization in relation to millimeter marks and tip diameters of the two types of periodontal probes produced by the two different manufacturers. The probe types exhibited little variability. North 15UNC UNC 15 (15-UNC PCP12. PCP12 PCP . PCP-12) type program caliper examiners BlandAltman Bland Altman twoway way Tukeys Tukey s posthoc post hoc test p005 p 0 05 p<0.05 PCP-1 markings 1 mark variability PCP1 p00 p<0.0 PCP- p0 p<0. p<0 p<

Lip necrotizing lesions are painful, complex to heal, and negatively impact the patient’s orofacial condition. The present study reports a clinical case of a necrotizing lesion on the lower lip in a 38-year-old male patient, previously healthy, five days after receiving the first dose of the SARS-COV-2 vaccine, with an initial diagnosis of labial herpes zoster, with secondary infection and unsatisfactory response to clinical treatment. After debridement of the necrotic tissue, followed by a combination of antimicrobial photodynamic therapy (aPDT), photobiomodulation therapy (PBMT) and hydrofiber dressing with silver, the patient evolved with complete healing of the lip lesion and satisfactory restoration of orofacial functions after 7 days. According to the present case report, this combination of phototherapy modalities suggests to be a promising tool for the treatment of necrotizing labial lesions. painful heal patients s condition 38yearold yearold 38 year old healthy SARSCOV2 SARSCOV SARS COV 2 SARS-COV- vaccine zoster tissue aPDT, aPDT , (aPDT) PBMT (PBMT silver report 3 SARS-COV (aPDT

Aim Cyclooxygenase enzymes (COX) catalyze the conversion of arachidonic acid to prostaglandins and thromboxanes during pain and inflammation conditions. These enzymes have also been linked to several other conditions and diseases, and hence, in dentistry, it is crucial to identify the processes that increase the levels of these mediators. This paper aims to describe the significance of COX in dental practice through a narrative review. Methods Articles relating to COX upregulation published in English and Spanish over the last 51 years in databases such as EBSCO, Google Scholar, Science Direct, PubMed, and Web of Science; were analyzed. Results A total of 115 articles demonstrating the relationship between COX upregulation and multiple conditions and diseases of importance in prosthodontics, periodontics, oral pathology, orthodontics, and endodontics were included. Conclusions COX upregulation is related to inflammatory and malignant diseases in oral tissues, such as periodontitis, pulpitis, and oral cancer, nevertheless, its expression is advantageous in other fields of study such as orthodontics. Additionally, is well documented that dental materials provoke an undesired increase in COX expression, which could be a significant factor that directly affects pulpal health. (COX hence dentistry mediators review 5 EBSCO Scholar Direct PubMed analyzed 11 prosthodontics periodontics pathology orthodontics included tissues periodontitis pulpitis cancer nevertheless Additionally health 1

Aim In recent years, great advances have been made in the use of CAD/CAM to prepare fixed restorations. The marginal and internal fit of these restorations is a principal determinant for their clinical success. In addition, the nature of the oral environment affects the mechanical properties of these restorations. Therefore, this study aimed to investigate the effect of aging process under conditions that simulate the oral environment on the marginal adaptation, and, fracture resistance of crowns fabricated from polyether ether ketone (PEEK) using CAD/CAM methods. Methods Twenty identical crown restorations were fabricated by using CAD/CAM methods to mill polyether ether ketone (PEEK) material. These crowns were produced by using a software design of an epoxy resin replica of the prepared maxillary first premolar tooth. All PEEK crowns were cemented and randomly divided into two equal groups (A, B). Each group was divided into subgroups (A1, A2 and B1, B2). Group A1 and A2 were used to measure marginal adaptation and fracture resistance, respectively, before aging, while group (B1 and B2) were measured after aging. The cemented crowns were mounted in resin molds to facilitate the sectioning process. The measurements of the marginal gap were performed after sectioning at four points using a stereomicroscope. The fracture resistance of the crowns was investigated using a universal testing machine. A statistical analysis was performed using the GraphPad Prism® software version and unpaired Student’s t-test. Results The results revealed that aging affected the marginal gap, and the fracture resistance of the PEEK crowns. While aging, negatively affected the conditions under investigation, however the least significant difference of marginal gap was found in the margin region. Conclusions PEEK-CAD/CAM is considered as a good alternative prosthodontic material for fixed prostheses. The CAD/CAM technique used to make PEEK crown restorations in our study offers the advantages of high marginal accuracy and fracture resistance for long-term performance in the oral environment. years CADCAM CAD CAM success addition Therefore (PEEK tooth A, (A B. B . B) A1, (A1 B1 B2. B2 respectively (B stereomicroscope machine Prism Students Student s ttest. ttest t test. test t-test investigation region PEEKCAD/CAM PEEKCADCAM prostheses longterm long term PEEKCAD

Aim To evaluate the surface roughness and color stability of bulk-fill resin composites after simulated toothbrushing with whitening dentifrices. The radioactive/relative dentin abrasion (RDA) and radioactive/relative enamel abrasion (REA) of dentifrices were also assessed. Methods Specimens (n=10) of Tetric N Ceram Bulk Fill (TNCB), Filtek One Bulk Fill (FOB) resin composites, and Z100(Control) were prepared using a cylindrical Teflon matrix. Surface roughness (Ra, μm) was assessed by a roughness meter and the color evaluations (ΔEab, ΔE00, WID) were performed using a digital spectrophotometer based on the CIELAB system. Three measurements were performed per sample, before and after simulated toothbrushing with 3D Oral-B White Perfection (3DW) and Black is White (BW) dentifrices. The abrasivity (REA and RDA values) of the used dentifrices was also determined by the Hefferren abrasivity test. Results The Ra values increased significantly in all resin composites after 3DW and BW toothbrushing. The acceptable threshold color varied among resin composites, and TNCB and Z100 presented the highest ΔEab and ΔE00 for BW dentifrice. The 3DW dentifrice was significantly more abrasive than BW dentifrice on enamel and dentin. Conclusions simulated toothbrushing with tested whitening dentifrices increased the surface roughness at acceptable levels. The Tetric N Ceram Bulk-fill and Z100 composite showed the highest color alteration in BW. 3D White Perfection dentifrice was more abrasive on dentin and enamel than Black is White. bulkfill bulk fill radioactiverelative radioactive relative (RDA REA n=10 n10 n 10 (n=10 TNCB, , (TNCB) FOB (FOB Z100Control ZControl Control Z Z100(Control matrix Ra, (Ra μm ΔEab, (ΔEab ΔE WID system sample D OralB Oral B DW (3DW (BW test Z10 ΔE0 levels Bulkfill n=1 n1 1 (n=1 (TNCB Z1 n= (n= (n

Aim Hyposalivation and dry mouth affect the quality of life in patients with Head and Neck Cancer, who did the treatment with radiotherapy. Thus this study has the objective to evaluate the dosimetric relationship between 3D radiotherapy and changes in salivary flow, xerostomia and quality of life in patients with head and neck cancer according to the volume of the irradiated parotid gland. Methods 23 patients with cancer in the head and neck area and in need of 3D radiotherapy were followed up during radiotherapy treatment, and the parotid gland (PG) design was also performed in radiotherapy planning. Questionnaires were carried out to determine xerostomia and quality of life, while the salivary flow was determined through calculations regarding the collection and weighing of saliva. Such data were collected in three moments: before the beginning of the radiotherapy treatment (D0), in the middle of the treatment (D1) and at the end of it (D2). The numerical variables are represented by measures of central tendency and measures of dispersion. Results when associating the salivary flow, the xerostomia questionnaire and the OHIP-14, a statistically significant difference was found (p-value <0.001), as well as when comparing some volumes of irradiated PG with the OHIP-14. However, no relationship was found between dosimetric data, xerostomia and hyposalivation. Conclusion patients undergoing 3D radiotherapy for malignant neoplasms in the head and neck region had decreased salivary flow, increased complaints of dry mouth and decreased quality of life. However, it was not possible to establish a statistically significant correlation between these findings and the volumes of irradiated parotids. Cancer D 2 (PG planning saliva moments D0, D0 , (D0) D1 (D1 D2. D2 . (D2) dispersion OHIP14, OHIP14 OHIP 14, 14 OHIP-14 pvalue p value <0.001, 0001 <0.001 0 001 <0.001) OHIP14. 14. However hyposalivation parotids (D0 (D (D2 OHIP1 1 OHIP-1 000 <0.00 00 OHIP- <0.0 <0. <0 <

Aim This study aims to report the perioperative management of a patient with von Willebrand disease (vWd) who underwent orthognathic surgery. The report follows the guidelines of the Case Report Guidelines (CARE) and focuses on the steps taken to prevent bleeding during the surgical procedure. Methods A 39-year-old female patient with skeletal Class III was treated with maxillary advancement and mandibular setback. Despite normal test results for ristocetin cofactor activity, measures were taken to prevent bleeding, including atraumatic surgical techniques, use of antifibrinolytic medication, induced hypotension during anesthesia, and preparation of blood products for transfusion during trans and postoperative periods if needed. In the end, these measures were not required. Results The patient did not experience any bleeding during the surgical procedure or postoperative period, demonstrating the effectiveness of the measures taken to manage their blood dyscrasia. Two years after the surgery, the patient had satisfactory aesthetic and functional results and no evidence of relapse. Conclusion Thus, this case report demonstrates that vWd does not prevent large-scale oral and maxillofacial surgeries such as orthognathic surgery as long as proper precautions are taken pre-, intra- and postoperatively. (vWd CARE (CARE 39yearold yearold 39 year old setback activity techniques medication anesthesia needed end required period dyscrasia relapse Thus largescale large scale pre, pre , pre- intra postoperatively 3

Aim The study aimed to evaluate the association between oral health and academic performance and/or school absenteeism. Methods Electronic searches were performed of the PubMed, Web of Science, SCOPUS and LILACS/BVS databases. We included observational studies that evaluated the association between dental caries, tooth loss, dental pain or oral health status with school absenteeism or academic performance. The studies had to contain a representative sample of the population: schoolchildren, children or adolescents. After the removal of duplicates, the electronic searches produced 3,789 articles. Of these, 25 studies were included in the systematic review and 13 in the meta-analysis. Results Considering all the studies evaluated in the meta-analysis, seven articles satisfied 100% of the Joanna Briggs Institute Critical Appraisal checklist, and six contained between 90% and 75% positive answers. The pooled effects showed that the chances of school absenteeism were 31% higher in subjects with dental caries (OR 1.31; 95%CI 1.12-1.54). Students with fair/poor dental health had a 50% higher chance of suffering poor academic performance (OR 1.50; 95%CI 1.22-1.84) and 34% higher chance of having problems at school (OR 1.34; 95%CI 1.06-1.70). Students with a history of toothache had a 3.7 higher chance of being absent from school (OR 6.65; 95%CI 1.60-8.32) and 71% higher chance of missing class due to toothache (OR 1.71; 95%CI 1.15-2.56). Subjects with a history of toothache had a 2.5 times higher chance of suffering poor academic performance (OR 2.58; 95% CI 2.04-3.27). Conclusion Therefore, students with oral problems were more likely to take time off school and present inferior academic performance. andor PubMed Science LILACSBVS LILACS BVS databases loss population schoolchildren adolescents duplicates 3789 3 789 3,78 these 2 1 metaanalysis. metaanalysis meta analysis. analysis meta-analysis metaanalysis, analysis, 100 checklist 90 75 answers 31 OR 1.31 131 95CI 95 1.121.54. 112154 1.12 1.54 . 12 54 1.12-1.54) fairpoor fair 50 1.50 150 1.221.84 122184 1.22 1.84 22 84 1.22-1.84 34 1.34 134 1.061.70. 106170 1.06 1.70 06 70 1.06-1.70) 37 7 3. 6.65 665 6 65 1.608.32 160832 1.60 8.32 60 8 32 1.60-8.32 71 1.71 171 1.152.56. 115256 1.15 2.56 15 56 1.15-2.56) 5 2. 2.58 258 58 2.043.27. 204327 2.04 3.27 04 27 2.04-3.27) Therefore 378 78 3,7 10 9 1.3 121 1.121.54 11215 112 1.1 154 1.5 1.12-1.54 221 1.221.8 12218 122 1.2 184 1.8 1.22-1.8 061 1.061.70 10617 106 1.0 170 1.7 0 1.06-1.70 6.6 66 608 1.608.3 16083 160 1.6 832 8.3 1.60-8.3 17 152 1.152.56 11525 115 256 1.15-2.56 043 2.043.27 20432 204 2.0 327 3.2 2.04-3.27 3, 1. 1.121.5 1121 11 1.12-1.5 1.221. 1221 18 1.22-1. 1.061.7 1061 1.06-1.7 6. 1.608. 1608 16 83 8. 1.60-8. 1.152.5 1152 1.15-2.5 2.043.2 2043 20 2.04-3.2 1.121. 1.12-1. 1.221 1.22-1 1.061. 1.06-1. 1.608 1.60-8 1.152. 1.15-2. 2.043. 2.04-3. 1.121 1.12-1 1.22- 1.061 1.06-1 1.60- 1.152 1.15-2 2.043 2.04-3 1.12- 1.06- 1.15- 2.04-

Aim This study aimed to investigate the occurrence of enamelin gene (ENAM) single nucleotide polymorphisms (SNP) and ENAM polymorphism association with dental anomalies (DA) in individuals with unilateral or bilateral cleft lip and palate (CLP). Methods Saliva samples were collected from 147 individuals aged between 6 and 15 years-old, both genders, and divided into 4 groups: Group 1 (G1) - CLP and DA; Group 2 (G2) - CLP without DA; Group 3 (G3) - without CLP with DA; Group 4 (G4) - without CLP and DA. The genomic DNA was extracted from saliva samples and the following ENAM SNPs markers were genotyped: rs3796703, rs3796704, rs3796705, rs7671281, rs2609428, and rs35951442. Fisher exact and Pearson’s Chi-square tests statistically analyzed the results (α=5%). Results Individuals without CLP with DA (Group 3 - 19.2%) showed statistically higher prevalence of SNP rs2609428 heterozygotes (p=0.006) than individuals with CLP and DA (Group 1 - 0%). Individuals without CLP (10%) exhibited statistically higher prevalence of mutated heterozygotes/homozygous (p=0.028) than in individuals with CLP (1.3%). Conclusion SNP rs2609428 marker of ENAM gene may be associated with dental anomalies in individuals without cleft lip and palate. (ENAM (SNP (DA CLP. . (CLP) 14 yearsold, yearsold years old, old years-old genders groups G1 G (G1 G2 (G2 G3 (G3 G4 (G4 genotyped rs3796703 rs rs3796704 rs3796705 rs7671281 rs35951442 Pearsons Pearson s Chisquare Chi square α=5%. α5 α α=5% 5 (α=5%) 19.2% 192 19 rs260942 p=0.006 p0006 p 0 006 (p=0.006 0%. 0% 0%) 10% 10 (10% heterozygoteshomozygous homozygous p=0.028 p0028 028 (p=0.028 1.3%. 13 1.3% (1.3%) (CLP (G rs379670 rs767128 rs3595144 α=5 (α=5% 19.2 rs26094 p=0.00 p000 00 (p=0.00 (10 p=0.02 p002 02 (p=0.02 1.3 (1.3% rs37967 rs76712 rs359514 α= (α=5 19. rs2609 p=0.0 p00 (p=0.0 (1 1. (1.3 rs3796 rs7671 rs35951 (α= rs260 p=0. p0 (p=0. ( (1. rs379 rs767 rs3595 (α rs26 p=0 (p=0 rs37 rs76 rs359 rs2 p= (p= rs3 rs7 rs35 (p

Aim Venous blood derivatives (VBDs) have been suggested as substitutes for Fetal Bovine Serum (FBS) to improve the clinical transition of cell-based therapies. The literature is not clear about which is the best VBDs substitute. The present study aimed to evaluate the influence of VBDs on cell viability and describe a new method to seed these cells in a 3D Platelet-Rich Fibrin (PRF). Methods Blood was processed to obtain Platelet-Poor Plasma from PRF (P-PRF), Human Serum (HS), Platelet-Poor Plasma from PRP (P-PRP), activated-PRP (a-PRP), and Platelet lysate (PL). Cells were supplemented with each VBD at 10% and FBS at 10% was the control. Cell viability (fibroblast 3T3/NIH) test was evaluated with MTT assay in two ways: i) cell-seeded and expanded with VBD; ii) cell-seed with FBS and expanded with VBD. To seed the Fibrin construct, cells were suspended in PBS and dropped into the blood sample before performing Choukroun’s protocol for PRF. Constructs were cultured for 7 days in VBD supplements and FBS. Histological and Immunohistochemical analysis with vimentin was performed. Cell viability was analyzed by one-way ANOVA. Results VBD’s production time was very heterogeneous. Cells expanded in HS and a-PRP has grown faster. VBD-supplemented culture media provided cell culture highly sensible to trypsin/EDTA 0.25%. Cells seeded and expanded with VBD presented viability comparable to FBS in HS, a-PRP, and P-PRP (p>0.05) and lower in P-PRF and PL groups (p<0.05). The viability of cell seed with FBS and expanded with VBD was similar between P-PRF, a-PRP, PL, and FBS (p>0.05) and lower in HS and P-PRP (p<0.005). PRF-seeded cells showed a positive expression of vimentin and were able to maintain all cells supplemented with VBD. Conclusion VBD supplements were able to maintain fibroblast cells in 2D and 3D cultures. The new method of the fibrin-cell construct was efficient to insert the cells into the fibrin network. (VBDs (FBS cellbased based therapies substitute D PlateletRich Rich . (PRF) PlateletPoor Poor PPRF, PPRF P , (P-PRF) (HS) PPRP, PPRP (P-PRP) activatedPRP activated aPRP, aPRP (a-PRP) PL. (PL) 10 control 3T3/NIH 3T3NIH TNIH 3T3 NIH T ways i cellseeded ii cellseed Choukrouns Choukroun s performed oneway one way ANOVA heterogeneous faster VBDsupplemented trypsinEDTA trypsin EDTA 025 0 25 0.25% PRP, p>0.05 p005 p 05 (p>0.05 p<0.05. p<0.05 (p<0.05) PRF, p<0.005. p0005 p<0.005 005 (p<0.005) PRFseeded cultures fibrincell network (PRF (P-PRF (HS (P-PRP (a-PRP (PL 1 3T 02 2 0.25 p>0.0 p00 (p>0.0 p<0.0 (p<0.05 p000 p<0.00 00 (p<0.005 0.2 p>0. p0 (p>0. p<0. (p<0.0 (p<0.00 0. p>0 (p>0 p<0 (p<0. p> (p> p< (p<0 (p (p<

Aim This study aimed to compare the influence of sodium hypochlorite (NaOCl) and calcium hypochlorite [Ca(OCl)2] on the apical sealing capacity of AH Plus (Dentsply Maillefer) and Bio-C Sealer (Angelus) endodontic sealers. Methods Sixty permanent human lower incisors were randomly allocated (http://www.randomized.org), according to the irrigant used, into three groups (n=20): 0.9% sodium chloride (NaCl/Control); 2.5% NaOCl; and 2.5% Ca(OCl)2. The root canal was prepared with rotary files under 10 mL of the solution corresponding to the experimental group. Each group was subdivided into two (n=10) according to the sealer used for filling: AH Plus (Dentsply Maillefer) or Bio-C Sealer (Angelus). Then, all samples were immersed in black India ink for one week. After the storage period, the roots were then grooved longitudinally and split, and the ink penetration was measured from the apical part to the coronal part of the root canal into which the ink penetrated using a stereomicroscope. Data were analyzed by one-way ANOVA and Tukey’s post-hoc tests. Results There was no statistical difference in ink penetration between the different endodontic sealers tested for the same irrigating solution (p > 0.05). However, when the Bio-C Sealer (Angelus) was used, the group treated with 2.5% Ca(OCl)2 was associated with lower values of apical leakage, compared to 2.5% NaOCl (p < 0.05). For the AH Plus sealer (Dentsply Maillefer), there was no difference between the irrigants (p > 0.05). Conclusions Associating Ca(OCl)2 irrigant with Bio-C Sealer (Angelus) seems to be a good option to reduce apical leakage. (NaOCl CaOCl2 CaOCl Ca OCl 2 [Ca(OCl)2 Dentsply Maillefer BioC Bio C Angelus (Angelus http//www.randomized.org, httpwwwrandomizedorg http //www.randomized.org , www randomized org (http://www.randomized.org) n=20 n20 n 20 (n=20) 09 0 9 0.9 NaCl/Control NaClControl NaCl Control (NaCl/Control) 25 5 2.5 CaOCl2. 2. 1 n=10 n10 (n=10 filling Angelus. . Then week period split stereomicroscope oneway way Tukeys Tukey s posthoc post hoc tests p 0.05. 005 0.05 05 0.05) However Ca(OCl) leakage Maillefer, [Ca(OCl) http//www.randomized.org wwwrandomizedorg (http://www.randomized.org n=2 n2 (n=20 0. (NaCl/Control n=1 n1 (n=1 00 0.0 Ca(OCl [Ca(OCl n= (n=2 (n= (n

Aim To assess the opinion of the students on the impact of COVID-19 pandemic on theoretical knowledge and clinical practice in dentistry at the Piracicaba Dental School – FOP/UNICAMP. Methods A questionnaire was applied using the Google Forms platform, containing 20 questions related to the impacts of the pandemic on knowledge, mental health, and clinical and laboratory practice of dentistry. The satisfaction of the students with teaching was also evaluated. A total of 120 questionnaires were analyzed using R software, through tables and graphs of absolute and relative frequencies distribution. Results COVID-19 affected the lives of 99% students who participated in the study. Due to distance learning resulting from the COVID-19 pandemic, 50% of the students considered locking or dropping out of college. Operative dentistry was the curricular component most affected by distance and lack of clinical practice. Although most students agreed that the workload of practical disciplines was or would be replaced, 95% felt some kind of deficit in clinical and laboratory practice even with the replacement of the workload. In addition, 93.3% of the students were afraid of not becoming a qualified professional due to the deficiencies on theoretical knowledge and clinical practice caused by the pandemic. Conclusions Students showed dissatisfaction with the deficiency of clinical and laboratory practice resulting from the pandemic in operative dentistry curricular component. They reported fear and insecurity with their future professional lives. The indication of remote classes for dentistry should only be carried out in emergencies because this is an essentially practical course that suffers losses in learning. COVID19 COVID 19 COVID-1 FOPUNICAMP FOP UNICAMP FOP/UNICAMP platform 2 health evaluated 12 software distribution 99 study 50 college replaced 95 addition 933 93 3 93.3 COVID1 1 COVID- 9 5 93.