Abstract Introduction Malignant Hyperthermia (MH) is a pharmacogenetic, hereditary and autosomal dominant syndrome triggered by halogenates/succinylcholine. The In Vitro Contracture Test (IVCT) is the gold standard diagnostic test for MH, and it evaluates abnormal skeletal muscle reactions of susceptible individuals (earlier/greater contracture) when exposed to caffeine/halothane. MH susceptibility episodes and IVCT seem to be related to individual features. Objective To assess variables that correlate with IVCT in Brazilian patients referred for MH investigation due to a history of personal/family MH. Methods We examined IVCTs of 80 patients investigated for MH between 2004‒2019. We recorded clinical data (age, sex, presence of muscle weakness or myopathy with muscle biopsy showing cores, genetic evaluation, IVCT result) and IVCT features (initial and final maximum contraction, caffeine/halothane concentration triggering contracture of 0.2g, contracture at caffeine concentration of 2 and 32 mmoL and at 2% halothane, and contraction after 100 Hz stimulation). Results Mean age of the sample was 35±13.3 years, and most of the subjects were female (n=43 or 54%) and MH susceptible (60%). Of the 20 subjects undergoing genetic investigation, 65% showed variants in RYR1/CACNA1S genes. We found no difference between the positive and negative IVCT groups regarding age, sex, number of probands, presence of muscle weakness or myopathy with muscle biopsy showing cores. Regression analysis revealed that the best predictors of positive IVCT were male sex (+12%), absence of muscle weakness (+20%), and personal MH background (+17%). Conclusions Positive IVCT results have been correlated to male probands, in accordance with early publications. Furthermore, normal muscle strength has been confirmed as a significant predictor of positive IVCT while investigating suspected MH cases.

Resumo Objetivos investigar a compreensão de enfermeiras assistenciais sobre espiritualidade; analisar o cuidado espiritual prestado pelas enfermeiras à mãe de bebê com malformação, à luz da Teoria de Jean Watson. Método abordagem qualitativa, implementando a entrevista semiestruturada com 11 enfermeiras de uma maternidade de referência no município de João Pessoa (PB). Os dados foram analisados a partir da técnica da análise de conteúdo proposta por Bardin. Resultados emergiram duas categorias: compreensão de enfermeiras sobre espiritualidade e cuidado espiritual; cuidado espiritual prestado pelas enfermeiras às mães de bebês com malformação congênita, na perspectiva da Teoria de Jean Watson. As profissionais utilizam, empiricamente, elementos do Processo Clinical Caritas-Veritas. Conclusão e implicações para a prática as enfermeiras compreendem espiritualidade como algo que norteia e dá sentido à vida, podendo ou não estar relacionada a uma prática religiosa. Reconhecem que o cuidado espiritual auxilia as mães no enfrentamento de problemas.

Abstract Objectives to investigate the understanding of nursing assistants on spirituality; to analyze the spiritual care provided by nurses to the mother of a baby with a malformation in the light of Watson’s Theory. Method this was a qualitative approach implementing semi-structured interviews with eleven nurses from a reference maternity hospital in the city of João Pessoa (Paraíba State, northeastern Brazil). Data were analyzed using the content analysis technique proposed by Bardin. Results two categories emerged: the nurses’ understanding of spirituality and spiritual care and the spiritual care provided by the nurses to the mothers of malformed babies from the perspective of Watson’s theory. The professionals empirically use elements of the Caritas-Veritas Clinical Process. Conclusion and implications for the practice the nurses understand spirituality as something that guides and brings meaning to life, whether or not it is linked to religious practice. They recognize that the spiritual care provided to the mothers of malformed babies helps them cope with the problem.

Resumen Objetivos Investigar la comprensión de enfermeros asistenciales sobre espiritualidad; analizar el cuidado espiritual que prestan las enfermeras a la madre de un bebé con malformación, a la luz de la Teoría de Jean Watson. Método abordaje cualitativo implementando una entrevista semiestructurada con once enfermeras de una maternidad de referencia en la ciudad de João Pessoa (Estado de Paraíba, noreste de Brasil). Los datos se analizaron mediante la técnica de análisis de contenido propuesta por Bardin. Resultados asomaron dos categorías: comprensión del enfermero sobre espiritualidad y cuidado espiritual y el cuidado espiritual ofrecido por los enfermeros a las madres de bebés malformados en la perspectiva de la teoría de Jean Watson. Los profesionales utilizan empíricamente elementos del Proceso Clínico Caritas-Veritas. Conclusión e implicaciones para la práctica Los enfermeros comprenden espiritualidad como algo que orienta y ofrece sentido a la vida, pudiendo o no estar ligada a una práctica religiosa. Reconocen que el cuidado espiritual ofrecido a las madres de bebés malformados las auxilia en el enfrentamiento del problema.

ABSTRACT Background: Seasonally Dry Tropical Forests (SDTF) are recognized by their great biological diversity, but little is known about their temporal patterns, which may be crucial in a changing world. Here we aimed to characterize the SDTF dynamic patterns in floristic and structural changes, and also to evaluate shifts in dynamic rates and structural attributes such as richness, number of trees and biomass. Our hypothesis is that SDTF tree communities have their dynamic characterized by temporal fluctuations related to an instability pattern, in according to the already found for others tropical forests. For this we used a data of 42 400 m² plots in three fragments in the Brazilian Arboreal Caatinga, measured in 2005, 2010 and 2015. We evaluated temporal changes in richness, species composition, trees density, biomass and rates of tree mortality and recruitment; and also rates of biomass gain and loss. Results: We found a pattern of floristic composition stability, but with changes in structure and dynamics. There was a tree density decrease driven by constant mortality and by recruitment decrease, and biomass increase due to decrease in biomass loss and to increase in biomass gain. The biomass increase was main related to a small set of dominant species that are the most representative in the community dynamics. Conclusion: SDTF dynamics are related to stability in species composition, but with structural changes towards higher biomass stocks. The results are main related to community dynamics and to SDTF attributes such as climatic seasonality that drives the local ecological processes.

Abstract This work proposes the application of a slot in the ground plane, Defected Ground Structure (DGS), of a microstrip patch antenna. The application of this technique aims to attenuate higher-order excited modes without deteriorating the antenna parameters in the fundamental mode. The methodology for the design of the slot in H-format is presented, based on the physical dimensions of the microstrip patch antenna. The positioning of the DGS concerning the feed line was determined from a detailed parametric analysis. The antenna was designed for the fundamental mode at 2.45 GHz frequency. Two antenna prototypes were built, with and without DGS, and measurements of reflection coefficient, gain, and radiation pattern were performed. The measured results show that the application of DGS considerably attenuated higher-order excited modes. A good agreement between simulated and measured results is achieved.

RESUMO A Noticia Primeira Practica Que da ao Reverendo Padre Diogo Soarez o Cappitam Ioaõ Antonio Cabral Camello sobre a Viage, que fez as Minaz do Cuyaba no anno de 1727 consiste numa narrativa acerca da viagem fluvial empreendida por João Antonio Cabral Camello de Sorocaba (SP) a Cuiabá (MT) no ano de 1727, possivelmente escrita em 1734. Os dois testemunhos manuscritos conhecidos desse texto encontram-se no códice CXVI 1-15 da Coleção Diogo Soares, da Biblioteca Pública de Évora (Portugal). Ao longo dos séculos subsequentes, a Noticia foi publicada em diferentes meios, como na Revista do Instituto Histórico e Geográfico Brasileiro, por Francisco Adolfo de Varnhagen, em 1842, e no livro Relatos monçoeiros, de 1953, organizado por Afonso d’Escragnolle Taunay. Este artigo tem por objetivo traçar a trajetória histórica da Noticia Primeira Practica por meio do estudo de sua produção, transmissão e apropriação ao longo dos séculos XVIII, XIX e XX. A natureza do objeto, bem como as questões investigativas propostas, nos impelem a lançar mão das contribuições teóricas de diversos campos do conhecimento, tais como a história da cultura escrita, a paleografia, a filologia e a bibliografia material. Embora distintas, essas áreas possuem um objeto de pesquisa em comum: o texto escrito, estudado enquanto representação histórica, social, linguística e material. O reconhecimento da dimensão material do texto nos possibilita analisá-lo sob a perspectiva dos estudos de cultura material. Sendo antes de mais nada um artefato, a Noticia Primeira Practica será considerada enquanto vetor e produto de relações sociais.

ABSTRACT The “Noticia Primeira Practica Que da ao Reverendo Padre Diogo Soarez o Cappitam Ioaõ Antonio Cabral Camello sobre a Viage, que fez as Minaz do Cuyaba no anno de 1727” consists of a narrative about the river trip undertaken by João Antonio Cabral Camello from Sorocaba (São Paulo) to Cuiabá (Mato Grosso) in 1727, possibly written in 1734. The two existing handwritten testimonies concerning this text can be found in codex CXVI 1-15 of the Diogo Soares Collection of the Évora Public Library, Portugal. Over the following centuries it was published in different media, such as the Revista do Instituto Histórico e Geográfico Brasileiro by Francisco Adolfo de Varnhagen, in 1842, and the book Relatos monçoeiros in 1953, organized by Afonso d’Escragnolle Taunay. This article traces the historical trajectory of Noticia Primeira Practica by studying its production, transmission and appropriation throughout the 18th, 19th and 20th centuries. Due to the nature of the object, as well as the proposed investigative questions, its study requires an interdisciplinary theoretical framework including History of Written Culture, Palaeography, Philology and Material Bibliography. Although distinct, these fields have a common research object: written text studied as a historical, social, linguistic and material representation. Acknowledging the material dimension of the text allowed us to analyze it from the perspective of Material Culture studies. Primarily an artifact, the Noticia Primeira Practica is here to considered a vector and product of social relations.

PURPOSE: To assess the mutagenic potential of the oxygen inhalation therapy (HBO), by means of the micronucleus test, performed in peripheral blood of rats that underwent subtotal splenectomy with lower pole preservation (ESTPI), after HBO sessions or simulations.METHODS: Eighteen male Wistar rats, were distributed into three groups of six animals: group 1 - submitted to ESTPI and HBO sessions; group 2 - submitted to ESTPI and HBO simulations; group 3 - underwent cyclophosphamide administration. In groups 1 and 2, blood samples from the animals' tails were collected before surgery (T0) and immediately after the 13th HBO session or simulation (T1). In group 3, tail blood samples were collected from animals before (T0) and 24 hours after (T1) cyclophosphamide (CP) delivery. The number of micronucleated normochromatic erythrocytes (MNNCE) was determined by blind counting 2000 normochromatic erythrocytes (NCE) per animal.RESULTS:Micronuclei average after CP delivery in group 3 was higher than before its use, thus confirming the mutagenic activity of this drug (p=0.01). In groups 1 and 2, no significant difference in the average of Micronuclei was observed when comparing it to blood samples before and after the 13th HBO session or simulation.CONCLUSION: The treatment protocol used in this study did not induce Micronucleus formation in animals submitted to ESTPI and HBO treatment or simulation.

A degradação dos recursos hídricos está intensamente relacionada à atividade agrícola. O objetivo deste trabalho foi avaliar a influência do uso do solo e de alguns componentes ambientais na qualidade da água superficial na microbacia do rio Campestre, município de Colombo, Paraná. Foram analisados os atributos físicos e químicos (nitrogênio Kjeldahl, amônio, nitrato, fósforo total, turbidez, pH, temperatura, condutividade elétrica, sólidos totais, demanda biológica de oxigênio, demanda química de oxigênio e oxigênio dissolvido). A coleta das amostras de água foi realizada mensalmente por um ano em oito pontos, distribuídos em três sub-bacias. De modo geral, a qualidade da água foi inferior na sub-bacia com maior porcentagem de agricultura, sendo influenciada também pelas porcentagens de floresta, área de preservação permanente com agricultura e área de contribuição da sub-bacia. Na estação de verão, provavelmente em razão da maior precipitação pluvial e do uso do solo, ocorreram as maiores concentrações de N Kjeldahl e N particulado, bem como de condutividade elétrica, pH e turbidez. Todos os atributos analisados, exceto o P total, estavam de acordo com a Resolução Conama nº 357/2005, para água doce de classe 1. No entanto, cabe ressaltar que o monitoramento realizado referiu-se ao escoamento de base e não representou uma condição de descarga, já que a maioria das coletas não foi realizada durante ou próxima do evento de precipitação.

Water degradation is strongly related to agricultural activity. The aim of this study was to evaluate the influence of land use and some environmental components on surface water quality in the Campestre catchment, located in Colombo, state of Parana, Brazil. Physical and chemical attributes were analyzed (total nitrogen, ammonium, nitrate, total phosphorus, electrical conductivity, pH, temperature, turbidity, total solids, biological oxygen demand, chemical oxygen demand and dissolved oxygen). Monthly samples of the river water were taken over one year at eight monitoring sites, distributed over three sub-basins. Overall, water quality was worse in the sub-basin with a higher percentage of agriculture, and was also affected by a lower percentage of native forest and permanent preservation area, and a larger drainage area. Water quality was also negatively affected by the presence of agriculture in the riparian zone. In the summer season, probably due to higher rainfall and intensive soil use, a higher concentration of total nitrogen and particulate nitrogen was observed, as well as higher electrical conductivity, pH and turbidity. All attributes, except for total phosphorus, were in compliance with Brazilian Conama Resolution Nº 357/2005 for freshwater class 1. However, it should be noted that these results referred to the base flow and did not represent a discharge condition since most of the water samples were not collected at or near the rainfall event.