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Fernandes, Fabio Simões, Marcus V. Correia, Edileide de Barros Marcondes-Braga, Fabiana G. Coelho-Filho, Otavio Rizzi Mesquita, Cláudio Tinoco Mathias-Junior, Wilson Rochitte, Carlos Eduardo Ramires, Felix José Alvarez Alves, Silvia Marinho Martins Montera, Marcelo Westerlund Lopes, Renato Delascio Oliveira-Junior, Mucio Tavares Scolari, Fernando L. Avila, Walkiria Samuel Canesin, Manoel Fernandes Bacal, Fernando Bocchi, Edimar Alcides Moura, Lídia Ana Zytynski Saad, Eduardo Benchimol Scanavacca, Mauricio I. Valdigem, Bruno Pereira Cano , Manuel Nicolas Abizaid , Alexandre Ribeiro, Henrique Barbosa Lemos-Neto, Pedro Alves Ribeiro, Gustavo Calado de Aguiar Jatene, Fabio Biscegli Dias, Ricardo Ribeiro Beck-da-Silva, Luis Rohde, Luis Eduardo P. Bittencourt, Marcelo Imbroinise Pereira, Alexandre Krieger, José Eduardo Villacorta, Humberto Martins, Wolney de Andrade Figueiredo-Neto, José Albuquerque de Cardoso , Juliano Novaes Pastore, Carlos Alberto Jatene, Ieda Biscegli Tanaka, Ana Cristina Sayuri Hotta, Viviane Tiemi Romano, Minna Moreira Dias Albuquerque, Denilson Campos de Mourilhe-Rocha, Ricardo Hajjar, Ludhmila Abrahão Brito, Fabio Sandoli de Caramelli , Bruno Calderaro, Daniela Farsky, Pedro Silvio Colafranceschi , Alexandre Siciliano Pinto, Ibraim Masciarelli Vieira , Marcelo Luiz Campos Danzmann, Luiz Claudio Barberato , Silvio Henrique Mady, Charles Martinelli-Filho, Martino Torbey , Ana Flavia Malheiros Schwartzmann, Pedro Vellosa Macedo, Ariane Vieira Scarlatelli Ferreira , Silvia Moreira Ayub Schmidt, Andre Melo , Marcelo Dantas Tavares de Lima-Filho, Moysés Oliveira Sposito, Andrei C. Brito, Flavio de Souza Biolo, Andreia Madrini-Junior, Vagner Rizk, Stéphanie Itala Mesquita, Evandro Tinoco

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Data de publicação: 2024-04-08

Resumo: Pt Es En

A cardiomiopatia hipertrófica (CMH) é uma forma de doença do músculo cardíaco de causa genética, caracterizada pela hipertrofia das paredes ventriculares. O diagnóstico requer detecção por métodos de imagem (Ecocardiograma ou Ressonância Magnética Cardíaca) de qualquer segmento da parede do ventrículo esquerdo com espessura > 15 mm, sem outra causa provável. A análise genética permite identificar mutações de genes codificantes de diferentes estruturas do sarcômero responsáveis pelo desenvolvimento da CMH em cerca de 60% dos casos, permitindo o rastreio de familiares e aconselhamento genético, como parte importante do manejo dos pacientes e familiares. Vários conceitos sobre a CMH foram recentemente revistos, incluindo sua prevalência de 1 em 250 indivíduos, não sendo, portanto, uma doença rara, mas subdiagnosticada. A vasta maioria dos pacientes é assintomática. Naqueles sintomáticos, a obstrução do trato de saída do ventrículo esquerdo (OTSVE) é o principal distúrbio responsável pelos sintomas, devendo-se investigar a sua presença em todos os casos. Naqueles em que o ecocardiograma em repouso ou com Manobra de Valsalva não detecta gradiente intraventricular significativo (> 30 mmHg), devem ser submetidos à ecocardiografia com esforço físico para detecção da OTSVE.   Pacientes com sintomas limitantes e grave OTSVE, refratários ao uso de betabloqueadores e verapamil, devem receber terapias de redução septal ou uso de novas drogas inibidoras da miosina cardíaca. Por fim, os pacientes adequadamente identificados com risco aumentado de morta súbita podem receber medida profilática com implante de cardiodesfibrilador implantável (CDI).

La miocardiopatía hipertrófica (MCH) es una forma de enfermedad cardíaca de origen genético, caracterizada por el engrosamiento de las paredes ventriculares. El diagnóstico requiere la detección mediante métodos de imagen (Ecocardiograma o Resonancia Magnética Cardíaca) que muestren algún segmento de la pared ventricular izquierda con un grosor > 15 mm, sin otra causa probable. El análisis genético permite identificar mutaciones en genes que codifican diferentes estructuras del sarcómero responsables del desarrollo de la MCH en aproximadamente el 60% de los casos, lo que permite el tamizaje de familiares y el asesoramiento genético, como parte importante del manejo de pacientes y familiares. Varios conceptos sobre la MCH han sido revisados recientemente, incluida su prevalencia de 1 entre 250 individuos, por lo tanto, no es una enfermedad rara, sino subdiagnosticada. La gran mayoría de los pacientes son asintomáticos. En los casos sintomáticos, la obstrucción del tracto de salida ventricular izquierdo (TSVI) es el trastorno principal responsable de los síntomas, y su presencia debe investigarse en todos los casos. En aquellos en los que el ecocardiograma en reposo o la maniobra de Valsalva no detecta un gradiente intraventricular significativo (> 30 mmHg), deben someterse a ecocardiografía de esfuerzo para detectar la obstrucción del TSVI. Los pacientes con síntomas limitantes y obstrucción grave del TSVI, refractarios al uso de betabloqueantes y verapamilo, deben recibir terapias de reducción septal o usar nuevos medicamentos inhibidores de la miosina cardíaca. Finalmente, los pacientes adecuadamente identificados con un riesgo aumentado de muerte súbita pueden recibir medidas profilácticas con el implante de un cardioversor-desfibrilador implantable (CDI).

Hypertrophic cardiomyopathy (HCM) is a form of genetically caused heart muscle disease, characterized by the thickening of the ventricular walls. Diagnosis requires detection through imaging methods (Echocardiogram or Cardiac Magnetic Resonance) showing any segment of the left ventricular wall with a thickness > 15 mm, without any other probable cause. Genetic analysis allows the identification of mutations in genes encoding different structures of the sarcomere responsible for the development of HCM in about 60% of cases, enabling screening of family members and genetic counseling, as an important part of patient and family management. Several concepts about HCM have recently been reviewed, including its prevalence of 1 in 250 individuals, hence not a rare but rather underdiagnosed disease. The vast majority of patients are asymptomatic. In symptomatic cases, obstruction of the left ventricular outflow tract (LVOT) is the primary disorder responsible for symptoms, and its presence should be investigated in all cases. In those where resting echocardiogram or Valsalva maneuver does not detect significant intraventricular gradient (> 30 mmHg), they should undergo stress echocardiography to detect LVOT obstruction. Patients with limiting symptoms and severe LVOT obstruction, refractory to beta-blockers and verapamil, should receive septal reduction therapies or use new drugs inhibiting cardiac myosin. Finally, appropriately identified patients at increased risk of sudden death may receive prophylactic measure with implantable cardioverter-defibrillator (ICD) implantation.

https://doi.org/10.1590/SciELOPreprints.8394

AVANÇOS NA PRODUÇÃO E FORMULAÇÃO DE INOCULANTES MICROBIANOS VISANDO UMA AGRICULTURA MAIS SUSTENTÁVEL
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Florencio, Camila

; Bortoletto-Santos, Ricardo

; Favaro, Camila P.

; Brondi, Mariana G.

; Velloso, Camila C. V.

; Klaic, Rodrigo

; Ribeiro, Caue

; Farinas, Cristiane S.

; Mattoso, Luiz H. C.

Química Nova

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Set 2022, Volume 45 Nº 9 Páginas 1133 - 1145

Resumo: Pt | Texto: Pt | PDF: Pt

The wide application of beneficial microorganisms in agriculture as inoculants to combat pests and diseases and/or to improve soil fertility and the nutrients availability for plants has been considered as an effective and more sustainable alternative than chemical fertilizers and agricultural defensives. However, it is necessary to examine all processing steps of these bio-based products under a more integrated view including the type of microorganisms and the whole production process in order to reduce dependence on synthetic chemical inputs. Here, recent developments on the production and formulation technologies of microbial inoculants and the main types of inoculants currently applied in agriculture are addressed. The different types of microbial formulations are compared with emphasis on the encapsulation technology. Moreover, the application of biofertilizers in seed coating and a new approach to apply biocomposites as fertilizers are discussed, presenting the main challenges and future perspectives to promote more sustainable agriculture practices.

https://doi.org/10.21577/0100-4042.20170909

Fatty Acid Incorporation in the Muscle, Oxidative Markers, Lipid Peroxidation and PPAR-α and SREBP-2 Expression of Zebrafish Fed Linseed Oil and Clove Leaf Essential Oil
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SILVA, THIBÉRIO C. DA

; UTSUNOMIYA, KARINA S.

; CASTRO, PEDRO LUIZ

; ROCHA, JOANA D’ARC M.

; VISENTAINER, JESUI V.

; GASPARINO, ELIANE

; RIBEIRO, RICARDO P.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 2 elocation e20210236

Resumo: En | Texto: En | PDF: En

Abstract The objective of this study is to assess, in zebrafish, the effects of combining linseed oil (LO) and clove leaf essential oil (CLEO) on the incorporation of fatty acids in the muscle, oxidative markers, lipid peroxidation and expression of the PPAR-α (Peroxisome Proliferator-Activated Receptor-α) and the SREBP-2 (Sterol Regulatory Element Binding Protein-2) genes. Six diets were prepared, containing combinations of LO (3, 6 and 9%) and CLEO (0.5 and 1%): 3% LO + 0.5% CLEO; 3% LO + 1% CLEO; 6% LO + 0.5% CLEO; 6% LO + 1% CLEO; 9% LO + 0.5% CLEO; 9% LO + 1% CLEO. Results showed increase in the incorporation of n-3 fatty acids in the muscle concomitantly with the addition of LO and CLEO. The activities of superoxide dismutase and catalase were reduced and the glutathione content had increased. Lipid peroxidation was lower in the treatment with 1% CLEO, regardless of LO content. The expression of the PPAR-α and the SREBP-2 genes was higher in animals fed 9% LO + 0.5% CLEO. Therefore, for a greater incorporation and protection against the oxidative damages of n-3 fatty acids, a combined use of 9% LO with 0.5% CLEO is recommended for zebrafish.

https://doi.org/10.1590/0001-3765202220210236

The frequency of blindness and visual impairment in the central-west region of Brazil
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Ribeiro, Guilherme B.

; Dobri, Giuliano P.

; Abe, Ricardo Y.

Arquivos Brasileiros de Oftalmologia

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Ago 2021, Volume 84 Nº 4 Páginas 402 - 403

Resumo: En | Texto: En | PDF: En

https://doi.org/10.5935/0004-2749.202100104 79 downloads

Thermoregulation mode, substrate temperature and camouflage efficiency affecting defensive behavior of lizards in Amazonia
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SOUZA, Erika

; COELHO, Ana

; SANTOS-JR, Alfredo P.

; KAWASHITA-RIBEIRO, Ricardo Alexandre

; FRAGA, Rafael de

Acta Amazonica

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Dez 2020, Volume 50 Nº 4 Páginas 339 - 345

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Em ectotérmicos, respostas defensivas a predadores geralmente dependem de relações de custo-benefício entre risco de morte e a energia necessária para fugir. Neste estudo, investigamos lagartos na Amazônia para testar a hipótese de que a distância mínima de aproximação de predadores (DAP) é influenciada pela temperatura e camuflagem. Nós testamos a hipótese de que estimativas de DAP para espécies com diferentes modos de termorregulação respondem diferentemente à temperatura e camuflagem. Nós amostramos 35 lagartos de uma espécie heliotérmica e uma não heliotérmica, para as quais simulamos um predador terrestre visualmente orientado. Usando um modelo linear de efeitos fixos, a temperatura afetou positivamente as estimativas de DAP, mas a camuflagem não contribuiu para o modelo. Usando um modelo linear de efeitos mistos, a camuflagem afetou negativamente as estimativas de DAP, independentemente de temperatura. Nossos resultados sugerem que alta exposição a predadores em hábitats abertos pode ser compensada por fuga rápida otimizada por altas temperaturas, e o baixo desempenho de fuga, usualmente causado por temperaturas relativamente mais baixas em habitats sombreados, pode ser compensado pela camuflagem. No entanto, a identificação do melhor preditor de DAP dependeu muito de considerar o modo de termorregulação nos testes de hipóteses, apesar de que os resultados obtidos por ambos os modelos de efeitos fixos e mistos podem ser relevantes para conservação.

ABSTRACT In ectotherms, defensive responses to predators usually depend on cost-benefit relationships between death risk and the energy required to flee. In this study we investigate Amazonian lizards to test the hypothesis that the minimum predator approach distance (PAD) is influenced by temperature and camouflage. We test the hypothesis that PAD estimated for species with different thermoregulation modes respond differently to temperature and camouflage. We sampled 35 lizards of a heliotherm and a non-heliotherm species, for which we simulated a terrestrial visually oriented predator. Using a fixed-effects linear model, temperature positively affected PAD estimates, but the camouflage did not contribute to the model. Using a mixed linear model assuming thermoregulation mode as a random factor, camouflage negatively affected PAD estimates, independently of temperature. Our findings suggest that high exposure to predators in open habitats may be compensated by rapid fleeing optimized by high temperatures, and low fleeing performance, usually caused by relatively low temperatures in shaded habitats, may be compensated by camouflage. However, identifying the best PAD predictor greatly depended on accounting for thermoregulation mode in hypothesis testing, although the results obtained by both fixed and mixed-effects models may be relevant for conservation.

https://doi.org/10.1590/1809-4392201904251 976 downloads

Omega-3 and Omega-6 Determination in Nile Tilapia’s Fillet Based on MicroNIR Spectroscopy and Multivariate Calibration
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Santos, Dayane A. dos

; Coqueiro, Aline

; Gonçalves, Thays R.

; Carvalho, Jonathan C.

; Bezerra Jr., Jailton S.

; Matsushita, Makoto

; Oliveira, Carlos A. L. de

; Março, Paulo H.

; Valderrama, Patrícia

; Ribeiro, Ricardo P.

Journal of the Brazilian Chemical Society

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Set 2020, Volume 31 Nº 9 Páginas 1883 - 1890

Resumo: En | Texto: En | PDF: En

Tilapia (Oreochromis niloticus) is the most cultivated fish species in Brazil. Studies have shown that fish consumption is related to the prevention of cardiovascular diseases due to its high contents of polyunsaturated fatty acids, as omega-3 (n-3) and omega-6 (n-6). In this sense, a new method to determine omega-3 and omega-6 by near-infrared (NIR) spectroscopy and multivariate calibration based on partial least squares (PLS) was proposed. Spectra of fillets of Nile tilapias packed under vacuum were obtained and compared to the reference method (gas chromatography) used for quantification of fatty acids. The outliers were evaluated in the models that were validated by calculation of the parameters for model performance estimation, showing promising results. The appropriate values achieved for accuracy, limits of detection and quantification, indicate that the NIR/PLS models can be an alternative to chromatography in the determination of omega-3 and omega-6 in fillets of Nile tilapia. Furthermore, the spectra acquisition on the vacuum-packed fillet contributes to the NIR/PLS method in terms of cost and quickness.

https://doi.org/10.21577/0103-5053.20200082 510 downloads

Weight and body yield of selectively bred tambaqui (Colossoma macropomum) farmed in different environments
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MARCOS, REBECA

; CORRÊA FILHO, RUY A.C.

; ABREU, JANESSA S. DE

; SERAPHIM, GUILHERME N.

; SILVA, ANA C.C.

; FORNARI, DARCI C.

; RIBEIRO, RICARDO P.

; FERREIRA, YASMIN A.

; GAMA, KAMYLA F.S.

; POVH, JAYME A.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 elocation e20180675

Resumo: En | Texto: En | PDF: En

Abstract The objective of this study was to evaluate the weight and body yield of two families of selectively bred tambaqui farmed in different environments. Two families (FA and FB) were reared in the municipalities (environments) of Santo Antônio de Leverger (MT) and Campo Grande (MS) for 431 days. Pre-bleeding weight, body yield, and the interaction effect between families and environments on these traits were investigated. No interaction effect between the evaluated families and environments was detected on the evaluated traits. Pre-bleeding weight did not differ significantly between the families in MT (FA: 2,421.7g; FB: 2,478.0g) or in MS (FA: 1,138.7g; FB: 1,389.8g), but the fish from MT had a higher (P<0.05) pre-bleeding weight than those farmed in MS. The visceral fat yield (considering the two environments) was higher (P<0.05) in FB family (3.8%) than in FA family (3.3%).Fish from MS showed higher (P<0.05) offal yield (10.6%) and visceral fat yield (3.9%) but a lower clean-trunk yield (70.6%) than the tambaqui farmed in MT (offal: 7.7%; visceral fat: 3.1%; and clean trunk: 72.6%). In conclusion, the MT environment provides higher pre-bleeding weight and clean-trunk yield and lower offal and visceral-fat yields than the MS environment.

https://doi.org/10.1590/0001-3765202020180675 575 downloads

Growth curve of selectively bred and non-selectively bred tambaqui (Colossoma macropomum)
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MARCOS, REBECA

; RIBEIRO, RICARDO P.

; ABREU, JANESSA S. DE

; FORNARI, DARCI C.

; OLIVEIRA, CARLOS A.L. DE

; STREIT JR, DANILO P.

; BARROS, CAIO M.C.A. DE

; LOPERA-BARRERO, NELSON M.

; CORRÊA FILHO, RUY A.C.

; POVH, JAYME A.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 Nº 3 elocation e20190099

Resumo: En | Texto: En | PDF: En

Abstract The aim of this study was to evaluate the growth curve of selectively bred and non-selectively bred tambaqui (Colossoma macropomum). The experiment involved 388 fish (weight: 65.38 ± 20.00 g; age: 217 days), consisting of 252 fish from seven selectively bred families (18 fish per family) and 18 non-selectively bred fish (control group). Groups were placed in two 800-m² tanks. Biometric measurements were taken on nine occasions at 30-day intervals, for a period of 254 days. Weight and morphometric traits were evaluated. To describe the tambaqui growth behavior, we adopted the Gompertz nonlinear regression model. Greater growth (p < 0.05) was observed in selectively bred families compared with control group. Four families stood out with higher (p < 0.05) asymptotic values for weight (F1: 2448.7 g; F7: 2284.7 g; F5 2180.1 g; F4: 2080.5 g; and control: 1808.4 g) and other morphometric traits. None of the selectively bred families (except F5) had a higher growth rate and age at inflection point than the fish from control group. In conclusion, selectively bred and non-selectively bred fish present distinct growth curves, but some families have greatly superior growth.

https://doi.org/10.1590/0001-3765202020190099 527 downloads

Nutritive value of sugarcane silages with different bacterial additives and fermentation periods
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Silva, Ivone Rodrigues da

; Shigaki, Francirose

; Rodrigues, Rosane Cláudia

; Jesus, Ana Paula Ribeiro

; Costa, Clésio dos Santos

; Araújo, Ricardo Alves de

; Sousa Santos, Francisco Naysson

; Mendes, Sanayra da Silva

Revista Brasileira de Saúde e Produção Animal

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2020, Volume 21 elocation e210212020

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se avaliar o valor nutritivo de silagens de cana-de-açúcar com ou sem inoculante, em diferentes períodos de fermentação. O delineamento experimental foi o inteiramente casualizado em arranjo fatorial 3 x 3 (sem inoculante, inoculante 1 e inoculante 2 x três períodos de fermentação, 10; 60 e 90 dias). Analisou-se pH, matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), hemicelulose (HEM) e lignina; e degradabilidade in situ da MS, quanto aos parâmetros a, b e c, degradação potencial (A) e degradabilidade efetiva (DE). Houve diferença para o pH, o período de 90 dias apresentou menor média para ambos inoculantes. Houve interação inoculante x período de fermentação (P<0,05) para o teor de MS, com redução na silagem sem aditivo aos 90 dias. Os teores de PB, HEM, FDA e lignina não foram influenciados pelos tratamentos. A bactéria P. acidipropionici proporcionou menor teor de FDN aos 10 dias e apresentaram maior fração a, degradação potencial e DE. Aos 60 dias não houve variação na fração solúvel, a silagem controle apresentou maior fração b, maior degradação potencial e DE. Aos 90 dias de fermentação, as silagens com L. buchneri apresentaram maior fração a, taxa de degradação e DE e obteve-se maior valor de b na silagem sem inoculante. Os inoculantes são eficientes em manter os teores de MS das silagens durante períodos prolongados de fermentação e manter o valor nutricional do material ensilado.

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.

https://doi.org/10.1590/s1519-9940210212020 878 downloads

10.

Homeopathy improves production and hatching probability of zebrafish eggs
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Lewandowski, Vanessa

; Sary, Cesar

; Campos, Eric C.

; Oliveira, Carlos A.L. de

; Ribeiro, Ricardo P.

; Vargas, Lauro D.

Latin american journal of aquatic research

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Set 2019, Volume 47 Nº 4 Páginas 595 - 601

Resumo: En | Texto: En | PDF: En

ABSTRACT The homeopathy has been practiced for many years, mainly using rodent as a research model. Zebrafish have been used as an animal model in different scientific areas and may represent an important model in studies with this therapy. This paper aimed to evaluate the action of Homeopatila 100® on the growth, the action on the liver and reproduction of zebrafish. The study was carried out with 192 six months old animals. The experimental period was three months, being evaluated the growth, the hepatosomatic and viscerosomatic indexes, the number of hepatocytes per area and the percentage of glycogen in these cells, performing the hepatic histology of males and females. It was evaluated the reproduction of the animals, checking the production and probability of hatching of the eggs. It was found that homeopathy increases the number of hepatocytes and glycogen in females, as well as improved the reproductive indices evaluated.

https://doi.org/10.3856/vol47-issue4-fulltext-2 615 downloads

11.

Speed and Grade Increment During Cardiopulmonary Treadmill Testing: Impact on Exercise Prescription
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Belli, Karlyse C.

; Silva, Paula F. Da

; Franzoni, Leandro T.

; Myers, Jonathan

; Stein, Ricardo

; Ribeiro, Jorge P.

International Journal of Cardiovascular Sciences

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Ago 2019, Volume 32 Nº 4 Páginas 374 - 383

Resumo: En | Texto: En | PDF: En

Abstract Background: Maximal oxygen uptake (VO2max) and both first (VT1) and second (VT2) thresholds have been used as reference points for exercise prescription in different populations. Objective: We aimed to test the hypothesis that exercise prescription, based on VTs determined by treadmill cardiopulmonary exercise testing (CPET), is influenced by the rate of increase in treadmill workload. Methods: Nine healthy individuals underwent two CPETs, followed by two sessions of submaximal exercise, both in randomized order. For the “speed” protocol, there was an increment of 0.1 to 0.3 km.h-1 every 15s. The “grade” incremental protocol increased 1% every 30s and 0.1 km.h-1 every 45s. This was followed by submaximal exercise sessions lasting 40min at an intensity corresponding to heart rate (HR) between the VT1 and VT2. Results: The “speed” protocol resulted in higher VT1 (p = 0.01) and VT2 (p = 0.02) when compared to the “grade” incremental protocol, but there was no effect on VO2max. The target HR for the submaximal exercise sessions was higher in the “speed” protocol compared to the “grade” incremental protocol (p < 0.01) and remained stable during the two steady-state exercise sessions. Blood lactate remained stable during the submaximal exercise sessions, with higher values observed during the “speed” protocol than those “grade” incremental protocol (p < 0.01). Conclusions: Compared to a grade-based protocol, a speed-based protocol resulted in higher VT1 and VT2, which significantly affected cardiorespiratory and metabolic responses to prescribed exercise intensity in healthy young adults.

https://doi.org/10.5935/2359-4802.20190058 923 downloads

12.

Chloroquine and mefloquine resistance profiles are not related to the circumsporozoite protein (CSP) VK210 subtypes in field isolates of Plasmodium vivax from Manaus, Brazilian Amazon
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Pratt-Riccio, Lilian Rose

; Baptista, Bárbara de Oliveira

; Torres, Vanessa Rodrigues

; Bianco-Junior, Cesare

; Perce-Da-Silva, Daiana de Souza

; Riccio, Evelyn Kety Pratt

; Lima-Junior, Josué da Costa

; Totino, Paulo Renato Rivas

; Cassiano, Gustavo Capatti

; Storti-Melo, Luciane Moreno

; Machado, Ricardo Luiz Dantas

; de Oliveira-Ferreira, Joseli

; Banic, Dalma Maria

; Carvalho, Leonardo José de Moura

; Daniel-Ribeiro, Cláudio Tadeu

Memórias do Instituto Oswaldo Cruz

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2019, Volume 114 elocation e190054

Resumo: En | Texto: En | PDF: En

BACKGROUND The central repetitive region (CRR) of the Plasmodium vivax circumsporozoite surface protein (CSP) is composed of a repetitive sequence that is characterised by three variants: VK210, VK247 and P. vivax-like. The most important challenge in the treatment of P. vivax infection is the possibility of differential response based on the parasite genotype. OBJECTIVES To characterise the CSP variants in P. vivax isolates from individuals residing in a malaria-endemic region in Brazil and to profile these variants based on sensitivity to chloroquine and mefloquine. METHODS The CSP variants were determined by sequencing and the sensitivity of the P. vivax isolates to chloroquine and mefloquine was determined by Deli-test. FINDINGS Although five different allele sizes were amplified, the sequencing results showed that all of the isolates belonged to the VK210 variant. However, we observed substantial genetic diversity in the CRR, resulting in the identification of 10 different VK210 subtypes. The frequency of isolates that were resistant to chloroquine and mefloquine was 11.8 and 23.8%, respectively. However, we did not observe any difference in the frequency of the resistant isolates belonging to the VK210 subtypes. MAIN CONCLUSION The VK210 variant is the most frequently observed in the studied region and there is significant genetic variability in the CRR of the P. vivax CSP. Moreover, the antimalarial drug sensitivity profiles of the isolates does not seem to be related to the VK210 subtypes.

https://doi.org/10.1590/0074-02760190054 912 downloads

13.

New technique of intragastric sleeve: viability and survival in a pig model
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RIBEIRO, MARIANA S.

; ZORRON, RICARDO P.A.S.

; SILVA, SAULO JOSÉ Q.

; CADENA, SILVIA M.R.

; ANTUNES, FERNANDA

; SANTOS JUNIOR, MARCELO B. DOS

; MELLO, LUCIANA M.

; IGREJA JUNIOR, HAROLDO JOSÉ S.

; BATISTA, VILSON L.

; SCHEFFER, JUSSARA P.

; OLIVEIRA, ANDRÉ L.A.

Anais da Academia Brasileira de Ciências

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Set 2018, Volume 90 Nº 3 Páginas 3075 - 3080

Resumo: En | Texto: En | PDF: En

Abstract Developing a less invasive, practical and cost-effective operative technique for obesity treatment represents a pressing need for our society. In this way, intragastric single port sleeve by endoplication was tested in six pigs during 18 weeks. Celiotomy was performed with animal placed in dorsal decubitus position. Single port gastrostomy was performed and double tobacco pouch sutures were made in fundic region, making a gastric sleeve. At the end, stomach layers and skin were closed in a conventional manner. Means and the standard deviations of surgical time were calculated. The procedure was simple and all animals survived; there were no significant blood loss and no intra and postoperative complications. The procedure was fast (67.4 minutes). The technique has the advantage of not requiring the use of mechanical sutures, making it less costly. The innovation of this procedure was the use of a single port gastrostomy device to perform an intraluminal sleeve. What made this technique less invasive were the use of a single port, nonmanipulation of the stomach intra-abdominally, ease of execution and no need of pneumoperitoneum. The new technique is acceptable and has reproducible viability, had a short procedure time without intra and postoperative complications.

https://doi.org/10.1590/0001-3765201820180199 866 downloads

14.

Fatty Acid Composition and Carotenoids in Raw and Grilled Tilapia GIFT Fillets Supplemented with Lycopene, β-Carotene and Lutein
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Reis, Elaine C.

; Galuch, Marília B.

; Silveira, Roberta da

; Santos, Hevelyse M. C. dos

; Sary, Cesar

; Magon, Thiago F. S.

; Figueiredo, Ingrid L.

; Ribeiro, Ricardo P.

; Visentainer, Jesuí V.

; Santos, Oscar O.

Journal of the Brazilian Chemical Society

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Ago 2018, Volume 29 Nº 8 Páginas 1742 - 1750

Resumo: En | Texto: En | PDF: En

This study evaluated the incorporation of β-carotene, lutein and lycopene into tilapia (genetically improved farmed tilapia, GIFT) fillets supplemented through 0, 20 and 40 days, and their influence on the fatty acid composition and nutritional quality of raw and grilled fillets. A control diet and a diet containing those carotenoids were elaborated with the same lipid source (soybean oil). For carotenoids analysis, the extraction was performed according to the literature, employing acetone and hexane. The relative intensities of m/z ratios of those carotenoids ions were obtained by ultraperformance liquid chromatography-tandem mass spectrometry with chemical ionization at atmospheric pressure (UPLC-APCI MS/MS), and an increase of carotenoid concentration in the supplemented fillets was observed, especially after the grilling process (180 ºC for 15 min). An increase in the concentration of total carotenoids in grilled fillets (75 and 35% higher than raw fillets, for 20 and 40 days of supplementation, respectively, corresponding to 8.32 and 2.42 µg β-carotene g-1 sample) was also observed. Therefore, the carotenoids promoted the stability of lipid oxidation in grilled samples; fact that can be correlated with antioxidant effect of these compounds, it can be also explained by the breakdown of carotenoids physical barrier and the conditions used in the grilling process. Moreover, acceptable n-6/n-3 indices and recommended polyunsaturated fatty acids/saturated fatty acids (PUFA/SFA) values were observed in supplemented fillets. Consequently, lutein, β-carotene and lycopene were considered viable as additive on fish diet, since the supplementation promoted the increase of food quality, even after preparation for consumption.

https://doi.org/10.21577/0103-5053.20180049 563 downloads

15.

Impact of Cafeteria Diet on the Composition of Fatty Acids in Zebrafish (Danio rerio) Fillets
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Néia, Vanessa B. M. J. C.

; Ambrosio-Albuquerque, Eliane P.

; Figueiredo, Ingrid L.

; Boeing, Joana S.

; Silva, Thibério C. da

; Lewandowski, Vanessa

; Ribeiro, Ricardo P.

; Visentainer, Jeane E. L.

; Visentainer, Jesuí V.

Journal of the Brazilian Chemical Society

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Jun 2018, Volume 29 Nº 6 Páginas 1183 - 1188

Resumo: En | Texto: En | PDF: En

The cafeteria diet is widely used as a model for inducing obesity in animals, and the zebrafish (Danio rerio) has emerged as a promising model for this purpose. In this context, this study evaluated the effect of a cafeteria diet on the composition of fatty acids in zebrafish fillets. Zebrafish grown in tanks for a period of 60 days were treated with standard (ST) and cafeteria (CAF) diets. The CAF diet presented higher total lipid and energy values than ST diet, due to the addition of peanut, chocolate and biscuit in its preparation. Moreover, CAF diet showed the highest concentrations of palmitic (19.44 ± 0.55 mg g-1), oleic (102.58 ± 2.85 mg g-1) and linoleic (44.47 ± 1.32 mg g-1) acids. A significant increase in oleic and linoleic acid concentrations (39.77 ± 2.69 and 14.31 ± 0.86 mg g-1, respectively) and in the omega-6/omega-3 ratio (11.52 ± 0.81) was observed in the zebrafish fillets between 0 and 60 days for CAF diet, indicating incorporation of these fatty acids.

https://doi.org/10.21577/0103-5053.20170213 706 downloads

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