ABSTRACT Introduction The college sports environment is characterized by breadth, diversity, and personality. This is an important period to develop students’ physical ability and improve their personality. Objective Compare the effects of different exercise methods on students’ health status. Methods 2991 college students participated in different sports activities. These sports were conducted based on the selection course (PE), all during one semester. The students’ physical health status was observed through experiments performed before and after the intervention. Results Activities such as basketball and soccer showed high effectiveness in improving students’ vital capacity index, volleyball expressively improved students’ performance in the long jump, tennis and table tennis were effective in improving students’ strength and adherence index, being lower in other indices. Martial arts also stood out in improving the students’ vital capacity index. Conclusion Improving physical health should be an overall process of students’ fitness development, and universities should actively encourage college students to participate in long-term sports to improve their health. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción El entorno del deporte universitario se caracteriza por su alcance, diversidad y personalidad. Es un periodo importante para desarrollar la capacidad física de los alumnos y mejorar su personalidad. Objetivo Comparar los efectos de diferentes métodos de ejercicio sobre el estado de salud de los estudiantes. Métodos 2991 estudiantes universitarios participaron en diferentes actividades deportivas. Estos deportes se llevaron a cabo basándose en el curso de selección (PE), todo ello durante un semestre. El estado de salud física de los alumnos se observó mediante experimentos realizados antes y después de la intervención. Resultados Actividades como el baloncesto y el fútbol mostraron una alta eficacia para mejorar el índice de capacidad vital de los alumnos, el voleibol mejoró expresivamente el rendimiento de los alumnos en salto de longitud, el tenis y el tenis de mesa fueron eficaces para mejorar el índice de fuerza y adherencia de los alumnos, siendo inferiores en otros índices. También las artes marciales se destacaron en la mejora del índice de capacidad vital de los alumnos. Conclusión La mejora de la salud física debería ser un proceso global del desarrollo de la forma física de los estudiantes y las universidades deberían animar activamente a los universitarios a participar en deportes de larga duración para mejorar su salud. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução O ambiente do esporte universitário é caracterizado pela abrangência, diversidade e personalidade. Este é um período importante para desenvolver a capacidade física dos estudantes e melhorar a sua personalidade. Objetivo Comparar os efeitos de diferentes métodos de exercício sobre o estado de saúde dos estudantes. Métodos 2991 estudantes universitários participaram de diferentes atividades esportivas. Estes esportes foram conduzidos com base no curso de seleção (PE), todos durante um semestre. O estado de saúde física dos estudantes foi observado através de experimentos executados previa e posteriormente à intervenção. Resultados Atividades como basquetebol e futebol demonstraram alta efetividade para melhorar o índice de capacidade vital dos estudantes, voleibol melhorou expressivamente o desempenho dos alunos no salto em distância, o tênis e o tênis de mesa foram efetivos para aprimorar o índice de força e de adesão dos alunos, sendo inferior noutros índices. Também as artes marciais se destacaram ao melhorar o índice de capacidade vital dos alunos. Conclusão O aprimoramento da saúde física deve ser um processo global de desenvolvimento da aptidão física dos estudantes e as universidades devem encorajar ativamente os estudantes universitários a participar de esportes de longo prazo para melhorar sua saúde. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Purpose: Oxidative stress and apoptosis contribute to the pathological basis of doxorubicin (DOX)-induced cardiotoxicity. Columbianadin (CBN) is one of the main bioactive constituents isolated from the root of Angelica pubescens. Herein, we intended to explore the potential role and molecular basis of CBN in DOX-induced cardiotoxicity. Methods: C57BL/6 mice were subjected to DOX (15 mg/kg/day, i.p.) to generate DOX-induced cardiotoxicity. CBN (10 mg/kg/day, i.p.) was administered for four week following DOX injection. Results: DOX administered markedly dampened cardiac function, increased cardiac injury, excessive reactive oxygen species (ROS) production, and cardiomyocyte loss. These alterations induced by DOX significantly alleviated by CBN treatment. Mechanistically, our results demonstrated that the CBN exerts cardioprotection role against DOX by up-regulating silent information regulator 1 (Sirt1) and decreasing acetylation of forkhead box O1 (FOXO1). Moreover, Sirt1 inhibition with Ex-527 significantly blunt the beneficial effect of CBN on DOX-induced cardiotoxicity, including cardiac dysfunction, ROS, and apoptosis. Conclusion: Collectively, CBN attenuated oxidative stress and cardiomyocyte apoptosis in DOX-induced cardiotoxicity through maintaining Sirt1/FOXO1 signaling pathway. Our results demonstrated that CBN might be used to treat DOX-related cardiotoxicity.

Abstract: Background: It is challenging to make informed decision in genetic counseling. Shared decision-making provides a chance in balancing the information and preferences between counselors and counselees. However, the status and prescriptions of shared decision-making have not been extensively studied in genetic counseling. Aim: To develop an up-to-date literature review of the shared decision-making in genetic counseling, identify knowledge gaps, and provide inspiration and suggestions for the development and practice of genetic counseling. Methods: “Genetic Counseling” and “Shared decision-making” were used to search in PubMed, Web of Science, Embase, Wanfang, CNKI and CBM databases. The search deadline was March 26, 2021. Results: A total of 22 articles were included, and four themes were identified: how people involved in genetic counseling, different stakeholders involved in SDM, multiple facilitators and barriers to SDM and the effectiveness of SDM in genetic counseling. Conclusion: Each participant needs to recognize their boundaries and make the best effort to involve in the shared decision-making. In the future, multinational studies should be considered for bringing shared decision-making to the global scale and well-designed studies are required to explore the long-term impact of shared decision-making in genetic counseling.

Resumen: Antecedentes: Es un reto tomar una decisión informada en el asesoramiento genético. La toma de decisiones compartida ofrece una oportunidad para equilibrar la información y las preferencias entre los asesores y los pacientes. Sin embargo, el estado y las prescripciones de la toma de decisiones compartida no se han estudiado ampliamente en el asesoramiento genético. Objetivo: Desarrollar una revisión bibliográfica actualizada de la toma de decisiones compartida en el asesoramiento genético, identificar las lagunas de conocimiento y proporcionar inspiración y sugerencias para el desarrollo y la práctica del asesoramiento genético. Métodos: “Genetic Counseling” y “Shared decision-making” se utilizaron para buscar en las bases de datos PubMed, Web of Science, Embase, Wanfang, CNKI y CBM. La fecha límite de búsqueda fue el 26 de marzo de 2021. Resultados: Se incluyó un total de 22 artículos y se identificó cuatro temas: cómo se involucran las personas en el asesoramiento genético, las diferentes partes interesadas involucradas en la GDS, los múltiples facilitadores y barreras para la GDS y la efectividad de la GDS en el asesoramiento genético. Conclusión: Cada participante necesita reconocer sus límites y hacer el mejor esfuerzo para involucrarse en la toma de decisiones compartida. En el futuro, se debe considerar la realización de estudios multinacionales para llevar la toma de decisiones compartida a la escala global y se requieren estudios bien diseñados para explorar el impacto a largo plazo de la toma de decisiones compartida en el asesoramiento genético.

Resumo Antecedentes: Tomar uma decisão informada em aconselhamento genético é um desafio. A tomada de decisão compartilhada (SDM) fornece uma oportunidade para contrabalançar a informação e preferências entre conselheiros e aconselhados. Entretanto, o status e prescrições da tomada de decisão compartilhada não têm sido extensivamente estudados em aconselhamento genético. Objetivo: Desenvolver uma revisão de literatura atualizada sobre a tomada de decisão compartilhada em aconselhamento genético, identificar lacunas no conhecimento e fornecer inspiração e sugestões para o desenvolvimento e prática do aconselhamento genético. Métodos: “Genetic Counseling” e “Shared decision-making” foram usados para pesquisar nos bancos de dados PubMed, Web of Science, Embase, Wanfang, CNKI e CBM. A data limite para a pesquisa foi 26 de março de 2021. Resultados: Um total de 22 artigos foram incluídos e quatro temas foram identificados: como pessoas se envolveram em aconselhamento genético, diferentes interessados envolvidos em SDM, facilitadores e barreiras múltiplas à SDM e efetividade da SDM em aconselhamento genético. Conclusão: Cada participante necessita reconhecer seus limites e fazer os melhores esforços para se envolver em tomada de decisão compartilhada. No futuro, estudos multinacionais devem ser considerados para trazer a tomada de decisão compartilhada a uma escala global e estudos bem desenhados são requeridos para explorar o impacto a longo prazo da tomada de decisão compartilhada em aconselhamento genético.

RESUMO Introdução: A fadiga é uma manifestação de um estado de saúde inferior, que causa efeitos negativos graves na vida diária. As propriedades antioxidantes da crocina foram amplamente investigadas em muitas doenças. No entanto, nenhuma correlação entre crocina e fadiga foi relatada. Objetivo: Verificar a eficácia antifadiga da crocina com relação ao tempo de exaustão induzido pela natação. Materiais e métodos: Neste estudo, os camundongos foram tratados com crocina por 28 dias para avaliar sua eficácia antifadiga. Tempo de exaustão, índices bioquímicos relevantes para a fadiga, citocinas inflamatórias, indicadores do metabolismo energético, parâmetros de oxidação e a via NRF-2/HO-1 foram analisados para explorar evidências de crocina em exercícios de alta intensidade. Resultados: Nossa pesquisa indicou que a crocina estendeu notavelmente o tempo induzido pela natação até a exaustão. Em natação extenuante, a crocina eliminou claramente o BLA e o SUN para manter a homeostase do ambiente interno, ao mesmo tempo em que melhorou significativamente as concentrações de glicogênio nos músculos e no fígado para promover as reservas de energia. Além disso, a crocina melhorou visivelmente a reação inflamatória, como mostram as reduções de TNF-a e IL-6, promovendo a resistência. Nos músculos, a crocina aumentou visivelmente as atividades de SDH e Na + K ± ATP para melhorar o metabolismo energético em natação extenuante. Por último, a crocina melhorou muito as atividades de SOD e CAT por meio da via NRF-2/HO-1 quanto à defesa contra a fadiga induzida pelo estresse oxidativo. Conclusões: A crocina proporciona resistência à oxidação e pode ser usada em nutrientes antifadiga. Nível de evidência II; Estudo prospectivo comparativo.

ABSTRACT Introduction: Fatigue is a manifestation of sub-health status, which causes serious negative effects in daily life. The antioxidant properties of crocin have been widely investigated in many diseases. However, no correlation between crocin and fatigue was reported. Objective: To verify the anti-fatigue efficacy of crocin in swimming-induced exhaustive time. Materials and Methods: In this study, mice were treated with crocin for 28 days to assess its anti-fatigue efficacy. Exhaustive time, fatigue-relevant biochemical indices, inflammatory cytokines, energy metabolism indicators, oxidation parameters, and the NRF-2/HO-1 pathway were analyzed to explore evidence of crocin in high-intensity exercise. Results: Our research indicated that crocin dramatically extended swimming-induced exhaustive time. In strenuous swimming, crocin clearly eliminated BLA and SUN to maintain internal environment homeostasis, while it markedly improved glycogen concentrations in the muscles and liver to promote energy reserves. Moreover, crocin visibly improved the inflammatory reaction, as represented by reductions in TNF-a and IL-6, promoting endurance capacity. In the muscles, crocin noticeably enhanced SDH and Na+K+-ATP activities to improve energy metabolism in strenuous swimming. Lastly, crocin markedly improved SOD and CAT activities via the NRF-2/HO-1 pathway to defend against oxidative stress-induced fatigue. Conclusions: Crocin provides oxidation resistance and can be developed into anti-fatigue nutriments. Evidence level II; Comparative prospective study.

RESUMEN Introducción: La fatiga es una manifestación del mal estado de salud, que provoca graves efectos negativos en la vida cotidiana. Las propiedades antioxidantes de la crocina han sido ampliamente investigadas en muchas enfermedades. Sin embargo, no se ha reportado ninguna correlación entre la crocina y la fatiga. Objetivo: Verificar la eficacia antifatiga de la crocina con respecto al tiempo de agotamiento inducido por la natación. Materiales y métodos: En este estudio, se aplicó el tratamiento de crocina a ratones durante 28 días para evaluar su eficacia antifatiga. Se analizaron el tiempo de agotamiento, los índices bioquímicos relevantes para la fatiga, las citocinas inflamatorias, los indicadores del metabolismo energético, los parámetros de oxidación y la vía NRF-2/HO-1 para explorar evidencias de la crocina en ejercicios de alta intensidad. Resultados: Nuestra investigación indicó que la crocina prolongó notablemente el tiempo de agotamiento inducido por la natación. En la natación extenuante, la crocina eliminó claramente el BLA y el SUN para mantener la homeostasis del ambiente interno, al tiempo que aumentó significativamente las concentraciones de glucógeno en el músculo y el hígado para favorecer las reservas de energía. Además, la crocina mejoró visiblemente la reacción inflamatoria, como lo demuestran las reducciones de TNF-a e IL-6, promoviendo la resistencia. En los músculos, la crocina aumentó visiblemente las actividades de SDH y Na + K ± ATP para mejorar el metabolismo energético en la natación extenuante. Por último, aumentó en gran medida las actividades de SOD y CAT a través de la vía NRF-2/HO-1 en la defensa contra la fatiga inducida por el estrés oxidativo. Conclusiones: La crocina proporciona resistencia a la oxidación y puede utilizarse en nutrientes antifatiga.Nivel de evidencia II; Estudio prospectivo comparativo.

Abstract In the framework of corpus linguistics and based on the Corpus of Spanish in the 21st Century and the Contemporary Chinese National Corpus, this paper takes Spanish adverb of degree demasiado (“too”, in english) and its Chinese equivalent 太(tài) as example and analyses their semantic prosody with the method proposed by Zhao and Li (2010) and using the software AntConc; at the same time, it explores its teaching application. The conclusions of the study are as follows: 1. Both demasiado and 太(tài) have mixed semantic prosody and their uses frequently express a negative or neutral attitude; 2. Same as 太(tài), demasiado is used as a kind of euphemism in negative sentences; 3. Compared to 太(tài), demasiado expresses the positive attitude less; 4. In the process of teaching Spanish to Chinese speakers, assisted by the study of semantic prosody we can deepen the semantic analysis of words, visualize their most common combinations and avoid the negative transfer of Chinese.

Resumen En el marco de la lingüística de corpus y con base en el Corpus del Español del Siglo xxi y el Corpus Nacional de Chino Contemporáneo, con el método propuesto por Zhao y Li (2010) y analizado mediante el programa AntConc, este trabajo realiza un análisis de la prosodia semántica con el caso ejemplificado del adverbio de grado español demasiado y su equivalente en chino 太(tài), asimismo, indaga en su aplicación didáctica. Las conclusiones del estudio son las siguientes: 1. Tanto demasiado como 太(tài) cuentan con una prosodia semántica mixta y sus usos frecuentemente expresan una actitud negativa o neutral; 2. Al igual que太(tài), demasiado es usado como un tipo de eufemismo en oraciones negativas; 3. Demasiado en comparación con 太(tài) expresa en menos ocasiones una actitud positiva; 4. Al impartir el español a sinohablantes, el estudio de la prosodia semántica puede contribuir a la profundización del análisis semántico de las palabras, visualizar sus combinaciones más frecuentes y evitar la transferencia negativa del chino.

Abstract Objective To explore the molecular mechanism of neuropathologic damage induced by radiofrequency ablation at different temperatures. Methods This is basic research, and 36 SD rats were used to construct the neuropathological injury model. The rats were subjected to radiofrequency stimulation at different temperatures and were divided into 6 groups according to the temperature injury: 42°, 47°, 52°, 57°, 62°, and 67°C groups. Conduction time, conduction distance, and nerve conduction velocity were recorded after temperature injury. HE-staining was used to observe the histopathological morphology of the sciatic nerve. The expression of SCN9A, SCN3B, and NFASC protein in sciatic nerve tissue were detected by western blot. Results With the increase in temperature, nerve conduction velocity gradually decreased, and neurons were damaged when the temperature was 67°C. HE-staining showed that the degrees of degeneration of neurons in rats at 47°, 52°, 57°, 62°, and 67°C were gradually increased. The expression of SCN9A, SCN3B protein in 57°, 62°, 67°C groups were much higher than that of NC, 42°, 47°, 52°C groups. However, the expression of NFASC protein in 57°, 62°, 67°C groups was much lower than that of the NC, 42°, 47°, 52°C groups. Conclusion There was a positive correlation between temperature caused by the radiofrequency stimulation to neuropathological damage. The mechanism is closely related to the expression of SCN9A, SCN3B, and NFASC protein in nerve tissue caused by heat transfer injury.

Abstract In order to realize the rapid nondestructive detection of mildew peanut in the process of peanuts storage, hyperspectral imaging technology was proposed to detect mildew peanut. A total of 200 peanuts were selected from 5 kinds of peanuts purchased in the market for moldy treatment, and the remaining 400 peanuts were kept sterile. After completion, samples were collected with a hyperspectral instrument to obtain spectral data of the samples. According to the characteristics of the data, 10 pre-processing algorithms were used to de-noise the data, and Median Filtering (MF) had the best effect, with the recognition accuracy reaching 97.7%. GBDT, LightGBM, CatBoost and XGBoost algorithms were used to extract important feature bands in the spectral data pre-processed by MF. GBDT, LightGBM, CatBoost and XGBoost algorithms were used to model the extracted feature bands. The results showed that LightGBM is the best algorithm with a detection rate of 99.10%. Optuna algorithm was used to tune its parameters. Compared with the previous model, the running time of the optimized model was improved by about 0.25 s. The results showed that hyperspectral imaging provides an efficient and nondestructive method for detecting mildew in peanut storage.

Although metastasis is the major cause of death in cervical cancer, the mechanism of metastasis is still unclear. The mRNA expression and protein level of latent transforming growth factor beta binding protein 1 (LTBP1) were detected in tumor tissues and paracancerous tissues from in-house samples. Cell proliferation, cell cycle, migration, and in vivo metastasis were determined after LTBP1 was knocked down. Then, 13 drugs were screened, and the changes in cell apoptosis and proliferation and tumor metastasis were detected after drug treatment in shRNA cells. In our in-house samples, LTBP1 was lowly expressed in cervical cancer tissues. After LTBP1 knockdown, cell proliferation was increased, and the ability of in vitro migration and in vivo metastasis was enhanced. At the same time, the proportion of myeloid derived suppressor cells (MDSC) in situ increased, the proportion of T cells decreased, and transforming growth factor beta-1 (TGFβ1) signaling was activated. After carboplatin treatment, LTBP1 shRNA cell line apoptosis increased, metastasis in vivo was limited, and the proportion of MDSC in situ decreased. LTBP1 was lowly expressed in cervical cancer, and the inhibition of LTBP1 can improve the malignant degree of the tumor, and this process can be blocked by carboplatin.

RESUMO Para explorar o Impacto Da formação funcional Na qualidade de conclusão Da dificuldade do corpo, OS ginastas rítmicos 24.o com Dez Anos de idade 10-11 foram aleatoriamente divididos EM Grupo de formação funcional Ou Grupo de controlo e EM formação contínua 2h/dia para um mês.A dificuldade do corpo, incluindo dificuldades de equilíbrio, dificuldades de rotação, dificuldades de Salto foram medidas antes e depois Da formação.Os resultados mostraram pontuação total, pontuação Da rotação de Passos de 360°e Salto Split no Grupo de treinamento funcional FOI respectivamente 1.87, 0.77 e 0.42 Pontos Mais elevados do que aqueles no Grupo de controle (todos P<0.05) após o treinamento de um mês. Por conseguinte, Pode concluir-se que a formação funcional baseada no SGF Pode obviamente melhorar a qualidade de conclusão Da dificuldade corporal Da ginástica rítmica juvenil num curto período de tempo.

ABSTRACT To explore the impact of functional training on completion quality of body difficulty, 24 rhythmic gymnasts aged 10-11 years old were randomly and averagely divided into functional training and control group and trained 2h/day for 1 month. Body difficulty – including balance, rotation, and leap difficulties – was measured before and after training. The results showed that the total score, the score of 360° passé pivot and the score of split leap for the functional training group was respectively 1.87, 0.77, and 0.42 points higher than those for the control group (P<0.05) after 1 month of training. Therefore, it can be concluded that functional training based on Functional Movement Screen (FMS) can clearly improve completion quality of body difficulty in juvenile rhythmic gymnastics within a short period of time.

RESUMEN Los resultados mostraron que el grupo de control y el grupo de gimnasia rítmica de 10 a 11 años de edad fueron de dos horas de entrenamiento al día durante un mes. Las dificultades físicas, incluidas las dificultades de equilibrio, rotación y salto, se miden antes y después del entrenamiento. Los resultados mostraron que la puntuación total, la puntuación de 360°passe pivot y la puntuación de split-jump fueron respectivamente 1,87, 0,77 y 0,42 mayores en el grupo de entrenamiento funcional que en el grupo control después de 1 mes de entrenamiento (P< 0,05). Los resultados muestran que el entrenamiento funcional basado en la detección de movimiento funcional (FMS) puede mejorar significativamente la calidad de la realización de las dificultades físicas en la gimnasia rítmica de los adolescentes en un corto período de tiempo.

Abstract In this study, we investigated the preconsolidation pressure (P C) of sedimentary soft rock and its relationship with brittle-ductile transition (BDT) pressure. We proposed a generalized preconsolidation pressure (P G) suitable for both soil and sedimentary soft rock, which is defined in terms of the yield stress of the initial sediment structure under confined lateral compression. We then explored the digenetic effect on P G using 24 sediment types. Next, P G and BDT pressure were verified using confined compression and conventional triaxial tests, respectively, on red-bed soft rock in the Dingxi region of China. Finally, we discussed the BDT mechanism and analyzed the relationship between the two characteristic pressures for a given initial yield surface considered structural strength. We found that the relationship was dependent on the strength parameters, the initial structural strength, and the coefficient of earth pressure at rest. The findings of this study will have a significant impact on the determination of BDT pressure of sedimentary rock in future studies.

Given their relationship with metabolic syndrome and systematic inflammatory diseases, the pathogenesis of hypertension, hyperglycemia, and hyperlipidemia is closely related. To explore the common genes among these three conditions, spontaneous hypertensive rats (SHR), spontaneous diabetic Goto-Kakizaki rats (GK) and hyperlipidemia rats (HMR) were reared for experiments. Gene array was used to identify the genes of SHR, GK and HMR compared with normal Wistar rats using TBtools software. First, real-time PCR was applied to verify these genes, and Cytoscape software was used to construct networks based on the National Center for Biotechnology Information (NCBI) database. Second, Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis was performed to classify the genes. Visualization and Integrated Discovery (DAVID) database and Gene Ontology database were used to explore the biological function. Finally, Onto-tools Pathway Express was used to analyze the pathways of shared genes. Importantly, upregulated common genes, such as Bad, Orm1, Arntl and Zbtb7a, were used to construct a network of 150 genes, while downregulated genes, such as Mif and Gpx1, formed a network of 29 genes. Interestingly, the networks were involved in various pathways, such as insulin signal pathway, endometrial cancer pathway, circadian rhythm pathway, and pancreatic cancer pathway. We discovered common genes of SHR, GK and HMR compared with normal Wistar rats, and the association of these genes together with biological function were preliminarily revealed.

SUMMARY: The aim of this study was to evaluate the effect of soft tissue thickness of upper lip on lip retraction in orthodonticaltreated females and identify the ratio of maxillary incisor retraction to upper lip retraction. Pre- and post-treatment lateral cephalograms of 100 adults were examined to measure the lip thickness in upper lip and establish the classification standard. All subjects were treated with 4 first premolar extractions followed by upper central incisors retraction. Pre- and post-treatment lateral cephalograms of 19 patients were reviewed to determine the changes of the upper lip and incisor positions through landmarks displacement. An independent-samples t test and one-way analysis of variance were performed. The correlations between maxillary incisor retraction and upper lip retraction were explored by the Pearson correlation method. P-values<0.05 were considered statistically significant. The lip thickness of adult male patients was greater than that of adult female patients. The average ratio of maxillary incisor retraction to upper lip repositioning was 1.6:1,1.9:1 and 2.2:1 in the thin lips group, normal lips group and thick lips group, respectively. Gender differences exist in the thickness of upper lip. Horizontal changes of the maxillary incisor showed a significant correlation to horizontal changes of the upper lip (P<0.001).There were negative correlations between the thickness of upper lip and the ratio between change in maxillary incisor protrusion and change in upper lip protrusion.

RESUMEN: El objetivo de este estudio fue evaluar el efecto del grosor de los tejidos blandos del labio superior, sobre la retracción del labio en mujeres tratadas con ortodoncia e identificar la proporción de retracción del diente incisivo maxilar con respecto a la retracción del labio superior. Se examinaron cefalogramas laterales, previos y posteriores al tratamiento de 100 adultos, para medir el grosor del labio superior y establecer un estándar de clasificación. Todos los sujetos fueron tratados con 4 extracciones de los primeros premolares seguidas de retracción de los incisivos centrales superiores. Se revisaron los cefalogramas laterales, previos y posteriores al tratamiento de 19 pacientes, para determinar los cambios del labio superior y las posiciones de los incisivos a través del desplazamiento de los puntos de referencia. Se realizó una prueba t de muestras independientes y un análisis de varianza de una vía. Las correlaciones entre la retracción del incisivo maxilar y la retracción del labio superior se exploraron mediante el método de correlación de Pearson. Los valores de p<0,05 fueron considerados estadísticamente significativos. El grosor de los labios de los pacientes adultos masculinos fue mayor que el de las pacientes adultas. La relación promedio de la retracción del incisivo maxilar al reposicionamiento del labio superior fue de 1,6:1,1; 9:1 y 2,2:1 en el grupo de labios delgados, grupo de labios normales y grupo de labios gruesos, respectivamente. Existen diferencias de sexo en el grosor del labio superior. Los cambios horizontales del incisivo maxilar mostraron una correlación significativa con los cambios horizontales del labio superior (P<0,001). Hubo correlaciones negativas entre el grosor del labio superior y la relación entre el cambio en la protuberancia del incisivo maxilar y el cambio en la protrusión del labio superior.

Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal antibodies and a polyclonal antibody to regucalcin were produced, a double-antibody sandwich ELISA method was established, and serum regucalcin was determined in 47 chronic hepatitis B (CHB) patients, 91 HBV-related acute-on-chronic liver failure (HBV-ACLF) patients, and 33 healthy controls. The ELISA demonstrated an appropriate linear range, and high levels of reproducibility, sensitivity, specificity, accuracy, and stability. The median serum regucalcin concentrations in HBV-ACLF and CHB patients were 5.46 and 3.76 ng/mL, respectively (P<0.01), which were much higher than in healthy controls (1.72 ng/mL, both P<0.01). For the differentiation of CHB patients and healthy controls, the area under curve (AUC) was 0.86 with a cut-off of 2.42 ng/mL, 85.7% sensitivity, and 78.8% specificity. In contrast, the AUC of alanine aminotransferase (ALT) was lower (AUC=0.80, P=0.01). To differentiate ACLF from CHB, the AUC was 0.72 with a cut-off of 4.26 ng/mL, 77.0% sensitivity, and 61.2% specificity while the AUC of ALT was 0.41 (P=0.07). Thus, we have developed an ELISA that is suitable for measuring serum regucalcin and have shown that serum regucalcin increased with the severity of liver injury due to HBV-related diseases, such that it appears to be more useful than ALT as a marker of liver injury.

Previous studies suggested that chromodomain helicase DNA-binding proteins (CHDs), including CHD 1–8, were associated with several human diseases and cancers including lymphoma, liver cancer, colorectal cancer, stomach cancer, etc. To date, little research on CHD 9 in human cancers has been reported. In this study, we assessed the prognostic value of CHD 9 in patients with colorectal cancer (CRC). We screened for CHD 9 expression using immunohistochemical analysis in 87 surgical CRC specimens and found that the expression was upregulated in 81.5% of the cases, while 7.4% were decreased; in the remaining 11.1% of the cases, levels were not altered. Kaplan-Meier analysis showed that patients with high CHD 9 expression had better prognosis than those with low CHD 9 expression (54.5 vs 32.1%, P=0.034). Subsequently, Cox multi-factor survival regression analysis revealed that expression of CHD 9 protein was an independent predictor for CRC, with a hazard ratio of 0.503 (P=0.028). In addition, we found that CHD 9 expression was positively correlated with MSH2 (rs=0.232, P=0.036). We speculated that CHD9 might be a putative tumor suppressor gene, and could inhibit the development of CRC by participating in DNA repair processes. Our findings suggest that CHD 9 could be a novel prognostic biomarker and a therapeutic target for CRC. Further studies are needed to detect the effect of CHD 9 on cellular function and the expression of mismatch repair genes.

The mortality of patients with mycotic aneurysms is high, especially in East Asia, and infection by Salmonella species is the most common. Our study aimed to improve prognosis of adult mycotic aneurysms with early diagnosis and accurate treatment. Four adult patients with mycotic aneurysm caused by Salmonella were included and analyzed by single-center retrospective analysis. Cases reported in the literature during the past 10 years were also summarized. The average age of the 4 male patients was 61.25 years, while that of the 53 cases reported in the literature was 65.13 years. Hypertension, diabetes, and atherosclerosis were common complications. Most patients presented fever and experienced pain at the corresponding position of the aneurysm. Laboratory examination found an increased number of white blood cells accompanied by an increase in inflammatory markers. Most aneurysms were found in the abdominal aorta, while the rupture of an aneurysm was the most common complication. The mortality rates were 21.43 and 7.14% after open surgery or endovascular aneurysm repair (EVAR) intervention, respectively. The recurrence rates of infection were 0 and 17.85% for both treatments, respectively. The mortality rate of mycotic aneurysm caused by Salmonella infection was high in middle-aged males with hypertension, diabetes, and atherosclerosis. The possibility of a Salmonella-infected aneurysm should be considered in these high-risk groups presenting chills, fever, chest, and back pain. Open surgery was superior to EVAR treatment in the clearance of infected foci and the reduction of postoperative recurrence. The recurrence of postoperative infection can be prevented by intravenous antibiotic therapy for 6 weeks post-surgery.