Pesquisa | SciELO

ABSTRACT: Lettuce and arugula are the most cultivated leafy vegetables in hydroponic systems in the world, and the little information about Azospirillum brasilense effects in research in this system makes it necessary to verify the best method of inoculation of these bacteria. Therefore, this study aimed to verify the beneficial effects of inoculation methods of A. brasilense on plant growth and nutrition of lettuce and arugula grown in the hydroponic system. The study was conducted in a greenhouse with 30% shading. A randomized block design with five replicates in a 4 × 2 factorial scheme was used. Four A. brasilense inoculation methods (non-inoculated, foliar application, nutrient solution, and nutrient solution + foliar application) and two leafy vegetable species - arugula and lettuce - were evaluated. All inoculation methods improved plant height, shoot fresh and dry mass, root fresh and dry mass, fresh leaf yield, leaf chlorophyll index, accumulation of nitrogen, potassium, calcium, sulfur, phosphorus, iron, manganese, and copper and reduced nitrate accumulation in shoot and root of arugula and lettuce. Inoculation via nutrient solution + foliar application of A. brasilense is the most suitable for hydroponic arugula cultivation because it provides greater fresh leaf yield. All inoculation methods increased leaf yield in hydroponic lettuce. Hydroponic arugula and lettuce plants showed greater accumulation of nutrients and plant growth with less nitrate accumulation in the shoot under inoculation with A. brasilense.

RESUMO A alface e a rúcula são as hortaliças folhosas mais cultivadas em sistemas hidropônicos no mundo, e as poucas informações sobre os efeitos do Azospirillum brasilense em pesquisas neste sistema torna-se necessário verificar o melhor método de inoculação dessas bactérias. Portanto, o objetivo deste estudo foi verificar os efeitos benéficos de métodos de inoculação de A. brasilense em alface e rúcula no crescimento e nutrição das plantas em sistema hidropônico. O estudo foi conduzido em casa de vegetação com 30% de sombreamento. Foi usado o delineamento em blocos ao acaso com 5 repetições em esquema fatorial 4 × 2, com quatro métodos de inoculação de A. brasilense (não inoculado, foliar, solução nutritiva e solução nutritiva + inoculação foliar) e o segundo fator foi a aplicação em rúcula e alface. Todos os métodos de inoculação melhoraram a altura da planta, massa fresca e seca da parte aérea, massa fresca e seca da raiz, rendimento da planta, índice de clorofila foliar, acúmulo de nitrogênio, potássio, cálcio, enxofre, fósforo, ferro, manganês, cobre e redução do acúmulo de nitrato na parte aérea e raízes de rúcula e alface. A inoculação via solução nutriente + inoculação foliar de A. brasilense é a mais indicada para o cultivo hidropônico de rúcula, pois proporciona maior rendimento de folhas, e todos os métodos de inoculação foram capazes de aumentar o rendimento de folhas em alface hidropônica. Plantas hidropônicas de rúcula e alface apresentaram maior acúmulo de nutrientes e crescimento das plantas com menor acúmulo de nitrato na parte aérea sob inoculação com A. brasilense.

https://doi.org/10.1590/1807-1929/agriambi.v27n9p653-662

Seasonal and Circadian Evaluation of the Pectis brevipedunculata Essential Oil and Its Acaricidal Activity against Rhipicephalus microplus (Acari: Ixodidae)
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Camara, Marcos B. P.

; Lima, Aldilene S.

; Jumbo, Luis Oswaldo V.

; Tavares, Caio P.

; Mendonça, Cáritas de Jesus S.

; Monteiro, Odair S.

; Araújo, Sabrina Helena C.

; Oliveira, Eugênio Eduardo de

; Lima Neto, José S.

; Maia, José Guilherme S.

; Costa Junior, Livio M.

; Rocha, Cláudia Q. da

Journal of the Brazilian Chemical Society

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Jul 2023, Volume 34 Nº 7 Páginas 1020 - 1029

Resumo: En | Texto: En | PDF: En

Pectis brevipedunculata is native species and widely available in dry and semi-arid ecosystems showing high biotechnological potential. The objective of this study was to evaluate the circadian and seasonal chemical variation of the essential oil (EO) of P. brevipedunculata, as well as its acaricide effect on Rhipicephalus microplus larvae. Aerial parts were collected and submitted to the hydrodistillation process, and the chemical composition was determined by gas chromatography mass spectrometry (GC-MS). For the assays with R. microplus, the larval immersion test was performed. The main constituents were citral (75% of the EO), followed by α-pinene and limonene. In the seasonality analysis, the highest yields were in the months of April (2.08%) and August (2.05%), while in the study of circadian rhythm, the percentage was 2.0% at 6 p.m. in the rainy season, and 1.2%, dry season at 6 p.m. Concerning acaricidal activity (50% lethal concentration (LC50)), the April (1.17 mg mL-1), March (1.28 mg mL-1), June (1.37 mg mL-1), and October (1.27 mg mL-1) oils obtained were the most active and assays performed with circadian rhythm revealed in the rain season (April) at 6 p.m. and dry season (September) at 12 a.m. LC50 values of 1.75 and 1.75 mg mL-1, respectively. Additionally, this EO is selective to non-target organisms, i.e., ladybeetles and lacewing.

https://doi.org/10.21577/0103-5053.20230017

Detection of Cutibacterium acnes in Tissue Samples from Clean Primary Shoulder Surgeries – Part II
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Miyazaki, Alberto Naoki

; Salles, Mauro José Costa

; Gonçalves, Guilherme Vieira

; Conte, Luiz Henrique Gallego

; Oliveira, Thomaz Gê de

; Santili, Ana Beatriz Nappi

; Kurihara, Mariana Neri Lucas

; Santos, Ingrid Nayara Marcelino

; Silva, Luciana Andrade da

Revista Brasileira de Ortopedia

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Abr 2023, Volume 58 Nº 2 Páginas 257 - 264

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Objective Research and identification of Cutibacterium acnes (C. acnes) and other microorganisms in deeptissue samples collected in clean shoulder surgeries of patients who did not undergo any previous invasive joint procedure and who had no clinical history of infection. Methods We analyzed the results of cultures of intraoperative deep tissue samples from 84 patients submitted to primary clean shoulder surgery. Tubes containing culture medium were used for storage and transport of anaerobic agents, prolonged incubation time, and mass spectrometer for diagnosis of bacterial agents. Results Bacteria growth was evidenced in 34 patients (40.4%) of the 84 included in the study. Of these, 23 had growth of C. acnes in at least one sample of deep tissue collected, corresponding to 27.3% of the total patients. The second most common agent was Staphylococcus epidermidis, present in 7.2% of the total individuals included. We showed a higher relationship between sample positivity and males, a lower mean age, absence of diabetes mellitus, ASA I score, and antibiotic prophylaxis in anesthetic induction with cefuroxime. Conclusions A high percentage of isolates of different bacteria was found in shoulder tissue samples of patients undergoing clean and primary surgeries, who had no history of previous infection. Identification of C. acnes was high (27.6%), and Staphylococcus epidermidis was the second most frequent agent (7.2%).

Resumo Objetivo Pesquisa e identificação de Cutibacterium acnes (C. acnes) e de outros microrganismos em amostras de tecidos profundos coletados em cirurgias limpas de ombro em pacientes que não foram submetidos a nenhum procedimento invasivo articular prévio e que não possuíam antecedentes clínicos de infecção. Métodos Foram analisados os resultados das culturas de amostras de tecidos profundos intraoperatórias de 84 pacientes submetidos à cirurgia limpa primária do ombro. Foram utilizados tubos contendo meio de cultivo para armazenamento e transporte de agentes anaeróbicos, tempo prolongado de incubação e espectrômetro de massa para diagnósticos de agentes bacterianos. Resultados Foi evidenciado o crescimento de bactérias em 34 pacientes (40,4%) dos 84 incluídos no estudo. Desses, 23 apresentavam crescimento de C. acnes em pelo menos uma amostra de tecido profundo coletada, correspondendo a 27,3% do total de pacientes. O segundo agente mais encontrado foi o Staphylococcus epidermidis, presente em 7,2% do total de indivíduos incluídos. Evidenciamos maior relação da positividade de amostras com o gênero masculino, uma média de idade inferior, a ausência de diabetes mellitus, o escore ASA I e a profilaxia antibiótica na indução anestésica com cefuroxima. Conclusões Verificou-se um elevado percentual de isolados de diferentes bactérias em amostras de tecidos de ombros de pacientes submetidos a cirurgias limpas e primárias e sem histórico de infecção anterior. A identificação de C. acnes foi elevada (27,6%) e o Staphylococcus epidermidis foi o segundo agente mais frequente (7,2%).

https://doi.org/10.1055/s-0042-1757306

Phenolic Compounds from Leaves of Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae): A Brazilian Atlantic Forest Tree
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Tiepo, Angelica N.

; Coutinho, Isabel D.

; Machado, Guilherme O.

; Oliveira, Halley C.

; Pimenta, José Antonio

; Henning, Liliane Marcia M.

; Colnago, Luiz Alberto

; Stolf-Moreira, Renata

Journal of the Brazilian Chemical Society

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Jan 2023, Volume 34 Nº 1 Páginas 146 - 152

Resumo: En | Texto: En | PDF: En

Cariniana estrellensis (Raddi) Kuntze seedlings have been used in forest restoration programs in the Atlantic Forest biome. However, despite the economic and ecological relevance of this species, to date no general method for rapid identification of primary and secondary metabolites of C. estrellensis leaves has been reported. In this work, we explore the feasibility of using rapid analysis by nuclear magnetic resonance techniques and liquid chromatography coupled to diode array, charged aerosol, and mass detection. The main secondary metabolites identified were hydrolysable tannins, quinic acid, hydroxycinnamic acids (trans-p-coumaric acid, cis p coumaric acid, and trans-ferulic acid), and flavonoids (kaempferol and quercetin derivatives). These compounds are particularly useful as chemotaxonomic markers for the genus Cariniana and for the family Lecythidaceae, and may also be important for pharmacological uses and for the survival of the seedlings in reforestation programs.

https://doi.org/10.21577/0103-5053.20220083

Physical, chemical and sensory implications of pequi (Caryocar brasiliense Camb.) sweet bread made with flour, pulp and fruit by-product
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CUNHA, MARIANA C. DA

; TERRA, LAILA H.

; CAMPOS E SOUSA, PIÊTRA

; VILELA, DAIANA R.

; OLIVEIRA, ANA LÁZARA

; SILVA, JÉSSYCA S.

; SIMÃO, SÉRGIO DOMINGOS

; PEREIRA, JOELMA

; ALVES, JOSÉ GUILHERME L.F.

; CARVALHO, ELISÂNGELA ELENA N. DE

; VILAS BOAS, EDUARDO V.B.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Nº 2 elocation e20201550

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to develop and evaluate bread with the use of pulp and flours of pequi, in partial replacement of water and wheat flour, to develop a bakery product with good technological, nutritional and sensorial qualities. The pequi husk and pulp flours were obtained by means of a thermal pre-treatment, oven drying and standardization of the dry material. Whereas, the bread formulation was defined through the baker’s formulation. Besides, the dehydration process caused significant changes (p<0.05) in the L* value and chromaticity (C*), mainly of the flours (husk and pequi pulp), such changes are due to non-enzymatic oxidative processes and pigment degradation, especially carotenoids. The effect of the substitution of ingredients (wheat flour and water) by husk and pulp flours and pequi pulp contributed to the increase in lipid, crude fiber, nitrogen-free extract and energy value content. However, the substitution promoted changes in the attributes of color and textural properties, such as increased hardness, chewiness and cohesiveness. Nevertheless, all formulations showed good sensory acceptance and thus, pequi sweet breads can be implemented in school meals for contributing and meeting the nutritional recommendations established by the School Feeding Brazilian Program (PNAE).

https://doi.org/10.1590/0001-3765202320201550

Healthcare workers exposed to COVID-19 patients present an inflammatory status and Th2/Th17/Th22 immune profile: findings from before vaccine application in Brazil
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AQUINO, RODRIGO CESAR A. DE

; BARROS, BÁRBARA RAFAELA S.

; SILVA, GUILHERME ANTONIO S.

; SOUSA, GEORON F. DE

; SOUZA, EDSON B. DE

; SILVA, DYEGO R.C.

; NASCIMENTO, ARIONE V. DO

; SÁ, IGOR W.A. DE

; LIMA, ELKER L.S. DE

; SILVA, BÁRBARA O.

; LIMA, LUÍSA P.O. DE

; VIEIRA, AMANDA M.

; BARBOSA NETO, ADAUTO G.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; OLIVEIRA, TALITA HELENA ARAÚJO DE

; CARVALHO, BRUNO M.

; MUNIZ, MARIA TEREZA C.

; FREITAS, ANTONIO CARLOS DE

; CAMPELO JÚNIOR, EVÔNIO B.

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20220502

Resumo: En | Texto: En | PDF: En

Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

β-Cyclodextrins alter the energy metabolism-related enzyme activities in rats
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IANISKI, FRANCINE R.

; OLIVEIRA, AMANDA L. DE

; MEZZOMO, NATHANA JAMILLE

; FRANCESCHI, ITIANE D. DE

; CARMO, GUILHERME M. DO

; CREMONESE, CAMILA R.

; BALDISSERA, MATHEUS D.

; ZANON, JENIFER P.

; KOLLING, JENIFER

; FRIEDERICH, JORDANA DANIELA

; SILVA, IVANA Z.

; GIONGO, JANICE L.

; FEKSA, LUCIANE R.

; VAUCHER, RODRIGO A.

; WANNMACHER, CLOVIS MILTON D.

; RECH, VIRGINIA C.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Nº 2 elocation e20201783

Resumo: En | Texto: En | PDF: En

Abstract Although widely used in medicine, separation technology, and other fields, the effects of cyclodextrins on the activities of phosphoryl transfer enzymes have not been previously evaluated. In vivo studies evaluated the function of cyclodextrins as active compounds. Despite the use of cyclodextrins as active compounds, the effects of cyclodextrins on hepatic and renal tissues remain to be fully elucidated. The primary objective of this study was to evaluate the effects of β- cyclodextrins, methyl-β-cyclodextrin (M-β- cyclodextrins), and (2-hydroxypropyl)-β-cyclodextrin (HP-β-cyclodextrins) on enzyme activities regulating the maintenance of energy homeostasis in the kidney and liver tissues in relation to toxicity. Serum levels of liver and kidney markers were measured, and oxidative stress parameters were assessed. After 60-day treatments, we observed that the administration of β-cyclodextrins and M-β-cyclodextrins inhibited the hepatic activity of pyruvate kinase, an irreversible enzyme within the glycolytic pathway. Additionally, administration of HP-β-cyclodextrins inhibited creatine kinase activity and increased the total sulfhydryl content in kidneys. Here, we demonstrated for the first time that β-cyclodextrins, M-β-cyclodextrins, and HP-β-cyclodextrins cause bioenergetic dysfunction in renal and hepatic tissues. These findings suggest that understanding the balance between cyclodextrins’ efficacy and adverse effects is essential for better accepting their use in medicine.

https://doi.org/10.1590/0001-3765202320201783

The Brazilian Atlantic Bushmaster Lachesis (Linnaeus, 1766) Mitogenome With Insights On Snake Evolution And Divergence (Serpentes: Viperidae: Crotalinae)
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HAMDAN, BRENO

; SEIXAS, VICTOR C.

; NUNES, GISELE L.

; OLIVEIRA, GUILHERME

; BONATTO, SANDRO L.

; VIDAL, AMANDA

; PIRES, EDER S.

; ZINGALI, RUSSOLINA B.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Nº 3 elocation e20220973

Resumo: En | Texto: En | PDF: En

Abstract This study presents the first complete mitogenome of the Brazilian Atlantic bushmaster Lachesis with insights into snake evolution. The total length was 17,177 bp, consisting of 13 PCGs, 22 tRNAs, two rRNAs and a duplicate control region (CRs). Almost all genes were encoded by the heavy-strand, except for the ND6 gene and eight tRNAs (tRNA-Gln, Ala, Asn, Cys, Tyr, Ser[TGA anticodon], Glu, Pro). Only ATG, ATA, and ATC were starting codons for protein-coding sequences. Stop codons mainly were TAA, AGA, AGG, and TAG; whereas ND1, ND3, and CYTB terminated with incomplete stop codons. Phylogeny retrieved Lachesis within the Crotalinae as the sister group of Agkistrodon; and the Lachesis+Agkistrodon clade as the sister group of (Sistrurus+Crotalus)+Bothrops. The tree supports Crotalinae, Viperinae, and Azemiopinae in the Viperidae family, being sister taxa of Colubridae+(Elapidae+Psammophiidae). The mean genetic distance across 15 snake families and 57 nucleotide sequences was 0.37. The overall mean value of genetic distance across the Crotalinae was 0.23, with Lachesis muta exhibiting the shortest distance of 0.2 with Agkistrodon piscivorus, Protobothrops dabieshanensis and P. flavoviridis and the greatest 0.25 with Gloydius blomhoffii, Trimeresurus albolabris, S. miliarius, and Deinagkistrodon acutus. The complete Atlantic L. muta mitogenome presented herein is only the third annotated mitogenome from more than 430 described Brazilian snake species.

https://doi.org/10.1590/0001-3765202320220973

VALIDATION AND RELIABILITY BETWEEN EXTERNAL LOAD ANALYSIS DEVICES FOR SOCCER PLAYERS
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Oliveira Júnior, Otaviano

; Chiari, Roberto

; Lopes, Wisley R.T.

; Abreu, Karoline C.

; Lopes, Augusto D.

; Fialho, Guilherme

; Lasmar, Rodrigo C. P.

; Bittencourt, Natalia F. N.

; Leopoldino, Amanda A. O.

Revista Brasileira de Medicina do Esporte

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Ago 2022, Volume 28 Nº 4 Páginas 286 - 290

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: Testar a confiabilidade entre dois instrumentos com diferentes mecanismos de análise, seja por GPS (modelo GPSPORTS®) ou por análise de vídeo (InStat For Players®), relacionando os resultados de distância total percorrida e distância em alta velocidade ≥ 20km/h (Distância de Corrida de Intensidade Muito Alta, VHIR) durante partidas oficiais de futebol. Desenho do Estudo: Este é um estudo metodológico. Dados de 35 atletas de futebol profissional do sexo masculino, de todas as posições táticas foram incluídos. Idade 29,2 (± 4,8 anos) e gordura corporal 9,9 (± 1,7%), excluindo goleiros (102 análises individuais) foram coletados em jogos oficiais. Na análise dos dados, foram utilizados procedimentos de estatística descritiva para caracterizar a amostra e o coeficiente de correlação intraclasse (ICC) foi utilizado para verificar a concordância quanto à estabilidade e consistência interna dos testes com intervalos de confiança (IC) de 95%. Resultados: O ICC no caso da variável distância total percorrida foi significativo 0,914 (0,876; 0,941) e indicou concordância muito elevada, com o coeficiente de correlação linear indicando forte correlação positiva (p <0,001). O ICC para a variável VHIR não foi significativo, embora o coeficiente de correlação linear indique uma correlação positiva forte (p <0,001). Declaração de Relevância Clínica: Este estudo revela que há boa concordância na comparação de dois sistemas projetados para analisar as demandas de movimento de cada atleta profissional de futebol em relação à distância total percorrida. Nível de evidência I; Estudo metodológico - Investigação de um teste diagnóstico.

ABSTRACT Objective: To test the reliability between two instruments with different analysis mechanisms, either by GPS (model GPSPORTS®) or by video analysis (InStat For Players®), relating the results of total distance covered and distance at high speed ≥ 20km/h (Very High-Intensity Running Distance, VHIR) during official soccer matches. Study Design: This is a methodological study. Data from 35 male professional soccer athletes from all tactical positions were included. Age 29.2 (± 4.8 years) and body fat 9.9 (± 1.7%), excluding goalkeepers (102 individual analyzes) were collected in official matches. In the data analysis, descriptive statistics procedures were used to characterize the sample and the intraclass correlation coefficient (ICC) was used to verify the agreement on the stability and internal consistency of the tests with 95% confidence intervals (CI). Results: The ICC in the case of the total distance traveled variable was significant 0,914 (0,876; 0,941) and indicated a very high agreement, with the linear correlation coefficient indicating a strong positive correlation (p <0.001). The ICC for the VHIR variable was not significant, although the linear correlation coefficient indicates a strong positive correlation (p <0.001). Clinical Relevance Statment: This study reveals that there is good agreement in the comparison of two systems designed to analyze the movement demands of each professional soccer athlete in relation to the total distance covered. Level of Evidence I; Methodological Study - Investigation of a diagnostic test.

RESUMEN Objetivo: Probar la confiabilidad entre dos instrumentos con diferentes mecanismos de análisis, ya sea por GPS (modelo GPSPORTS®) y por análisis de video (InStat For Players®), relacionando los resultados de distancia total recorrida y distancia a alta velocidad ≥ 20km / h (Distancia de carrera de muy alta intensidad, VHIR) durante los partidos oficiales de fútbol. Diseño del estudio: se trata de un estudio metodológico. En los partidos oficiales se recopilaron datos de 35 atletas de fútbol profesionales masculinos, de todas las posiciones tácticas. Edad 29,2 (± 4,8 años) y grasa corporal 9,9 (± 1,7%), excluidos los porteros (102 análisis individuales). En el análisis de datos, se utilizaron procedimientos de estadística descriptiva para caracterizar la muestra y se utilizó el coeficiente de correlación intraclase (CCI) para verificar la concordancia sobre la estabilidad y consistencia interna de las pruebas con intervalos de confianza (IC) del 95%. Resultados: El CCI en el caso de la variable distancia total recorrida fue significativa 0,914 (0,876; 0,941) e indicó una concordancia muy alta, con el coeficiente de correlación lineal indicando una fuerte correlación positiva (p <0,001). El CCI para la variable VHIR no fue significativo, aunque el coeficiente de correlación lineal indica una fuerte correlación positiva (p <0,001). Enunciado de relevancia clínica: Este estudio revela que existe un buen acuerdo en la comparación de dos sistemas diseñados para analizar las demandas de movimiento de cada deportista de fútbol profesional en relación con la distancia total recorrida. Nivel de evidencia I; Estudio Metodológico - Investigación de una prueba diagnóstica.

https://doi.org/10.1590/1517-8692202228042021_0064

10.

Effective recommendations towards healthy routines to preserve mental health during the COVID-19 pandemic
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Pilz, Luísa K.

; Couto Pereira, Natividade S.

; Francisco, Ana Paula

; Carissimi, Alicia

; Constantino, Débora B.

; Caus, Letícia B.

; Abreu, Ana Carolina O.

; Amando, Guilherme R.

; Bonatto, Fernanda S.

; Carvalho, Paula V.V.

; Cipolla-Neto, José

; Harb, Ana

; Lazzarotto, Gabriela

; Marafiga, Joseane Righes

; Minuzzi, Luciano

; Montagner, Francisco

; Nishino, Fernanda A.

; Oliveira, Melissa A.B.

; dos Santos, Bruno G.T.

; Steibel, Eduardo G.

; Tavares, Patrice S.

; Tonon, André C.

; Xavier, Nicóli B.

; Zanona, Querusche Klippel

; Amaral, Fernanda G.

; Calcagnotto, Maria Elisa

; Frey, Benicio N.

; Hidalgo, Maria Paz

; Idiart, Marco

; Russomano, Thais

Brazilian Journal of Psychiatry

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Abr 2022, Volume 44 Nº 2 Páginas 136 - 146

Resumo: En | Texto: En | PDF: En

Objective: To assess the adherence to a set of evidence-based recommendations to support mental health during the coronavirus disease 2019 (COVID-19) pandemic and its association with depressive and anxiety symptoms. Methods: A team of health workers and researchers prepared the recommendations, formatted into three volumes (1: COVID-19 prevention; 2: Healthy habits; 3: Biological clock and sleep). Participants were randomized to receive only Volume 1 (control), Volumes 1 and 2, Volumes 1 and 3, or all volumes. We used a convenience sample of Portuguese-speaking participants over age 18 years. An online survey consisting of sociodemographic and behavioral questionnaires and mental health instruments (Patient Health Questionnaire-9 [PHQ-9] and Generalized Anxiety Disorder-7 [GAD-7]) was administered. At 14 and 28 days later, participants were invited to complete follow-up surveys, which also included questions regarding adherence to the recommendations. A total of 409 participants completed the study – mostly young adult women holding university degrees. Results: The set of recommendations contained in Volumes 2 and 3 was effective in protecting mental health, as suggested by significant associations of adherence with PHQ-9 and GAD-7 scores (reflecting anxiety and depression symptoms, respectively). Conclusion: The recommendations developed in this study could be useful to prevent negative mental health effects in the context of the pandemic and beyond.

https://doi.org/10.1590/1516-4446-2021-2109

11.

Art and science: impact of semioccluded vocal tract exercises and choral singing on quality of life in subjects with congenital GH deficiency
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Andrade, Bruna M. R. de

; Valença, Eugenia H. O.

; Salvatori, Roberto

; Oliveira Neto, Luiz A.

; Souza, Anita H. O.

; Oliveira, Alaíde H. A.

; Oliveira, Mario C.P.

; Melo, Enaldo V.

; Carvalho, Susana de

; Sales, Neuza J

; Monteiro, Gisane C.

; Lima, José Marcel de

; Annunziato, Marcos Felipe Harder

; Mannis, Guilherme Daniel Breternitz

; Souza, Lucas E. de A.

; Goes, Yasmin D.

; Carvalho, Thayza S.

; Farias, Celiane de

; Santos, Michela P. dos

; Cardoso, Gabriela P. F.

; Sousa, Carla S. Pereira

; Santana, Julia Rodrigues

; Sales, Ester Almeida

; d’Avila, Jeferson Sampaio

; Aguiar-Oliveira, Manuel H.

Archives of Endocrinology and Metabolism

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Abr 2022, Volume 66 Nº 2 Páginas 198 - 205

Resumo: En | Texto: En | PDF: En

ABSTRACT Objectives: Currently, not much is known about the interactions between voice and growth hormone (GH). We have described large kindred with isolated GH deficiency (IGHD) due to a GHRH receptor mutation, resulting in severe short stature and high-pitched voice. These IGHD individuals have little interest in GH treatment, as they consider themselves “short long-lived people”, rather than patients. Interestingly, they report normal general quality of life, but they rate their Voice-Related Quality of Life (V-RQOL) as low. Here, we assessed the social and auditory-perceptual impacts of artistic-intervention voice therapy with semioccluded vocal tract exercises (SOVTE) and choral singing, on their voices. Material and methods: Seventeen GH-naïve adult IGHD individuals were enrolled in a single-arm interventional pre-post study with 13 weekly sessions of choir singing over 90 days. Outcome measures were V-RQOL scores, self-assessment of voice, and auditory-perceptual analysis (GRBAS scale, G: grade of the severity of dysphonia; R: roughness; B: breathiness; A: asthenia; and S: strain). Results: Marked improvements in total (p = 0.0001), physical (p = 0.0002), and socioemotional (p = 0.0001) V-RQOL scores and in self-assessment of voice (p = 0.004) were found. The general grades of vocal deviation (p = 0.0001), roughness (p = 0.0001), breathiness (p = 0.0001) and strain (p = 0.0001) exhibited accentuated reductions. Conclusions: Voice therapy with semioccluded vocal tract exercises and choral training improved social impact and perceptual voice assessments in IGHD subjects and markedly improved their voice-related quality of life. This is particularly important in a setting where GH replacement therapy is not widely accepted.

https://doi.org/10.20945/2359-3997000000449

12.

A New Synthetic Route and Comprehensive Topological Study of a Benzimidazole Derivative
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Duarte, Vitor S

; Naves, Raquel F

; Bortoluzzi, Adailton J

; Faria, Eduardo C. M

; Silva, Aline M. da

; Oliveira, Guilherme R

; Alonso, Christian G

; Napolitano, Hamilton B

Journal of the Brazilian Chemical Society

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Mar 2022, Volume 33 Nº 3 Páginas 291 - 302

Resumo: En | Texto: En | PDF: En

Benzimidazole and its derivatives are molecules that have diverse biological properties, therefore we synthesized and studied a new benzimidazole to understand their physical/chemical properties. The compound was obtained through a new synthetic route, using graphite oxide, in the absence of solvent, with crystalline packaging supported by C-H…N and C-H…π interactions. According to the frontier molecular orbitals, the compound is kinetically stable while the molecular electrostatic potential map confirms the site of molecular interactions involving nitrogen atoms and the π-system. This comprehensive study on synthesis, structural description and theoretical calculations can support ongoing studies in pharmaceutical science.

https://doi.org/10.21577/0103-5053.20210149

13.

In vitro germination of Abricó-de-Macaco (Couroupita guianensis aubl.)zygotic embryos in different culture media and light spectra
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Demétrio, Christian Aparecido

; Jacob, Jéssica Fernanda de Oliveira

; Ambrosano, Guilherme Bovi

; Rodrigues, Paulo Hercílio Viegas

Ciência Rural

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2022, Volume 52 Nº 1 elocation e20190906

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Couropita guianensis Aubl. é uma espécie florestal de origem amazônica, de importância no uso medicinal e ornamental. O objetivo do presente estudo foi avaliar o efeito de diferentes meios de cultivo e espectros de luz na germinação e desenvolvimento in vitro de embriões zigóticos de Couropita guianensis. Os meios de cultura avaliados foram o MS e o WPM, sem adição de reguladores de crescimento, associados a quatro espectros de luz de LED branca (CW), 70% vermelha + 30% azul (R2B), 100% vermelha (R) e 100% azul (B). Ocorreu 100% de germinação in vitro e os meios de cultivo não interferiram no desenvolvimento dos embriões. Os diferentes espectros de luz induziram a morfogênese in vitro e o tratamento R2B promoveu a produção de raízes secundárias em número significativo. Este efeito poderá auxiliar no processo de enraizamento e aclimatização de mudas dessa espécie.

ABSTRACT: Couroupita guianensis Aubl. is an Amazonian forest species with important medicinal and ornamental value. This study evaluated the effect of different culture media and light spectra on the in vitro germination and development of the zygotic embryos of C. guianensis. The culture media, MS and WPM, were evaluated without the addition of plant growth regulators and were associated with four LED light spectra: white (CW), 70% red + 30% blue (R2B), 100% red (R), and 100% blue (B). One hundred percent of the seeds successfully underwent in vitro germination, and the culture media did not interfere with embryo development. In addition to this, the different light spectra induced in vitro morphogenesis and R2B treatment significantly promoted the production of secondary roots. This effect may aid in the rooting and acclimatization of seedlings of this species.

https://doi.org/10.1590/0103-8478cr20190906 98 downloads

14.

Morphological, physiological, and agronomic traits of crossings of 'Icatu' x 'Catimor' coffee tree subjected to water deficit
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Coelho, Larissa Sousa

; Tassone, Guilherme Augusto Teixeira

; Carvalho, Gladyston Rodrigues

; Silva, Vânia Aparecida

; Viana, Mariana Thereza Rodrigues

; Pereira, Fernanda Aparecida Castro

; Nadaleti, Denis Henrique Silva

; Silveira, Helbert Rezende de Oliveira

; Botelho, Cesar Elias

Pesquisa Agropecuária Brasileira

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2022, Volume 57 elocation e02788

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste trabalho foi selecionar genótipos de Coffea arabica com boa produtividade e potencial de tolerância ao deficit hídrico, além de tentar compreender os mecanismos fisiológicos e anatômicos envolvidos na adaptabilidade desses genótipos ao estresse hídrico. Avaliaramse características fisiológicas, anatômicas e agronômicas de 19 genótipos de C. arabica sob as duas seguintes condições hídricas: irrigação regular e sem irrigação (deficit hídrico no solo). Os genótipos 'IPR 100', 2, 5 e 7 apresentaram características agronômicas, fisiológicas e anatômicas que contribuíram para melhor manutenção do estado hídrico no desenvolvimento inicial do cafeeiro. Com base nesses resultados, esses genótipos são potencialmente tolerantes ao deficit hídrico. Os genótipos 4, 10, 11, 14, 15 e 'Bourbon Amarelo IAC J10' apresentam menor adaptabilidade das estruturas anatômicas em condições de deficit hídrico no solo. Os genótipos de cafeeiro apresentam plasticidade foliar, como a espessura do parênquima paliçádico e esponjoso, e o número, posição, dimensões e mobilidade dos estômatos em condições de deficiência hídrica.

Abstract The objective of this work was to select genotypes of Coffea arabica with good yield and potential tolerance to water deficit, as well as to try to understand the physiological and anatomical mechanisms involved in the adaptability of these genotypes to water stress. The physiological, anatomical, and agronomic traits of 19 genotypes of C. arabica were evaluated under the two following water conditions: regular irrigation and no irrigation (soil water deficit). The 'IPR 100', 2, 5, and 7 genotypes showed agronomic, physiological, and anatomical traits that contributed to a better water status maintenance in the initial development of coffee plants. Based on these results, these genotypes are potentially tolerant to water deficit. The 4, 10, 11, 14, 15, and 'Bourbon Amarelo IAC J10' genotypes show a lower adaptability of the anatomical structures under soil-water deficit conditions. The coffee tree genotypes display leaf plasticity, such as the thickness of palisade and spongy parenchyma, and the number, position, dimensions, and mobility of stomata under water deficit conditions.

https://doi.org/10.1590/s1678-3921.pab2022.v57.02788

15.

Herbicide use history and perspective in South America
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Merotto Jr, Aldo

; Gazziero, Dionisio L. P.

; Oliveira, Maxwel C.

; Scursoni, Julio

; Garcia, Milton Alejandro

; Figueroa, Rodrigo

; Turra, Guilherme M.

Advances in Weed Science

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2022, Volume 40 Nº spe1 elocation e020220050

Resumo: En | Texto: En | PDF: En

Abstract Background Agriculture in South America (SA) had a great expansion in the last decades and weed control changed accordingly with region and crop practices. Objective The objective of this review is to present the history of herbicide use and discuss the main changes in weed management in SA. Methods Herbicide use quantities were obtained from official institutions and commercial organizations in Argentina, Brazil, Chile, Paraguay and Uruguay. Data were summarized per active ingredient, herbicide mode of action or crop. The evolution of the cultivated area of the main crops in each country, and the crop and weed management associated to it were considered to discuss the importance and the consequences of the main herbicides used. Results In 2019 the most used herbicides in Brazil were glyphosate, 2,4-D, atrazine, paraquat and diuron representing 62, 15, 7, 5 and 2% of the total amount used. In Argentina, the increasing selection of herbicide resistant populations (4 cases/year), resulted in utilization of older chemistries. Weed control in Uruguay is traditionally benefited from crop/pasture rotations but recently is also facing problems of continuous cropping systems. Agriculture in Chile is more diverse, but similar patterns and problems of herbicide use are present. Conclusions Intensification of agriculture, no-tillage, glyphosate resistant crops, and herbicide resistant weeds were the most important drivers of herbicide use changes in SA. Integrated weed management is unpostponable to provide sustainable increasing food production in SA.

https://doi.org/10.51694/advweedsci/2022;40:seventy-five010

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