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ABSTRACT The global reemergence of measles in 2018–2019 reinforces the relevance of high-coverage immunization to maintain the disease elimination. During an outbreak in the Sao Paulo State in 2019, several measles cases were reported in individuals who were adequately vaccinated according to the current immunization schedule recommends. This study aimed to assess measles IgG antibody seropositivity and titers in previously vaccinated adults. A cross-sectional study was conducted at CRIE-HC-FMUSP (Sao Paulo, Brazil) in 2019. It included healthy adults who had received two or more Measles-Mumps-Rubella vaccines (MMR) and excluded individuals with immunocompromising conditions. Measles IgG antibodies were measured and compared by ELISA (Euroimmun®) and chemiluminescence (LIASON®). The association of seropositivity and titers with variables of interest (age, sex, profession, previous measles, number of measles-containing vaccine doses, interval between MMR doses, and time elapsed since the last MMR dose) was analyzed. A total of 162 participants were evaluated, predominantly young (median age 30 years), women (69.8%) and healthcare professionals (61.7%). The median interval between MMR doses was 13.2 years, and the median time since the last dose was 10.4 years. The seropositivity rate was 32.7% by ELISA and 75.3% by CLIA, and a strong positive correlation was found between the tests. Multivariate analyses revealed that age and time since the last dose were independently associated with positivity. Despite being a single-center evaluation, our results suggest that measles seropositivity may be lower than expected in adequately immunized adults. Seropositivity was higher among older individuals and those with a shorter time since the last MMR vaccine dose.

https://doi.org/10.1590/s1678-9946202466004

Trabecular micro-bypass implant (iStent®) in a case of bilateral acute depigmentation of the iris
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Maestrini, Heloisa Andrade

; Maestrini, Angela Andrade

; Cenachi, Sarah Pereira de Freitas

; Massote, José Aloisio

; Lopes, Amanda Batista

; Fernandes, Thatiana Almeida Pereira

Arquivos Brasileiros de Oftalmologia

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2024, Volume 87 Nº 3 elocation e2021-0239

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT We report a case of bilateral acute depigmentation of the iris in which satisfactory intraocular pressure control was obtained after resolution of the acute disease with a trabecular implant (iStent®). A 62-year-old woman presented with bilateral simultaneous acute eye pain, photophobia, increased intraocular pressure (34 mmHg), circulating pigment in the anterior chamber, areas of depigmentation in the iris, and posterior synechiae. She had received oral amoxicillin-clavulanate and moxifloxacin for pneumonia 2 months previously. Bilateral acute depigmentation of the iris was suspected as well as a viral etiology. She received oral acetazolamide, aciclovir, and prednisone, besides topical prednisolone, betaxolol, brimonidine, dorzolamide, and atropine. The disease gradually resolved in 4 months but, after 1 year, she developed bilateral cataracts, and still needed three drugs for intraocular pressure control (16/18 mmHg). Cataract-iStent® combined surgery was performed in both eyes. One year after surgery, intraocular pressure was 11/12 mmHg, without medication. iStent® was safe and effective on this secondary glaucoma.

RESUMO Relatamos um caso de despigmentação aguda bilateral da íris, no qual obtivemos adequado controle da pressão intraocular com o implante do iStent®, após resolução da fase aguda da doença. Paciente feminina, 62 anos, atendida com quadro agudo, bilateral e simultâneo de dor ocular, fotofobia, hipertensão ocular (34 mmHg), pigmentos circulantes na câmara anterior, áreas de despigmentação iriana e sinéquias posteriores. Havia recebido amoxicilina-clavulanato e moxifloxacina orais para pneumonia 2 meses antes. Suspeitando-se de despigmentação aguda bilateral da íris ou de etiologia viral, recebeu acetazolamida, aciclovir e prednisona orais, e colírios prednisolona, betaxolol, brimonidina, dorzolamida e atropina. O quadro se resolveu gradualmente em 4 meses, porém, após 1 ano, desenvolveu catarata bilateral e ainda usava 3 colírios hipotensores (pressão intraocular 16/18 mmHg). A cirurgia combinada de catarata-iStent® foi realizada em ambos os olhos. Um ano depois, a pressão intraocular mantinha-se 11/12 mmHg, sem medicação. O iStent® foi seguro e eficaz no controle deste glaucoma secundário.

https://doi.org/10.5935/0004-2749.2021-0239

Subjective Visual Vertical and Vestibular Evoked Myogenic Potential in Meniere's disease,
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Ferreira, Maristela Mian

; Lopes, Karen de Carvalho

; Grigol, Thaís Alvares de Abreu e Silva

; Ganança, Maurício Malavasi

; Caovilla, Heloisa Helena

Brazilian Journal of Otorhinolaryngology

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Jun 2023, Volume 89 Nº 3 Páginas 485 - 493

Resumo: En | Texto: En | PDF: En

Abstract Objective To evaluate otolith function by comparing the findings of the Subjective Visual Vertical, the cervical Vestibular Evoked Myogenic Potential and the ocular Vestibular Evoked Myogenic Potential tests in patients in the inter-crisis period of unilateral definite Meniere's disease. Methods The sample consisted of an experimental group (n = 22; 10 men and 12 women, mean age 47.32 ± 12.82 years) with definite unilateral Meniere's disease and a control group (n = 14; 5 men and 9 women, with a mean age of 41.64 ± 13.45 years). They all underwent vestibular evaluation by means of Subjective Visual Vertical with the bucket method and, cervical and ocular Vestibular Evoked Myogenic Potential tests. The data were collected and subjected to statistical analysis. Results The results of the comparison of the Subjective Visual Vertical, the cervical Vestibular Evoked Myogenic Potential, the ocular Vestibular Evoked Myogenic Potential and, the association of cervical Vestibular Evoked Myogenic Potential with the ocular Vestibular Evoked Myogenic Potential showed no significant difference, indicating concordance among the tests. Conclusion The identified abnormalities and the concordance between the combined proportion of the Subjective Visual Vertical, the cervical Vestibular Evoked Myogenic Potential and the ocular Vestibular Evoked Myogenic Potential findings indicate that the association of these three tests contributes to the identification of sustained and transient otolith dysfunction in the inter-crisis of unilateral definite Meniere’s disease. Level of evidence 2.

https://doi.org/10.1016/j.bjorl.2022.11.005

Measles, mumps and rubella vaccine 12 months after hematopoietic stem cell transplantation
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Randi, Bruno Azevedo

; Fernandes, Eder Gatti

; Higashino, Hermes Ryoiti

; Lopes, Marta Heloisa

; Rocha, Vanderson Geraldo

; Costa, Silvia Figueiredo

; Sartori, Ana Marli Christovam

Revista do Instituto de Medicina Tropical de São Paulo

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2023, Volume 65 elocation e21

Resumo: En | Texto: En | PDF: En

ABSTRACT The measles, mumps and rubella (MMR) vaccine is usually recommended from 24 months after a hematopoietic stem cell transplant (HSCT). Some authors have demonstrated that the MMR vaccination can be safe from 12 months post-HSCT in non-immunosuppressed patients, as recommended by the Brazilian National Immunization Program/Ministry of Health, since 2006. The objectives of this study were to evaluate when patients received MMR vaccine after an HSCT in our care service and if there were reports of any side effects. We retrospectively reviewed the records of HSCT recipients who received at least one MMR dose in our care service, a quaternary teaching hospital in Sao Paulo city, Brazil, from 2017 to 2021. We identified 82 patients: 75.6% (90.1% in the autologous group and 45.1% in the allogeneic group) were vaccinated before 23 months post-transplantation. None reported side effects following the vaccination. Our data support that the MMR vaccination is safe from 12 to 23 months after HSCT.

https://doi.org/10.1590/s1678-9946202365021

Fatores associados a multimorbidades autorreferidas em trabalhadores da rede de saúde municipal
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Alcantara, Marcus Alessandro de

; Duarte, Ana Carolina Monteiro

; Simões, Mariana Roberta Lopes

; Barroso, Heloisa Helena

; Barbosa, Rose Elizabeth Cabral

; Fonseca, Giovanni Campos

Revista Brasileira de Saúde Ocupacional

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2023, Volume 48 elocation e2

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Objective: to estimate the prevalence of non-communicable chronic diseases (NCD) and investigate factors associated with chronic multimorbidities among workers from the municipal health network. Methods: cross-sectional study involving basic health care professionals from Diamantina, Minas Gerais, Brazil. The data were collected by an interview applying validated instruments. The studied population’s individual characteristics, habits and lifestyle, health and work conditions were identified. Logistics regression was used to build the multivariate models. Results: 203 of the 257 eligible workers participated in this research, 144 women and 59 men. The most prevalent morbidities were: overweight (27.0%), hypertension (17.2%) and depression/stress (16.2%). The presence of multimorbidities (28.6%) was significantly associated to age over 48 years old (odds ratio - OR: 4,28; confidence interval of 95% - 95%CI: 1.66;11.04), to the presence of common mental disorders (OR: 3.23; 95%CI: 1.50;6.96), whereas the adequate social support at work remained as a protection factor (OR: 0.25; 95%CI: 0.11;0.57). Conclusion: the presence of multimorbidities was associated with older age, presence of common mental disorders, and work organizational characteristics.

Resumo Objetivo: estimar a prevalência de doenças crônicas não transmissíveis (DCNT) e investigar fatores associados a multimorbidades crônicas entre trabalhadores da rede de saúde municipal. Métodos: estudo transversal com profissionais da atenção básica à saúde de Diamantina, Minas Gerais, Brasil. Os dados foram coletados por entrevista com aplicação de instrumentos validados. Houve identificação das características individuais, hábitos e estilo de vida, estado de saúde e condições de trabalho da população estudada. Utilizou-se regressão logística para construção de modelos multivariáveis. Resultados: participaram da pesquisa 203 dos 257 trabalhadores elegíveis, 144 do sexo feminino e 59 do masculino. As morbidades mais prevalentes foram: sobrepeso (27,0%), hipertensão (17,2%) e depressão/estresse (16,2%). A presença de multimorbidades (28,6%) foi significativamente associada à idade superior a 48 anos (Odds ratio - OR: 4,28; intervalo de confiança de 95% - IC95%: 1,66;11,04), à presença de transtornos mentais comuns (OR: 3,23; IC95%: 1,50;6,96), enquanto o apoio social adequado no trabalho permaneceu como fator de proteção (OR: 0,25; IC95%: 0,11;0,57). Conclusão: a presença de multimorbidades foi associada a maior idade, presença de transtornos mentais comuns e características organizacionais do trabalho.

https://doi.org/10.1590/2317-6369/35120pt2023v48e2

Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil
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Freitas, Vera Lúcia Teixeira de

; Piotto, Mariana Ramos

; Esper, Helena Rangel

; Nakanishi, Erika Yoshie Shimoda

; Fonseca, Claudia de Abreu

; Assy, João Guilherme Pontes Lima

; Berreta, Olívia Campos Pinheiro

; França, Francisco Oscar de Siqueira

; Lopes, Marta Heloísa

Revista do Instituto de Medicina Tropical de São Paulo

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2023, Volume 65 elocation e7

Resumo: En | Texto: En | PDF: En

ABSTRACT This study describes the laboratory investigation of two acute Chagas disease outbreaks that occurred in the riverside communities of Marimarituba and Cachoeira do Arua, in the Santarem municipality, Para State, located in the Northern region of Brazil, and occurred in March 2016 and August 2017, respectively. The generation of data regarding the diversity of Trypanosoma cruzi parasites circulating in the Amazon region is key for understanding the emergence and expansion of Chagas disease. This study aimed to identify T. cruzi Discrete Typing Units (DTUs) involved in two outbreaks of acute Chagas disease (ACD) directly from the patient’s biological sample. Nested and multiplex PCR targeting the 24Sα (rRNA) and mini-exon genes, respectively, were used to identify T. cruzi DTU in blood samples from patients diagnosed with ACD. The samples with positive cPCR were submitted for analysis for T. cruzi DTUs, which included 13 samples from the patients with ACD by oral transmission and two samples collected from two newborns of two women with ACD, from Marimarituba and Cachoeira do Arua. The samples were classified as T. cruzi TcIV, from Marimarituba’s outbreak, and T. cruzi TcI, from Cachoeira do Arua’s outbreak. The molecular identification of T. cruzi may increase understanding of the role of this parasite in Chagas disease’s emergence within the Amazon region, contributing to the improvement of the management of this important, but also neglected, disease.

https://doi.org/10.1590/s1678-9946202365007

Tradução e adaptação transcultural do Apraxia of Speech Rating Scale 3.5 para o português brasileiro
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Santos, Dhébora Heloísa Nascimento dos

; Lima, Ivonaldo Leidson Barbosa

; Lopes, Leonardo Wanderley

CoDAS

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2023, Volume 35 Nº 3 elocation e20220012

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Purpose To present the translation into Brazilian Portuguese and cross-cultural adaptation of the Apraxia of Speech Rating Scale (ASRS) version 3.5. Methods Validation study restricted to translation and cross-cultural adaptation. The following steps were carried out: translation and synthesis of translations; verification of applicability of the scale synthesis by judges recruited for this purpose; analysis of the relevance and feasibility of the scale calculated by the Content Validity Index (CVI), individual (CVI-I) and total (CVI-T). Eighteen speech therapists were selected. Their answers were used for the analysis of agreement (intraclass correlation coefficients - ICC) and for the calculation of the Content Validity Index (CVI). Finally, the synthesis of the translation was matched in terms of semantic, idiomatic, experiential, conceptual, syntactic, grammatical, and operational equivalence. Results The ICC ranged between 0.83 and 0.94. Six items obtained values higher than 0.9. The other items presented values between 0.8 and 0.9. The CVI-I and CVI-T had excellent values (CVI ≥ 0.78) for relevance and feasibility. Conclusion The Brazilian version of the ASRS 3.5 presents semantic, idiomatic, experiential, conceptual, and syntactic/grammatical equivalence to the original document. Thus, it is ready for the next validation steps.

RESUMO Objetivo Apresentar a tradução e adaptação transcultural do Apraxia of Speech Rating Scale (ASRS) versão 3.5 para o português brasileiro. Método Estudo de validação restrito à tradução e adaptação transcultural. Foram realizadas as seguintes etapas: tradução e síntese das traduções; verificação da aplicabilidade da síntese da escala por juízes, recrutados para tal finalidade; análise da relevância e da viabilidade da escala, calculadas pelo Índice de Validade de Conteúdo (IVC) individual (IVC-I) e total (IVC-T). Foram selecionados 18 fonoaudiólogos, cujas respostas foram utilizadas para a análise de concordância (coeficientes de correlação intraclasse - CCI) e o cálculo do Índice de Validade de Conteúdo (IVC). Por fim, a síntese da tradução foi equiparada quanto à equivalência semântica, idiomática, experiencial, conceitual, sintática, gramatical e operacional. Resultados O CCI variou entre 0,83 e 0,94. Seis itens obtiveram valores superiores a 0,9. Os demais itens apresentaram valores entre 0,8 e 0,9. O IVC-I e IVC-T apresentaram excelentes valores (IVC ≥ 0,78) para a relevância e viabilidade. Conclusão A versão brasileira do ASRS 3.5 apresenta equivalência semântica, idiomática, experiencial, conceitual e sintática/gramatical em relação ao original, dessa forma, está apta para as próximas etapas de validação.

https://doi.org/10.1590/2317-1782/20232022012pt

Safety and immunogenicity of influenza A(H3N2) component vaccine in juvenile systemic lupus erythematosus
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Aikawa, Nadia Emi

; Borba, Eduardo Ferreira

; Balbi, Verena Andrade

; Sallum, Adriana Maluf Elias

; Buscatti, Izabel Mantovani

; Campos, Lucia Maria Arruda

; Kozu, Kátia Tomie

; Garcia, Cristiana Couto

; Capão, Artur Silva Vidal

; Proença, Adriana Coracini Tonacio de

; Leon, Elaine Pires

; Duarte, Alberto José da Silva

; Lopes, Marta Heloisa

; Silva, Clovis Artur

; Bonfá, Eloisa

Advances in Rheumatology

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2023, Volume 63 elocation 55

Resumo: En | Texto: En | PDF: En

Abstract Introduction Seasonal influenza A (H3N2) virus is an important cause of morbidity and mortality in the last 50 years in population that is greater than the impact of H1N1. Data assessing immunogenicity and safety of this virus component in juvenile systemic lupus erythematosus (JSLE) is lacking in the literature. Objective To evaluate short-term immunogenicity and safety of influenza A/Singapore (H3N2) vaccine in JSLE. Methods 24 consecutive JSLE patients and 29 healthy controls (HC) were vaccinated with influenza A/Singapore/ INFIMH-16-0019/2016(H3N2)-like virus. Influenza A (H3N2) seroprotection (SP), seroconversion (SC), geometric mean titers (GMT), factor increase in GMT (FI-GMT) titers were assessed before and 4 weeks post-vaccination. Disease activity, therapies and adverse events (AE) were also evaluated. Results JSLE patients and controls were comparable in current age [14.5 (10.1–18.3) vs. 14 (9–18.4) years, p = 0.448] and female sex [21 (87.5%) vs. 19 (65.5%), p = 0.108]. Before vaccination, JSLE and HC had comparable SP rates [22 (91.7%) vs. 25 (86.2%), p = 0.678] and GMT titers [102.3 (95% CI 75.0–139.4) vs. 109.6 (95% CI 68.2–176.2), p = 0.231]. At D30, JSLE and HC had similar immune response, since no differences were observed in SP [24 (100%) vs. 28 (96.6%), p = 1.000)], SC [4 (16.7%) vs. 9 (31.0%), p = 0.338), GMT [162.3 (132.9–198.3) vs. 208.1 (150.5–287.8), p = 0.143] and factor increase in GMT [1.6 (1.2–2.1) vs. 1.9 (1.4–2.5), p = 0.574]. SLEDAI-2K scores [2 (0–17) vs. 2 (0–17), p = 0.765] and therapies remained stable throughout the study. Further analysis of possible factors influencing vaccine immune response among JSLE patients demonstrated similar GMT between patients with SLEDAI < 4 compared to SLEDAI ≥ 4 ( p = 0.713), as well as between patients with and without current use of prednisone ( p = 0.420), azathioprine ( p = 1.0), mycophenolate mofetil ( p = 0.185), and methotrexate ( p = 0.095). No serious AE were reported in both groups and most of them were asymptomatic (58.3% vs. 44.8%, p = 0.958). Local and systemic AE were alike in both groups ( p > 0.05). Conclusion This is the first study that identified adequate immune protection against H3N2-influenza strain with additional vaccine-induced increment of immune response and an adequate safety profile in JSLE. ( www.clinicaltrials.gov , NCT03540823).

https://doi.org/10.1186/s42358-023-00339-7

Impact of an educational intervention on standard precautions during the COVID-19 pandemic
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Angeloni, Natália Liberato Norberto

; Silva, Maria Heloísa do Nascimento

; Valle, Lomberto Ariel Romeu

; Sousa, Álvaro Francisco Lopes

; Valim, Marília Duarte

; Andrade, Denise de

; Fronteira, Inês

; Santos Junior, Aires Garcia dos

Revista Brasileira de Enfermagem

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2023, Volume 76 Nº 4 elocation e20220750

Resumo: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective: To evaluate the impact of an educational intervention on the knowledge of nursing professionals regarding standard precautions. Methods: This is an almost experimental study conducted with 100 nursing professionals. Data collection was performed using a sociodemographic characterization instrument and the Standard Precautions Knowledge Questionnaire. The educational intervention was based on five moments, where the approach to questions with less than 70% accuracy was intensified. Results: There was a significant difference between the scores of healthcare professionals before (16.20 ± 1.51) and after (16.90 ± 1.31) the educational intervention (W=3.336; p < 0.05). Regarding knowledge about hand hygiene after glove use, an increase in knowledge from 83% to 93% was obtained. Conclusions: A positive effect on the professionals’ knowledge was recorded, demonstrating advances regarding the strengthening of already acquired knowledge and the understanding of new knowledge.

RESUMEN Objetivo: Evaluar el impacto de una intervención educativa en el conocimiento de los profesionales de enfermería sobre las precauciones estándar. Métodos: Este es un estudio cuasiexperimental realizado con 100 profesionales de enfermería. Para la recopilación de datos, se utilizaron un instrumento de caracterización sociodemográfica y el cuestionario de Conocimiento de las Precauciones Estándar. La intervención educativa se basó en cinco momentos, en los cuales se intensificó el abordaje de cuestiones con menos del 70% de aciertos. Resultados: Hubo una diferencia significativa entre las puntuaciones de los profesionales de salud antes (16,20 ± 1,51) y después (16,90 ± 1,31) de la intervención educativa (W = 3,336; p <0,05). En relación con el conocimiento sobre la higiene de las manos después del uso de guantes, se obtuvo un aumento en el conocimiento del 83% al 93%. Conclusiones: Se registró un efecto positivo en el conocimiento de los profesionales, demostrando avances en cuanto al fortalecimiento de los conocimientos ya adquiridos y la comprensión de nuevos saberes.

RESUMO Objetivo: avaliar o impacto de uma intervenção educativa no conhecimento dos profissionais de enfermagem em relação às precauções padrão. Métodos: trata-se de um estudo quase-experimental, realizada com 100 profissionais de enfermagem. Para a coleta de dados, foram utilizados o instrumento de caracterização sociodemográfica e o questionário de Conhecimento das Precauções-Padrão. A intervenção educativa foi baseada em 05 momentos, onde se intensificou a abordagem em questões com menos de 70% de acerto. Resultados: Houve diferença significativa entre os escores dos profissionais de saúde antes (16,20 ± 1,51) e após (16,90 ± 1,31) a intervenção educativa (W=3,336; p < 0,05). Em relação ao conhecimento sobre a higiene das mãos após uso de luvas, obteve-se um aumento no conhecimento de 83% para 93%. Conclusões: registrou-se efeito positivo no conhecimento dos profissionais, demonstrando avanços no que diz respeito ao fortalecimento dos conhecimentos já adquiridos e à compreensão de novos saberes.

https://doi.org/10.1590/0034-7167-2022-0750

10.

Molecular characterization and epidemiology of Toxoplasma gondii isolates from free-range chickens in the southwest region of Goiás: new genotypes
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Domann, Natália

; Rezende, Stéfanne Rodrigues

; Fleury, Amanda Cristina Corrêa

; Barbosa, Isa Marianny Ferreira Nascimento

; Ribeiro, Isabella da Costa

; Dornelas, Júlia Batista

; Oliveira, Tainá Francisca Cardoso de

; Moura, Vanessa Oliveira Lopes de

; Storchilo, Heloísa Ribeiro

; Castro, Ana Maria de

; Garcia, João Luís

; Cardoso, Ludimila Paula Vaz

; Rezende, Hanstter Hallison Alves

Revista Brasileira de Parasitologia Veterinária

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2023, Volume 32 Nº 4 elocation e009823

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract The purpose of this study was to isolate Toxoplasma gondii from tissues of free-range chickens in the southwestern region of Goiás, to detect and molecularly characterize the genetic material of the parasite, and to determine the seroprevalence of the protozoan parasite in these animals. A seroprevalence of T. gondii antibodies of 76% (19/25) was found among the chickens, while genetic material from their tissues was detected in 56% (14/25). A total of 14 isolates was obtained in the bioassay, ten of which were considered acute, eight were considered isolates of high virulence lethal to mice, and four of low virulence, considered non-lethal but with the ability to chronify the infection. Seven of the ten isolates showed significant morphometric differences from the RH strain, in terms of nucleus-complex-apical distance, length and width. Genotyping of the acute isolates was performed by RFLP-PCR, using 11 genetic markers: SAG1, SAG2 (3’SAG2 and 5’SAG2), alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and APICO. The results were compared and classified according to the genotypes listed on the ToxoDB Platform, where different profiles were observed indicating the presence of two known genotypes (#7 and #63) and five new genotypes (NEW 3, NEW4, NEW5, NEW6, NEW 7). The results showed high seroprevalence, isolation rate, molecular detection and genotypic variations of T. gondii in free-range chickens in the southwestern region of Goiás.

Resumo O objetivo deste estudo foi isolar Toxoplasma gondii de tecidos de galinhas caipiras da região sudoeste de Goiás, detectar e caracterizar molecularmente o material genético do parasito e determinar a soroprevalência do protozoário nestes animais. A soropositividade para anticorpos anti- T. gondii, nas galinhas caipiras, foi de 76% (19/25), enquanto a detecção do material genético dos tecidos foi de 56% (14/25). Por meio do bioensaio foi obtido um total de 14 isolados, sendo 10 agudos, dos quais oito foram considerados isolados de elevada virulência, sendo letais para camundongos; e quatro de baixa virulência, com capacidade de cronificar a infecção, considerados não letais. Em sete dos dez isolados foram identificadas diferenças morfométricas significativas em relação à cepa RH, quanto à distância núcleo-complexo apical, comprimento e largura. A genotipagem dos isolados agudos por RFLP-PCR foi realizada, utilizando-se 11 marcadores genéticos SAG1, SAG2 (3´SAG2 e 5´SAG2), alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, APICO. Os resultados foram comparados e classificados de acordo com os genótipos presentes na Plataforma ToxoDB, nos quais diferentes perfis foram observados indicando a presença de dois genótipos conhecidos (#7 e #63) e cinco genótipos novos (NEW 3, NEW4, NEW5, NEW6, NEW 7). Os resultados demonstraram elevada soroprevalência, taxa de isolamento, detecção molecular e variações genotípicas de T. gondii em galinhas caipiras da região sudoeste de Goiás.

https://doi.org/10.1590/s1984-29612023069

11.

Redes sociais de trabalho entre agricultores de um município do estado do Ceará
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Martins, José Wilton Lima

; Braga, Natalia Lopes

; Baião, Darli Chahine

; Matos, Tereza Glaucia Rocha

; Carvalho, Renata Guimarães de

; Maciel, Regina Heloísa

Interações (Campo Grande)

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Dez 2022, Volume 23 Nº 4 Páginas 1235 - 1253

Resumo: Pt Es En | Texto: Pt Es En | PDF: Pt | PDF: Es | PDF: En

Resumo: Este artigo tem o objetivo de descrever e analisar redes sociais de trabalho entre agricultores de um município do estado do Ceará. Para isso, foram mapeadas as redes sociais de dois agricultores e seus contatos, totalizando oito participantes. Para a coleta de dados, foi utilizada entrevista, observação e a técnica do gerador de nomes para mapeamento das redes sociais. Para a análise dos dados, foram utilizados indicadores de redes com o auxílio dos softwares Ucinet e NetDraw e de Análise de Conteúdo. Os resultados mostraram que as duas redes de trabalho fazem uso de laços de amizade e parentesco para o seu funcionamento, mobilizando capital social para viabilizar a produção rural em uma configuração semelhante a redes socioprodutivas. Contudo, apresentam diferenças, já que uma das redes é mais desenvolvida, com laços mais diversificados e uma maior quantidade de relações de trabalho remunerado e hierarquia. Conclui-se que as redes fazem parte da organização do trabalho dos agricultores e tornam viável a produção dos bens agrícolas, seja por meio de ajuda para a realização das atividades, seja pelo acesso a informações e recursos mais variados. Resumo Ceará isso contatos participantes dados entrevista Conteúdo funcionamento socioprodutivas Contudo diferenças desenvolvida hierarquia Concluise Conclui se agrícolas atividades variados

Resumen: Este artículo tiene como objetivo describir y analizar las redes sociales de trabajo entre los agricultores de un municipio del estado de Ceará. Para ello, se mapearon las redes sociales de dos agricultores y sus contactos, totalizando ocho participantes. Para la recolección de datos, se utilizó la entrevista, la observación y la técnica generadora de nombres para el mapeo de redes sociales. Para el análisis de datos, se utilizaron indicadores de red con la ayuda de los softwares Ucinet y NetDraw y Análisis de Contenido. Los resultados mostraron que las dos redes de trabajo hacen uso de lazos de amistad y parentesco para su funcionamiento, movilizando capital social para viabilizar la producción rural en una configuración similar a las redes socioproductivas. Sin embargo, presentan diferencias, ya que una de las redes es más desarrollada, con vínculos más diversificados y con mayor número de relaciones laborales remuneradas y jerarquizadas. La conclusión es que las redes son parte de la organización del trabajo de los agricultores y viabilizan la producción de bienes agrícolas, ya sea a través de la ayuda para la realización de actividades, o mediante el acceso a información y recursos más variados. Resumen Ceará ello contactos participantes datos entrevista Contenido funcionamiento socioproductivas embargo diferencias desarrollada jerarquizadas agrícolas actividades variados

Abstract: This article aims to describe and analyze social networking among farmers in a municipality in the state of Ceará. For this, the social networking of two farmers and their contacts were mapped, totaling eight participants. For data collection, interviews, observation and the name generator technique for mapping social networking were used. For data analysis, network indicators were used with the help of Ucinet and NetDraw softwares and Content Analysis. The results showed that the two work networks make use of friendship and kinship ties for their functioning, mobilizing social capital to make rural production viable in a configuration similar to socio-productive networks. However, they present differences, since one of the networks is more developed, with more diversified ties and a greater number of paid work relationships and hierarchy. The conclusion is that the networks are part of the organization of the work of farmers and make the production of agricultural goods viable, either through help to carry out activities, or through access to more varied information and resources. Abstract Ceará this mapped participants collection interviews analysis Analysis functioning socioproductive socio productive However differences developed hierarchy activities resources

https://doi.org/10.20435/inter.v23i4.3589

12.

Brazilian Academy of Neurology practice guidelines for stroke rehabilitation: part I
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Minelli, Cesar

; Bazan, Rodrigo

; Pedatella, Marco Túlio Araújo

; Neves, Luciana de Oliveira

; Cacho, Roberta de Oliveira

; Magalhães, Sheila Cristina Sayuri Abe

; Luvizutto, Gustavo José

; Moro, Carla Heloísa Cabral

; Lange, Marcos Christiano

; Modolo, Gabriel Pinheiro

; Lopes, Bruna Correia

; Pinheiro, Elisandra Leites

; Souza, Juli Thomaz de

; Rodrigues, Guilherme Riccioppo

; Fabio, Soraia Ramos Cabette

; Prado, Gilmar Fernandes do

; Carlos, Karla

; Teixeira, Juliana Junqueira Marques

; Barreira, Clara Monteiro Antunes

; Castro, Rodrigo de Souza

; Quinan, Thalita Dayrell Leite

; Damasceno, Eduardo

; Almeida, Kelson James

; Pontes-Neto, Octávio Marques

; Dalio, Marina Teixeira Ramalho Pereira

; Camilo, Millene Rodrigues

; Tosin, Michelle Hyczy de Siqueira

; Oliveira, Bianca Campos

; Oliveira, Beatriz Guitton Renaud Baptista de

; Carvalho, João José Freitas de

; Martins, Sheila Cristina Ouriques

Arquivos de Neuro-Psiquiatria

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Jun 2022, Volume 80 Nº 6 Páginas 634 - 652

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO As Diretrizes Brasileiras para Reabilitação do AVC são fruto de um esforço conjunto do Departamento Científico de Reabilitação Neurológica da Academia Brasileira de Neurologia com o objetivo de orientar os profissionais envolvidos no processo de reabilitação para a redução da incapacidade funcional e aumento da autonomia dos indivíduos. Membros do grupo acima participaram de fóruns de discussão na web com pré-temas, seguidos de reuniões por videoconferência em que as controvérsias foram discutidas, levando a um consenso. Essas diretrizes, divididas em duas partes, focam as implicações de recentes ensaios clínicos, revisões sistemáticas e metanálises sobre reabilitação do AVC. O objetivo principal é servir de orientação a médicos, fisioterapeutas, fonoaudiólogos, terapeutas ocupacionais, enfermeiros, nutricionistas e demais profissionais envolvidos no cuidado pós-AVC. As recomendações e níveis de evidência foram adaptados de acordo com a literatura disponível atualmente. Aqui é apresentada a Parte I sobre tópicos de reabilitação na fase aguda, prevenção e tratamento de doenças e comorbidades frequentes após o AVC.

ABSTRACT The Guidelines for Stroke Rehabilitation are the result of a joint effort by the Scientific Department of Neurological Rehabilitation of the Brazilian Academy of Neurology aiming to guide professionals involved in the rehabilitation process to reduce functional disability and increase individual autonomy. Members of the group participated in web discussion forums with predefined themes, followed by videoconference meetings in which issues were discussed, leading to a consensus. These guidelines, divided into two parts, focus on the implications of recent clinical trials, systematic reviews, and meta-analyses in stroke rehabilitation literature. The main objective was to guide physicians, physiotherapists, speech therapists, occupational therapists, nurses, nutritionists, and other professionals involved in post-stroke care. Recommendations and levels of evidence were adapted according to the currently available literature. Part I discusses topics on rehabilitation in the acute phase, as well as prevention and management of frequent conditions and comorbidities after stroke.

https://doi.org/10.1590/0004-282x-anp-2021-0354

13.

COOPERATIVISMO E ASSOCIATIVISMO NO CEARÁ: FORMAÇÃO DOS EMPREENDIMENTOS E TRAJETÓRIA LABORAL DE SEUS ASSOCIADOS
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Braga, Natalia Lopes

; Maciel, Regina Heloisa

Psicologia & Sociedade

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2022, Volume 34 elocation e234435

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo A pesquisa buscou analisar a história de formação de cooperativas de trabalho, verificar a importância de atores externos nesse processo e compreender a trajetória laboral dos associados e seus ingressos nos empreendimentos. Foi realizada uma pesquisa qualitativa em três empreendimentos econômicos solidários localizados no Estado do Ceará: associação de catadores de materiais recicláveis, cooperativa de costureiras e cooperativa de produtores de castanha-de-caju, por meio de entrevistas com 24 associados. Os resultados mostraram que os grupos foram auxiliados externamente no início dos empreendimentos. A trajetória laboral dos associados apresentou, de maneira geral, aspectos semelhantes, como a precarização do trabalho, informalidade e escassez de alternativas laborais. Pode-se concluir que os empreendimentos representam uma forma de inserção social por constituírem-se em espaços de oferecimento de trabalho e renda para pessoas que, por questões históricas, geográficas, sociais e/ou culturais, encontravam-se afastadas do mercado formal.

Abstract The research aimed to analyze the history of work cooperatives, to verify the importance of external actors in this process and to understand the work trajectory of the associates and their access in the cooperatives. A qualitative research was carried out through interviews with 24 associates from three solidarity economy enterprises: an association of recyclable material collectors, a seamstress cooperative and a cashew nut producer cooperative. The results showed that the groups were helped externally at the beginning of the projects. The work trajectory of the associates presented, in general, similar aspects, such as job insecurity, informality and scarcity of work alternatives. It can be concluded that the enterprises represent a form of social insertion because they constitute spaces to offer work to people who, for historical, geographic, social or cultural reasons, were far from the formal labor market.

Resumen La investigación tuvo como objetivo analizar la historia de la formación de cooperativas de trabajo, verificar la importancia de los actores externos en este proceso y comprender la trayectoria laboral de los asociados y su ingreso en las cooperativas. Se realizó una investigación cualitativa a través de entrevistas con 24 asociados en tres empresas económicas solidarias: una asociación de recolectores de materiales reciclables, una cooperativa de costureras y una cooperativa de productores de anacardos. Los resultados mostraron que los grupos recibieron ayuda externa al comienzo de los proyectos. La trayectoria laboral de los asociados presentó aspectos similares, como la inseguridad laboral, la informalidad y la escasez de alternativas de trabajo. Se puede concluir que los grupos representan una forma de inserción social porque constituyen espacios para ofrecer trabajo a personas que, por razones históricas, geográficas, sociales y / o culturales, estaban lejos del mercado laboral formal.

https://doi.org/10.1590/1807-0310/2022v34234435

14.

Efficacy of 30% and 38% Silver Diamine Fluoride in Arresting Caries Lesions After Different Application Times: An in Vitro Study
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Rodrigues, Gabriella Fernandes

; Monteiro, Amanda Souza Nunes

; Vollú, Ana Lúcia

; Pires, Paula Maciel

; Silva, Aline Saddock de Sá

; Lopes, Ricardo Tadeu

; Oliveira, Branca Heloisa

; Neves, Aline de Almeida

; Barja-Fidalgo, Fernanda

; Fonseca-Gonçalves, Andréa

Pesquisa Brasileira em Odontopediatria e Clínica Integrada

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2022, Volume 22 elocation e210112

Resumo: En | Texto: En | PDF: En

Abstract Objective: To evaluate the efficacy of silver diamine fluoride (SDF) in arresting dentin caries lesions when applied under different concentrations and times. Material and Methods: Forty-two bovine blocks were selected and fixed in 24-well plates. Each well received a mixed bacterial inoculum added to the culture medium with 5% sucrose. The plates were incubated in microaerophilia (7 days) for caries formation, confirmed by micro-CT (M1). SDF was applied over the carious lesions for different times and concentrations (n=6): SDF 30% - immediate removal, 1 minute and 3 minutes; SDF 38%, - immediate removal, 1 minute and 3 minutes. The group without treatment was the control. Then, the samples were again scanned by micro-CT (M2) and submitted to a second cariogenic challenge for 21 days. Then, a final scan was performed (M3). Results: Mean pH at the culture medium and lesion depth were compared using Kruskal-Wallis and Wilcoxon tests. 38% SDF showed the lowest metabolic activity of the biofilm. All 38% groups and 30% 1 and 3 minutes did not show an increase in mean lesion depth comparing M3 with M1. However, only 30% 3 minutes and 38% 1 and 3 minutes showed a significant reduction of lesion depth. Conclusion: The minimum application time of 30% SDF to arrest dentin caries lesion was 1 minute, while 38% SDF arrested with application and immediate removal.

https://doi.org/10.1590/pboci.2022.019

15.

Adverse events following immunization of elderly with COVID-19 inactivated virus vaccine (CoronaVac) in Southeastern Brazil: an active surveillance study
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Miyaji, Karina Takesaki

; Itto, Lucas Yuji Umesaki

; Jacintho, Lucas Caue

; Sales, Amanda Caroline Ribeiro

; Hiratsuka, Marcel

; Leonel, Fabio Campos

; Higa-Taniguchi, Keila Tomoko

; Picone, Camila Melo

; Lara, Amanda Nazareth

; Rodrigues, Camila Cristina Martini

; Lopes, Marta Heloisa

; Sartori, Ana Marli Christovam

Revista do Instituto de Medicina Tropical de São Paulo

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2022, Volume 64 elocation e56

Resumo: En | Texto: En | PDF: En

ABSTRACT Healthcare workers, the elderly and other vulnerable populations were the first to receive COVID-19 vaccines in public health programs. There were few vaccine safety data available on the elderly. This observational study aimed to evaluate the inactivated vaccine (CoronaVac) safety in the elderly, at the beginning of the vaccination program, in Sao Paulo city, Brazil. The elderly people that received CoronaVac at the Reference Center for Special Immunobiologicals (CRIE) or at home, administered by the Interdisciplinary Home Care Team (NADI) of the Hospital das Clinicas were invited to participate in this phase 4 observational study. The vaccination schedule included two CoronaVac doses 28 days apart. The information on solicited and unsolicited adverse events following immunization were collected by phone calls on days 4 and 8 after each vaccine dose. We enrolled 158 adults aged 65 to 101 years (mean of 84.1 years); 63.9% were females and 95.6% had chronic conditions, 21.5% had moderate or severe impairment in daily living activities; 34.2% were pre-frail and 19.6% were frail. We were able to contact 95.6% and 91.6% of the vaccinated people, after the first and second doses, respectively; 31.8% and 23.4% of the contacted participants reported some adverse events (AE) following the first and second doses, respectively. Pain at the injection site, fatigue, myalgia and headaches were the most frequent solicited AE. Most AE were mild to moderate. There were eight severe adverse events, but none of them were considered related to the vaccine. The CoronaVac was safe and well tolerated by these adults of advanced age with frailty and comorbidities.

https://doi.org/10.1590/s1678-9946202264056

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