RESUMEN Objetivo. Evaluar la respuesta serológica de anticuerpos de una llama (Lama glama) a la inmunización del virus SARS-CoV-2 (linaje B.1.1) y la capacidad neutralizante del suero de llama hiperinmune frente al virus SARS-CoV-2 (linaje B.1.1) en células Vero. Materiales y métodos. Se inmunizó una llama con el virus SARS-CoV-2 inactivado (Linaje B.1.1) y se analizaron muestras de suero para evaluar el nivel de anticuerpos mediante ELISA, así como la reactividad a antígenos de SARS-CoV-2 mediante Western Blot. Además, se evaluó la neutralización viral en cultivos celulares por la Prueba de Neutralización por Reducción de Placas (PRNT, por sus siglas en inglés). Resultados. Se observó un aumento en la serorreactividad en la llama inmunizada desde la semana 4 en adelante. Los títulos de anticuerpos fueron más elevados en el séptimo refuerzo de inmunización. Los resultados de Western Blot confirmaron los hallazgos positivos del ELISA, y los anticuerpos del suero inmune reconocieron varias proteínas virales. El ensayo de neutralización (PRNT) mostró una neutralización viral visible, concordante con los resultados de ELISA y Western Blot. Conclusiones. Los hallazgos sugieren que el suero hiperinmune de llama podría constituir una fuente de anticuerpos terapéuticos contra las infecciones por el virus SARS-CoV-2 (linaje B.1.1) y que deberá ser evaluado en estudios posteriores.

ABSTRACT Objective. To evaluate the serological antibody response of a llama (Lama glama) to SARS-CoV-2 (B.1.1 lineage) immunization and the neutralizing capacity of hyperimmune llama serum against SARS-CoV-2 virus (B.1.1 lineage) in Vero cells. Materials and methods. A llama was immunized with inactivated SARS-CoV-2 (B.1.1 lineage). Serum samples were analyzed to evaluate the level of antibodies by ELISA, as well as reactivity to SARS-CoV-2 antigens by Western Blot. In addition, viral neutralization in cell cultures was assessed by the Plate Reduction Neutralization Test (PRNT). Results . Seroreactivity increased in the immunized llama from week 4 onwards. Antibody titers were the highest after the seventh immunization booster. Western blot results confirmed the positive ELISA findings, and immune serum antibodies recognized several viral proteins. The neutralization assay (PRNT) showed visible viral neutralization, which was in accordance with the ELISA and Western Blot results. Conclusions. The findings suggest that hyperimmune llama serum could constitute a source of therapeutic antibodies against SARS-CoV-2 infections (lineage B.1.1), and should be studied in further research.

RESUMO A pesquisa examina os métodos de criminalização do protesto social que se exercem por meio da mídia, como sistemas simbólicos de construção da realidade que favorecem o controle e a reprodução da ordem social. A partir do estudo de caso da marcha feminista de 8 de março de 2020 na Espanha são analisados os enquadramentos feitos pelos jornais El País, El Mundo, La Vanguardia, ABC e La Razón em suas produções informativas. O período de observação vai de 7 de março a 21 de junho de 2020, quando terminou o primeiro estado de alarme declarado pelo governo espanhol para o combate à pandemia de covid-19. Os resultados mostram que para além das linhas editoriais e ideológicas desses meios de comunicação, o protesto feminista foi demonizado principalmente pelo enquadramento identificado nos textos, por meio da construção de uma ideia geral de responsabilidade na disseminação do vírus.

ABSTRACT The research examines the methods in which social protest is criminalized through the media, as symbolic systems for the construction of reality that favor the control and reproduction of the social order. Based on the case study of the feminist demonstration on March 8, 2020, in Spain, the framing made by the newspapers El País, El Mundo, La Vanguardia, ABC and La Razón in their informative productions are analyzed. The observation period is between March 7 and June 21, 2020, when the first alarm state declared by the Spanish Government to fight the covid-19 pandemic ended. The results show that beyond the editorial and ideological lines of these media, the feminist protest was mainly demonized by the framing identified in the texts, through the construction of a general idea of responsibility in the spread of the virus.

RESUMEN A pesquisa examina os métodos de criminalização do protesto social que se exercem por meio da mídia, como sistemas simbólicos de construção da realidade que favorecem o controle e a reprodução da ordem social. A partir do estudo de caso da marcha feminista de 8 de março de 2020 na Espanha são analisados os enquadramentos feitos pelos jornais El País, El Mundo, La Vanguardia, ABC e La Razón em suas produções informativas. O período de observação vai de 7 de março a 21 de junho de 2020, quando terminou o primeiro estado de alarme declarado pelo governo espanhol para o combate à pandemia de covid-19. Os resultados mostram que para além das linhas editoriais e ideológicas desses meios de comunicação, o protesto feminista foi demonizado principalmente pelo enquadramento identificado nos textos, por meio da construção de uma ideia geral de responsabilidade na disseminação do vírus.

Resumen Introducción. La aparición de enterobacterias multirresistentes y productoras de betalactamasas de espectro extendido (BLEE) en pacientes de consulta externa con infecciones urinarias, representa un problema de salud pública en Perú. Objetivos. Caracterizar molecularmente enterobacterias multirresistentes aisladas de pacientes con diagnóstico de infección urinaria y procedentes de dos departamentos de la selva peruana. Materiales y métodos. Se hizo un estudio descriptivo, observacional y retrospectivo de 61 aislamientos de urocultivo procedentes de la selva peruana durante 2017 y 2018. Los perfiles de resistencia se identificaron utilizando el sistema automatizado MicroScan™ y para la detección de los genes bla TEM , bla CTX-M , bla SHV se empleó una reacción en cadena de la polimerasa (PCR) convencional. Resultados. Las enterobacterias positivas para BLEE más frecuentes por departamento fueron Escherichia coli en Madre de Dios (25%, 10/40) y Ucayali (76,2%, 16/21). En ambos departamentos, el gen blaCTX-M fue el más frecuente (25/61; 41), seguido por bla TEM (15/61; 24,6%) y bla SHV (10/61; 16,4%). En el perfil de sensibilidad antimicrobiana, se detectó 72,6% de resistencia contra ampicilina, 82,3 % contra cefalotina y 88,7% contra nitrofurantoína. Conclusiones. El porcentaje de cepas de enterobacterias multirresistentes productoras de BLEE en ambos departamentos fue del 57,4% y el gen bla CTX-M fue el más frecuente.

Abstract Introduction. The emergence of multiresistant enterobacteria producing extended- spectrum beta-lactamase (ESBL) in outpatients with urinary tract infections represents a public health problem in Perú. Objectives. To characterize multiresistant enterobacteria isolated from patients diagnosed with urinary tract infection in two Peruvian jungle departments using molecular techniques. Materials and methods. We conducted a descriptive, observational, and retrospective study of 61 urine culture isolates from two departments in the Peruvian jungle during 20172018. Resistance profiles were identified using the MicroScan™ automated system and a conventional polymerase chain reaction (PCR) was used for the detection of bla CTX-M , bla TEM and bla SHV genes.. Results. The most common positive ESBL enterobacteria for each department were Escherichia coli in Madre de Dios (10/40; 25%) and Ucayali (16/21; 76.2%). Gene bla CTX-M was the most prevalent in both departments (25/61; 41%), followed by bla TEM (15/61; 24.6%), and bla SHV (10/61; 16.4%). As for the antimicrobial susceptibility profile, we detected resistance levels of 72.6% for ampicillin, 82.3% for cephalothin, and 88.7% for nitrofurantoin. Conclusions. BLEE-producing multi-resistant enterobacteria strains in both departments were 57.4% and bla CTX-M was the most common gene.

ABSTRACT The aim of this study was to determine the presence of beta-lactamase- (bla) producing Enterobacteriaceae in hospital effluent samples from two level II and III hospitals in Lima, Peru. The resistance profile of the isolated bacteria was identified and characterized using the MicroScan system for 18 antimicrobials, and the presence of extended spectrum beta-lactamases (ESBL) (blaCTX-M ,bla SHV bla TEM ,bla PER) and carbapenemases (bla KPC ,bla NDM ,bla VIM ,bla IMP) resistance genes was determined by conventional PCR. Thirty-two isolates were identified (20 Enterobacteriaceae and 12 gram-negative bacteria). All the isolated bacteria showed multidrug resistance. ESBL (bla TEM) and carbapenemase (blaKPC, blaIMP) genes were found in samples from the hospitals that we evaluated. The release of these microorganisms to public areas and the lack of treatment of the hospital effluents could be an important public health problem.

RESUMEN Con el objetivo de determinar la presencia de enterobacterias productoras de betalactamasas (bla) en muestras de efluentes hospitalarios, se realizó un estudio en dos hospitales de nivel II y III de Lima, Perú. Se identificó y caracterizó el perfil de resistencia de las bacterias aisladas mediante el sistema MicroScan para 18 antimicrobianos, y mediante PCR convencional se determinó la presencia de los genes de resistencia a betalactamasas de espectro de extendido (BLEE) (bla CTX-M, bla SHV, bla TEM, bla PER) y carbapenemasas (bla KPC , bla NDM , bla VIM , bla IMP). Se identificaron 32 aislados (20 enterobacterias y 12 bacterias gramnegativas). Todas las bacterias aisladas presentaron multirresistencia. Se halló la presencia de genes BLEE (bla TEM) y carbapenemasas (bla KPC y bla IMP) en los hospitales evaluados. La liberación de estos microorganismos a la vía pública y la falta de tratamiento de los efluentes hospitalarios podría ser un importante problema de salud pública.

ABSTRACT The aim of this study was to determine the presence of beta-lactamase- (bla) producing Enterobacteriaceae in hospital effluent samples from two level II and III hospitals in Lima, Peru. The resistance profile of the isolated bacteria was identified and characterized using the MicroScan system for 18 antimicrobials, and the presence of extended spectrum beta-lactamases (ESBL) (blaCTX-M ,bla SHV bla TEM ,bla PER) and carbapenemases (bla KPC ,bla NDM ,bla VIM ,bla IMP) resistance genes was determined by conventional PCR. Thirty-two isolates were identified (20 Enterobacteriaceae and 12 gram-negative bacteria). All the isolated bacteria showed multidrug resistance. ESBL (bla TEM) and carbapenemase (blaKPC, blaIMP) genes were found in samples from the hospitals that we evaluated. The release of these microorganisms to public areas and the lack of treatment of the hospital effluents could be an important public health problem.

RESUMEN Con el objetivo de determinar la presencia de enterobacterias productoras de betalactamasas (bla) en muestras de efluentes hospitalarios, se realizó un estudio en dos hospitales de nivel II y III de Lima, Perú. Se identificó y caracterizó el perfil de resistencia de las bacterias aisladas mediante el sistema MicroScan para 18 antimicrobianos, y mediante PCR convencional se determinó la presencia de los genes de resistencia a betalactamasas de espectro de extendido (BLEE) (bla CTX-M, bla SHV, bla TEM, bla PER) y carbapenemasas (bla KPC , bla NDM , bla VIM , bla IMP). Se identificaron 32 aislados (20 enterobacterias y 12 bacterias gramnegativas). Todas las bacterias aisladas presentaron multirresistencia. Se halló la presencia de genes BLEE (bla TEM) y carbapenemasas (bla KPC y bla IMP) en los hospitales evaluados. La liberación de estos microorganismos a la vía pública y la falta de tratamiento de los efluentes hospitalarios podría ser un importante problema de salud pública.

ABSTRACT Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 μg/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 μL/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice.

RESUMEN Objetivos: Evaluar la capacidad del suero hiperinmune de llama (Lama glama) para neutralizar la letalidad del veneno de la serpiente Bothrops atrox en ratones de laboratorio. Materiales y métodos: Se calculó la dosis letal media (DL50) de un pool de venenos de serpientes de Bothrops atrox de Perú, y se midieron los títulos de anticuerpos por ensayo ELISA; así como la potencia de neutralización del suero inmune por el cálculo de la dosis efectiva media (DE50) durante el periodo de inmunización. Resultados: La DL50 del veneno fue de 3,96 µg/g, similar a otros trabajos realizados en Bothrops atrox en Perú. Los títulos de anticuerpos contra el veneno se incrementan rápidamente en la llama mostrando una rápida respuesta inmune; sin embargo, la capacidad de neutralización se incrementa más lentamente y requiere de varias dosis y refuerzos de las inmunizaciones alcanzado una DE50 de 3,30 µL/g ratón y una potencia de neutralización 3,6 mg/mL después de 15 inmunizaciones. Conclusiones: El suero hiperinmune de llama es capaz de neutralizar la letalidad del veneno de la serpiente Bothrops atrox de Perú en ratones de laboratorio.

Abstract The aim is to study the assignment of transfers to the 58 municipalities of the State of San Luis Potosi during the period 2000-2018. With a panel data of 1102 observations, a pooled regression and fixed effect model was estimated. We studied the allocation of resources from Ramo 33, of the Fondo General de Participaciones and the Fondo de Fomento Municipal. The results of this study indicated for both Ramo 33 and Fondo de Fomento Municipal, that there is no influence of policy alignment as a determinant for resource allocation. However, for the Fondo General de Participaciones there is a statistically significant evidence of increased resource allocations for those municipalities with both federal and joint political alienation. This study can be replicated in other municipalities to obtain more evidence. The proposal is original for the study of transfers to San Luis Potosí municipalities. It is concluded that political alienation is a factor that determines more transfers allocations to municipalities in the studied period.

Resumen El objetivo es estudiar la asignación de transferencias que realiza el Gobierno Federal, bajo el marco del federalismo fiscal, a los 58 municipios del estado de San Luis Potosí durante el periodo 2000-2018. Con un panel de datos de 1102 observaciones se estimó un modelo de regresión múltiple para datos agrupados y un modelo de efectos fijos. Las transferencias estudiadas fueron las del Ramo 33, del Fondo General de Participaciones y del Fondo de Fomento Municipal. Los resultados muestran que en las del Ramo 33 y del Fondo de Fomento Municipal no se observa influencia de filiación política como determinante para la asignación de recursos. Mientras que en las del Fondo General de Participaciones existe evidencia estadísticamente significativa de mayores asignaciones de recursos para aquellos municipios con filiación política tanto federal como conjunta. El estudio puede replicarse en otros municipios para obtener mayor evidencia. La propuesta es original para el estudio de transferencias hacia los municipios de San Luis Potosí. Se concluye que la filiación política es un factor que determina una mayor asignación de transferencias hacia municipios en el periodo estudiado.

ABSTRACT Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 μg/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 μL/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice.

RESUMEN Objetivos: Evaluar la capacidad del suero hiperinmune de llama (Lama glama) para neutralizar la letalidad del veneno de la serpiente Bothrops atrox en ratones de laboratorio. Materiales y métodos: Se calculó la dosis letal media (DL50) de un pool de venenos de serpientes de Bothrops atrox de Perú, y se midieron los títulos de anticuerpos por ensayo ELISA; así como la potencia de neutralización del suero inmune por el cálculo de la dosis efectiva media (DE50) durante el periodo de inmunización. Resultados: La DL50 del veneno fue de 3,96 µg/g, similar a otros trabajos realizados en Bothrops atrox en Perú. Los títulos de anticuerpos contra el veneno se incrementan rápidamente en la llama mostrando una rápida respuesta inmune; sin embargo, la capacidad de neutralización se incrementa más lentamente y requiere de varias dosis y refuerzos de las inmunizaciones alcanzado una DE50 de 3,30 µL/g ratón y una potencia de neutralización 3,6 mg/mL después de 15 inmunizaciones. Conclusiones: El suero hiperinmune de llama es capaz de neutralizar la letalidad del veneno de la serpiente Bothrops atrox de Perú en ratones de laboratorio.

Resumen Introducción. La aparición de enterobacterias multirresistentes y productoras de betalactamasas de espectro extendido en pacientes ambulatorios con infecciones urinarias representa un problema de salud pública en Perú. Objetivo. Comparar los perfiles de resistencia de Escherichia coli uropatógenas e identificar los fenotipos de cepas productoras de betalactamasas de espectro extendido en tres establecimientos privados de salud localizados en las regiones de la costa, la sierra y la selva de Perú. Materiales y métodos. Se llevó a cabo durante el 2016 un estudio descriptivo de 98 muestras de orina de pacientes con infección urinaria, 35 procedentes de Lima (costa), 38 de Juliaca (sierra) y 25 de Iquitos (selva), en el que se determinó la sensibilidad antimicrobiana utilizando ocho discos antibióticos. Asimismo, se evaluó la producción de betalactamasas de espectro extendido con discos de cefotaxima, de ceftazidima o de su combinación, con ácido clavulánico en agar Mueller-Hinton. Resultados. Se identificaron 18 perfiles de resistencia que incluían desde los sensibles a todos los antibióticos hasta los resistentes simultáneamente a siete antibióticos, con el 18,4 % de aislamientos resistentes a un antibiótico y el 54,0 % de multirresistentes. Se detectó producción de betalactamasas en el 28,6 % de las cepas procedentes de la región de Puno. También, se observó un mayor número de casos en el rango de edad de 31 a 45 años con resistencia a ceftazidima, ceftriaxona, gentamicina y trimetoprim-sulfametoxazol en el establecimiento de salud de Puno. Conclusión. Los perfiles de resistencia variaron según la localización geográfica del establecimiento de salud, observándose mayor resistencia a los antibióticos en la región de la sierra de Perú, con el 28,6 % de cepas productoras de betalactamasas de espectro extendido.

Abstract Introduction: The appearance of multidrug-resistant and beta-lactamase producing enterobacteria in outpatient care facilities represent a public health problem in Perú. Objective: To compare the resistance profiles of uropathogenic Escherichia coli and to identify extended-spectrum beta-lactamase-producing phenotypes in three private health facilities located in the Peruvian coast, Andean and jungle regions. Materials and methods: We conducted a descriptive study on 98 urine samples from Lima (coast), Juliaca (Andean region) and Iquitos (jungle region) during 2016. We determined the antimicrobial susceptibility in 35 samples from Lima, 38 from Juliaca and 25 from Iquitos using eight antibiotic disks in samples from patients diagnosed with urinary infection. We also evaluated the production of extended-spectrum beta-lactamases with cefotaxime and ceftazidime disks and a combination of both with clavulanic acid on Mueller-Hinton agar. Results: We identified 18 resistance profiles ranging from those sensitive to others simultaneously resistant to seven antibiotics: 18.4% resistant to one and 54.0% to multiple antibiotics. We detected beta-lactamase production in 28.6% of the strains from the Puno region. Likewise, we observed a greater number of cases with resistance to ceftazidime, ceftriaxone, gentamicin, and trimethoprim-sulfamethoxazole in Puno’s health facility in patients within the 31 to 45 year age range. Conclusion: Resistance profiles varied according to the geographical location of the health facilities under study. Resistance to antibiotics was higher in the Andean region with 28.6% of strains producing extended-spectrum beta-lactamases.

Resumo O trabalho aborda um dos problemas de saúde mais graves da era contemporânea: a obesidade, que aflige gravemente as crianças. Provoca inúmeros tipos de doenças e outras afecções à saúde física e mental. O objectivo do artigo foi realizar uma análise da influência da promoção da atividade física para reduzir o excesso de peso das crianças, para a qual foi realizada uma análise teórica com base na consulta de documentos especializados e atualizados. Os principais aspectos abordados foram o excesso de peso, a inatividade física e a atividade física face a problemas de obesidade, bem como o papel desempenhado pelas escolas e pelos professores na promoção da saúde face a este grave problema. O estudo realizado fornece uma base para pesquisas futuras, de tipo aplicado, em relação à intervenção que pode ser realizada a partir da classe de educação física para promover hábitos saudáveis e atividade física adequada, com o objetivo de minimizar a obesidade em crianças.

Abstract The work addresses one of the most serious health problems of the contemporary era: obesity, which seriously affects children. It causes numerous types of diseases and other affectations to physical and mental health. The objective of the article was to carry out an analysis of the influence of the promotion of physical activity to reduce the overweight of children, for which a theoretical analysis was carried out based on the consultation of specialized and updated documents. The main aspects addressed were overweight, physical inactivity and physical activity in the face of obesity problems, as well as the role played by schools and teachers in the promotion of health in the face of this serious problem. The study carried out provides a basis for future research, of an applied type, in relation to the intervention that can be carried out from the physical education class to promote healthy habits and adequate physical activity, with a view to minimizing obesity in infants.

Resumen En el trabajo, se aborda una de las problemáticas de salud más graves de la época contemporánea: la obesidad que aqueja seriamente a niños y niñas. Esta provoca numerosos tipos de enfermedades y otras afectaciones a la salud física y mental. El objetivo del artículo fue realizar un análisis de la influencia de la promoción de la actividad física para disminuir el sobrepeso en los niños, para lo cual se realizó un análisis teórico sobre la base de la consulta de documentos especializados y actualizados. Los principales aspectos abordados fueron el sobrepeso, la inactividad física y la actividad física frente a los problemas de obesidad, así como el rol que juega la escuela y el profesorado en la promoción de salud frente a esta grave problemática. El estudio realizado permite sentar bases para futuras investigaciones, de tipo aplicado, en relación con la intervención que desde la clase de educación física puede realizarse para promover hábitos sanos y una actividad física adecuada, con miras a minimizar la obesidad en los infantes.

ABSTRACT Objectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Mate rials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Results: Overex pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). Conclusion: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.

RESUMEN Objetivo: El objetivo del estudio fue analizar la expresión diferencial de miR-21, miR-29a, miR-99b y miR-155 en muestras de suero de pacientes con tuberculosis (TB) latente y TB activa respecto a contro les sanos. Materiales y métodos: Se utilizaron 28 muestras de suero (nueve con TB activa, diez con TB latente y nueve controles sanos) para el análisis de expresión génica mediante RT-qPCR con Primers y sondas TaqMan. Se calculó la expresión diferencial por el método de Livak utilizando un gen norma lizador (RNU-48). Resultados: Se halló una sobreexpresión de miR-155 en personas con tuberculosis latente, respecto a los controles sanos (0,63 vs. 0,01; valor de p=0,032). Conclusión: El miR-155 podría ser considerado un biomarcador para diferenciar TB latente de enfermedad activa. Se requieren estudios con mayores tamaños muestrales para corroborar nuestros hallazgos.

Abstract Several studies have shown cross-resistance between isoniazid and ethionamide, 2of the drugs used in the treatment of multidrug-resistant tuberculosis. The objective of this study was to determine the cross-resistance between both drugs in Mycobacterium tuberculosis isolates from a hospital with high incidence of tuberculosis in Lima, Peru. The frequency of mutations to isoniazid in the katG gene and the inhA promoter region was identified by the Genotype MTBDRplus v2.0 molecular test. The gold standard Agar Proportion method (APM) allowed todetect resistance to isoniazid and ethionamide. Of 107 isoniazid-resistant isolates (54 multidrug-resistant isolates identified by the Genotype MTBDRplus test, 45.8% (49/107) were also resistant to ethionamide by the APM. Mutations were found in the katG gene in 50.5% (54/107), in the promoter region inhA in 23.3% (25/107) and 14.0% (15/107) that share both mutations in the resistant isolates to INH. The absence of the wild type and mutation bands indicated that 12.1% (13/107) of the isolates were resistant to INH. The mutation C-15T in the inhA promoter region showed a strong association with resistance to ethionamide in 73.4% (36/49) of the isolates analyzed. The results of the present study suggest that the identification of mutations related to resistance to isoniazid, especially in the inhA promoter region, could be very useful to identify cross-resistance to ethionamide and improve the treatment of individuals suffering from this disease.

Resumen Diversos estudios han evidenciado una resistencia cruzada entre isoniacida y etionamida, 2 de los fármacos utilizados en el tratamiento de la tuberculosis multirresistente.El objetivo del presente estudio fue determinar la resistencia cruzada entre ambos fármacos en aislados de Mycobacterium tuberculosis obtenidos en un hospital de Lima (Perú), conalta proporción de pacientes con tuberculosis. Se calculó la frecuencia de mutaciones asociadas con la resistencia a la isoniacida (INH) evaluando el gen katG y la región promotorainhA mediante la prueba molecular Genotype MTBDRplus v2.0. El método gold standard conocido como agar proporciones en placa (APP) permitió la identificación de resistencia a INH yetionamida. De 107 aislamientos resistentes a INH, 54 fueron multirresistentes (identificadosmediante la prueba Genotype MTBDRplus) y 49 (es decir, el 45,8% del total) también fueronresistentes a etionamida por el método APP. En los aislamientos resistentes a INH, se encontraron mutaciones en el gen katG en el 50,5% (54/107); en la región promotora inhA en el23,3% (25/107), y un 14,0% (15/107) presentaron mutaciones en ambos. Un 12,1% (13/107)fueron resistentes a INH por ausencia de banda wild type y banda de mutación. La mutaciónC-15T en la región promotora inhA presentó una fuerte asociación con la resistencia a etionamida y alcanzó el 73,4% (36/49) de los aislamientos resistentes a dicho fármaco. Los resultadosdel presente estudio sugieren que la identificación de mutaciones relacionadas con resistenciaa INH, sobre todo en la región promotora inhA, podría ser de gran utilidad para identificarla resistencia cruzada a etionamida y mejorar el tratamiento de las personas afectadas portuberculosis.© 2019 Asociacion Argentina de Microbiolog&acuteía. Publicado por Elsevier Espana, S.L.U. Este es unart&acuteículo Open Access bajo la licencia CC BY-NC-ND (https://creativecommons.org/licenses/by-nc-nd/4.0/).

ABSTRACT Objectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Mate rials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Results: Overex pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). Conclusion: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.

RESUMEN Objetivo: El objetivo del estudio fue analizar la expresión diferencial de miR-21, miR-29a, miR-99b y miR-155 en muestras de suero de pacientes con tuberculosis (TB) latente y TB activa respecto a contro les sanos. Materiales y métodos: Se utilizaron 28 muestras de suero (nueve con TB activa, diez con TB latente y nueve controles sanos) para el análisis de expresión génica mediante RT-qPCR con Primers y sondas TaqMan. Se calculó la expresión diferencial por el método de Livak utilizando un gen norma lizador (RNU-48). Resultados: Se halló una sobreexpresión de miR-155 en personas con tuberculosis latente, respecto a los controles sanos (0,63 vs. 0,01; valor de p=0,032). Conclusión: El miR-155 podría ser considerado un biomarcador para diferenciar TB latente de enfermedad activa. Se requieren estudios con mayores tamaños muestrales para corroborar nuestros hallazgos.

Abstract Records of large crustal masses in the northwest of Borborema Province (BP) spread over more than 300 km are found as numerous granitic bodies amid a high-grade metamorphic gneissic-migmatitic terrain. The Tamboril-Santa Quitéria Complex (TSQC) is a Neoproterozoic example of these records; it is located in the north of the Ceará Central Domain (CECD) and its origin is related to a continental magmatic arc at different evolutionary stages of the arc. The Uruburetama Granite (UG), object of this study, fits into this context and constitutes one of the most representative batholiths occupying an area of 1,500 km2. Dozens of other similar plutons have been recorded in cartographic works that show similarities which suggests the grouping of these bodies in the Uruburetama Granitic Suite (UGS), previously included in the TSQC. The UG consists of a variety of plutonic rocks associated with mafic-dioritic dikes and its metamorphic products. Six petrographic facies were identified among the plutonic rocks: biotite-hornblende monzogranites and syenogranitic varieties, biotite-hornblende granodiorites, in addition to quartz syenite, leucomonzogranites, and diorites rocks. These rocks are affected by deformation, with greater intensity at the edges of the batholith, where thrust shear zones with transcurrent components were installed, generating tectonic fabrics (mylonitic foliations, stretch lineation, almond feldspar porphyroclasts, and ribbon quartz). Despite the superimposed deformational effects, magmatic relict textures are partially preserved, mainly toward the central portion of the pluton. The UG and the associated plutons are hosted by migmatized orthogneisses, paragneisses with garnet, sillimanite or kyanite, marbles, calcium-silicate gneisses, micaschists, sillimanite quartzites, and amphibolite lenses. The contact relations are diffuse with gradation for migmatites, and in rare cases, intrusive contacts with gneiss mega-xenolites are registered. The geochronological data obtained by Pb-evaporation zircon in two samples of the UG show average ages of 655 ± 2 and 656 ± 1 Ma. U-Pb zircon data for the same samples showed slightly younger age values of 559 ± 10 and 634 ± 10 Ma, respectively. The ages obtained are considered as representative of the magmatic phases of UG crystallization in the context of the evolution of the Santa Quitéria Magmatic Arc (SQMA), with the main magmatic phase of the UG in the Cryogenian. However, the age of 559 Ma would represent a younger magmatic event in the evolution of the arch. Overall, these ages correspond to the “Pre-Collisional I” or “Early-Sin-Orogenic” phase of a large collisional belt in the west of BP, and the younger ages must represent events related to the continuity of the orogen convergence of the Latest-Pre-collisional I (634 Ma) and Post-Collisional I (559 Ma) phases. Sm-Nd whole-rock isotopes data showed εNd(t) values predominantly negative (-25.6 and −0.9), and Nd-TDM model ages of 2.90 and 1.2 Ga. The results of the Lu–Hf isotopes analysis for the Bt-Hbl monzogranite sample showed negative εHf values (-26.75 to −35.48) and Hf-TDMC model ages of 3.12 to 3.65 Ga while the results for Bt-Hbl granodiorite showed εHf values of −2.07 to +1.08 and Hf-TDMC model ages of 1.46 to 1.66 Ga. These data point to the existence of two distinct and older crustal sources in the generation of these granitoids, one in the Mesoarchean, and the other in the Mesoproterozoic. The Archean ages correspond to the basement in the south of the CECD (Tróia Massif and Granjeiro Complex); on the other hand, the Mesoproterozoic ages are up for discussion, since terrains with this type of age do not occur adjacent to the BP. On the other hand, it can be interpreted as a mixture of sources, one of them probably juvenile, neoproterozoic, with contamination of the Archean crust. Thus, the UG is considered one of the most important records of the beginning of the evolution of the Santa Quitéria Magmatic Arc associated with a wide collisional belt in the “West Gondwana Orogen” in the west of Borborema Province.

Abstract The northwestern region of Borborema Province is represented by the Ceara Central and Northwest Ceara crustal blocks connected by an extensive transcurrent shear zone that represents the northern portion of the Transbrasiliano Lineament in a complex geological context, which brings together geological units of nature, origin, and ages of the Archean to the Paleozoic. In this scenario, there is a large amount of granitic bodies, with different natures, age and tectonic environment, but predominantly representing a more intense granitogenesis in the Paleozoic Neoproterozoic and early Paleozoic, with plutons emplaced in different stages of the Brazilian Orogeny. In this context, the Chaval Granite corresponds to an isolated body in the extreme northwest of Borborema Province, located near the Atlantic coast, in the northern states of Ceará and Piauí. It is an intrusive batholith housed in Siderian orthogneisses (Granja Complex) and in Neoproterozoic supracrustal rocks (Martinopole Group), but is partly covered by sedimentary rocks from the Parnaíba Paleozoic Basin (Serra Grande Group), and coastal Cenozoic deposits. Field data and petrographic studies highlight the porphyritic texture with microcline megacrystals involved in coarce matrix as a remarkable feature. The predominant petrographic types are granodiorites, with variations for monzogranites and tonalites. The predominant primary mineral constituents are pertitic microcline, oligoclase, and quartz; biotite and rarely hornblende as qualified minerals and additionally titanite, apatite, zircon, alanite, and opaque minerals. Another peculiar characteristic is the deformational features related to the installation of the Santa Rosa Transcurrent Shear Zone along its eastern flank. The effects of the transcurrent shear deformation led to the modification of the original magmatic fabrics in much of the eastern half of the batolith, generating various types of mylonites. Thus, the typically igneous textures preserved in the western half of the pluton were gradually replaced by tectonic fabrics, which initially evolved into proto-mylonites in the central portion of the body, grading to mylonites eastward, highlighting strongly stretching, comminution associated with dynamic recrystallization, highlighting the formation of microcline and plagioclase porphyroclasts in a mylonitic matrix, which are involved by mylonitic foliation. Geochemical studies reveal compositional similarities, compatible with petrographic classifications, in which they mostly present granodioritic composition, followed by monzogranites and tonalites, classified as I-type granites, peraluminous with compatible with the calcium-alkaline series. The geochemical signatures of the Chaval Granite indicate the character of the tectonic magmatic arc environment. U-Pb zircon analysis by Mass Spectrometry (LA-ICP-MS) indicates crystallization age of 633 Ma, placing it in the Neoproterozoic, late Cryogenic-Early Ediacaran period, being one of the oldest granitoids in northwestern Borborema Province, correlated to the granites of the Santa Quitéria Magmatic Arc in the Ceara Central Domain. Hf-TDM model ages (2.65 to 2.13 Ga) and εHf(t = 633 Ma) values from -9.6 to -18.1 suggest incorporation of neoarchean and paleoproterozoic crustal sources in their formation with a long crustal residence time. Similar Sm-Nd data in whole-rock indicate Nd-TDM model ages of 1.27, 1.72 and 2.04 Ga and negative εNd(t = 633 Ma) values of -2.64 to -9.13, indicating paleoproterozoic and mesoproterozoic sources, with considerable crustal residence time implying a more evolved nature.