Pesquisa | SciELO

ABSTRACT OBJECTIVE To evaluate the evolution of the dietary patterns of adolescents in the northeast region of Brazil. METHODS Secondary analysis of data from the Pesquisa de Orçamentos Familiares (POF – Household Budget Surveys), collected by the Brazilian Institute of Geography and Statistics (IBGE) in the years 2008–2009 and 2017–2018. A total of 3,095 adolescents were evaluated in 2008–2009 and 3,015 in 2017–2018. Food consumption was assessed using two dietary records in 2008–2009 and two 24-hour recalls in 2017–2018, applied on non-consecutive days. Based on these data, principal components factor analysis (PCFA) was performed, followed by orthogonal rotation of the varimax type, to derive dietary patterns, stratified by sex. The results were described as means or percentage frequencies, with their respective 95% confidence intervals. RESULTS Three main dietary patterns were identified among adolescents from the northeast region of Brazil. Among boys, in 2008–2009, the patterns were called snacks, traditional Brazilian, and coffee; and in 2017–2018, traditional Brazilian, snacks, and mixed, in this order of representativeness of the group’s eating habits. Among female adolescents, in 2008–2009, the patterns were snacks, traditional Brazilian, and coffee; and in 2017–2018, traditional Brazilian, snacks, and processed meats. CONCLUSION The dietary patterns identified in 2008–2009 and 2017–2018 were similar in both genders; however, the snacks pattern, which explained most of the data variability in 2008–2009, was replaced by the traditional Brazilian.

https://doi.org/10.11606/s1518-8787.2024058005090

Mal control y elevado riesgo cardiovascular en hipertensos de una universidad
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Morera-Galbán, Yailin

; Afonso-de-León, José Alberto

; Pérez-Cruz, Maricela

; Oliva-Correa, Elizabeth

Revista Médica Electrónica

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2024, Volume 46 elocation e5210

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Introduction: Arterial hypertension is a disease of high prevalence and fatal complications. Due to this, an adequate management is needed in the primary care, and also preventive and intervention strategies, as the HEARTS initiative in the Americas. It is also known that there is a high prevalence of hypertensive patients among the working staff of the Matanzas University of Medical Sciences. Objective: To identify the behavior of disease control and global cardiovascular risk stratification. Materials and methods: Cross-sectional descriptive study in which the first 80 hypertensive patients evaluated in the consultation of the aforementioned institution were included. The methods used were analytical-synthetic, inductive-deductive, historical-logical and statistical. Results: Of 808 workers, 276 were hypertensive, for a prevalence of 34.1%. Among the inadequate lifestyles, sedentary lifestyle predominated, with 59 patients (73.7%); 40 of the 80 patients studied had a quantitative cardiovascular risk equal to or greater than 10%, while qualitative cardiovascular risk showed that 68 (85%) had a medium or high risk. Cardiovascular diseases predominated within target organ damage, with 16.2%. Conclusions: Arterial hypertension continuous to be one of the most important risk factors for cardiovascular and cerebrovascular diseases. Its screening, control and risk assessment are a priority of the medical care at the primary level.

RESUMEN Introducción: La hipertensión arterial es una enfermedad de alta prevalencia y complicaciones mortales. Debido a ello, se necesitan un adecuado manejo en la atención primaria y estrategias preventivas y de intervención, como la iniciativa HEARTS en las Américas. Se sabe también que entre los trabajadores de la Universidad de Ciencias Médicas de Matanzas existe una elevada prevalencia de hipertensos. Objetivo: Identificar el comportamiento del control de la enfermedad y la estratificación de riesgo cardiovascular global. Materiales y métodos: Estudio descriptivo transversal en el que se incluyeron los primeros 80 pacientes hipertensos valorados en la consulta de la mencionada institución. Los métodos empleados fueron el analítico-sintético, el inductivo-deductivo, el histórico-lógico y los estadísticos. Resultados: De 808 trabajadores, 276 eran hipertensos, para una prevalencia de 34,1 %. Dentro de los inadecuados estilos de vida, predominó el sedentarismo, con 59 pacientes (73,7 %); 40 de los 80 pacientes estudiados tenían un riesgo cardiovascular cuantitativo igual o mayor del 10 %, mientras que el riesgo cardiovascular cualitativo mostró que 68 (85 %) tenían un riesgo medio o alto. Dentro del daño en órganos diana predominaron las enfermedades cardiovasculares, con un 16,2 %. Conclusiones: La hipertensión arterial sigue siendo uno de los factores de riesgo más importantes de enfermedad cardiovascular y cerebrovascular. Su pesquisa, control y estimación del riesgo constituyen una prioridad de la atención médica a nivel primario.

Experiencia didáctica durante la guía de estudiantes en proceso de sus trabajos finales de graduación
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Davis-Sánchez, Luis Alberto

; Fallas-Rodríguez, Diana Elena

Revista Educación

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Dez 2023, Volume 47 Nº 2 Páginas 615 - 639

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Objetivo: Se pretendió mostrar la experiencia docente durante la guía didáctica de estudiantes en proceso de desarrollo de sus trabajos finales de graduación en una licenciatura de la Universidad de Costa Rica. Metodología: Consistió en un diseño cualitativo descriptivo de la experiencia académica en cursos de investigación, correspondiente a Taller de graduación I y II, ambos con duración semestral. La población participante corresponde a estudiantes de dichos cursos durante los años 2020 y 2021. Se establecieron tres ejes para la recolección y análisis de los datos: a) modalidad de aprendizaje, b) abordaje teórico metodológico de los cursos y c) productos tácitos de la experiencia. Resultados: Aunque la virtualidad representó un reto para el cambio y logro de los aprendizajes, la metodología utilizada derivó en un proceso exitoso que consideró los principios generales de la pedagogía mediada por tecnología, según la Universidad de Costa Rica. El abordaje teórico metodológico permitió al estudiantado concluir sus propuestas con grado avanzado o superior en un 87.5 % y 65 % entre 2020 y 2021, respectivamente. Únicamente en el año 2020, el 62.5 % de las personas discentes tenían propuestas para someter a revisión por parte de la Comisión o aprobadas. Conclusión: Los talleres de graduación necesitan abordarse desde la metodología de investigación, en busca de claridad y secuencia en las etapas, lo cual permita verificar la viabilidad en las propuestas y priorizar el desarrollo de competencias en investigación sobre la complejidad del producto final del TFG. Aplicaciones: En cursos de guía de proyectos finales de graduación se deben establecer metas en diferentes plazos, para que el estudiantado avance de manera sostenida y consecuente. Recomendaciones: Para futuras prácticas docentes similares, se recomienda permitir a la población estudiantil egresada compartir sus experiencias con quienes apenas comienzan, y establecer cronogramas de trabajo propios para cada propuesta de investigación como herramienta de compromiso de trabajo.

Abstract As the main objective, the researchers intended to show the teaching experience during the didactic guiding of students in the process of developing their final graduation projects for a bachelor's degree from the University of Costa Rica. As for the methodology, it consisted of a qualitative descriptive design of the academic experience in research courses, corresponding to Taller de graduación I and II, both with a semester duration. The participating population consisted of students of said courses during 2020 and 2021. In addition, the authors established the following three axes for data collection and analysis: a) learning modality, b) theoretical methodological approach of the courses, and c) tacit products of experience. Regarding the results, although virtuality represented a challenge for the change and achievement of learning, the methodology resulted in a successful process that considered the general principles of technology-mediated pedagogy, according to the University of Costa Rica. Likewise, the theoretical-methodological approach allowed the student body to conclude their proposals with an advanced degree or higher in 87.5% and 65% between 2020 and 2021, respectively. Just in 2020, 62.5% of students had proposals ready to be reviewed by the commission, or approved. Hence, the authors concluded that the graduation workshops (Talleres de graduación) need to be approached from the research methodology, in search of clarity and sequence in the stages, which would allow verifying the feasibility of the proposals, as well as prioritizing the development of research competencies over the complexity of the final product of the TFG. On the other hand, concerning the applications of the research, in guidance courses for final graduation projects, goals must be established in different terms so that the student body advances sustainably and consistently. Finally, the researchers recommend for future similar teaching practices to allow the graduated student population to share their experiences with those who are just starting and to establish their own work schedules for each research proposal as a tool for a work commitment.

https://doi.org/10.15517/revedu.v47i2.52009

Frecuencia de eventos cardiovasculares en pacientes con lupus eritematoso sistémico.
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García, Lucila

; Gobbi, Carla

; Quintana, Rosana

; Alba, Paula

; Roverano, Susana

; Álvarez, Analía

; Graf, César

; Pisoni, Cecilia

; Spindler, Alberto

; Gómez, Catalina

; Figueredo, Heber Matías

; Papasidero, Silvia

; Paniego, Raúl

; de la Vega, María Celina

; Civit, Emma

; González Lucero, Luciana

; Martire, María Victoria

; Maldonado Aguila, Rodrigo

; Gordon, Sergio

; Micelli, Marina

; Nieto, Romina

; Rausch, Gretel

; Gongora, Vanina

; Damico, Agustina

; Dubinsky, Diana

; Orden, Alberto

; Zacariaz, Johana

; Romero, Julia

; Pera, Mariana

; Goñi, Mario

; Rillo, Oscar

; Báez, Roberto

; Arturi, Valeria

; González, Andrea

; Vivero, Florencia

; Bedoya, María Eugenia

; Shmid, Marcela

; Caputo, Víctor

; Larroude, María Silvia

; Domínguez, Nadia

; Gómez, Graciela

; Rodríguez, Graciela

; Marín, Josefina

; Collado, Victoria

; Jorfen, Marisa

; Bedran, Zaida

; Curti, Ana

; Gazzoni, María Victoria

; Sarano, Judith

; Finucci Curi, Pablo

; Rojas Tessel, Romina

; Arias Saavedra, Maira

; Sattler, María Emilia

; Machado Escobar, Maximiliano

; Astesana, Pablo

; París, Úrsula

; Vandale, Juan Manuel

; Santa Cruz, María Julia

; Pons-Estel, Bernardo

; García, Mercedes

Revista argentina de reumatología

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Out 2023, Volume 34 Nº 2 Páginas 43 - 50

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Introducción: el progreso en los tratamientos para el lupus eritematoso sistémico (LES) resultó en una disminución de la mortalidad; sin embargo, la enfermedad cardiovascular y las complicaciones infecciosas aún son las principales causas de muerte. La evidencia apoya la participación del sistema inmunológico en la generación de la placa aterosclerótica, así como su conexión con las enfermedades autoinmunes. Objetivos: describir la frecuencia de eventos cardiovasculares (ECV) en el Registro de Lupus Eritematoso Sistémico de la Sociedad Argentina de Reumatología (RELESSAR) transversal, así como sus principales factores de riesgo asociados. Materiales y métodos: estudio descriptivo y transversal para el cual se tomaron los pacientes ingresados en el registro RELESSAR transversal. Se describieron las variables sociodemográficas y clínicas, las comorbilidades, score de actividad y daño. ECV se definió como la presencia de al menos una de las siguientes patologías: enfermedad arterial periférica, cardiopatía isquémica o accidente cerebrovascular. El evento clasificado para el análisis fue aquel posterior al diagnóstico del LES. Se conformaron dos grupos macheados por edad y sexo 1:2. Resultados: 1515 pacientes mayores de 18 años participaron del registro. Se describieron 80 pacientes con ECV (5,3%). En este análisis se incluyeron 240 pacientes conformando dos grupos. La edad media fue de 47,8 (14,4) y 47,6 (14,2) en el grupo con y sin ECV respectivamente. Los pacientes con ECV tuvieron mayor duración del LES en meses, mayor índice de Charlson, mayor SLICC (Systemic Lupus International Collaborating Clinics/American College of Rheumatology), mayor frecuencia de manifestaciones neurológicas, síndrome antifosfolípido, hospitalizaciones y uso de ciclofosfamida. Las únicas variables asociadas en el análisis multivariado fueron el índice de Charlson (p=0,004) y el SLICC (p<0,001). Conclusiones: los ECV influyen significativamente en nuestros pacientes, y se asocian a mayor posibilidad de daño irreversible y comorbilidades.

Abstract Introduction: progress in treatments for systemic lupus erythematosus (SLE) has resulted in a decrease in mortality; however, cardiovascular and infectious diseases remain the leading causes of death. Evidence supports the involvement of the immune system in the generation of atherosclerotic plaque, as well as its connection to autoimmune diseases. Objectives: to describe the frequency of cardiovascular disease (CVD) in the cross-sectional RELESSAR registry, as well as its associated variables. Materials and methods: a descriptive and cross-sectional study was performed using patients admitted to the cross-sectional RELESSAR registry. Sociodemographic variables, clinical variables, comorbidities, activity and damage scores were described. CVD was defined as at least one of the following: peripheral arterial disease, ischemic heart disease, or cerebrovascular accident. All patients with at least one CVD were included in our analysis (heart attack, central nervous system vascular disease, and peripheral arteries atherosclerotic disease). The event classified for the analysis was that after the diagnosis of SLE. SLE diagnosis was previous to CVD. Two groups matched by age and sex, 1:2 were formed. Results: a total of 1515 patients older than 18 years participated in the registry. Eighty patients with CVD (5.3%) were described in the registry. Two-hundred and forty patients were included, according to two groups. The mean age was 47.8 (SD 14.4) and 47.6 (SD 14.2) in patients with and without CVD, respectively. Patients with CVD had a longer duration of SLE in months, a higher Charlson index, a higher SLICC, increased frequency of neurological manifestations, antiphospholipid syndrome, hospitalizations, and use of cyclophosphamide. The associated variables in the multivariate were the Charlson Index (p=0.004) and the SLICC (p<0.001). Conclusions: CVDs have a significant influence on our patients, being associated with a greater possibility of damage and comorbidities.

https://doi.org/10.47196/rar.v34i2.747

Algunas reflexiones sobre la actualización de los médicos
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Ruiz-Argüelles, Guillermo J.

; Rocha-Arrieta, Luisa L.

; Arrieta, Oscar

; Celis, Miguel A.

; Domínguez-Cherit, Judith

; Islas-Andrade, Sergio

; Lifshitz, Alberto

; Mansilla-Olivares, Armando

; Martínez, Iris

; Mimenza, Alberto José

; Moreno, Mucio

; Reyes-Sánchez, Alejandro A.

; Soda-Merhy, Antonio

; Sotelo, Julio

; Toussaint, Sonia

; Vilar-Compte, Diana

; Verástegui, Emma

Gaceta médica de México

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Ago 2023, Volume 159 Nº 4 Páginas 284 - 285

Resumo: Es | Texto: Es | PDF: Es

https://doi.org/10.24875/gmm.23000137

Variables orientadas a la prevención de la violencia contra las mujeres en bachillerato
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Treviño Villarreal, Diana Carolina

; González Medina, Mario Alberto

RIDE. Revista Iberoamericana para la Investigación y el Desarrollo Educativo

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Jun 2023, Volume 13 Nº 26 elocation e051

Resumo: Es En Pt | Texto: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen La violencia contra las mujeres es un tema que ha resonado a través de los años y se ha manifestado al interior del espacio escolar. Asimismo, se ha relacionado con variables como el sentido de pertenencia, la autoeficacia, el miedo al fracaso y el apoyo emocional parental. Por tanto, el objetivo de este estudio fue determinar la relación entre dichas variables. La muestra estuvo compuesta por 3,826 mujeres y se utilizó el cuestionario de PISA 2018, junto con índices de escala (variables). Para comprobar el modelo teórico, se aplicó el análisis de senderos, llegándose a encontrar relaciones directas del sentido de pertenencia, miedo al fracaso y apoyo emocional parental con la violencia escolar, así como una relación indirecta de la autoeficacia con la violencia escolar. En conclusión, es importante que las escuelas tomen en cuenta las variables analizadas para prevenir la violencia escolar contra las mujeres en el bachillerato.

Abstract Violence against women is a topic that has resonated through the years and this has been manifested within the school space, likewise, it has been related to variables such as the sense of belonging, self-efficacy, fear of failure and the parental emotional support, therefore, the objective was to determine the relationship between these variables. It was a sample of 3,826 women; the instrument used was the PISA 2018 questionnaire and scale indices (variables) were used. To verify the theoretical model, the path analysis was applied, finding direct relationships between the sense of belonging, fear of failure and parental emotional support with school violence and an indirect relationship between self-efficacy and school violence. In conclusion, it is necessary that schools take into account the variables analyzed since they will be preventing school violence in baccalaureate women.

Resumo A violência contra a mulher é um tema que tem repercutido ao longo dos anos e tem se manifestado no espaço escolar. Da mesma forma, tem sido relacionado a variáveis como o sentimento de pertencimento, autoeficácia, medo do fracasso e suporte emocional dos pais. Portanto, o objetivo deste estudo foi determinar a relação entre essas variáveis. A amostra foi composta por 3.826 mulheres e foi utilizado o questionário PISA 2018, juntamente com os índices da escala (variáveis). Para verificar o modelo teórico, aplicou-se a path analysis, encontrando relações diretas entre o sentimento de pertença, o medo do fracasso e o suporte emocional parental com a violência escolar, bem como uma relação indireta entre a autoeficácia e a violência escolar. Em conclusão, é importante que as escolas levem em consideração as variáveis analisadas para prevenir a violência escolar contra a mulher no ensino médio.

https://doi.org/10.23913/ride.v13i26.1493

Investigación clínica e industria farmacéutica
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Mimenza-Alvarado, Alberto J.

; Arrieta, Oscar

; Celis, Miguel A.

; Domínguez-Cherit, Judith

; Islas-Andrade, Sergio

; Lifshitz, Alberto

; Mansilla, Armando

; Martínez, Iris

; Moreno, Mucio

; Reyes-Sánchez, Alejandro A.

; Rocha-Arrieta, Luisa L.

; Argüelles, Guillermo J. Ruiz

; Sotelo, Julio

; Verástegui, Emma

; Vilar-Compte, Diana

; Toussaint, Sonia

Gaceta médica de México

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Abr 2023, Volume 159 Nº 2 Páginas 91 - 92

Resumo: Es | Texto: Es | PDF: Es

https://doi.org/10.24875/gmm.22000279

Transmisión espacial de precios desde el mercado internacional al mercado mexicano de leche
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Martínez-Marcos, Diana

; Barrios-Puente, Gerónimo

; Pérez-Soto, Francisco

; Ruiz-Hernández, Jaime Antonio

; Lara-Reimers, Eduardo Alberto

Ecosistemas y recursos agropecuarios

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Abr 2023, Volume 10 Nº 1 elocation e3022

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen. México importa aproximadamente el 25% de la leche consumida en el país, dicha cantidad es comparable al 30% de la producción nacional. Debido a esto, el precio al productor mexicano podría estar influenciado por el precio internacional de la leche. Considerando lo anterior, el objetivo de esta investigación fue analizar la transmisión de precios entre el mercado internacional y el mercado mexicano de leche en los ámbitos nacional, regional (Jalisco) y municipal (Chicontepec, Veracruz). Para ello, se determinó la estacionariedad de las series con la prueba aumentada de Dickey-Fuller, se examinó la cointegración con la prueba de Johansen, se estimó un modelo de corrección de errores y un modelo asimétrico de corrección de errores. Los resultados indican que los precios de leche al productor en México están cointegrados con los precios de Oceanía, la Unión Europea y Estados Unidos, siendo los últimos los que tienen mayores efectos en el precio al productor en México y que se transmiten al mercado mexicano de forma simétrica. Además, se encontró que la velocidad de ajuste del precio al productor ante desviaciones del equilibrio es mayor para el ámbito regional seguido del nacional y municipal. Lo que implica que las variaciones en el precio internacional afectan de forma diferente a los productores de leche de México.

Abstract. Mexico imports approximately 25% of the milk consumed in the country, this quantity is comparable to 30% of domestic production. Because of this, the Mexican producer price might be influenced by the international milk price. Therefore, the purpose of this research was to analyze price transmission between the international market and the Mexican milk market in the national, regional (Jalisco), and municipal (Chicontepec, Veracruz) scope. For this, stationarity of the series was determined using the Augmented Dickey-Fuller test, cointegration was examined using the Johansen test, and an error correction model and an asymmetric error correction model were estimated. The results indicate that milk producer prices in Mexico are cointegrated with the Oceania, the European Union, and the United States prices, being the latter the ones that have the greatest effects on the producer price in Mexico and are transmitted in a symmetric way to the Mexican market. Besides, it was found that the speed of adjustment of the producer price in the event of deviations from equilibrium is superior for the regional scope, followed by the national and the municipal scope. Meaning that the variations of the international price affect the Mexican milk producers in a different way.

https://doi.org/10.19136/era.a10n1.3022

Cambios en el recuento de cuatro grupos bacterianos durante la maduración del Queso de Prensa (Costeño) de Cuajinicuilapa, México
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Mendoza-Cuevas, José Alberto

; Santos-Moreno, Armando

; Rosas-Barbosa, Beatriz Teresa

; Ybarra-Moncada, Ma. Carmen

; Flores-Girón, Emmanuel

; Guerra-Ramírez, Diana

Revista mexicana de ciencias pecuarias

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Mar 2023, Volume 14 Nº 1 Páginas 94 - 109

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Prensa Cheese, also called Costeño, is made in an artisanal way from raw cow’s milk in the Costa Chica region of the state of Guerrero. In order to know the characteristics of Mexican artisanal cheeses, the objective of this research was to analyze the changes in the count of aerobic mesophilic bacteria (AMB), total coliform (TCs) microorganisms, lactic acid bacteria (LAB) and coagulase-positive staphylococci (CPS), during the ripening (5, 30, 60 and 90 d) of Prensa cheeses, made by four different cheese factories (A, B, C and D) of Cuajinicuilapa, Guerrero, Mexico. A portion (25 g) of each cheese sample was homogenized with peptone diluent (225 mL) and dilutions from 10-1 to 10-6 were prepared with which 3MTM PetrifilmTM plates were sown. After incubating under different conditions, depending on the type of microorganism, AMB, TCs, LAB and CPS counts were made. The results showed that as the ripening time of the Prensa Cheese progressed, the microbial load decreased: AMB from 4 to 2, TCs from 6 to 3, LAB from 6 to 2 and CPS from 5 to 2 log10 CFU g-1. The changes in the counts of the bacterial groups studied can be attributed to the physicochemical and microbiological transitions typical of cheese maturation and to the characteristics of the microbiota present in each of the cheese factories. The results of this research provide elements for the microbial characterization of Mexican artisanal cheeses.

Resumen El Queso de Prensa, también llamado Costeño es elaborado artesanalmente a partir de leche cruda de vaca en la región de la Costa Chica del estado de Guerrero. Con el fin de conocer las características de los quesos artesanales mexicanos, el objetivo de esta investigación fue analizar los cambios en el recuento de bacterias mesófilas aerobias (BMA), microorganismos coliformes totales (CT), bacterias ácido lácticas (BAL) y estafilococos coagulasa positivos (ECP), durante la maduración (5, 30, 60 y 90 días) de quesos de prensa, elaborados por cuatro diferentes queserías (A, B, C y D) de Cuajinicuilapa, Guerrero, México. Una porción (25 g) de cada muestra de queso se homogeneizó con diluyente de peptona (225 ml) y se prepararon diluciones desde 10 -1 a 10 -6 con las que se sembraron placas 3M TM PetrifilmTM. Después de incubar a diferentes condiciones, según el tipo de microorganismo, se hicieron recuentos de BMA, CT, BAL y ECP. Los resultados mostraron que conforme avanzó el tiempo de maduración del Queso de Prensa, la carga microbiana disminuyó: BMA de 4 a 2, CT de 6 a 3, BAL de 6 a 2 y ECP de 5 a 2 log10 UFC g-1. Los cambios en los recuentos de los grupos bacterianos estudiados, pueden ser atribuidos a las transiciones fisicoquímicas y microbiológicas propias de la maduración del queso y a las características de la microbiota presente en cada una de las queserías. Los resultados de esta investigación aportan elementos para la caracterización microbiana de los quesos artesanales mexicanos.

https://doi.org/10.22319/rmcp.v14i1.6121

10.

Cannabinoides y su uso terapéutico
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Islas-Andrade, Sergio

; Rocha-Arrieta, Luisa L.

; Arrieta, Oscar

; Celis, Miguel A.

; Domínguez-Cherit, Judith

; Lifshitz, Alberto

; Mansilla-Olivares, Armando

; Martínez, Iris

; Mimenza, Alberto José

; Moreno, Mucio

; Reyes-Sánchez, Alejandro A.

; Ruiz-Argüelles, Guillermo J.

; Soda-Merhy, Antonio

; Sotelo, Julio

; Toussaint, Sonia

; Vilar-Compte, Diana

; Verástegui, Emma

Gaceta médica de México

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Fev 2023, Volume 159 Nº 1 Páginas 1 - 2

Resumo: Es | Texto: Es | PDF: Es

https://doi.org/10.24875/gmm.22000184

11.

Gene silencing induced by the vector EuMY-YP∆CHS4 during the Fusarium sp. - Nicotiana benthamiana interaction
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Hau-Yama, Nidia Esther

; Luna-Rivero, Marianne Soledad

; Canto-Canché, Blondy Beatriz

; Minero-García, Yereni

; Hernández-Zepeda, Cecilia

; Sánchez-Rangel, Diana

; Moreno-Valenzuela, Oscar Alberto

Revista mexicana de fitopatología

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2023, Volume 41 Nº 3 Páginas 434 - 459

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen. En la literatura, se han propuesto métodos basados en el ARN de interferencia para el control de hongos patógenos en plantas, como el silenciamiento mediado por virus (VIGS). En este trabajo se evaluó la funcionalidad y la eficiencia del vector VIGS EuMV-YP∆CHS4, el cual contiene un fragmento de la secuencia del gen de la quitina sintasa 4 (CHS4) de Fusarium sp. y fue construido a partir del vector EuMV-YP:∆AV1. Se evaluó el vector VIGS en dos condiciones en plantas de Nicotiana benthamiana: plantas con inoculación previa con Fusarium sp. y en plantas con inoculación del hongo posterior a la inoculación del vector VIGS. Los resultados mostraron que la aplicación de EuMV-YP∆CHS4 en ambos tratamientos provocó un retardo en la caída de hojas durante los primeros días de la infección y una disminución significativa del daño en el tallo de las plantas infectadas, comparadas con el control infectado con el hongo. Además, se observó una reducción de hasta 30% en la expresión relativa del gen CHS4. Estos resultados sugieren que la aplicación de los vectores VIGS puede ser una estrategia eficiente para proteger a las plantas contra Fusarium sp.

Abstract. In the literature, RNA-based interference methods have been proposed for the control of pathogenic fungi in plants, such as virus-mediated silencing (VIGS). The objective of this work was to evaluate the functionality and efficiency of the VIGS EuMV-YP∆CHS4 vector, which contains a fragment of the Fusarium sp. chitin synthase 4 (CHS4) gene sequence, and was constructed from the EuMV-YP vector:∆AV1. The VIGS vector was evaluated under two conditions in Nicotiana benthamiana plants previously inoculated with Fusarium sp. and in plants with inoculation after the inoculation of the VIGS vector. The results showed that in both treatments with the EuMV-YP∆CHS4, a delay in leaf fall during the first days of infection and a decrease in the stem damage of almost one centimeter, compared to the control infected with the fungus. In addition, a reduction of up to 30% in the relative expression of the CHS4 gene was observed. These results suggest that the application of VIGS vectors may be efficient as a strategy to protect plants against Fusarium sp.

https://doi.org/10.18781/r.mex.fit.2305-4

12.

Validación de dominios y competencias por grupos focales y juicio de expertos
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Leis Márquez, María Teresa

; Hernández Rodríguez, César Alberto

; Sierra Lozada, Nancy

; Fregoso Ito, Diana Anahí

; Mancera Reséndiz, Miguel Ángel

Ginecología y obstetricia de México

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2023, Volume 91 Nº 9 Páginas s1 - s50

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen El Consejo Mexicano de Ginecología y Obstetricia certifica a los especialistas para ejercer su especialidad y brindar una atención de alta calidad a las pacientes. En la actualidad, el Consejo está rediseñando el examen de Certificación en Ginecología y Obstetricia orientado a la evaluación de competencias profesionales a partir de las actividades profesionales confiables que permitan identificar los dominios de la competencia médica en un especialista. La competencia es una variedad de habilidades ejercidas a través de múltiples dominios o aspectos del desempeño profesional; sus descriptores requieren que contengan habilidades relevantes, contexto y la etapa o nivel de evaluación. Por su parte, los dominios son un conjunto de competencias clave. Este informe incluye los resultados de la primera validación en México de dominios y competencias para la evaluación de los ginecoobstetras a partir de una metodología cualitativa que comprende una revisión de la bibliografía, grupos focales, análisis de trabajos colegiados y validación por juicio de expertos. Los resultados se presentan divididos por los ocho dominios en los que se trabajó, se mencionan los comentarios más relevantes a discutir por los cuatro grupos focales. Éstos y la validación por expertos permitió reunir comentarios valiosos, coherentes y funcionales para el sistema de evaluación que quiere llevar a cabo el Consejo. Este ejercicio permitirá el posterior desarrollo de la tabla de especificaciones, reactivos o nuevos instrumentos de evaluación coherentes con un sistema de dominios, competencias y actividades profesionales confiables.

Abstract The Mexican Council of Gynecology and Obstetrics certifies specialists to practice their specialty and provide high-quality healthcare to patients. Currently, the Council is redesigning the Gynecology and Obstetrics Certification exam, oriented to the evaluation of professional competencies based on reliable professional activities that allow the identification of domains of a specialist’s medical competencies. Competency can be defined as a variety of skills across multiple domains or aspects of professional performance. Its descriptors require to contain relevant skills, context, and the stage or level of assessment. Domains can be described as a set of competencies that are considered essential. This report includes the results of the first validation done in Mexico. The validation of domains and competencies for the evaluation of physicians in the area of Obstetrics and Gynecology is based on a qualitative methodology that includes a literature review, focus groups, analysis of collegiate works, and validation through expert judgment. The obtained results are divided into eight domains that mention the most relevant observations that were discussed by the four focus groups. The focus groups and the validation through expert judgment made it possible to gather valuable, coherent, and functional feedback for the evaluation system that the Council wants to carry out. This method will allow the subsequent development of the table of specifications, items, or new evaluation instruments congruent with a system of domains, competencies, and reliable professional activities.

https://doi.org/10.24245/gom.v91i9.9153

13.

Assessment of the regenerative potential of macro-porous chitosan-calcium simvastatin scaffolds on bone cells
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GALLINARI, Marjorie de Oliveira

; BORDINI, Ester Alves Ferreira

; STUANI, Vitor de Toledo

; CASSIANO, Fernanda Balestrero

; MELO, Camila Correa da Silva Braga de

; ALMEIDA, Juliano Milanezi de

; CINTRA, Luciano Tavares Ângelo

; DE SOUZA COSTA, Carlos Alberto

; SOARES, Diana Gabriela

Brazilian Oral Research

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2023, Volume 37 elocation e018

Resumo: En | Texto: En | PDF: En

Abstract This study evaluated the bioactive potential of a macro-porous chitosan scaffold incorporated with calcium hydroxide (CH-Ca) and functionalized with bioactive doses of simvastatin (SV) for bone tissue regeneration. Initially, the bioactive dose of SV in osteoblastic cells (SAOS-2) was determined. For the direct contact experiment, SAOS-2 cells were plated on scaffolds to assess cell viability and osteogenic differentiation. The second assay was performed at a distance using extracts from scaffolds incubated in culture medium to assess the effect of conditioned medium on viability and osteogenic differentiation. The initial screening showed that 1 μM SV presented the best biostimulating effects, and this dose was selected for incorporation into the CH-Ca and pure chitosan (CH) scaffolds. The cells remained viable throughout the direct contact experiment, with the greatest cell density in the CH-Ca and CH-Ca-SV scaffolds because of their higher porosity. The CH-Ca-SV scaffold showed the most intense bio-stimulating effect in assays in the presence and absence of osteogenic medium, leading to an increased deposition of mineralized matrix. There was an increase in the viability of cells exposed to the extracts for CH-Ca, CH-SV, and CH-Ca-SV during the one-day period. There was an increase in ALP activity in the CH-Ca and CH-Ca-SV; however, the CH-Ca-SV scaffold resulted in an intense increase in the deposition of mineralized nodules, approximately 56.4% at 7 days and 117% at 14 days, compared with CH (control). In conclusion, functionalization of the CH-Ca scaffold with SV promoted an increase in bioactivity, presenting a promising option for bone tissue regeneration.

https://doi.org/10.1590/1807-3107bor-2023.vol37.0018

14.

Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions
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MENDES SOARES, Igor Paulino

; ANSELMI, Caroline

; PIRES, Maria Luiza Barucci Araujo

; RIBEIRO, Rafael Antonio de Oliveira

; LEITE, Maria Luísa

; SOARES, Diana Gabriela

; DE SOUZA COSTA, Carlos Alberto

; HEBLING, Josimeri

Journal of Applied Oral Science

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2023, Volume 31 elocation e20230032

Resumo: En | Texto: En | PDF: En

Abstract Simulating a bacterial-induced pulpitis environment in vitro may contribute to exploring mechanisms and bioactive molecules to counteract these adverse effects. Objective To investigate the chronic exposure of human dental pulp cells (HDPCs) to lipopolysaccharides (LPS) aiming to establish a cell culture protocol to simulate the impaired odontogenic potential under pulpitis conditions. Methodology HDPCs were isolated from four healthy molars of different donors and seeded in culture plates in a growth medium. After 24 h, the medium was changed to an odontogenic differentiation medium (DM) supplemented or not with E. coli LPS (0 - control, 0.1, 1, or 10 µg/mL) (n=8). The medium was renewed every two days for up to seven days, then replaced with LPS-free DM for up to 21 days. The activation of NF-κB and F-actin expression were assessed (immunofluorescence) after one and seven days. On day 7, cells were evaluated for both the gene expression (RT-qPCR) of odontogenic markers (COL1A1, ALPL, DSPP, and DMP1) and cytokines (TNF, IL1B, IL8, and IL6) and the production of reactive nitrogen (Griess) and oxygen species (Carboxy-H2DCFDA). Cell viability (alamarBlue) was evaluated weekly, and mineralization was assessed (Alizarin Red) at 14 and 21 days. Data were analyzed with ANOVA and post-hoc tests (α=5%). Results After one and seven days of exposure to LPS, NF-κB was activated in a dose-dependent fashion. LPS at 1 and 10 µg/mL concentrations down-regulated the gene expression of odontogenic markers and up-regulated cytokines. LPS at 10 µg/mL increased both the production of reactive nitrogen and oxygen species. LPS decreased cell viability seven days after the end of exposure. LPS at 1 and 10 µg/mL decreased hDPCs mineralization in a dose-dependent fashion. Conclusion The exposure to 10 µg/mL LPS for seven days creates an inflammatory environment that is able to impair by more than half the odontogenic potential of HDPCs in vitro, simulating a pulpitis-like condition.

https://doi.org/10.1590/1678-7757-2023-0032

15.

Multidisciplinary Scientific Cruises for Environmental Characterization in the Santos Basin – Methods and Sampling Design
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Moreira, Daniel L.

; Dalto, Adriana G.

; Figueiredo JR, Alberto G.

; Valerio, Aline M.

; Detoni, Amalia M. S.

; Bonecker, Ana C. T.

; Signori, Camila N.

; Namiki, Cláudia

; Sasaki, Dalton K.

; Pupo, Daniel V.

; Silva, Danilo A.

; Kutner, Deborah S.

; Duque-Castaño, Diana C.

; Marcon, Eduardo H.

; Gallotta, Fabiana D. C.

; Paula, Fabiana S.

; Gallucci, Fabiane

; Roque, Gabriela C. F.

; Campos, Giulia S.

; Fonseca, Gustavo

; Mattos, Gustavo

; Lavrado, Helena P.

; Silveira, Ilson C. A. da

; Costa, Jessica O.

; Santos Filho, João R. dos

; Carneiro, Juliane C.

; Moreira, Julio C.F.

; Rozo, Laura

; Araujo, Leandro F.M.

; Lazzari, Letícia

; Silva, Letícia O. da

; Michelazzo, Luan S.

; Fernandes, Luciano F.

; Dottori, Marcelo

; Araújo Jr., Marcus A. G. de

; Chuqui, Mateus G.

; Ceccopieri, Milena

; Borges-Silva, Milton

; Kampel, Milton

; Bergo, Natascha M.

; Silva, Paulo V. M.

; Tura, Pedro M.

; Moura, Rafael B. de

; Romano, Renato G.

; Martins, Renato P.

; Carreira, Renato S.

; Toledo, Rodrigo G.A.

; Bonecker, Sérgio L.C.

; Disaró, Sibelle T.

; Rodrigues, Silvana V.

; Corbisier, Thais N.

; Vicente, Thaisa M.

; Paiva, Vitor G. de

; Pellizari, Vivian H.

; Belo, Wellington C.

; Brandini, Frederico P.

; Sousa, Silvia H.M

Ocean and Coastal Research

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2023, Volume 71 Supl. 3 elocation e23022

Resumo: En | Texto: En | PDF: En

Abstract The Santos Basin (SB) is the main petroliferous basin in the Brazilian continental margin and one of the most studied marine areas in Brazil. However, historical data suggest that new efforts should be carried out to acquire quantitative biological data, especially in the deep sea, to establish the baseline of essential ocean variables in different ecosystems for future monitoring programs. The Brazilian energy company Petrobras planned and executed 24 oceanographic cruises over a period of 2 years to assess the benthic (SANSED cruise) and pelagic (SANAGU cruise) systems of the SB (356 days at sea in 2019 and 2021/2022). These efforts were part of the Santos Project, which comprised a comprehensive environmental study aimed at investigating benthic and pelagic variables to characterize ecology, biogeochemistry, thermohaline properties of water masses, and ocean circulation patterns, geomorphology, and sedimentology, as well as organic and inorganic chemistry. Here we present the detailed sampling designs and the field methods employed on board, during the SB scientific cruises. All sampling protocols were based on standardized approaches. For the benthos analyses, triplicate sediment samples were performed using a GOMEX-type box corer (0.25 m²) or a large modified Van Veen grab (0.75 m²) at 100 stations ranging from 25 to 2400 m depth. At each station, 25 geochemical and physico-chemical parameters were analyzed in addition to micro-, meio-, and macrofauna and living foraminifera samples. For the pelagic system, 60 stations were selected to investigate the plankton community, ranging in size from pico- to macroplankton, through vertical, horizontal, and oblique net hauls (20, 200, and 500 μm mesh size), as well as 25 biogeochemical parameters collected with an aid of a CTD-rosette sampler. Part of this scientific information also serves the Regional Environmental Characterization Project (PCR-BS) in support of Petrobras’ Santos Basin drilling licensing process led by the Brazilian Environmental Agency – IBAMA. This project contributes to the sustainable development of the SB, in line with the guidelines of the United Nations Decade of Ocean Science for Sustainable Development.

https://doi.org/10.1590/2675-2824071.22072dlm

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