Abstract Background Retinopathy of Prematurity (ROP) is a proliferative retinal vascular disease occurring in the retina of premature infants and is the main cause of childhood blindness. Nowadays anti-VEGF and retinal photocoagulation are mainstream treatments for ROP, but they develop a variety of complications. Hydrogen (H2) is widely considered as a useful neuroprotective and antioxidative therapeutic method for hypoxic-ischemic disease without toxic effects. However, whether H2 provides physiological angiogenesis promotion, neovascularization suppression and glial protection in the progression of ROP is largely unknown. This study aims to investigate the effects of H2 on retinal angiogenesis, neovascularization and neuroglial dysfunction in the retinas of oxygen-induced retinopathy (OIR) mice. Methods In this study, mice that were seven days old and either wild-type (WT) or Nrf2-deficient (Nrf2-/-) were exposed to 75% oxygen for 5 days and then returned to normal air conditions. Different stages of hydrogen gas (H2) inhalation were administered. Vascular obliteration, neovascularization, and blood vessel leakage were analyzed and compared. To count the number of neovascularization endothelial nuclei, routine HE staining of retinal sections was conducted. Immunohistochemistry was performed using DyLight 594 labeled GSL I-isolectin B4 (IB4), as well as primary antibodies against proliferating cell nuclear antigen (PCNA), glial fibrillary acidic protein (GFAP), and Iba-1. Western blots were used to measure the expression of NF-E2-related factor 2 (Nrf2), vascular endothelial growth factor (VEGF), Notch1, Dll4, and HIF-1α. Additionally, the expression of target genes such as NQO1, HO-1, Notch1, Hey1, Hey2, and Dll4 was measured. Human umbilical vein endothelial cells (HUVECs) treated with H2 under hypoxia were used as an in vitro model. RT-PCR was used to evaluate the mRNA expression of Nrf2, Notch/Dll4, and the target genes. The expression of reactive oxygen species (ROS) was observed using immunofluorescence staining. Results Our results indicate that 3–4% H2 does not disturb retinal physiological angiogenesis, but ameliorates vaso-obliteration and neovascularization in OIR mice. Moreover, H2 prevents the decreased density and reverses the morphologic and functional changes in retinal astrocytes caused by oxygen-induced injury. In addition, H2 inhalation reduces microglial activation, especially in the area of neovascularization in OIR mice. H2 plays a protective role in vascular regeneration by promoting Nrf2 activation and suppressing the Dll4-induced Notch signaling pathway in vivo. Also, H2 promotes the proliferation of HUVECs under hypoxia by negatively regulating the Dll4/Notch pathway and reducing ROS levels through Nrf2 pathway aligning with our findings in vivo. Moreover, the retinal oxygen-sensing mechanisms (HIF-1α/VEGF) are also involved in hydrogen-mediated retinal revascularization and neovascularization suppression. Conclusions Collectively, our results indicate that H2 could be a promising therapeutic agent for POR treatment and that its beneficial effect in human ROP might involve the activation of the Nrf2-Notch axis as well as HIF-1α/VEGF pathways.

Abstract Background: Cell Migration Inducing Hyaluronidase 1 (CEMIP) is a protein that plays regulatory functions in a variety of cellular processes in many diseases. Nevertheless, its role and molecular mechanism in keloid hyperplasia are still elusive. Methods: Expressions of CEMIP and Secreted Protein acidic and Rich in Cysteine (SPARC) were detected by qRT-PCR and western blot. CCK-8 assay, along with immunofluorescence staining, was applied for the assessment of cell proliferation. The capabilities of cells to migrate and invade were evaluated utilizing wound healing and Transwell, while Extracellular Matrix (ECM) deposition was measured by immunofluorescence and western blot. The interaction of CEMIP and SPARC was predicted by the Coexpedia and PPA-red databases and verified by co-IP. Western blot was adopted for the estimation of TGF-β/Smad pathway-related proteins. Results: The data demonstrated that CEMIP expression was elevated in Keloid Fibroblasts (KF). CEMIP interference suppressed cell proliferative, migrative and invasive capabilities and ECM deposition in KF. Mechanistically, bioinformatics analysis revealed that CEMIP was co-expressed with SPARC and CEMIP protein could bind to SPARC. SPARC expression was reduced in CEMIP-silenced cells. SPARC overexpression counteracted the impacts of CEMIP silencing on cell proliferative, migrative and invasive capabilities and ECM deposition in KF. In addition, the expressions of TGF-β/Smad signaling-related proteins were decreased by CEMIP silencing via the inhibition of SPARC. Conclusion: In summary, this study revealed that CEMIP modulated KF proliferation, migration, invasion and ECM deposition by TGF-β/Smad signaling through binding to SPARC. Background (CEMIP diseases Nevertheless elusive Methods (SPARC qRTPCR qRT PCR CCK8 CCK 8 CCK- assay staining proliferation Transwell (ECM PPAred PPA red coIP. coIP co IP. IP co-IP TGFβ/Smad TGFβSmad TGF β/Smad β Smad pathwayrelated pathway related Results . (KF) proliferative Mechanistically coexpressed expressed CEMIPsilenced silenced addition signalingrelated Conclusion summary migration TGFβ βSmad (KF

ABSTRACT Inhaling dust can lead to respiratory diseases, and dust accumulation in the workplace can pose fire and explosion hazards. Traditional dust removal nozzles require high water pressure and produce large droplet diameters. The Laval nozzle, utilizing a converging-diverging section to accelerate fluid to supersonic speeds, achieves finer droplets and a more concentrated particle size distribution. However, curved Laval nozzle is different to manufacture. To study the effect of water pressure on the atomization performance of a Linear Laval nozzle, a laser particle analyzer and a camera were used to test the droplet size and atomization angle. These results were compared with numerical analysis. The findings indicate that as the water pressure increases from 0.1 MPa to 0.5 MPa, the dropletsʼ Sauter Mean Diameter (SMD) increases almost linearly. At the same time, the spray angle tends to decrease. Both experimental and numerical analyses show the same trend. At a water pressure of 0.1 MPa, the atomization performance of the Linear Laval nozzle is optimal. Compared to traditional nozzles, the water pressure is significantly reduced, and the D(3,2) droplet diameter is notably smaller. Moreover, the atomization angle is considerably increased. The spray effect has been significantly improved. diseases hazards diameters convergingdiverging converging diverging speeds distribution However manufacture analysis 01 0 1 0. 05 5 SMD (SMD linearly time decrease trend optimal reduced D3,2 D32 D 3,2 3 2 D(3,2 smaller Moreover increased improved D3 D3, 32 3, D(3, D(3 D(

ABSTRACT For the classification and zoning study of crops at Jianshan Farm, data were acquired by integrating Sentinel-2 optical satellite imagery with Sentinel-1 radar satellite imagery. Different crop spectral features were extracted, and four vegetation indices (NDVI, SAVI, EVI, and NDWI) were computed to analyze the spectral differences among soybean, corn, potato, and sorghum. The UNet algorithm was employed to conduct crop zoning on the remote sensing images of Jianshan Farm from September 2020 to September 2023 for four consecutive years. An improved UNet model incorporating a channelwise attention and spatial attention module (CBAM) achieved a prediction accuracy of 100% for the 2023 image data. This represents a 12.5% improvement over the baseline UNet model. These results provide data support for research on crop classification methods. Sentinel2 Sentinel 2 Sentinel- Sentinel1 1 extracted NDVI, NDVI (NDVI SAVI EVI NDWI soybean corn potato sorghum 202 years CBAM (CBAM 100 125 12 5 12.5 methods 20 10 12.

ABSTRACT In soybeans from different years, the infrared spectroscopy waveforms exhibit similarities, yet variations in aging time lead to significant differences in their fatty acid content. To rapidly discern the year of soybean production, this study employed ten feature extraction algorithms in conjunction with Convolutional Neural Networks (CNN) to establish models for classifying soybeans from different years (2019, 2020, and 2021). The research findings reveal the following: (1) The CNN models, after feature extraction, all demonstrated improved accuracy. Notably, the optimal models for both powdered and granulated states were the Kpca-CNN models, achieving an accuracy of 100%. The corresponding loss function values were 0.0002 and 0.0007, with processing times of 0.19 seconds and 0.20 seconds, respectively. (2) The modeling results of all models suggest that the classification accuracy of soybean powder spectral data is higher compared to soybean particle spectral data. (3) Validation of the optimal Kpca-CNN model confirmed its consistent accuracy of 100% when introducing new data or reducing the size of the training dataset. In conclusion, the fusion of near-infrared spectroscopy analysis and CNN technology is considered an effective method for classifying soybeans from different years. This method provides a practical solution for the rapid and precise determination of seed production years.

RESUMO Introdução: O quadro de lesão muscular no treinamento esportivo de esqui tem aumentado gradualmente, prejudicando muito o desempenho das competições esportivas de gelo e neve. Objetivo: Estudar a lesão muscular e o movimento muscular durante o treinamento esportivo no gelo e na neve, bem como a reabilitação das lesões musculares. Métodos: Trinta esquiadores com lesão muscular no joelho foram selecionados como sujeitos e submetidos a treinamento de reabilitação por um total de 6 semanas, tendo os indicadores sido avaliados estatisticamente. Resultados: Os tipos de lesões no esqui foram principalmente tensão muscular, tensão muscular ou ligamentar e ruptura ligamentar. Os índices após o tratamento foram significativamente diferentes daqueles antes do tratamento (P < 0,05); comparado com os três programas de reabilitação, a melhora de cada índice no grupo C foi significativamente diferente da dos outros dois grupos (P < 0,05), enquanto não houve diferença significativa na melhora de cada índice entre o tratamento de treinamento isométrico multiangular no grupo A e a técnica de estimulação neuromuscular proprioceptiva no grupo B (P>0,05). Conclusão: A influência da tecnologia de treinamento de recuperação na reeducação muscular do joelho foi proposta, e foi apresentado um plano de reabilitação para a prática de esqui. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction: Muscle injury in ski sports training has gradually increased, greatly impairing performance in ice and snow sports competitions. Objective: To study muscle injury and muscle movement during ice and snow sports training and the rehabilitation of muscle injuries. Methods: Thirty skiers with knee muscle injuries were selected as subjects and underwent rehabilitation training for six weeks, and the indicators were statistically evaluated. Results: The ski injuries were mainly muscle strain, muscle or ligament strain, and ligament rupture. The indices after treatment were significantly different from those before treatment (P < 0.05); compared with the three rehabilitation programs, the improvement of each index in group C was significantly different from that in the other two groups (P < 0.05), while there was no significant difference in the improvement of each index between the multi-angle isometric training treatment in group A and the proprioceptive neuromuscular stimulation technique in group B (P>0.05). Conclusion: The influence of recovery training technology on knee muscle re-education was proposed, and a rehabilitation plan for skiing was presented. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: El cuadro de lesiones musculares en el entrenamiento de los deportes de esquí ha ido aumentando progresivamente, lo que perjudica en gran medida el rendimiento en las competiciones de deportes de hielo y nieve. Objetivo: Estudiar las lesiones musculares y el movimiento muscular durante el entrenamiento de los deportes de hielo y nieve, así como la rehabilitación de las lesiones musculares. Métodos: Se seleccionaron como sujetos treinta esquiadores con lesiones musculares en la rodilla y se sometieron a un entrenamiento de rehabilitación durante un total de 6 semanas, y se evaluaron estadísticamente los indicadores. Resultados: Los tipos de lesiones de esquí fueron principalmente la distensión muscular, la distensión muscular o de ligamentos y la rotura de ligamentos. Los índices después del tratamiento fueron significativamente diferentes de los anteriores (P < 0,05); en comparación con los tres programas de rehabilitación, la mejora de cada índice en el grupo C fue significativamente diferente de la de los otros dos grupos (P < 0,05), mientras que no hubo diferencias significativas en la mejora de cada índice entre el tratamiento de entrenamiento isométrico multiángulo en el grupo A y la técnica de estimulación neuromuscular propioceptiva en el grupo B (P>0,05). Conclusión: Se propuso la influencia de la tecnología de entrenamiento de recuperación en la reeducación muscular de la rodilla y se presentó un plan de rehabilitación para el esquí. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

This work presented a new PEG modified calix[4]arene stationary phase (C4A-PEG-2PTSC) for gas chromatographic (GC) separations. The statically coated C4A-PEG-2PTSC column exhibited moderate polarity and column efficiency of 2224 plates m-1 determined by 1-octanol at 120 oC. The results showed that the C4A-PEG-2PTSC column exhibited the good separation performance for various types of analytes containing halogenated benzenes, benzaldehydes, phenols, alcohols, anilines isomers and showed the different retention behavior with the comparative columns. Importantly, it showed the better separation capacity of toluidine and xylidine isomers than commercial HP-35 column. Moreover, it was applied in practical sample analysis to detect the content of isomer impurities. This work exhibited the good application potential of C4A-PEG-2PTSC stationary phase in GC analyses. calix4arene calixarene calix 4 arene C4APEG2PTSC CAPEGPTSC C4A 2PTSC C A PTSC (C4A-PEG-2PTSC (GC separations 222 m1 m 1 m- 1octanol octanol 12 oC benzenes benzaldehydes phenols alcohols columns Importantly HP35 HP 35 HP-3 Moreover impurities analyses APEG CA 22 HP3 3 HP- 2

RESUMO Introdução O alongamento dinâmico é uma forma especial de treinamento. Atualmente, existem poucas pesquisas no meio acadêmico sobre alongamento dinâmico na dança esportiva. Objetivo Explorar o papel do treino funcional de alongamento dinâmico na dança esportiva. Métodos 60 bailarinos esportivos com histórico de lesões no tornozelo foram divididos aleatoriamente em grupo controle e experimental. Todos realizaram um protocolo de treinamento duas vezes por semana, com duração de 45 minutos, por 8 semanas. Ao grupo controle foi adicionado um treino de alongamento dinâmico funcional. Os efeitos foram avaliados pela escala de Cumberland, comparação de estabilidade bilateral e controle de equilíbrio pelo sistema de Perkin. Os dados foram tratados estatisticamente para análise. Resultados Antes do experimento, não houve diferença significativa entre os escores das articulações do tornozelo saudáveis e das articulações do tornozelo lesionadas nos dois grupos (P>0,05). Após 8 semanas de treinamento funcional de alongamento dinâmico, houve diferença significativa entre o grupo experimental e o grupo controle nas articulações do tornozelo lesionadas (P<0,05). Conclusão O treinamento de alongamento dinâmico pode efetivamente melhorar a estabilidade da articulação do tornozelo nos bailarinos esportivos. Concomitantemente, esse método previne efetivamente a ocorrência de lesões na articulação do tornozelo do atleta. Nível de evidência II; Estudos terapêuticos - Investigação de resultados.

ABSTRACT Introduction Dynamic stretching is a particular form of training. Currently, there is little research in academia about dynamic stretching in sports dancing. Objective Explore the role of functional dynamic stretching training in dance sports. Methods 60 sports dancers with a history of ankle injuries were randomly divided into a control and experimental group. All performed a training protocol twice a week, lasting 45 minutes, for eight weeks. A functional dynamic stretching training session was added to the control group. The effects were evaluated by the Cumberland scale, bilateral stability comparison, and balance control by the Perkin system. Data were statistically treated for analysis. Results There was no significant difference between the scores of healthy ankle joints and injured ankle joints in the two groups (P>0.05). After eight weeks of functional dynamic stretching training, there was a significant difference between the experimental and control groups on injured ankle joints (P<0.05). Conclusion Dynamic stretching training can effectively improve ankle joint stability in sports dancers. Concomitantly, this method effectively prevents injuries to the athlete’s ankle joint. Evidence level II; Therapeutic Studies - Investigating the results.

RESUMEN Introducción El estiramiento dinámico es una forma especial de entrenamiento. Actualmente, existen pocas investigaciones en el ámbito académico sobre los estiramientos dinámicos en el baile deportivo. Objetivo Explorar el papel del entrenamiento funcional de estiramiento dinámico en el baile deportivo. Métodos 60 bailarines deportivos con antecedentes de lesiones de tobillo fueron divididos aleatoriamente en un grupo de control y otro experimental. Todos realizaron un protocolo de entrenamiento dos veces por semana, de 45 minutos, durante 8 semanas. Al grupo de control se le añadió un entrenamiento de estiramiento dinámico funcional. Los efectos fueron evaluados por la escala Cumberland, la comparación de la estabilidad bilateral y el control del equilibrio por el sistema Perkin. Los datos fueron tratados estadísticamente para su análisis. Resultados Antes del experimento, no había diferencias significativas entre las puntuaciones de las articulaciones del tobillo sano y las articulaciones del tobillo lesionado en los dos grupos (P>0,05). Después de 8 semanas de entrenamiento funcional de estiramiento dinámico, hubo una diferencia significativa entre el grupo experimental y el grupo de control en las articulaciones del tobillo lesionadas (P<0,05). Conclusión El entrenamiento de estiramiento dinámico puede mejorar eficazmente la estabilidad de la articulación del tobillo en los bailarines deportivos. Al mismo tiempo, este método previene eficazmente la aparición de lesiones en la articulación del tobillo del deportista. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Resumen: Este estudio introduce el equilibrio de Stackelberg-Nash en la teoría neoclásica del crecimiento. Intenta hacer que la teoría neoclásica del crecimiento económico sea más robusta en el modelado de la comple- jidad de las estructuras de mercado. El modelo de Solow-Uzawa se construye en el marco dos sectores. La economía se compone de dos sectores: el sector de bienes finales es el mismo que en el modelo de crecimiento de un solo sector de Solow, caracterizado por una competencia perfecta. El sector de bienes de consumo es el mismo que en el modelo de Uzawa, pero se caracteriza por el duopolio de Stackelberg. Se modela el comportamiento de los hogares con el concepto de Zhang de ingresos disponibles y utilidad. El modelo determina endógenamente los beneficios del duopolio que se distribuyen equitativamente entre la población homogénea. Se construye el modelo y luego se identifica la existencia de un punto de equilibrio a través de la simulación. Se realizan análisis estático-comparativos de algunos parámetros. También, se compara el rendimiento económico del modelo tradicional de Uzawa y el modelo con el equilibrio de Stackelberg-Nash. Se concluye que la competencia imperfecta aumenta la producción nacional, la riqueza nacional y el nivel de utilidad en comparación con la competencia perfecta.

Abstract: This study introduces Stackelberg-Nash equilibrium to neoclassical growth theory. It attempts to make neoclassical economic growth theory more robust in modelling the complexity of market structures. The model is constructed within the framework of the Solow-Uzawa two-sector model. The economy is composed of two sectors. The final goods sector is the same as in the Solow one-sector growth model which is characterized by perfect competition. The consumer goods sector is the same as the consumer goods sector in the Uzawa model but is characterized by Stackelberg duopoly. We model household behavior with Zhang’s concept of disposable income and utility. The model endogenously determines profits of duopoly which are equally distributed among the homogeneous population. We build the model and then identify the existence of an equilibrium point through simulation. We conduct comparative static analyses of some parameters. We also compare the economic performance of the traditional Uzawa model and the model with the Stackelberg-Nash equilibrium. We conclude that the imperfect competition increases national output, national wealth, and utility level in comparison to perfect competition. JEL Classification: D43, L13, B40.

Résumé : Cette étude introduit l’équilibre de Stackelberg-Nash dans la théorie néoclassique de la croissance. Elle tente de rendre la théorie néoclassique de la croissance économique plus robuste dans la modélisation de la complexité des structures du marché. Le modèle Solow-Uzawa est construit dans le cadre de deux secteurs. L’économie est composée de deux secteurs. Le secteur des biens finaux est le même que dans le modèle de croissance d’un secteur unique de Solow, caractérisé par une concurrence parfaite. Le secteur des biens de consommation est le même que le secteur des biens de consommation dans le modèle d’Uzawa, mais se caractérise par le duopole de Stackelberg. Nous avons modélisé le comportement des ménages avec le concept de Zhang de revenu disponible et de bénéfices. Le modèle détermine de manière endogène les avantages du duopole qui sont répartis équitablement au sein de la population homogène. Nous construisons le modèle, puis identifions l’existence d’un point d’équilibre par simulation. Nous effectuons des analyses statiques-comparatives de certains paramètres. Nous avons également comparé la performance économique du modèle traditionnel d’Uzawa et du modèle avec l’équilibre de Stackelberg-Nash. Nous concluons qu’une concurrence imparfaite augmente la production nationale, la richesse nationale et le niveau d’utilité par rapport à la concurrence parfaite.

ABSTRACT To solve the poor fitting degree of errors in multiobjective parameter optimization and low accuracy, a multiobjective optimization method based on a BP neural network was proposed. By taking the 1ZT-210 type whole-straw returning device as the research object, a BP neural network model on power consumption, straw returning rate and the influencing factors was obtained. By optimizing the model by the proposed method, the optimal parameter combination of the test factors was as follows: the advancing speed of the device was 0.65 km/h, the blade roll rotating speed was 210 rpm, the blade installation angle was 55o, the minimum power consumption was 9.82 kW and the maximum straw returning rate was 93.23%. Under such test conditions, the minimum power consumption was 10.75 kW, and the straw returning rate was 92.46%, which were all better than those obtained by the regression analysis method. Finally, a verification test was conducted on the results of BP neural network optimization. The power consumption of the test was 10.04 kW, the absolute error was 0.22 kW and the relative error was 2.24%. For a straw returning rate of 93.11%, the absolute error was -0.12% and the relative error was 0.13%. The test results indicated that the optimization method was feasible.

ABSTRACT The parameter optimization of the tractor's steering trapezoid mechanism is a traditional optimization problem, and the teaching-learning-based optimization (TLBO) has a better solving ability for parameter optimization of the tractor's steering trapezoid. However, the teacher stage and student stage of TLBO limit the accuracy and stability and the ability to jump out of the local optimization solution. To obtain an optimal solution with an higher accuracy, an improved adaptive direction strategy teaching-learning-based optimization (IADS-TLBO) was used. This improved the feedback stage based on the adaptive direction strategy teaching-learning-based optimization (ADS-TLBO). The IADS-TLBO was verified by three different testing functions, and the results showed that the improved ideas are valid and feasible. Finally, the IADS-TLBO was used to optimize the steering trapezoid mechanism of JOHN DEERE T600. The optimal parameters obtained were as follows: the bottom angle was 35.4º, and the steering arm length was 154 mm. A verification experiment was conducted in the farm tool laboratory of Northeast Agricultural University (China). The experimental results showed that the average bottom angle was 35.48º, and the relative error between the measured and optimized bottom angles was 0.23%, which is less than 5%. This result showed that the results obtained by IADS-TLBO were reliable.

Abstract To explore and analyze the pain characteristics and countermeasures to improve intraoperative pain for patients with temporomandibular joint disorder during treatment of orthodontics. The patients were randomly divided into control group and the observation group. The control group received routine nursing. It is found in the study that the occurrence of pain in orthodontic treatment is accompanied by certain regular changes, and there is little difference between the proportion of masticatory pain and occlusal pain. In the observation group, the total clinical effective rate increased after nursing (P<0.05. The VAS pain score of the observation group was significantly lower than that of the control group 4h and 8h after nursing intervention was implemented, and the duration of pain symptoms was significantly shorter than that of the control group, with significant statistical difference between the two groups (P<0.05). The targeted nursing intervention for patients with temporomandibular joint disorder undergoing orthodontic treatment based on their characteristics will shorten the pain time of patients effectively and improve the treatment effect, maintaining significant application value.

Abstract Astragaloside IV (AS-IV) has been shown to possess cardioprotective effect. However, the specific mechanism of AS-IV in myocardial infarction (MI) remains unclear. Our results showed that AS-IV intervention observably enhanced cell viability and reduced cell apoptosis, oxidative stress levels and inflammatory factor secretion. Expression of MIRT1 was significantly up-regulated in hypoxia-treated cells. In addition, ALCAM overexpression reversed the effects of AS-IV intervention on hypoxia-treated cell functions. MIRT1 facilitated Runx3 promoter methylation and its downregulated expression by recruiting DNA methyltransferase 3B (DNMT3B) to the Runx3 promoter region through binding with suppressor of Zeste 12 protein homolog (Suz12). Runx3 silencing reversed the effects of MIRT1 inhibition on hypoxia-treated cell functions. Betulinic acid suppressed the effects of AS-IV intervention on the behaviors of hypoxia-treated cells. AS-IV treatment improved the cardiac functions of mice with MI. Taken together, these findings demonstrated that AS-IV treatment inhibits DNMT3B-mediated Runx3 promoter methylation by restraining Suz12 expression and further blocks the NF-κB signaling pathway, and in turn improves the cardiac functions of mice with MI.

Resumo Introdução: Evidências emergentes indicam que as condições fisiológicas e patológicas do nariz são reguladas pós-transcripcionalmente por microRNAs, uma classe de pequenos RNAs não codificantes. Recentemente, o microRNA-223-3p tem sido cada vez mais implicado na modulação da rinite alérgica. Objetivo: Avaliar o papel e o mecanismo do microRNA-223-3p em um modelo de rinite alérgica em camundongos. Método: O nível de expressão do microRNA-223-3p foi medido no soro de 41 pacientes com rinite alérgica e 39 controles saudáveis com polimerase chain reaction quantitativo em tempo real. Camundongos BALB/c foram usados para estabelecer um modelo de rinite alérgica através de sensibilização intraperitoneal e teste de provocação nasal com ovalbumina. O microRNA-223-3p agomir/antagomir foi então administrado por via intranasal a camundongos após provocação com ovabulmina por mais uma semana. Os sintomas de prurido nasal e espirros foram registrados. A concentração sérica de imunoglobulina E específica para ovalbumina, expressão de microRNA-223-3p e níveis de citocinas pró-inflamatórias (IL-4, IL-5, IFN-γ) na mucosa nasal foram medidos por ELISA e polimerase chain reaction quantitativo, respectivamente. As alterações histopatológicas foram avaliadas com coloração com hematoxilina e eosina. Resultados: Os níveis de microRNA-223-3p aumentaram significativamente em ambos os pacientes e camundongos com rinite alérgica. Além disso, a suprarregulação dos níveis de microRNA-223-3p através da administração nasal de microRNA-223-3p agomir também aumentou significativamente a concentração de IgE específica para ovalbumina, as frequências de prurido nasal e espirros, os níveis de citocinas pró-inflamatórias (IL-4, IL-5, IFN-γ) e infiltração de eosinófilos na mucosa nasal de camundongos com rinite alérgica. Além disso, o microRNA-223-3p antagomir pareceu melhorar significativamente os sintomas e a doença da mucosa nasal. Posteriormente, demonstramos pela primeira vez que o microRNA-223-3p regulou negativamente a expressão de INPP4A ao se ligar à região 3’ não traduzida 3'UTR) do gene INPP4A. Conclusões: Esses achados indicam que o microRNA-223-3p desempenha um papel importante na regulação da doença e dos sintomas da rinite alérgica, tem como alvo o gene INPP4A.

Abstract Introduction: Emerging evidence indicates that physiological and pathological conditions of the nose are posttranscriptionally regulated by microRNAs, a class of small noncoding RNAs. Recently, microRNA-223-3p has been increasingly implicated in the modulation of allergic rhinitis Objective: This study aimed to assess the role and mechanism of microRNA-223-3p in a mouse model of allergic rhinitis. Methods: The expression level of miR-223-3p was measured in the serum of 41 allergic rhinitis patients and 39 healthy controls using quantitative real time polymerase chain reaction. BALB/c mice were used to establish an allergic rhinitis model by intraperitoneal sensitization and intranasal challenge with ovalbumin. MicroRNA-223-3p agomir/antagomir was then intranasally administered to mice after ovalbumin challenge for another week. The symptoms of nasal rubbing and sneezing were recorded. Serum ovalbumin-specific immunoglobulin E concentration, microRNA-223-3p expression and proinflammatory cytokine (IL-4, IL-5, IFN-γ) levels in nasal mucosa were measured by ELISA and quantitative real time polymerase chain reaction, respectively. Histopathologic changes were evaluated using hematoxylin and eosin staining. Results: MicroRNA-223-3p levels increased significantly in both allergic rhinitis patients and allergic rhinitis mice. In addition, upregulation of microRNA-223-3p levels by nasal administration of microRNA-223-3p agomir also markedly increased the concentration of ovalbumin -specific IgE, the frequencies of nasal rubbing and sneezing, the levels of proinflammatory cytokines (IL-4, IL-5, IFN-γ) and eosinophil infiltration in the nasal mucosa of allergic rhinitis mice. Moreover, microRNA-223-3p antagomir appeared to strongly ameliorate the symptoms and pathology in nasal mucosa. Subsequently, we demonstrated for the first time that microRNA-2233p negatively regulated INPP4A expression by binding with the 3 untranslated region 3'UTR) of INPP4A. Conclusions: These findings indicate that microRNA-223-3p plays an important role in regulating the pathology and symptoms of allergic rhinitis by targeting INPP4A.

ABSTRACT Protecting against the main sugarcane diseases and insect pests requires good control at the base of the plant; therefore, higher droplet penetration is required during plant protection. The objectives of this research were to explore the movement mechanism of droplets in a sugarcane protection operation using a single-rotor unmanned aerial vehicle (UAV) and establish the connection between the UAV downwash flow field and droplet penetration. By considering the downwash flow field of a single-rotor UAV in layers, a theoretical model of the vertical velocities of droplets was established, and verification experiments were carried out in a sugarcane field. The results showed that the velocity of the UAV downwash flow was the main factor affecting the final vertical velocity of the droplets, the final vertical velocity of a droplet was positively correlated with the droplet diameter, and the initial droplet velocity had no significant effect on the final droplet velocity. In the transportation of droplets, the droplet velocity quickly approached the velocity of the airflow, and the smaller the droplet diameter was, the higher the acceleration. These research results can provide guidance for the theoretical study of droplet deposition effects in sugarcane protection operations using single-rotor UAVs.