Abstract Background Talaromyces Marneffei (TM) is a rare opportunistic pathogen that mostly infects patients with low immunity compared to those with normal immunity. It may be related to immune deficiency or genetic factors. Objective To evaluate the gene mutation of a patient infected with TM in an endemic area with negative anti-interferon-γ autoantibodies, and negative human immunodeficiency virus (HIV) infection. Methods Extract deoxyribonucleic acid (DNA) samples from the patient's peripheral blood, detect the mutation gene by whole exome sequencing (WES), and carry out Sanger sequencing verification for the detected mutation gene. Results The authors detected a mutation in the IFNGR1 gene (NM_001363526.1) and validated the detected gene mutation using Sanger sequencing. The results showed a heterozygous mutation c.4C>T (p.L2F) located in the IFNGR1 gene (NM_001363526.1). Study limitations The mechanism of the IFNGR1 gene has not been further investigated in this study. Conclusions The IFNGR1 gene mutation may be a potential risk factor for TM infection, and the presence of anti-interferon-γ autoantibodies can aggravate disease symptoms. (TM factors antiinterferonγ anti interferon γ HIV (HIV infection DNA (DNA s blood WES, WES , (WES) IFNGR NM_001363526.1 NM0013635261 NM 001363526 1 (NM_001363526.1 c.4C&gtT c4CgtT cCgtT c.4C&gt T c 4C gt C p.L2F pL2F pLF p L2F L F (p.L2F NM_001363526.1. . study symptoms (WES NM_001363526. NM001363526 00136352 (NM_001363526. gtT CgtT c4Cgt cCgt pL LF NM_001363526 NM00136352 0013635 (NM_001363526 Cgt NM_00136352 NM0013635 001363 (NM_00136352 NM_0013635 NM001363 00136 (NM_0013635 NM_001363 NM00136 0013 (NM_001363 NM_00136 NM0013 001 (NM_00136 NM_0013 NM001 00 (NM_0013 NM_001 NM00 0 (NM_001 NM_00 NM0 (NM_00 NM_0 (NM_0 NM_ (NM_ (NM

Abstract Objective: To determine whether peripheral blood parameters have any predictive value for all-frequency Sudden Sensorineural Hearing Loss (SSNHL). Methods: We chose 78 individuals with all-frequency SSNHL who had been admitted to our department. They were divided into two groups: the effective group and the ineffective group. In patients with all-frequency SSNHL, the prognostic variables, including peripheral blood tests and clinical traits, were examined by a logistic regression analysis. In addition, the predictive value was carried out. Results: The effective rate of all-frequency SSNHL was 61.5%. Pre-treatment hearing level and the proportion of patients with diabetes were both significantly lower in the effective group than in the ineffective group (p = 0.024 and 0.000, respectively). The levels of fibrinogen and C-reactive protein were also significantly different between the two groups (p = 0.001 and 0.025, respectively). Pre-treatment hearing level and fibrinogen level both significantly impacted the prognosis of all-frequency SSNHL (p = 0.032 and 0.002, respectively), according to a logistic regression analysis. Furthermore, the prognosis was significantly predicted by both fibrinogen level and pre-treatment hearing level (p = 0.001 and 0.0002, respectively). The receiver operating characteristic curve showed that the fibrinogen level had a sensitivity of 85.4% and a specificity of 60.0% for predicting the prognosis of all-frequency SSNHL. Conclusion: For the prognosis of all-frequency SSNHL, the fibrinogen level can be regarded as a useful predictor. The level of C-reactive protein, however, does not have a significant prognostic effect on predicting all-frequency SSNHL. Therefore, more attention should be devoted to the level of fibrinogen in the acute period of all-frequency SSNHL. Level of Evidence: 4. Objective allfrequency all frequency . (SSNHL) Methods 7 department variables traits analysis addition out Results 615 61 5 61.5% Pretreatment Pre treatment p 0024 0 024 0.02 0000 000 0.000 respectively. respectively respectively) Creactive C reactive 0001 001 0.00 0025 025 0.025 0032 032 0.03 0002 002 0.002 respectively, , Furthermore pretreatment pre 00002 0.0002 854 85 4 85.4 600 60 60.0 Conclusion predictor however Therefore Evidence (SSNHL 6 61.5 02 0.0 00 003 03 8 85. 60. 61. 0.

Abstract Background: Isoliquiritigenin (ISL) presents antitumor effects against melanoma cells. It is known that various circular RNAs (circRNAs) are involved in the development of melanoma. Therefore, the present study aims to investigate the molecular mechanisms of ISL and circ_0002860. Methods: Circ_0002860, microRNA-431-5p (miR-431-5p) and member RAS oncogene family (RAB9A) were detected through reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay. Cell viability was examined via cell counting kit-8 assay. The proliferation ability was assessed using colony formation assay. Cell apoptosis and cell cycle were determined by flow cytometry. Transwell assay was used for detection of migration and invasion. Western blot was conducted for protein analysis. Target binding was confirmed via dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. In vivo research was performed through xenograft tumor assay. Results: Circ_0002860 was downregulated by ISL in melanoma cells. ISL-induced inhibitory effects on cell proliferation, cell cycle progression, migration and invasion were alleviated by circ_0002860 overexpression. MiR-431-5p was a target of circ_0002860. Circ_0002860 eliminated the ISL-induced tumor inhibition via sponging miR-431-5p in melanoma cells. Circ_0002860 elevated the RAB9A level by targeting miR-431-5p. The function of ISL was related to miR-431-5p/RAB9A axis in melanoma progression. Tumor growth was reduced by ISL in vivo through downregulating circ_0002860 to regulate miR-431-5p and RAB9A levels. Conclusion: The current data indicates that ISL suppressed cell malignant progression of melanoma via targeting the circ_0002860/miR-431-5p/RAB9A pathway. Background (ISL cells circRNAs (circRNAs Therefore circ0002860 circ 0002860 Methods Circ0002860 Circ microRNA4315p microRNAp microRNA 431 5p p miR4315p miRp miR (miR-431-5p RABA RAB A (RAB9A transcriptionquantitative transcription quantitative RTqPCR RT qPCR (RT-qPCR kit8 kit 8 kit- cytometry analysis dualluciferase dual luciferase RIP (RIP Results Circ_000286 ISLinduced induced circ_000286 overexpression MiR4315p MiRp MiR miR4315p. 5p. miR4315p/RAB9A miR4315pRAB9A miRpRABA 5p/RAB9A levels Conclusion circ_0002860/miR4315p/RAB9A circ0002860miR4315pRAB9A circmiRpRABA circ_0002860/miR pathway circ000286 000286 Circ000286 43 Circ_00028 circ_00028 pRAB 5pRAB9A pRABA circ0002860miR circmiR circ00028 00028 Circ00028 4 Circ_0002 circ_0002 circ0002 0002 Circ0002 Circ_000 circ_000 circ000 000 Circ000 Circ_00 circ_00 circ00 00 Circ00 Circ_0 circ_0 circ0 0 Circ0 Circ_ circ_

RESUMEN Introducción: En la actualidad, el entrenamiento compuesto de estiramiento rápido ha sido explorado por los investigadores deportivos, sin embargo, la mayoría de las investigaciones se centran en el fútbol, el baloncesto, el voleibol y otros deportes que requieren la aptitud de salto vertical, sin embargo, se presta poca atención al bádminton. Objetivo: Estudiar la influencia del entrenamiento de estiramientos rápidos compuestos en la condición física de los atletas profesionales de bádminton. Métodos: Mediante métodos experimentales y estadísticos, un total de 16 destacados jugadores universitarios de bádminton fueron divididos aleatoriamente en grupos de entrenamiento compuesto y de control. La prueba de rendimiento deportivo (carrera en forma de T) y la prueba de movilidad en distancias cortas (5-10-5), fueron evaluadas y comparadas antes y después de la intervención, que consistió en añadir un protocolo específico de entrenamiento de estiramiento rápido al grupo compuesto. Resultados: Comparativamente, las pruebas de carrera en T y de salto hexagonal mejoraron significativamente en el grupo compuesto (P<0,01). El tiempo requerido para completar la prueba de movimiento 5-10-5 pasó de 3,980s a 3,763s, lo que fue significativamente mayor que el del grupo de control (P<0,05). Conclusión: Se demostró que un entrenamiento de 6 semanas con estiramientos rápidos mejora significativamente la forma física de los atletas universitarios de bádminton. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción actualidad deportivos embargo fútbol baloncesto vertical Objetivo Métodos estadísticos 1 5105, 5105 5 10 , (5-10-5) intervención Resultados Comparativamente P<0,01. P001 P P<0,01 . 0 01 (P<0,01) 5-10- 3980s s 3 980s 3763s 763s 3,763s P<0,05. P005 P<0,05 05 (P<0,05) Conclusión II tratamiento 510 (5-10-5 P00 P<0,0 (P<0,01 5-10 (P<0,05 51 (5-10- P0 P<0, (P<0,0 5-1 (5-10 P<0 (P<0, 5- (5-1 P< (P<0 (5- (P< (5 (P (

ABSTRACT Introduction: Currently, compound rapid stretching training has been explored by sports researchers. However, most research focuses on soccer, basketball, volleyball, and other sports that require vertical jump fitness, but little attention is paid to badminton. Objective: Study the influence of compound rapid stretching training on fitness in professional badminton athletes. Methods: By experimental and statistical methods, 16 outstanding college badminton players were randomly divided into composite training and control groups. The sports performance test (T-shaped run) and short-distance mobility test (5-10-5), were evaluated and compared before and after the intervention, adding a specific rapid stretching training protocol to the composite group. Results: Comparatively, the T-run and hexagonal jump tests were significantly improved in the composite group (P<0.01). The time required to complete the 5-10-5 moving test progressed from 3.980s to 3.763s, significantly longer than the control group (P<0.05). Conclusion: 6-week training with rapid stretching was shown to significantly improve the fitness of college badminton athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction Currently researchers However soccer basketball volleyball Objective athletes Methods methods 1 groups Tshaped T shaped run shortdistance short distance 5105, 5105 5 10 , (5-10-5) intervention Results Comparatively Trun P<0.01. P001 P P<0.01 . 0 01 (P<0.01) 5-10- 3980s s 3 980s 3763s 763s 3.763s P<0.05. P005 P<0.05 05 (P<0.05) Conclusion 6week week 6 II outcomes 510 (5-10-5 P00 P<0.0 (P<0.01 5-10 (P<0.05 51 (5-10- P0 P<0. (P<0.0 5-1 (5-10 P<0 (P<0. 5- (5-1 P< (P<0 (5- (P< (5 (P (

RESUMO Introdução: Atualmente, o treinamento composto de alongamento rápido tem sido explorado por pesquisadores esportivos, no entanto, a maioria das pesquisas se concentra no futebol, basquete, vôlei e outros esportes que exigem aptidão física de salto vertical, porém pouca atenção é dada ao badminton. Objetivo: Estudar a influência do treinamento composto de alongamento rápido sobre a aptidão física nos atletas profissionais de badminton. Métodos: Pelos métodos experimental e estatístico, um total de 16 jogadores de badminton universitários de destaque foram divididos aleatoriamente em grupos de treinamento composto e controle. O teste de desempenho esportivo (corrida em forma de T) e de mobilidade de curta distância (5-10-5), foram avaliados e comparados antes e após a intervenção, que consistiu na adição de um protocolo específico de treinamento de alongamento rápido ao grupo composto. Resultados: Comparativamente, os testes de corrida em T e de salto hexagonal foram significativamente aprimorados no grupo composto (P<0,01). O tempo necessário para completar o teste móvel de 5-10-5 progrediu de 3.980s para 3.763s, o que foi significativamente maior que o do grupo controle (P<0.05). Conclusão: O treinamento de 6 semanas com alongamento rápido demonstrou melhorar significativamente a aptidão física dos atletas universitários de badminton. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução Atualmente esportivos entanto futebol basquete vertical Objetivo Métodos estatístico 1 5105, 5105 5 10 , (5-10-5) intervenção Resultados Comparativamente P<0,01. P001 P P<0,01 . 0 01 (P<0,01) 5-10- 3980s s 3 980s 3763s 763s 3.763s P<0.05. P005 P<0.05 05 (P<0.05) Conclusão II tratamento 510 (5-10-5 P00 P<0,0 (P<0,01 5-10 P<0.0 (P<0.05 51 (5-10- P0 P<0, (P<0,0 5-1 P<0. (P<0.0 (5-10 P<0 (P<0, 5- (P<0. (5-1 P< (P<0 (5- (P< (5 (P (

RESUMEN Introducción: El ejercicio de la fuerza funcional en los velocistas puede suponer una ganancia de rendimiento esencial para la propulsión. El entrenamiento funcional puede afectar significativamente al velocista, tanto en la prevención de lesiones como en el mejor aprovechamiento del entrenamiento físico. El método de entrenamiento por intervalos de sprint ha sido ampliamente reconocido y aplicado. Objetivo: Este artículo explora el efecto del entrenamiento de fuerza funcional mediante el método de entrenamiento de intervalos de sprint en la calidad de la fuerza de los velocistas. Métodos: Este artículo utiliza un muestreo aleatorio para seleccionar 30 velocistas universitarios como sujetos de investigación. Los voluntarios se dividieron en dos grupos según el rendimiento del proyecto de velocidad de 100 metros: Todos los datos básicos de los sujetos que superaron el test-T fueron sin diferencia estadística P>0,05. Ambos grupos realizan un entrenamiento diario. Al grupo experimental se le añadió el entrenamiento de fuerza funcional por intervalos de sprint con un ciclo completo de 12 semanas. Los datos de fuerza de los atletas se midieron antes y después del experimento, y se analizaron estadísticamente. Resultados: Los indicadores mostraron diferencias significativas, a excepción de la prueba normal de apoyo a la posición supina izquierda, no modificada por la intervención presentada (P<0,01). Tras el experimento, todos los indicadores fueron estadísticamente significativos en comparación con el grupo de control (P<0,01). El rendimiento de los grupos en el ejercicio con balón, el salto estático y el salto vertical mejoró significativamente (P<0,01). Conclusión: El ejercicio de fuerza funcional puede mejorar el control corporal y la estabilidad de los velocistas. El método de entrenamiento por intervalos de sprint también puede mejorar la velocidad de transmisión de la fuerza ascendente. Esta conclusión es significativa para la formulación en el plan de entrenamiento de fuerza para los velocistas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción propulsión velocista físico aplicado Objetivo Métodos 3 10 metros testT test T P005 P 0 05 P>0,05 diario 1 semanas experimento Resultados significativas izquierda P<0,01. P001 P<0,01 . 01 (P<0,01) balón Conclusión ascendente II tratamiento P00 P>0,0 P<0,0 (P<0,01 P0 P>0, P<0, (P<0,0 P>0 P<0 (P<0, P> P< (P<0 (P< (P

ABSTRACT Introduction: The exercise of functional strength in a sprinter can play an essential performance gain to propulsion. Functional training can significantly affect the sprinter, preventing injury and making better use of physical training. The sprint interval training method has been widely recognized and applied. Objective: This paper explores the effect of functional strength training by the sprint interval training method on the strength quality of sprinters. Methods: This paper uses random sampling to select 30 male college sprinters as research subjects. The volunteers were divided into two groups according to the performance of the 100-meter speed project: All the essential data of the subjects who passed the T-test were without statistical difference P>0.05. Both groups perform daily training. Functional strength training was added to the experimental group by interval start training with a full 12-week cycle. The strength data of the athletes were measured before and after the experiment, measured, and discussed statistically. Results: The indicators showed significant differences, except for the normal left supine position support test, unchanged by the presented intervention(P<0.01). After the experiment, all indicators were statistically significant compared to the control group (P<0.01). The performance of the groups in the ball exercise, static jump, and vertical jump was significantly improved (P<0.01). Conclusion: Functional strength exercise can improve sprinters’ body control and stability. The sprint interval method can also improve the speed of upward force transmission. This conclusion is significant for formulating the strength training plan designed for sprinters. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction propulsion applied Objective Methods 3 100meter meter 100 project Ttest T test P005 P 0 05 P>0.05 12week week 12 cycle experiment Results differences interventionP<0.01. interventionP001 interventionP intervention P<0.01 . 01 intervention(P<0.01) P<0.01. P001 (P<0.01) Conclusion stability transmission II outcomes 10 P00 P>0.0 1 interventionP<0.01 interventionP00 P<0.0 intervention(P<0.01 (P<0.01 P0 P>0. interventionP<0.0 interventionP0 P<0. intervention(P<0.0 (P<0.0 P>0 interventionP<0. P<0 intervention(P<0. (P<0. P> interventionP<0 P< intervention(P<0 (P<0 interventionP< intervention(P< (P< intervention(P (P

RESUMO Introdução: O exercício da força funcional no velocismo pode desempenhar um ganho de performance essencial à propulsão. O treinamento funcional pode afetar significativamente o velocista, tanto na prevenção de lesões quanto ao melhor aproveitamento do treinamento físico. O método de treinamento intervalado de arranque tem sido amplamente reconhecido e aplicado. Objetivo: Este artigo explora o efeito do treinamento de força funcional pelo método de treinamento intervalado de arranque sobre a qualidade da força dos velocistas. Métodos: Este artigo usa amostragem aleatória para selecionar 30 velocistas universitários masculinos como objetos de pesquisa. Os voluntários foram divididos em dois grupos de acordo com o desempenho do projeto de 100 metros de velocidade: Todos os dados essenciais dos sujeitos que passaram no teste T foram sem diferença estatística P>0,05. Ambos os grupos realizam um treinamento diário. Ao grupo experimental foi acrescentado o treinamento de força funcional pelo treinamento intervalado de arranque com ciclo completo de 12 semanas. Os dados de força dos atletas foram mensurados antes e após o experimento, mensurados e discutidos estatisticamente. Resultados: Os indicadores demonstraram diferenças significativas, exceto para o teste normal de apoio à posição supina esquerda, inalterado pela intervenção apresentada (P<0,01). Após o experimento, todos os indicadores foram estatisticamente significativos em comparação com o grupo controle (P<0,01). O desempenho dos grupos em exercício de bola, salto estático e salto vertical foram significativamente aprimorados (P<0,01). Conclusão: O exercício de força funcional pode melhorar o controle e a estabilidade corporal dos velocistas. O método de treinamento intervalado de arranque também pode melhorar a velocidade de transmissão de força ascendente. Essa conclusão é significativa para a formulação no plano de treinamento de força destinado à velocistas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução propulsão velocista físico aplicado Objetivo Métodos 3 pesquisa 10 P005 P 0 05 P>0,05 diário 1 semanas experimento Resultados significativas esquerda P<0,01. P001 P<0,01 . 01 (P<0,01) bola Conclusão ascendente II tratamento P00 P>0,0 P<0,0 (P<0,01 P0 P>0, P<0, (P<0,0 P>0 P<0 (P<0, P> P< (P<0 (P< (P

Abstract Objective Osteoarthritis is a condition characterized by articular cartilage degradation. The increased expression of β1,4-Galactosyltransferase-I (β1,4-GalT-I) in the articular cartilage of osteoarthritis patients was related to an inflammatory response. The aim of this study was to elucidate the role of β1,4-GalT-I in osteoarthritis. This study aimed to determine the function of 1,4-GalT-I in osteoarthritis. Methods The osteoarthritis mouse model with the destabilization of the medial meniscus was established by microsurgical technique. Pathological changes in articular cartilage were observed by hematoxylin and eosin staining and safranin O-fast green staining. Quantitative real-time polymerase chain reaction, western blot, and enzyme-linked immunosorbent assays were used to observe mRNA and protein expression, respectively. RNA interactions were verified by a luciferase reporter assay. SA-β-Gal staining was used to assess chondrocyte senescence. Immunofluorescence staining was conducted to observe the localization of Nuclear Factor-kappaB (NF-κB). Results β1,4-GalT-I and microRNA-15a (miR-15a) show high and low expression in the articular cartilage of osteoarthritis, respectively. MiR-15a inhibits the mRNA translation of β1,4-GalT-I. β1,4-GalT-I promotes extracellular matrix degradation, senescence, and NF-κB activation in IL-1β-stimulated chondrocytes, which can be reversed by overexpression of miR-15a. Intra-articular injection of microRNA-15a ameliorates cartilage degeneration by inhibiting β1,4-GalT-I and phosphorylation of NF-κB in vivo. Conclusion The authors clarified that the miR-15a/β1,4-GalT-I axis inhibits the phosphorylation of NF-κB thereby inhibiting extracellular matrix degradation and senescence in chondrocytes to alleviate cartilage degeneration in osteoarthritis. MiR-15a and β1,4-GalT-I may serve as potentially effective targets for the future treatment of osteoarthritis. β1,4GalactosyltransferaseI β14GalactosyltransferaseI βGalactosyltransferaseI β1,4 Galactosyltransferase I β1 4 β β1,4GalTI β14GalTI βGalTI GalT (β1,4-GalT-I response 1,4GalTI 14GalTI GalTI 1,4 1 technique Ofast O fast realtime real time reaction blot enzymelinked enzyme linked respectively assay SAβGal SA Gal FactorkappaB Factor kappaB NFκB. NFκB NF κB . (NF-κB) microRNA15a microRNAa microRNA 15a miR15a miRa miR (miR-15a MiR15a MiRa MiR β1,4GalTI. I. IL1βstimulated ILβstimulated IL 1β stimulated miR15a. 15a. miR-15a Intraarticular Intra vivo miR15a/β1,4GalTI miR15aβ14GalTI miRaβGalTI 15a/β1,4 4GalactosyltransferaseI GalactosyltransferaseI β14 β1, 4GalTI 14 1, (NF-κB βstimulated aβ 15aβ14 15a/β1, 15aβ1 15a/β1 15aβ 15a/β

RESUMEN Introducción: La condición física de los futbolistas en las competiciones deportivas ha ido mejorando a lo largo de los años y el rendimiento óptimo de sus habilidades profesionales en condiciones competitivas se ha convertido en un factor esencial para la victoria en los partidos de fútbol. Objetivo: Este trabajo explora los efectos de diferentes métodos que emplean el entrenamiento de la fuerza en las técnicas de pateo de fútbol con el objetivo de encontrar el conjunto que mejor permita la precisión de los golpes a través de su estabilidad. Métodos: Se dividieron aleatoriamente 36 jugadores de fútbol, sin diferencias estadísticas en cuanto a la aptitud física y las características de capacidad integral de los jugadores, en grupo experimental y grupo de control. Ambos grupos de sujetos fueron entrenados durante 12 semanas, sólo el grupo experimental recibió la intervención especial de entrenamiento de fuerza para la estabilidad. Se midió la puntuación del pase de la pelota curva en el regateo de 20 metros antes y después del entrenamiento. Los datos fueron tratados estadísticamente. Resultados: La precisión de las patadas de los futbolistas del grupo experimental fue diferente a la de antes de la prueba (P<0,01). También hubo una diferencia significativa en la precisión de las patadas en el grupo de control (P<0,05). Las puntuaciones del regateo en arco de 20 metros en el grupo experimental fueron estadísticamente significativas en comparación con las anteriores a la prueba (P<0,05). No hubo diferencias significativas entre el grupo de control y las puntuaciones de la prueba en las puntuaciones de los pases de balón curvo en el regateo de 20 metros (P>0,05). Conclusión: Se han desarrollado métodos de fuerza funcional para lograr el objetivo de mejorar la precisión de las patadas en los atletas. Los entrenadores deben prestar atención a la preparación física, una actitud que anima a los jugadores a conseguir la fuerza física suficiente para los partidos de fútbol con sus habilidades de pateo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción Objetivo estabilidad Métodos 3 1 semanas 2 Resultados P<0,01. P001 P P<0,01 . 0 01 (P<0,01) P<0,05. P005 P<0,05 05 (P<0,05) P>0,05. P>0,05 (P>0,05) Conclusión atletas II tratamiento P00 P<0,0 (P<0,01 (P<0,05 P>0,0 (P>0,05 P0 P<0, (P<0,0 P>0, (P>0,0 P<0 (P<0, P>0 (P>0, P< (P<0 P> (P>0 (P< (P> (P

ABSTRACT Introduction: The physical condition of soccer players in sports competitions has improved over the years. The optimal performance of their professional skills in competitive conditions has become essential for victory in soccer matches. Objective: This paper explores the effects of different methods employing strength training on soccer kicking techniques aiming at the set that best enables the accuracy of hits through its stability. Methods: 36 soccer players were randomly divided into the experimental and control groups, with no statistical difference in fitness and the comprehensive ability characteristics of the players. Both subjects were trained for 12 weeks; only the experimental group received the special strength training intervention for stability. The passing score of the curve ball in 20-meter dribbling was measured before and after training. The data were statistically treated. Results: The kicking accuracy of soccer players in the experimental group differed from before the test (P<0.01). There was also a significant difference in kicking accuracy in the control group (P<0.05). The 20-meter arc dribbling scores in the experimental group were statistically significant compared to those before the test (P<0.05). There was no significant difference between the control group and the test scores on curve ball passing scores in 20-meter dribbling (P>0.05). Conclusion: Functional strength methods to achieve the goal of improving kicking accuracy in athletes have been developed. Coaches should pay attention to physical training, an attitude that encourages players to achieve sufficient physical strength for soccer games with their kicking skills. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction years matches Objective stability Methods 3 groups 1 weeks 20meter meter 20 treated Results P<0.01. P001 P P<0.01 . 0 01 (P<0.01) P<0.05. P005 P<0.05 05 (P<0.05) P>0.05. P>0.05 (P>0.05) Conclusion developed II outcomes 2 P00 P<0.0 (P<0.01 (P<0.05 P>0.0 (P>0.05 P0 P<0. (P<0.0 P>0. (P>0.0 P<0 (P<0. P>0 (P>0. P< (P<0 P> (P>0 (P< (P> (P

RESUMO Introdução: A condição física dos jogadores de futebol nas competições esportivas tem se aprimorado ao longo dos anos e o desempenho ótimo de suas habilidades profissionais em condições competitivas tornou-se um fator essencial para a vitória nas partidas de futebol. Objetivo: Este artigo explora os efeitos de diferentes métodos empregando o treinamento de força sobre as técnicas de chute de futebol visando o conjunto que melhor capacita a precisão de acertos através de sua estabilidade. Métodos: 36 jogadores de futebol foram divididos aleatoriamente, sem diferença estatística de aptidão física e as características de capacidade abrangente dos jogadores, em grupo experimental e controle. Ambos os grupos de sujeitos foram treinados durante 12 semanas, somente o grupo experimental recebeu a intervenção especial de treinamento de força para estabilidade. A pontuação de passe da bola curva em drible de 20 metros foi medida antes e depois do treino. Os dados foram tratados estatisticamente. Resultados: A precisão do chute dos jogadores de futebol no grupo experimental foi diferente daquela antes do teste (P<0,01). Também houve uma diferença significativa na precisão de chutes no grupo de controle (P<0,05). As pontuações dos dribles de arco de 20 metros no grupo experimental foram estatisticamente significativas em comparação com aquelas antes do teste (P<0,05). Não houve diferença significativa entre o grupo de controle e os resultados do teste na pontuação de passe da bola curva em drible de 20 metros (P>0,05). Conclusão: Métodos de força funcional para alcançar o objetivo de melhorar a precisão de chute nos atletas foram desenvolvidos. Os treinadores devem prestar atenção ao treinamento físico, atitude que incentiva aos jogadores atingirem a força física suficiente para os jogos de futebol com suas habilidades de chute. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução tornouse tornou Objetivo estabilidade 3 aleatoriamente 1 semanas 2 treino Resultados P<0,01. P001 P P<0,01 . 0 01 (P<0,01) P<0,05. P005 P<0,05 05 (P<0,05) P>0,05. P>0,05 (P>0,05) Conclusão desenvolvidos físico II tratamento P00 P<0,0 (P<0,01 (P<0,05 P>0,0 (P>0,05 P0 P<0, (P<0,0 P>0, (P>0,0 P<0 (P<0, P>0 (P>0, P< (P<0 P> (P>0 (P< (P> (P

Abstract To investigate the effect of corosolic acid treatment on erectile function in metabolic syndrome induced rat model. Fifty male 3-week-old SD rats were fed a high fat and high sugar diet. Six months later, metabolic variables were determined. Metabolic syndrome induced erectile function (MED) rats were confirmed by an apomorphine test. Then MED rats were treated with corosolic acid daily by oral gavage for 4 weeks. To evaluate erectile function, intracavernosal pressure (ICP)/mean arterial blood pressure (MAP) ratio was measured. Thiobarbituric acid reactive substances assay and dihydroethidium staining were used to assess reactive oxygen species (ROS) level. Protein expressions of gp91phox and eNOS were examined by western blotting and immunohistochemistry. Fasting blood glucose, body weight, total cholesterol and insulin were markedly increased in metabolic syndrome rats compared with those of the control rats (p < 0.05). The ratios of max ICP/MAP and area under curve (AUC)/MAP was markedly reduced in MED rats compared with the control rats (p < 0.05). The concentration of cyclic guanosine mono-phosphate (cGMP) and the expression of eNOS were significantly decreased in MED rats compared with the control group (p < 0.05). Moreover, ROS level and the expression of gp91phox were significantly increased in MED rats. Treatment with corosolic acid reversed these changes (each p < 0.05). Corosolic acid reduces the level of ROS, ameliorating endothelial dysfunction and improvement of erectile function in MED rats.

Abstract To investigate the function and mechanism of GINS complex subunit 2 (GINS2) in osteoblast and osteoclast in steroid-induced osteonecrosis of the femoral head (SINFH). Clinical specimens were collected from 40 cases of SINFH patients and 40 cases of control patients. Then, SINFH mouse model was built. Immediately, osteoblasts and osteoclasts were induced and differented from the pre-osteoblast cell line MC3T3-E1 and the mononuclear macrophage cell line RAW264.7 of mice, respectively. The qRT-PCR assay was used for detecting the expressions of GINS2, p53 and growth arrest and DNA damage 45A (GADD45A). The cytoactive, proliferation and apoptosis of osteoblasts and osteoclasts of SINFH mice were measured by tartaric acid phosphatase staining, CCK-8 and Annexin V-FITC/PI dual staining, respectively. GINS2 was up-regulated while p53/GADD45A was down-regulated in femoral tissues in SINFH patients and mice. GINS2 was down-regulated while p53 and GADD45A were up-regulated in osteoblasts of SINFH mice, which presented opposite trends in osteoclasts formations. GINS2 decreased osteoblasts activities and increased osteoclasts activities, inhibited apoptosis and promoted proliferation in osteoblasts and osteoclasts via p53/GADD45A pathway. GINS2 affects activity/differentiation, apoptosis and proliferation of osteoblast and osteoclast in steroid-induced osteonecrosis of the femoral head by regulating p53/GADD45A signaling pathway.

Abstract This study investigated the hyperspectral reflectance response of time series generated during oven drying to changes in the moisture content of potato tubers. Seventeen preprocessing methods were used to eliminate the influence of spectral noise on the spectral characteristic curve. Algorithms such as CatBoost, LightGBM, and XGBoost are used to obtain the first 40 effective characteristic spectra of hyperspectral images, which reduces the redundancy of data and improves the prediction accuracy. The water content prediction model of potato tubers was established by using the selected characteristic bands. The results showed that the combined model based on Lasso and XGBoost algorithm had the strongest prediction ability. The best model is MF-Lasso-XGBoost, which has R2 value of 0.8908, Rmse of 0.0610, Mdae of 0.0389, and R2cv of 0.8448. This research can provide reference for the detection of potato moisture content and theoretical basis for the development of crop moisture detector.

RESUMO: A biossíntese é a única fonte de amido de batata que é uma importante matéria-prima para o processamento de alimentos, amido modificado e energia de biomassa. No entanto, não está claro sobre a evolução da síntese do amido com o desenvolvimento do tubérculo na batata. O presente estudo teve como objetivo avaliar as diferenças nas propriedades de síntese e gelatinização do amido de tubérculos de batata com diferentes teores de amido. Em relação às cultivares de médio e baixo teor de amido, as cultivares de alto teor de amido apresentaram expressão do gene SBEII, atividade enzimática AGPase e SSS e teor de amido total significativamente maiores após o estágio intermediário de acúmulo de amido, bem como menor tamanho médio dos grânulos de amido durante todo o processo de desenvolvimento do tubérculo, maior temperatura de colagem antes dos estágios finais de crescimento do tubérculo e menor temperatura de colagem após o estágio intermediário de acúmulo de amido. A análise de trilha mostrou que, após o estágio intermediário de acúmulo de amido, os efeitos na gelatinização do amido de cultivares com alto, médio e baixo teor de amido representaram a atividade da enzima de síntese de amido> acúmulo de amido> distribuição de grânulos de amido> expressão gênica de enzima de síntese de amido; expressão gênica de enzima de síntese de amido > atividade da enzima de síntese de amido> acúmulo de amido> distribuição de grânulos de amido; expressão gênica da enzima de síntese de amido> distribuição de grânulos de amido> atividade de síntese de amido> acúmulo de amido, respectivamente. No estudo, as fases existentes na biossíntese do amido do tubérculo de batata, e a qualidade do amido e seu processo de formação foram diferentes entre as variedades com diferentes teores de amido. As descobertas podem contribuir para a aplicação de amido e as indústrias de batata.

ABSTRACT: Biosynthesis is the only source of potato starch which is an important raw material for food processing, modified starch and biomass energy. However, it is not clear about the evolution of starch synthesis with tuber development in potato. The present study evaluated the differences of starch synthesis and gelatinization properties of potato tubers with different starch content. Relative to cultivars of medium and low starch content, cultivars of high starch content showed significantly higher SBEII gene expression, AGPase and SSS enzyme activity, and total starch content after middle stage of starch accumulation, and had smaller average starch granule size during whole process of tuber development, and had higher pasting temperature before late stages of tuber growth, and had lower pasting temperature after middle stage of starch accumulation. Path analysis showed that, after middle stage of starch accumulation, effects on starch gelatinization of cultivars with high, medium and low starch content represented starch synthesis enzyme activity > starch accumulation > starch granule distribution > starch synthesis enzyme gene expression, starch synthesis enzyme gene expression > starch synthesis enzyme activity > starch accumulation > starch granule distribution, starch synthesis enzyme gene expression > starch granule distribution > starch synthesis enzyme activity > starch accumulation, respectively. In the study, phases existed in the starch biosynthesis of potato tuber, and the starch quality and its formation process were different among varieties with different starch content. The findings might contribute to starch application and potato industries.

Abstract In this study, the physicochemical properties of walnut (Juglans regia L.) oils collected from different sites in Khyber PakhtoonKhwa (KPK), Pakistan were investigated. The obtained walnut oils showed variation in viscosity, surface tension, density, acid, saponification, and iodine values. The oil obtained from the Madyan (Sample E) showed the lowest density (0.981 g/cm3), surface tension (34.76×10-3 N/m), viscosity (15.0595 cp) saponification values 84.15 (mg KOH/g of oil) and acid value 3.5 (mg KOH per gram oil) amongst all. The physicochemical properties of all the oils were also compared to olive oil (standard) where sample E showed similarities in such properties compared to olive oil. Moreover, higher concentrations of oleic acid (41 %) and linoleic acid (9 %) were detected in the walnut oil of sample E in GC/MS analysis. The similarities of walnut oil obtained from Madyan to that of standard (olive oil) thus affirm it as best quality oil and suitable for food processing purposes.

Resumen En este estudio se investigaron las propiedades fisicoquímicas del aceite de nuez (Juglans regia L.) recolectado de diferentes sitios en Khyber PakhtoonKhwa (KPK), Pakistán. Los aceites de nuez obtenidos mostraron variación en los valores de viscosidad, tensión superficial, densidad, acidez, saponificación e índice de yodo. El aceite obtenido del Madyan (Muestra E) mostró la menor densidad (0.981 g/cm3), tensión superficial (34.76 × 10-3 N/m), viscosidad (15.0595 cp), valores de saponificación 84.15 (mg KOH/g de aceite) y valor ácido 3.5 (mg KOH por gramo de aceite) entre otros. Las propiedades fisicoquímicas de todos los aceites también se compararon con el aceite de oliva (estándar); la muestra E mostró similitudes en las diferentes propiedades en comparación con el aceite de oliva. Además, se determinaron concentraciones más elevadas de ácido oleico (41 %) y ácido linoleico (9 %) en el aceite de nuez de la muestra E mediante el análisis GC/MS. Las similitudes del aceite de nuez obtenido de Madyan con el estándar (aceite de oliva) lo afirman como aceite de mejor calidad y adecuado para el procesamiento de alimentos.

Abstract To evaluate the effect of medical nutritional therapy (MNT) combined with low glycaemic index (LGI) formula at breakfast for 4 weeks on the glycaemic control of patients with type 2 diabetes mellitus (T2DM). Ninety-six participants were recruited and randomly equally divided into two groups. The intervention group was offered with 40g LGI formula at breakfast adjusted according to MNT, and the control group with regular MNT. After 4 weeks, fasting blood glucose, glycated albumin (GA), insulin, C-peptide levels and metabolic index were compared between the two groups. Compared to the intervention group, the total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and the change of FBG (–0.64 ± 1.4 vs. 0.3 ± 2.2; p < 0.05) and 2h-PBG (–1.8 ± 3.6 vs.0.8 ± 3.2; p < 0.01) in the control group were significantly decreased. The LGI formulations, having a significant effect on controlling glucose and lipid metabolism, could be used as a non-pharmacological intervention for T2DM self-management.

The goal of this study was to identify potential transcriptomic markers in pediatric septic shock prognosis by an integrative analysis of multiple public microarray datasets. Using the R software and bioconductor packages, we performed a statistical analysis to identify differentially expressed (DE) genes in pediatric septic shock non-survivors, and further performed functional interpretation (enrichment analysis and co-expression network construction) and classification quality evaluation of the DE genes identified. Four microarray datasets (3 training datasets and 1 testing dataset, 252 pediatric patients with septic shock in total) were collected for the integrative analysis. A total of 32 DE genes (18 upregulated genes; 14 downregulated genes) were identified in pediatric septic shock non-survivors. Enrichment analysis revealed that those DE genes were strongly associated with acute inflammatory response to antigenic stimulus, response to yeast, and defense response to bacterium. A support vector machine classifier (non-survivors vs survivors) was also trained based on DE genes. In conclusion, the DE genes identified in this study are suggested as candidate transcriptomic markers for pediatric septic shock prognosis and provide novel insights into the progression of pediatric septic shock.

Abstract: The great potential of crowdsourcing contest is bringing the issue of how to sustain solvers’ participation intention to be a hot topic in research and practice. This study uses the framework of Expectation-confirmation model to explain solvers’ continuance intention. Due to the uncertainties inherent in crowdsourcing contest, trust, a salient psychological belief, should be taken into account with the Expectation-confirmation model framework to predict solvers’ continuance intention. In addition, the intensive demand of intelligence and competition indicate interaction and fairness as two crucial factors for solvers to achieve expectation, thus suggesting that they may have influence on the confirmation level. Corresponding to these challenges, this study integrates platform trust, interaction, and perceived fairness into an extended Expectation-confirmation model to examine solvers’ continuance intention. Using a sample of 306 solvers, empirical results show that satisfaction, perceived benefits, and platform trust, which are positively associated with confirmation, are three significant antecedents of solvers’ continuance intention. Confirmation is further found to be significantly determined by interaction and perceived fairness. These findings provide some implications in both theory and practice for understanding the process of triggering sustained intention with an Expectation-confirmation model framework in crowdsourcing contest.