ABSTRACT The collembolan family Entomobryidae, one of the most numerous, diverse and widely distributed, was recently reviewed in a work that integrated molecular and morphological characters. The new classification includes seven subfamilies, one of which, Nothobryinae, is characterized by the presence of PAO, falcate mucro and a few chaetae on the trochanteral organ. This subfamily is composed of only three genera: Capbrya Barra, 1999 from South Africa, Hispanobrya Jordana & Baquero, 2005 from Spain and Nothobrya Arlé, 1961 from Brazil. Nothobrya (type species N. schubarti , described from the state of Pernambuco) remained monospecific for about half a century, when samples taken from urban areas of Rio de Janeiro city revealed its first record for the Brazilian Southeast. This new species, Nothobrya arlei sp. nov., is described and illustrated based on differences related to trochanteral organ, unguis, tenent hair shape and chateae on tenaculum. In this paper, characteristics of the macrochaetotaxy, sensillar and microsensillar sets and ventral tube chaetotaxy are provided, as well as a table with comparisons of Nothobrya species.