SUMMARY OBJECTIVE: The aim of the study was to compare the postoperative effects of endotracheal tube cuff inflation with alkalized lidocaine in patients undergoing thyroidectomy surgery. METHODS: This is a randomized, double-blind clinical trial between August 2020 and August 2022 at the Hospital São Domingos, São Luís, Maranhão, Brazil. Patients over 18 years who underwent thyroidectomy of both sexes, American Society of Anesthesiologists (ASA) I or ASA II, were included. Patients with difficult orotracheal intubation, smoking, cuff rupture during orotracheal intubation, heart, lung, or neuropathies, previous surgery of the larynx or trachea, with risk of aspiration of gastric contents, or with the need to use a nasogastric tube were excluded. Patients were randomly selected and divided into the control group, whose cuff was filled with 0.9% saline solution, and the alkalinized lidocaine group, where the cuff was filled with 2% lidocaine and 8.4% sodium bicarbonate. RESULTS: The control group had higher systolic blood pressure [137 (94–183) mmHg] medians after extubation than the alkalinized lidocaine group [127 (82–189) mmHg] (p=0.03). The same was observed in heart rate values [control group: 86 (62–120); alkalinized lidocaine group: 80 (53–120)] (p=0.034). The alkalinized lidocaine group showed a significant increase in the ability to sustain phonation in the 24 h postoperatively, from 82.0 to 98.0% (p=0.031). CONCLUSION: There was no protective effect of the use of alkalinized lidocaine on the sensation of swallowing and complaints after thyroidectomy surgery. There was a significant improvement in hemodynamic response in the intervention group after extubation. OBJECTIVE METHODS randomized doubleblind double blind 202 Domingos Luís Maranhão Brazil 1 sexes (ASA II included intubation smoking lung neuropathies trachea contents excluded 09 0 9 0.9 solution 2 84 8 4 8.4 bicarbonate RESULTS 137 [13 94–183 94183 94 183 (94–183 mmHg 127 [12 82–189 82189 82 189 (82–189 p=0.03. p003 p p=0.03 . 03 (p=0.03) 62–120 62120 62 120 (62–120) 53–120 53120 53 (53–120) p=0.034. p0034 p=0.034 034 (p=0.034) postoperatively 820 82. 980 98 98.0 p=0.031. p0031 p=0.031 031 (p=0.031) CONCLUSION 20 0. 8. 13 [1 94–18 9418 (94–18 12 82–18 8218 (82–18 p00 p=0.0 (p=0.03 62–12 6212 6 (62–120 53–12 5312 5 (53–120 (p=0.034 98. (p=0.031 [ 94–1 941 (94–1 82–1 821 (82–1 p0 p=0. (p=0.0 62–1 621 (62–12 53–1 531 (53–12 94– (94– 82– (82– p=0 (p=0. 62– (62–1 53– (53–1 (94 (82 p= (p=0 (62– (53– (9 (8 (p= (62 (53 ( (p (6 (5

Electrospinning, an economical technique, is widely used for biomedical scaffold fabrication, crucial in tissue and organ regeneration, particularly with biomaterials. Polymers, either pure or reinforced with ceramics, aid in cell proliferation and tissue formation. Polyhydroxybutyrate (PHB) is a promising biopolymer for tissue engineering, offering biocompatibility comparable to petroleum-derived polymers. Combining PHB with hydroxyapatite (HAp) enhances mechanical strength and osteoconductivity. This study aims to produce electrospun PHB microfibrous webs reinforced with HAp for scaffold fabrication. Morphological variations are analyzed through manipulation of electrospinning parameters. The study observed microfibrous webs with diameters ranging from 2 to 9 µm. Mechanical and microstructural evaluations demonstrate superior strength of PHB/HAp microfibrous webs compared to pure PHB, 1.23 MPa and 0.58 MPa respectively, demonstrating the efficacy of HAp reinforcement. These findings highlight the potential of PHB/HAp microfibrous webs in bone tissue engineering. Electrospinning technique fabrication regeneration biomaterials Polymers ceramics formation (PHB engineering petroleumderived petroleum derived polymers (HAp osteoconductivity parameters µm PHBHAp 123 1 23 1.2 058 0 58 0.5 respectively reinforcement 12 1. 05 5 0.

RESUMO Espécies do gênero Corymbia são consideradas potenciais alternativas ao eucalipto. Além de possuir madeira de qualidade superior, Corymbia spp. são tolerantes à maioria das pragas, doenças e estresses abióticos que afetam as plantações de eucalipto, incluindo distúrbios fisiológicos, déficit hídrico e danos causados pelo vento. No entanto, as tensões ambientais estimulam a produção de kino, o que diminui a qualidade da celulose e da madeira serrada. Este estudo teve como objetivo estabelecer um método para avaliação de kino e estimativa de parâmetros genéticos em Corymbia. Para isso foram utilizados 16 clones híbridos de Corymbia (C. citriodora × C. torelliana) e 5 clones de Eucalyptus. Dois métodos (M1 e M2) foram testados para avaliação; M1 consistiu na perfuração da casca com Pilodyn e M2 consistiu na perfuração do cerne com Pilodyn. Foram avaliados os seguintes parâmetros: incidência de exsudação, comprimento do exsudato que fluiu sobre o caule e peso do exsudato. Os parâmetros genéticos foram estimados por um método de modelo misto (REML/BLUP). A significância dos efeitos aleatórios do modelo estatístico foi testada pelo teste da razão de verossimilhança. Efeitos clones significativos foram obtidos para todos os parâmetros, exceto para o comprimento do exsudato avaliado por M2. Os parâmetros determinados por M1 apresentaram maior herdabilidade e precisão. Portanto, M1 deve ser preferido para avaliação de kino em Corymbia. eucalipto superior spp pragas fisiológicos vento entanto serrada 1 C (C torelliana Eucalyptus M (M exsudação REML/BLUP. REMLBLUP REML/BLUP . REML BLUP (REML/BLUP) verossimilhança precisão Portanto (REML/BLUP

ABSTRACT Species within the genus Corymbia are regarded as potential alternatives to Eucalyptus. In addition to having superior wood quality, Corymbia spp. are tolerant to most pests, diseases, and abiotic stresses that affecting Eucalyptus plantations, including physiological disorders, water deficit, and wind damage. However, environmental stresses stimulate kino production, which decreases the quality of pulp and sawn wood. This study aimed to develop a method for evaluating kinoand estimate genetic parameters in Corymbia. For this, 16 Corymbia (C. citriodora × C. torelliana) hybrid clones and 5 clones of Eucalyptus were used. Two evaluation methods (M1 and M2) were tested for kino evaluation; M1 consisted of drilling the bark with Pilodyn and M2 consisted of drilling the heartwood with Pilodyn. The following kino parameters were evaluated: exudation incidence, exudate length which flowed over the stem, and exudate weight. Genetic parameters were estimated by a mixed model method (REML/BLUP). The significance of random effects of the statistical model was tested by the likelihood ratio test. Significant clone effects were obtained for all kino parameters, except for exudate length as assessed by M2. Kino parameters determined by M1 exhibited higher heritability and accuracy. Therefore, M1 should be preferred for kino evaluation in Corymbia. spp pests diseases plantations disorders deficit damage However production this 1 C (C torelliana used M (M evaluated incidence stem weight REML/BLUP. REMLBLUP REML/BLUP . REML BLUP (REML/BLUP) test accuracy Therefore (REML/BLUP

SUMMARY OBJECTIVE: Therapy and vocal rehabilitation in laryngeal cancer impact patients’ quality of life. The objective of this study was to evaluate the evolution of the quality of life of patients with laryngeal cancer submitted to total laryngectomy and using electrolarynx. METHODS: This is an observational study with a cross-sectional design and a quantitative approach. It was conducted between April 2022 and January 2023 in a Brazilian cancer hospital. For data collection, a quality of life questionnaire, validated for patients with head and neck cancer at the University of Washington, was applied in two phases: from 7 days after total laryngectomy and, subsequently, from 70 days after surgery using electronic larynx for at least 60 days. The inclusion criteria were patients undergoing total laryngectomy included on the Aldenora Bello Cancer Hospital's election list to receive the electronic larynx. Patients who did not sign the informed consent form were not included. RESULTS: The sample consisted of 31 patients, of which approximately 84% were men and approximately 93% at the age of 50 years or older. When comparing the phases, it is possible to observe that the item speech had the greatest progress, while chewing had the least. Only the item recreation, swallowing, taste, and saliva did not show any statistical significance. The score for the general quality of life questions increased. CONCLUSION: Electronic larynx is a viable and useful method of voice rehabilitation. Our data suggest that the use of the electrolarynx as a postlaryngectomy method of verbal communication is responsible for positive effects on patients’ quality of life. OBJECTIVE METHODS crosssectional cross sectional approach 202 hospital collection questionnaire Washington phases subsequently 6 Hospitals Hospital s RESULTS 3 84 93 5 older progress recreation swallowing taste significance increased CONCLUSION 20 8 9 2

RESUMO O critério expandido para doadores foi estabelecido para reduzir o descarte de órgãos. Apesar disso, vários fatores podem levar à não utilização de rins inicialmente viáveis, como anormalidades anatômicas, lesões traumáticas e lesões iatrogênicas durante a cirurgia de captação de órgãos. Relatamos um caso de transplante renal em que foi identificada e reparada lesão capsular durante o preparo do órgão, bem como realizamos revisão da literatura sobre lesões renais em transplantes. Um paciente de 52 anos, diabético, hipertenso, com doença renal crônica, em tratamento dialítico há 2 anos, foi internado para transplante renal de doador falecido. O doador era um homem de 35 anos com morte encefálica secundária a traumatismo cranioencefálico em acidente de carro. Durante o preparo do órgão, foi identificada uma lesão capsular no polo superior do rim direito. Sutura contínua da cápsula renal foi realizada com fio CatGut 3-0 para corrigir o defeito e reconstruir a superfície renal. Após reperfusão renal, não foi observado sangramento ativo na área suturada. O paciente teve boa evolução pós-operatória, sem complicações. As principais complicações associadas às lesões capsulares e ao desnudamento capsular completo são o sangramento e a formação de hematomas, bem como o extravasamento urinário e linfático através do parênquima renal exposto. O tratamento pode envolver cauterização direta com eletrocautério ou plasma de argônio, ou uso de agentes hemostáticos. O reparo bem-sucedido de lesões capsulares previne complicações como sangramento durante a reperfusão e fístulas urinárias, e consiste em estratégia crucial para estimular o uso de todo o contingente de órgãos disponível, abrangendo maior número de receptores. disso viáveis anatômicas órgão transplantes 5 diabético hipertenso crônica falecido 3 carro direito 30 0 3- suturada pósoperatória, pósoperatória pós operatória, operatória pós-operatória hematomas exposto argônio hemostáticos bemsucedido sucedido urinárias disponível receptores

ABSTRACT Expanded donor criteria have been established to reduce organ wastage, as various factors can lead the disposal of kidneys, such as anatomical abnormalities, traumatic injuries, and iatrogenic injuries during organ retrieval surgery. We report a case of kidney transplantation in which a capsular lesion was identified and repaired during organ preparation (back table) and review of the literature regarding transplant kidney injuries. A diabetic and hypertensive 52-year-old patient with chronic kidney disease undergoing dialysis therapy for 2 years was admitted for a deceased donor kidney transplant. The donor was a 35-year-old man with brain death secondary to traumatic brain injury in a car accident. During the kidney preparation on the back table, a capsular lesion was visualized on the upper pole of the right kidney. Continuous suture of the renal capsule was performed using a 3-0 CatGut suture to correct the defect and reconstruct the renal surface. After renal reperfusion, no active bleeding was observed from the sutured area. The patient had a good postoperative course with no complications. The main complications associated with capsular lesions and complete capsular denudation are bleeding and hematoma formation, as well as urinary and lymphatic extravasation through the exposed renal parenchyma. Treatment may involve direct cauterization using electrocautery or argon plasma, or the use of hemostatic agents. Successful repair of capsular lesions not only prevents complications such as bleeding during reperfusion and urinary fistulae but also plays a crucial role in expanding the pool of organs available for transplantation. wastage kidneys abnormalities surgery table 52yearold yearold 52 year old 35yearold 35 accident 30 3 0 3- surface area formation parenchyma plasma agents 5

Abstract In the pediatric population, complex regional pain syndrome (CRPS) is a challenging condition that leads to chronic psychosocial dysfunction. This case is of a 12-year-old male patient, 50 kg, who, after twisting his right ankle, started to present intense pain. Without adequate diagnosis and treatment, his family sought a pain specialist. During the evaluation, a change in sensitivity and temperature was observed, besides hyperalgesia, allodynia, redness, edema, and motor dysfunction, being diagnosed with CRPS. Ultrasound-guided sciatic blocks were performed on the affected limb, increasing the interval between crises, reducing pain intensity, and promoting a return to daily activities. population CRPS (CRPS dysfunction 12yearold yearold 12 year old patient 5 kg who ankle treatment specialist evaluation observed hyperalgesia allodynia redness edema Ultrasoundguided Ultrasound guided limb crises intensity activities 1

Abstract Introduced in the Latin American scenario by the Colombian Paola Andrea Acosta Alvarado, Multilevel Constitutionalism had to adapt to a different reality from that found in Pernice's Europe. It so happens that the American continent does not have a supranational body similar to the European Union, satisfying for the purposes desired by Acosta Alvarado the Inter-American Human Rights System. In this context, knowing that one of the tools of Inter-American Multilevel Constitutionalism is interjurisdictional dialogue, this research will assess whether the dialogues that take place between the Federal Supreme Court and the Inter-American Court of Human Rights regarding pre-trial detention are fruitful.

Resumen Introducido en el escenario latinoamericano por la colombiana Paola Andrea Acosta Alvarado, el Constitucionalismo Multinivel tuvo que adaptarse a una realidad distinta a la de la Europa de Pernice. Sucede que el continente americano no cuenta con un organismo supranacional similar a lo de la Unión Europea, satisfaciendo para los fines deseados por Acosta Alvarado el Sistema Interamericano de Protección de los Derechos Humanos. En ese contexto, sabiendo que una de las herramientas del Constitucionalismo Multinivel Interamericano es el diálogo interjurisdiccional, esta investigación buscará evaluar si los diálogos que se dan entre el Supremo Tribunal Federal y la Corte Interamericana de Derechos Humanos sobre la prisión preventiva son provechosos.

RESUMO JUSTIFICATIVA E OBJETIVOS: A dor é uma das causas mais prevalentes de incapacidade no mundo, e os efeitos adversos promovidos pelos analgésicos podem limitar o sucesso terapêutico. Nesse contexto, surge o laser como terapia complementar que pode potencializar a analgesia, sem aumentar incidência de eventos adversos indesejáveis. O objetivo deste estudo foi realizar uma revisão sistemática sobre a eficácia e a eficiência do laser de alta intensidade (LAI) no tratamento da dor. CONTEÚDO: Foi realizada uma busca sistemática nas plataformas Medline, LILACS, Pubmed e PEDro, de julho de 2020 a agosto de 2022. As palavras chaves dor, dor crônica, laser de alta intensidade e tratamento foram consideradas. A qualidade dos estudos clínicos selecionados foi avaliada utilizando a escala PEDro. As revisões sistemáticas incluídas foram avaliadas quanto à qualidade metodológica através da ferramenta AMSTAR. A principal medida estudada foi a intensidade de dor. Foram encontrados 227 estudos e com base nos critérios de inclusão e exclusão, 32 artigos foram lidos na íntegra, tendo sido excluído um por não avaliar a dor. As desordens musculoesqueléticas corresponderam a 70,96% das doenças avaliadas e a escala analógica visual (EAV) foi a única ferramenta de mensuração da dor utilizada em 100% dos estudos. Aproximadamente 57% dos estudos tinham alta qualidade metodológica (PEDro=7). Em 53,84% dos ensaios o LAI foi utilizado como intervenção única, e em 46,16% foi associado a exercícios. Em 96,15% dos ensaios clínicos e 100% das revisões sistemáticas, o LAI promoveu alívio da dor. CONCLUSÃO: O LAI é uma modalidade eficaz para analgesia ao promover significativo alívio da dor, rápida recuperação e melhora na qualidade de vida dos pacientes de forma segura. A diversidade nos parâmetros de irradiação (dose, duração, intervalo e número de sessões) empregados, indica a necessidade de mais estudos randomizados para estabelecer sua eficiência em longo prazo. OBJETIVOS mundo terapêutico contexto indesejáveis (LAI CONTEÚDO Medline LILACS PEDro 202 2022 crônica consideradas AMSTAR 22 exclusão 3 íntegra 7096 70 96 70,96 EAV (EAV 100 57 PEDro=7. PEDro7 PEDro=7 . 7 (PEDro=7) 5384 53 84 53,84 4616 46 16 46,16 exercícios 9615 15 96,15 CONCLUSÃO segura dose, dose (dose duração sessões empregados prazo 20 2 709 9 70,9 10 5 PEDro= (PEDro=7 538 8 53,8 461 4 1 46,1 961 96,1 70, (PEDro= 53, 46, 96, (PEDro

ABSTRACT BACKGROUND AND OBJECTIVES: Pain is one of the most prevalent causes of disability in the world, and the adverse effects promoted by analgesics can limit therapeutic success. In this context, laser appears as a complementary therapy that can enhance analgesia without increasing the incidence of undesirable adverse events. The aim of this study was to carry out a systematic review on the effectiveness and efficiency of high intensity laser (HIL) in the treatment of pain. CONTENTS: A systematic search was carried out in Medline, LILACS, Pubmed and PEDro, from July 2020 to August 2022. The keywords pain, chronic pain, high intensity laser and treatment were considered. The quality of selected studies was assessed using the PEDro scale. Included systematic reviews were assessed for methodological quality using the AMSTAR tool. The main measure studied was pain intensity. 227 studies were found and, based on the inclusion and exclusion criteria, 32 articles were read in full, whit one being excluded for not assessing pain. Musculoskeletal disorders corresponded to 70,96% of the assessed diseases and the visual analogue scale (VAS) was the only pain measurement tool used in 100% of the studies. Approximately 57% of the studies were of high methodological quality (PEDro=7). In 53,84% of the trials, HIL was used as a single intervention, and in 46,16% it was associated whit exercises. In 96.15% of clinical trials and 100% of systematic reviews there were positive effects of HIL on pain. CONCLUSION: HIL is an effective modality for analgesia by promoting significant pain relief, rapid recovery and improvement in patient’s quality of life, in a safe way. The diversity in irradiation parameters (dose, duration, interval and number of sessions) used, indicates the need for further randomized studies to establish its long-term efficiency. OBJECTIVES world success context events (HIL CONTENTS Medline LILACS 202 2022 considered 22 criteria 3 full 7096 70 96 70,96 VAS (VAS 100 57 PEDro=7. PEDro7 PEDro=7 . 7 (PEDro=7) 5384 53 84 53,84 intervention 4616 46 16 46,16 exercises 9615 15 96.15 CONCLUSION relief patients patient s life way dose, dose (dose duration sessions longterm long term 20 2 709 9 70,9 10 5 PEDro= (PEDro=7 538 8 53,8 461 4 1 46,1 961 96.1 70, (PEDro= 53, 46, 96. (PEDro

SUMMARY OBJECTIVE: This study aimed to evaluate postoperative pain and quality of life in patients undergoing median sternotomy. METHODS: A cohort study was carried out on a sample of 30 patients who underwent elective cardiac surgery by longitudinal median sternotomy. Patients were interviewed at Intensive Care Unit discharge and hospital discharge, when the Visual Numeric Scale and the Brief Pain Inventory were applied, and 2 weeks after hospital discharge, when the World Health Organization Quality of Life-Bref questionnaire was administered. The normality of the results was analyzed by the Shapiro-Wilk test, and Wilcoxon Rank Sum and McNemar tests were utilized for the analysis of numerical and categorical variables. For correlation between numerical variables, Spearman's linear correlation test was applied. To compare numerical variables, Mann-Whitney U and Kruskal-Wallis tests were applied. Differences between groups were considered significant when the p-value was <0.05. RESULTS: Between Intensive Care Unit and hospital discharge, there was a reduction in median pain intensity assessed by the Visual Numeric Scale from 5.0 to 2.0 (p<0.001), as well as in eight Brief Pain Inventory parameters: worst pain intensity in the last 24 h (p=0.001), analgesic relief (p=0.035), and pain felt right now (p=0.009); and in interference in daily activities (p<0.001), mood (p=0.017), ability to walk (p<0.001), relationship with other people (p=0.005), and sleep (p=0.006). Higher pain intensity at Intensive Care Unit discharge was associated with worse performance in the psychological domain of quality of life at out-of-hospital follow-up. CONCLUSION: Proper management of post-sternotomy pain in the Intensive Care Unit may imply better quality of life at out-of-hospital follow-up. OBJECTIVE sternotomy METHODS 3 applied LifeBref Life Bref administered ShapiroWilk Shapiro Wilk variables Spearmans Spearman s MannWhitney Mann Whitney KruskalWallis Kruskal Wallis pvalue p value 005 0 05 <0.05 RESULTS 50 5 5. 20 2. p<0.001, p0001 p<0.001 , 001 (p<0.001) parameters p=0.001, p=0.001 (p=0.001) p=0.035, p0035 p=0.035 035 (p=0.035) p=0.009 p0009 009 (p=0.009) p=0.017, p0017 p=0.017 017 (p=0.017) p=0.005, p0005 p=0.005 (p=0.005) p=0.006. p0006 p=0.006 . 006 (p=0.006) outofhospital followup. followup follow up. up follow-up CONCLUSION poststernotomy post 00 <0.0 p000 p<0.00 (p<0.001 p=0.00 (p=0.001 p003 p=0.03 03 (p=0.035 (p=0.009 p001 p=0.01 01 (p=0.017 (p=0.005 (p=0.006 <0. p00 p<0.0 (p<0.00 p=0.0 (p=0.00 (p=0.03 (p=0.01 <0 p0 p<0. (p<0.0 p=0. (p=0.0 < p<0 (p<0. p=0 (p=0. p< (p<0 p= (p=0 (p< (p= (p

SUMMARY OBJECTIVE: This study aimed to comparatively analyze sociodemographic data and postoperative parameters of patients undergoing bypass and sleeve surgeries in a private hospital in São Luís, MA. METHODS: The study was descriptive, prospective, observational, and comparative, with a quantitative approach between August 2020 and July 2021. We analyzed 74 participants of both genders, aged between 18 and 70 years, with 31 undergoing Roux-en-Y gastric bypass surgery and 43 undergoing sleeve gastrectomy surgery. In the postoperative period, sociodemographic characteristics, surgery and anesthesia duration, pain levels, adverse effects, weight loss, and complications from the surgical procedure were analyzed. RESULTS: Males predominated in Roux-en-Y gastric bypass and females in sleeve gastrectomy surgery. Clinical characteristics regarding self-declared ethnicity, age and place of birth, education, and marital status were similar between the studied groups. Roux-en-Y gastric bypass had an average surgery time of 112.14±10.06 min and sleeve gastrectomy 91.11±23.69 min, with a significant difference (p<0.001). Regarding anesthesia time, gastric bypass averaged 160.36±13.99 min and sleeve gastrectomy 154.88±29.10 min, with no statistical difference between groups (p=0.335). Nausea, vomiting, and drowsiness were more common in Sleeve gastrectomy, with no significant difference (p=0.562). Roux-en-Y gastric bypass showed a higher rate of weight loss from 1 month after surgery (14.2±4.15) and more variation in body mass index within 3 months after surgery (32.17±4.76). Complications occurred in a small number of patients. CONCLUSION: The two surgical techniques proved effective in delivering the best results for patients, with the group undergoing bypass showing statistically significant weight loss from 1 month after the surgical procedure. OBJECTIVE Luís MA METHODS descriptive prospective observational comparative 202 2021 7 genders years RouxenY Roux en Y 4 period duration levels effects RESULTS selfdeclared self declared ethnicity birth education 112141006 112 14 10 06 112.14±10.0 91112369 91 11 23 69 91.11±23.6 p<0.001. p0001 p p<0.001 . 0 001 (p<0.001) 160361399 160 36 13 99 160.36±13.9 154882910 154 88 29 154.88±29.1 p=0.335. p0335 p=0.335 335 (p=0.335) Nausea vomiting p=0.562. p0562 p=0.562 562 (p=0.562) 14.2±4.15 142415 2 15 (14.2±4.15 32.17±4.76. 3217476 32.17±4.76 32 17 76 (32.17±4.76) CONCLUSION 20 11214100 112.14±10. 9111236 9 6 91.11±23. p000 p<0.00 00 (p<0.001 16036139 16 160.36±13. 15488291 8 154.88±29. p033 p=0.33 33 (p=0.335 p056 p=0.56 56 (p=0.562 14.2±4.1 14241 (14.2±4.1 321747 32.17±4.7 (32.17±4.76 1121410 112.14±10 911123 91.11±23 p00 p<0.0 (p<0.00 1603613 160.36±13 1548829 154.88±29 p03 p=0.3 (p=0.33 p05 p=0.5 5 (p=0.56 14.2±4. 1424 (14.2±4. 32174 32.17±4. (32.17±4.7 112141 112.14±1 91112 91.11±2 p0 p<0. (p<0.0 160361 160.36±1 154882 154.88±2 p=0. (p=0.3 (p=0.5 14.2±4 142 (14.2±4 3217 32.17±4 (32.17±4. 11214 112.14± 9111 91.11± p<0 (p<0. 16036 160.36± 15488 154.88± p=0 (p=0. 14.2± (14.2± 321 32.17± (32.17±4 1121 112.14 911 91.11 p< (p<0 1603 160.36 1548 154.88 p= (p=0 14.2 (14.2 32.17 (32.17± 112.1 91.1 (p< 160.3 154.8 (p= 14. (14. 32.1 (32.17 112. 91. (p 160. 154. (14 32. (32.1 (1 (32. ( (32 (3

BACKGROUND The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants can infect common mice inducing significant pathological lung lesions and inflammatory responses. This substantially mimics coronavirus disease 19 (COVID-19) infection and pathogenesis in humans. OBJECTIVES To characterise the effects of recombinant SARS-CoV-2 S1 receptor-binding domain (RBD) peptide in murine macrophage and microglial cells’ immune activation compared with classical PAMPs in vitro. METHODS Murine RAW 264.7 macrophages and BV2 microglial cells were exposed to increasing concentrations of the RBD peptide (0.01, 0.05, and 0.1 µg/mL), Lipopolysaccharide (LPS) and Poly(I:C) and evaluated after two and 24 h for significant markers of macrophage activation. We determined the effects of RBD peptide on cell viability, cleaved caspase 3 expressions, and nuclear morphometry analysis. FINDINGS In RAW cells, RBD peptide was cytotoxic, but not for BV2 cells. RAW cells presented increased arginase activity and IL-10 production; however, BV2 cells expressed iNOS and IL-6 after RBD peptide exposure. In addition, RAW cells increased cleaved-caspase-3, apoptosis, and mitotic catastrophe after RBD peptide stimulation but not BV2 cells. CONCLUSION RBD peptide exposure has different effects depending on the cell line, exposure time, and concentration. This study brings new evidence about the immunogenic profile of RBD in macrophage and microglial cells, advancing the understanding of SARS-Cov2 immuno- and neuropathology. SARSCoV2 SARSCoV SARS CoV (SARS-CoV-2 responses 1 COVID19 COVID (COVID-19 humans SARS-CoV- S receptorbinding receptor binding (RBD vitro 2647 264 7 264. BV 0.01, 001 0 01 (0.01 005 05 0.05 0. µg/mL, µgmL µg/mL , µg mL µg/mL) LPS (LPS PolyIC Poly I C Poly(I:C viability expressions analysis cytotoxic IL10 IL 10 IL-1 production however IL6 6 IL- addition cleavedcaspase3, cleavedcaspase3 cleavedcaspase 3, cleaved-caspase-3 apoptosis line time concentration SARSCov2 SARSCov Cov2 Cov SARS-Cov immuno neuropathology (SARS-CoV- COVID1 (COVID-1 SARS-CoV 26 0.01 00 (0.0 0.0 IL1 cleaved-caspase- (SARS-CoV (COVID- (0. cleaved-caspase (COVID (0 (

RESUMO O pé torto congênito (PTC) é uma das deformidades congênitas mais comuns dos membros inferiores, com incidência estimada de um para cada 1.000 nascidos vivos. Seu tratamento é motivo de controvérsia, mas, atualmente, o método de Ponseti tem sido difundido, mostrando resultados promissores e diminuindo a necessidade das liberações cirúrgicas extensas, como geralmente se fazia até a introdução da técnica. Utilizado em aproximadamente 55 países, o método apresenta alterações e alcances que variam de acordo com a comunidade em que é aplicado, sendo o resultado e as peculiaridades do método moldados de acordo com a amostra estudada. Objetivo: Avaliar o resultado clínico do tratamento do PTC através do método de Ponseti em condições locais. Métodos: A avaliação clínica incluiu uma análise descritiva da amostra, além de avaliação radiográfica e satisfação da família com o tratamento. Resultados: 46% dos pacientes apresentaram bons resultados e nenhuma família se mostrou insatisfeita. Não foram encontradas relações estatisticamente relevantes entre as variáveis estudadas. Conclusão: Os resultados são bons e, de maneira geral, semelhantes àqueles da literatura. Há, também, concordância geral dos dados epidemiológicos deste estudo com os relatados por outros autores. Nível de Evidência IV, Série de Casos. (PTC inferiores 1000 1 000 1.00 vivos controvérsia mas atualmente difundido extensas técnica 5 países aplicado estudada Objetivo locais Métodos Resultados 46 insatisfeita estudadas Conclusão literatura Há também autores IV Casos 100 00 1.0 4 10 0 1.

ABSTRACT Congenital clubfoot is one of the most common congenital deformities of the lower limbs, with an estimated incidence of 1 for every 1,000 live births. Its treatment is controversial, and currently the Ponseti method has been widespread, showing promising results and decreasing the need for extensive surgical releases, as was usually done until the introduction of the technique. Currently used in approximately 55 countries, the method has changes and scopes that vary according to the society in which it is applied, with the results and peculiarities of the method molded according to the sample studied. Objective: To evaluate the clinical outcome of clubfoot treatment using the Ponseti method under local conditions. Methods: The clinical evaluation will include a descriptive analysis of the sample, as well as radiographic evaluation and family satisfaction with the treatment. Results: In total, 46% of the patients had good results and no family was dissatisfied with the treatment results. No statistically relevant relationships were found between the studied variables. Conclusion: The results are good and generally similar to those in the literature. Our epidemiological data generally agree with those reported by other authors. Level of Evidence IV, Case Series. limbs 1000 000 1,00 births controversial widespread releases technique 5 countries applied Objective conditions Methods Results total 46 variables Conclusion literature authors IV Series 100 00 1,0 4 10 0 1,

SUMMARY OBJECTIVE: This study aimed to evaluate the quality of life of patients with endometriosis before and after surgical treatment. METHODS: An observational, longitudinal, and prospective study was conducted with 102 women with pelvic pain and endometriosis that was unimproved by clinical treatment and indicated for surgical treatment. The patients’ quality of life was assessed using the 30-item Endometriosis Health Profile (EHP-30) questionnaire before and 3 and 6 months after surgery. The statistical tests were analyzed using the Statistical Package for Social Sciences version 17.0, and the Friedman test was used. RESULTS: There was a reduction in EHP-30 scores 3 and 6 months after surgery compared to before surgery, as well as 6 months after surgery compared to 3 months after surgery, in the central questionnaire (PART 1) and in Sections A, B, C, E, and F (p<0.0001). For Section D, there was a reduction in scores 6 months after surgery compared to before surgery (p<0.0001). CONCLUSION: Surgical treatment of endometriosis improves quality of life in several areas assessed by the EHP-30 questionnaire. OBJECTIVE METHODS observational longitudinal 10 30item item 30 EHP30 EHP (EHP-30 170 17 0 17.0 used RESULTS EHP-3 PART 1 A B C E p<0.0001. p00001 p p<0.0001 . 0001 (p<0.0001) D CONCLUSION EHP3 (EHP-3 17. EHP- p0000 p<0.000 000 (p<0.0001 (EHP- p000 p<0.00 00 (p<0.000 (EHP p00 p<0.0 (p<0.00 p0 p<0. (p<0.0 p<0 (p<0. p< (p<0 (p< (p

Resumo Objetivo identificar a prevalência de multimorbidade em pessoas idosas residentes na zona rural e os fatores sociodemográficos, comportamentais e clínico-terapêuticos associados. Método estudo transversal, realizado com idosos residentes na zona rural de Araçagi, Paraíba, Brasil, vinculados à Estratégia Saúde da Família e selecionados aleatoriamente. A variável dependente do estudo foi a multimorbidade, entendida como a presença de duas ou mais condições crônicas em um só indivíduo. Os dados foram coletados através de um questionário sociodemográfico e um formulário sobre problemas de saúde autorreferidos, abordando 32 condições. Na análise dos dados, utilizou-se a estatística univariada, bivariada e regressão de Poisson. Resultados participaram do estudo 360 idosos com prevalência de multimorbidade de 54,2% (IC95%: 49,0–59,3). Após regressão, identificou-se que sexo feminino (RP=1,16; IC95%: 1,09–1,25), idade ≥ 70 anos (RP=1,08; IC95%: 1,01–1,15), sobrepeso (RP=1,19; IC95%: 1,10–1,29), acesso a água tratada e encanada (RP=1,09; IC95%: 1,00–1,18), histórico de tabagismo (RP=1,10; IC95%: 1,03–1,17), não fazer uso de álcool (RP=1,13; IC95%: 1,05–1,22), dor crônica (RP=1,18; IC95%: 1,10–1,26), hospital como primeira opção de serviço de saúde (RP=1,12; IC95%: 1,03–1,21) e consulta médica no último ano (RP=1,19; IC95%: 1,11–1,27) eram fatores associados à multimorbidade. Conclusão o estudo revelou alta prevalência de multimorbidade e seus fatores associados. Por fim, torna-se viável o planejamento de medidas que melhorem a condição de saúde desses indivíduos e pensar em possibilidades de promover um envelhecimento saudável. sociodemográficos clínicoterapêuticos clínico terapêuticos transversal Araçagi Paraíba Brasil aleatoriamente indivíduo autorreferidos 3 utilizouse utilizou se univariada Poisson 36 542 54 2 54,2 IC95% IC95 IC (IC95% 49,0–59,3. 490593 49,0–59,3 . 49 0 59 49,0–59,3) identificouse identificou RP=1,16 RP116 RP 1 16 (RP=1,16 1,09–1,25, 109125 1,09–1,25 , 09 25 1,09–1,25) 7 RP=1,08 RP108 08 (RP=1,08 1,01–1,15, 101115 1,01–1,15 01 15 1,01–1,15) RP=1,19 RP119 19 (RP=1,19 1,10–1,29, 110129 1,10–1,29 10 29 1,10–1,29) RP=1,09 RP109 (RP=1,09 1,00–1,18, 100118 1,00–1,18 00 18 1,00–1,18) RP=1,10 RP110 (RP=1,10 1,03–1,17, 103117 1,03–1,17 03 17 1,03–1,17) RP=1,13 RP113 13 (RP=1,13 1,05–1,22, 105122 1,05–1,22 05 22 1,05–1,22) RP=1,18 RP118 (RP=1,18 1,10–1,26, 110126 1,10–1,26 26 1,10–1,26) RP=1,12 RP112 12 (RP=1,12 1,03–1,21 103121 21 1,11–1,27 111127 11 27 fim tornase torna saudável 5 54, IC9 (IC95 49059 49,0–59, 4 RP=1,1 RP11 (RP=1,1 10912 1,09–1,2 RP=1,0 RP10 (RP=1,0 10111 1,01–1,1 11012 1,10–1,2 10011 1,00–1,1 10311 1,03–1,1 10512 1,05–1,2 1,03–1,2 10312 1,11–1,2 11112 (IC9 4905 49,0–59 RP=1, RP1 (RP=1, 1091 1,09–1, 1011 1,01–1, 1101 1,10–1, 1001 1,00–1, 1031 1,03–1, 1051 1,05–1, 1,11–1, 1111 (IC 490 49,0–5 RP=1 (RP=1 109 1,09–1 101 1,01–1 110 1,10–1 100 1,00–1 103 1,03–1 105 1,05–1 1,11–1 111 49,0– RP= (RP= 1,09– 1,01– 1,10– 1,00– 1,03– 1,05– 1,11– 49,0 (RP 1,09 1,01 1,10 1,00 1,03 1,05 1,11 49, 1,0 1,1 1,

Abstract Objective to identify the prevalence of multimorbidity in elderly people living in rural areas and associated sociodemographic, behavioral and clinical-therapeutic factors. Method cross-sectional study, conducted with randomly selected elderly residents in the rural area of Araçagi, Paraíba, Brazil, linked to the Family Health Strategy. The dependent variable of the study was multimorbidity, understood as the presence of two or more chronic conditions in a single individual. Data were collected through a sociodemographic questionnaire and a form about self-reported health problems, addressing 32 conditions. Univariate and bivariate statistics and Poisson regression were used in the data analysis, considering significant when p-value <0.05. Results 360 elderly subjects participated in the study, with a 54.2% (95%CI: 49,0–59,3) prevalence of multimorbidity. After regression, it was identified that female gender (PR=1,16; 95%CI: 1,09–1,25), age ≥70 years (PR=1,08; 95%CI: 1,01–1,15), overweight (PR=1,19; 95%CI: 1,10–1,29), access to treated and piped water (PR=1,09; 95%CI: 1,00–1,18), smoking history (PR=1,10; 95%CI: 1,03–1,17), not using alcohol (PR=1,13; 95%CI: 1,05–1,22), chronic pain (PR=1,18; 95%CI: 1,10–1,26), hospital as first choice of health service (PR=1,12; 95%CI: 1,03–1,21) and medical consultation in the last year (PR=1,19; 95%CI: 1,11–1,27) were factors associated with multimorbidity. Conclusion the study revealed a high prevalence of multimorbidity and its associated factors. Finally, it becomes feasible to plan measures that improve the health condition of these individuals and think of possibilities to promote healthy aging. clinicaltherapeutic clinical therapeutic crosssectional cross sectional Araçagi Paraíba Brazil Strategy individual selfreported self reported problems 3 analysis pvalue p value 005 0 05 <0.05 36 542 54 2 54.2 95%CI 95CI CI 95 (95%CI 49,0–59,3 490593 49 59 PR=1,16 PR116 PR 1 16 (PR=1,16 1,09–1,25, 109125 1,09–1,25 , 09 25 1,09–1,25) 70 ≥7 PR=1,08 PR108 08 (PR=1,08 1,01–1,15, 101115 1,01–1,15 01 15 1,01–1,15) PR=1,19 PR119 19 (PR=1,19 1,10–1,29, 110129 1,10–1,29 10 29 1,10–1,29) PR=1,09 PR109 (PR=1,09 1,00–1,18, 100118 1,00–1,18 00 18 1,00–1,18) PR=1,10 PR110 (PR=1,10 1,03–1,17, 103117 1,03–1,17 03 17 1,03–1,17) PR=1,13 PR113 13 (PR=1,13 1,05–1,22, 105122 1,05–1,22 22 1,05–1,22) PR=1,18 PR118 (PR=1,18 1,10–1,26, 110126 1,10–1,26 26 1,10–1,26) PR=1,12 PR112 12 (PR=1,12 1,03–1,21 103121 21 1,11–1,27 111127 11 27 Finally aging <0.0 5 54. 9 49,0–59, 49059 4 PR=1,1 PR11 (PR=1,1 10912 1,09–1,2 7 ≥ PR=1,0 PR10 (PR=1,0 10111 1,01–1,1 11012 1,10–1,2 10011 1,00–1,1 10311 1,03–1,1 10512 1,05–1,2 1,03–1,2 10312 1,11–1,2 11112 <0. 49,0–59 4905 PR=1, PR1 (PR=1, 1091 1,09–1, 1011 1,01–1, 1101 1,10–1, 1001 1,00–1, 1031 1,03–1, 1051 1,05–1, 1,11–1, 1111 <0 49,0–5 490 PR=1 (PR=1 109 1,09–1 101 1,01–1 110 1,10–1 100 1,00–1 103 1,03–1 105 1,05–1 1,11–1 111 < 49,0– PR= (PR= 1,09– 1,01– 1,10– 1,00– 1,03– 1,05– 1,11– 49,0 (PR 1,09 1,01 1,10 1,00 1,03 1,05 1,11 49, 1,0 1,1 1,