RESUMO: A enxertia cutânea é uma técnica cirúrgica simples e bastante útil para o reparo de feridas, principalmente, quando há dificuldade em realizar a síntese direta ou outras técnicas reconstrutivas. O laser de baixa intensidade (LBI) já foi utilizado com sucesso em casos em que o enxerto foi aplicado em feridas logo após a sua criação. No entanto, a LBI ainda carece de padronizações, mediante estudos conflitantes sobre seus resultados. Desta forma, este trabalho objetivou comparar a eficácia da LBI de onda contínua com onda pulsada na estimulação da cicatrização de enxertos cutâneos, aplicados em feridas recém-criadas em coelhos. Para tanto, foram utilizados 31 coelhos distribuídos em: grupo controle (GC, n = 10), grupo laser onda contínua (GLC; n = 10) e grupo laser onda pulsada (GLP; n = 11). O manejo pós operatório foi semelhante entre todos os grupos, com exceção da aplicação do laser, conforme o grupo indicado. Os grupos GLC e GLP apresentaram evolução semelhante, com processo cicatricial satisfatório do enxerto, ao contrário do grupo GC. Tais resultados se mantiveram na avaliação microscópica, pois os grupos tratados com laser apresentaram menor inflamação, melhor integração à área receptora, maior reepitelização e colagenização. Não houve diferença na densidade vascular entre os grupos de tratamento na análise imuno-histoquímica. A ferida recém-criada não é capaz de fornecer nutrição para o enxerto cutâneo nos coelhos, mas a LBI é uma terapia adjuvante eficiente em estimular a cicatrização entre a área receptora e o enxerto, promovendo integração total. Entretanto, embora não houve diferença estatística na forma de emissão de luz, pulsada ou contínua, os pacientes que receberam LBI em onda contínua apresentam evolução microscópica superior. RESUMO principalmente reconstrutivas (LBI criação entanto padronizações cutâneos recémcriadas recém criadas tanto 3 GC, GC (GC 10, 10 , (GLC (GLP 11. 11 . 11) indicado inflamação colagenização imunohistoquímica. imunohistoquímica imuno histoquímica. histoquímica imuno-histoquímica recémcriada criada total Entretanto luz superior 1

ABSTRACT: Skin grafting is a simple and very useful surgical technique for wound repair, especially where there is difficulty in performing direct synthesis or other reconstructive techniques. low-intensity laser (LIL) has already been used successfully in cases where the graft was applied to wounds shortly after its creation. However, LIL still lacks standardization due to conflicting studies on its results. Thus, this study compared the effectiveness of continuous wave LIL with pulsed wave LIL in stimulating the healing of skin grafts, applied to newly created wounds in rabbits. For this purpose, 31 rabbits were distributed into: control group (CG, n = 10), continuous wave laser group (GLC; n = 10) and pulsed wave laser group (GLP; n = 11). Postoperative management was similar between all groups, with the exception of laser application, as indicated by the group. The GLC and GLP groups showed similar evolution, with a satisfactory healing process of the graft, unlike the GC group. These results were maintained in the microscopic evaluation, as the groups treated with laser showed less inflammation, better integration with the receptor area, greater re-epithelialization and collagenization. There was no difference in vascular density between the treatment groups on immunohistochemical analysis. The newly created wound was not able to provide nutrition for the skin graft in rabbits, but LIL is an efficient adjuvant therapy in stimulating healing between the recipient area and the graft, promoting full integration. However; although, statistically there was no difference in the form of light emission, pulsed or continuous, patients who received continuous wave LIL showed superior microscopic evolution. ABSTRACT repair techniques lowintensity low intensity (LIL creation However Thus grafts purpose 3 into CG, CG (CG 10, 10 , (GLC (GLP 11. 11 . 11) application evolution evaluation inflammation reepithelialization re epithelialization collagenization analysis although emission 1

Resumo Defeitos de grandes segmentos traqueais podem apresentar sério problema clínico, devido à ausência de substitutos na cirurgia reconstrutiva. Os biomateriais poliméricos estão entre os mais utilizados na medicina. Entretanto, a implantação de um biomaterial desencadeia uma série de eventos biológicos e a biocompatibilidade do material é de extrema importância para a medicina regenerativa. Objetivou-se com este estudo avaliar o uso da copoliamida associada ao elastômero termoplástico (PCTPE) em próteses impressas em 3D para reparação de defeitos parciais da traqueia de coelhos. Foram utilizados 16 coelhos machos da raça Nova Zelândia Branco, submetidos à criação de um defeito parcial de traqueia na região ventral do 4º ao 8º anel traqueal. Os animais foram subdivididos (n=4) de acordo com o momento das eutanásias, programadas com intervalo de sete (G7), quinze (G15), trinta (G30) e sessenta dias (G60). A análise histopatológica por hematoxilina e eosina (HE) revelou que o implante 3D de PCTPE apresentou reação do tipo corpo estranho e a inflamação persistiu até os 60 dias de avaliação. A histoquímica de picrosirius vermelho revelou tendência de maior quantidade de colágeno tipo I nos tempos iniciais de inflamação, enquanto o colágeno tipo III foi predominante nos períodos tardios de avaliação. Pode-se sugerir reação exacerbada e não adequada da interface tecido-material e perpetuação do processo inflamatório. clínico reconstrutiva Entretanto regenerativa Objetivouse Objetivou se (PCTPE D 1 Branco º traqueal n=4 n4 n 4 (n=4 eutanásias G7, G7 G , (G7) G15, G15 (G15) G30 (G30 G60. G60 . (G60) HE (HE 6 avaliação Podese Pode tecidomaterial tecido inflamatório n= (n= (G7 G1 (G15 G3 (G3 G6 (G60 (n (G (G1 (G6

Abstract Large segmental tracheal defects can pose a serious clinical challenge owing to the lack of suitable substitutes for reconstructive surgery. Polymeric biomaterials are widely used in medicine. However, the implantation of biomaterials triggers a series of biological events, and material biocompatibility is of paramount importance in regenerative medicine. The objective of this study was to evaluate the use of a copolyamide associated with thermoplastic elastomer (PCTPE) in 3D-printed prostheses for repairing partial tracheal defects in rabbits. Sixteen male New Zealand White rabbits were used, and partial tracheal defects were created in the ventral region from the 4th to the 8th tracheal ring. The animals were subdivided into groups (n=4) based on the time of euthanasia, scheduled at seven days (G7), 15 days (G15), 30 days (G30), and 60 days (G60). Histopathological analysis with hematoxylin and eosin staining revealed that the 3D implant of PCTPE exhibited a foreign body reaction, and inflammation persisted for up to 60 days. Histochemistry with picrosirius red revealed a tendency for a greater amount of type I collagen to accumulate in the early stages of inflammation, whereas type III collagen was predominant in later evaluation periods. These findings suggest an exacerbated and inadequate tissue-material interface reaction and perpetuation of the inflammatory process. surgery medicine However events (PCTPE 3Dprinted Dprinted printed D th ring n=4 n4 n 4 (n=4 euthanasia G7, G7 G , (G7) 1 G15, G15 (G15) 3 G30, G30 (G30) 6 G60. G60 . (G60) periods tissuematerial tissue process n= (n= (G7 G1 (G15 G3 (G30 G6 (G60 (n (G (G1 (G3 (G6

ABSTRACT Purpose: The current study aimed at evaluating the repair of a partial defect of the trachea with a muscle flap, an advanced technique that employs combined suture patterns. Methods: Sixteen healthy male New Zealand white rabbits were used as an experimental model. A partial defect in the trachea within the ventral region of the fourth to eighth tracheal ring was created. Subsequently, repair was initiated with a flap of the sternocephalicus muscle. The animals were divided into four groups for postoperative evaluation using clinical, tracheoscopic, and histopathological analyses. Each group was separated according to the time of euthanasia, programmed at interval of seven (G7), 15 (G15), 30 (G30), and 60 days (G60). Results: One animal from the G60 group died, whereas the other animals had good surgical recovery without serious changes in the breathing pattern. The major clinical signs observed were stridor and coughing. Tracheoscopy revealed secretions in the tracheal lumen, exuberant granulation, and stenosis. Histopathological analysis showed growth of the ciliary respiratory epithelium at the flap site 30 days after implantation. Conclusions: Partial repair showed satisfactory results owing to the anatomical location of the muscle, adequate vascular support, and structural and physiological maintenance without serious changes in the respiratory system. Purpose patterns Methods model created Subsequently tracheoscopic analyses euthanasia G7, G7 G , (G7) 1 G15, G15 (G15) 3 G30, G30 (G30) 6 G60. . (G60) Results G6 died pattern coughing lumen granulation stenosis implantation Conclusions support system (G7 G1 (G15 G3 (G30 (G60 (G (G1 (G3 (G6

ABSTRACT Purpose: Laparoscopic cholecystectomy (LC) is the gold standard for the treatment of gallbladder (GB) disease in small animals. The aims of this study were to investigate and compare the effect of different types of dissectors during LC in rabbits; electrothermal bipolar vessel sealing device (EBVS-LigaSure) and standard electrosurgical dissection (bipolar Maryland) for dissection of the GB in LC, correlating liver function tests (LFTs) in pre and postoperative periods (days 0, 3, 7, 15); macroscopic checking 15 days after surgery through necropsy; histopathological, bacteriological through bacterial growth by culture and intraoperative complications. Methods: Twenty rabbits were used, group (n = 10) using EBVS for GB dissection and cystic duct seal (GLL), and group (n = 10) using bipolar dissecting forceps and EVBS for cystic duct seal (GLE). Results: A higher concentration of alkaline phosphatase was observed on GLL 15 days after surgery when compared to GLE. In addition, GLE resulted in a higher concentration of alanine aminotransferase at three days when compared to GLL. Conclusion: In LC no significant statistical differences were found between EBVS and bipolar Maryland; both devices are equally safe and effective in LC. Further studies are required to evaluate the effectiveness of these devices in animals with gallbladder pathologies. Therefore, clinical studies are necessary. Purpose (LC (GB EBVSLigaSure LigaSure (EBVS-LigaSure Maryland LFTs (LFTs 0 3 7 15) 1 necropsy histopathological complications Methods used n 10 GLL, , (GLL) . (GLE) Results addition Conclusion pathologies Therefore necessary (GLL (GLE

Resumo Os cães das raças braquicefálicas incluindo os Shih tzu, são predispostos ao desenvolvimento de afecções oftálmicas em razão da sua conformação facial. O objetivo principal do presente trabalho foi investigar as principais oftalmopatias em cães da raça Shih tzu atendidos no Hospital Veterinário “Governador Laudo Natel” da Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal-São Paulo, Brasil, entre os anos de 2014 e 2022. Foram selecionadas 574 fichas totalizando 1724 diagnósticos. Em relação sexo 50% (287/574) eram fêmeas e 50% (287/574) eram machos. Em relação à classificação anatômica os diagnósticos do grupo 1 (sistema lacrimal, pálpebras e anexos) foram os mais expressivos com 55% (945/1724), seguido do grupo 2 (segmento anterior) com 40% (683/1724), grupo 4 (bulbo ocular) com 4% (73/1724) e grupo 3 (segmento posterior) com 1% (23/1724). A idade média do diagnóstico foi de 5,2 anos, sendo que os cães adultos foram os mais representativos com 55% (317/574), seguido dos jovens 25% (145/574) e dos idosos com 20% (112/574). Os cães idosos obtiveram mais diagnósticos de glaucoma e de catarata quando comparados aos animais jovens e adultos. Em cães jovens as afecções mais diagnosticadas foram as ceratites ulcerativas e distiquíase, enquanto nos adultos e nos idosos foram ceratoconjuntivite seca e ceratite ulcerativa. facial Governador Natel Filho JaboticabalSão Jaboticabal São Paulo Brasil 201 2022 57 172 50 287/574 287574 287 (287/574 machos sistema lacrimal anexos 55 945/1724, 9451724 945/1724 , 945 (945/1724) segmento anterior 40 683/1724, 6831724 683/1724 683 (683/1724) bulbo ocular 73/1724 731724 73 (73/1724 posterior 23/1724. 231724 23/1724 . 23 (23/1724) 52 5 5, 317/574, 317574 317/574 317 (317/574) 25 145/574 145574 145 (145/574 20 112/574. 112574 112/574 112 (112/574) distiquíase ulcerativa 202 17 287/57 28757 28 (287/57 945172 945/172 94 (945/1724 683172 683/172 68 (683/1724 73/172 73172 7 (73/172 23172 23/172 (23/1724 31757 317/57 31 (317/574 145/57 14557 14 (145/57 11257 112/57 11 (112/574 287/5 2875 (287/5 94517 945/17 9 (945/172 68317 683/17 6 (683/172 73/17 7317 (73/17 2317 23/17 (23/172 3175 317/5 (317/57 145/5 1455 (145/5 1125 112/5 (112/57 287/ (287/ 9451 945/1 (945/17 6831 683/1 (683/17 73/1 731 (73/1 231 23/1 (23/17 317/ (317/5 145/ (145/ 112/ (112/5 (287 945/ (945/1 683/ (683/1 73/ (73/ 23/ (23/1 (317/ (145 (112/ (28 (945/ (683/ (73 (23/ (317 (14 (112 (2 (945 (683 (7 (23 (31 (1 (11 ( (94 (68 (3 (9 (6

Abstract Brachycephalic breeds, including Shih Tzus, are predisposed to developing ophthalmic conditions due to facial conformation. This study investigated the primary ophthalmic diseases in Shih Tzus treated at the Governador Laudo Natel’s Veterinary Hospital of São Paulo State University Júlio de Mesquita Filho, Jaboticabal-São Paulo, Brazil, between 2014 and 2022. We selected 574 cases, totaling 1,724 diagnoses. Regarding sex, 50% (287/574) were female, and 50% (287/574) were male. Anatomically, diagnoses in group 1 (lacrimal system, eyelids, and adnexa) were the most common, comprising 55% (945/1,724), followed by group 2 (anterior segment) at 40% (683/1,724), group 4 (eyeball) at 4% (73/1,724), and group 3 (posterior segment) at 1% (23/1,724). The mean age at diagnosis was 5.2 years. Adult dogs were the most represented age group at 55% (317/574), followed by young dogs at 25% (145/574) and elderly dogs at 20% (112/574). Elderly dogs had more diagnoses of glaucoma and cataracts compared to young and adult dogs. In young dogs, the most diagnosed conditions were ulcerative keratitis and distichiasis, while in adults and elderly dogs, the predominant conditions were keratoconjunctivitis sicca and ulcerative keratitis. breeds conformation Natels Natel s Filho JaboticabalSão Jaboticabal Brazil 201 2022 57 cases 1724 724 1,72 sex 50 287/574 287574 287 (287/574 female male Anatomically lacrimal system eyelids adnexa common 55 945/1,724, 9451724 945/1,724 , 945 (945/1,724) anterior segment 40 683/1,724, 6831724 683/1,724 683 (683/1,724) eyeball (eyeball 73/1,724, 731724 73/1,724 73 (73/1,724) posterior 23/1,724. 231724 23/1,724 . 23 (23/1,724) 52 5 5. years 317/574, 317574 317/574 317 (317/574) 25 145/574 145574 145 (145/574 20 112/574. 112574 112/574 112 (112/574) distichiasis 202 172 72 1,7 287/57 28757 28 (287/57 945172 945/1,72 94 (945/1,724 683172 683/1,72 68 (683/1,724 73172 73/1,72 7 (73/1,724 23172 23/1,72 (23/1,724 31757 317/57 31 (317/574 145/57 14557 14 (145/57 11257 112/57 11 (112/574 17 1, 287/5 2875 (287/5 94517 945/1,7 9 (945/1,72 68317 683/1,7 6 (683/1,72 7317 73/1,7 (73/1,72 2317 23/1,7 (23/1,72 3175 317/5 (317/57 145/5 1455 (145/5 1125 112/5 (112/57 287/ (287/ 9451 945/1, (945/1,7 6831 683/1, (683/1,7 731 73/1, (73/1,7 231 23/1, (23/1,7 317/ (317/5 145/ (145/ 112/ (112/5 (287 945/1 (945/1, 683/1 (683/1, 73/1 (73/1, 23/1 (23/1, (317/ (145 (112/ (28 945/ (945/1 683/ (683/1 73/ (73/1 23/ (23/1 (317 (14 (112 (2 (945/ (683/ (73/ (23/ (31 (1 (11 ( (945 (683 (73 (23 (3 (94 (68 (7 (9 (6

Resumo Estudos demonstram que o dispositivo selante de vasos DSV-LigaSure promove menor dano tecidual que o eletrocirúrgico bipolar e o aumento e diminuição das concentrações de proteínas e imunoglobulinas após a colecistectomia laparoscópica é esperado e transitório. Objetivou-se comparar os valores séricos das proteínas de fase aguda e imunoglobulinas de coelhos submetidos à dissecção da vesícula biliar utilizando eletrocirúrgico bipolar (pinça Maryland) e DSV-LigaSure para determinar qual dos dissectores apresentou menor alteração inflamatória. Vinte coelhos foram distribuídos em dois grupos com dez animais cada, sendo o Grupo 1 submetido à técnica de colecistectomia laparoscópica utilizando o eletrocirúrgico bipolar para dissecção e DSV-LigaSure para selar o ducto cístico e o Grupo 2, submetido à dissecção da vesícula biliar e selagem do ducto cístico utilizando o DSV-LigaSure. As proteínas de fase aguda e imunoglobulinas foram avaliadas nos dias três, sete e 15 do período pós-operatório. As concentrações séricas de fibrinogênio, transferrina, IgG, α1- glicoproteína ácida, PM 23000 Da e proteína C reativa não apresentaram diferenças significativas entre os grupos, apenas diferenças significativas entre os dias de avaliação no mesmo grupo. IgA, ceruloplasmina e haptoglobina não apresentaram diferenças estatísticas entre grupos e nem entre dias de avaliação. Apenas albumina apresentou diferença entre grupos, onde o Grupo 1 demonstrou menor concentração da proteína após 15 dias. Ambos dissectores apresentaram alterações nas proteínas de fase aguda, demonstrando que não houve superioridade na técnica utilizando o DSV-LigaSure. DSVLigaSure DSV LigaSure transitório Objetivouse Objetivou se pinça Maryland inflamatória cada 2 DSVLigaSure. LigaSure. três pósoperatório. pósoperatório pós operatório. operatório pós-operatório fibrinogênio transferrina IgG α1 α ácida 2300 grupo IgA 230 23

Abstract Studies have demonstrated that the LigaSure device causes less tissue damage than bipolar electrosurgery. Increases and decreases in protein and immunoglobulin concentrations after laparoscopic cholecystectomy are expected and transient. This study aimed to compare serum values of acute-phase proteins and immunoglobulins in rabbits undergoing gallbladder dissection using bipolar electrosurgery (Maryland forceps) and vessel sealing device (VSD) LigaSure. The objective was to determine which method resulted in less inflammatory change. Twenty rabbits were divided into two groups of ten each. Group 1 underwent laparoscopic cholecystectomy with bipolar electrosurgical forceps for dissection and LigaSure for sealing the cystic duct. Group 2 underwent dissection and cystic duct sealing using VSD-LigaSure only. Acute-phase proteins and immunoglobulins were evaluated on postoperative days three, seven, and fifteen. Serum concentrations of fibrinogen, transferrin, IgG, α1-acid glycoprotein, PM 23000 Da, and C-reactive protein (CRP) did not differ significantly between groups. However, significant differences were observed between evaluation days within the same group. IgA, ceruloplasmin, and haptoglobin were not statistically analyzed for either group or day comparisons. Only albumin levels differed between groups, with group 1 showing a lower protein concentration on day 15. Both methods caused changes in acute-phase proteins, indicating no significant advantage for using the LigaSure device. transient acutephase acute phase Maryland VSD (VSD change each VSDLigaSure only Acutephase Acute three seven fifteen fibrinogen transferrin IgG α1acid αacid α1 acid α glycoprotein 2300 Da Creactive C reactive CRP (CRP However IgA ceruloplasmin comparisons 15 230 23

ABSTRACT Purpose: The aim of this randomized study was to compare the complications and perioperative outcome of three different techniques of laparoscopic cholecystectomy (LC). Changes in the liver function test after LC techniques were investigated. Also, we compared the degree of postoperative adhesions and histopathological changes of the liver bed. Methods: Thirty rabbits were divided into three groups: group A) Fundus-first technique by Hook dissecting instrument and Roeder Slipknot applied for cystic duct (CD) ligation; group B) conventional technique by Maryland dissecting forceps and electrothermal bipolar vessel sealing (EBVS) for CD seal; group C) conventional technique by EBVS for gallbladder (GB) dissection and CD seal. Results: Group A presented a longer GB dissection time than groups B and C. GB perforation and bleeding from tissues adjacent to GB were similar among tested groups. Gamma-glutamyl transferase and alkaline phosphatase levels increased (p ≤ 0.05) on day 3 postoperatively in group A. By the 15th postoperative day, the enzymes returned to the preoperative values. Transient elevation of hepatic transaminases occurred after LC in all groups. Group A had a higher adherence score than groups B and C and was associated with the least predictable technique. Conclusions: LC can be performed using different techniques, although the use of EBVS is highly recommended. Purpose LC. . (LC) investigated Also bed Methods Fundusfirst Fundus first (CD ligation (EBVS seal (GB Results Gammaglutamyl Gamma glutamyl p 0.05 005 0 05 th values Conclusions recommended (LC 0.0 00 0.

RESUMO: A toracoscopia está substituindo as biópsias pulmonares abertas por ser menos invasiva. Usualmente a técnica é feita utilizando três portais e ligaduras feitas intra corporalmente. O objetivo deste estudo foi descrever a viabilidade e eficácia de uma nova ligadura com alça pré-amarrada e propor uma estratégia de acesso toracoscópico com dois portais para realizar biópsia pulmonar em pacientes com menos de 5kg. Dez coelhos foram posicionados em decúbito dorsal. A biópsia pulmonar por toracoscopia total foi feita pela abordagem combinada transdiafragmática e abordagem intercostal direita. Uma nova ligadura pré-amarrada foi colocada para realizar uma biópsia do lobo pulmonar caudal. Não foi realizada insuflação da cavidade torácica. O tempo total de cirurgia foi de 41,4 ± 14,5 min. Os procedimentos foram realizados sem complicações que impedissem o escorregamento ou aperto do nó ou que o soltassem após a biópsia pulmonar; não houve nenhuma intercorrência grave durante o procedimento cirúrgico. As amostras coletadas tinham tamanho médio 1x0,64x0,45 cm e foram consideradas satisfatórias de acordo com a avaliação histopatológica. As radiografias de tórax feitas antes e depois das cirurgias foram comparadas e não mostraram pneumotórax ou hemotórax. A necropsia confirmou que não ocorreu falha do nó no local da biópsia. Conclui-se que uso da nova ligadura com alça pré-amarrada é uma técnica segura e eficaz, evitando problemas com o tamanho limitado da cavidade torácica em pacientes pequenos. RESUMO invasiva corporalmente préamarrada pré amarrada 5kg kg dorsal direita caudal 414 41 4 41, 145 14 5 14, min cirúrgico 1x064x045 xx 1x0 64x0 45 x 1x0,64x0,4 histopatológica hemotórax Concluise Conclui se eficaz pequenos 1 1x064x04 1x 64x 1x0,64x0, 1x064x0 1x0,64x0 1x064x 1x0,64x

ABSTRACT: Thoracoscopy is replacing open lung biopsies because it is less invasive, usually the technique is done using three portals and intracorporeal suture technique. This study described the feasibility and efficacy of a novel pre tied loop ligature and to propose a thoracoscopic access strategy with two portals to perform lung biopsy in patients under 5 kg. Ten rabbits were positioned in dorsal recumbency. Total thoracoscopic lung biopsy was performed using a combined transdiaphragmatic approach and a right intercostal approach. A pre tied loop ligature was placed to perform a caudal lung lobe biopsy. Insufflation of the thoracic cavity was not performed. The total surgery time was 41.4 ± 14.5 min. The procedure was carried out free of complications that prevented slippage or tightening the knot or that made it come loose after the lung biopsy; there was no serious complication during the surgical procedure. The samples obtained averaged 1x0.64x0.45 cm (Length, Width, Depth) and were considered satisfactory according to the histopathologic evaluation. Thorax radiographs taken before and after the surgeries were compared and showed no pneumothorax or hemothorax. Necropsy confirmed no knot failure occurred at the biopsy site. The use of the novel pre tied loop ligature is a safe and effective technique, avoiding problems with the limited size of the thoracic cavity in small patients. ABSTRACT invasive kg recumbency 414 41 4 41. 145 14 14. min 1x064x045 xx 1x0 64x0 45 x 1x0.64x0.4 Length, Length (Length Width Depth evaluation hemothorax site 1 1x064x04 1x 64x 1x0.64x0. 1x064x0 1x0.64x0 1x064x 1x0.64x

Abstract The aim of this study was to evaluate the blood flow of the uterine artery, fetal aorta and umbilical artery in the physiological pregnancy of sheep by means of pulsed Doppler throughout the gestational period. Thirty Santa Inês ewes weighing between 45.4±4.3 kg and aged 2 to 5 years were selected. The evaluations were carried out weekly from the 3rd to the 21st gestational week. Peak systolic velocity (PSV), end diastolic velocity (EDV) and resistance index (RI) were obtained. Analysis of variance was performed, and the minimum significant comparison of means was obtained by the BH test with adjusted P<0.05. The results were expressed as mean ± standard error. For the fetal aorta, there was an increase in the EDV values and a decrease in the PSV and RI throughout pregnancy. For the uterine artery, PSV and EDV did not present significant variation, whereas the RI showed a reduction in the last week. Increased EDV values were found for the umbilical artery throughout pregnancy. For the PSV there was no significant difference, as the RI was reduced at the end of pregnancy. The results obtained are expected to contribute to a more complete understanding of the hemodynamic changes resulting from pregnancy.

Abstract Purpose To assess the feasibility of thoracoscopic transdiaphragmatic approach for biopsy of all lung lobes and to determine the optimal intercostal space (ICS) for biopsy of each lung lobe. Methods Ten rabbits were positioned in dorsal recumbency. Total thoracoscopy lung biopsy was made combined transdiaphragmatic approach and right ICS approaches. A camera port was made in the transdiaphragmatic approach and the instrument port was made of ICS 7 and ICS 9. A pre tied loop ligature was placed to performed a caudal lung lobe biopsy and to simulate biopsies of the others lung lobes. Results Biopsy of the cranial aspect of the right caudal lung lobe was performed at ICS 9. Simulated biopsy of the accessory lung lobe was performed at ICS 9. Simulated lung biopsy of the right cranial and middle lung lobes was performed at ICS 7. The caudal and dorsal aspect of the right caudal lung lobe was not visualized by telescope at transdiaphragmatic approach, and biopsy was not performed. Conclusions Thoracoscopic transdiaphragmatic approach for lung lobes biopsies was a feasible technique, except for the caudal aspect of the right caudal lung lobe. An ideal intercostal port for biopsy of each right lung lobe was determined.

Abstract Purpose To analyze changes in the thermal pattern in the skin graft receptor bed, after the use of therapeutic ultrasound through the thermographic images. Methods Eighteen Rattus norvegicus albinus Wistar, separated into two groups: GST groups (without tumor and without treatment with ultrasound) and GT (with tumor and treatment with ultrasound). In the GT group, induction of carcinogenesis was performed by single intradermal application of 0.05 ml DMBA at 0.5%, diluted in acetone. Subsequently, a technique of reconstructive grafting surgery of the mesh type was performed in both groups and treatment with therapeutic ultrasound was performed in the GT group the alternate day protocol at 3, 6, 10 and 15 days after the procedure. The thermographic evaluation occurred on days 3, 6, 10 and 15 after the grafting. Results There was a significant difference between the statistical evaluation of the temperature of the control group when compared to the treated group, on the different evaluation days (p <0.0001). Conclusion The thermographic analysis of the images was effective in evaluating the healing process, being the use of thermography feasible to evaluate changes in the thermal standard in the surgical bed, besides the beneficial effects of the US.

Abstract Purpose: The denervation of the intestine with benzalkonium chloride (BAC) reduces mortality and improves weight gain in rats with short bowel syndrome (SBS). Nevertheless, translating these promising findings from bench to bedside is not feasible because BAC promotes peritonitis and irreversible denervation which may be followed by an uncontrolled dilatation of the viscera. The use of botulinum toxin (BT) instead of BAC to achieve the denervation of the remaining small intestine in SBS could be an interesting option because it leads to a mild and transient denervation of the intestine. Methods: Here we evaluated the effects of the ileal denervation with BT in rats with SBS by verifying the body weight variation and intestinal morphological parameters. Four groups with 6 animals each were submitted to enterectomy with an ileal injection of saline (group E) or BT (group EBT). Control groups were submitted to simulated surgery with an ileal injection of BT (group BT) or saline (group C - control). Results: We observed that the treatment of the remaining ileum with BT completely reversed the weight loss associated to extensive small bowel resection. Conclusion: This may provide a new promising approach to the surgical treatment of SBS.

Resumo Biomateriais inovadores podem fornecer uma promissora nova direção para o tratamento de defeitos ósseos, estimulando um processo de reparo adequado, sem danos aos tecidos adjacentes. O objetivo deste estudo in vivo foi avaliar a biocompatibilidade e a capacidade osteoindutora de uma biomembrana e um scaffold compostos por colágeno e quitosana, contendo cimento de aluminato de cálcio. Dezoito coelhos (New Zealand White, Oryctolagus cuniculus) foram distribuídos de acordo com os períodos experimentais de análise (7, 15 e 30 dias). Quatro defeitos foram criados na calvaria dos coelhos, que foram individualmente preenchidos com a biomembrana, scaffold, coágulo (controle negativo) e osso autólogo (controle positivo). A avaliação histopatológica foi realizada em microscópio óptico em aumentos de 32´, 64´, 125´ e 320´. A resposta celular à inflamação e à formação de novo tecido ósseo foi quantificada utilizando um sistema de escore. O grupo da biomembrana apresentou maior resposta inflamatória no período de 15 dias, com diferença significativa para o grupo do osso autólogo (p<0,05). Não houve diferença estatística significante para a reação do tipo corpo estranho entre os grupos (p>0,05). Em relação à neoformação óssea, observou-se fechamento linear da área do defeito, que foi mais evidente no grupo em que se utilizou o osso autólogo. O grupo scaffold apresentou resultados semelhantes ao grupo do osso autólogo no período de 30 dias (p>0,05). Ambos os biomateriais testados apresentaram biocompatibilidade similar em comparação com os grupos controle. Além disso, a biomembrana e o scaffold apresentaram capacidade osteoindutora similar, estimulando o reparo ósseo ao longo dos intervalos de tempo experimentais.

Abstract Innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32´, 64´, 125´ and 320´ magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals.

This study evaluated the pH, calcium ion release and antimicrobial activity of EndoBinder (EB), containing different radiopacifiers: bismuth oxide (Bi2O3), zinc oxide (ZnO) or zirconium oxide (ZrO2), in comparison to MTA. For pH and calcium ion release tests, 5 specimens per group (n = 5) were immersed into 10 mL of distilled and deionized water at 37°C. After 2, 4, 12, 24, 48 h; 7, 14 and 28 days, the pH was measured and calcium ion release quantified in an atomic absorption spectrophotometer. For antimicrobial activity, the cements were tested against S. aureus, E. coli, E. faecalis and C. albicans, in triplicate. MTA presented higher values for pH and calcium ion release than the other groups, however, with no statistically significant difference after 28 days (p > 0.05); and the largest inhibition halos for all strains, with no significant difference (E. coli and E. faecalis) for pure EB and EB + Bi2O3 (p > 0.05). EB presented similar performance to that of MTA as regards pH and calcium ion release; however, when ZnO and ZrO2 were used, EB did not present antimicrobial activity against some strains.

O objetivo deste trabalho foi estudar a utilização da vídeo-otoscopia como método diagnóstico para otites externas de cães. Para tanto, foram utilizados 20 cães adultos. O grupo A foi formado por 10 animais (cinco machos e cinco fêmeas) provenientes do canil da Universidade Estadual Paulista, Campus de Jaboticabal, sem sinais clínicos aparentes de otite externa, e o grupo B foi formado por 10 animais (cinco machos e cinco fêmeas) encaminhados pelo setor de Clínica Médica do Hospital Veterinário da faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Campus de Jaboticabal, apresentando sinais clínicos de otite. Para realização dos exames, todos os animais foram submetidos ao mesmo protocolo anestésico (acepromazina na dose de 0,1mg kg-1 e tiletamina-zolazepan na dose de 10mg kg-1). Foram diagnosticadas três otites unilaterais e 13 bilaterais, e dentro dos achados estão secreção purulenta; epitélio ulcerado, hiperêmico ou hiperplásico; estenose do meato e alterações da membrana timpânica. A vídeo-otoscopia mostrou ser um método efetivo e um procedimento fundamental no auxílio diagnóstico e prognóstico das desordens auriculares.

The objective of this study was the use of video-otoscopy as a method to diagnose external otitis in dogs. Hence 20 adult dogs were used. The group A was formed by 10 animals (5 males and 5 females) with no apparent symptoms of the external otitis and the group B, formed by 10 animals (5 males and 5 females) sent by the Medical Clinics section of the Veterinary Hospital at the Universidade Estadual Paulista - Câmpus Jaboticabal, with otitis symptoms. All the animals tested have undergone the same anesthesic protocol (acepromazine in a dosis of 0,1mg kg-1 and tiletamine-zolazepan in a dosis of 10mg kg-1). Three unilateral and 13 bi-lateral otitis were diagnosed and the findings include purulent secretion; ulcerated, hyperemic or hyperplasic epithelium; meathus stenosis and alterations in the timpanic membrane. Video-otoscopy has shown to be an effective and a fundamental procedure to help diagnosing and prognosting auricular disorders.