Mycorrhizal fungus inoculum produced on-farm can be used during production of woody plant seedlings to reduce costs associated with purchase of commercial inoculant and fertilization. This study aimed to test the efficiency of a mycorrhizal inoculant produced on-farm to promote growth and nutrition of woody species in combination with different levels of phosphorus. Plants were submitted to different treatments of phosphorus (0, 40 and 80 mg P/dm3) and mycorrhizal inoculation (uninoculated, and inoculation with Rhizophagus clarus [Rc] or Claroideoglomus etunicatum [Ce]). Species included were Luehea divaricata, Centrolobium robustum, Schinus terebinthifolius, Garcinia gardneriana, Cedrella fissilis, and Lafoensia pacari. The inoculum was produced using the on-farm methodology. Mycorrhizal colonization of plants inoculated with Rc and Ce ranged from 44.8 to 74.8%, except forGarcinia gardneriana. Inoculation treatment increased plant height and stem diameter of Luehea divaricata, Centrolobium robustum and Cedrella fissilis while phosphorus, inoculation and the interaction affected these parameters for G. gardneriana and Lafoensia pacari. Shoot biomass increased significantly with inoculation treatment in four species. For most species, mycorrhizal fungus inoculation and the addition of phosphorus increased the shoot phosphorus content. Mycorrhizal fungus inoculum produced on-farm successfully colonized tree seedlings and improved growth and/or nutrition under nursery conditions, producing seedlings useful for revegetation of degraded lands.