Abstract Synthesis, characterization and biological activity of the Cu(II)-1,3-PDTA complex was performed by two synthetic routes: sodium and carbonate pathways. The Cu (II)-1,3-PDTA complex can be easily produced by the 1,3-PDTA binder, commercially available as Trilon F (BASF) which is available as sodium or acid salt for the coordination of Cu (II). The biological activity of the Cu (II)-1,3-PDTA [Cu (II)-PDTA] complex was performed to analyze the activity against the bacterium Erwinia chrysanthemi Bancroft. The bacterium is the causative agent of soft rot in the parsley maniac (Arracacia xanthorrhiza Bancroft). The bacterium was isolated from parsley manioc infected with soft rot. The metal complex sensitivity tests were performed by diffusion antibiogram. The mean values of the halos and standard deviations of the zones of inhibition were obtained for the [Cu (II) (PDTA)] and Streptomycin complexes. The concentrations of the complexes evaluated were 10-3, 10-2 and 10-1 M (equivalent to 36.3 g L-1 (3.63%), 3.63 g L-1 (0.363%) and 0.363 g L-1 (0.0363%), respectively). The halos were 25.0 ± 1.2 mm for control. For [Cu (II) (PDTA)] complexes, the obtained values were 9.5 ± 0.6, 15.0 ± 0.8 and 24.0 ± 0.8 via sodium and 12.0 ± 1.6, 25.0 ± 0.8 and 31.0 ± 1.9 via carbonate, for 10-3, 10-2 and 10-1 M, respectively. The results showed that the bactericidal activity of the Cu (II)-1,3-PDTA complex obtained by the two synthetic routes are adequate for the control of Erwinia chrysanthemi Bancroft.

ABSTRACT Natural additives, particularly essential oils, have gained widespread recognition for their role in enhancing the attributes of natural edible polymers. Comprising a wealth of hydrophobic and volatile compounds, essential oils exhibit notable antioxidant and antimicrobial properties owing to their rich composition of terpenes and aromatic constituents. This review underscores the multifaceted biological properties of essential oils, encompassing their incorporation into films, edible coatings, and nanoencapsulated materials. The effect of utilizing several essential oils as natural additives in combination with different raw materials and plasticizers was compared to the evaluation of their impact on the material properties of films and edible coatings, offering an in-depth analysis of the specific essential oil variants featured in the recent literature. Among the essential oils reviewed, those derived from clove, cinnamon, and oregano emerge as the predominant choices, representing some of the most promising natural additives for biodegradable packaging. Nanoencapsulation techniques have also expanded the role of essential oils in sustainable food packaging by increasing their stability. polymers compounds constituents coatings indepth depth literature reviewed clove cinnamon choices stability

Resumen Antecedentes: Muchos híbridos de peces (i.e., obtenidos al cruzar dos especies) se producen en lugar de especies puras debido a su mejor tasa de crecimiento y/o aceptación de alimentos formulados. Sin embargo, pocos estudios evalúan y comparan sus carnes y aceptación, incluyendo, p. ej., para lo surubí híbrido amazónico (Pseudoplatystoma reticulatum x Leiarius marmoratus) y lo surubí híbrido real (Pseudoplatystoma corruscans x L. marmoratus). Objetivo: Así, el objetivo de este trabajo fue evaluar las características físicas, químicas, microbiológicas y sensoriales de filetes sorubines híbridos amazónico y real. Método: Se midió composición proximal, color instrumental, capacidad de retención de agua, pierdas por cocción y fuerza de corte. Se realizaron análisis microbiológicos de los filetes para Salmonella sp. y Staphylococcus aureus para garantizar la seguridad alimentaria durante el análisis sensorial. Resultados: Los filetes presentaron una excelente calidad, siendo clasificados en la categoría A (lípidos por debajo del 5% y proteínas entre 15 y 20%). No hubo diferencia significativa (p>0.05) entre los filetes evaluados para varios parámetros: las capacidades de retención de agua fueron 33.72 y 34.67%, las pierdas por cocción fueron 14.93 y 13.41%, las fuerzas de corte fueron 2.21 y 1.74 kgf y las luminosidades fueron 49.61 y 45.04 para los filetes de sorubim híbrido amazónico y sorubim híbrido real, respectivamente. Discusión: Hubo una relación evidente entre las capacidades de retención de agua y las fuerzas de corte. Los filetes de sorubim híbridos amazónicos presentaron menor intensidad de rojo. Sin embargo, no hubo diferencia sensorial entre los híbridos, y ambos tuvieron una tasa de aceptación superior al 80%. Conclusión: La hibridación no altera las características de los filetes de pescado.

Abstract Background: Many fish hybrids (i.e., obtained by crossing two species) are produced rather than pure species because of their better growth rate and/or acceptance of formulated feed. However, few studies evaluate and compare their meats and acceptance, including, e.g., for the Amazon hybrid sorubim (Pseudoplatystoma reticulatum x Leiarius marmoratus) and the real hybrid sorubim (Pseudoplatystoma corruscans x L. marmoratus). Objective: Thus, this work aimed to evaluate the physical, chemical, microbiological, and sensory characteristics of fillets from the Amazon and real hybrid sorubins. Method: Proximate composition, instrumental color, water holding capacity, cooking losses, and shear force were measured. Microbiological analyses of the fillets were carried out for Salmonella sp. and Staphylococcus aureus to ensure food safety during sensory analysis. Results: Fillets presented excellent quality, classified in category A (lipids below 5% and proteins between 15 and 20%). There was no significant difference (p>0.05) between the evaluated fillets for several parameters: the average water holding capacities were 33.72 and 34.67%, the cooking losses were 14.93 and 13.41%, the shear forces were 2.21 and 1.74 kgf, and the luminosities were 49.61 and 45.04 for the fillets of Amazonian hybrid sorubim and real hybrid sorubim, respectively. Discussion: There was an evident relationship between water-holding capacities and shear forces. Amazonian hybrid sorubim fillets presented lower intensity of red. However, there was no sensory difference between the hybrids, and both had an acceptance rate above 80%. Conclusion: The hybridization does not alter the characteristics of the fish fillets.

ABSTRACT Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.

Background β-Glucosidases catalyze the hydrolysis of cellobiose and cellodextrins, releasing glucose as the main product. This enzyme is used in the food, pharmaceutical, and biofuel industries. The aim of this work is to improve the β-glucosidase production by the fungus Lichtheimia ramosa by solid-state fermentation (SSF) using various agroindustrial residues and to evaluate the catalytic properties of this enzyme. Results A high production of β-glucosidase, about 274 U/g of dry substrate (or 27.4 U/mL), was obtained by cultivating the fungus on wheat bran with 65% of initial substrate moisture, at 96 h of incubation at 35°C. The enzymatic extract also exhibited carboxymethylcellulase (CMCase), xylanase, and β-xylosidase activities. The optimal activity of β-glucosidase was observed at pH 5.5 and 65°C and was stable over a pH range of 3.5-10.5. The enzyme maintained its activity (about 98% residual activity) after 1 h at 55°C. The enzyme was subject to reversible competitive inhibition with glucose and showed high catalytic activity in solutions containing up to 10% of ethanol. Conclusions β-Glucosidase characteristics associated with its ability to hydrolyze cellobiose, underscore the utility of this enzyme in diverse industrial processes.

Background: Enzyme production by solid state bioprocess (SSB) using residues as substrate for microorganisms is an alternative for costs reduction and to avoid their disposal into environment. The aim of this work was to evaluate the physiology of the fungus Lichtheimia ramosa in terms of microbial growth and production of amylases, β-glucosidases, carboxymethylcellulase (CMCase), and xylanases, via SSB, utilizing wastes of the Brazilian savannah fruits bocaiuva (Acrocomia aculeata), guavira (Campomanesia pubescens) and pequi (Caryocar brasiliense) as substrate at different temperatures (25, 30, and 35ºC) during 168 hrs. Results: Samples were taken every 24 hrs, which resulted in 8-points kinetic experiments to determine microbiological and enzymatic contents. The best substrate for β-glucosidase activity was pequi waste after 48 hrs at 30ºC (0.061 U/mL). For amylase activity, bocaiuva presented itself as the best substrate after 96 hrs at 30ºC (0.925 U/mL). CMCase activity was higher in guavira waste after 96 hrs at 35ºC (0.787 U/mL). However, the activity was more expressive for xylanase in substrate composed of bocaiuva residue after 144 hrs at 35ºC (1.802 U/mL). Conclusions: It was concluded that best growth condition for L. ramosa is at 35ºC for all substrates and that xylanase is the enzyme with more potential in SSB, considering the studied Brazilian savannah fruit wastes.

Gelatin was extracted from the skin of tilapia (Oreochromis urolepis hornorum) and characterized according to its physical and chemical properties. It had pH 4.66, which is slightly higher than the values reported for gelatins processed by acid solubilization. In general, the ionic content was low, and the average yield of the process was 5.10 g/100 g. The proximal composition of the gelatin was similar to that of the commercial gelatins, with slightly higher moisture content. The tilapia skin gelatin had whitish-yellow color and average turbidity of 67 NTU.

A presença de microrganismos em produtos alimentícios durante produção, armazenamento, transporte e embalagem é inevitável. Sendo assim, conhecer o comportamento do crescimento microbiano é muito importante para a segurança alimentar e estimativa da vida útil. Neste trabalho foi simulada a cadeia de abastecimento de peitos de frangos cru, salgado e cozido durante 20 dias a -18 ± 0,5 ºC (simulação da expedição industrial no Brasil e transporte por navio até a Europa) e, após o descongelamento, 21 dias a 4 ± 0,5 ºC (simulação da vida útil em supermercado). Os produtos foram analisados quanto a Pseudomonas spp., Salmonella spp., Listeria monocytogenes, Staphylococcus spp. e microrganismos viáveis totais (mesófilos e psicrotróficos). As análises de contagem em placas foram seguidas por testes bioquímicos clássicos para a confirmação de colônias típicas. Em nenhuma das amostras foi detectada a presença de Salmonella spp. ou de Listeria monocytogenes. Em termos de contagem de colônias viáveis totais, durante os primeiros 20 dias (a -18 ºC) a presença de microrganismos se manteve estável em baixos níveis de detecção. Depois do descongelamento, curvas de crescimento microbiano foram observadas. De acordo com os parâmetros microbiológicos de segurança, tempos entre 9 e 11 dias a 4 ºC mostraram-se como os limites para garantir a qualidade destes produtos

The presence of the microbial organisms in food products during the production, storage, transport, and packaging is unavoidable. Therefore, the knowledge of the microbial growth behavior is very important for the food safety and shelf life assessment. In this work, a chicken supply chain was simulated for raw, salted, and cooked chicken breasts for 20 days at -18 ± 0.5 ºC (simulation of industry expedition in Brazil and transport by ship to Europe) and for defrosted chicken breasts for 21 days at 4 ± 0.5 ºC (simulation of the shelf life in supermarket). The products were analyzed for Pseudomonas spp., Salmonella spp., Lysteria monocytogenes, Staphylococcus spp. and total viable counts (mesophilic and psicrotrophic microorganisms). The counting plate analyses were followed by classical biochemical tests for typical colonies confirmation. None of the samples was positive for Salmonella spp. and Lysteria monocytogenes. In terms of total viable colonies counting, during the first 20 days (-18 ºC), the presence of microorganisms was kept stable at low levels of detection. After defrosting, microbial growth curves were observed. According to the safety parameters, 9 to 11 days at 4 ºC proved the best time limit to guarantee the quality of those products