ABSTRACT In the Brazilian Cerrado, phytonematodes, phytonematodes are a challenge for agriculture, and the association of forms of control is the best strategy to be used in coexistence with this pathogen. Faced with this problem, this study aimed to evaluate the efficiency of cover crops and biocontrol agents in the management of nematodes in soybean. For each nematode species under study (Meloidogyne incognita and Pratylenchus brachyurus), two experiments were carried out under greenhouse conditions in a completely randomized design arranged in a 6 × 2 factorial scheme with eight replications. Six cover crops (millet ADRG 9050, millet ADR 300, Urochloa ruziziensis, Crotalaria ochroleuca, C. spectabilis, and corn hybrid DKB 290) and two treatments (treated or not with Bacillus subtilis and B. methylotrophicus - 1×106 CFU per seed) were evaluated. The cover crops were sown in soil infested with nematodes and then soybeans were sown under the straw, and cultivated for 45 and 60 days, for M. incognita and P. brachyurus, respectively. Fresh root mass, total nematode population, and the number of nematodes per gram of root were evaluated. The association of microorganisms with C. spectabilis, U. ruziziensis, and millet ADR 300 conferred an additional effect in reducing the population of P. brachyurus. For M. incognita, the association was successful only for combining C. spectabilis with B. methylotrophicus. DKB 290 corn, when treated with B. methylotrophicus, had the nematode population per gram of soybean root reduced by 90% compared to plants that did not receive biological treatment. Cerrado agriculture pathogen problem Meloidogyne brachyurus , brachyurus) replications 9050 ruziziensis ochroleuca C B 1106 1 106 1×10 seed evaluated straw 4 days M P respectively mass U 30 29 90 treatment 905 110 10 1×1 3 9 11 1×

RESUMO No Cerrado brasileiro, os fitonematoides são um desafio para agricultura, sendo a associação de formas de controle a melhor estratégia a ser utilizada na convivência com esse patógeno. Diante dessa problemática, o objetivo desse trabalho foi avaliar a eficiência de plantas de cobertura e agentes de biocontrole no manejo de nematoides na cultura da soja. Foram desenvolvidos dois ensaios em condições de casa de vegetação para os nematoides Meloidogyne incognita e Pratylenchus brachyurus, em delineamento inteiramente casualizado, em esquema fatorial 6 (plantas de cobertura: milheto ADRG 9050, milheto ADR 300, Urochloa ruziziensis, Crotalaria ochroleuca, C. spectabilis e milho DKB 290) × 2 (tratados ou não com Bacillus subtilis e B. methylotrophicus - 1×106 UFC por semente), com oito repetições. As plantas de cobertura foram semeadas em solo infestado com nematoides e na sequência semeada soja sob a palha, e cultivada por 45 e 60 dias, para M. incognita e P. brachyurus, respectivamente. Foram avaliadas a massa fresca de raízes e o número de nematoides totais e por grama de raiz. A associação dos microrganismos com C. spectabilis, U. ruziziensis e milheto ADR 300 conferiram efeito adicional na redução da população de P. brachyurus. Para a espécie M. incognita a associação foi bem sucedida apenas para a combinação da C. spectabilis com B. methylotrophicus. O milho DKB 290, quando tratado com B. methylotrophicus, teve a população do nematoide por grama de raiz da soja reduzida em 90 % em comparação às plantas que não receberam o tratamento biológico. brasileiro agricultura patógeno problemática brachyurus casualizado 9050 ochroleuca C 290 tratados B 1106 1 106 1×10 semente, semente , semente) repetições palha 4 dias M P respectivamente U 30 9 biológico 905 29 110 10 1×1 3 11 1×

Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics. Objective HyperactivityInattention Hyperactivity Inattention (HI COVID19 COVID 19 COVID-1 Methods crosssectional cross sectional 34 10 sociodemographic socio demographic selfrated self rated surveys SDQ, , (SDQ) PSQI, PSQI (PSQI) 40 4 0 4. PedsQL4.0. PedsQL40 PedsQL4.0 . PedsQL4 (PedsQL4.0) Results 110/343 110343 110 (110/34 32% 32 [32% vs 38108 38 38/10 35%, 35 35% [35%] 0.548, 0548 0.548 548 0.548) hyperactivityinattention hyperactivity inattention 79/343 79343 79 (79/34 23% 23 [23% 29108 29 29/10 27%, 27 27% [27%] 0.417. 0417 0.417 417 0.417) Odds OR 3.76 376 3 76 3.76] 95 (95 2.00–7.05 200705 2 00 7 05 0.001, 0001 0.001 001 0.001) 2.05 205 1.08–3.88 108388 1 08 88 0.028 0028 028 2.17 217 17 1.12–4.19 112419 12 0.021 0021 021 0.95 095 0.93–0.96 093096 93 96 0.0001. 00001 0.0001 0.0001) 0.97 097 97 0.95–0.99 095099 99 0.010, 0010 0.010 010 0.010] 0.39 039 39 0.19–0.79 019079 0.021, 0.35 035 0.16–0.77 016077 16 77 0.026 0026 026 Conclusion pandemics COVID1 COVID- (SDQ (PSQI PedsQL4. (PedsQL4.0 110/34 11034 11 (110/3 [32 3810 38/1 [35% 054 0.54 54 79/34 7934 (79/3 [23 2910 29/1 [27% 041 0.41 41 3.7 37 9 (9 2.00–7.0 20070 000 0.00 2.0 20 1.08–3.8 10838 8 0.02 002 02 2.1 21 1.12–4.1 11241 0.9 09 0.93–0.9 09309 0000 0.000 0.95–0.9 09509 0.01 01 0.3 03 0.19–0.7 01907 0.16–0.7 01607 (PedsQL4. 110/3 1103 (110/ [3 381 38/ [35 0.5 5 79/3 793 (79/ [2 291 29/ [27 04 0.4 3. ( 2.00–7. 2007 0.0 2. 1.08–3. 1083 1.12–4. 1124 0. 0.93–0. 0930 0.95–0. 0950 0.19–0. 0190 0.16–0. 0160 (PedsQL4 110/ (110 [ 79/ (79 2.00–7 200 1.08–3 1.12–4 112 0.93–0 093 0.95–0 0.19–0 019 0.16–0 016 (PedsQL (11 (7 2.00– 1.08– 1.12– 0.93– 0.95– 0.19– 0.16– (1 2.00 1.08 1.12 0.93 0.19 0.16 1.0 1.1 0.1 1.

Objective: Clinical and biological correlates of resilience in major depressive disorder are scarce. We aimed to investigate the effect of the Val66Met polymorphism in the BDNF gene on resilience scores in major depressive disorder patients and evaluate the polymorphism’s moderation effect on resilience scores in response to cognitive therapy. Method: A total of 106 major depressive disorder patients were enrolled in this clinical randomized study. The Resilience Scale and the Hamilton Rating Scale for Depression were applied at baseline, post-treatment, and at six months of follow-up. Blood samples were obtained at baseline for molecular analysis. Results: The baseline resilience scores were higher in patients with the Met allele (114.6±17.6) than in those with the Val/Val genotype (104.04±21.05; p = 0.037). Cognitive therapy treatment increased resilience scores (p ≤ 0.001) and decreased depressive symptoms (p ≤ 0.001). In the mixed-effect model, the Val/Val genotype represented a decrease in resilience scores (t218 = -1.98; p = 0.048), and the Val66Met polymorphism interacted with sex to predict an increase in total resilience scores during cognitive treatment (t218 = 2.69; p = 0.008). Conclusion: Our results indicate that cognitive therapy intervention could improve resilience in follow-up, considering that gender and genetic susceptibility are predicted by the Val66Met polymorphism.

RESUMO Objetivou-se com este estudo determinar o coeficiente de cultura (kc) e respectivo consumo de água da cultura do pimentão, híbrido Magali R., cultivado em ambiente protegido no período de maio a setembro de 2015. A evapotranspiração de referência foi estimada pelo método de Penman-Monteith Modificado com dados coletados em estação meteorológica automática instalada no interior da casa de vegetação localizada na Universidade Estadual de Maringá, no município de Maringá, PR, (23º 25’ 57’’ S e 51º 57’ 8’’ O, e altitude de 542 m). A evapotranspiração da cultura foi mensurada por meio de lisímetros de lençol freático constante. O consumo total de água da cultura do pimentão foi de 282 mm, com consumo médio de 2,35 mm d-1, atingindo a evapotranspiração máxima da cultura de 5,37 mm d-1. Os valores do coeficiente de cultivo encontrados para o pimentão foram de 0,86; 1,55 e 1,4 para o estádio inicial, intermediário e final, respectivamente, sendo mais elevados do que os recomendados pela FAO.

ABSTRACT This study aimed to determine the crop coefficient (kc) and respective water consumption of the Magali R. bell pepper hybrid cultivated in a protected environment from May to September 2015. Reference evapotranspiration was estimated using the Modified Penman-Monteith method and data were collected from an automatic weather station installed inside the greenhouse at the Universidade Estadual de Maringá, in the municipality of Maringá, PR, Brazil (23° 25’ 57’’ S, 51° 57’ 8’’ W, and altitude of 542 m). Evapotranspiration was measured using constant water table lysimeters. The total water consumption of the bell pepper crop was 282 mm and the average consumption was 2.35 mm d-1. The maximum evapotranspiration was 5.37 mm d-1. Crop coefficient values for the bell pepper were 0.86, 1.55, and 1.4 for the initial, intermediate, and final stages, respectively, which were higher than those recommended by the Food and Agriculture Organization.

A combined phytochemical, crystallographic and biological study of Worsleya procera roots was performed. Fifteen alkaloids were identified by gas chromatography mass spectrometry (GC-MS) and seven of them were isolated. The structures of the alkaloids were elucidated by spectroscopic methods, and a detailed crystallographic study of tazettine was carried out. The isolated alkaloids and the obtained extracts were tested in vitro against Plasmodium falciparum (3D7 and K1 strains) and human hepatocarcinoma cells (HepG2) to assess their antiplasmodial and cytotoxic effects, respectively. One of the isolated alkaloid derivatives, lycorine, exhibited antiplasmodial activity against both sensitive (3D7) and resistant (K1) parasite strains in the low micromolar range (half-maximal sample inhibitory concentration (IC50) values of 2.5 and 3.1 µM, respectively) and displayed a low cytotoxicity profile, with a selectivity index greater than 100. Our findings indicate that lycorine is a hit for antimalarial drug discovery.

Objective: To identify the association of metabolic syndrome (MetS) and psychiatric disorders in young adults in southern Brazil. Methods: This population based cross-sectional study involved a total of 1,023 young adults between the ages of 21 and 32 years. Current episodes of psychiatric disorders were assessed using the Mini International Neuropsychiatric Interview – Plus version. MetS was evaluated using the National Cholesterol Education Program Adult Treatment Panel III (NCEP/ATP III). Results: Of the 1,023 participants, 24.3% were identified with MetS, 13.5% were diagnosed with anxiety disorders, 7.5% with current depression, 3.9% with bipolar disorders and 10.1% were at risk of suicide. MetS was associated with ethnicity (p = 0.022), excess weight (p < 0.001), current anxiety disorders (p < 0.001), current mood disorders (bipolar disorder in mood episode and current depression) (p < 0.001), and suicide risk (p < 0.001). Conclusions: MetS was associated with psychiatric disorders. Awareness of factors associated with MetS can help identify high-risk individuals and stimulate disease prevention and control programs, as well as lifestyle changes.

Objective: To evaluate the prevalence of alcohol abuse and/or dependence in a population-based sample of young adults and assess the prevalence of comorbid mood disorders, anxiety, and suicide risk in this population. Methods: This cross-sectional, population-based study enrolled 1,953 young adults aged 18-35 years. The CAGE questionnaire was used to screen for alcohol abuse and/or dependence, with CAGE scores ≥ 2 considered positive. Psychiatric disorders were investigated through the structured Mini International Neuropsychiatric Interview (MINI). Results: Alcohol abuse and/or dependence was identified in 187 (9.60%) individuals (5.10% among women and 15.20% among men). Alcohol abuse and/or dependence were more prevalent among men than women, as well as among those who used tobacco, illicit drugs or presented with anxiety disorder, mood disorder, and suicide risk. Conclusion: These findings suggest that alcohol abuse and/or dependence are consistently associated with a higher prevalence of psychiatric comorbidities, could be considered important predictors of other psychiatric disorders, and deserve greater public heath attention, pointing to the need for alcohol abuse prevention programs.

Objetivou-se com este estudo avaliar as características físico-químicas de parentais e híbridos experimentais de melão rendilhado, desenvolvidos na Universidade Estadual Paulista (UNESP), em Jaboticabal. Foram utilizadas seis linhagens (Jab-3; Jab-7; Jab-11; Jab-18; Jab-9 e Jab-20) pertencentes ao programa de melhoramento genético do meloeiro rendilhado da UNESP em Jaboticabal e, todas as combinações híbridas possíveis entre essas, inclusive os recíprocos. Foi utilizado o delineamento em blocos casualizados com três repetições. Dos 38 tratamentos, fizeram parte seis linhagens, 15 híbridos, 15 híbridos recíprocos e duas cultivares comerciais (Bônus nº2 e Louis). Foram avaliadas massa do fruto, espessura da polpa, espessura da casca, rendilhamento da casca, desprendimento de semente, sólidos solúveis, acidez titulável, pH, índice de maturação, firmeza da polpa, e, Vitamina C. A massa mínima de 0,8 kg por fruto de melão rendilhado e, sólidos solúveis de no mínimo 10°Brix subsidiaram a identificação dos genótipos Jab-9 x Jab-3, Jab-18 x Jab-20, Jab-18 x Jab-11, Jab-7 x Jab-20, Jab-7 x Jab-11, Jab-20 x Jab-7, e Jab-3 x Jab-20 como promissores, devendo os mesmos serem avaliados nas principais regiões produtoras de melão rendilhado e em diferentes épocas de cultivo.

The objective of this study was to evaluate the physicochemical characteristics of parents and experimental hybrids of net melon improvement program developed in the Universidade Estadual Paulista (UNESP), in Jaboticabal, São Paulo state, Brazil. Six net melon lineages (Jab-3, Jab-7, Jab-11, Jab-18, Jab-9, and Jab-20), belonging to the breeding program of melon from the UNESP were used and all their hybrid combinations as well as their reciprocal crosses. We used the randomized blocks design, with three replications and 38 treatments. From these 38 treatments [six lines, 15 hybrids, 15 reciprocal crosses and two commercial cultivars (Bônus nº2 and Louis)] we evaluated following physicochemical characteristics: fruit mass, pulp thickness, fruit coat thickness, netting degree of the coat, seed looseness, soluble solids, titratable acidity, pH, maturation index, pulp firmness, and Vitamin C. The minimum mass of 0.8 kg per fruit of net melon and the minimum of 10°Brix soluble solids allowed to identify the genotypes: Jab-9 x Jab-3, Jab-18 x Jab-20, Jab-18 x Jab-11, Jab-7 x Jab-20, Jab-7 x Jab-11, Jab-20 x Jab-7, and, Jab-3 x Jab-20, as promising. These genotypes are therefore suitable for participating in assessment tests in the main net melon producing areas of Brazil and on different planting dates.

Objetivou-se, com este trabalho, avaliar índices de crescimento, fisiológicos e de produção, de quatro híbridos de meloeiros cultivados com e sem cobertura do solo e três freqüências de irrigação. Utilizaram-se dois experimentos conduzidos no período de junho a setembro de 2001, em Mossoró, RN. Num se cultivou usando solo nu e noutro, usando cobertura com filme de polietileno. Adotou-se o delineamento experimental em blocos completos casualizados com duas repetições por bloco. Utilizaram-se três freqüências de irrigação, com cada uma aplicada num bloco experimental. Estudaram-se os híbridos de melão cantaloupe, denominados PX3912947 (H1), Vera cruz (H2), Trusty (H3) e Torreon (H4). Maior área foliar foi obtida nos híbridos H1 e H2, independente da cobertura e freqüência de irrigação. No sistema de plantio com cobertura do solo, a cultura do meloeiro aumentou o peso seco da parte vegetativa sem os frutos e suportou turno de regas maiores. Os híbridos H2 e H4 obtiveram, juntos, maior taxa de crescimento absoluto no solo descoberto, enquanto apenas o híbrido H4 alcançou maior taxa de crescimento relativo, neste solo. Os híbridos H1 e H3 produziram frutos grandes fora do padrão. A cobertura do solo proporcionou maior produtividade e peso médio dos frutos.

The objective of this study was to evaluate growth, physiologic and production indices of four hybrids of melon crop cultivated with and without soil covering and three irrigation frequencies. Two experiments were conducted during the period of June to September, 2001, in Mossoró, RN, using soil with and without a covering of polyethylene film. The experimental design was in completely randomized blocks with two repetitions per block. Three irrigation frequencies were used, with each one applied in an experimental block. The hybrids of melon cantaloupe, denominated PX3912947 (H1), Vera Cruz (H2), Trusty (H3) and Torreon (H4) were studied. Larger foliar area was obtained in the hybrids H1 and H2, independent of soil covering and irrigation frequency. In the planting system with soil covering, the melon crop increased the dry weight of aerial parts without fruits and tolerated higher irrigation frequencies. The hybrids H2 and H4 presented higher rates of absolute growth in bare soil, while the hybrid H4 reached a higher relative growth rate in this soil. The hybrids H1 and H3 produced large fruits beyond the standard. The covering of soil resulted in higher productivity and greater mean weight of fruits.

Neste estudo verificamos a influência de galhas de ramos induzida por uma espécie não identificada de Apion sp. (Brentidae, Apioninae) sobre a área foliar da planta hospedeira Diospyros hispida (Ebenaceae) e na resistência induzida contra galhas de folhas de um Cecidomyiidae (Diptera). Este estudo foi conduzido em uma vegetação de cerrado na Serra do Cipó, sudeste do Brasil. Embora o número de folhas produzidas nos ramos galhados e não galhados não tenha diferido estaticamente (p>0,05), a presença das galhas de Apion sp. influenciou a área foliar nos ramos atacados de D. hispida. Folhas de ramos com galhas foram aproximadamente 50% menores quando comparadas a folhas em ramos saudáveis. A média de galhas foliares do Cecidomyiideae que se formaram com sucesso em folhas de ramos saudáveis foi maior quando comparado à média de galhas bem sucedidas em folhas de ramos galhados pelo Apion sp.. O mesmo padrão foi encontrado para a abundância de reações hipersensibilidade contra a formação de galhas do cecidomyiídeo. Portanto, a habilidade do cecidomyiídeo de induzir galhas não foi influenciada pelas galhas de Apion sp..

We addressed the influence of the stem galls induced by an unidentified species of Apion sensu lato (Brentidae, Apioninae) on the host plant, Diospyros hispida (Ebenaceae) leaf area and induced resistance against a Cecidomyiidae (Diptera) leaf galls. The study was performed in a cerrado vegetation in Serra do Cipó, southeastern Brazil. Although the number of leaves produced on galled and ungalled shoots did not differ statically (p>0.05), the presence of the apionid galls influenced the area of the leaves on the attacked shoots of D. hispida. Leaves on galled stems were approximately 50% smaller compared to leaves in healthy stems. The average of the cecidomyiid leaf galls successfully induced on healthy shoots was higher compared to galls successfully induced on shoots galled by the apionid. The same pattern was found for the abundance of hypersensitive reactions against the cedidomyiid gall induction. Therefore, the ability of the cecidomyiid to successfully induce galls was not influenced by the apionid galler.

O experimento foi conduzido na horta da ESAM, de março a abril de 2004, com o objetivo de avaliar o desempenho de cultivares da alface em túneis baixos de agrotêxtil. O delineamento experimental utilizado foi de blocos casualizados completos em esquema fatorial 2 x 3, com quatro repetições. Os tratamentos foram constituídos pela combinação de duas cultivares de alface (Tainá e Babá de Verão) e três tipos de cobertura (dois túneis baixos de 50 cm de altura de agrotêxtil branco com gramatura de 13 g/m² e 40 g/m² e sem cobertura). Avaliou-se a altura e diâmetro de plantas, número de folhas por planta, massa seca da parte aérea e produtividade. A cultivar Babá de Verão apresentou melhor desempenho em altura de plantas, número de folhas e produtividade. O túnel baixo de agrotêxtil branco com gramatura de 40 g/m² promoveu maior altura, diâmetro e produtividade de alface.

The yield of lettuce cultivated under low tunnels of agricultural textile was evaluated in Mossoró, Rio Grande do Norte State, Brazil. The experiment was carried out from March to April of 2004, in a randomized complete blocks design with three treatments and four replications. The treatments were: low tunnel (with 50 cm of height) of white agricultural textile with basic weight of 40 g/m²; low tunnel (with 50 cm of height) of white agricultural textile with basic weight of 13 g/m² and control (treatment with no coverage). Evaluations of plant height and diameter, number of leaves per plant, shoot dry matter and yield were done. Cultivar Babá de Verão presented greater plant height, number of leaves per plant and yield. The low tunnel of agricultural textile with basic weight of 40 g/m² presented superior results of plant height, diameter and yield.