RESUMO A mastite clínica bovina causada por Staphylococcus spp. é uma doença grave e generalizada no mundo da pecuária leiteira. A terapia antimicrobiana é de fundamental importância na prevenção e no tratamento da mastite infecciosa, mas o uso indiscriminado de antimicrobianos atua como fator determinante para a disseminação da doença. O presente estudo avaliou os perfis de resistência de 57 Staphylococcus spp. isolados de mastite clínica bovina em relação ao uso de betalactâmicos e gentamicina, relacionando características do fenótipo (testes de suscetibilidade in vitro) e genótipo (detecção e expressão de genes que codificam resistência - mecA, mecALGA251, blaZ, femA, femB, e aacA-aphD - usando PCR e RT-PCR, respectivamente). Um ou mais genes que codificam resistência a diferentes antimicrobianos foram detectados em 50 Staphylococcus spp. isolados. Os genes femA e femB foram os mais frequentes (75,4% para ambos). A expressão observada dos genes foi a seguinte: blaZ (60%), femA (39,5%), aacA-aphD (50%), femB (32,6%), mecA (8,3%) e mecALGA251 (0%). Considerando-se a relevância do gênero Staphylococcus para a mastite bovina, este estudo teve como objetivo elucidar aspectos referentes aos perfis genotípico e fenotípico desses microrganismos, a fim de contribuir para o desenvolvimento de estratégias eficazes para o controle da mastite.

ABSTRACT Bovine clinical mastitis caused by Staphylococcus spp. is a serious and widespread disease in the world of dairy farming. Antimicrobial therapy is of fundamental importance in the prevention and treatment of infectious mastitis, but the indiscriminate use of antimicrobials acts as a determining factor for the spread of the disease. The present study evaluated the resistance profiles of 57 Staphylococcus spp. isolated from bovine clinical mastitis to beta-lactams and gentamicin, relating characteristics of phenotype (in vitro susceptibility tests) and genotype (detection and expression of genes encoding resistance - mecA, mecALGA251, blaZ, femA, femB, and aacA-aphD - using PCR and RT-PCR, respectively). One or more genes coding for resistance to different antimicrobials were detected in 50 Staphylococcus spp. isolates. The femA and femB genes were the most frequent (75.4% for both). The observed expression of the genes was as follows: blaZ (60%), femA (39.5%), aacA-aphD (50%), femB (32.6%), mecA (8.3%), and mecALGA251 (0%). Considering the relevance of the genus Staphylococcus to bovine mastitis, this study aimed to elucidate aspects regarding the genotypic and phenotypic profiles of these microorganisms so as to contribute to the development of effective strategies for mastitis control.

Abstract: This paper presents the results of a cross-sectional research that analyzed the relationship between risky dietary behaviors and academic achievement in university students. A survey was applied to students from the University Católica del Norte (Coquimbo-Chile), and measurements were made of their body mass index. The results of the study suggest that students with higher vulnerability to sensitivity to the reward presented higher levels of overeating, or tendency to binge eating. In addition, these students had higher levels of preferences for fatty and sweet foods. These constructs proved to be determinants of a higher body mass index, and a lower academic performance. The results of the study suggest that strategies to promote healthy eating in college students with vulnerability to reward sensitivity, must be more effective in improving overall academic performance.

Resumen Se presentan los resultados de una investigación de tipo transversal, que analizó la relación entre las conductas alimentarias de riesgo y el rendimiento académico en universitarios. Para esto se aplicó encuestas a estudiantes de la Universidad Católica del Norte (Coquimbo-Chile), y se realizaron mediciones de su indice de masa corporal. Los resultados del estudio sugieren que los estudiantes con mayor vulnerabilidad a la sensibilidad ante la recompensa presentaron niveles de sobrealimentación elevados, o tendencia a los atracones de comida. Además, estos estudiantes presentaron mayores niveles de preferencias por alimentos grasos y dulces. Estos constructos resultaron ser determinantes de un elevado índice de masa corporal, y además, de un rendimiento académico más bajo. Los resultados del estudio sugieren que estrategias para promover la alimentación saludable en estudiantes universitarios que presentan vulnerabilidad por sensibilidad ante la recompensa, deben ser más efectivas para mejorar el desempeño académico global.

A mastite subclínica em caprinos acarreta prejuízos econômicos e riscos à saúde pública. Tendo em vista a necessidade de pesquisas que demonstrem as espécies de estafilococos mais isoladas e os testes de sensibilidade que comparem a resistência entre Staphylococcus coagulase negativa (SCN) e positiva (SCP) de cabras com mastite subclínica, os objetivos do presente estudo foram identificar os microrganismos isolados de amostras de leite de cabras com mastite subclínica, bem como definir as espécies de estafilococos e determinar o perfil de sensibilidade de Staphylococcus spp. aos antimicrobianos. Para realizar a coleta das amostras, foram executados os testes da caneca de fundo preto e California mastitis test (CMT) com o leite dos animais reagentes ao CMT. Coletaram-se 226 amostras provenientes de sete rebanhos de caprinos leiteiros, as quais foram encaminhadas para o laboratório, onde foram semeadas para o isolamento do microrganismo e a realização do teste de antibiograma. Dessas amostras, 122 tiveram crescimento bacteriano e as espécies mais isoladas de estafilococos foram: S. epidermidis (24,55%), S. lugdunensis (15,40%) e S. intermedius (13,64%). As amostras apresentaram maior resistência aos antimicrobianos penicilina (81,8%), oxacilina (60,0%) e ampicilina (55,5%). Observou-se maior sensibilidade para enrofloxacina (99,1%), eritromicina (98,2%), gentamicina (98,2%) e vancomicina (98,2%). O S. epidermidis apresentou maior resistência antimicrobiana para a amoxicilina e a penicilina do que o S. lugdunensis e o S. intermedius. Foi verificada uma resistência in vitro semelhante entre os estafilococos coagulase negativa e positiva para a maioria dos antimicrobianos testados. É importante o controle do uso abusivo de antimicrobianos para evitar o surgimento de cepas resistentes.

Subclinical mastitis in goats causes economic losses and risks to public health. Given the need for research that shows the most isolated staphylococci species and sensibility tests comparing the resistance between coagulase-negative (CNS) and positive Staphylococcus (CPS) goats with subclinical mastitis, the aim of this study was to identify the microorganisms isolated from milk samples of goats with subclinical mastitis, as well as define the staphylococci species and determine the sensitivity profile of Staphylococcus spp. to antimicrobials. To collect samples, tests were performed for mug of black background and California mastitis test (CMT), collecting milk from CMT positive animals. A total of 226 samples from seven herds of dairy goats was collected and forwarded to the laboratory, where they were seeded for the isolation of the microorganism and implementing the antibiotic sensibility test. Of these, 122 samples had bacterial growth and the most isolated staphylococci species were: S. epidermidis (24.55%), S. lugdunensis (15.40%) and S. intermedius (13.64%). Samples showed increased resistance to antimicrobials: penicillin (81.8%), oxacillin (60.0%) and ampicillin (55.5%). Greater sensitivity to: enrofloxacin (99.1%), erythromycin (98.2%), gentamicin (98.2%) and vancomycin (98.2%) were observed. The S. epidermidis showed higher antimicrobial resistance to amoxicillin and penicillin than S. lugdunensis and S. intermedius. Similar resistance in vitro between CNS and CPS was observed to most antimicrobials. It is important to control the overuse of antibiotics to prevent the emergence of resistant strains.

The study aimed to determine the chemical composition and ruminal degradability of dry matter (DM) and crude protein (CP) of Erythrina falcata at 105, 120 and 135 days of regrowth. Leaves and petiole samples were dried and 8 g of dry grounded samples were placed in nylon bags in the rumen of three male goats during 0, 24, 48 and 72 hours. The DM and CP values were 24.4 and 21.4% at 105, 24.8 and 23.2% at 120 and, 27.2 and 24.4% at 105 days respectively. The ruminal degradability of potentially degradable insoluble fraction of DM and CP was 29.6 and 42.9% at 105, 24.3 and 31.4% at 120 and, 14.9 and 24.1% at 135 days, respectively, and the effective degradability of DM and CP was 41.3 and 41.2% at 105, 36.5 and 30.3% at 120 and, 32.3 and 30.5% at 135 days respectively. The study showed a higher protein advantage in goats at 105 days of growth of E. falcata foliage.

El estudio tuvo como objetivo determinar la composición química y la degradabilidad ruminal de la materia seca (MS) y proteína cruda (PC) del pisonay (Erythrina falcata) a los 105, 120 y 135 días de rebrote. Se tomaron muestras de hojas y peciolo, se secaron y se colocaron 8 g de muestra molida por bolsa de nylon en el rumen de tres chivatos machos adultos por 0, 24, 48 y 72 horas. Los valores de MS y PC fueron 24.4 y 21.4% a los 105, 24.8 y 23.2% a los 120 y, 27.2 y 24.4% a los 135 días, respectivamente. La degradabilidad ruminal de la fracción insoluble potencialmente degradable de MS y PC fue 29.6 y 42.9% a los 105, 24.3 y 31.4% a los 120 y, 14.9 y 24.1% a los 135 días, respectivamente, y la degradabilidad efectiva de MS y PC fue 41.3 y 41.2% a los 105, 36.5 y 30.3% a los 120 y, 32.3 y 30.5% a los 135 días, respectivamente. El follaje de E. falcata a los 105 días de crecimiento posee una mayor ventaja proteica para los caprinos.