ABSTRACT The plastic film materials are a widely used agricultural means of production. Recently, two types of new agricultural film mulches are developed and are used popularly. They are ethylene-vinyl acetate copolymer (EVA) and polyolefin (PO) film mulches. The transmittances of film mulches are related to thermal reservation and plant photosynthesis. We experimentally measure transmittances of several EVA and PO film mulches under different wavelengths. We also find that the transmittances of mulches vary with light intensity change at one wavelength. The variable transmittances for these mulches are also measured in experiments. These data measured can provides help for agricultural production and accurate numerical simulation for the establishment microclimate of plastic greenhouse, even the degradation of plastic films. Recently popularly ethylenevinyl ethylene vinyl (EVA (PO photosynthesis wavelengths wavelength experiments greenhouse films

Abstract Objectives Nasopharyngeal carcinoma (NPC) is an aggressive epithelial cancer. The expression of miR-186 is decreased in a variety of malignancies and can promote the invasion and metastasis of cancer cells. This study aimed to explore the role and possible mechanism of miR-186 in the metastasis and epithelial-mesenchymal transformation (EMT) of NPC. Methods The expression of miR-186 in NPC tissues and cells was detected by RT-PCR. Then, miR-186 mimic was used to transfect NPC cell lines C666-1 and CNE-2, and cell activity, invasion and migration were detected by CCK8, transwell and scratch assay, respectively. The expression of EMT-related proteins was analyzed by western blotting analysis. The binding relationship between miR-186 and target gene Zinc Finger E-Box Binding Homeobox 1 (ZEB1) was confirmed by double luciferase assay. Results The expression of miR-186 in NPC was significantly decreased, and transfection of miR-186 mimic could significantly inhibit the cell activity, invasion, and migration, and regulate the protein expressions of E-cadherin, N-cadherin and vimentin in C666-1 and CNE-2 cells. Further experiments confirmed that miR-186 could directly target ZEB1 and negatively regulate its expression. In addition, ZEB1 has been confirmed to be highly expressed in NPC, and inhibition of ZEB1 could inhibit the activity, invasion, metastasis and EMT of NPC cells. And co-transfection of miR-186 mimic and si-ZEB1 could further inhibit the proliferation and metastasis of NPC. Conclusion miR-186 may inhibit the proliferation, metastasis and EMT of NPC by targeting ZEB1, and the miR-186/ZEB1 axis plays an important role in NPC. (NPC miR186 miR 186 miR-18 epithelialmesenchymal mesenchymal (EMT RTPCR. RTPCR RT PCR. PCR RT-PCR Then C6661 C C666 C666- CNE2, CNE2 CNE 2, 2 activity CCK8 CCK assay respectively EMTrelated related analysis EBox E Box ZEB (ZEB1 Ecadherin, Ecadherin cadherin, cadherin E-cadherin Ncadherin N CNE- addition cotransfection co siZEB1 siZEB si si-ZEB miR186/ZEB1 miR186ZEB1 miRZEB 186/ZEB1 miR-186/ZEB miR18 18 miR-1 C66 (ZEB miR186/ZEB miR186ZEB 186ZEB1 186/ZEB miR1 miR- C6 186ZEB

SUMMARY OBJECTIVE: The objective of this study was to explore the relationship between serum soluble fms-like tyrosine kinase 1 and the severity of acute pancreatitis and its diagnostic utility. METHODS: This study was carried out by searching Chinese and English literature from the establishment of the database to July 9, 2023, systematically, and assessing the quality and heterogeneity of the articles included. RESULTS: Thirteen studies with a total of 986 patients were included. Patients with severe acute pancreatitis showed higher levels of soluble fms-like tyrosine kinase 1 compared with mild acute pancreatitis [weighted mean difference=76.64 pg/mL, 95% confidence interval (95%CI 50.39–102.89, p<0.001)]. Soluble fms-like tyrosine kinase 1 predicted pooled sensitivity, specificity, and area under the curve were 79%, 74%, and 0.85 for severe acute pancreatitis, with some heterogeneity (I2>50% or p<0.05). In the subgroup analysis, cutoff >150 pg/mL was found to be a heterogeneous factor. CONCLUSION: Soluble fms-like tyrosine kinase 1 is a reliable tool for identifying acute pancreatitis severity, but only as a screening tool. OBJECTIVE fmslike fms like utility METHODS 9 2023 systematically included RESULTS 98 weighted difference7664 difference 76 64 difference=76.6 pgmL pg mL 95 95%CI 95CI CI 503910289 50 39 102 89 50.39–102.89 p<0.001. p0001 p p<0.001 . 0 001 p<0.001)] sensitivity specificity 79 79% 74 74% 085 85 0.8 I2>50% I250 I I2 (I2>50 p<0.05. p005 p<0.05 05 p<0.05) analysis 150 >15 factor CONCLUSION 202 difference766 7 6 difference=76. 50391028 5 3 10 8 50.39–102.8 p000 p<0.00 00 p<0.001) 08 0. I2>50 I25 (I2>5 p00 p<0.0 15 >1 20 difference76 difference=76 5039102 50.39–102. I2>5 (I2> p0 p<0. > 2 difference7 difference=7 503910 50.39–102 I2> (I2 p<0 difference= 50391 50.39–10 (I p< 5039 50.39–1 503 50.39– 50.39 50.3 50.

Resumo Antecedentes O sub-diagnóstico e o subtratamento da enxaqueca são tão difundidos que, portanto, é essencial para diagnosticar os portadores de enxaqueca em ambientes não-clínicos. Uma revisão sistemática dos estudos de validação das ferramentas de diagnóstico da enxaqueca aplicáveis a ambientes não-clínicos pode ajudar os pesquisadores e profissionais nas decisões de seleção de ferramentas. Objetivo Revisar sistematicamente e avaliar criticamente estudos de validação publicados sobre ferramentas de diagnóstico da enxaqueca para uso em ambientes não-clínicos, bem como descrever seu desempenho diagnóstico. Métodos Um grupo de trabalho multidisciplinar seguiu procedimentos transparentes e sistemáticos para colaborar neste trabalho. PubMed, Medline e Web of Science foram pesquisados por estudos até 17 de janeiro de 2022. O QUADAS-2 foi empregado para avaliar a qualidade metodológica, e os limites de qualidade adotados pelo estudo da Global Burden Disease foram usados para responder a questões de sinalização. Resultados De 7.214 artigos identificados, um total de 27 estudos examinando 19 ferramentas foram elegíveis para inclusão. Não houve evidência de alta qualidade para apoiar qualquer ferramenta para o uso de diagnóstico de enxaqueca em ambientes não clínicos. A precisão diagnóstica do ID-Migraine, questionário de dor de cabeça estruturada e questionário HARDSHIP foram apoiados por evidências de qualidade moderada, com sensibilidade e especificidade acima de 70%. Deles, o questionário HARDSHIP foi o mais amplamente validado. As 16 ferramentas restantes forneceram provas de má qualidade para o diagnóstico de enxaqueca em populações não-clínicas. Conclusões Até agora, o questionário HARDSHIP é a escolha ideal para o diagnóstico da enxaqueca em ambientes não-clínicos, com precisão diagnóstica satisfatória apoiada por uma qualidade metodológica moderada. Este trabalho revela o próximo passo crucial, que é a realização de mais estudos de validação de alta qualidade em diversos grupos populacionais não-clínicos. subdiagnóstico sub portanto nãoclínicos. nãoclínicos clínicos nãoclínicos, clínicos, PubMed 1 2022 QUADAS2 QUADAS 2 QUADAS- sinalização 7214 7 214 7.21 identificados inclusão IDMigraine, IDMigraine ID Migraine, Migraine ID-Migraine moderada 70 70% Deles validado nãoclínicas. nãoclínicas clínicas. clínicas não-clínicas agora crucial 202 721 21 7.2 20 72 7.

Abstract Background Migraine underdiagnosis and undertreatment are so widespread, that hence is essential to diagnose migraine sufferers in nonclinical settings. A systematic review of validation studies on migraine diagnostic tools applicable to nonclinical settings can help researchers and practitioners in tool selection decisions. Objective To systematically review and critically assess published validation studies on migraine diagnostic tools for use in nonclinical settings, as well as to describe their diagnostic performance. Methods A multidisciplinary workgroup followed transparent and systematic procedures to collaborate on this work. PubMed, Medline, and Web of Science were searched for studies up to January 17, 2022. The QUADAS-2 was employed to assess methodological quality, and the quality thresholds adopted by the Global Burden Disease study were used to tail signaling questions. Results From 7,214 articles identified, a total of 27 studies examining 19 tools were eligible for inclusion. There has been no high-quality evidence to support any tool for use of migraine diagnosis in nonclinical settings. The diagnostic accuracy of the ID-migraine, structured headache and HARDSHIP questionnaires have been supported by moderate-quality evidence, with sensitivity and specificity above 70%. Of them, the HARDSHIP questionnaire has been the most extensively validated. The remaining 16 tools have provided poor-quality evidence for migraine diagnosis in nonclinical populations. Conclusions Up till now, the HARDSHIP questionnaire is the optimal choice for diagnosing migraine in nonclinical settings, with satisfactory diagnostic accuracy supported by moderate methodological quality. This work reveals the crucial next step, which is further high-quality validation studies in diverse nonclinical population groups. widespread decisions performance PubMed Medline 17 2022 QUADAS2 QUADAS 2 QUADAS- questions 7214 7 214 7,21 identified 1 inclusion highquality high IDmigraine, IDmigraine ID migraine, ID-migraine moderatequality 70 70% them validated poorquality poor populations now step groups 202 721 21 7,2 20 72 7,

Abstract As a natural agricultural product, honey is favored by consumers, and its variety and adulteration have a huge impact on the quality. Acacia honey, red jujube honey and rape honey were used as experimental objects, and their spectral reflectance curves were obtained through a near-infrared spectral image acquisition system. Spectral features were extracted from the preprocessed spectral reflectance curves, and a honey variety classification model based on near-infrared spectral features was established by machine learning. After statistical analysis, Principal Component Analysis Support Vector Machine after processing data through Successive Projections Algorithm (SPA-SVM) is the optimal classification model for three varieties of acacia honey, red jujube honey and rape honey, and the correct rate of honey variety classification reaches 95.83%. The spectral reflectance curve was used to establish a honey adulteration identification model based on the partial least squares-discriiminate analysis (PLS-DA), and the classification accuracy was 97.92% in the test set.

Resumo Fundamento A dexmedetomidina (DEX), um agonista específico do receptor α2-adrenérgico, é protetora contra lesão de isquemia/reperfusão miocárdica (I/R). No entanto, a associação entre a cardioproteção induzida pelo pré-condicionamento DEX e a supressão da mitofagia permanece pouco clara. Objetivo Portanto, nosso objetivo foi investigar se o pré-condicionamento com DEX alivia a I/R, suprimindo a mitofagia via ativação do receptor α2-adrenérgico. Método Sessenta corações de ratos isolados foram tratados com ou sem DEX antes de induzir isquemia e reperfusão; um antagonista do receptor α2-adrenérgico, a ioimbina (YOH), também foi administrado antes da isquemia, isoladamente ou com DEX. A frequência cardíaca (FC), pressão diastólica do ventrículo esquerdo (PDVE), pressão diastólica final do ventrículo esquerdo (PDFVE), taxa máxima e mínima de desenvolvimento da pressão ventricular esquerda (±dp/dtmax) e tamanho do infarto do miocárdio foram medidos. A ultraestrutura mitocondrial e as autofagossomas foram avaliadas por microscopia eletrônica de transmissão. O potencial de membrana mitocondrial e os níveis de espécies reativas de oxigênio (ROS) foram medidos usando os ensaios JC-1 e diacetato de diclorodi hidrofluoresceína, respectivamente. Os níveis de expressão das proteínas associadas à mitofagia Beclin1, relação LC3II/I, p62, PINK1 e Parkin foram detectados por western blotting. Resultados Em comparação com o grupo controle, no grupo isquemia/reperfusão, a FC, PDVE e ±dp/dtmax foram notavelmente diminuídas (p<0,05), enquanto os tamanhos da PDFVE e do infarto aumentaram significativamente (p<0,05). O pré-condicionamento com DEX melhorou significativamente a disfunção cardíaca, reduziu o tamanho do infarto do miocárdio, manteve a integridade estrutural mitocondrial, aumentou o potencial de membrana mitocondrial, inibiu a formação de autofagossomas e diminuiu a produção de ROS e a relação Beclin1, relação LC3II/I, expressão PINK1, Parkin e p62(p<0,05). Quando DEX e YOH foram combinados, o YOH cancelou o efeito da DEX, enquanto o uso de YOH sozinha não teve efeito. Conclusão Portanto, o pré-condicionamento DEX foi cardioprotetor contra I/R em ratos, suprimindo a mitofagia por meio da ativação do receptor α2-adrenérgico. , (DEX) α2adrenérgico, α2adrenérgico αadrenérgico α2 adrenérgico, adrenérgico α α2-adrenérgico isquemiareperfusão reperfusão I/R. IR . I R (I/R) entanto précondicionamento pré condicionamento clara Portanto α2adrenérgico. adrenérgico. YOH, (YOH) FC (FC) PDVE, (PDVE) PDFVE, (PDFVE) dpdtmax dp dtmax (±dp/dtmax transmissão (ROS JC1 JC 1 JC- hidrofluoresceína respectivamente Beclin1 Beclin LC3III LCIII LC3II LC II LC3II/I p62 p PINK blotting controle p<0,05, p005 p<0,05 0 05 (p<0,05) p<0,05. p62p<0,05. p62p005 pp p62(p<0,05) combinados (DEX (I/R (YOH (FC (PDVE (PDFVE III LCII p6 p00 p<0,0 (p<0,05 p62p p62p<0,05 p62p00 p62(p<0,05 p0 p<0, (p<0,0 p62p<0,0 p62p0 p62(p<0,0 p<0 (p<0, p62p<0, p62(p<0, p< (p<0 p62p<0 p62(p<0 (p< p62p< p62(p< (p p62(p

Abstract Background Dexmedetomidine (DEX), a specific α2-adrenergic receptor agonist, is protective against myocardial ischemia/reperfusion injury (MIRI). However, the association between DEX preconditioning-induced cardioprotection and mitophagy suppression remains unclear. Objective Hence, we aimed to investigate whether DEX preconditioning alleviates MIRI by suppressing mitophagy via α2-adrenergic receptor activation. Method Sixty isolated rat hearts were treated with or without DEX before inducing ischemia and reperfusion; an α2-adrenergic receptor antagonist, yohimbine (YOH), was also administered before ischemia, alone or with DEX. The heart rate (HR), left ventricular diastolic pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), maximal and minimal rate of left ventricular pressure development (±dp/dtmax), and myocardial infarction size were measured. The mitochondrial ultrastructure and autophagosomes were assessed using transmission electron microscopy. Mitochondrial membrane potential and reactive oxygen species (ROS) levels were measured using JC-1 and dichloride hydrofluorescein diacetate assays, respectively. The expression levels of the mitophagy-associated proteins Beclin1, LC3II/I ratio, p62, PINK1, and Parkin were detected by western blotting. Results Compared with the control group, in the ischemia/reperfusion group, the HR, LVDP, and ±dp/dtmax were remarkably decreased (p< 0.05), whereas LVEDP and infarct sizes were significantly increased (p< 0.05). DEX preconditioning significantly improved cardiac dysfunction reduced myocardial infarction size, maintained mitochondrial structural integrity, increased mitochondrial membrane potential, inhibited autophagosomes formation, and decreased ROS production and Beclin1, LC3II/I ratio, PINK1, Parkin, and p62 expression(p< 0.05). When DEX and YOH were combined, YOH canceled the effect of DEX, whereas the use of YOH alone had no effect. Conclusion Therefore, DEX preconditioning was cardioprotective against MIRI in rats by suppressing mitophagy via α2-adrenergic receptor activation. , (DEX) α2adrenergic αadrenergic α2 adrenergic α agonist ischemiareperfusion reperfusion MIRI. . (MIRI) However preconditioninginduced induced unclear Hence activation antagonist YOH, (YOH) HR (HR) LVDP (LVDP) enddiastolic end LVEDP, (LVEDP) ±dp/dtmax, dpdtmax dp dtmax (±dp/dtmax) microscopy (ROS JC1 JC 1 JC- assays respectively mitophagyassociated associated Beclin1 Beclin LC3III LCIII LC3II I LC II ratio p PINK1 PINK blotting group p< (p 0.05, 005 0.05 0 05 0.05) 0.05. integrity formation p6 expressionp< expressionp expression(p combined Therefore (DEX (MIRI (YOH (HR (LVDP (LVEDP (±dp/dtmax III LCII 00 0.0 0.

Abstract To investigate the value of edible horticultural therapy (EHT) for long-term-hospitalized (LTH) female patients who suffered from schizophrenia. 60 female schizophrenic patients hospitalized in our hospital were randomly divided into EHT group and control group, with 30 cases in each group. The EHT group was combined with EHT on the basis of drug treatment, and the control group was only treated with drug treatment. The Brief Psychiatric Rating Scale (BPRS), the Chinese version Scale of Social Functioning for Psychotic Inpatients (SSFPI), and the Life Satisfaction Index A (LSIA) were compared and analyzed between two groups. After 6 weeks of treatment, the SSFPI score of EHT group increased and was significantly higher than that of the control group. After treatment, the LSIA index of EHT group was higher than that of the control group, and the difference between the two groups was statistically significant (P < 0.05). The BPRS index of EHT group was significantly lower than that before treatment, but there was little change in the control group. There was significant difference between the two groups (P < 0.05). EHT maybe valuable for improving clinical symptoms of schizophrenia and recovery of social function. Clinical registration number:researchregistry7590

ABSTRACT Soil moisture (SM) plays an important role in regulating the global water cycle, especially in arid areas, and is one of the main indicators of ecological environmental health. Although traditional methods can accurately measure SM at a single sample site, they are limited in large-scale and dynamic SM monitoring. Therefore, we used the Landsat images as the data source and the soil adjusted vegetation index (SAVI) to build the adjusted SAVI (aSAVI) index by modifying the soil adjustment parameter L and introducing the short-wave infrared band. According to the theory of temperature vegetation dryness index (TVDI) and feature space, we introduced a model, combined the measured SM data (Minqin Basin, China) through a comparative analysis of four vegetation indices (NDVI, SAVI, MSAVI, aSAVI) determine the optimal model. Taking the Minqin Basin as the study area, the spatiotemporal variation characteristics of SM in three sub-regions (the entire study area, irrigated region, and periphery of the irrigated regions) were quantitatively analyzed and compared in four different periods: pre-Comprehensive Treatment Program of the Shiyang River Basin (pre-CTSRB) (2000–2005), CTSRB I (2006–2010), CTSRB II (2011–2016), and CTSRB-end (2017–2021) to evaluate the ecological restoration effects of treatment programs from the SM perspective. The results showed that: 1) SM values derived from TVDI inversion and the aSAVI were more accurate, and the model sensitivity decreased with soil depth; 2) the mean value of SM fluctuated across the four periods but decreased slightly over the entire time series. The spatial variations of the SM were characterized by a “descending then ascending” trend. Soil moisture increased in 21.35 % of areas at 0.00-0.10 m in the past 22 years, and 59.66 % at 0.10-0.20 m. There was a negative correlation between the mean variation trend of SM and the percentage of area where SM fell in different periods; 3) the treatment program positively affected the ecological restoration of the Minqin Basin from the SM perspective. The area where SM increased was larger than that of decreasing SM, especially in 0.10-0.20 m soil layer. The increase can promote growth and confer resistance to desertification.

Abstract The classification algorithms of different peanut varieties were studied based on hyperspectral imaging technology. Firstly, the spectral images of five peanut species were collected by hyperspectral instrument produced by Zhuolihanguang Co., LTD. Then SpacVIEW was used to correct the spectral images in black and white, and ENVI5.1 was used to extract the interest in the spectral image of each peanut and calculate the mean spectral reflection value of the region. The spectral characteristic curves of the five peanut samples all showed certain differences, which lays a foundation for the feasibility of modeling in the next step. In order to eliminate the influence of non-quality factor information in hyperspectral spectral data, a variety of data preprocessing methods were used to eliminate noise in the original spectral data, and XGBoost, LightGBM, CatBoost and GBDT algorithms were used to extract feature bands. XGBoost and LightGBM were then used for classification modeling of extracted feature bands. In the classification model, both XGBoost and LightGBM can reach 99.33%, while other performance evaluation indexes cannot distinguish these two models well. Therefore, Optuna algorithm was selected to optimize the two algorithms respectively. After optimization, both LightGBM and XGBoost have improved to varying degrees, but LightGBM is relatively obvious, especially in fit_time, which is 11 times faster than XGBoost and 16 times faster than before optimization. Therefore, the best classification algorithm selected in this study is MF-LightGBM-LightGBM-Optuna-LightGBM. The research of peanut classification method provides a strong theoretical basis and technical support for the revitalization of rural industry, the integration of peanut agriculture and industry and the acceleration of the modernization of agricultural industry system, production system and management system.

Abstract We aimed to establish a method for the rapid identification of three common Gram-positive bacteria (Bacillus cereus, Listeria monocytogenes, and Micrococcus luteus) associated with foodborne diseases. MALDI-TOF-MS was used to determine the effects of sample pretreatment, culture medium, and culture time on the identification results. Then, MALDI-TOF-TOF-MS was used to establish an optimized method and further explore the effects of culture time on secondary proteins. Among the three sample pretreatment methods, formic acid extraction outperformed direct transfer and extended direct transfer, resulting in more protein peaks and higher peak intensity in B. cereus culture samples. However, with different culture times (1–7 d), ion peaks of all bacterial proteins were relatively stable according to MALDI-TOF-TOF-MS data. The protein peaks of B. cereus (1430 m/z), L. monocytogenes (2100 m/z), and M. luteus (2140 m/z) were split into small ion peaks at 1162, 1465, and 1625 m/z, respectively. After 7 d of culture, the secondary spectra, peak intensities, and peak values of formic acid-treated samples were relatively stable, indicating that secondary protein peaks were less affected by culture time. It provides a new approach for the routine identification and market supervision of food safety in China.

Abstract This study aims to investigate the effect and specific mechanism of Coix Seed Oil (CSO) on immune hepatic fibrosis in immune injury rats. In this study, healthy Wister rats were injected with porcine serum in order to establish hepatic fibrosis model. We choose the compound Sophora flavescens injection as the positive control drug. HE staining was used to observe the morphological and histological changes. HSC-T6 cells were cultured in vitro. MTT and AO/EB staining were used to detect the effect of CSO on the proliferation and apoptosis of HSC-T6 cells. The results shown that Coix seed oil significantly improved hepatic fibrosis of immune injury rats, and the degree of hepatic fibrosis in CSO group was significantly reduced. Intraperitoneal injection of 15 mg/kg, 30 mg/kg and 40 mg/kg significantly reduced the levels of serum ALT, AST, ALP, HA, LN, PIIINP and C-IV in rats (P < 0.01).), MTT assay showed that the cells proliferated well. The results of HE staining showed that Coix seed oil had a good therapeutic effect on immune-induced hepatic fibrosis in rats. AO/EB staining showed that 0.5 mg/mL CSO for 24 h and 0.1 mg/mL CSO for 48 h both significantly promoted the apoptosis of HSC-T6 cells. In conclusion, Coix seed oil has a significant therapeutic effect on immune hepatic fibrosis, and its molecular mechanism may be related to antagonizing cytokine TGF-β1, inhibiting the expression of TIMP-1, promoting collagen degradation, inhibiting the activation of hepatocyte and promoting the apoptosis of activated hepatic stellate cells.

Nonalcoholic fatty liver disease (NAFLD) is considered to be a manifestation of hepatic metabolic syndrome. Some studies on the pathogenesis of NAFLD by targeting gut microbiota have attracted wide attention. Previous studies have demonstrated the positive effects of berberine and evodiamine on metabolic diseases and gut microbiota dysbiosis. However, it is not known whether the combination of berberine and evodiamine (BE) can prevent the development of high-fat diet (HFD)-induced NAFLD. Therefore, we aimed to explore the protective effects of BE on the development of HFD-induced NAFLD from the perspective of the gut microbiota. Gut microbiota profiles were established by high throughput sequencing of the bacterial 16S ribosomal RNA gene. The effects of BE on liver and intestinal tissue, intestinal barrier integrity, and hepatic inflammation were also investigated. The results showed that the abundance and diversity of gut microbiota were enriched by BE treatment, with an increase in beneficial bacteria, such as Lactobacillus, Ruminococcus, and Prevotella, and a decrease in pathogenic bacteria such as Fusobacterium and Lachnospira. In addition, BE effectively improved liver fat accumulation and tissue damage, inhibited the apoptosis of intestinal epithelial cells, increased the contents of intestinal tight junction proteins, and decreased the expression of pro-inflammatory factors. Consequently, BE treatment could be an effective and alternative strategy for alleviating NAFLD by modulating gut microbiota and safeguarding the intestinal barrier.

Abstract Prediction of seed vigor based on hyperspectral peant. The traditional method is time-consuming and laborious to detect seed vigor. At the same time, the accuracy of the detection result is not high, and it will cause damage to the seed itself. Therefore, in order to achieve rapid and non-destructive detection of peanut seed vigor, the test was performed with original health, artificial aging for 24h and Peanut seeds with different vigor gradients at 72 hours were used as the research samples. Hyperspectral images with a wavelength range of 387~1035 nm were collected, and the image of the central part of the peanut seeds with a pixel size of 60 × 60 after correction was intercepted and the average reflectance value was calculated. After a combination of processing analysis, characteristic band processing, and model selection, a hyperspectral prediction system with the highest correlation to the viability of extracted peanut seeds was finally established. Experiments shown that the combination of hyperspectral imaging technology and the MF-LightGBM-RF model had the best performance, with a prediction accuracy of 92.59% and a fitting time of 1.77s, which simplifies the model and improves efficiency.

Abstract This study investigated the hyperspectral reflectance response of time series generated during oven drying to changes in the moisture content of potato tubers. Seventeen preprocessing methods were used to eliminate the influence of spectral noise on the spectral characteristic curve. Algorithms such as CatBoost, LightGBM, and XGBoost are used to obtain the first 40 effective characteristic spectra of hyperspectral images, which reduces the redundancy of data and improves the prediction accuracy. The water content prediction model of potato tubers was established by using the selected characteristic bands. The results showed that the combined model based on Lasso and XGBoost algorithm had the strongest prediction ability. The best model is MF-Lasso-XGBoost, which has R2 value of 0.8908, Rmse of 0.0610, Mdae of 0.0389, and R2cv of 0.8448. This research can provide reference for the detection of potato moisture content and theoretical basis for the development of crop moisture detector.

Abstract Genetically modified soybean strains are trade monitoring object by China. Currently, the genetically modified soybean MON89788 has been approved in China to be imported as processing raw material. Therefore, there is an urgent need to establish a method to quantitatively assess MON89788. This study used droplet digital PCR technology to quantitatively detect MON89788. The results showed that the genomic DNA concentration and its copy number of genetically modified soybeans showed a certain linear relationship. The formula was y = 2.5967x + 3.1437, where R2 = 0.999. When the same 4.5% genetically modified sample was contained, the results of the droplet digital PCR ratio and linear methods were 5.88% and 3.599%, respectively. Comparing the two results showed that the droplet digital PCR ratio method has a larger error than the linear relationship method. The novel droplet digital PCR linear relationship method established in this study has high sensitivity and specificity, and thus is a good prospect for quantitative research.