ABSTRACT Objective: This study aimed to identify differentially expressed microRNAs (miRNAs) in exosomes derived from the blood plasma of Rheumatoid Arthritis (RA) patients and explore their clinical significance and biological roles. Methods: Illumina high-throughput sequencing was employed to measure miRNA expression levels in plasma exosomes, followed by validation using qRT-PCR. The correlation between exosomal miRNAs and disease activity was systematically analyzed. Additionally, the pathogenic effects of RA exosomes were investigated through bioinformatics analysis and in vitro experiments. Results: Significantly reduced levels of exosomal miR-144-3p and miR-30b-5p were observed in RA patients, which were negatively correlated with DAS28 scores and anti-CCP antibody levels. ROC curve analysis showed that miR-144-3p and miR-30b-5p in plasma exosomes could effectively distinguish RA patients from healthy controls, with AUC values of 0.725 and 0.773, respectively. Combining bioinformatics analysis and in vitro experiments, it was demonstrated that plasma exosomes contribute to ongoing autoantibody production in RA by promoting B-cell differentiation and antibody production. Conclusion: The present study indicates that plasma exosomes from RA patients may be potentially pathogenic. Exosomal miR-144-3p and miR-30b-5p exhibit significant decreases in RA patients and are associated with disease activity, suggesting their potential as valuable biomarkers for RA. Objective (miRNAs (RA roles Methods highthroughput high throughput qRTPCR. qRTPCR qRT PCR. PCR qRT-PCR analyzed Additionally experiments Results miR1443p miRp miR 144 3p p miR30b5p miRbp 30b 5p b DAS DAS2 antiCCP anti CCP controls 0725 0 725 0.72 0773 773 0.773 respectively Bcell B cell Conclusion 14 072 72 0.7 077 77 0.77 1 07 7 0.

Abstract With the rapid development of China's economy, many long-span bridges have been built and put into service. Vehicle load has been changing year by year in terms of the gross vehicle weight (GVW), the wheelbase and the traffic volume, especially the overload of heavy vehicles, which is a major challenge to the safety and durability of bridges. It is necessary to establish the vehicle load model through the measured traffic data for actual traffic conditions of the given bridge. In this study, the weigh-in-motion (WIM) data collected from an operational long-span urban highway bridge located in Wuhan, China, were used to analyze the statistical characteristics of vehicle loads. On the basis of the types of vehicles, (1) Considering the double or multimodal Gauss distribution characteristics of the GVW, the expectation maximization (EM) algorithm was used to estimate the statistical parameters of the Gauss distribution; (2) The generalized extreme value distribution (GEV) was used to develop the statistical model of the vehicle load extreme value; (3) The vehicle load extreme values in the design reference period of the urban highway bridge were estimated by the extreme value type I distribution; (4) According to the axle weight and the axle spacing, a statistical fatigue vehicle load model for the small and medium span urban highway bridges located in Wuhan, China was presented based on the Miner linear accumulated damage hypothesis and the effective fatigue damage principle.

The long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3), a tumor suppressor, is critical for the carcinogenesis and progression of different cancers, including hepatocellular carcinoma (HCC). To date, the roles of lncRNA MEG3 in HCC are not well illustrated. Therefore, this study used western blot and qRT-PCR to evaluate the expression of MEG3, miR-9-5p, and Sex determining Region Y-related HMG-box 11 (SOX11) in HCC tissues and cell lines. RNA pull-down and luciferase reporter assay were used to evaluate these molecular interactions. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry detected the viability and apoptosis of HCC cells, respectively. The results showed that MEG3 and SOX11 were poorly expressed but miR-9-5p was highly expressed in HCC. The expression levels of these molecules suggested a negative correlation between MEG3 and miR-9-5p and a positive correlation with SOX11, confirmed by Pearson's correlation analysis and biology experiments. Furthermore, MEG3 could combine with miR-9-5p, and SOX11 was a direct target of miR-9-5p. Moreover, MEG3 over-expression promoted cell apoptosis and growth inhibition in HCC cells through sponging miR-9-5p to up-regulate SOX11. Therefore, the interactions among MEG3, miR-9-5p, and SOX11 might offer a novel insight for understanding HCC pathogeny and provide potential diagnostic markers and therapeutic targets for HCC.

ABSTRACT To study what kind of role uric acid play on the relationship between oxidative Stress and inflammation in peripheral and cerebral system of oxonate-induced hyperuricemic rats. Twenty-six eight male Wistar rats were divided into two groups randomly. Potassium oxonate was used to establish hyperuricemic model for four weeks. In 2nd and 4th week, uric acid (UA) level, total superoxide dismutase (T-SOD), Gu,Zn-SOD activity and interleukin-1beta (IL-1β) concentration in serum were determined respectively. In 4th week, one hour after last PO treatment, five rats of every group were given Evans Blue to test blood-brain barrier (BBB) permeability. Other brains were obtained to analysis T-SOD, Gu,Zn-SOD activity and IL-1β concentration in cerebral system. Meanwhile, brain and kidney were stained with hematoxylin and eosin (H&E) to observe pathological change. In 2nd week, both of T-SOD and Gu,Zn-SOD activity in serum increased obviously (P<0.05) in hyperuricemia rats. However, IL-1β content didn’t change remarkably. In the 4th week, T-SOD activity in model group had become similar with control group, and at the same time IL-1β content in serum increased significantly (P<0.05). Pathological section showed the structural and functional unit of the kidney had been damaged. On the contrary, both of T-SOD and Gu,Zn-SOD activity in brain increased obviously (P<0.05), but IL-1β concentration was no significant difference between two groups. In addition, the results of Evans Blue and H&E suggested the integrity of BBB and structure of brain were not changed after PO treatment. The permeability of BBB and form of UA would be potential factors to decide what kind role UA play on keeping balance between anti-oxidative stress and induction of inflammatory response.

A series of MAl2O4:Eu2+,Sm3+ (M = Ca, Sr, Ba) phosphors was prepared by the combustion method, and the influence of these alkaline earth metals on the structure and luminescent performances for these phosphors was investigated. A relationship was established between their composition, crystallization capacity and luminescent properties. The results show that the CaAl2O4: Eu²+,Sm³+ and SrAl2O4:Eu²+,Sm³+ phosphors exhibit monoclinic crystal systems and that the BaAl2O4:Eu²+,Sm³+ phosphor exhibits a hexagonal structure. The alkaline earth metal has a positive effect on the crystallization process, the heat treatment procedure and the luminescent properties of the corresponding phosphors. According to the order Ca > Sr > Ba, the maximum emission wavelengths of the corresponding phosphors are found at 505 nm, 490 nm and 478 nm, and the relevant colours of emitted light are between blue and green, which indicates that there is a clear blue shift in the emission spectra, but their excitation spectra show almost no difference. The decay curve shows that the afterglow intensity and time are not only related to the activating processes and the hole transport, but also to the phase structure and the grain size, which is in the order Sr > Ca > Ba.