It is important to identify the types of tobacco diseases accurately and take effective control measures in time to improve the efficiency of tobacco planting. In this paper, a hand-held near-infrared spectrometer was used to collect the spectral data of different types of tobacco disease samples. The training models were established via convolutional neural network algorithm. Meanwhile, the traditional classification algorithms support vector machine and back propagation neural network were also compared. The results showed that the prediction accuracy of convolutional neural network algorithm was the highest and the overall performance of the model was the best. The rapid detection method based on a hand-held near-infrared spectrometer and convolutional neural network algorithm could identify tobacco leaf disease species efficiently, non-destructively, quickly and accurately, which provided a new technical reference for tobacco leaf disease species detection and identification. planting paper handheld hand held nearinfrared near infrared samples Meanwhile compared best efficiently nondestructively, nondestructively non destructively, destructively non-destructively identification

Abstract Pulmonary mesenchymal stem cells (PMSCs) are considered important in therapeutic and regenerative responses to lung injury. Despite extensive studies on the human, porcine, sheep, mouse and rat, it was found that PMSCs, which can been performed on goose, have not been well explored. Elucidation of cell differentiation, proliferation, which have not yet been well described, may promote the development of injured lung therapy. In our study, we firstly disclosed biological characteristics of spindle-shaped PMSCs from pulmonary tissues of goose embryos. Growth kinetics and counting kit-8 (CCK8) assay were employed for proliferative activity evaluation. When appropriately induced, PMSCs could differentiate into osteoblasts, adipocytes, chondrocytes, type II alveolar epithelial cells (ATII), which demonstrated that PMSCs have cross-embryonic layer differentiation potential. Besides, undifferentiated PMSCs consistently expressed MSCs associated phenotypes, such as CD166, CD90, CD44, CD29, CD71 and CD105, as identified by immunohistochemistry and RT-PCR. Karyotype analysis demonstrated that PMSCs possessed stable diploid karyotype. Collectively, we successfully established applicable methods for isolation, culture, identification and characterization of goose PMSCs which suggested a potential therapeutic use in regenerative therapy. (PMSCs injury human porcine sheep rat explored proliferation described therapy study spindleshaped spindle shaped embryos kit8 kit 8 kit- CCK8 CCK (CCK8 evaluation induced osteoblasts adipocytes chondrocytes ATII, ATII , (ATII) crossembryonic cross embryonic Besides phenotypes CD166 CD CD90 CD44 CD29 CD7 CD105 RTPCR. RTPCR RT PCR. PCR RT-PCR karyotype Collectively isolation culture (CCK (ATII CD16 CD9 CD4 CD2 CD10 CD1

Previous studies show that glycogen synthase kinase 3β (GSK3B) plays an important role in tumorigenesis. However, its role in cervical cancer is unclear. The present study silenced GSK3B with siRNAs and/or chemical inhibitors to determine its role in HeLa cervical cancer cell proliferation and migration as well as in xenograft tumor growth. Cell Counting Kit (CCK)-8 and 5-ethynyl-2'-deoxyuridine (EdU) assays were used to determine cell survival and proliferation. Scratch and Transwell® assays were used to evaluate cell migration. Xenograft tumors were used to evaluate the effect of GSK3B on tumor growth. Transcriptomic sequencing was used to clarify the mechanisms underlying the foregoing processes. Public databases and clinical specimens showed that GSK3B was upregulated in cervical cancer tissues and correlated with poor prognosis. In vitro experiments indicated that GSK3B inhibition reduced cell viability, proliferation, and migration. In vivo experiments demonstrated that GSK3B inhibition slowed xenograft tumor growth. Transcriptomic sequencing revealed that GSK3B inhibition modulated the phosphatidylinositol 3-carboxykinase (PI3K)/protein kinase B (Akt) and extracellular matrix (ECM)-receptor interaction signaling pathways. GSK3B inhibition decreased the protein levels of phosphorylated PI3K and Akt and the levels of mesenchymal markers but increased those of epithelial markers. An activator of the PI3K/Akt signaling pathway counteracted the suppressive effects of GSK3B inhibition on HeLa cell viability and proliferation and on PI3K/Akt signaling. Our data suggested that GSK3B regulated cervical cancer cell proliferation and migration by modulating the PI3K/Akt signaling pathway and epithelial-to-mesenchymal transition (EMT). β GSKB GSK (GSK3B tumorigenesis However unclear andor or growth CCK8 CCK 8 (CCK)- 5ethynyl2deoxyuridine ethynyldeoxyuridine 5 ethynyl 2 deoxyuridine EdU (EdU Transwell processes prognosis 3carboxykinase carboxykinase 3 PI3K/protein PI3Kprotein PIKprotein /protein PI K (Akt ECMreceptor ECM receptor pathways PIK PI3KAkt PIKAkt epithelialtomesenchymal EMT. EMT . (EMT) (CCK) Kprotein KAkt (EMT (CCK

Abstract Objectives Research has demonstrated that obesity may be associated with rheumatoid arthritis (RA). In addition, gut microbiota and its metabolites contribute to the occurrence and development of RA and obesity. However, the mechanism by which obesity affects RA remains unclear. In this study, we aimed to investigate whether gut microbiota and their metabolites alter the effects of high fat diet (HFD) on the severity of collagen-induced arthritis (CIA) in mice. Methods Briefly, mice were divided into normal group (N), CIA model group (C), HFD group (T), and HFD CIA group (CT). Hematoxylin and Eosin staining(HE) and Safranin O-fast green staining were conducted, and levels of blood lipid and inflammatory cytokines were measured. 16S rDNA sequencing technique and liquid chromatography-mass spectrometry (LC-MS)-based metabolomics were performed to explore changes in the microbiota structure to further reveal the pathomechanism of HFD on CIA. Results HFD aggravated the severity of CIA in mice. The CT group had the highest proportion of microbial abundance ofBlautia, Oscillibacter, Ruminiclostridium-9, andLachnospiraceaeUCG 006 at the genus level, but had a lower proportion of Alistipes. Additionally, the fecal metabolic phenotype of the combined CT group shows significant changes, with differential metabolites enriched in 9 metabolic pathways, including primary bile acid biosynthesis, arginine biosynthesis, sphingolipid metabolism, purine metabolism, linoleic acid metabolism, oxytocin signaling pathway, aminoacyl-tRNA biosynthesis, the pentose phosphate pathway, and sphingolipid signaling pathway. Correlation analysis revealed that some of the altered gut microbiota genera were strongly correlated with changes in fecal metabolites, total cholesterol (TC), triglyceride (TG), and inflammatory cytokine levels. Conclusions This study shows that HFD may aggravate inflammatory reaction in CIA mice by altering the gut microbiota and metabolic pathways. RA. . (RA) addition However unclear (HFD collageninduced collagen induced (CIA Briefly N, N , (N) C, C (C) T, T (T) CT. (CT) stainingHE HE staining(HE Ofast O fast conducted measured S chromatographymass chromatography mass LCMSbased LC MS based ofBlautia Oscillibacter Ruminiclostridium9, Ruminiclostridium9 Ruminiclostridium 9, Ruminiclostridium-9 00 level Alistipes Additionally pathways biosynthesis metabolism pathway aminoacyltRNA aminoacyl tRNA TC, TC (TC) TG, TG (TG) (RA (N (C (T (CT Ruminiclostridium- 0 (TC (TG

ABSTRACT In soybeans from different years, the infrared spectroscopy waveforms exhibit similarities, yet variations in aging time lead to significant differences in their fatty acid content. To rapidly discern the year of soybean production, this study employed ten feature extraction algorithms in conjunction with Convolutional Neural Networks (CNN) to establish models for classifying soybeans from different years (2019, 2020, and 2021). The research findings reveal the following: (1) The CNN models, after feature extraction, all demonstrated improved accuracy. Notably, the optimal models for both powdered and granulated states were the Kpca-CNN models, achieving an accuracy of 100%. The corresponding loss function values were 0.0002 and 0.0007, with processing times of 0.19 seconds and 0.20 seconds, respectively. (2) The modeling results of all models suggest that the classification accuracy of soybean powder spectral data is higher compared to soybean particle spectral data. (3) Validation of the optimal Kpca-CNN model confirmed its consistent accuracy of 100% when introducing new data or reducing the size of the training dataset. In conclusion, the fusion of near-infrared spectroscopy analysis and CNN technology is considered an effective method for classifying soybeans from different years. This method provides a practical solution for the rapid and precise determination of seed production years. similarities content (CNN 2019, 2019 (2019 2020 2021. 2021 . 2021) following 1 (1 Notably KpcaCNN Kpca 100 00002 0 0002 0.000 00007 0007 0.0007 019 19 0.1 020 20 0.2 respectively 2 (2 3 (3 dataset conclusion nearinfrared near 201 (201 202 ( 10 0000 000 0.00 01 0. 02 (20 00 0.0

Abstract Introduction: Propofol is a widely used anesthetic and its dose is closely related to aging. Telomere length (TL) is a unique heritable trait, and emerging as a biomarker of aging, health and disease. Telomerase RNA component (TERC) plays an important role in maintaining TL. We proposed a hypothesis that propofol dose in general anesthesia can be predicted by measuring TL before operation, which greatly reduced the risk of anesthesia, especially the elderly. Methods: The association between the propofol dose in anesthesia induction and: TL in the DNA of peripheral blood leukocytes; body weight; sex; difference of the Bispectral Index (BIS) before and after anesthesia induction in patients was evaluated by multivariable linear regression analyses. The mutation at the 5’end or 3’end of TERC was detected. We recruited 100 patients of elective surgery. Results: We found that propofol dose in anesthesia induction was clearly correlated significantly with TL (r = 0.78, p < 0.001), body weight (r = 0.84, p = 0.004), sex (r = 0.83, p= 0.84, p = 0.004), sex (r = 0.83, p = 0.004), and difference of BIS before and after anesthesia induction (r = 0.85, p = 0.029). By comparing the absolute values of standardized regression coefficients (0.58, 0.21, 0.19, and 0.12) of the four variables, it can be seen that TL contributes the most to the propofol dose in anesthesia induction. However, the mutation at the 5’ end or 3’ end of TERC was not found. Conclusions: These findings provide preliminary evidence that the propofol dose in anesthesia induction was clearly correlated with genetically determined TL. TL may be a promising predictor of the propofol dose, which is beneficial to improve the safety of anesthesia and reduce perioperative complications. Introduction aging (TL trait disease (TERC operation elderly Methods leukocytes (BIS analyses 5end 5 3end 3 detected 10 surgery Results r 078 0 78 0.78 0.001, 0001 0.001 , 001 0.001) 084 84 0.84 0.004, 0004 0.004 004 0.004) 083 83 0.83 085 85 0.85 0.029. 0029 0.029 . 029 0.029) 0.58, 058 58 (0.58 021 21 0.21 019 19 0.19 0.12 012 12 variables However Conclusions complications 1 07 7 0.7 000 0.00 00 08 8 0.8 002 0.02 02 0.58 05 (0.5 2 0.2 01 0.1 0. 0.0 0.5 (0. (0 (

Abstract Background: Prurigo nodularis (PN) is a chronic inflammatory skin condition that has a significant unmet needs for effective treatment options. Objective: To assess the efficacy and safety of dupilumab in adult patients with PN refractory to traditional therapies. Methods: This ongoing, real-life study examined dupilumab treatment in 8 adult patients diagnosed with PN for at least 6 months. The included patients were prescribed 300 mg dupilumab biweekly for at least 16 weeks. Efficacy was the primary outcome by means of multiple standardized scale measurements while safety was also reported. Results: PN patients treated with dupilumab showed notable clinical improvement. After 16 weeks of dupilumab treatment, the mean Investigator Global Assessment (IGA) score reduced from 3.75 to 1.50. Patients mean Numerical Rating Scale Itch Intensity (NRSI), Dermatology Life Quality Index (DLQI), and Hospital Anxiety and Depression Scale (HADS) ratings reduced from 8.625, 15.13, and 14.50 to 1.563, 4.625, and 1.000 respectively. The majority of the patients (87.5%) reported dupilumab as effective while no adverse events have been reported. Study limitations: This study was limited to a small cohort of adult PN patients and a short-time observation period. Conclusions: Dupilumab is effective and tolerable in adult PN patients with significant improvement in skin lesions, itching, and quality of life. Background (PN options Objective therapies Methods ongoing reallife real life months 30 1 Results IGA (IGA 375 3 75 3.7 150 50 1.50 NRSI, NRSI , (NRSI) DLQI, DLQI (DLQI) HADS (HADS 8625 625 8.625 1513 15 13 15.13 1450 14 14.5 1563 563 1.563 4625 4 4.625 1000 000 1.00 respectively 87.5% 875 87 5 (87.5% limitations shorttime short time period Conclusions lesions itching 37 7 3. 1.5 (NRSI (DLQI 862 62 8.62 151 15.1 145 14. 156 56 1.56 462 4.62 100 00 1.0 87.5 (87.5 1. 86 8.6 15. 46 4.6 10 0 87. (87. 8. 4. (87 (8 (

Abstract Objective Our study aimed to elucidate the effect of PAI-1 (Plasminogen Activator Inhibitor-1) and t-PA (Tissue-type Plasminogen Activator) in tissue remodeling in nasal polyps patients. Methods Samples were streamed as early Nasal Polyps (eNP, n = 10) and inferior tissue from the same patient, mature Nasal Polyps (mNP, n = 14), and Control group (n = 15), respectively. Immunohistochemistry and immunofluorescence were applied to detect localization. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blot were used to measure different levels among three groups. The mNP tissue was cultured in vitro and treated with TGF-β1 (Transforming Growth Factor-beta 1) activator, TGF-β1 inhibitor (SB431542), and PAI-1 inhibitor (TM5275); then Western blot, qRT-PCR, and ELISA were used to assess changes. Results The immunohistochemistry and immunofluorescence showed that PAI-1 expression decreased in eNP and mNP, mainly in epithelium and glands. The transcriptional expression and protein level of TGF-β1/t-PA/PAI-1/Collagen1 were lower in eNP than IT while mNP group demonstrated lower mRNA expression and protein level of TGF-β1/t-PA/PAI-1/Collagen1 than Control group. In mNP tissue culture in vitro, TGF-β1 activator elevated t-PA, PAI-1, and Collagen1 with higher release of PAI-1 and Collagen1 in supernatant, whereas SB431542 suppressed above reactions; TM5275 lowered transcriptional and protein level of Collagen1 in supernatant. Conclusion Early Nasal polyps’ formation in middle meatus mucous is related with fibrillation system PAI-1/t-PA and tissue remodeling; moreover, nasal polyps’ development is regulated by TGF-β1-mediated PAI-1 reduction. Level of evidence 3b. PAI1 PAI 1 PAI- Inhibitor1 Inhibitor Inhibitor-1 tPA t PA Tissuetype Tissue type patients eNP, (eNP 10 patient (mNP 14, 14 , 14) 15, 15 15) respectively localization RealTime Real Time qRTPCR qRT PCR (qRT-PCR groups TGFβ1 TGFβ TGF β1 β TGF-β Transforming Factorbeta Factor beta SB431542, SB (SB431542) TM (TM5275) qRTPCR, PCR, qRT-PCR changes glands TGFβ1/tPA/PAI1/Collagen1 TGFβ1tPAPAI1Collagen1 TGFβtPAPAICollagen β1/t PA/PAI 1/Collagen1 Collagen TGF-β1/t-PA/PAI-1/Collagen tPA, PA, PAI1, 1, supernatant SB43154 reactions TM527 PAI1/tPA PAI1tPA PAItPA 1/t moreover TGFβ1mediated TGFβmediated mediated reduction 3b b Inhibitor- (SB431542 (TM5275 TGFβ1/tPA/PAI1/Collagen tPAPAI TGFβ1tPAPAI1Collagen β1t βt PAPAI 1Collagen1 1/Collagen SB4315 TM52 1t (SB43154 (TM527 1Collagen SB431 TM5 (SB4315 (TM52 SB43 (SB431 (TM5 SB4 (SB43 (TM (SB4 (SB

Abstract Objective: To investigate the optimal timing of initial intravenous immunoglobulin (IVIG) treatment in Kawasaki disease (KD) patients. Methods: KD patients were classified as the early group (day 1-4), conventional group (day 5-7), conventional group (day 8-10), and late group (after day 10). Differences among the groups were analyzed by ANOVA and Chi-square analysis. Predictors of IVIG resistance and the optimal cut-off value were determined by multiple logistic regression analyses and receiver operating characteristic (ROC) curve analysis. Results: There were no significant differences in IVIG resistance among the 4 groups (p = 0.335). The sensitivity analysis also confirmed no difference in the IVIG resistance between those who started the initial IVIG ≤ day 7 of illness and those who received IVIG >day 7 of illness (p = 0.761). In addition, patients who received IVIG administration more than 7 days from the onset had a higher proportion of coronary artery abnormalities (p = 0.034) and longer length of hospitalization (p = 0.033) than those who started IVIG administration less than 7 days. The optimal cut-off value of initial IVIG administration time for predicting IVIG resistance was >7 days, with a sensitivity of 75.25% and specificity of 82.41%. Conclusions: IVIG therapy within 7 days of illness is found to be more effective for reducing the risk of coronary artery abnormalities than those who received IVIG >day 7 of illness. IVIG treatment within the 7 days of illness seems to be the optimal therapeutic window of IVIG. However, further prospective studies with long-term follow-up are required. Objective (IVIG (KD Methods 14, 14 1 , 1-4) 57, 57 5 5-7) 810, 810 8 10 8-10) after 10. . 10) Chisquare Chi square cutoff cut off ROC (ROC Results p 0.335. 0335 0.335 0 335 0.335) 0.761. 0761 0.761 761 0.761) addition 0.034 0034 034 0.033 0033 033 > 7525 75 25 75.25 8241 82 41 82.41% Conclusions However longterm long term followup follow up required 1-4 5-7 81 8-10 0.33 33 076 0.76 76 0.03 003 03 752 2 75.2 824 82.41 1- 5- 8-1 0.3 3 07 0.7 0.0 00 75. 82.4 8- 0. 82.

Resumen Objetivo. Investigar la seguridad y la eficacia de la discectomía endoscópica percutánea (DEP) y de la discectomía fenestrada (DF) en el tratamiento de la hernia de disco lumbar. Método. Se analizaron retrospectivamente los datos clínicos completos de 87 pacientes con hernia de disco lumbar. De acuerdo con el tratamiento prescrito, los pacientes fueron divididos en grupo control (DF, n = 39) y grupo de estudio (DEP, n = 48). Se compararon las condiciones básicas de funcionamiento de los dos grupos y se evaluaron los resultados de la cirugía, la incidencia de complicaciones y la calidad de vida al año de la operación. Resultados. Ambos grupos completaron la operación. En el grupo de estudio, las puntuaciones en la escala visual análoga y ODI disminuyeron significativamente, mientras que las puntuaciones JOA aumentaron significativamente. La tasa de éxito de la operación en el grupo de estudio fue significativamente mayor que en el grupo control, y la incidencia de complicaciones fue significativamente menor que en el grupo control. No hubo diferencia significativa en la calidad de vida entre los dos grupos (p > 0.05). Conclusiones. La tasa de éxito del tratamiento con DEP fue mayor, y el tiempo de recuperación fue más rápido y más seguro que con la DF.

Abstract Background. The aim of the study was to explore the safety and efficacy of percutaneous transforaminal endoscopic discectomy (PTED) and fenestration discectomy (FD) in the treatment of lumbar disc herniation (LDH). Material and methods. The complete clinical data from 87 patients with LDH from our hospital were retrospectively analyzed. Patients were divided into a control group (n = 39, treated with FD) and a research group (n = 48, treated with PTED) according to the prescribed treatments. The basic operation conditions were compared across the two groups. Surgical outcomes were assessed. The incidences of complications and the life quality of patients were evaluated 1 year after surgery. Results. The patients in both groups completed the operation. The visual analog scale and Oswestry Disability Index score of patients in the research group was significantly lower while the Orthopaedic Association Score was significantly higher after surgery. The success rate of the operation in the research group which was significantly higher and the rate of complications was significantly lower. No statistical differences in the quality of life were observed between the patients (p > 0.05). Conclusions. PTED and FD are effective in the treatment of LDH. However, our study showed that PTED has a higher rate of treatment success, faster recovery times and is safer than FD.

RESUMO Introdução O salto em distância horizontal consiste em quatro partes: corrida de aproximação, impulsão, voo e queda. Objetivo Estudar a influência da ginástica no salto em distância horizontal. Métodos Oito jardins de infância em uma cidade foram selecionados e divididos em um grupo experimental e um grupo de controle. As crianças do grupo experimental atuaram nos módulos de ginástica e de condicionamento físico. Cada módulo foi submetido a um ciclo contínuo de atividades ao ar livre de manhã e à tarde de atividades infantis, de 1º de junho de 2021 a 31 de março de 2010, por um total de 9 meses. Resultados Durante o período de intervenção, não houve diferença significativa entre o grupo experimental e o grupo controle (P > 0,05), mas houve diferença significativa entre o grupo experimental e as meninas antes e depois da intervenção experimental (P < 0,05), enquanto a diferença entre os meninos não foi estatisticamente significativa, indicando que a amplitude do movimento do tornozelo das meninas foi ligeiramente mais afetada do que a dos meninos durante a intervenção de condicionamento físico. Conclusão Com o aumento da idade e do tempo de treinamento, a faixa de variação do ângulo de articulação das crianças mais velhas no grupo experimental foi significativamente maior do que a do grupo de controle. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction The standing long jump consists of four parts: pre-swing, take-off, flying, and landing. Objective Analyze the influence of gymnastics game fitness on standing long jump. Methods Eight kindergartens in a city were selected and divided into experimental and control groups. The children in the experimental group were intervened in gymnastics and game fitness modules. Each module was intervened in a rolling cycle in the morning and afternoon outdoor activities of children from June 1, 2021, to March 31, 2010, for nine months. Results During the intervention period, there was no significant difference between the experimental group and the control group (P > 0.05), but there was a significant difference between the experimental group and the girls before and after the experimental intervention (P < 0.05), while the difference between boys was not statistically significant, indicating that the range of ankle movement of girls was slightly more affected than boys during the fitness intervention. Conclusion With the increase in age and training time, the change range of joint angles of older children in the experimental group is significantly higher than that in the control group. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Introducción El salto de longitud horizontal consta de cuatro partes: carrera de aproximación, impulsión, vuelo y caída. Objetivo Analizar la influencia de la gimnasia en el salto de longitud de pie. Métodos Se seleccionaron ocho guarderías de una ciudad y se dividieron en un grupo experimental y otro de control. Los niños del grupo experimental realizaron módulos de gimnasia y fitness. Cada módulo intervino en un ciclo continuo de actividades al aire libre por la mañana y por la tarde desde el 1 de junio de 2021 hasta el 31 de marzo de 2010, durante nueve meses. Resultados Durante el período de intervención, no hubo diferencias significativas entre el grupo experimental y el grupo de control (P > 0,05), pero sí entre el grupo experimental y las chicas antes y después de la intervención experimental (P < 0,05), mientras que la diferencia entre los chicos no fue estadísticamente significativa, lo que indica que la amplitud de movimiento del tobillo de las chicas se vio ligeramente más afectada que la de los chicos durante la intervención de acondicionamiento físico. Conclusión Al aumentar la edad y el tiempo de entrenamiento, el rango de variación de los ángulos articulares de los niños mayores del grupo experimental es significativamente mayor que el del grupo de control. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Abstract Compared to other orthodontic acceleration methods such as drug, electric current, and laser, vibratory loading is less invasive and easier to use. But the optimal duration for vibratory load application has not been determined, nor can the alveolar bone parameters be predicted after vibratory load application. Therefore, this work examined the mechanism of vibration-accelerated alveolar bone reconstruction and established a numerical model for simulating alveolar bone damage caused by vibration loads. That is, the role of vibration load in orthodontic acceleration was analyzed, and a finite element model was established to validate the vibra-tion-accelerated orthodontic mechanism with a simulated numerical model of alveolar bone damage. The optimal duration of application was obtained for the right anterior incisor under vibratory loading of 5 N orthodontic force, 50 Hz in the gingival orientation and 0.2 cm amplitude for 120 to 140 minutes. This work is of guidance and reference significance in promoting the development of orthodontic treatment and shortening the orthodontic treatment cycle. drug current laser use determined Therefore vibrationaccelerated accelerated loads analyzed vibra tion force 02 0 2 0. 12 14 minutes cycle 1

Abstract Objectives: Abdominal Aortic Aneurysm (AAA) is a complex disease with both genetic and environmental risk factors. This study aimed to examine the potential association of the +276G/T and −420C>G polymorphisms in the resistin gene with AAA susceptibility and progression. Method: We performed a retrospective study involving AAA patients and healthy controls, assessing the distribution of the +276G/T and −420C>G genotypes in both groups. Hardy-Weinberg equilibrium was assessed for both polymorphisms. Logistic regression was used to explore the influence of these genotypes on AAA occurrence and progression, adjusting for relevant confounders. Results: The distribution of +276G/T polymorphism did not significantly differ between AAA patients and controls. Conversely, a significant difference was observed in the genotype distribution of −420C>G polymorphism between the two groups. The CC genotype and CC/CG genotypes of −420C>G polymorphism were found to be associated with an increased risk and progression of AAA. Conclusions: The −420C>G polymorphism, particularly the CC genotype and CC/CG genotypes, might play a substantial role in AAA susceptibility and progression. The present findings underscore the need for further investigations to confirm these associations and fully elucidate the role of the resistin gene in AAA. Objectives (AAA factors 276GT GT 276G T G 420CG CG 420C C Method controls groups HardyWeinberg Hardy Weinberg confounders Results Conversely CCCG Conclusions

Abstract Objective Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been fully elucidated. To address this, we have investigated the probable anti-inflammatory mechanism of W54011 in LPS-treated inflammation in human gingival fibroblasts (HGFs). Methodology HGFs were isolated from healthy gingival tissue samples using the tissue block method and were identified with immunofluorescence staining. The CCK8 assay and reverse transcription-PCR (RT-PCR) were used to select the optimal induction conditions for Lipopolysaccharide (LPS) and C5aRA (according to supplementary data S1, S2 and S3). The levels of inflammatory cytokines, C5aR, and the activation of NF-κB/MAPK signaling pathways were determined by RT-quantitative PCR (RT-qPCR) and Western blotting. Results Immunofluorescence results showed that vimentin and FSP-1 were positive in HGFs and Keratin was negative in HGFs. Immunofluorescence staining demonstrated that C5aRA inhibited LPS-stimulated nuclear translocation of p-p65. RT-qPCR and Western blotting showed that C5aRA reduced the expression of IL-1β, IL-6, TNF-α, C5aR, p-p65, p-IκBα, p-JNK, p-c-JUN, and TLR4 in LPS-induced HGFs. Conclusion These findings suggested that C5aRA attenuated the release of inflammatory cytokines in LPS-induced HGFs by blocking the activation of the NF-κB and MAPK signaling pathways. W W5401 nonpeptide non peptide CaR C aR CaRA aRA (C5aRA antiinflammatory anti models However elucidated this LPStreated LPS treated . (HGFs) CCK transcriptionPCR transcription RTPCR RT (RT-PCR (LPS according S1 S S3. S3 S3) NFκB/MAPK NFκBMAPK NF κB/MAPK κB RTquantitative quantitative RTqPCR qPCR (RT-qPCR FSP1 FSP 1 FSP- LPSstimulated stimulated pp65. pp65 pp p p65. p65 p-p65 IL1β, IL1β ILβ IL 1β, 1β β IL-1β IL6, IL6 6, 6 IL-6 TNFα, TNFα TNF α, α TNF-α pp65, p65, pIκBα, pIκBα IκBα, IκBα p-IκBα pJNK, pJNK JNK, JNK p-JNK pcJUN, pcJUN c JUN, JUN p-c-JUN TLR LPSinduced induced NFκB W540 (HGFs κBMAPK pp6 p6 p-p6 IL- W54 p-p W5

RESUMEN Introducción Con el desarrollo económico y el progreso tecnológico, se prestó más atención a la educación intelectual en detrimento del acondicionamiento físico, fenómeno que corroboró elevar la obesidad a una preocupación contemporánea en la salud pública. En este sentido, los estudios que pueden servir de referencia para orientar el comportamiento científico de intervención deportiva de los estudiantes universitarios son el centro de la investigación actual. Objetivo Explorar el efecto de diferentes dosis de ejercicio aeróbico sobre la función microcirculatoria en estudiantes universitarios sedentarios. Métodos Se reclutaron 69 estudiantes de la Universidad de Minzu y se dividieron aleatoriamente en los grupos de ejercicio A, B y control. El grupo de ejercicio recibió 12 semanas de intervención de ejercicio aeróbico. Entre ellos, el grupo A practicaba 1-2 veces por semana, el grupo B practicaba ≥ 3 veces por semana. El grupo de control no practicó ningún deporte. Resultados Tras las pruebas, la reactividad microvascular mostró una interacción con el grupo y el tiempo (p<0,01). Entre ellos, el grupo de ejercicio B fue superior al grupo de control y al grupo de ejercicio A (p<0,01), sin diferencias significativas entre el grupo de ejercicio A y el grupo de control (p>0,05). Conclusión El ejercicio aeróbico afecta a la respuesta microvascular corporal y al nivel de endógeno NO. El ejercicio aeróbico de 12 semanas de duración con una frecuencia igual o superior a tres veces por semana puede mejorar la respuesta microvascular de los estudiantes universitarios sedentarios al promover la producción endógena de NO. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. tecnológico físico pública sentido actual 6 1 ellos 2 1- deporte pruebas p<0,01. p001 p p<0,01 . 0 01 (p<0,01) p<0,01, , p>0,05. p005 p>0,05 05 (p>0,05) NO II tratamiento p00 p<0,0 (p<0,01 p>0,0 (p>0,05 p0 p<0, (p<0,0 p>0, (p>0,0 p<0 (p<0, p>0 (p>0, p< (p<0 p> (p>0 (p< (p> (p

RESUMO Introdução Com o desenvolvimento econômico e o progresso tecnológico, mais atenção foi dada à educação intelectual em detrimento do condicionamento físico, fenômeno que corroborou para elevar a obesidade a uma preocupação contemporânea na saúde pública. Nesse sentido, estudos que possam fornecer referência para orientar o comportamento de intervenção esportiva científica de estudantes universitários são o foco da pesquisa atual. Objetivo Explorar o efeito de diferentes doses de exercício aeróbico sobre a função da microcirculação em estudantes universitários sedentários. Métodos Foram recrutados 69 estudantes da Universidade de Minzu, divididos aleatoriamente em grupos de exercícios A, B e controle. O grupo de exercícios recebeu 12 semanas de intervenção de exercícios aeróbicos. Entre eles, o grupo A praticou 1-2 vezes por semana, o grupo B praticou ≥ 3 vezes por semana. O grupo controle não praticou nenhum esporte. Resultados após o teste, a reatividade microvascular mostrou uma interação ao grupo e ao tempo (p<0,01). Entre eles, o grupo de exercícios B foi superior que o grupo controle e o grupo de exercícios A (p<0,01), sem diferenças significativas entre o grupo de exercícios A e o grupo controle (p>0,05). Conclusão O exercício aeróbico afeta a resposta microvascular corporal e o nível endógeno de NO. O exercício aeróbico com duração de 12 semanas com frequência igual ou superior de três vezes por semana pode melhorar a resposta microvascular de estudantes universitários sedentários, promovendo a produção de endógenos NO. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. tecnológico físico pública sentido atual sedentários 6 Minzu 1 aeróbicos eles 2 1- esporte teste p<0,01. p001 p p<0,01 . 0 01 (p<0,01) p<0,01, , p>0,05. p005 p>0,05 05 (p>0,05) NO II tratamento p00 p<0,0 (p<0,01 p>0,0 (p>0,05 p0 p<0, (p<0,0 p>0, (p>0,0 p<0 (p<0, p>0 (p>0, p< (p<0 p> (p>0 (p< (p> (p

ABSTRACT Introduction Due to economic development and technological progress, more attention has been given to intellectual education in detriment of physical conditioning, a phenomenon that has corroborated to raise obesity to a contemporary concern in public health. In this sense, studies that can provide reference to guide the scientific sport intervention behavior of college students are the focus of the current research. Objective Explore the effect of different doses of aerobic exercise on microcirculation function in sedentary college students. Methods 69 students from Minzu University were recruited and randomly divided into exercise groups A, B and control. The exercise group received 12 weeks of aerobic exercise intervention. Among them, group A practiced 1-2 times per week, group B practiced ≥ 3 times per week. The control group did not practice any sport. Results After testing, microvascular reactivity showed an interaction to group and time (p<0.01). Among them, exercise group B was higher than control group and exercise group A (p<0.01), with no significant differences between exercise group A and control group (p>0.05). Conclusion Aerobic exercise affects body microvascular response and endogenous NO level. Aerobic exercise lasting 12 weeks with frequency equal to or greater than three times per week can improve the microvascular response of sedentary college students by promoting endogenous NO production. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. progress conditioning health sense research 6 1 them 2 1- testing p<0.01. p001 p p<0.01 . 0 01 (p<0.01) p<0.01, , p>0.05. p005 p>0.05 05 (p>0.05) level production II outcomes p00 p<0.0 (p<0.01 p>0.0 (p>0.05 p0 p<0. (p<0.0 p>0. (p>0.0 p<0 (p<0. p>0 (p>0. p< (p<0 p> (p>0 (p< (p> (p