Abstract Background As a common disabling disease, irreversible neuronal death due to spinal cord injury (SCI) is the root cause of functional impairment; however, the capacity for neuronal regeneration in the developing spinal cord tissue is limited. Therefore, there is an urgent need to investigate how defective neurons can be replenished and functionally integrated by neural regeneration; the reprogramming of intrinsic cells into functional neurons may represent an ideal solution. Methods A mouse model of transection SCI was prepared by forceps clamping, and an adeno-associated virus (AAV) carrying the transcription factors NeuroD1 and Neurogenin-2(Ngn2) was injected in situ into the spinal cord to specifically overexpress these transcription factors in astrocytes close to the injury site. 5-bromo-2´-deoxyuridine (BrdU) was subsequently injected intraperitoneally to continuously track cell regeneration, neuroblasts and immature neurons marker expression, neuronal regeneration, and glial scar regeneration. In addition, immunoprotein blotting was used to measure the levels of transforming growth factor-β (TGF-β) pathway-related protein expression. We also evaluated motor function, sensory function, and the integrity of the blood-spinal cord barrier(BSCB). Results The in situ overexpression of NeuroD1 and Ngn2 in the spinal cord was achieved by specific AAV vectors. This intervention led to a significant increase in cell regeneration and the proportion of cells with neuroblasts and immature neurons cell properties at the injury site(p < 0.0001). Immunofluorescence staining identified astrocytes with neuroblasts and immature neurons cell properties at the site of injury while neuronal marker-specific staining revealed an increased number of mature astrocytes at the injury site. Behavioral assessments showed that the intervention did not improve The BMS (Basso mouse scale) score (p = 0.0726) and gait (p > 0.05), although the treated mice had more sensory sensitivity and greater voluntary motor ability in open field than the non-intervention mice. We observed significant repair of the BSCB at the center of the injury site (p < 0.0001) and a significant improvement in glial scar proliferation. Electrophysiological assessments revealed a significant improvement in spinal nerve conduction (p < 0.0001) while immunostaining revealed that the levels of TGF-β protein at the site of injury in the intervention group were lower than control group (p = 0.0034); in addition, P70 s6 and PP2A related to the TGF-β pathway showed ascending trend (p = 0.0036, p = 0.0152 respectively). Conclusions The in situ overexpression of NeuroD1 and Ngn2 in the spinal cord after spinal cord injury can reprogram astrocytes into neurons and significantly enhance cell regeneration at the injury site. The reprogramming of astrocytes can lead to tissue repair, thus improving the reduced threshold and increasing voluntary movements. This strategy can also improve the integrity of the blood-spinal cord barrier and enhance nerve conduction function. However, the simple reprogramming of astrocytes cannot lead to significant improvements in the striding function of the lower limbs.

Abstract Sex hormones play a pivotal role as endocrine hormones that exert profound effects on the biological characteristics and vascular function of vascular smooth muscle cells (VSMCs). By modulating intracellular signaling pathways, activating nuclear receptors, and regulating gene expression, sex hormones intricately influence the morphology, function, and physiological state of VSMCs, thereby impacting the biological properties of vascular contraction, relaxation, and growth. Increasing evidence suggests that abnormal phenotypic changes in VSMCs contribute to the initiation of vascular diseases, including atherosclerosis. Therefore, understanding the factors governing phenotypic alterations in VSMCs and elucidating the underlying mechanisms can provide crucial insights for refining interventions targeted at vascular diseases. Additionally, the varying levels of different types of sex hormones in the human body, influenced by sex and age, may also affect the phenotypic conversion of VSMCs. This review aims to explore the influence of sex hormones on the phenotypic switching of VSMCs and the development of associated vascular diseases in the human body.

ABSTRACT Diabetic retinopathy (DR) is a complication of diabetes with a complex pathophysiology and multiple factors involved. Recently, it has been found that the upregulation of the renin-angiotensin-aldosterone system (RAAS) leads to overexpression of angiotensin II (Ang II), which induces oxidative stress, inflammation, and angiogenesis in the retina. Therefore, RAAS may be a promising therapeutic target in DR. Notably, RAAS inhibitors are often used in the treatment of hypertension. Still, the potential role and mechanism of DR must be further studied. In this review, we discuss and summarize the pathology and potential therapeutic goals of RAAS in DR. (DR involved Recently reninangiotensinaldosterone renin aldosterone (RAAS Ang II, , II) stress inflammation retina Therefore Notably hypertension Still studied review

Abstract Background In contemporary society, with the accelerated pace of work and life, more and more people feel different degrees of stress. Long-term stress may not only lead to insomnia, but also to mental health problems (e.g., anxiety and depression), which has a significant impact on people's quality of life and mental health. Objective This study primarily investigates the mechanism through which stress affects sleep quality among college students. Methods We conducted research on 1653 Chinese college students using four scales with high reliability and validity: stress, the Pittsburgh Sleep Quality Index, social anxiety, and rumination. Results The study found: (1) Stress can significantly and positively predict sleep quality and rumination; (2) Rumination can positively predict social anxiety; (3) Social anxiety can positively predict sleep quality; (4) Stress can affect sleep quality through social anxiety and rumination separately, and stress can also affect sleep quality through the chained mediation of rumination and social anxiety. Conclusion This study reveals the relationship and mechanisms between stress and sleep quality. It not only deepens the research on the impact of stress on sleep quality but also provides theoretical support and new methods for mental health professionals to help clients improve their sleep quality. In practice, in addition to using some common psychological intervention methods to help individuals reduce stress, we should pay more attention to how to help clients reduce rumination and social anxiety, This is significant in improving the quality of an individual's sleep. society Longterm Long term insomnia e.g., eg e g (e.g. depression, depression , depression) peoples s 165 validity Index found 1 (1 2 (2 3 (3 4 (4 separately practice individual e.g. (e.g 16 ( e.g

Abstract Objective: To explore the relationship between Anion Gap (AG), Albumin Corrected AG (ACAG), and in-hospital mortality of Acute Myocardial Infarction (AMI) patients and develop a prediction model for predicting the mortality in AMI patients. Methods: This was a retrospective cohort study based on the Medical Information Mart for Intensive Care (MIMIC)-III, MIMIC-IV, and eICU Collaborative Study Database (eICU). A total of 9767 AMI patients who were admitted to the intensive care unit were included. The authors employed univariate and multivariable cox proportional hazards analyses to investigate the association between AG, ACAG, and in-hospital mortality; p < 0.05 was considered statistically significant. A nomogram incorporating ACAG and clinical indicators was developed and validated for predicting mortality among AMI patients. Results: Both ACAG and AG exhibited a significant association with an elevated risk of in-hospital mortality in AMI patients. The C-index of ACAG (C-index = 0.606) was significantly higher than AG (C-index = 0.589). A nomo-gram (ACAG combined model) was developed to predict the in-hospital mortality for AMI patients. The nomo-gram demonstrated a good predictive performance by Area Under the Curve (AUC) of 0.763 in the training set, 0.744 and 0.681 in the external validation cohort. The C-index of the nomogram was 0.759 in the training set, 0.756 and 0.762 in the validation cohorts. Additionally, the C-index of the nomogram was obviously higher than the ACAG and age shock index in three databases. Conclusion: ACAG was related to in-hospital mortality among AMI patients. The authors developed a nomogram incorporating ACAG and clinical indicators, demonstrating good performance for predicting in-hospital mortality of AMI patients. Objective , (AG) (ACAG) inhospital hospital (AMI Methods MIMICIII, MIMICIII MIMIC III, III (MIMIC)-III MIMICIV, MIMICIV IV, IV MIMIC-IV eICU. . (eICU) 976 included 005 0 05 0.0 Results Cindex C 0.606 0606 606 0.589. 0589 0.589 589 0.589) nomo gram AUC (AUC 0763 763 0.76 set 0744 744 0.74 0681 681 0.68 0759 759 0.75 0756 756 0762 762 cohorts Additionally databases Conclusion (AG (eICU 97 00 0. 0.60 060 60 058 0.58 58 076 76 0.7 074 74 068 68 0.6 075 75 9 06 6 0.5 5 07 7

RESUMO Objetivo: Comparar a eficácia do Dynesys e do sistema híbrido no tratamento de pacientes com doença degenerativa lombar multissegmentar (DLD). Métodos: Os pacientes envolvidos neste estudo retrospectivo foram divididos em grupos Dynesys (n = 22) e Híbrido (n = 13). Os desfechos clínicos foram avaliados por meio do Oswestry Disability Index (ODI) e da Escala Visual Analógica (EVA). As avaliações radiológicas incluíram radiografia, ressonância nuclear magnética (RNM) e tomografia computadorizada. Ademais, diferentes complicações foram analisadas. Resultados: No acompanhamento final, o ODI e a EVA de todos os grupos melhoraram (p < 0,05), e houve diminuição da amplitude de movimento (ADM) dos segmentos operacionais. No entanto, o grupo Dynesys preservou uma maior extensão da ADM no acompanhamento final (p < 0,05). A ADM do segmento superior adjacente foi ampliada em ambos os grupos (p < 0,05), enquanto as alturas dos discos foram reduzidas no acompanhamento final (p < 0,05). No entanto, o grupo Dynesys apresentou uma redução mais evidente na altura do disco dos segmentos dinâmicos (p < 0,05). Não houve diferença significativa nas complicações entre esses dois grupos (p > 0,05). Conclusão: Neste estudo, resultados satisfatórios semelhantes foram obtidos em ambos os grupos. Ambos os procedimentos cirúrgicos podem ser empregados como tratamentos eficazes para pacientes de meia-idade e fisicamente ativos com LDD multissegmentar. Nível de Evidência III; Estudo Retrospectivo Comparativo. Objetivo DLD. DLD . (DLD) Métodos n 22 13. 13 13) (ODI EVA. (EVA) radiografia RNM (RNM computadorizada Ademais analisadas Resultados p 0,05, 005 0,05 , 0 05 0,05) (ADM operacionais entanto 0,05. Conclusão meiaidade meia idade III Comparativo (DLD 2 1 (EVA 00 0,0 0,

ABSTRACT Objective: To compare effectiveness of Dynesys and hybrid system in treating patients with multi-segmental lumbar degenerative disease (LDD). Methods: Patients involved in this retrospective study were divided into Dynesys (n = 22) and Hybrid (n = 13) groups. Clinical outcomes were evaluated using Oswestry Disability Index (ODI), and Visual Analogue Scale (VAS). Radiologic evaluations included X-ray, MRI, and CT. Furthermore, different complications were analyzed. Results: At the last follow-up, ODI and VAS of each group were improved (p < 0.05), and the range of motion (ROM) of operating segments decreased. However, Dynesys group preserved a larger extent of ROM at the final follow-up (p < 0.05). ROM of the upper adjacent segment was increased in both groups (p < 0.05), while the disc heights were decreased at the final follow-up (p < 0.05). Besides, Dynesys group had a more obvious decrease in the disc height of dynamic segments (p < 0.05). No significant difference existed in complications between both groups (p > 0. 05). Conclusion: In our study, similar satisfactory results were obtained in both groups. Both surgical procedures can be employed as effective treatments for middle-aged and physically active patients with multi-segmental LDD. Level of Evidence III; Retrospective Comparative Study. Objective multisegmental multi segmental LDD . (LDD) Methods n 22 13 ODI, , (ODI) VAS. (VAS) Xray, Xray X ray, ray X-ray MRI CT Furthermore analyzed Results followup, followup follow up, up p 0.05, 005 0.05 0 05 0.05) (ROM However 0.05. Besides 05. 05) Conclusion middleaged middle aged III Study (LDD 2 1 (ODI (VAS 00 0.0

Abstract Cigarette smoke (CS) has been generally recognized as a chief carcinogenic factor in renal cell carcinoma (RCC). The stimulative effect of CS on renal cancer stem cells (RCSCs) has been described previously. The Sonic Hedgehog (SHH) pathway plays an essential role in self-renewal, cell growth, drug resistance, metastasis, and recurrence of cancer stem cells (CSCs). Renal cancer-related gene ΔNp63α is highly expressed in renal epithelial tissues and contributes to the RCSCs characteristics of tumors. The aim of this study was to elucidate the role of ΔNp63α and the SHH pathway on the activity of RCSCs induced by CS through a series of in vivo and in vitro studies. It was shown that in renal cancer tissues, ΔNp63α and RCSCs markers in smokers are expressed higher than that in non-smokers. RCSCs were effectively enriched by tumor sphere formation assay. Besides, CS increased the expression of RCSCs markers and the capability of sphere-forming in vitro and in vivo. Moreover, the SHH pathway was activated, and the specialized inhibitor alleviated the promotion of CS on RCSCs. ΔNp63α activated the SHH pathway and promoted CS-induced enhancement of RCSCs activity. These findings indicate that ΔNp63α positively regulates the activity of CS-induced RCSCs via the SHH pathway. (CS RCC. RCC . (RCC) (RCSCs previously (SHH selfrenewal, selfrenewal self renewal, renewal self-renewal growth resistance metastasis CSCs. CSCs (CSCs) cancerrelated related ΔNpα ΔNp α tumors studies nonsmokers. nonsmokers non smokers. non-smokers assay Besides sphereforming forming Moreover CSinduced (RCC (CSCs

Abstract Objectives: This study aims to elucidate the role of circUSP9X (Circular RNA Ubiquitin Specific Peptidase 9 X-Linked) in the development of venous thrombosis in the lower extremities. Methods: An animal model of Deep Vein Thrombosis (DVT) and a hypoxic model of Human Umbilical Vein Endothelial Cells (HUVECs) treated with Cobalt (II) Chloride (CoCl2) were developed. The expression levels of cir-cUSP9X, microRNA-148b-3p (miR-148b-3p), and SRC Kinase Signaling Inhibitor 1 (SRCIN1) were quantified using quantitative reverse transcription Polymerase Chain Reaction and Western blot analysis. Cell cytotoxicity, viability, apoptosis, and inflammation in HUVECs were assessed via Lactate Dehydrogenase (LDH) assay, MTT assay, flow cytometry, Enzyme-Linked Immunosorbent Assay, and Western blot, respectively. Hematoxylin and Eosin staining were employed for histopathological examination of the venous tissues in the animal model. The interaction between circUSP9X, miR-148b-3p, and SRCIN1 was further explored through dual-luciferase reporter assays and RNA Immunoprecipitation experiments. Results: The present findings reveal a significant upregulation of circUSP9X and SRCIN1 and a concurrent downregulation of miR-148b-3p in DVT cases. Knockdown of circUSP9X or overexpression of miR-148b-3p ameliorated CoCl2-induced apoptosis in HUVECs, reduced LDH release, enhanced cellular viability, and mitigated inflammation. Conversely, overexpression of circUSP9X intensified CoCl2’s cytotoxic effects. The effects of manipulating circUSP9X expression were counteracted by the corresponding modulation of miR-148b-3p and SRCIN1 levels. Additionally, circUSP9X knockdown effectively inhibited the formation of DVT in the mouse model. A competitive binding mechanism of circUSP9X for miR-148b-3p, modulating SRCIN1 expression, was identified. Conclusion: circUSP9X promotes the formation of DVT through the regulation of the miR-148b-3p/SRCIN1 axis. Objectives circUSPX circUSP X Circular XLinked Linked X-Linked extremities Methods (DVT (HUVECs II (II CoCl2 CoCl (CoCl2 developed cir cUSP9X, cUSP9X cUSP cir-cUSP9X microRNA148b3p microRNAbp microRNA 148b 3p b p miR148b3p, miR148b3p miRbp miR , (miR-148b-3p) SRCIN (SRCIN1 analysis cytotoxicity viability (LDH assay cytometry EnzymeLinked Enzyme Assay respectively 3p, dualluciferase dual luciferase experiments Results cases CoCl2induced CoClinduced induced release Conversely CoCl2s CoCls s Additionally identified Conclusion miR148b3p/SRCIN1 miR148b3pSRCIN1 miRbpSRCIN 3p/SRCIN1 miR-148b-3p/SRCIN axis (CoCl cUSPX (miR-148b-3p (SRCIN miR148b3p/SRCIN pSRCIN miR148b3pSRCIN 3pSRCIN1 3p/SRCIN 3pSRCIN

Abstract Histone deacetylase 9 (HDAC9) is a histone deacetylase (HDAC) subtype IIa protein that deacetylates histone 3 (H3), histone 4 (H4), and nonhistone proteins in vivo to alter chromosomal shape and regulate gene transcription. There have been few studies on the regulatory influence of the HDAC9 gene on the differentiation of chicken embryonic stem cells (cESCs) into male germ cells, and the significance of HDAC9 is still unknown. Therefore, we explored the specific role of HDAC9 during differentiation of the cESCs of Jilin Luhua chickens through inhibition or overexpression. In medium supplemented with 10-5 mol/L retinoic acid (RA), cESCs were stimulated to develop into germ cells. HDAC9 and germline marker gene mRNA and protein levels were measured using qRT‒PCR and western blotting. During the differentiation of cESCs into male germ cells, overexpression of the HDAC9 gene greatly increased the mRNA and protein expression levels of the germline marker genes Stra8, Dazl, c-kit, and integrin ɑ6. The HDAC9 inhibitor TMP195 significantly decreased the mRNA and protein expression levels of the above markers. In summary, HDAC9 positively regulates the differentiation of cESCs. HDAC (HDAC9 (HDAC H3, H3 H , (H3) H4, H4 (H4) transcription (cESCs unknown Therefore 105 10 5 10- molL mol L RA, RA (RA) qRTPCR qRT PCR blotting Stra8 Stra Dazl ckit, ckit c kit, kit c-kit ɑ6 ɑ TMP TMP19 markers summary (H3 (H4 1 (RA TMP1 (H

RESUMO Objetivo: Avaliar estudos qualitativos sobre experiências de cuidado de pais cujos filhos têm DM1 e desenvolver estratégias de suporte personalizadas com base nos achados. Método: Revisão sistemática com metassíntese realizada nas bases de dados Cochrane Library, Embase, Scopus, CINAHL, PubMed, Web of Science, CNKI, CBM, VIP e Wanfang. A qualidade foi avaliada por meio dos critérios do JBI, e o método meta-agregativo foi aplicado para categorizar os resultados em subtópicos e agregar em três metatópicos inter-relacionados para compreender as experiências de cuidado dos pais. Resultados: No total, foram encontrados 2.100 artigos, dos quais 15 foram selecionados e analisados. Os três metatópicos identificados foram “Pais enfrentando múltiplos desafios físicos, mentais e de vida”, “Falta de uma gama completa de suporte externo pelos pais” e “A competência do papel de cuidador dos pais aprimorada para se ajustar à nova vida”. Conclusão: É fundamental que os profissionais de saúde reconheçam essas experiências parentais e ofereçam conhecimento específico, treinamento de habilidades e apoio psicológico adaptado às suas necessidades, incluindo treinamento em grupo, intervenções de atenção plena online e maior empatia da equipe médica. Objetivo DM achados Método Library Embase Scopus CINAHL PubMed Science CNKI CBM Wanfang JBI metaagregativo meta agregativo interrelacionados inter relacionados Resultados total 2100 2 100 2.10 artigos 1 analisados Pais físicos vida, vida , vida” Falta vida. . Conclusão específico necessidades grupo médica 210 10 2.1 21 2.

ABSTRACT Objective: To assess qualitative studies on parents’ caregiving experiences whose children have T1DM and develop personalized support strategies based on the findings. Method: A systematic review with meta-synthesis performed in the Cochrane Library, Embase, Scopus, CINAHL, PubMed, Web of Science, CNKI, CBM, VIP, and Wanfang databases. Quality was assessed via the JBI criteria, and meta-aggregative method was applied to categorize the results into subtopics and aggregate into three interrelated meta-topics to understand parents’ caregiving experiences. Results: In total, 2,100 articles were found, out of which 15 were selected and analyzed. The identified three meta-topics were “Parents facing multiple physical, mental and life challenges”, “Parents’ lack of a full range of external support” and “Parents’ caregiving role competency enhanced to adjust to the new life”. Conclusion: it is critical for healthcare professionals to recognize these parental experiences and offer targeted knowledge, skills training, and psychological support tailored to their needs, including group training, online mindfulness interventions, and improved empathy from the medical team. Objective parents TDM T DM findings Method metasynthesis meta synthesis Library Embase Scopus CINAHL PubMed Science CNKI CBM VIP databases criteria metaaggregative aggregative metatopics topics Results total 2100 2 100 2,10 found 1 analyzed Parents physical challenges, challenges , challenges” life. . life” Conclusion knowledge training needs interventions team 210 10 2,1 21 2,

RESUMEN Objetivo: Evaluar estudios cualitativos sobre las experiencias de cuidado de padres cuyos hijos tienen diabetes tipo 1 y desarrollar estrategias de apoyo personalizadas basadas en los hallazgos. Método: Revisión sistemática con metasíntesis realizada en las bases de datos Cochrane Library, Embase, Scopus, CINAHL, PubMed, Web of Science, CNKI, CBM, VIP y Wanfang. La calidad se evaluó utilizando los criterios del JBI y se aplicó el método metaagregativo para categorizar los resultados en subtemas y agregarlos en tres metatemas interrelacionados para comprender las experiencias de cuidado de los padres. Resultados: En total se encontraron 2.100 artículos, de los cuales 15 fueron seleccionados y analizados. Los tres metatemas identificados fueron “Padres que enfrentan múltiples desafíos físicos, mentales y vitales”, “Falta de una gama completa de apoyo externo por parte de los padres” y “Competencia de los padres en la función de cuidado mejorada para adaptarse a la nueva vida”. Conclusión: Es fundamental que los profesionales de la salud reconozcan estas experiencias de los padres y ofrezcan conocimientos específicos, capacitación en habilidades y apoyo psicológico adaptado a sus necesidades, incluida capacitación en grupo, intervenciones de atención plena en línea y una mayor empatía por parte del personal médico. Objetivo hallazgos Método Library Embase Scopus CINAHL PubMed Science CNKI CBM Wanfang Resultados 2100 2 100 2.10 artículos analizados Padres físicos vitales, vitales , vitales” Falta Competencia vida. vida . vida” Conclusión específicos necesidades grupo médico 210 10 2.1 21 2.

Abstract Objective The association between Papillary Thyroid Carcinoma (PTC) and coexistent Hashimoto’s Thyroiditis (HT) was controversial. The purpose of this study was to evaluate the presence of HT exerts any influence on the aggressiveness of PTC, and to establish a nomogram for predicting the possibility of aggressiveness in PTC. Methods 373 consecutive PTC patients with/without coexistent HT from January 2017 to December 2020 were retrospective reviewed. Patients’ clinicopathologic and sonographic characteristics were collected for univariate and multivariate analyses. A nomogram was established based on the risk factors for aggressiveness in PTC. Results Male (p= 0.001), tumor size >1.0 cm (p= 0.046) and lymph node metastasis (p= 0.018) were negatively associated with PTC coexisted with HT, while it was significantly positively associated with the frequence of multifocality (p= 0.010). Univariate and multivariate analyses suggested that age ≥55 years (p= 0.000), male (p= 0.027), HT (p= 0.017), tumor size >1.0 cm (p= 0.015), multifocality (p= 0.041), distance to capsular ≤0 cm (p= 0.050) and blood flow (Grade I: p= 0.044) were independent risk factors for predicting the aggressiveness in PTC. A nomogram according to these predictors was further developed and validated. The receiver operating characteristic curve (AUC = 0.734 and 0.809 for training and validation cohorts, respectively) and decision curve analyses indicated that the nomogram model was clinically useful. The calibration curve revealed that the nomogram exhibited an excellent consistency. Conclusions In this study, the coexistent HT might play a protective role in preventing the proliferation of PTC. Dispensable aggressive treatment may be reduced in PTC by pre-operative identification of sonographic and clinical characteristics and incorporating with the predicted nomogram model. (PTC Hashimotos Hashimoto s (HT controversial 37 withwithout without 201 202 reviewed Patients p (p 0.001, 0001 0.001 , 0 001 0.001) 10 1 >1. 0.046 0046 046 0.018 0018 018 0.010. 0010 0.010 . 010 0.010) 55 ≥5 0.000, 0000 0.000 000 0.000) 0.027, 0027 0.027 027 0.027) 0.017, 0017 0.017 017 0.017) 0.015, 0015 0.015 015 0.015) 0.041, 0041 0.041 041 0.041) ≤ 0.050 0050 050 Grade I 0.044 0044 044 validated AUC 0734 734 0.73 0809 809 0.80 cohorts respectively useful consistency preoperative pre operative 3 20 0.00 00 >1 0.04 004 04 0.01 01 5 ≥ 002 0.02 02 0.05 005 05 073 73 0.7 080 80 0.8 2 0.0 > 07 7 0. 08 8

ABSTRACT Objective: MCM3AP-AS1 has been characterized as an oncogenic long non-coding RNA (lncRNA) in several cancers including papillary thyroid cancer (PTC), but its role in PTC has not been fully elucidated. Considering the critical role of lncRNAs in cancer biology, further functional analysis of MCM3AP-AS1 in PTC may provide novel insights into PTC management. Subjects and methods: Paired tumor and non-tumor tissues were collected from 63 papillary thyroid carcinoma (PTC) patients. Expression levels of MCM3AP-AS1 , miR-218 and GLUT1 in tissue samples were analyzed by qRT-PCR. Cell transfection was performed to explore the interactions among MCM3AP-AS1 , miR-218 and GLUT1 . Cell proliferation assay was performed to evaluate the effects of MCM3AP-AS1 and miR-218 on cell proliferation. Results: MCM3AP-AS1 accumulated to high levels in PTC tissues and was affected by clinical stage. MCM3AP-AS1 showed a positive correlation with GLUT1 across PTC tissues. RNA interaction prediction showed that MCM3AP-AS1 could bind to miR-218 , which can directly target GLUT1 . MCM3AP-AS1 and miR-218 showed no regulatory role regulating the expression of each other, but overexpression of MCM3AP-AS1 upregulated GLUT1 and enhanced cell proliferation. In contrast, overexpression of miR-218 downregulated GLUT1 and attenuated cell proliferation. In addition, miR-218 suppressed the role of MCM3AP-AS1 in regulating the expression of GLUT1 and cell proliferation. Conclusions: MCM3AP-AS1 may serve as a competing endogenous RNA of miR-218 to upregulate GLUT1 in PTC, thereby promoting cell proliferation. The MCM3AP-AS1/miR-218/GLUT1 pathway characterized in the present study might serve as a potential target to treat PTC. Objective MCM3APAS1 MCMAPAS MCM3AP AS1 MCM AP AS MCM3AP-AS noncoding non coding lncRNA (lncRNA elucidated biology management methods nontumor 6 (PTC patients miR218 miR 218 miR-21 GLUT qRTPCR. qRTPCR qRT PCR. PCR qRT-PCR Results stage other contrast addition Conclusions MCM3APAS1/miR218/GLUT1 MCM3APAS1miR218GLUT1 MCMAPASmiRGLUT AS1/miR 218/GLUT1 MCM3AP-AS1/miR-218/GLUT APAS MCM3APAS MCMAP miR21 21 miR-2 MCM3APAS1/miR218/GLUT MCM3APAS1miR218GLUT AS1miR ASmiR 218GLUT1 218/GLUT miR2 2 miR- 218GLUT

Abstract Recurrent miscarriage (RM) seriously affects the physical and mental health of women of childbearing age, and 50% of the causes are unknown. Thus, it is valuable to investigate the causes of unexplained recurrent miscarriage (uRM). Similarities between tumor development and embryo implantation make us realize that tumor studies are informative for uRM. The non-catalytic region of tyrosine kinase adaptor protein 1 (NCK1) is highly expressed in some tumors, and can promote tumor growth, invasion and migration. In this present paper, we firstly explore the role of NCK1 in uRM. We find that the NCK1 and PD-L1 are greatly reduced in peripheral blood mononuclear cells (PBMC) and decidua from patients with uRM. Next, we construct NCK1-knockdown HTR-8/SVneo cells, and find that NCK1-knockdown HTR-8/SVneo cells exhibit reduced proliferation and migration ability. Then we demonstrate that the expression of PD-L1 protein is decreased when the NCK1 is knocked down. In co-culture experiments with THP-1 and differently treated HTR-8/SVneo cells, we observe significantly increased proliferation of THP-1 in NCK1-knockdown group. In conclusion, NCK1 may be involved in RM by regulating trophoblast proliferation, migration, and regulating PD-L1-mediated macrophage proliferation at the maternal-fetal interface. Moreover, NCK1 has the potential to be a new predictor and therapeutic target. (RM age 50 unknown Thus uRM . (uRM) noncatalytic non catalytic NCK (NCK1 tumors growth paper PDL1 PDL PD L1 L PD-L PBMC (PBMC Next NCK1knockdown NCKknockdown knockdown HTR8/SVneo HTR8SVneo HTRSVneo HTR 8/SVneo 8 SVneo ability down coculture co culture THP1 THP THP- group conclusion PDL1mediated PDLmediated mediated maternalfetal maternal fetal interface Moreover target 5 (uRM (NCK HTR8 8SVneo

ABSTRACT The issue of reducing tillage resistance and lowering energy consumption has become increasingly relevant. Strip tillage has a positive impact on soil protection and energy consumption reduction. This study analysed the tillage resistance components of rotary blades and their relationship with operating parameters and developed a mathematical relationship between tillage resistance and energy consumption (power and fuel consumption). We summarised tillage resistance into three parts: air resistance, lateral rotational friction resistance (divided into horizontal and vertical resistance), and shear force. By theoretical analysis and coupled simulation tests 11 (DEM-CFD), we obtained an air resistance, lateral rotational friction, and shear force of 0.0883 N, 97.21 N, and 893.496 N, respectively, accounting for 0.01%, 9.81%, and 90.18% of tillage resistance. Meanwhile, we found that horizontal resistance decreased linearly with the increase of angular speed, which was the opposite of vertical resistance. Horizontal and vertical resistance increased linearly with the increase in contact area. Shear force increased significantly with angular speed. Fuel consumption in the field test and tillage resistance and power consumption in coupled simulation was formed to build a correspondence point, and a mathematical relationship between tillage resistance, torque, and power and fuel consumption was established. relevant reduction consumption. . consumption) parts divided , resistance) 1 DEMCFD, DEMCFD DEM CFD (DEM-CFD) 00883 0 0883 0.088 N 9721 97 21 97.2 893496 893 496 893.49 respectively 001 01 0.01% 981 9 81 9.81% 9018 90 18 90.18 Meanwhile speed area point torque established (DEM-CFD 0088 088 0.08 972 2 97. 89349 89 49 893.4 00 0.01 98 8 9.81 901 90.1 008 08 0.0 8934 4 893. 9.8 90. 0. 9.