Abstract Objective Monitoring the disease status of Epstein-Barr virus (EBV)-related hemophagocytic lymphohistiocytosis (HLH) patients is crucial. This study aimed to investigate the different strategies and outcomes of patients with EBV-HLH and re-elevated EBV-DNA. Method A retrospective analysis was conducted on 20 patients diagnosed with EBV-HLH. Clinical features, laboratory tests, treatments, plasma EBV-DNA levels, and outcomes were assessed. Three cases were highlighted for detailed analysis. Results Nine of the 20 patients had a re-elevation of EBV-DNA during treatment, and 55.5 % (5/9) experienced relapses. Patients with persistently positive plasma EBV-DNA (n = 4) and those with re-elevated EBV-DNA after conversion (n = 9) showed a significantly higher relapse rate compared to those with persistently negative EBV-HLH (n = 7) (p < 0.05). Among the highlighted cases, Case 1 exhibited plasma EBV-DNA re-elevation after four weeks of treatment without relapse, maintaining stability with the original treatment regimen, and eventually, his plasma EBV-DNA turned negative. In Case 2, plasma EBV-DNA was elevated again with a recurrence of HLH after L-DEP. Consequently, she underwent allogeneic hematopoietic stem cell transplantation and eventually achieved complete remission (CR) with negative plasma EBV-DNA. Case 3 experienced plasma EBV-DNA re-elevation after L-DEP but remained in CR, discontinuing chemotherapy without relapse. Conclusion The re-elevation of plasma EBV-DNA during EBV-HLH treatment poses challenges in determining disease status and treatment strategies. Optimal management decisions require a combination of the level of elevated EBV-DNA, the intensity of hyperinflammation, and the patient's immune function. EpsteinBarr Epstein Barr EBVrelated EBV related (HLH crucial EBVHLH reelevated re EBVDNA. EBVDNA DNA. DNA 2 EBVHLH. HLH. features tests treatments levels assessed reelevation elevation 555 55 5 55. 5/9 59 9 (5/9 relapses n 4 7 p 0.05. 005 0.05 . 0 05 0.05) regimen LDEP. LDEP L DEP. DEP Consequently CR (CR EBVDNA, DNA, hyperinflammation patient s function 5/ (5/ 00 0.0 (5 0. (

Abstract Background Cutaneous squamous cell carcinoma (CSCC) is one of the most common types of skin cancer worldwide. Therefore, the identification of biomarkers associated with CSCC progression could aid in the early detection of high-risk squamous cell carcinoma and the development of novel therapeutic strategies. Objective This study aimed to investigate the expression patterns of silent mating type Information Regulation 2 homolog 6 (SIRT6) in CSCC and its clinical significance. Methods The protein expression level of SIRT6 in tissues was detected by immunohistochemistry, and the correlation between SIRT6 expression and clinicopathological parameters in CSCC patients was analyzed. The relative expression of SIRT6 in CSCC cell lineage and tissue specimens was determined by western blotting and PCR. The effect of SIRT6 silencing on cell proliferation was evaluated using cell counting kit 8. Wound healing, transwell method, and flow cytometry were used to investigate the migration, invasion, and cell cycle distribution/apoptosis of CSCC cells after SIRT6 silencing, respectively. Western blot was used to detect the expression of EMT (Epithelial-Mesenchymal Transition), cycle, apoptosis, and other related proteins. Results The high expression of SIRT6 was correlated with the location of cancer tissue and Broder staging in CSCC patients. Knockdown of SIRT6 inhibited the proliferation, migration, invasion and EMT of CSCC cells, and promoted their apoptosis, with cells blocked in G1 phase. Study limitations No animal experiments were conducted to further verify the results. Conclusion Decreased expression of SIRT6 can inhibit the occurrence and development of CSCC. (CSCC worldwide Therefore highrisk risk strategies SIRT (SIRT6 significance immunohistochemistry analyzed PCR 8 healing method migration distributionapoptosis distribution apoptosis respectively EpithelialMesenchymal Epithelial Mesenchymal Transition, Transition , Transition) proteins G phase results (SIRT

Abstract The cure rates for osteosarcoma have remained unchanged in the past three decades, especially for patients with pulmonary metastasis. Thus, a new and effective treatment for metastatic osteosarcoma is urgently needed. Anlotinib has been reported to have antitumor effects on advanced osteosarcoma. However, both the effect of anlotinib on autophagy in osteosarcoma and the mechanism of anlotinib-mediated autophagy in pulmonary metastasis are unclear. The effect of anlotinib treatment on the metastasis of osteosarcoma was investigated by transwell assays, wound healing assays, and animal experiments. Related proteins were detected by western blotting after anlotinib treatment, ATG5 silencing, or ATG5 overexpression. Immunofluorescence staining and transmission electron microscopy were used to detect alterations in autophagy and the cytoskeleton. Anlotinib inhibited the migration and invasion of osteosarcoma cells but promoted autophagy and increased ATG5 expression. Furthermore, the decreases in invasion and migration induced by anlotinib treatment were enhanced by ATG5 silencing. In addition, Y-27632 inhibited cytoskeletal rearrangement, which was rescued by ATG5 overexpression. ATG5 overexpression enhanced epithelial-mesenchymal transition (EMT). Mechanistically, anlotinib-induced autophagy promoted migration and invasion by activating EMT and cytoskeletal rearrangement through ATG5 both in vitro and in vivo. Our results demonstrated that anlotinib can induce protective autophagy in osteosarcoma cells and that inhibition of anlotinib-induced autophagy enhanced the inhibitory effects of anlotinib on osteosarcoma metastasis. Thus, the therapeutic effect of anlotinib treatment can be improved by combination treatment with autophagy inhibitors, which provides a new direction for the treatment of metastatic osteosarcoma. decades Thus needed However anlotinibmediated mediated unclear assays experiments ATG silencing cytoskeleton expression Furthermore addition Y27632 Y 27632 Y-2763 epithelialmesenchymal epithelial mesenchymal EMT. . (EMT) Mechanistically anlotinibinduced vivo inhibitors Y2763 2763 Y-276 (EMT Y276 276 Y-27 Y27 27 Y-2 Y2 2 Y-

Abstract NCED1 (9-cis-epoxy carotenoid dioxygenase) plays important roles in controlling ABA levels and drought stress tolerance. The relationships between sequence polymorphisms of the TaNCED1 gene and drought resistance in wheat were analysed. Four allelic variations of the TaNCED1-5B generated to drought resistance were identified. Four groups of KASP (kompetitive allele-specific PCR) markers were developed based on the four alleles and were verified in a natural population of 311 wheat cultivars. The distribution of the four alleles of TaNCED1-5B in the natural population was clarified. Among the allelic variations, 84.62% of the wheat varieties with TT/-/C/-,TT/C/C/-,TT/-/C/G or TT/C/C/G haplotypes were dryland cultivars. The common feature of the above four haplotypes is that they all contain the TT (98) and C (343) alleles at the TaNCED1-5B-TT/CG (98) and TaNCED1-5B-C/T (343) loci, which indicated that these two locis had highly positive correlation with drought resistance in wheat. NCED 9cisepoxy cisepoxy 9 cis epoxy dioxygenase tolerance TaNCED analysed TaNCED15B TaNCEDB 5B B identified kompetitive allelespecific allele specific PCR 31 cultivars clarified 8462 84 62 84.62 TT//C/,TT/C/C/,TT//C/G TTCTTCCTTCG TT/ /C/ ,TT/C/C/ ,TT/ /C/G G TTCCG 98 (98 343 (343 TaNCED15BTT/CG TaNCED15BTTCG TaNCEDBTTCG TT/CG CG TaNCED15BC/T TaNCED15BCT TaNCEDBCT C/T T loci 3 846 8 6 84.6 /C TTCC ,TT/C/C ,TT (9 34 (34 TaNCED15BTT BTT BTTCG TTCG TaNCED15BC BC BCT CT 84. ( (3 TaNCEDBTT TaNCEDBC

Abstract Hanyou 3015 is a new Indica hybrid of water-saving and drought-resistance rice (WDR), combining rapid maturation and high yield potential. Whole genome sequencing results show that Hanyou 3015 carries many superior alleles that could explain its drought tolerance, high water use efficiency, rapid maturation, and wide adaptability. 301 watersaving saving droughtresistance resistance WDR, WDR , (WDR) potential tolerance efficiency adaptability 30 (WDR 3

ABSTRACT Despite good hepatitis B virus (HBV) inhibition by nucleoside analogs (NAs), cases of hepatocellular carcinoma (HCC) still occur. This study proposed a non-invasive predictive model to assess HCC risk in patients with chronic hepatitis B (CHB) receiving NAs treatment. Data were obtained from a hospital-based retrospective cohort registered on the Platform of Medical Data Science Academy of Chongqing Medical University, from 2013 to 2019. A total of 501 patients under NAs treatment had their FIB-4 index updated semiannually by recalculation based on laboratory values. Patients were divided into three groups based on FIB-4 index values: < 1.45, 1.45–3.25, and ≥ 3.25. Subsequently, HCC incidence was reassessed every six months using Kaplan-Meier curves based on the updated FIB-4 index. The median follow-up time of CHB patients after receiving NAs treatment was 2.5 years. HCC incidences with FIB-4 index < 1.45, 1.45–3.25, and ≥ 3.25 were 1.18%, 1.32%, and 9.09%, respectively. Dynamic assessment showed that the percentage of patients with FIB-4 index < 1.45 significantly increased semiannually (P < 0.001), and of patients with FIB-4 index ≥ 3.25 significantly decreased (P < 0.001). HCC incidence was the highest among patients with FIB-4 index ≥ 3.25. The FIB-4 index effectively predicted HCC incidence, and its dynamic assessment could be used for regular surveillance to implement early intervention and reduce HCC risk. HBV (HBV NAs, , (NAs) (HCC occur noninvasive non invasive (CHB hospitalbased hospital University 201 2019 50 FIB4 FIB 4 FIB- values 145 1 45 145325 3 25 1.45–3.25 325 Subsequently KaplanMeier Kaplan Meier followup follow up 2 5 2. years 3.2 118 18 1.18% 132 32 1.32% 909 9 09 9.09% respectively 1.4 P 0.001, 0001 0.001 0 001 0.001) 0.001. . (NAs 20 14 14532 1.45–3.2 3. 11 1.18 13 1.32 90 9.09 1. 000 0.00 00 1453 1.45–3. 1.1 1.3 9.0 0.0 1.45–3 9. 0. 1.45–

Abstract Histone deacetylase 9 (HDAC9) is a histone deacetylase (HDAC) subtype IIa protein that deacetylates histone 3 (H3), histone 4 (H4), and nonhistone proteins in vivo to alter chromosomal shape and regulate gene transcription. There have been few studies on the regulatory influence of the HDAC9 gene on the differentiation of chicken embryonic stem cells (cESCs) into male germ cells, and the significance of HDAC9 is still unknown. Therefore, we explored the specific role of HDAC9 during differentiation of the cESCs of Jilin Luhua chickens through inhibition or overexpression. In medium supplemented with 10-5 mol/L retinoic acid (RA), cESCs were stimulated to develop into germ cells. HDAC9 and germline marker gene mRNA and protein levels were measured using qRT‒PCR and western blotting. During the differentiation of cESCs into male germ cells, overexpression of the HDAC9 gene greatly increased the mRNA and protein expression levels of the germline marker genes Stra8, Dazl, c-kit, and integrin ɑ6. The HDAC9 inhibitor TMP195 significantly decreased the mRNA and protein expression levels of the above markers. In summary, HDAC9 positively regulates the differentiation of cESCs. HDAC (HDAC9 (HDAC H3, H3 H , (H3) H4, H4 (H4) transcription (cESCs unknown Therefore 105 10 5 10- molL mol L RA, RA (RA) qRTPCR qRT PCR blotting Stra8 Stra Dazl ckit, ckit c kit, kit c-kit ɑ6 ɑ TMP TMP19 markers summary (H3 (H4 1 (RA TMP1 (H

ABSTRACT CONTEXT: Scrub typhus, caused by Orientia tsutsugamushi, has a wide range of clinical manifestations, including meningoencephalitis, acute renal failure, pneumonitis, myocarditis, and septic shock. However, there are no documented cases of scrub typhus with hypokalemia. In this report, we present a case of scrub typhus with hypokalemia and multiple organ failure syndrome, highlighting the importance of electrolyte imbalance in patients with scrub typhus. CASE REPORT: A 59-year-old woman presented to the emergency department with abdominal pain that had been present for 1 day. On admission, the physical examination and laboratory test results indicated that the patient had renal, liver, and circulatory failure, and hypokalemia. She developed meningitis and disseminated intravascular coagulation during hospitalization. She recovered with appropriate management, and was discharged on day 17. CONCLUSION: This report highlights the potential for atypical presentations of scrub typhus, including a previously undocumented association with hypokalemia. Although the contribution of hypokalemia to the patient’s clinical course remains uncertain, this case underscores the importance of considering electrolyte imbalance in the management of patients with scrub typhus. Further research is warranted to better understand the relationship between scrub typhus and electrolyte imbalance. CONTEXT tsutsugamushi manifestations meningoencephalitis pneumonitis myocarditis shock However syndrome REPORT 59yearold yearold 59 year old admission liver hospitalization 17 CONCLUSION s uncertain 5

ABSTRACT: Bovine viral diarrhea virus (BVDV) can cause acute and persistent infections in cattle, resulting in significant economic losses to the livestock industry each year. Targeted antiviral therapy is an effective strategy. This study was based on network pharmacology, molecular docking techniques and in vitro studies to investigate quercetin’s mechanism in treating bovine viral diarrhea/mucosal disease (BVD-MD). The network topology analysis was carried out using Cytoscape 3.9.0 software to construct the network of “Chinese medicine ingredients-target-diseases”. Protein interactions were explored and analyzed using the String system (PPI). GO and KEGG pathway analysis of the intersected targets was performed using Bioconductor software. The molecular docking and molecular dynamics simulation methods were used to reveal the degree of binding of quercetin to key target genes. Western blot and indirect immunofluorescence were used to characterize the antiviral effects of quercetin. This study utilized network pharmacological analysis, identifying 22 targets associated with BVD-MD. The results of the KEGG pathway showed that quercetin was closely related to Ras and MAPK signaling pathways of BVD-MD. Molecular docking results showed that SRC, NS5B, NOX4 and XDH were the key targets of quercetin in the treatment of BVD-MD. Through network pharmacology, molecular docking and in vitro experiments, quercetin was demonstrated to combat bovine viral diarrhea mucosal disease through key targets of SRC, MAPK1, GSK3B, NS5B and E2. Molecular dynamics analysis showed that quercetin exhibited complex stability with NS5B. This study provides a theoretical and experimental basis for quercetin treatment of BVD-MD and later drug development. ABSTRACT BVDV (BVDV cattle year strategy pharmacology quercetins s diarrheamucosal BVDMD. BVDMD BVD MD . (BVD-MD) 390 3 9 0 3.9. Chinese ingredientstargetdiseases. ingredientstargetdiseases ingredients diseases ingredients-target-diseases” PPI. PPI (PPI) genes 2 MD. SRC NSB NS B NOX experiments MAPK1 GSK3B GSKB GSK E2 E development (BVD-MD 39 3.9 ingredients-target-diseases (PPI 3.

ABSTRACT To study the damage evolution behavior and damage constitutive model of selective-laser-melted (SLMed) Ti6Al4V alloy under different heat treatment temperatures, tensile tests were conducted. The effect of heat treatment temperature on microstructure and mechanical properties of SLMed Ti6Al4V alloy is discussed. Elastic modulus was selected to characterize damage, and the damage evolution law of SLMed Ti6Al4V alloy was quantitatively analyzed, establishing the damage evolution equation. Based on the theory of continuous damage mechanics, the Ramberg-Osgood model was introduced to establish the damage constitutive model of SLMed Ti6Al4V alloy under different heat treatment temperatures. The results show that with increasing heat treatment temperature, the acicular α′ phase in the microstructure of SLMed Ti6Al4V alloy is gradually transformed into α + β phase, the α phase is gradually coarsened, the volume fraction of β phase gradually increased. The microhardness and tensile strength of the SLMed Ti6Al4V alloy decreased from 376.44 HV and 1171.5 MPa to 317.31 HV and 790.9 MPa, while elongation increased from 6.6% to 13.6% and then decreased to 11.1%. The critical strain in the as-deposited sample is 0.009, entering the rapid damage accumulation stage first, whereas heat-treated samples lag significantly. The error between model and experimental results is within 7%. selectivelasermelted selective laser melted (SLMed TiAlV Ti Al V temperatures conducted discussed analyzed equation mechanics RambergOsgood Ramberg Osgood coarsened 37644 376 44 376.4 11715 1171 5 1171. 31731 317 31 317.3 7909 790 9 790. 66 6 6.6 136 13 13.6 111 11 1 11.1% asdeposited as deposited 0009 0 009 0.009 first heattreated treated significantly 7 7% 3764 37 4 376. 117 3173 3 317. 79 6. 13. 11.1 000 00 0.00 11. 0.0 0.

ABSTRACT Pure magnesium (Mg) is a promising biodegradable biomaterial due to its excellent biocompatibility and appropriate elastic modulus. However, its high corrosion rate and insufficient mechanical properties limit its broader application. In this study, pure Mg is processed using high-pressure torsion (HPT) for 1, 2, and 5 turns. The evolution of the microstructure during the HPT process, as well as the microhardness distribution and corrosion resistance of the material after HPT, are investigated. The results reveal that HPT significantly refines the grain size of pure Mg, reducing it from about 5 mm to about 1 μm. This grain refinement increased the microhardness of pure Mg by 35%, and the microhardness values were uniformly distributed after 5 turns. The corrosion resistance of pure Mg improves with increasing number of HPT turns. This is indicated by a positive shift in the corrosion potential and a reduction in the rate of hydrogen evolution. The refined grain contributes to the formation of a denser and more uniform corrosion product layer, which enhances the protection of pure Mg substrates. (Mg modulus However application study highpressure pressure (HPT 2 turns process investigated μm 35 35% layer substrates 3

Abstract Objective: To investigate the efficacy of cluster nursing intervention based on Enhanced Recovery After Surgery (ERAS) for xerostomia in chronic rhinosinusitis after nasal endoscopic surgery. Methods: A total of 80 patients with chronic rhinosinusitis who underwent functional nasal endoscopic surgery between January 2020 and December 2021 were selected and randomly divided into a control group (n = 40) and an experimental group (n = 40). Patients in the control group were treated with general nursing, while ERAS-based cluster nursing intervention was adopted for the experimental group, in addition to general nursing. Xerostomia stage and comfort level were observed at 2h, 6h, 24h and 48h after surgery; negative emotions before and after nursing were also observed. Results: After the intervention, the xerostomia stage and comfort level at 6, 24 and 48 after surgery were higher in the experimental group (p < 0.05). Negative emotions in the experimental group were lower after nursing (p < 0.001). The self-rating depression scale and self-rating anxiety scale scores increased after nursing in both two groups (p < 0.05). Conclusion: Enhanced recovery after surgery-based cluster nursing intervention can alleviate xerostomia, improve patients’ comfort levels, reduce their negative emotions and accelerate postoperative recovery. Level of evidence: 2. Objective ERAS (ERAS Methods 8 202 n 40 40. . ERASbased 2h h 6h Results 6 2 4 p 0.05. 005 0.05 0 05 0.05) 0.001. 0001 0.001 001 0.001) selfrating self rating Conclusion surgerybased levels evidence 20 00 0.0 000 0.00 0.

In this work, we adopt the three-order perturbation formulae for g-factors (g‖, g⏊) of d1 ions in the octahedral environment to calculate the g-factors of W5+ ions in tungsten phosphate glasses containing lithium (P2O5-Li2W4O-Li2O). In the light of the high valence state of the studied W5+ centers and hence the strong covalency of the studied octahedral [WO6]7- cluster, we consider the contributions to g-factors from the ligand orbital and spin-orbit (SO) coupling interactions based on the cluster approach. The required tetragonal crystal-field parameters are calculated from the local structure of W5+ ions based on the superposition model. According to the theoretical calculations, we find that the octahedral [WO6]7- clusters possess the tetragonally compressed distortion with a shorter W-O bond length (≈ 1.54 Å) and a longer one (≈ 2.26 Å) along C4 axis and four normal W-O bond length (≈ 1.94 Å) in the perpendicular plane, which infers that the W5+ ions are in the form of tungstyl ions (i.e., WO3+). Based on the local structural data, the theoretical values of g‖ and g⏊ agree well with the experimental values.

Abstract Background stewart-treves syndrome (STS) is an angiosarcoma associated with chronic lymphedema. Objectives This article analyses the characteristics of twenty-two patients and proposes active intervention in lymphedema and the early diagnosis of STS. Methods Twenty-two patients with STS were diagnosed at the centre over an 11-year period. Clinical manifestations, a series of conventional analyses, and histopathology were used to study these cases retrospectively. Results The age range of 22 patients with STS was 15 to 78 years. The main clinical manifestations included multiple skin and subcutaneous nodules and scattered red or purplish-red rashes in the lymphoedematous limbs. These patients often showed clinical symptoms such as lymphedema, weakness, emaciation, pain, mass, lymphadenopathy and so on. The positive rates of ultrasonography, MRI and radionuclide imaging were 66.7% (6/9), 92.3% (12/13) and 18.2% (2/11), respectively. The main points regarding active intervention in lymphedema and early diagnosis of STS were summarized. Study limitations Since this was a retrospective study, the main points summarized by the author need to be further quantified in clinical work to guide the diagnosis of this kind of disease more conveniently. In addition, further clinical trials are needed to evaluate the role of lymphedema in the occurrence and development of malignant tumors. Conclusions STS can appear in lymphoedematous tissue many years after lymphedema onset. To avoid delays in the diagnosis and therapy of STS, physicians should actively look for signs or symptoms of malignant lymphedema during the follow-up period and promptly manage patients developing problems. stewarttreves stewart treves (STS twentytwo twenty two Twentytwo Twenty 11year year 11 retrospectively 2 1 7 purplishred purplish limbs weakness emaciation pain mass on ultrasonography 667 66 66.7 6/9, 69 6/9 , 6 9 (6/9) 923 92 3 92.3 12/13 1213 12 13 (12/13 182 18 18.2 2/11, 211 2/11 (2/11) respectively conveniently addition tumors onset followup follow up problems 66. 6/ (6/9 92. 12/1 121 (12/1 18. 21 2/1 (2/11 (6/ 12/ (12/ 2/ (2/1 (6 (12 (2/ ( (1 (2

SUMMARY: The objective of this study was to evaluate the changes of head and cervical spine posture of skeletal class malocclusion in adolescent with maxillary protraction. Thirty cases of skeletal class malocclusion were randomly selected from the Stomatological Hospital of Shanxi Medical University. High-quality lateral cephalograms were collected including pre- and posttreatment to compare the changes of head and cervical spine posture. Data were processed using SPSS 26.0 statistical software. The paired-t test was used to compare pre- and posttreatment mean angular measurements.A significant difference in the SNA(p<0.001), SNB(p<0.01), and ANB(p<0.001) between T1 and T2 showed an improvement in the sagittal relationships. A significant change was observed in middle cervical spine posture, while upper cervical spine posture variables showed no significant difference after treatment. Skeletal class with maxillary protraction appliance not only led to the improvement of sagittal relationship, but also changed the middle cervical spine posture.

RESUMEN: El objetivo de este estudio fue evaluar los cambios en la postura de la cabeza y la columna cervical debido a la maloclusión clase esquelética en adolescentes con protracción maxilar. Treinta casos de maloclusión de clase esquelética fueron seleccionados al azar del Hospital Estomatológico de la Universidad Médica de Shanxi. Se recogieron cefalogramas laterales de alta calidad, incluidos el tratamiento previo y posterior, para comparar los cambios en la postura de la cabeza y la columna cervical. Los datos se procesaron con el software estadístico SPSS 26.0. Se utilizó la prueba t pareada para comparar las medidas angulares medias antes y después del tratamiento. Una diferencia significativa en SNA (p <0,001), SNB (p <0,01) y ANB (p <0,001) entre T1 y T2 mostró una mejora en las relaciones sagitales. Se observó un cambio significativo en la postura de la columna cervical media, mientras que las variables de postura de la columna cervical superior no mostraron diferencias significativas después del tratamiento. La clase esquelética con aparato de protracción maxilar no solo condujo a la mejora de la relación sagital, sino que también cambió la postura de la columna cervical media.