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Proposal of a screening instrument for autism spectrum disorder in children (Mini-TEA Scale) MiniTEA Mini TEA Scale
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Forcelini, Cassiano Mateus
; Ampese, Regina
; Melo, Helena Younes de
; Pasin, Camila Pereira Neubauer
; Pádua, José Renato Donadussi
; Spanholo, Camila Boschetti
; Hoffmann, Francine Ehrhardt
; Diniz, Júlia Breitenbach
; Capponi, Laís Cristine Zanella
; Souza, Luiza
; Zortea, Maxciel
.
Abstract Background Autism spectrum disorder (ASD) requires trained professionals for its adequate diagnosis. There is a shortage of such professionals in Brazil. Screening tools could identify priority cases. The only instrument for that in Brazilian Portuguese is employed for toddlers up to 2.5 years old. Objective The Mini-TEA scale was conceived and tested as a screening for children from 2.5 to 12 years old. Methods After local ethics committee's approval, this study was conducted from December 2022 to April 2023 in the Associação de Pais e Amigos dos Excepcionais, Passo Fundo/RS, of invitations to children's parents/relatives who were under evaluation for ASD and by local advertisement. Inclusion criteria were age from 2.5 to 12 years old; consent from the child's legal guardians. 75 children's parents/relatives were interviewed using the 15-item Mini-TEA scale. After that, children were evaluated for the diagnosis of ASD by a pediatric neurologist. Sensibility and specificity for ASD diagnosis along the Mini-TEA scores were measured. Experts and target population evaluated the validity/reliability of the Mini-TEA scale. The reproducibility of the scores was assessed about 40 days later. Results From the 75 participants, 28 received a diagnosis of ASD. Scores ≥ 10 on the Mini-TEA scale require further evaluation of the children (sensitivity 100%; specificity 68%). Content validity coefficient (CVC) rendered values > 0.80 (acceptable). Test-retest analyzes with the intraclass correlation coefficient (ICC) indicated excellent reliability (> 0.90). The time spent for applying the screening was about 10 minutes. Conclusion The Mini-TEA scale presents as an easy tool for screening ASD among children. (ASD Brazil cases 25 2 5 2. old MiniTEA Mini TEA 1 committees committee s approval 202 Excepcionais FundoRS Fundo RS Fundo/RS childrens parentsrelatives parents relatives advertisement childs child guardians 7 15item item 15 neurologist measured validityreliability 4 later participants sensitivity 100% 100 68%. 68 68% . 68%) CVC (CVC 080 0 80 0.8 acceptable. acceptable (acceptable) Testretest Test retest ICC (ICC ( 0.90. 090 0.90 90 0.90) minutes 20 6 08 8 0. (acceptable 09 0.9 9
Resumo Antecedentes O transtorno do espectro autista (TEA) requer profissionais treinados para o diagnóstico, escassos no Brasil. Instrumentos de triagem poderiam identificar casos prioritários para avaliação. O único em português brasileiro é empregado para crianças até 30 meses de idade. Objetivo A escala Mini-TEA foi concebida e testada como triagem para crianças entre 2,5 e 12 anos. Métodos Estudo foi conduzido de dezembro de 2022 a abril de 2023 na Associação de Pais e Amigos dos Excepcionais (APAE) de Passo Fundo/RS, após a aprovação bioética local. O recrutamento consistiu em convite aos familiares de crianças que estavam sendo avaliadas para TEA e por divulgação local. Os critérios de inclusão foram idade entre 2,5 e 12 anos e consentimento do guardião legal. Familiares de 75 crianças foram entrevistados com a escala Mini-TEA (15 itens). Depois, as crianças foram avaliadas para o diagnóstico de TEA por neuropediatra. A sensibilidade e a especificidade do diagnóstico de TEA com os escores da Mini-TEA foram mensuradas. A validade e a confiabilidade da escala Mini-TEA foram avaliadas por experts e pela população alvo. A reprodutibilidade dos escores foi medida após ± 40 dias. Resultados Dos 75 participantes, 28 receberam diagnóstico de TEA. Escores ≥ 10 na escala Mini-TEA requerem avaliação das crianças (sensibilidade 100%; especificidade 68%). O coeficiente de validação de conteúdo (CVC) rendeu valores > 0,80 (aceitável). Análises de teste-reteste com coeficiente de correlação intraclasse (ICC) indicou excelente confiabilidade (> 0,90). O tempo gasto para a triagem foi cerca de 10 minutos. Conclusão A escala Mini-TEA constitui ferramenta breve e fácil para triagem de TEA em crianças. (TEA Brasil 3 MiniTEA Mini 25 2 5 2, 1 202 APAE (APAE FundoRS Fundo RS Fundo/RS local legal 7 15 (1 itens. itens . itens) Depois neuropediatra mensuradas alvo 4 dias participantes 100% 100 68%. 68 68% 68%) CVC (CVC 080 0 80 0,8 aceitável. aceitável (aceitável) testereteste teste reteste ICC (ICC ( 0,90. 090 0,90 90 0,90) minutos 20 6 08 8 0, (aceitável 09 0,9 9
2.
Persistence of S-metolachlor in the soil as affected by moisture content Smetolachlor S metolachlor
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Avila, Luis A.
; Zemolin, Carla R.
; Fipke, Marcus V.
; Cassol, Guilherme V.
; Cassol, Luciano L.
; Cassol, Ana P. V.
; Zanella, Renato
; Camargo, Edinalvo R.
.
Abstract Background: Several factors may affect herbicide fate in the soil, including soil moisture which can affect herbicide availability and degradation and mixture with other degradable herbicides. Objective: The objectives of this research were to evaluate the effects of soil moisture content and association with glyphosate on S-metolachlor persistence in lowland soil. Methods: Greenhouse experiments were conducted in 2011 and repeated in 2012 using a randomized complete block design in a factorial arrangement (3×3×5) with four replications. Factor A included three soil moisture contents (air-dried, water holding capacity, and saturated), and factor B included two herbicide combinations (S-metolachlor; S-metolachlor + glyphosate) plus an untreated check. Factor C included five intervals between application and sowing of the bioindicator species (rice): 150, 120, 90, 60, and 30 days before sowing. Herbicide injury, height in rice plants, and herbicide concentration in soil was evaluated. Results: S-metolachlor concentration and injury to rice were higher under dry soil conditions regardless of application timing. In contrast, rice injury was significantly lower in the soil saturated condition. The association with glyphosate did not affect S-metolachlor persistence in lowland soil. Conclusion: These results indicate that S-metolachlor exhibits shorter persistence under saturated soil conditions; and indicated that drought periods following S-metolachlor applications may increase carryover to sensitive crops planted in rotation. Background herbicides Objective Smetolachlor S metolachlor Methods 201 3×3×5 335 3 5 (3×3×5 replications airdried, airdried air dried, dried (air-dried capacity saturated, , saturated) (S-metolachlor check (rice) 150 120 90 60 plants evaluated Results timing contrast condition Conclusion rotation 20 3×3× 33 (3×3× (rice 15 12 9 6 2 3×3 (3×3 1 3× (3× (3 (
3.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
; Flechtmann, Carlos H.W.
; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
; Oliveira, Ivo de S.
; Delabie, Jacques H.C.
; Oliveira, Jader de
; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
; Morselli, João P.
; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
; Araújo, Marcel S. de
; Monné, Marcela L.
; Labruna, Marcelo B.
; Santis, Marcelo D. de
; Duarte, Marcelo
; Knoff, Marcelo
; Nogueira, Marcelo
; Britto, Marcelo R. de
; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
; Justo, Marcia C.N.
; Botelho, Marcia J.C.
; Couri, Márcia S.
; Borges-Martins, Márcio
; Felix, Márcio
; Oliveira, Marcio L. de
; Bologna, Marco A.
; Gottschalk, Marco S.
; Tavares, Marcos D.S.
; Lhano, Marcos G.
; Bevilaqua, Marcus
; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
; Santos, Maria E.A. dos
; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
; Terossi, Mariana
; Bartz, Marie L.C.
; Barbosa, Marina F. de C.
; Loeb, Marina V.
; Cohn-Haft, Mario
; Cupello, Mario
; Martins, Marlúcia B.
; Christofersen, Martin L.
; Bento, Matheus
; Rocha, Matheus dos S.
; Martins, Maurício L.
; Segura, Melissa O.
; Cardenas, Melissa Q.
; Duarte, Mércia E.
; Ivie, Michael A.
; Mincarone, Michael M.
; Borges, Michela
; Monné, Miguel A.
; Casagrande, Mirna M.
; Fernandez, Monica A.
; Piovesan, Mônica
; Menezes, Naércio A.
; Benaim, Natalia P.
; Reategui, Natália S.
; Pedro, Natan C.
; Pecly, Nathalia H.
; Ferreira Júnior, Nelson
; Silva Júnior, Nelson J. da
; Perioto, Nelson W.
; Hamada, Neusa
; Degallier, Nicolas
; Chao, Ning L.
; Ferla, Noeli J.
; Mielke, Olaf H.H.
; Evangelista, Olivia
; Shibatta, Oscar A.
; Oliveira, Otto M.P.
; Albornoz, Pablo C.L.
; Dellapé, Pablo M.
; Gonçalves, Pablo R.
; Shimabukuro, Paloma H.F.
; Grossi, Paschoal
; Rodrigues, Patrícia E. da S.
; Lima, Patricia O.V.
; Velazco, Paul
; Santos, Paula B. dos
; Araújo, Paula B.
; Silva, Paula K.R.
; Riccardi, Paula R.
; Garcia, Paulo C. de A.
; Passos, Paulo G.H.
; Corgosinho, Paulo H.C.
; Lucinda, Paulo
; Costa, Paulo M.S.
; Alves, Paulo P.
; Roth, Paulo R. de O.
; Coelho, Paulo R.S.
; Duarte, Paulo R.M.
; Carvalho, Pedro F. de
; Gnaspini, Pedro
; Souza-Dias, Pedro G.B.
; Linardi, Pedro M.
; Bartholomay, Pedro R.
; Demite, Peterson R.
; Bulirsch, Petr
; Boll, Piter K.
; Pereira, Rachel M.M.
; Silva, Rafael A.P.F.
; Moura, Rafael B. de
; Boldrini, Rafael
; Silva, Rafaela A. da
; Falaschi, Rafaela L.
; Cordeiro, Ralf T.S.
; Mello, Ramon J.C.L.
; Singer, Randal A.
; Querino, Ranyse B.
; Heleodoro, Raphael A.
; Castilho, Raphael de C.
; Constantino, Reginaldo
; Guedes, Reinaldo C.
; Carrenho, Renan
; Gomes, Renata S.
; Gregorin, Renato
; Machado, Renato J.P.
; Bérnils, Renato S.
; Capellari, Renato S.
; Silva, Ricardo B.
; Kawada, Ricardo
; Dias, Ricardo M.
; Siewert, Ricardo
; Brugnera, Ricaro
; Leschen, Richard A.B.
; Constantin, Robert
; Robbins, Robert
; Pinto, Roberta R.
; Reis, Roberto E. dos
; Ramos, Robson T. da C.
; Cavichioli, Rodney R.
; Barros, Rodolfo C. de
; Caires, Rodrigo A.
; Salvador, Rodrigo B.
; Marques, Rodrigo C.
; Araújo, Rodrigo C.
; Araujo, Rodrigo de O.
; Dios, Rodrigo de V.P.
; Johnsson, Rodrigo
; Feitosa, Rodrigo M.
; Hutchings, Roger W.
; Lara, Rogéria I.R.
; Rossi, Rogério V.
; Gerstmeier, Roland
; Ochoa, Ronald
; Hutchings, Rosa S.G.
; Ale-Rocha, Rosaly
; Rocha, Rosana M. da
; Tidon, Rosana
; Brito, Rosangela
; Pellens, Roseli
; Santos, Sabrina R. dos
; Santos, Sandra D. dos
; Paiva, Sandra V.
; Santos, Sandro
; Oliveira, Sarah S. de
; Costa, Sávio C.
; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
; Bonecker, Sergio L.C.
; Bueno, Sergio L. de S.
; Almeida, Sérgio M. de
; Stampar, Sérgio N.
; Andena, Sérgio R.
; Posso, Sergio R.
; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
; Brandão, Simone N.
; Rosa, Simone P.
; Ribeiro, Síria L.B.
; Letana, Sócrates D.
; Santos, Sonia B. dos
; Andrade, Sonia C.S.
; Dávila, Stephane
; Vaz, Stéphanie
; Peck, Stewart B.
; Christo, Susete W.
; Cunha, Suzan B.Z.
; Gomes, Suzete R.
; Duarte, Tácio
; Madeira-Ott, Taís
; Marques, Taísa
; Roell, Talita
; Lima, Tarcilla C. de
; Sepulveda, Tatiana A.
; Maria, Tatiana F.
; Ruschel, Tatiana P.
; Rodrigues, Thaiana
; Marinho, Thais A.
; Almeida, Thaís M. de
; Miranda, Thaís P.
; Freitas, Thales R.O.
; Pereira, Thalles P.L.
; Zacca, Thamara
; Pacheco, Thaynara L.
; Martins, Thiago F.
; Alvarenga, Thiago M.
; Carvalho, Thiago R. de
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Henry, Thomas
; Pikart, Tiago G.
; Porto, Tiago J.
; Krolow, Tiago K.
; Carvalho, Tiago P.
; Lotufo, Tito M. da C.
; Caramaschi, Ulisses
; Pinheiro, Ulisses dos S.
; Pardiñas, Ulyses F.J.
; Maia, Valéria C.
; Tavares, Valeria
; Costa, Valmir A.
; Amaral, Vanessa S. do
; Silva, Vera C.
; Wolff, Vera R. dos S.
; Slobodian, Verônica
; Silva, Vinícius B. da
; Espíndola, Vinicius C.
; Costa-Silva, Vinicius da
; Bertaco, Vinicius de A.
; Padula, Vinícius
; Ferreira, Vinicius S.
; Silva, Vitor C.P. da
; Piacentini, Vítor de Q.
; Sandoval-Gómez, Vivian E.
; Trevine, Vivian
; Sousa, Viviane R.
; Sant’Anna, Vivianne B. de
; Mathis, Wayne N.
; Souza, Wesley de O.
; Colombo, Wesley D.
; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
4.
MULTIRESIDUE AND MULTICLASS DETERMINATION OF CURRENT-USE PESTICIDES (CUPs) IN WATER SAMPLES BY SPE AND UHPLC-MS/MS CURRENTUSE CURRENT USE CUPs (CUPs UHPLCMS/MS UHPLCMSMS UHPLC MS/MS MS UHPLCMS MSMS
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Avellar, Állisson A. da S.
; Prates, Gabriel A. B.
; Alves, Larissa da S.
; Prestes, Osmar D.
; Adaime, Martha B.
; Zanella, Renato
.
Given the extensive use of pesticides in modern times, residues are frequently detected in water samples. Therefore, this study developed and validated a method utilizing solid-phase extraction (SPE) with a polymeric sorbent for sample preparation in combination with ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The aim was to determine a variety of current-use pesticides (CUPs) in different types of water. The preconcentration step, employing the polymeric sorbent Oasis HLB (60 mg), combined with the high selectivity and sensitivity of the UHPLC-MS/MS analysis, enabled the multiclass determination of pesticide residues in water samples at very low concentrations. The proposed method underwent validation, demonstrating satisfactory linearity, precision, and accuracy. The accuracy results, determined through recovery assays at varying spike levels (0.04-0.4 μg L-1), ranged from 71 to 117%, with precision expressed as relative standard deviations consistently below 19%. The practical limits of detection and quantification for most compounds were 0.01 and 0.04 μg L-1, respectively. This method was effectively employed to analyze drinking water, artesian well water, dam water, and river water, leading to the detection of various pesticides at concentrations of up to 2.24 μg L-1. times Therefore solidphase solid phase SPE (SPE ultrahigh ultra UHPLCMS/MS. UHPLCMSMS UHPLC MS/MS . MS (UHPLC-MS/MS) currentuse current CUPs (CUPs step 60 (6 mg, mg , mg) UHPLCMS/MS analysis validation linearity results 0.040.4 00404 0.4 0 04 4 (0.04-0. L1, L1 L 1 L-1) 7 117 117% 19 19% 001 01 0.0 004 1, L-1 respectively 224 2 24 2.2 L1. 1. UHPLCMS MSMS (UHPLC-MS/MS 6 ( 040 0.040. 0040 0. (0.04-0 11 00 L- 22 2. 0.040 (0.04- (0.04 (0.0 (0. (0
5.
Stereotactic radiotherapy as a valuable therapeutic procedure for controlling aldosterone-secreting adrenocortical carcinoma aldosteronesecreting aldosterone secreting
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Mermejo, Livia Mara
; Zanella, Renato Heron
; Cocicov, Larissa
; Molina, Carlos A. Fernandes
; Tucci Junior, Silvio
; Elias Junior, Jorge
; Muglia, Valdair Francisco
; Viani, Gustavo Arruda
; Elias, Paula C L
; Moreira, Ayrton Custodio
; Castro, Margaret de
.
SUMMARY Aldosterone-secreting adrenocortical carcinomas (ACCs) are rare and usually present as large tumors. The only potentially curative treatment for ACC is surgical resection. However, surgery may be unfeasible in some patients who have multiple comorbidities or decline the procedure. We describe herein the case of a 44-year-old man with aldosterone-secreting ACC who declined surgery because of religious convictions. As a Jehovah’s Witness, the patient was concerned about requiring blood transfusion during surgery. Treatment with mitotane was started but interrupted due to hepatotoxicity. Subsequently, the patient was successfully treated with stereotactic ablative radiotherapy (SABR). After SABR, the patient had progressive improvement of pain, reduction in antihypertensive drugs, control of blood pressure and hypokalemia, normalization of serum aldosterone and renin levels, and reduction in tumor size and weight. To our knowledge, this is the first report of a patient with a pure aldosterone-secreting ACC who received SABR. The patient’s response was substantial, showing that SABR could be considered as an alternative local treatment for aldosterone-secreting ACCs when surgery is unfeasible. Aldosteronesecreting Aldosterone secreting (ACCs tumors resection However procedure 44yearold yearold 44 year old aldosteronesecreting convictions Jehovahs Jehovah s Witness hepatotoxicity Subsequently . (SABR) pain drugs hypokalemia levels weight knowledge substantial 4 (SABR
6.
MULTIRESIDUE DETERMINATION OF PESTICIDES IN EXOTIC FRUITS: A REVIEW OF THE ANALYTICAL STRATEGIES AND RESIDUE OCCURRENCE FRUITS
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Silva, Rafael Bel Prestes da
; Oliveira, Júlia Antunes de
; Zanchin, Cleusa Fátima
; Santos, Pimpernelli Jonco dos
; Prestes, Osmar Damian
; Zanella, Renato
.
The growing awareness of health and well-being has led to increased consumption of healthy foods, such as exotic fruits, owing to their high nutritional value and global availability. However, the production and trade of exotic fruit presents significant challenges, particularly with regard to increasing supply and ensuring food safety concerning pesticide residues. This review examines modern analytical strategies employed to determine pesticide residues in exotic fruits. It is evident from the literature that ultra-high performance liquid chromatography and gas chromatography techniques, coupled with tandem mass spectrometry, remain the methods of choice for determining pesticide residues. For sample preparation, the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, renowned for its simplicity and efficiency, is extensively utilized in its original form as well as in acetate, citrate, and other modified versions to enhance its effectiveness. Developing these analytical methods is crucial for ensuring the safety of exotic fruits, protecting consumer health, and facilitating exports to global markets. Further advancements in this field are essential to overcome the challenges posed by producing and trading exotic fruits, thereby promoting food safety and public health. This review also discusses the occurrence of pesticides in exotic fruits. wellbeing being foods fruits availability However ultrahigh ultra techniques spectrometry preparation Quick, Quick (Quick Easy Cheap Effective Rugged Safe method efficiency acetate citrate effectiveness markets
7.
Agrotóxicos e violações nos direitos à saúde e à soberania alimentar em comunidades Guarani Kaiowá de MS, Brasil
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Pinho, Alexandra De
; Calheiros, Débora F.
; Almeida, Fernanda S.
; Zerlotti, Patrícia
; Cereali, Mariana
; Feiden, Alberto
; Machado, Franciele F.
; Zanella, Renato
.
Resumen Brasil, uno de los mayores productores agrícolas y consumidores de plaguicidas del mundo, ha expandido su área agrícola en el sur de Mato Grosso do Sul, intensificando la contaminación ambiental y la vulnerabilidad de las poblaciones indígenas. Esta investigación evaluó la presencia de plaguicidas en las aguas de dos comunidades indígenas de MS, Retomada Guyraroká y Aldeia Jaguapiru. Entre 2021 y 2022, se realizaron tres campañas de muestreo de aguas superficiales, de abastecimiento y de lluvia, considerando el calendario agrícola. El estudio siguió el protocolo de LARP/UFSM. En total, se encontraron 22 ingredientes activos (IAs), en que, el 41% causa graves efectos en la salud y el 68% está prohibido en la Unión Europea. Fipronil, 2,4-D, y Atrazina los más frecuentes. Del total de IAs, Los resultados revelan que estas comunidades están expuestas a plaguicidas, violando sus derechos a la salud y a la soberanía alimentaria.
Resumo O Brasil, um dos maiores produtores agrícolas e consumidores de agrotóxicos do mundo, expandiu sua área agrícola no sul do Mato Grosso do Sul, intensificando a contaminação ambiental e a vulnerabilidade das populações indígenas. Esta pesquisa avaliou a presença de agrotóxicos nas águas de duas comunidades indígenas de MS, Retomada Guyraroká e Aldeia Jaguapiru. Entre 2021 e 2022 foram realizadas três campanhas de amostragem de água superficial, de abastecimento e da chuva, considerando o calendário agrícola. O estudo seguiu o protocolo do Laboratório de Análises de Resíduos de Pesticidas, da Universidade Federal de Santa Maria. Ao todo foram encontrados 22 ingredientes ativos (IAs), destes, 41% causam efeitos graves à saúde e 68% são proibidos na União Europeia. O Fipronil, 2,4-D e Atrazina, alguns dos mais frequentes. Os resultados revelam que essas comunidades estão expostas aos agrotóxicos, violando seus direitos à saúde e à soberania alimentar.
Abstract Brazil, one of the world’s largest agricultural producers and consumers of pesticides, has expanded its agricultural area in the southern region of Mato Grosso do Sul, intensifying environmental contamination and increasing the vulnerability of indigenous populations. This research assessed the presence of pesticides in the waters of two indigenous communities in MS, Retomada Guyraroká and Aldeia Jaguapiru. Between 2021 and 2022, three sampling campaigns of surface, supply, and rainwater were conducted, considering the agricultural calendar. The study followed the LARP/UFSM protocol. In total of 22 active ingredients (AIs) were found, among these, 41% cause serious health effects, and 68% are banned in the European Union. Fipronil, 2,4-D, Atrazine are the among the most frequent IA found. Results show that these communities are exposed to pesticides, violating their rights to health and food sovereignty.
8.
Equity program: strategies on clinical studies as an aggregating potential program
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Crusoe, Edvan de Queiroz
; Hallack Neto, Abrahão Elias
; Nantes, Deise Ferreira
; Ribeiro, Eduardo Flavio Oliveira
; Pinto, Fernando Monteiro Correia
; Bortolini, Jaisson Andre Pagnoncelli
; Maciel, James Farley Rafael
; Marques Junior, Jose Francisco Comenalli
; Corrêa, Lorena Costa
; Capra, Marcelo Eduardo Zanella
; Carvalho, Maria do Perpétuo Socorro Sampaio
; Melo, Nelcivone Soares de
; Quintas, Rafaela Vasques de Oliveira
; Carneiro, Thiago Xavier
; Laforga, Violete Petitto
; Cunha, Renato Luiz Guerino
.
Hematology, Transfusion and Cell Therapy
- Métricas do periódico
9.
Simultaneous Determination of Glyphosate, AMPA and Inorganic Anions in Water Samples by Gradient Capillary Ion Chromatography Glyphosate
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Matos, Fábio S. de
; Carvalho, Gabriel S. de
; Prestes, Osmar D.
; Adaime, Martha B.
; Zanella, Renato
.
Journal of the Brazilian Chemical Society
- Métricas do periódico
The herbicide glyphosate is the most widely used pesticide worldwide. Glyphosate and its major metabolite, aminomethylphosphonic acid (AMPA), have been frequently found in water samples. The widely used methods for determining these compounds are expensive and environmentally unfriendly due to reagent consumption for derivatization. Another possibility is the use of classic ion chromatography, albeit with low sensitivity and subject to interferences. Therefore, this study aimed to develop a method to directly and simultaneously determine glyphosate, AMPA, and common inorganic anions in water samples using gradient capillary ion chromatography without sample pre-treatment and derivatization. The proposed method was validated, presenting adequate linearity for glyphosate and AMPA with a determination coefficient (r2) > 0.998. Recoveries ranged from 94 to 105% and 79 to 113% for glyphosate and AMPA, respectively, with a relative standard deviation < 10%. The practical method limits of detection and quantification for both glyphosate and AMPA were 7.5 and 25 µg L 1, respectively. The method presented satisfactory results for the anions fluoride, chloride, bromide, nitrite, nitrate, phosphate, and sulfate, with limits of detection ranging from 7.5 to 200 µg L-1. Application of the method in water samples proved simple, efficient, and cost-effective, enabling the monitoring of these analytes in different water matrices. worldwide metabolite , (AMPA) derivatization interferences Therefore pretreatment pre treatment validated r2 r (r2 0998 0 998 0.998 9 105 7 113 respectively 10 10% 75 5 7. 2 1 fluoride chloride bromide nitrite nitrate phosphate sulfate 20 L1. L1 1. L-1 simple efficient costeffective, costeffective cost effective, effective cost-effective matrices (AMPA (r 099 99 0.99 11 L- 09 0.9 0.
10.
Degradation of pharmaceuticals in sanitary effluent by the combination of oxidation and photo-oxidation processes photooxidation photo
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Resumo A maioria dos sistemas convencionais de tratamento de esgoto não é capaz de remover totalmente os micropoluentes presentes no esgoto. Assim, a degradação simultânea de 11 fármacos, detectados no efluente de uma estação de tratamento de esgoto no Sul do Brasil, foi investigada por meio de processos oxidativos avançados baseados em ozonização, radiação ultravioleta e peróxido de hidrogênio. Para detecção, as amostras foram preparadas por extração em fase sólida e os fármacos foram identificados por cromatografia líquida de ultra eficiência acoplada à espectrometria de massa em tandem. Ingredientes ativos como ciprofloxacina, oxitetraciclina, paracetamol, sulfametoxazol e trimetoprima tiveram suas concentrações aumentadas para análise de degradação. Os ensaios foram realizados em bancada a temperatura ambiente e pH neutro, com alíquotas coletadas aos 7,5 e 15 minutos. Duas doses de ozônio (0,5 e 0,9 mg por mg de carbono orgânico dissolvido) e combinações da menor dose de ozônio com fotólise (254 nm) e com 25 mg.L−1 de peróxido de hidrogênio foram avaliadas. A eficiência da mineralização dos fármacos foi verificada em um analisador de carbono orgânico total. O processo combinando de ozônio, peróxido de hidrogênio e radiação ultravioleta foi o mais eficiente na degradação de todos os fármacos detectados neste estudo, pois permitiu reduzir a oxitetraciclina em 89,32%, a cafeína em 96,79%, o trimetoprim em 97,40%, a ciprofloxacina em 97,75%, o sulfametoxazol em 99,79%, o paracetamol em 99,96% e a clindamicina, a ofloxacina, a sulfadiazina, o sulfatiazol e a tilosina em 100%. Esse processo também registrou a maior taxa de mineralização (60,52%), confirmando o potencial de redução de fármacos persistentes encontrados em sistemas convencionais de tratamento de esgoto. Assim 1 Brasil ozonização detecção tandem neutro 75 7 5 7, minutos 0,5 05 0 (0, 09 9 0, dissolvido 254 (25 nm 2 mgL1 mgL L mg.L− avaliadas total estudo 8932 89 32 89,32% 9679 96 79 96,79% 9740 97 40 97,40% 9775 97,75% 9979 99 99,79% 9996 99,96 clindamicina ofloxacina sulfadiazina 100 100% 60,52%, 6052 60,52% , 60 52 (60,52%) (0 (2 mg.L 893 8 3 89,32 967 96,79 974 4 97,40 977 97,75 997 99,79 999 99,9 10 605 60,52 6 (60,52% ( 89,3 96,7 97,4 97,7 99,7 99, 60,5 (60,52 89, 96, 97, 60, (60,5 (60, (60 (6
Abstract Most conventional sewage treatment systems are not able to fully remove micropollutants found in sewage. Thus, the simultaneous degradation of 11 pharmaceuticals identified in the effluent of a sewage treatment plant in Southern Brazil was herein investigated through advanced oxidation processes based on ozonation, ultraviolet radiation and hydrogen peroxide. For detection, samples were prepared through solid-phase extraction and pharmaceuticals were identified through ultra-high performance liquid chromatography tandem mass spectrometry. Active ingredients such as ciprofloxacin, oxytetracycline, paracetamol, sulfamethoxazole and trimethoprim had their concentrations increased for degradation analysis purposes. Trials were carried out on a bench at room temperature and neutral pH, with aliquots collected at 7.5 and 15 minutes. Two ozone doses (0.5 and 0.9 mg per mg of dissolved organic carbon), and combinations of the lowest ozone dose with photolysis (254 nm) and with 25 mg.L−1 of hydrogen peroxide were evaluated. Pharmaceuticals mineralization efficiency was assessed in a total organic carbon analyzer. The process combining ozone, hydrogen peroxide and ultraviolet radiation was the most efficient in the degradation of all pharmaceuticals detected in this study, since it enabled reducing oxytetracycline by 89.32%, caffeine by 96.79%, trimethoprim by 97.40%, ciprofloxacin by 97.75%, sulfamethoxazole by 99.79%, paracetamol by 99.96%, and clindamycin, ofloxacin, sulfadiazine, sulfathiazole and tylosin by 100%. This process also recorded the highest mineralization rate (60.52%), fact that confirmed the potential to decrease persistent pharmaceuticals found in conventional sewage treatment systems. Thus 1 ozonation detection solidphase solid phase ultrahigh ultra high spectrometry purposes pH 75 7 5 7. minutes 0.5 05 0 (0. 09 9 0. carbon, , carbon) 254 (25 nm 2 mgL1 mgL L mg.L− evaluated analyzer study 8932 89 32 89.32% 9679 96 79 96.79% 9740 97 40 97.40% 9775 97.75% 9979 99 99.79% 9996 99.96% clindamycin ofloxacin sulfadiazine 100 100% 60.52%, 6052 60.52% 60 52 (60.52%) (0 (2 mg.L 893 8 3 89.32 967 96.79 974 4 97.40 977 97.75 997 99.79 999 99.96 10 605 60.52 6 (60.52% ( 89.3 96.7 97.4 97.7 99.7 99.9 60.5 (60.52 89. 96. 97. 99. 60. (60.5 (60. (60 (6
11.
Fatty acid profile, omegas and lipid quality in commercial cuts of pirarucu (Arapaima gigas Schinz, 1822) cultivated in excavated tanks profile Arapaima Schinz 1822 182 18 1
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Cavali, Jucilene
; Dantas Filho, Jerônimo Vieira
; Nunes, Carla Taveira
; Ferreira, Elvino
; Pontuschka, Rute Bianchini
; Zanella, Renato
; Souza, Maria Luiza Rodrigues de
.
ABSTRACT. The study aimed to determine the fatty acid profile, omegas and lipid quality indeces in commercial cuts of pirarucu (Arapaima gigas) of the slaughter class 11.1 to 14.0 kg. Sample collections were carried out in two fish processing industries located in Rondônia state, Brazil. The experimental design was completely randomized, with processing performed in triplicate. Data were submitted to ANOVA to assess differences between commercial cuts in chemical compositions. If ANOVA appeared statistically significant (α=0.05), the averages were compared by Tukey's test. In the composition of fatty acids, there was a difference (p <0.05) between cuts. Commercial cuts that expressed the highest percentages of SFAs tail fillet 51.2%, of MUFAs fillet mignon 39.8% and of PUFAs deboned cut 20.7%. The indeces prescribed for lipid quality, ∑PUFAs/∑SFAs, ∑PUFAs (n-6/∑n-3), AI, TI and HH, indicate that commercial cuts have lipid quality. Deboned is the cut with the highest PUFA fatty acid content, with the highest values of Omega 3, 6, 7 and n-9 being also expressed. Nutritional information is important for the processes of conservation and processing, development of new products on the market, as well as guiding the form of preparation, thus providing commercial security for different market niches. ABSTRACT profile Arapaima gigas 111 11 1 11. 140 14 0 14. kg state Brazil randomized triplicate compositions α=0.05, α005 α α=0.05 , 05 (α=0.05) Tukeys Tukey s test acids p <0.05 005 512 51 2 51.2% 398 39 8 39.8 207 20 20.7% PUFAsSFAs ∑PUFAs/∑SFAs n6/∑n3, n6n3 nn n 6/∑n 3 6 (n-6/∑n-3) AI HH content n9 9 n- preparation niches α00 α=0.0 (α=0.05 <0.0 00 5 51.2 39. 20.7 n6 n3 n6/∑n3 n6n 6n (n-6/∑n-3 α0 α=0. (α=0.0 <0. 51. 20. n6/∑n (n-6/∑n- α=0 (α=0. <0 (n-6/∑n α= (α=0 < (α= (α
12.
Influence of adjuvants added to teflubenzuron spray on resistant and susceptible strains of the soybean looper Chrysodeixis includens (Lepidoptera: Noctuidae) Lepidoptera (Lepidoptera Noctuidae
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Hettwer, Bruna Laise
; Godoy, Daniela Neves
; Hanich, Manoela Ramos
; Caye, Milena
; Moreira, Rafaella Pretto
; Luchese, Eric Fernandes
; Zanella, Renato
; Bernardi, Oderlei
; Melo, Adriano Arrué
.
RESUMO: A lagarta falsa-medideira, Chrysodeixis includens (Walker, [1858]) (Lepidoptera: Noctuidae), é uma praga da soja e do algodão nos países da América do Sul. A resistência de C. includens a inibidores da biossíntese de quitina tem sido relatada no Brasil. Entretanto, esse modo de ação ainda é amplamente utilizado para controle de C. includens. Com base nisso, conduzimos bioensaios em laboratório para investigar se adjuvantes (Nimbus®, TA 35®, Break-Thru® S 240 e Rizospray Extremo®) adicionados à calda inseticida de teflubenzuron aumentam a mortalidade de linhagens de C. includens (resistentes, heterozigotos e suscetíveis a inibidores da biossíntese de quitina). Usando análise cromatográfica, também avaliamos a quantidade de teflubenzuron em folhas de soja quando aplicado isolado ou em combinação com adjuvantes. Em bioensaios de laboratório, a atividade biológica do teflubenzuron aumentou para a linhagem suscetível quando os adjuvantes foram adicionados à calda inseticida. Em contraste, nenhum efeito relevante de adjuvantes adicionados ao teflubenzuron foi detectado para os heterozigotos e resistentes. Em bioensaios de folhas, mesmo naquelas do terço superior das plantas, as quais apresentaram uma maior deposição de teflubenzuron (3,4 mg/kg vs 1,7 e 0,6 mg/kg); a mortalidade das linhagens de C. includens foi semelhante quando o teflubenzuron foi aplicado isolado ou com adjuvantes. Nossos resultados indicam que os adjuvantes adicionados ao teflubenzuron não fornecem um aumento substancial na mortalidade de linhagens de C. includens resistentes aos inibidores da biossíntese de quitina. Portanto, é necessário reduzir o uso desse modo de ação para o manejo de C. includens e dar preferência a outros inseticidas ou tática de controle. RESUMO falsamedideira, falsamedideira falsa medideira, medideira falsa-medideira Walker, Walker (Walker 1858 [1858] Lepidoptera (Lepidoptera Noctuidae, Noctuidae , Noctuidae) Sul C Brasil Entretanto nisso Nimbus®, Nimbus (Nimbus® 35 35® BreakThru® BreakThru Break Thru® Thru Break-Thru 24 Extremo® Extremo resistentes, (resistentes . quitina) cromatográfica contraste plantas 3,4 34 3 4 (3, mgkg mg kg 17 1 7 1, 06 0 6 0, mg/kg) Portanto 185 [1858 Nimbus® (Nimbus 2 3, (3 18 [185 ( [18 [1 [
ABSTRACT: The soybean looper (SBL), Chrysodeixis includens (Walker, [1858]) (Lepidoptera: Noctuidae), is a soybean and cotton pest in South America countries. Field-evolved resistance of SBL to inhibitors of chitin biosynthesis has been reported in Brazil; however, this mode of action is still widely used against SBL. On this basis, we conducted laboratory bioassays to investigate if adjuvants (Nimbus®, TA 35®, Break-Thru® S 240, and Rizospray Extremo®) added to the teflubenzuron spray increase the mortality of SBL strains (resistant, heterozygous, and susceptible to chitin biosynthesis inhibitors). Using chromatography analysis, we also evaluated the amount of teflubenzuron on soybean leaves when applied alone or in combination with adjuvants. In laboratory bioassays, the biological activity of teflubenzuron increased against the susceptible SBL strain when adjuvants were added. In contrast, no relevant effects of adjuvants added to the teflubenzuron spray against heterozygous and resistant SBL larvae were detected. In leaf bioassays, even leaves from the upper third part of the plants containing a significantly higher amount of teflubenzuron (3.4 mg/kg vs 1.7 and 0.6 mg/kg); the mortality of SBL strains was similar when teflubenzuron was applied alone or in mixture with adjuvants. Our findings indicated that adjuvants added to teflubenzuron spray do not provide a substantial increase in the mortality of SBL strains resistant to chitin biosynthesis inhibitors. Therefore, it is necessary to reduce the use of this mode-of-action insecticide against SBL and to give preference to other insecticides or control tactic. ABSTRACT SBL, , (SBL) Walker, Walker (Walker 1858 [1858] Lepidoptera (Lepidoptera Noctuidae, Noctuidae Noctuidae) countries Fieldevolved Field evolved Brazil however basis Nimbus®, Nimbus (Nimbus® 35 35® BreakThru® BreakThru Break Thru® Thru Break-Thru 240 Extremo® Extremo resistant, (resistant . inhibitors) analysis contrast detected 3.4 34 3 4 (3. mgkg mg kg 17 1 7 1. 06 0 6 0. mg/kg) Therefore modeofaction tactic (SBL 185 [1858 Nimbus® (Nimbus 24 3. (3 18 [185 2 ( [18 [1 [
13.
Simultaneous GC-NCI-MS Determination of Persistent Organic Pollutants and Current-Use Pesticides in Breast Milk Samples
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Viera, Mariela S.
; Ferronato, Giovana
; Abreu, Herliana D. F.
; Prestes, Osmar D.
; Adaime, Martha B.
; Zanella, Renato
.
Journal of the Brazilian Chemical Society
- Métricas do periódico
To preserve human health, persistent organic pollutants (POPs) and current-use pesticides (CUPs) should be monitored in fatty tissues, including breast milk. Therefore, this study aimed to optimize sample preparation conditions using a 32 factorial design for the determination of POPs and CUPs by gas chromatography coupled to mass spectrometry with negative chemical ionization (GC-NCI-MS). The method was validated for 57 POP and CUP compounds using hexane:acetone for extraction and clean-up by dispersive solid phase extraction (d-SPE) with Florisil®. The matrix effect was compensated by extracted analytical calibration. Method validation showed satisfactory results with limits of detection of 3 to 13 ng g-1 of fat. The method presented adequate accuracy (recoveries from 72 to 117%) and precision (relative standard deviation (RSD) ≤ 18%) and was applied to breast milk samples from Rio Grande do Sul State, southern Brazil, wherein all samples contained at least one compound. With principal component analysis, it was possible to associate the pesticides detected with the city of origin of the samples and the number of pregnancies of nursing mothers. Additionally, the analytical method was effective for the determination of trace levels of POPs and CUPs in breast milk and can be applied in biomonitoring studies.
14.
Do phytoplankton and epiphyton communities differ between organic and conventional rice fields?
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ABSTRACT This study aimed to determine if there are any differences in the attributes and composition of the phytoplankton and epiphyton communities between organic (OF) and conventional (CF) rice fields. We also strove to identify if there were any variations in these communities by comparing samples taken from two different periods (12 and 35 days) after the application of the herbicide clomazone and penoxsulam in CF. The farms are located in the Pampa Biome, Southern Brazil. Phytoplankton samples from the subsurface water and epiphyton samples from the rice stems were analyzed using the Utermöhl method. The CF and OF had distinct environmental conditions (pH, conductivity, and turbidity values), and the residual concentration of the herbicides decreased over time. There were no significant differences in epiphyton and phytoplankton density, or in phytoplankton richness, between the rice fields; only the epiphyton richness and taxonomic composition showed differences between the rice fields. Cyanobacteria and Chlorophyceae comprised a large proportion of the epiphytic density in CF and OF, respectively. However, Bacillariophyceae and Chlorophyceae had greater phytoplankton densities in CF and OF, respectively. The taxonomic composition of communities should be considered an effective tool to show the differences between the two cultivation systems.
15.
Experimental reproduction of congenital anomalies in the progeny of cows fed apple pomace during pregnancy
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Wicpolt, Nathalia S.
; Morais, Raissa M.
; Molossi, Francieli Adriane
; Ogliari, Daiane
; Mezzalira, Joana
; Prestes, Osmar D.
; Zanella, Renato
; Gava, Aldo
.
RESUMO: O presente trabalho tem por finalidade descrever e discutir os resultados do experimento realizado em vacas prenhes que foram alimentadas com 25kg/dia de bagaço de maçã. Experimentos foram conduzidos em duas etapas, a primeira no ano de 2015, de setembro a dezembro onde foram utilizadas 16 vacas prenhes da raça holandês. Estas foram divididas em quatro grupos: Grupo 0, Controle (5 vacas); Grupo I, 1 mês gestação (4 vacas); Grupo II, 3 meses gestação (4 vacas); Grupo III, 6 meses gestação (3 vacas). A segunda etapa foi realizada em abril de 2016 a fevereiro de 2017. Foram utilizadas 12 vacas prenhes da raça holandês, divididas em três grupos: Grupo 0, Controle (6 vacas); Grupo I, 1 mês gestação (3 vacas); Grupo II, 3 meses gestação (3 vacas). Todas as vacas receberam bagaço de maçã na dose de 25kg/dia/vaca. Para o primeiro experimento, uma vaca do Grupo III pariu uma bezerra, com ausência completa das vértebras coccígeas e cauda, encurvamento leve dos membros posteriores, escoliose na coluna torácica e dificuldade de locomoção. Decorridos 30 dias do nascimento, manifestou diarreia e pouco desenvolvimento, sendo eutanasiada para necropsia. Na macroscopia, havia malformações na coluna torácica, articulação coxofemoral e no aparelho urogenital. Em relação ao segundo experimento uma vaca do Grupo I pariu uma bezerra com membros pélvicos e torácicos, curvos e com articulações consideravelmente grossas e “achinelamento” de cascos. Na microscopia do fêmur foi observado na placa epifisária, zona hipertrófica desorganizada, irregular e zona de crescimento escassa. Na zona esponjosa primária observou-se trabéculas curtas e pouco mineralizadas. Amostras do bagaço de maçã utilizado na experimentação foram congeladas e enviadas para avaliação de resíduos agrotóxicos, onde foi encontrado resultado positivo para o fungicida carbendazim.
ABSTRACT: This study aimed to describe and discuss the results of an experiment carried out in two stages with pregnant cows fed 25kg/apple pomace/day. The first stage involved 16 pregnant Holstein Friesian cows divided into four groups: Group 0 - Control (5 cows); Group I - 1 month-gestation (4 cows); Group II - 3 month-gestation (4 cows); Group III - 6 month-gestation (3 cows) and was performed from September to December 2015. The second stage comprised 12 pregnant Holstein Friesian cows divided into three groups: Group 0 - Control (6 cows), Group I - 1 month-gestation (3 cows), and Group II - 3 month-gestation (3 cows) and was conducted from April 2016 to February 2017. All study animals received apple pomace at a dose of 25kg/day. As for the first experiment stage, a cow in Group III bred a calf with complete absence of the coccygeal vertebrae and tail, slight bending of the hind limbs, scoliosis in the thoracic spine, and limited mobility. At 30 days, it presented with diarrhea and underdevelopment, and was euthanized for necropsy. At gross examination, malformations were observed in the thoracic spine, coxofemural joint, and genitourinary tract. Regarding the second experiment stage, a cow in Group I gave birth to a calf with curved pelvic and thoracic limbs with thick joints and flattening hooves. Microscopic examination of the femur showed disorganized, irregular hypertrophic zone and scarce growth zone, in addition to primary spongy zone with short, slightly mineralized trabeculae. Samples of the apple pomace used in this study were frozen and sent for laboratory evaluation of pesticide residues, which showed a positive result for the fungicide carbendazim.
https://doi.org/10.1590/1678-5150-pvb-6119
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