Abstract Background Rheumatoid arthritis (RA) is a chronic autoimmune disease that may cause joint deformities and seriously affect the normal life of the patients. In order to enable patients to receive timely attention and treatment, this study developed new diagnostic markers by exploring the expression and molecular mechanism of the long non-coding RNA NORAD (NORAD) in RA. Methods Participants including 77 RA patients and 52 healthy persons were enrolled, and the corresponding clinical data and serum samples were obtained. The NORAD and miR-204-5p expression were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). The content of inflammatory cytokines (IL-6, TNF-α) were determined through enzyme-linked immunosorbent assay (ELISA). Luciferase activity reporter assay demonstrated the association between NORAD and miR-204-5p. In addition, receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of NORAD, and Pearson's correlation analysis was applied for the correlation analysis. Results NORAD was enriched in RA serum with high diagnostic value. Simultaneously, IL-6 and TNF-α levels were also upregulated (P < 0.001). The C-reactive protein (CRP), rheumatoid factor (RF), erythrocyte sedimentation rate (ESR) and anti-cyclic citrullinated peptide antibody (Anti-CCP) levels in RA patients were generally elevated (P < 0.001). NORAD was positively correlated with the levels of clinical indicators and inflammatory factors (P < 0.0001). Mechanistically, NORAD may affect the progression of RA by targeting and negatively regulating miR-204-5p. Conclusions There is a correlation between NORAD and the processes of RA, and NORAD has the potential to predict and diagnose the occurrence of RA. (RA treatment noncoding non coding (NORAD 7 5 enrolled obtained miR2045p miRp miR 204 5p p realtime real time RTqPCR. RTqPCR RT qPCR . (RT-qPCR) IL6, IL6 IL 6, 6 (IL-6 TNFα TNF α enzymelinked enzyme linked ELISA. ELISA (ELISA) miR2045p. 5p. addition ROC (ROC Pearsons Pearson s value Simultaneously IL- P 0.001. 0001 0.001 0 001 0.001) Creactive C reactive CRP, CRP , (CRP) RF, RF (RF) ESR (ESR anticyclic anti cyclic AntiCCP Anti CCP (Anti-CCP 0.0001. 00001 0.0001 0.0001) Mechanistically 20 (RT-qPCR (IL- (ELISA 000 0.00 00 (CRP (RF 0000 0.000 2 (IL 0.0 0.

ABSTRACT Through a long-term micro-area positioning experiment (2006-2017), the precise fertilization locations of spring maize (Zea mays L.) in Jilin, China, were investigated using an embedded cement bucket pot. The effects of different fertilization placements and additional application of organic manure on maize yield, biomass, grain nutrient uptake, and soil fertility were examined. Six treatments were designed: base fertilizer + topdressing shallow application (BF1TD1); base fertilizer deep + topdressing shallow application (BF2TD1); base fertilizer + topdressing deep application (BF1TD2); base fertilizer deep + topdressing deep application (BF2TD2); base fertilizer + topdressing + organic manure shallow application (BF1TD1 + OS); and base fertilizer + topdressing shallow application + organic manure deep application (BF1TD1 + OD). After 12 years of continuous experimentation, the findings revealed that BF2TD2, BF1TD1 + OS, and BF1TD1 + OD exhibited 10.59 %, 25.17 %, and 29.34 % higher average yields than BF1TD1, respectively. Deep topdressing was more beneficial in increasing maize yield and nutrient accumulation, and additionally, increasing the use of organic fertilizer enhanced plant biomass and nutrient uptake. Over the 12 years considered, the soil organic matter in the 0-20 cm and 20-40 cm layers increased by 45.96 % and 80.61 %, respectively, and the soil pH increased from 0.38 to 0.48. In general, the deep application of organic manure was more beneficial for soil retention as it can increase soil fertility in the 0-40 cm layer. Considering the high yield and nutrient absorption and utilization of maize, technical advancement for soil fertilizer in whole-field tillage layers was crucial. longterm long term microarea micro area 20062017, 20062017 2006 2017 , (2006-2017) Zea L. L Jilin China pot uptake examined designed BFTD BF TD (BF1TD1) BF2TD1 (BF2TD1) BF1TD2 (BF1TD2) BF2TD2 (BF2TD2) (BF1TD OS OS) OD. . OD) 1 experimentation BF1TD 1059 10 59 10.5 2517 25 17 25.1 2934 29 34 29.3 respectively accumulation additionally considered 020 0 20 0-2 2040 40 20-4 4596 45 96 45.9 8061 80 61 80.6 038 38 0.3 048 48 0.48 general 040 0-4 layer wholefield whole field crucial 2006201 200 201 (2006-2017 BF2TD (BF2TD1 (BF1TD2 (BF2TD2 105 5 10. 251 2 25. 293 3 29. 02 0- 204 4 20- 459 9 45. 806 8 6 80. 03 0. 04 0.4 200620 (2006-201 (BF2TD 20062 (2006-20 (2006-2 (2006- (2006 (200 (20 (2 (

Abstract Introduction: Propofol is a widely used anesthetic and its dose is closely related to aging. Telomere length (TL) is a unique heritable trait, and emerging as a biomarker of aging, health and disease. Telomerase RNA component (TERC) plays an important role in maintaining TL. We proposed a hypothesis that propofol dose in general anesthesia can be predicted by measuring TL before operation, which greatly reduced the risk of anesthesia, especially the elderly. Methods: The association between the propofol dose in anesthesia induction and: TL in the DNA of peripheral blood leukocytes; body weight; sex; difference of the Bispectral Index (BIS) before and after anesthesia induction in patients was evaluated by multivariable linear regression analyses. The mutation at the 5’end or 3’end of TERC was detected. We recruited 100 patients of elective surgery. Results: We found that propofol dose in anesthesia induction was clearly correlated significantly with TL (r = 0.78, p < 0.001), body weight (r = 0.84, p = 0.004), sex (r = 0.83, p= 0.84, p = 0.004), sex (r = 0.83, p = 0.004), and difference of BIS before and after anesthesia induction (r = 0.85, p = 0.029). By comparing the absolute values of standardized regression coefficients (0.58, 0.21, 0.19, and 0.12) of the four variables, it can be seen that TL contributes the most to the propofol dose in anesthesia induction. However, the mutation at the 5’ end or 3’ end of TERC was not found. Conclusions: These findings provide preliminary evidence that the propofol dose in anesthesia induction was clearly correlated with genetically determined TL. TL may be a promising predictor of the propofol dose, which is beneficial to improve the safety of anesthesia and reduce perioperative complications. Introduction aging (TL trait disease (TERC operation elderly Methods leukocytes (BIS analyses 5end 5 3end 3 detected 10 surgery Results r 078 0 78 0.78 0.001, 0001 0.001 , 001 0.001) 084 84 0.84 0.004, 0004 0.004 004 0.004) 083 83 0.83 085 85 0.85 0.029. 0029 0.029 . 029 0.029) 0.58, 058 58 (0.58 021 21 0.21 019 19 0.19 0.12 012 12 variables However Conclusions complications 1 07 7 0.7 000 0.00 00 08 8 0.8 002 0.02 02 0.58 05 (0.5 2 0.2 01 0.1 0. 0.0 0.5 (0. (0 (

Abstract Objective: to explore the association between serum vitamin D level and the occurrence and pathological grade of gastric cancer. Material and methods: search PubMed, Embase, Web of Science, Cochrane and Chinese database; all articles about the association between serum vitamin D levels and gastric cancer published before July 2021. Results: 10 trials with 1159 cases of gastric cancer patients and 33,387 cases of regular control patients were analyzed. The serum vitamin D level of the gastric cancer group (15.56 ± 7.46 ng/ml) was lower than in the control group (17.60 ± 1.61 ng/ml), and the difference was statistically significant. The patients with gastric cancer, clinical stage III/IV (16.19 ± 8.04 ng/ml) had lower vitamin D levels than those with stage I/II (19.61 ± 9.61 ng/ml), and the patients with low differentiation of gastric cancer (17.5 ± 9.5 ng/ml) had lower levels than those with well- or moderately-differentiated cancer (18.04 ± 7.92 ng/ml). The patients with lymph node metastasis (19.41 ± 8.63 ng/ml) had lower vitamin D levels than the patients without lymph node metastasis (20.65 ± 7.96 ng/ml), and the difference was statistically significant. Conclusion: vitamin D levels were negatively associated with gastric cancer. Vitamin D levels were significantly associated with different clinical stages, degrees of differentiation, and lymph node metastasis, suggesting that low vitamin D levels might predict poor prognosis in gastric cancer.

Resumen Objetivo: investigar la asociación entre los niveles de vitamina D en suero y la carcinogénesis gástrica y su clasificación patológica. Material y métodos: se buscaron en las bases de datos PubMed, Embase, Web of Science, Cochrane y China todos los artículos sobre la asociación entre los niveles séricos de vitamina D y el cáncer gástrico publicados antes de julio de 2021. Resultados: se analizaron diez datos de 1159 pacientes con cáncer gástrico y 33.387 pacientes normales de control. El nivel de vitamina D en suero del grupo (15,56 ± 7,46 ng/ml) de cáncer gástrico era inferior al del grupo de control (16,19 ± 8,04 ng/ml), y la diferencia era estadísticamente significativa. En los pacientes con cáncer gástrico en estadio clínico III/IV (16,19 ± 8,04 ng/ml) era inferior al de los pacientes en estadio I/II (19,61 ± 9,61 ng/ml) y en los pacientes con cáncer de estómago poco diferenciado (17,5 ± 9,5 ng/ml) era menor que en los pacientes con cáncer bien o moderadamente diferenciado (18,04 ± 7,92 ng/ml); en los pacientes con metástasis en ganglios linfáticos (19,41 ± 8,63 ng/ml) era inferior al de los pacientes sin metástasis en ganglios linfáticos (20,65 ± 7,96 ng/ml), siendo la diferencia estadísticamente significativa. Conclusión: los niveles de vitamina D se correlacionaron negativamente con el cáncer gástrico. Los niveles de vitamina D se asociaron significativamente a los diferentes estadios clínicos, el grado de diferenciación y la metástasis en ganglios linfáticos, lo que sugiere que los niveles bajos de vitamina D pueden ser un factor de predicción de mal pronóstico en el cáncer gástrico.

OBJECTIVE: The objective of this study was to examine whether the National Institute of Health Stroke Scale was associated with the short- and long-term prognosis of patients with acute ischemic stroke treated with intravenous thrombolysis. METHODS: A total of 247 patients with acute ischemic stroke admitted to the hospital from April 2019 to October 2020 were retrospectively selected as study subjects, and the immediate and long-term prognosis after thrombolysis was assessed using the modified Rankin Scale and divided into good prognosis group (119 cases) and poor prognosis group (128 cases) based on the effect of thrombolysis. Both groups were treated with alteplase, the National Institute of Health Stroke Scale of the two groups was compared, and the factors affecting the prognosis of acute ischemic stroke were analyzed. RESULTS: After intravenous thrombolysis, 24 h, and 7 days of treatment, the National Institute of Health Stroke Scale in the poor prognosis group was higher than those of patients in the good prognosis group, and the differences were statistically significant (p<0.05). The results of the multivariate analysis suggested that National Institute of Health Stroke Scale before treatment was an independent factor associated with the 3-month (OR: 1.068, 95%CI 1.015–1.123, p=0.011) and long-term poor prognosis (OR: 1.064, 95%CI 1.012–1.119, p=0.015) in patients with acute ischemic stroke receiving intravenous thrombolysis after adjustment of age, gender, body mass index, smoking, alcohol consumer, onset-to-door time, door-to-needle time, and imaging score. CONCLUSION: The National Institute of Health Stroke Scale could be a promising indicator for the prognosis, and active intervention is needed to improve the quality of life in patients with acute ischemic stroke. OBJECTIVE short longterm long term METHODS 201 202 subjects 119 (11 cases 128 (12 alteplase compared analyzed RESULTS 2 h p<0.05. p005 p p<0.05 . 0 05 (p<0.05) 3month month 3 OR (OR 1068 1 068 1.068 95CI CI 95 10151123 015 123 1.015–1.123 p=0.011 p0011 011 1064 064 1.064 10121119 012 1.012–1.119 p=0.015 p0015 age gender index smoking consumer onsettodoor onset door time doortoneedle needle score CONCLUSION 20 11 (1 12 p00 p<0.0 (p<0.05 106 06 1.06 9 1015112 01 1.015–1.12 p=0.01 p001 1012111 1.012–1.11 ( p0 p<0. (p<0.0 10 1.0 101511 1.015–1.1 p=0.0 101211 1.012–1.1 p<0 (p<0. 1. 10151 1.015–1. p=0. 10121 1.012–1. p< (p<0 1015 1.015–1 p=0 1012 1.012–1 (p< 101 1.015– p= 1.012– (p 1.015 1.012 1.01

Abstract This study aimed to explore the effect of aged pork fat on the quality and volatile compounds of Chi-aroma Baijiu during the ageing stage. Result showed that glycerol and total ester contents of CBN, CB1, CB3 and CB5 were significantly higher than that in BL (P < 0.05), but total acid contents did not change significantly (P > 0.05). Moreover, there was a difference in volatile compounds concentration and OAVs among the Chi-aroma Baijiu samples. With the year of aged pork fat increased, concentrations of esters and aldehydes were increased and then decreased, which reached highest in CB1. CB1 had highest OAVs of medium and long-chain ethyl esters and four aldehydes ((E)-2-nonenal, octanal, (E)-2-decanal, (E)-2-octenal) were the characteristic volatile compounds of Chi-aroma Baijiu during ageing process owing to their OAV > 10. Therefore, CB1 had the highest sensory score, with intense Chi-aroma and pleasant aftertaste. OPLS-DA further effectively distinguish different Chi-aroma Baijiu soaking with different years of aged pork fat, and confirmed 31volatile compounds (especial ethyl esters) were the key volatile compounds responsible for significant differences in Chi-aroma Baijiu. Combined with the results of sensory evaluation, GC-MS and OAVs, one-year aged pork fat was the best to product Chi-aroma Baijiu. Chiaroma Chi aroma stage CBN CB P 0.05, 005 0.05 , 0 05 0.05) 0.05. . Moreover samples decreased longchain long chain E2nonenal, E2nonenal Enonenal E 2 nonenal, nonenal ((E)-2-nonenal octanal E2decanal, E2decanal Edecanal decanal, decanal (E)-2-decanal E2octenal Eoctenal octenal (E)-2-octenal 10 Therefore score aftertaste OPLSDA OPLS DA especial evaluation GCMS GC MS oneyear one 00 0.0 1 0.

RESUMEN Introducción: La investigación sobre el entrenamiento con vibraciones ha experimentado un periodo de desarrollo aplicándose en muchos proyectos como el bádminton, el balonmano, el salto de longitud y el voleibol. Sin embargo, todavía no hay una evaluación cuantitativa de la investigación sobre sus efectos en el hombro, el codo y en el desarrollo de la fuerza muscular en las extremidades superiores de los atletas de voleibol. Se cree que un protocolo de entrenamiento específico con vibración puede aportar beneficios al rendimiento sensomotor y a la implementación de la fuerza muscular en los atletas de voleibol. Objetivo: Estudiar los efectos del entrenamiento con vibraciones sobre la función de las extremidades superiores en jugadores de voleibol. Métodos: Se utilizaron métodos de investigación literarios, experimentales y estadísticos matemáticos para explorar la relación entre el entrenamiento con vibración bajo la fuerza muscular de los miembros superiores y sus articulaciones. Resultados: El entrenamiento vibratorio con amplitud de 2mm, en una frecuencia de vibración entre 30Hz y 45Hz, la frecuencia del entrenamiento vibratorio se presentó inversamente proporcional al efecto del entrenamiento vibratorio. Conclusión: El entrenamiento con vibraciones mostró beneficios de coordinación motora y aumento de la fuerza muscular en jugadores de voleibol. Una estrategia adecuada de entrenamiento con vibraciones puede maximizar las habilidades de los atletas, como la coordinación corporal, la flexibilidad y la capacidad de salto. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción bádminton balonmano voleibol embargo hombro Objetivo Métodos literarios articulaciones Resultados 2mm mm Hz 45Hz Conclusión corporal II tratamiento

ABSTRACT Introduction: The research on vibration training has experienced a period of development in many projects, such as badminton, handball, long jump, and volleyball. However, there is still no quantitative research evaluation of its effects on the development of shoulder, elbow, and upper limb muscle strength in volleyball athletes. It is believed that a specific training protocol with vibration may bring benefits to sensory-motor performance and muscle strength implementation in volleyball athletes. Objective: To study the effects of vibration training on upper limb function in volleyball players. Methods: Literature, experimental, and mathematical-statistical research methods were used to explore the relationship between vibration training under the muscle strength of the upper limbs and their joints. Results: The vibration training with an amplitude of 2mm, at a vibration frequency between 30Hz and 45Hz, the frequency of vibration training presented inversely proportional to the effect of vibration training. Conclusion: Vibration training showed the benefits of motor coordination and increased muscle strength in volleyball players. An appropriate vibration training strategy can maximize athletes’ skills, such as body coordination, flexibility, and jumping ability. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction projects badminton handball jump However shoulder elbow athletes sensorymotor sensory Objective players Methods Literature experimental mathematicalstatistical mathematical statistical joints Results 2mm mm Hz 45Hz Conclusion skills flexibility ability II outcomes

RESUMO Introdução: A pesquisa sobre o treinamento por vibração experimentou um período de desenvolvimento sendo aplicada em muitos projetos como badminton, handebol, salto em distância e voleibol. Entretanto, ainda não há uma avaliação quantitativa da pesquisa sobre seus efeitos em ombro, cotovelo e sobre o desenvolvimento de força muscular nos membros superiores dos atletas de voleibol. Acredita-se que um protocolo de treino específico com vibração possa trazer benefícios ao desempenho sensório-motor e implementação de força muscular nos atletas de voleibol. Objetivo: Estudar os efeitos do treinamento por vibração sobre a função dos membros superiores dos jogadores de vôlei. Métodos: Utilizou-se métodos de pesquisa bibliográfica, experimental e estatística matemática para explorar a relação entre o treinamento vibratório sob a força muscular dos membros superiores e suas articulações. Resultados: O treinamento vibratório com amplitude de 2mm, numa frequência de vibração entre 30Hz e 45Hz, a frequência do treinamento vibratório apresentou-se inversamente proporcional ao efeito do treinamento vibratório. Conclusão: O treinamento vibratório mostrou benefícios de coordenação motora e aumento de força muscular nos jogadores de voleibol. Uma estratégia adequada de treinamento por vibração pode maximizar as habilidades dos atletas, tais como coordenação corporal, flexibilidade e habilidade de salto. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução badminton handebol voleibol Entretanto ombro Acreditase Acredita se sensóriomotor sensório motor Objetivo vôlei Métodos Utilizouse Utilizou bibliográfica articulações Resultados 2mm mm Hz 45Hz apresentouse apresentou Conclusão corporal II tratamento

RESUMEN Introducción: Las lesiones siempre acompañarán a un deportista a lo largo de su carrera. Al analizar los factores que causan las lesiones deportivas, los entrenadores suelen tener en cuenta los factores ambientales y de entrenamiento físico, ignorando los factores psicológicos. Objetivo: Investigar el estado de salud mental de los atletas universitarios después de sufrir lesiones graves durante las competiciones, analizando el impacto de las lesiones deportivas en la salud mental de los atletas. Métodos: Se seleccionaron doce atletas universitarios como voluntarios para la investigación. Se dividieron en dos grupos de forma aleatoria. El grupo de control recibió una psicoterapia tradicional, mientras que el grupo experimental recibió una intervención mental postraumática. Se realizó una comparación y se analizó principalmente la situación general de las lesiones deportivas y el efecto del tratamiento de intervención. También se estudió el impacto de los métodos de intervención psicológica en la salud mental de los deportistas después de las lesiones, utilizando el método de la estadística matemática para analizar los datos. Resultados: Hubo diferencias significativas en las puntuaciones del STAI en el grupo experimental antes y después de la intervención. Las puntuaciones del BFS en el grupo experimental fueron significativamente más altas que las del grupo de control antes y después de la intervención. No hubo diferencias significativas en los indicadores del grupo de control. Conclusión: La intervención psicológica postraumática tiene un efecto positivo en la recuperación de la salud mental de los deportistas universitarios. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción carrera físico psicológicos Objetivo competiciones Métodos aleatoria tradicional datos Resultados Conclusión II

ABSTRACT Introduction: Injuries will always be with an athlete throughout their career. When analyzing the factors that cause athletic injuries, their coaches usually consider environmental and physical training factors, ignoring psychological factors. Objective: Investigate the mental health status of college athletes after serious injuries during competition, discussing the impact of sports injuries on athletes' mental health. Methods: Twelve college athletes were selected as research volunteers. They were divided into two groups by randomization. The control group was followed by traditional psychotherapy while the experimental group received the post-traumatic mental intervention. A comparison was carried out and mainly the general situation of sports injuries and the effect of intervention treatment were analyzed. The impact of psychological intervention methods on athletes' mental health after injuries were also studied using mathematical statistics to analyze the data. Results: There were significant differences in STAI scores in the experimental group before and after the intervention. BFS scores in the experimental group were significantly higher than those in the control group before and after an intervention. There was no significant difference in the indicators in the control group. Conclusion: Post-traumatic psychological intervention positively affects the mental health recovery of college athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction career Objective competition Methods volunteers randomization posttraumatic post traumatic analyzed data Results Conclusion Posttraumatic Post II outcomes

RESUMO Introdução: Os ferimentos estarão sempre com um atleta durante toda a sua carreira. Ao analisar os fatores que causam as lesões atléticas, seus treinadores geralmente consideram fatores ambientais e de treinamento físico, ignorando os fatores psicológicos. Objetivo: Investigar o estado de saúde mental dos atletas universitários após as lesões graves durante as competições, discutindo o impacto das lesões esportivas sobre a saúde mental dos atletas. Métodos: Foram selecionados 12 atletas universitários como voluntários de pesquisa. Eles foram divididos em dois grupos por randomização. O grupo de controle foi acompanhado pela psicoterapia tradicional enquanto o grupo experimental recebeu intervenção mental pós-traumática. Foi efetuada a comparação e analisada principalmente a situação geral das lesões esportivas e o efeito do tratamento de intervenção. O impacto dos métodos de intervenção psicológica sobre a saúde mental dos atletas após lesões também foi estudado utilizando o método de estatística matemática que é usado para analisar os dados. Resultados: Houve diferenças significativas na pontuação do STAI no grupo experimental antes e depois da intervenção. Os índices de BFS no grupo experimental foram significativamente maiores do que os do grupo de controle antes e depois de uma intervenção. Não houve diferença significativa nos indicadores do grupo de controle. Conclusão: A intervenção psicológica pós-traumática tem um efeito positivo sobre a recuperação da saúde mental dos atletas universitários. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução carreira atléticas físico psicológicos Objetivo competições Métodos 1 pesquisa randomização póstraumática. póstraumática pós traumática. traumática dados Resultados Conclusão II

RESUMO Objetivo: Explorar a influência das atividades de turismo esportivo de lazer sobre a saúde dos turistas idosos. Método: Através da investigação das atividades de turismo de lazer esportivo de alguns turistas idosos que participaram de agências de viagem, o estado de saúde e qualidade de vida dos turistas idosos que participaram de atividades de turismo de lazer esportivo por um longo tempo (grupo de exercícios) foram comparados com aqueles que não participaram ou ocasionalmente participaram de exercícios físicos (grupo não praticante), para entender a influência das atividades de turismo de lazer esportivo sobre a saúde dos turistas idosos. Resultados: Quanto à atitude de participação em atividades de turismo de lazer esportivo, os resultados da pesquisa mostram que os turistas idosos chegaram a um consenso básico sobre a participação em atividades de turismo de lazer esportivo. Diferentes tipos de paisagens têm diferenças significativas para melhorar a ansiedade dos turistas. A proporção de “muito satisfeitos” no grupo de exercícios foi significativamente maior do que a do grupo de não-exercícios. A duração da viagem tem um impacto significativo na melhoria da ansiedade dos turistas. Conclusão: Devemos fortalecer a propaganda das atividades de turismo esportivo de lazer, deixar que mais turistas idosos participem das atividades de turismo esportivo de lazer, fortalecer a orientação do comportamento dos turistas idosos em atividades de turismo esportivo de lazer, e ajudá-los a melhorar sua saúde. Nível de evidência II; Estudos terapêuticos – investigação de resultados de tratamento.

ABSTRACT Objective: To explore the influence of leisure sports tourism activities on the health of elderly tourists. Methods: Through investigating the leisure sports tourism activities of some elderly tourists who participated in travel agencies, the health status and quality of life of elderly tourists who participated in leisure sports tourism activities for a long time (exercising group) were compared with those who did not or occasionally participated in physical exercise (non-exercising group), to understand the influence of leisure sports tourism activities on the health of elderly tourists. Results: Regarding the attitude of participating in sports leisure tourism activities, the survey results show that the elderly tourists reached a basic consensus about participating in sports leisure tourism activities. Different types of landscapes have significant differences in improving tourists’ anxiety. The proportion of “very satisfied” in the exercise group was significantly higher than that in the non-exercise group. The duration of the trip has a significant impact on improving tourists’ anxiety. Conclusion: We should strengthen the advertising of leisure sport tourism activities, let more elderly tourists participate in leisure sport tourism activities, strengthen the behavioral guidance of elderly tourists in leisure sport tourism activities, and help them improve their health. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Objetivo: Explorar la influencia de las actividades de turismo deportivo de ocio en la salud de los turistas de edad avanzada. Método: Mediante la investigación de las actividades de turismo deportivo de ocio de algunos turistas de la tercera edad que participaron en agencias de viajes, se comparó el estado de salud y la calidad de vida de los turistas de la tercera edad que participaron en actividades de turismo deportivo de ocio durante un largo periodo (grupo ejercitante) con los que no hicieron ejercicio físico o lo hicieron ocasionalmente (grupo no ejercitante), para comprender la influencia de las actividades de turismo deportivo de ocio en la salud de los turistas de la tercera edad. Resultados: En cuanto a la actitud de participación en actividades de turismo de ocio deportivo, los resultados de la investigación muestran que los turistas de edad avanzada llegaron a un consenso básico sobre la participación en actividades de turismo de ocio deportivo. Los distintos tipos de paisajes presentan diferencias significativas a la hora de mejorar la ansiedad de los turistas. La proporción de “muy satisfechos” en el grupo de ejercicio fue significativamente mayor que en el grupo de no ejercicio. La duración del viaje tiene un impacto significativo en la mejora de la ansiedad de los turistas. Conclusión: Debemos reforzar la publicidad de las actividades de turismo deportivo de ocio, dejar que más turistas de edad avanzada participen en las actividades de turismo deportivo de ocio, reforzar la orientación del comportamiento de los turistas de edad avanzada en las actividades de turismo deportivo de ocio y ayudarles a mejorar su salud. Nivel de evidencia II; Estudios terapéuticos – investigación de resultados de tratamiento.

Abstract Objective: To investigate the effects of Erector Spinae Plane Block (ESPB) and Retrolaminar Block (RLB) on intraand postoperative analgesia in patients with Multiple Rib Fractures (MRFs). Methods: A total of 80 MRFs patients were randomly divided into the ESPB (Group E) and RLB (Group R) groups. After general anesthesia, ESPB and RLB were performed under ultrasound guidance, respectively, together with 20 mL of 0.5% ropivacaine and Patient-Controlled Intravenous Analgesia (PCIA). Results: Thirty-four cases in Group E and 33,cases in Group R showed unclear paravertebral spaces. The intraoperative dosage of remifentanil (mean ± SD) (392.8 ± 118.7 vs. 501.7 ± 190.0 μg) and postoperative morphine PCIA dosage, (7.35 ± 1.55 vs. 14.73 ± 2.18 mg) in Group R were significantly less than those in Group E; the Visual Analog Scale (VAS) scores in Group R at 2 (2.7 ± 1.2 vs. 3.4 ± 1.4), 4 (2.2 ± 1.1 vs. 2.8 ± 0.9), 12 (2.5 ± 0.9 vs. 3.0 ± 0.8), and 24 hours (2.6 ± 1.0 vs. 3.1 ± 0.9) after surgery were significantly lower than those in Group E. Finally, the normal respiratory diaphragm activity (2.17 ± 0.22 vs. 2.05 ± 0.19), pH (median [IQR] (7.38 [7.31-7.45] vs. 7.36 [7.30-7.42]), and partial pressure of carbon dioxide (PaCO2) (44 [35-49] vs. 42.5 [30-46]) after the operation in Group R were significantly better than those in Group E (p < 0.05). Conclusions: RLB was a more effective analgesic method than ESPB in the treatment of MRF.

Abstract Introduction Allergic rhinitis is a form of IgE mediated inflammation of the nasal mucosa in response to specific allergens, resulting in typical symptoms. Objectives This study was designed with the primary goal of comparing the clinical efficacy of posterior nasal neurectomy with or without pharyngeal neurectomy for the treatment of moderate-to-severe perennial allergic rhinitis. Secondary study aims included a comparison of the severity of comorbidities, including chronic cough and asthma, between patients in these two surgical treatment groups. Methods A total of 52 patients were enrolled in this randomized controlled trial and were assigned to either the control group (posterior nasal neurectomy) or the experimental group (posterior nasal neurectomy + pharyngeal neurectomy). The visual analog scale and rhinoconjunctivitis quality of life questionnaire were used to compare the differences in patient symptoms between baseline and 6-, 12-, and 24-months post-treatment. In addition, patient cough and asthma symptoms were monitored during follow-up via visual analog scale and asthma control test respectively. Results No significant differences in preoperative scores were evident between groups (p> 0.05). At 6-months post-treatment, there were significant differences in visual analog scale, rhinoconjunctivitis quality of life questionnaire, and asthma control test scores relative to baseline values in experimental group and control group patients (p< 0.05), and this remained true upon 12- and 24-month follow-up. No significant differences in visual analog scale, rhinoconjunctivitis quality of life questionnaire, or asthma control test scores were observed between the two treatment groups at any postoperative follow-up time point (p> 0.05), while coughing severity was found to be significantly reduced in the experimental group relative to the control group (p< 0.05). Conclusion posterior nasal neurectomy can be safely implemented with or without pharyngeal neurectomy in order to effectively treat allergic rhinitis. Combined posterior nasal neurectomy and pharyngeal neurectomy treatment may offer greater value than posterior nasal neurectomy alone for the treatment of allergic rhinitis patients with chronic cough. allergens moderatetosevere moderate severe comorbidities 5 neurectomy. . 6, 6 , 6- 12, 12 24months months 24 posttreatment. posttreatment post treatment. post-treatment addition followup follow up respectively p> p (p 0.05. 005 0.05 0 05 0.05) 6months posttreatment, treatment, p< 0.05, 24month month followup. up. 1 2 00 0.0 0.

Resumo Introdução A rinite alérgica é uma forma de inflamação da mucosa nasal mediada por IgE em resposta a alérgenos específicos, resulta em sintomas típicos. Objetivos Comparar a eficácia clínica da neurectomia nasal posterior com ou sem neurectomia faríngea para o tratamento da rinite alérgica perene de moderada a grave. Além disso, comparar a gravidade das comorbidades, inclusive tosse crônica e asma, entre os pacientes nesses dois grupos de tratamento cirúrgico. Método Foram incluidos neste ensaio clínico randomizado e designados para o grupo controle (neurectomia nasal posterior) ou para o grupo experimental (neurectomia nasal posterior + neurectomia faríngea) 52 pacientes. A escala visual analógica e o questionário de qualidade de vida na rinoconjuntivite (rhinoconjunctivitis quality of life questionnaire) foram usados para comparar as diferenças nos sintomas dos pacientes entre o período inicial e 6, 12 e 24 meses após o tratamento. Além disso, a tosse e os sintomas de asma dos pacientes foram monitorados durante o acompanhamento por meio da escala visual analógica e do teste de controle da asma (asthma control test ), respectivamente. Resultados Nenhuma diferença significante nos escores pré‐operatórios foi evidenciada entre os grupos (p > 0,05). Aos seis meses pós‐tratamento, houve diferenças significantes nos escores da escala visual analógica, no questionário de qualidade de vida na rinoconjuntivite e no teste de controle de asma em relação aos valores basais dos pacientes no grupo experimental e no grupo controle (p < 0,05), o que permaneceu verdadeiro após 12 e 24 meses de acompanhamento. Não foram observadas diferenças significantes nos escores da escala visual analógica e nem no questionário de qualidade de vida para conjuntivite ou no teste de controle da asma entre os dois grupos de tratamento em qualquer momento do acompanhamento pós‐operatório (p > 0,05), enquanto a gravidade da tosse foi significantemente reduzida no grupo experimental em relação ao grupo controle (p < 0,05). Conclusão A neurectomia nasal posterior pôde ser feita com segurança com ou sem neurectomia faríngea para o tratamento eficaz da rinite alérgica. O tratamento combinado com neurectomia nasal posterior e neurectomia faríngea pode oferecer mais benefício do que a neurectomia nasal posterior isolada para o tratamento de pacientes com rinite alérgica e tosse crônica. específicos típicos grave disso comorbidades cirúrgico 5 rhinoconjunctivitis questionnaire 6 1 2 asthma , ) respectivamente préoperatórios pré operatórios p 0,05. 005 0,05 . 0 05 0,05) póstratamento pós pós‐tratamento 0,05, pósoperatório operatório 00 0,0 0,

Resumo: Governança significa a política e a estratégia adotadas no processo de gestão de um país. Na longa história de mais de dois mil anos, o confucionismo tornou-se o núcleo cultural e a doutrina política da autocracia imperial, e a teoria da “governança de todo o país” contida nele tornou-se uma categoria importante na cultura tradicional chinesa. Deve-se aprofundar na rica conotação do pensamento de governança confucionista e construir um sistema de governança nacional que se adapte à sociedade, à situação geral e ao futuro, por meio de desenvolvimento inovador e transformação criativa, de modo a permitir que a excelente cultura tradicional chinesa retorne à base, para abrir um novo caminho e fazer o passado servir ao presente, fornecendo, assim, importante orientação teórica e referência histórica para o grande rejuvenescimento da nação chinesa.

Abstract: Governance means the policy and strategy adopted in the process of running a country. In the long history of more than two thousand years, Confucianism had become the cultural core and political doctrine of the imperial autocracy, and the theory of “governance of the whole country” contained in it had become an important category in traditional Chinese culture. We should dig deep into the rich connotation of Confucian governance thought, and build a national governance system that suits society, the overall situation and future through innovative development and creative transformation, so as to let excellent traditional Chinese culture return to open a new road and make the past serve the present, thus providing important theoretical guidance and historical reference for the great rejuvenation of the Chinese nation.

RESUMO Introdução A atividade física regular ajuda a melhorar as habilidades cardiovasculares e cerebrovasculares. Como avaliar a tensão nervosa dos vasos cardiovasculares e cerebrovasculares por meio de esportes é um assunto quente. Objetivo o artigo discute a influência da participação regular em esportes sobre a função cardiovascular das pessoas e indicadores relacionados ao sangue. Métodos Selecionamos 30 idosos saudáveis que participam regularmente de esportes, registramos suas alterações no ECG, pressão arterial, frequência cardíaca e outros indicadores relacionados à função cardiovascular e analisamos a função sanguínea dos idosos. Detecção de contagem de células sanguíneas (RBC), volume de glóbulos vermelhos (MCV) e hemoglobina (Hb), creatinina sérica (Cr), glicose no sangue (BGS), triglicerídeos (TG), colesterol (TC), lipoproteína de baixa densidade (LDL ) e a lipoproteína de alta densidade (HDL) é medida. Resultados Idosos que persistem por muito tempo nos exercícios têm melhores indicadores do que aqueles que não praticam. Conclusão O exercício aeróbio adequado pode reduzir a rigidez dos vasos sanguíneos em idosos. O exercício pode ajudar os idosos a aumentar a variabilidade da frequência cardíaca e melhorar os indicadores sanguíneos da função nervosa autonômica do coração e a massa corporal. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction Regular physical activity helps improve cardiovascular and cerebrovascular skills. How to evaluate the nervous tension of the cardiovascular and cerebrovascular vessels through sports is a hot topic. Objective The paper discusses the influence of regular participation in sports on people’s cardiovascular function and blood-related indicators. Methods We select 30 healthy older adults who regularly participate in sports, record their ECG changes, blood pressure, heart rate and other related cardiovascular function indicators, and analyze the blood function of the elderly. Detection of blood cell count (RBC), red blood cell volume (MCV) and hemoglobin (Hb), serum creatinine (Cr), blood glucose (BGS), triglycerides (TG), cholesterol (TC), low-density lipoprotein (LDL) and high-Density lipoprotein (HDL) is measured. Results Older adults who persist in exercise for a long time have better indicators than those who do not exercise. Conclusions Appropriate aerobic exercise can reduce the stiffness of blood vessels in the elderly. Exercise can help the elderly increase heart rate variability and improve the heart’s autonomic nerve function’s blood indicators, and body mass. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Introducción La actividad física regular ayuda a mejorar las habilidades cardiovasculares y cerebrovasculares. Cómo evaluar la tensión nerviosa de los vasos cardiovasculares y cerebrovasculares a través del deporte es un tema candente. Objetivo El artículo analiza la influencia de la participación regular en deportes sobre la función cardiovascular de las personas y los indicadores relacionados con la sangre. Métodos Seleccionamos a 30 adultos mayores sanos que participan regularmente en deportes, registramos sus cambios en el ECG, presión arterial, frecuencia cardíaca y otros indicadores relacionados con la función cardiovascular, y analizamos la función sanguínea de los ancianos. Detección del recuento de glóbulos rojos (RBC), volumen de glóbulos rojos (MCV) y hemoglobina (Hb), creatinina sérica (Cr), glucosa en sangre (BGS), triglicéridos (TG), colesterol (TC), lipoproteínas de baja densidad (LDL) ) y se mide la lipoproteína de alta densidad (HDL). Resultados Los adultos mayores que persisten en el ejercicio durante mucho tiempo tienen mejores indicadores que los que no lo hacen. Conclusión El ejercicio aeróbico adecuado puede reducir la rigidez de los vasos sanguíneos en los ancianos. El ejercicio puede ayudar a los ancianos a aumentar la variabilidad de la frecuencia cardíaca y mejorar los indicadores sanguíneos y la masa corporal de la función nerviosa autónoma del corazón. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.

Abstract It has been extensively reported that long noncoding RNAs (lncRNAs) were closely associated with multiple malignancies. The aim of our study was to investigate the effects and mechanism of lncRNA POU6F2-AS1 in lung adenocarcinoma (LADC).The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets provided us the information of LADC clinical samples. High-regulation of POU6F2-AS1 was presented in LADC tissues compared with adjacent normal tissues, which was correlated with poor outcome of LADC patients. Functional experiments in Calu-3 and NCI-H460 cells showed that POU6F2-AS1 significantly promoted LADC cell proliferation, colony formation, invasion and migration. Moreover, through online prediction, luciferase reporter assay and Pearson’s correlation analysis, we found that POU6F2-AS1 may act as a competing endogenous RNA (ceRNA) of miR-34c-5p and facilitated the expression of potassium voltage-gated channel subfamily J member 4 (KCNJ4). The promoting effect of cell aggressiveness induced by POU6F2-AS1 was enhanced by KCNJ4, whilst was abrogated due to the overexpression of miR-34c-5p. Collectively, POU6F2-AS1 might function as a ceRNA through sponging miR-34c-5p to high-regulate KCNJ4 in LADC, which indicates that POU6F2-AS1 might be a promising therapeutic target with significant prognostic value for LADC treatment.

RESUMO Introdução: A acupuntura tem sido amplamente utilizada para alívio de dor. No entanto, seus mecanismos são muito pouco conhecidos. Objetivo: Investigar a relação entre o efeito analgésico da eletroacupuntura e a regulação da expressão de adenosina e de substância P. Métodos: Utilizou-se um modelo de dor inflamatória crônica em ratos por injeção única do Adjuvante Completo de Freund e, em seguida, os animais foram tratados com eletroacupuntura diariamente. Foram aplicadas sete sessões bilaterais de eletroacupuntura (ST36 e BL60, 0,5 a 1,5 mA, força inicial de 0,5 mA, aumentada em 0,5 mA a cada 10 minutos, 30 minutos por sessão, uma sessão por dia) em ratos com Adjuvante Completo de Freund, por sete dias. O efeito analgésico da eletroacupuntura foi avaliado pela medida do limiar de retirada da pata em ratos que receberam estimulações mecânica e térmica. Resultados: A estimulação diária com eletroacupuntura aumentou efetivamente o limiar de retirada da pata em ratos com Adjuvante Completo de Freund. A eletroacupuntura aumentou o nível de adenosina na região zusanli. Estudos posteriores mostraram que a eletroacupuntura poderia diminuir os níveis de substância P, receptor de neurocinina-1, fator de necrose tumoral-alpha, interleucina-1β, interleucina-6 e CD68 nos gânglios da raiz dorsal. Curiosamente, a injeção direta de antagonistas do receptor de adenosina A1 ou de substância P, ou a transecção da raiz do nervo dorsal, podem prejudicar significativamente as ações analgésicas induzidas pela eletroacupuntura em ratos com Adjuvante Completo de Freund e reduzir os níveis de substância P, receptor de neurocinina-1, fator de necrose tumoral-alfa, interleucina-1β, interleucina-6 e CD68. Por fim, confirmamos que a injeção direta de um agonista do receptor da adenosina A1 reproduziu os efeitos analgésicos da eletroacupuntura. Conclusão: Nossos resultados indicam a regulação da secreção da substância P mediada pela adenosina. A via mediada pela substância P pode estar envolvida no processo de analgesia por eletroacupuntura em ratos.

ABSTRACT Background: Acupuncture has been widely used for alleviating pain. However, its mechanisms remain largely enigmatic. Objective: In the present study, we focused on whether the analgesic effect of electroacupuncture is related to its regulation on adenosine and substance P expression. Methods: We established chronic inflammatory pain model in rats through a single injection of Complete Freund’s Adjuvant, and then we treated animals using daily electroacupuncture. We applied seven bilateral sessions of electroacupuncture (ST36 and BL60, 0.5 to 1.5 mA, initial strength of 0.5 mA, increased by 0.5 mA every 10 minutes, for 30 minutes per session, one section per day) to Complete Freund’s Adjuvant rats for seven days. The analgesic effect of electroacupuncture was evaluated by measuring paw withdrawal threshold in rats that received mechanical and thermal stimulation. Results: Daily electroacupuncture stimulation effectively increased paw withdrawal threshold in Complete Freund’s Adjuvant rats. Electroacupuncture increased the adenosine level in zusanli. A further study showed that electroacupuncture could decrease substance P, neurokinin-1 receptor, tumor necrosis factor-alpha, interleukin-1β, interleukin-6 and CD68 levels in dorsal root ganglion. Interestingly, direct injection of adenosine A1 or substance P receptor antagonists, or dorsal nerve root transection could significantly impair electroacupuncture induced analgesic actions in Complete Freund’s Adjuvant rats could and reduce the levels of substance P, neurokinin-1 receptor, tumor necrosis factor-alpha, interleukin-1β, interleukin-6 and CD68. Finally, we confirmed that direct injection of adenosine A1 receptor agonist replicated the analgesic effect of electroacupuncture. Conclusion: Our results indicate regulation of adenosine-mediated substance P secretion. Substance P-mediated pathway may be involved in the analgesia process by electroacupuncture in rats.