ABSTRACT Aimed at the deficiency of existing PID controller for quad rotor UAV, a single neuron PID controller with gravity compensation is presented. After using feed forward control to compensate gravity, the position loop adopts PID control to ensure control accuracy, while the attitude loop adopts single neuron control to increase adaptive ability. Then, by using Matlab/simulink simulation software, the position control of quad rotor UAV is carried out, and the simulation result shows, compared with the traditional double closed loop PID controller, the control algorithm based on the Single Neuron adaptive PID with gravity compensation can effectively improve the robustness and adaptability of the quad rotor UAV system. presented accuracy ability Then Matlabsimulink Matlab simulink software out shows system

Abstract Background and objective Systemic sclerosis (SSc) is an immune-mediated rheumatic disease characterized by fibrosis and vascular lesions. Interstitial lung disease is an early complication of SSc and the main cause of death from SSc. Although baricitinib shows good efficacy in a variety of connective tissue diseases, its role in systemic sclerosis-related interstitial lung disease (SSc-ILD) is unclear. The objective of our study was to explore the effect and mechanism of baricitinib in SSc-ILD. Methods We explored crosstalk between the JAK2 and TGF-β1 pathways. In vivo experiments, SSc-ILD mice model were constructed by subcutaneous injection of PBS or bleomycin (7.5 mg/kg) and intragastric administration of 0.5% CMC-Na or baricitinib (5 mg/kg) once every two days. We used ELISA, qRT-PCR, western blot and immunofluorescence staining to evaluate the degree of fibrosis. In vitro experiments, we used TGF-β1 and baricitinib to stimulate human fetal lung fibroblasts (HFLs) and assessed protein expression by western blot. Results The vivo experiments showed that baricitinib notably alleviated skin and lung fibrosis, decreased the concentration of pro-inflammatory factors and increased those of the anti-inflammatory factors. Baricitinib affected the expression of TGF-β1 and TβRI/II inhibitiing JAK2. In the vitro experiments, following the culture of HFLs with baricitinib or a STAT3 inhibitor for 48 h, the expression levels of TβRI/II decreased. Conversely, with successful inhibition of TGF-β receptors in HFLs, JAK2 protein expression decreased. Conclusions Baricitinib attenuated bleomycin-induced skin and lung fibrosis in SSc-ILD mice model by targeting JAK2 and regulating of the crosstalk between the JAK2 and TGF-β1 signaling pathways. (SSc immunemediated immune mediated lesions diseases sclerosisrelated related SScILD ILD (SSc-ILD unclear SScILD. ILD. JAK TGFβ1 TGFβ TGF β1 β pathways 7.5 75 7 5 (7. mg/kg mgkg mg kg 05 0 0.5 CMCNa CMC Na ( days ELISA qRTPCR, qRTPCR qRT PCR, PCR qRT-PCR (HFLs proinflammatory pro inflammatory antiinflammatory anti TβRIII TβRI II STAT 4 h Conversely bleomycininduced induced 7. (7 0.

Abstract In the current study the incidence of food impaction between implant-supported fixed dental prostheses and adjacent teeth was determined and the factors that influence food impaction were identified. Seven hundred seventy-nine implant prosthetic crowns and 879 adjacent sites from a pool of 489 patients were retrospectively studied. Food impaction existed in 16.6% of patients with implant restorations at the follow-up evaluations. Food impaction occurred more frequently on the mesial aspect of the implant prosthesis and the molar area. Among the factors that affected food impaction, proximal contact loss was shown to be the major cause (78.6%), while the incidence of food impaction on the mesial and distal aspects was 58.0% and 20.6%, respectively. Based on single-factor analysis of variance, gender, implant location, mesial and distal aspects of the prosthesis, and lost time before implant restoration were the factors that influenced proximal contact loss in patients (P < 0.05). In conclusion, proximal contact loss was shown to be highly associated with food impaction. Thus, gender, the molar area, the mesial aspect of the prosthesis, and the lost time before implant restoration should be fully considered. Guidance on the proper use and maintenance of prostheses is essential to achieve an optimal outcome.

Abstract Background Stathmin as a critical protein involved in microtubule polymerization, is necessary for survival of cancer cells. However, extremely little is known about Stathmin in glioblastoma. So, this study was designed to elucidate the function of Stathmin gene in the tumorigenesis and progression of glioblastoma cells. Method The lentiviral interference vector pLV3-si-Stathmin targeting Stathmin gene and the control vector pLV3-NC were established for the co-transfection of 293T cells together with the helper plasmids. Viral titer was determined via limiting dilution assay. Then pLV3-si-Stathmin and pLV3-NC were stably co-transfected into U373 and U87-MG glioblastoma cells. Expression levels of Stathmin protein in each group were determined by using Western Blot, and the proliferation and migration ability of the cells with downregulated Stathmin were evaluated through CCK8 assay and transwell invasion assay, respectively. Cell cycles and cell apoptosis were detected with flow cytometry. Finally, the effect of Stathmin in tumor formation was determined in nude mice. Result DNA sequencing and viral titer assay indicated that the lentiviral interference vector was successfully established with a viral titer of 4 × 108 TU/ml. According to the results from Western Blotting, Stathmin protein expression level decreased significantly in the U373 and U87-MG cells after transfected with pLV3-si-Stathmin, respectively, compared with those transfected with pLV3-NC. In glioblastoma cells, the cell proliferation and migration were greatly inhibited after the downregulation of Stathmin protein. Flow cytometry showed that much more cells were arrested in G2/M phasein Stathmin downregulated group, compared with the non-transfection group and NC group. But Stathmin downregulation did not induce significant cell apoptosis. Tumor formation assay in nude mice showed that tumor formation was delayed after Stathmin downregulation, with a reduction in both tumor formation rate and tumor growth velocity. Conclusion Stathmin downregulation affected the biological behaviors of U373 and U87-MG glioblastoma cells, inhibiting the proliferation and migration of tumor cells. Stathmin gene may serve as a potential target in gene therapy for glioblastoma.

Uma das tendências contemporâneas globais é o desenvolvimento da chamada medicina "transacional". A estrutura tradicional de hospitais não atende às demandas implicadas no desenvolvimento desta nova forma de investigação; explorar novas estruturas hospitalares torna-se imperativo. Atento às demandas atuais, o governo chinês propôs e implementou uma estratégia para a implantação de hospitais de primeira-classe, orientados para a pesquisa moderna em Medicina. A implantação de unidades assistenciais com características de hospitais orientados para a pesquisa médica de fato mostrou-se uma estratégia importante para orientar o desenvolvimento científico de instituições de alta qualidade e para fomentar o desenvolvimento da investigação transacional. Para catalisar estas ações, criou-se a Associação Chinesa de Hospitais de Pesquisa, que provê assistência médica, estimula o desenvolvimento de jovens talentos, desenvolve pesquisa médico-científica e atua na formação médica, cobrindo áreas de pesquisa teórica e prática, intercâmbios acadêmicos e formação de recursos humanos. De maneira geral, hospitais acadêmicos, orientados à pesquisa facilitam a tradução dos conhecimentos entre as diversas áreas da Medicina, estimulando a criação de plataformas interdisciplinares, treinando competências essenciais em pesquisa clínica e translacional, provendo suporte para a geração de conhecimento e sua difusão por meios acadêmicos.

Globally, one of the major trends is the development of translational medicine. The traditional hospital structure could not meet the demands of translational medicine development any longer and to explore a novel hospital structure is imperative. Following the times, China proposed and implemented a development strategy for a first-class modern research-oriented hospital. To establish a research-oriented hospital has become an important strategy to guide the scientific development of high-quality medical institutions and to advance translational medicine development. To facilitate translational medicine by developing research-oriented hospital, the Chinese Research Hospital Association (CRHA) has been established, which provides service of medicine, talents cultivation, scientific research and clinical teaching and covers areas of theoretical research, academic exchange, translational medicine, talents training and practice guiding. On the whole, research-oriented hospital facilitated translational medicine by developing interdisciplinary platform, training core competencies in clinical and translational research, providing financial support of translational research, and hosting journals on translational medicine, etc.