Abstract IE(%) of CORP (with AN of 7.8 and 3.5., and modified with Zn powder, MGr or MCNT) used as coatings against CS atmospheric corrosion has been investigated. The studies were carried out by gravimetry and PSP methods. CORP composition was: phosphatides, hydrated oil and SF. IE(%) effect of CO coatings with the investigated compositions on CS corrosion kinetic parameters was estimated in a 0.5 M NaCl solution. Zn powder (50 wt.%) addition to CORP increased their IE(%) up to 80-86%. In this case, the coatings IE(%), at an anodic E of -0.2 V, reached 93-99%. The addition of MGr or MCNT to CORP modified by Zn powder had mostly a negative effect on IE(%).

This article puts forward love, care, and reverence for all people and our nonhuman kin as the center of posthumanist education. Opening our spiritual eye and building intimate relationships with nature and with all elements of the universe is envisioned as part of a schooling or post-schooling experience; our body, heart and spirit, along with our mind, is an essential part of learning. The article describes the experiences of students taking a graduate level course focusing on global climate change and education at a university in the United States. We use the course as an example to explore what posthumanist education entails. In this qualitative study, students taking the course, along with the faculty designing and teaching the course, describe the course curriculum and pedagogies and reflect on the course's impact on them. Data sources include the syllabus, students' reflection papers, nature contact journals, final projects, art works, and group conversations. Although situated in North America, the article is rich with international perspectives as student authors came from six different countries. The article posits that posthumanist education must be eco-centric and love-based, engaging students' whole being to feel for and love Mother Nature.

Assessments plays an important role in Higher Education as a cornerstone and driver of learning and teaching activities, curriculum development and renewal, achievement of learning objectives and student academic success, as well as the development of graduate attributes. Assessment load, however, in terms of over-assessment (along with under-assessment), is a blurred concept that is difficult to define qualitatively and measure quantitatively. To address this gap in research, based on a comprehensive literature review, we developed a multidimensional assessment index (MAI), with eight indicators, to measure over-assessment. These indicators include features of continuous assessment (total number, mark allocation or size, weighting, degree of difficulty, frequency and scheduling, type, content coverage and repetition) and the presence, or absence, of a single high-stakes final examination. We applied these indicators to a case study of an undergraduate Economics, consisting of 12 modules across 3 years of study, at a university in South Africa. The MAI was then compared with the pass rate, as a measure of student success, and there was a moderate and negative correlation between the MAI (high assessment loads) and pass rate variables. Lastly, the indicator with the strongest correlation with pass rate was the length of assessments (or proportion of continuous assessments with a high mark count). It is hoped that this study helps fill the gaps in existing literature on definition and measurement of assessment loads, and the practical use of the MAI as a tool to evaluate assessment load and inform the design of assessment for student learning and success.

Abstract Objectives To evaluate the effects of matrix metalloproteinase (MMP) and cathepsin K (catK) inhibitors on resistance to dentin erosion. Methodology A total of 96 dentin specimens (3×3×2 mm) were prepared and randomly assigned into four groups (n=24): deionized water (DW); 1 µM odanacatib (ODN, catK inhibitor); 1 mM 1,10-phenanthroline (PHEN, MMP inhibitor); and 1 µM odanacatib + 1 mM 1,10-phenanthroline (COM). Each group was further divided into two subgroups for the application of treatment solutions before (PRE) and after erosive challenges (POST). All specimens were subjected to four daily erosive challenges for 5 d. For each erosive challenge, the specimens in subgroup PRE were immersed in the respective solutions before cola drinks, while the specimens in subgroup POST were immersed in the respective solutions after cola drinks (the immersion duration was 5 min in both cases). All specimens were stored in artificial saliva at 37°C between erosive challenges. The erosive dentin loss (EDL) was measured by profilometry. The residual demineralized organic matrix (DOM) of specimens was removed using type VII collagenase and evaluated by profilometry. Both the EDL and thickness of the residual DOM were statistically analyzed by two-way analysis of variance (ANOVA) and Bonferroni’s test (α=0.05). The surface topography and transverse sections of the specimens were observed using SEM. MMPs and catK were immunolabeled in the eroded dentin and in situ zymography was performed to evaluate the enzyme activity. Results Significantly lower EDL was found in the groups ODN, PHEN, and COM than in the control group (all p<0.05), while no significant difference in EDL was found among the groups ODN, PHEN, and COM (all p>0.05). The application sequence showed no significant effect on the EDL of the tested groups (p=0.310). A significantly thicker DOM was observed in the group ODN than in the control group regardless of the application sequence (both p<0.05). The treatment with ODN, PHEN, and COM inhibited the gelatinolytic activity by approximately 46.32%, 58.6%, and 74.56%, respectively. Conclusions The inhibition of endogenous dentinal MMPs and catK increases the acid resistance of human dentin but without an apparent synergistic effect. The inhibition of MMPs and catK is equally effective either before or after the acid challenge. (MMP (catK erosion 9 3×3×2 332 3 2 (3×3× mm n=24 n24 n 24 (n=24) DW (DW) (ODN inhibitor inhibitor) 1,10phenanthroline 110phenanthroline phenanthroline 1,10 10 PHEN (PHEN COM. . (COM) (PRE POST. (POST) d challenge cases. cases cases) 37C C 37 (EDL profilometry (DOM twoway way ANOVA (ANOVA Bonferronis Bonferroni s α=0.05. α005 α α=0.05 0 05 (α=0.05) SEM all p<0.05, p005 p p<0.05 , p<0.05) p>0.05. p>0.05 p>0.05) p=0.310. p0310 p=0.310 310 (p=0.310) p<0.05. 4632 46 32 46.32% 586 58 6 58.6% 7456 74 56 74.56% respectively 3×3× 33 (3×3 n=2 n2 (n=24 (DW 10phenanthroline 110 1,1 (COM (POST α00 α=0.0 (α=0.05 p00 p<0.0 p>0.0 p031 p=0.31 31 (p=0.310 463 4 46.32 58.6 745 7 74.56 3×3 (3× n= (n=2 11 1, α0 α=0. (α=0.0 p0 p<0. p>0. p03 p=0.3 (p=0.31 46.3 58. 74.5 3× (3 (n= α=0 (α=0. p<0 p>0 p=0. (p=0.3 46. 74. ( (n α= (α=0 p< p> p=0 (p=0. (α= p= (p=0 (α (p= (p

Abstract Introduction Prior studies have found inconsistent results regarding the relationship between vitamin D status and Idiopathic Central Precocious Puberty (ICPP). Objective To assess the role of serum 25-hydroxyvitamin D (25 [OH]D) levels in ICPP development. Method The authors retrospectively collected data from 221 girls with ICPP and 144 healthy girls between January 2017 and December 2019. The participants’ serum 25(OH)D levels were measured using an automatic chemiluminescence method, and the association between serum 25(OH)D levels and the risk of ICPP was assessed using multivariate logistic regression analysis. Odds Ratios (OR) with 95% Confidence Intervals (95% CI) were calculated as effect estimates. Results Serum 25(OH)D levels in the ICPP group were significantly lower than those in healthy controls (p < 0.001). Multivariate analysis indicated that girls with insufficient vitamin D levels (OR = 0.201; 95% CI 0.094-0.428; p < 0.001) and sufficient vitamin D levels (OR = 0.141; 95% CI 0.053-0.375; p < 0.001) both had a lower risk of ICPP than girls with vitamin D deficiency. Moreover, the authors found that the height (p = 0.014), weight (p = 0.014), breast stage (p = 0.010), mother's height (p < 0.001), and luteinizing hormone/follicle-stimulating hormone ratio (p = 0.010) in girls with ICPP could be associated with levels of vitamin D. Conclusion This study found that a low serum 25(OH)D level is an independent risk factor for ICPP, and several characteristics of girls with ICPP could be affected by their vitamin D status. ICPP. . (ICPP) 25hydroxyvitamin hydroxyvitamin 25 (2 OHD OH [OH]D development 22 14 201 2019 participants 25OHD method OR 95 (95 estimates 0.001. 0001 0.001 0 001 0.201 0201 0.0940.428 00940428 0.094 0.428 094 428 0.094-0.428 0.141 0141 141 0.0530.375 00530375 0.053 0.375 053 375 0.053-0.375 deficiency Moreover 0.014, 0014 0.014 , 014 0.014) 0.010, 0010 0.010 010 mothers mother s 0.001, hormone/folliclestimulating hormonefolliclestimulating hormone/follicle stimulating follicle (ICPP 2 ( 1 20 9 (9 000 0.00 00 0.20 020 0940 0.0940.42 0094042 0094 0.09 0428 0.42 09 42 0.094-0.42 0.14 0530 0.0530.37 0053037 0053 0.05 0375 0.37 05 37 0.053-0.37 0.01 01 folliclestimulating hormonefollicle 0.0 0.2 02 0.0940.4 009404 009 042 0.4 4 0.094-0.4 0.1 0.0530.3 005303 005 037 0.3 3 0.053-0.3 0. 0.0940. 00940 04 0.094-0. 0.0530. 00530 03 0.053-0. 0.0940 0.094-0 0.0530 0.053-0 0.094- 0.053-

Abstract The main idea of aviation in the 21st century is the safety of passengers and crew, but the human is always inclined towards comfort. The purpose of the article is to study the comfort requirements for the interior layout of long-haul aircraft. The concept of the fuselage layout with capsule accommodation of passengers was formed. It was stated that with a flight range of more than 8 hours, the flight is comfortable only in business or first classes. The proposed variant of capsule placement, designed for the Boeing 777-200LR cabin, can be installed in other passenger airliners with a ceiling height of at least 2.1 m. Also, it was indicated that the proposed two-story capsule placement allows to provide the layout with first-class seats in a given cabin. It was concluded that the installation of individual passenger cells in railway transport on the high-speed trains Strizh or Sobsan, in airport terminals as hotel accommodation also holds promises.

The correlation between the level of difficulty of assessments, Bloom's Taxonomy as well as pass rates of courses has been a seriously under-researched area in South Africa. In this study, we proposed the revised Bloom's taxonomy level of difficulty index, before we examined 112 first-year 2017-2019 final and supplementary assessment papers from the Economic and Management Sciences Faculty of a university in Western Cape. The descriptive statistics showed that these assessment papers are different in terms of duration, total marks, type of questions asked as well as pass rates. It was also found that these first-year summative assessments asked questions mainly at levels two (understand) and three (apply) of the revised Bloom's Taxonomy. In addition, the correlation and econometric analysis did not find a strong correlation between the level of difficulty index and pass rates. Nonetheless, the above-mentioned results need to be interpreted with great caution, because strictly speaking, one should also control for differences in other characteristics (e.g., students' personal characteristics, school characteristics and lecture attendance). To conclude, there is no explicit national policy that guides higher education institutions (HEIs) on how to use Bloom's or any other taxonomy to assess students at the appropriate National Qualifications Framework (NQF) level. Hence, our findings suggest that there is a need for a national assessment policy framework to guide HEIs on how to assess undergraduate students at different cognitive levels as required by the NQF.

Studies conducted nationally found that students with Matric Accounting knowledge performed significantly better than students without it in university-level Accounting modules (Baard, Steenkamp, and Kidd 2010; Papageorgiou 2017; Steenkamp, Baard, and Frick 2009). However, the reality at South African universities is that Accounting as a school subject is not always a requirement to pursue BCom Accounting studies. This situation means that at certain universities, Accounting as a school subject is not taken into consideration for this degree, while this is the case at some universities. This study focuses on two cohorts of students enrolled for the mainstream programme in 2017-2019 at a South African university. The reason for focusing on these two cohorts was to establish the throughput rate of students with or without Matric Accounting knowledge. Two most significant findings of this study are that firstly, students who obtained at least 70 per cent for Matric Accounting completed the degree within the minimum three-year time frame, while students who obtained at least 80 per cent had a higher throughput rate of 48.8 per cent. Secondly, students who achieved lower than 70 per cent for Mathematics and did not complete Matric Accounting were unable to complete the degree in the minimum timeframe. The research methodology includes both quantitative and qualitative methods. The results can inform the selection and admission criteria at tertiary institutions and inform other stakeholders in higher education on how school subjects and grades influence students' throughput rate.

This study investigated the effects of Bacillus licheniformis-fermented products (BLFPs) on the growth performance, faecal microbiota, and antibiotic resistance gene (ARG) expression in weaning piglets on a commercial farm. Ninety-six weaning piglets were randomly assigned to four treatments as follows: basal diet as control (C), basal diet plus 30 mg/kg of antibiotics (bacitracin methylene disalicylate) (A), basal diet plus 1 g/kg of BLFPs (F), and basal diet plus 15 mg/kg of antibiotics and 0.5 g/kg of BLFPs (AF), with six replicate pens per treatment and four pigs per pen. Results showed that, similar to antibiotics, replacing all or half the antibiotics with BLFPs improved the feed conversion ratio of weaning piglets from 15-28 d. Microbiota analysis showed that microbial community composition in the faeces showed a clear separation between groups. Replacing all the antibiotics with BLFPs increased the abundance of the genus, Streptococcus, in the faeces compared with the other groups. Half replacement of antibiotics with BLFPs increased the chloramphenicol resistance gene levels in the faeces compared with the C group, whereas full replacement of antibiotics with BLFPs reduced the streptomycin resistance gene levels compared with the C group. A trend of decreased levels of formic acid and acetic acid was observed in the group treated with BLFPs in combination with antibiotics compared with the C group. In conclusion, the field study demonstrates that replacing all or half the antibiotics with BLFPs can improve feed conversion ratio, modulate faecal microbiota, and alter ARG expression in weaning piglets.

The academic success of first-year Economics students has been examined in many South African studies in Economic Education. These studies controlled for differences in demographic characteristics, last school examination (Matric) subjects and results, as well as lecture and tutorial attendance when investigating differences in students' performance. While there is an abundance of international studies investigating the main reasons for attendance or non-attendance, these studies are rare in the South African context, especially in the field of Economics. Hence, this study fills the existing local research gap by investigating factors influencing lecture attendance as well as their possible impact on the performance of first-year Microeconomics students at the University of the Western Cape. The key empirical findings suggest that both lecture and tutorial attendance had a positive and significant impact on both the likelihood of qualifying to write the examination as well as the examination mark. In addition, students who enrolled in Economics in Matric and obtained better marks in first-year Macroeconomics in the previous semester performed significantly better in the Microeconomics examination. It was also found that the main reasons for not attending lectures are academically related, with the top reason being "busy studying for tests". Furthermore, students who regarded tutorials as a replacement for lectures significantly suffered nearly five marks lower in the examination. We recommend revisions to teaching methods and making lecture attendance compulsory and part of assessments. Furthermore, given lecture attendance is low, revisions to timetables should be considered and expanded transportation be made available to students. Lastly, students should be given the necessary time management tools to adjust to greater workloads at university.

SUMMARY: The studies of the properties of vascular structures and tissues during electric welding, in particular direct morphological changes in the blood vessel walls in the areas of welding processes, are of interest. Perforating veins, femoral veins, abdominal aorta, vena cava and porcine perforating veins of the limbs were used in this study. We performed end-to-end electric welding of the aorta, venous end-to-side electric welding, vein end-to-artery side arterial and venous welding, venous end-to-end electric welding, as well as arterial and venous lumen sealing.The results of histological studies showed the formation of a coagulated acellular protein matrix, represented by unorganized denatured protein fibrous structures. In the area of vascular tissue coagulation, lacunes were formed as a result of water evaporation from the biological tissue. In the perifocal area of the welded junction, cell reduction occurred without necrosis or charring. The data obtained confirm the safety of high frequency electric welding of the main vessels and the prospectfor clinical use of the studied techniques.

RESUMEN: Los estudios de las propiedades de las estructuras y tejidos vasculares durante la soldadura eléctrica son relevantes, en particular los cambios morfológicos directos en las paredes de los vasos sanguíneos en las áreas de los procesos de soldadura. En este estudio se utilizaron venas perforantes, venas femorales, parte abdominal de la aorta, vena cava y venas perforantes porcinas de los miembros. Realizamos soldadura eléctrica de extremo a extremo de la parte abdominal de la aorta, soldadura eléctrica de extremo a lado venosa, soldadura arterial y venosa de extremo a arteria del lado venoso, soldadura eléctrica de extremo a extremo venoso, así como lumen arterial y venoso. Los resultados de los estudios histológicos mostraron la formación de una matriz de proteína acelular coagulada, representada por estructuras fibrosas de proteínas desnaturalizadas no organizadas. Se formaron lagunas como resultado de la evaporación del agua del tejido biológico en el área de la coagulación del tejido vascular, En el área perifocal de la unión soldada, la reducción celular ocurrió sin necrosis ni carbonización. Los datos obtenidos confirman la seguridad de la soldadura eléctrica de alta frecuencia de los vasos principales y la perspectiva de uso clínico de estas técnicas.

RESUMO O objetivo do presente estudo foi investigar os diferentes metabolitos do plasma entre o cio e o cio pós-parto de vacas leiteiras e fornecer uma base teórica para a prevenção do cio de vacas em fazendas de leite. No experimento, foram selecionadas 127 vacas leiteiras Holstein com idade e paridade similares. De acordo com a concentração de β- ácido hidroxibutírico, ácidos graxos não esterificados e glicose no plasma entre 14 e 21 dias no leite, todas as vacas leiteiras foram determinadas em estado de equilíbrio energético. De acordo com os resultados dos sintomas clínicos, do exame de ultra-som retal e B aos 60 a 90 dias pós-parto, estas vacas foram divididas em vinte cios e vinte e quatro grupos de cio, outras vacas leiteiras foram removidas. A tecnologia de ressonância magnética nuclear 1H foi utilizada para detectar as alterações dos metabólitos plasmáticos e para triar diferentes metabólitos plasmáticos entre as vacas do cio e do cio. Dez diferentes metabólitos incluindo alanina, ácido glutâmico, asparagina, creatina, colina, fosfocholina, glicerofosfocolina, lipoproteína de baixa densidade e lipoproteína de muito baixa densidade foram significativamente diminuídos nas vacas antróficas em comparação com as vacas estro. As análises da via metabólica indicaram que os metabólitos diferenciais estavam principalmente envolvidos no metabolismo de aminoácidos e glicerofosfolipídios. Estes metabólitos e suas vias de enriquecimento indicam que a redução dos precursores da síntese de hormônios esteróides resulta em níveis mais baixos de estradiol e progesterona e causa anestros no balanço energético negativo. Estes dados fornecem uma melhor compreensão das mudanças que podem afetar o cio das vacas leiteiras pós-parto no estado de NEB e preparam o terreno para mais pesquisas.

ABSTRACT The objective of the present study was to investigate the different plasma metabolites between anestrus and estrus postpartum dairy cows and to provide a theoretical basis for prevention of anestrus in dairy farm cows. In the experiment, one hundred and sixty-seven Holstein dairy cows were selected with similar age and parity. According to the concentration of β-hydroxybutyric acid, non-esterified fatty acids and glucose in plasma during 14 to 21 days in milk, all dairy cows were determined as having a status of energy balance. According to the results of clinical symptom, rectal and B ultrasound examination at 60 to 90 days postpartum, these cows were divided into twenty estrus and twenty-four anestrus group, other dairy cows were removed. 1H nuclear magnetic resonance technology was utilized to detect the plasma metabolites changes and screen different plasma metabolites between anestrus and estrus cows. Ten different metabolites including alanine, glutamic acid, asparagine, creatine, choline, phosphocholine, glycerophosphocholine, low-density lipoprotein, and very-low-density lipoprotein were significantly decreased in anestrous cows compared with estrous cows. Metabolic pathway analyses indicated that differential metabolites were primarily involved in amino acid and glycerophospholipid metabolism. These metabolites and their enrichment pathways indicate that reduced steroid hormone synthesis precursors result in lower levels of estradiol and progesterone and cause anestrus in negative energy balance. These data provide a better understanding of the changes that may affect estrus of postpartum dairy cows at NEB status and lay the ground for further research.

RESUMO A cetose pode trazer sérios prejuízos ao desempenho da vaca. Este estudo detectou alterações nos parâmetros metabólicos do sangue pré-parto para prever a cetose pós-parto que ocorre em vacas leiteiras. O escore de condição corporal (ECC) foi avaliado antes e após o parto. Foram coletadas amostras de sangue de 63 vacas entre 10 dias antes e 10 dias após o parto para medir os parâmetros metabólicos, incluindo ácido β-hidroxibutírico (BHBA), ácido graxo não esterificado (NEFA), glicose (GLU), bilirrubina total (TBIL), bilirrubina direta (DBIL), bilirrubina indireta (IBIL), proteína total (TP), albumina (ALB), globulina (GLO), alanina aminotransferase (ALT) e aspartato aminotransferase (AST). Houve uma incidência de cetose subclínica pós-parto de 42,25%. Em comparação com o pré-parto, o plasma, os níveis de BHBA, AST e NEFA aumentaram significativamente no pós-parto, e AST no pré-parto (R = 0,57) e NEFA (R = 0,45) mostraram uma correlação significativa positiva com cetose pós-parto. O nível plasmático de GLU diminuiu significativamente no pós-parto e foi negativamente correlacionado com a cetose de forma significativa (R = -0,21). A análise da curva característica de operação do receptor revelou BSC pré-parto <2,88 e AST plasmático pré-parto> 68,0 U / L, GLU <3,97mmol / L, NEFA> 0,27mmol / L e BHBA> 0,43mmol / L, indicando um alto risco de cetose subclínica pós-parto. Esses níveis podem ser usados como indicadores de risco para prever a ocorrência de cetose subclínica em vacas no pós-parto.

ABSTRACT Ketosis can seriously impair cow performance. This study detected changes in prepartum blood metabolic parameters for predicting postpartum ketosis occurrence in dairy cows. Body condition score (BCS) was assessed before and after delivery. Blood samples of 63 cows were collected from 10 days before calving to 10 days after calving to measure metabolic parameters including β-hydroxybutyric acid (BHBA), non-esterified fatty acid (NEFA), glucose (GLU), total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), total protein (TP), albumin (ALB), globulin (GLO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). There was a postpartum subclinical ketosis incidence of 42.25%. Compared with prepartum, plasma, levels of BHBA, AST, and NEFA significantly increased postpartum, and prepartum AST (R=0.57) and NEFA (R=0.45) showed a significant positive correlation with ketosis postpartum. Plasma GLU level significantly decreased postpartum and was significantly negatively correlated with ketosis (R=-0.21). Receiver operating characteristic curve analysis revealed prepartum BSC < 2.88, and prepartum plasma AST > 68.0 U/L, GLU < 3.97mmol/L, NEFA > 0.27mmol/L, and BHBA > 0.43mmol/L, indicating a high risk of subclinical ketosis postpartum. These levels can be used as risk indicators to predict the occurrence of subclinical ketosis in postpartum cows.

Abstract Background Snake venoms are complex mixtures of toxic proteins or peptides encoded by various gene families that function synergistically to incapacitate prey. In the present study, in order to unravel the proteomic repertoire of Deinagkistrodon acutus venom, some trace abundance components were analyzed. Methods Shotgun proteomic approach combined with shotgun nano-LC-ESI-MS/MS were employed to characterize the medically important D. acutus venom, after collected samples were enriched with the combinatorial peptide ligand library (CPLL). Results This avenue helped us find some trace components, undetected before, in D. acutus venom. The results indicated that D. acutus venom comprised 84 distinct proteins from 10 toxin families and 12 other proteins. These results are more than twice the number of venom components obtained from previous studies, which were only 29 distinct proteins obtained through RP-HPLC for the venom of the same species. The present results indicated that in D. acutus venom, the most abundant components (66.9%) included metalloproteinases, serine proteinases, and C-type lectin proteins; the medium abundant components (13%) comprised phospholipases A2 (PLA2) and 5’-nucleotidases and nucleases; whereas least abundant components (6%) were aminopeptidases, L-amino acid oxidases (LAAO), neurotoxins and disintegrins; and the trace components. The last were undetected before the use of conventional shotgun proteomics combined with shotgun nano-LC-ESI-MS/MS, such as cysteine-rich secretory proteins Da-CRPa, phospholipases B-like 1, phospholipases B (PLB), nerve growth factors (NGF), glutaminyl-peptide cyclortransferases (QC), and vascular non-inflammatory molecules 2 (VNN2). Conclusion These findings demonstrated that the CPLL enrichment method worked well in finding the trace toxin proteins in D. acutus venom, in contrast with the previous venomic characterization of D. acutus by conventional LC-MS/MS. In conclusion, this approach combined with the CPLL enrichment was effective for allowing us to explore the hidden D. acutus venomic profile and extended the list of potential venom toxins.

RESUMO Infecção com o vírus Hepatite E (HEV) é uma importante questão de saúde pública global. Infecções HEV são reconhecidas como doença zoológica. Acredita-se que suínos são o principal reservatório de HEV. Recentemente iaques, vacas, e gado amarelo foram reportados como novos reservatórios do HEV. Porém, não se sabe se outras espécies de gado e búfalo são sensíveis a infecção HEV. Para investigar a prevalência de infecção HEV em búfalos, foram realizados prova de imunoabsorção enzimática e polimerização em cadeia inversa ancorada em transcrição. Apenas um búfalo foi positivo para o anticorpo anti-HEV IgM (1/106, 0,94%), e nenhum foi positivo para o anticorpo anti-HEV IgG. Para nossa surpresa cinco (5/106, 4,72%) e três amostras de leite (3/40, 7,50%) de búfalos foram positivos para HEV RNA. Todas as estirpes de HEV isoladas de búfalos pertencem ao genótipo 4. Resultados indicam que búfalos podem ser um reservatório de HEV.

ABSTRACT Hepatitis E virus (HEV) infection is an important global public health issue. HEV infections are recognized as a zoonotic disease. Swine are believed to be the main reservoir of HEV. Recently, yaks, cows, and yellow cattle have been reported as new reservoirs of HEV. However, whether other species of cattle and buffaloes are sensitive to HEV infection is unknown. To investigate the prevalence of HEV infection in buffaloes, enzyme-linked immunosorbent assay (ELISA) and reverse transcription-nested polymerase chain reaction (RT-nPCR) were performed. Only one buffalo was positive to anti-HEV IgM antibody (1/106, 0.94%), and none were positive for anti-HEV IgG antibody. To our surprise, five serum (5/106, 4.72%) and three milk samples (3/40, 7.50%) from buffaloes were positive to HEV RNA. All strains of HEV isolated from buffaloes belong to genotype 4. Results indicate that buffaloes may be a new reservoir of HEV.