As one of the most important types of evidence at the scene of the crime, the rapid identification of the human bloodstain is of great significance to solve the criminal case. In this paper, the spectral data of different species of bloodstain samples including human, chicken and pig were acquired by using a hand-held near-infrared spectrometer. Then, the training models were established via convolutional neural network-support vector machine algorithm. Meanwhile, the traditional support vector machine, genetic algorithm-back propagation and random forest classification algorithms were also compared. The results showed that the prediction accuracy of convolutional neural network-support vector machine algorithm was the highest and the overall performance of the model was the best. The rapid detection method based on a handheld near-infrared spectrometer and convolutional neural network-support vector machine algorithm could identify the species of bloodstain efficiently, non-destructively, quickly and accurately and it provided a new technical reference for bloodstains detection and identification. crime case paper hand held nearinfrared near infrared Then networksupport network Meanwhile algorithmback back compared best efficiently nondestructively, nondestructively non destructively, destructively non-destructively

ABSTRACT This study was conducted to identify the key volatile compounds that characterize whether silage has deteriorated and to investigate the intrinsic link between the key compounds and silage odor. First, silages with different aerobic exposure durations were sampled, and sensory evaluation integrating aerobic stability monitoring was used to distinguish whether the silage had deteriorated. Subsequently, headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) was utilized to determine the relative content of the compounds. Using the relative content of the compounds in each silage as input, relative odor activity value (ROAV) calculations and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to determine the odor contribution of the compounds and the compounds with significant differences in relative content (based on variable importance for the projection, VIP) between the deteriorated and non-deteriorated silages. Next, the key compounds were identified by combining the conditions of average ROAV (aROAV) ≥ 1 and VIP > 1. Finally, the OPLS algorithm was used to analyze the intrinsic link of key compounds with the silage odor. The results showed that three out of 63 compounds—4-ethyl phenol, eugenol, and ethyl linoleate—were key compounds to characterize whether the silages deteriorated or not. In addition, ortho-guaiacol, 4-ethyl guaiacol, and 2-methoxy-4-vinyl phenol were the specific key compounds for deteriorated silage. Eugenol and ethyl linoleate were correlated with fruity, sour, and spicy odors. In addition, guaiacol, 4-ethylphenol, 4-ethyl guaiacol and 2-methoxy-4-vinyl phenol contributed to roasted, musty, and putrid odors. First sampled Subsequently solidphase solid phase chromatographymass chromatography mass HSSPMEGCMS HS SPME GC MS (HS-SPME-GC-MS input (ROAV OPLSDA DA (OPLS-DA based projection nondeteriorated non Next aROAV (aROAV Finally 6 compounds4ethyl compoundsethyl 4 eugenol linoleatewere not addition orthoguaiacol, orthoguaiacol ortho ortho-guaiacol 4ethyl 2methoxy4vinyl methoxyvinyl 2 methoxy vinyl fruity sour odors 4ethylphenol, 4ethylphenol ethylphenol ethylphenol, 4-ethylphenol roasted musty

Abstract Objective This study aimed to investigate the effect of Esketamine (ESK) on the Hypoxia/Reoxygenation (H/R) injury of cardiomyocytes by regulating TRPV1 and inhibiting the concentration of intracellular Ca2+. Methods The H/R injury model of H9c2 cardiomyocytes was established after 4h hypoxia and 6h reoxygenation. H9c2 cells were treated with different concentrations of ESK or TRPV1 agonist capsaicin (10 μM) or TRPV1 inhibitor capsazepine (1 μM). Cell viability was detected by CCK-8 method, and apoptosis by flow cytometry. Intracellular Ca2+ concentration was evaluated by Fluo-4 AM. LDH, MDA, SOD, and GSH-Px were detected with corresponding commercial kits. TRPV1 and p-TRPV1 proteins were detected by Western blot. Results After H/R, H9c2 cell viability decreased, apoptosis increased, intracellular Ca2+ concentration increased, LDH and MDA levels increased, SOD and GSH-Px levels decreased, and p-TRPV1 expression increased. ESK treatment rescued these changes induced by H/R. After up-regulating TRPV1, the protective effect of ESK on H/R injury of H9c2 cells was weakened, while down-regulating TRPV1 could further protect against H/R injury. Conclusion ESK alleviates H/R injury of cardiomyocytes by regulating TRPV1 expression and inhibiting intracellular Ca2+ concentration. (ESK HypoxiaReoxygenation Hypoxia Reoxygenation HR H R (H/R TRPV Ca2 Ca Hc c H9c h reoxygenation 10 μM 1 ( μM. . CCK8 CCK 8 CCK- method cytometry Fluo4 Fluo 4 Fluo- AM GSHPx GSH Px kits pTRPV1 pTRPV p p-TRPV blot decreased increased upregulating up weakened downregulating down

ABSTRACT Objective: The purpose of the present study was to examine the association of oxidative stress markers with sarcopenia in the general United States population under the age of 60. Methods: We used the National Health and Nutrition Examination Survey data from 2011‒2014 and performed Restricted Cubic Spline (RCS) plots, weighted multivariable logistic regression analysis to calculate ratio ratios and 95% Confidence Intervals, and subgroup analysis based on age, sex, hypertension, diabetes mellitus, and body mass index stratification to determine the association of markers of oxidative stress with the prevalence of sarcopenia. Results: The present analysis included a total of 8,782 participants. Firstly, the RCS plots showed a roughly L-shaped curve association of total bilirubin and serum iron with a prevalence of sarcopenia. Secondly, albumin was negatively and linearly associated with the risk of sarcopenia. Finally, with the increase in gamma-glutamyl transferase, the prevalence of sarcopenia showed a trend of first rising and then declining as a result of the iron increase. Conclusions: We demonstrated a nonlinear association between markers of oxidative stress and sarcopenia. The need to focus more on levels of oxidative stress in the body could provide better prevention strategies for sarcopenia. Objective 60 Methods 20112014 2011 2014 2011‒201 (RCS 95 Intervals sex hypertension mellitus Results 8782 8 782 8,78 participants Firstly Lshaped L shaped Secondly Finally gammaglutamyl gamma glutamyl transferase Conclusions 6 2011201 201 2011‒20 9 878 78 8,7 201120 20 2011‒2 87 7 8, 20112 2 2011‒

Abstract Objectives To evaluate the therapeutic effect of Saccharomyces boulardii supplementation on jaundice in premature infants undergoing phototherapy. Methods In this article, the authors reviewed 100 hospitalized jaundiced premature infants under 35 weeks of gestational age. All infants were assigned to a control group (n= 45) and a treatment group (n= 55) randomly. The infants in the treatment group received S. boulardii supplementation by undergoing phototherapy and the infants in the control group were only treated by phototherapy. The total serum bilirubin levels were detected before and at the end of phototherapy, and transcutaneous bilirubin levels were measured on the 1st, 4th, 8th and 15th day of treatment. The duration of jaundice resolution and phototherapy, stool frequency, and characteristics were compared after phototherapy. Results The duration of jaundice resolution and phototherapy were shortened. Total serum bilirubin level was lower than the control group at the end of phototherapy (p < 0.05). Transcutaneous bilirubin levels decreased more significantly on the 8th and 15th day of treatment (p < 0.05), while there were no significant differences on the post-treatment 1st and 4th day (p > 0.05). In addition, bowel movements including stool frequency and Bristol Stool Form Scale ratings of stools also improved after treatment. Conclusions S. boulardii in combination with phototherapy is effective and safe in reducing bilirubin levels and duration of phototherapy, accelerating jaundice resolution in premature infants with jaundice. The procedure also provided an ideal therapeutic effect of diarrhea induced by phototherapy to promote compliance and maternal-infant bonding. article 10 3 age n= n (n 45 55 randomly S st th shortened p 0.05. 005 0.05 . 0 05 0.05) 0.05, , posttreatment post addition maternalinfant maternal infant bonding 1 4 5 00 0.0 0.

ABSTRACT Introduction: Lower body perfusion (LBP) is a technique used to provide blood perfusion to distal organs and spinal cord during circulatory arrest. However, the effect of LBP on the prognosis of aortic arch surgery, especially on postoperative renal function, remains unclear. Methods: A total of 304 patients with acute type A aortic dissection who underwent total aortic arch replacement combined with frozen elephant trunk implantation between May 2016 and December 2021 were retrospectively analyzed. The patients were divided into LBP group (group L, n=85) and non-LBP group (group NL, n=219). Routine lower body circulatory arrest was applied during operation in group NL, and antegrade LBP combined was applied during operation in group L. Perioperative data were recorded. Propensity score matching was used for statistical analysis. Results: After propensity score matching, 85 pairs of patients were successfully matched. Two groups significantly differed in circulatory arrest time (six minutes vs. 30 minutes, P=0.000), cross-clamping time (101 minutes vs. 92 minutes, P=0.010), minimum nasopharyngeal temperature (29.4ºC vs. 27.2ºC, P=0.000), and highest lactate value during cardiopulmonary bypass (2.3 μmol/L vs. 4.1 μmol/L, P=0.000). Considering the postoperative indicators, the drainage volume (450 mL vs. 775 mL, P=0.000) and the incidence of level I acute kidney injury (23.5% vs. 32%, P=0.046) in group L was lower than those in group NL. Conclusion: LBP resulted as a safe and feasible approach in aortic arch surgery, as it could significantly shorten the circulatory arrest time, which might reduce the incidence of postoperative level I acute kidney injury. Introduction (LBP However surgery function unclear Methods 201 202 analyzed n=85 n85 n nonLBP non NL n=219. n219 n=219 . 219 n=219) recorded analysis Results 8 matched six vs 3 P=0.000, P0000 P P=0.000 , 0 000 crossclamping cross clamping 101 (10 9 P=0.010, P0010 P=0.010 010 P=0.010) 29.4ºC 294ºC ºC 29 4ºC 272ºC 27 2ºC 27.2ºC 2.3 23 2 (2. μmolL μmol 41 4 1 4. P=0.000. indicators 450 (45 77 23.5% 235 5 (23.5 32 32% P=0.046 P0046 046 Conclusion 20 n=8 n8 n21 n=21 21 P000 P=0.00 00 10 (1 P001 P=0.01 01 2. (2 45 (4 7 23.5 (23. P=0.04 P004 04 n= n2 n=2 P00 P=0.0 ( 23. (23 P0 P=0. P=0 P=

Abstract Objectives: This study aimed to compare progression-free survival, overall survival, clinical benefits, and adverse effects in postmenopausal women with hormone receptor-positive and HER2-negative breast cancer who received buparlisib plus fulvestrant against those of women who received dalpiciclib plus fulvestrant, considering ribociclib plus letrozole treatment as the reference standard. Methods: Women received buparlisib plus fulvestrant (BF cohort, n = 108), dalpiciclib plus fulvestrant (DF cohort, n = 132), or ribociclib plus letrozole (RL cohort, n = 150) until unacceptable toxicity was observed. Results: A total of 117 (89 %), 80 (74 %), and 84 (56 %) women in the BF, DF, and RL cohorts, respectively, had clinical benefits. After treatment, the clinical benefits for women and after 42 months of follow-up progression-free survival and overall survival were higher in the DF cohort than in the BF and RL cohorts (p < 0.05 for all). Neutropenia, vomiting, constipation, nausea, diarrhea, and anorexia were reported higher in women of the DF and BF cohorts than in women of the RL cohort. Leukopenia and increased levels of alanine aminotransferase and aspartate aminotransferase were reported to be higher in women in the RL cohort than in women in the DF and BF cohorts. Depression, anxiety, and increased levels of alanine aminotransferase and aspartate aminotransferase were reported to be higher in women in the BF cohort than in women in the DF and RL cohorts. Conclusions: Dalpiciclib plus fulvestrant is effective and comparatively safe in postmenopausal women with hormone receptor-positive and HER2-negative breast cancers. Dalpiciclib, buparlisib, fulvestrant, and ribociclib cause neutropenia, severe depression, adverse gastroenterological effects, and adverse hepatological effects, respectively. Objectives progressionfree progression free receptorpositive receptor positive HER2negative HERnegative HER2 negative HER standard Methods 108, 108 , 108) 132, 132 132) 150 observed Results 11 89 (8 %, % 8 74 (7 56 (5 respectively 4 followup follow up p 005 0 05 0.0 all. all . all) Neutropenia vomiting constipation nausea diarrhea Depression anxiety Conclusions cancers neutropenia depression 10 13 15 1 ( 7 5 00 0.

ABSTRACT Introduction: Lower body perfusion (LBP) is a technique used to provide blood perfusion to distal organs and spinal cord during circulatory arrest. However, the effect of LBP on the prognosis of aortic arch surgery, especially on postoperative renal function, remains unclear. Methods: A total of 304 patients with acute type A aortic dissection who underwent total aortic arch replacement combined with frozen elephant trunk implantation between May 2016 and December 2021 were retrospectively analyzed. The patients were divided into LBP group (group L, n=85) and non-LBP group (group NL, n=219). Routine lower body circulatory arrest was applied during operation in group NL, and antegrade LBP combined was applied during operation in group L. Perioperative data were recorded. Propensity score matching was used for statistical analysis. Results: After propensity score matching, 85 pairs of patients were successfully matched. Two groups significantly differed in circulatory arrest time (six minutes vs. 30 minutes, P=0.000), cross-clamping time (101 minutes vs. 92 minutes, P=0.010), minimum nasopharyngeal temperature (29.4ºC vs. 27.2ºC, P=0.000), and highest lactate value during cardiopulmonary bypass (2.3 μmol/L vs. 4.1 μmol/L, P=0.000). Considering the postoperative indicators, the drainage volume (450 mL vs. 775 mL, P=0.000) and the incidence of level I acute kidney injury (23.5% vs. 32%, P=0.046) in group L was lower than those in group NL. Conclusion: LBP resulted as a safe and feasible approach in aortic arch surgery, as it could significantly shorten the circulatory arrest time, which might reduce the incidence of postoperative level I acute kidney injury. Introduction (LBP However surgery function unclear Methods 201 202 analyzed n=85 n85 n nonLBP non NL n=219. n219 n=219 . 219 n=219) recorded analysis Results 8 matched six vs 3 P=0.000, P0000 P P=0.000 , 0 000 crossclamping cross clamping 101 (10 9 P=0.010, P0010 P=0.010 010 P=0.010) 29.4ºC 294ºC ºC 29 4ºC 272ºC 27 2ºC 27.2ºC 2.3 23 2 (2. μmolL μmol 41 4 1 4. P=0.000. indicators 450 (45 77 23.5% 235 5 (23.5 32 32% P=0.046 P0046 046 Conclusion 20 n=8 n8 n21 n=21 21 P000 P=0.00 00 10 (1 P001 P=0.01 01 2. (2 45 (4 7 23.5 (23. P=0.04 P004 04 n= n2 n=2 P00 P=0.0 ( 23. (23 P0 P=0. P=0 P=

Abstract The current developmental trend is to evaluate the quality of Yongchuan Xiuya tea rapidly. After spectrum pre-processing, near infrared spectroscopy (NIRS) coupled with synergy interval partial least squares (siPLS), principal component analysis (PCA) and back propagation-artificial neural network (BP-ANN) was applied to rapidly and non-destructively predict the quality of Yongchuan Xiuya tea. External Yongchuan Xiuya tea samples were used for the actual application of the proposed model. The best pre-processing method was multiple scattering correction coupled with second derivative, and the characteristic spectral regions selected by siPLS were 4381.5-4755.6 cm-1, 4759.5-5133.6 cm-1, 6266.6-6637.8 cm-1 and 7389.9-7760.2 cm-1. The cumulative contribution rate was 99.05% for the first three principal components of the characteristic spectra regions. The transfer function, root mean square error and determinant coefficient of the best BP-ANN prediction model were the tanh function, 0.384 and 0.977, respectively. The root mean square error and determinant coefficient of the external 10 Yongchuan Xiuya tea samples were 0.406 and 0.969, respectively. These results showed that NIRS combined with BP-ANN algorithm can be used to evaluate the quality of Yongchuan Xiuya tea rapidly and accurately.

Abstract Anthocyanin/polyvinyl alcohol/chitosan composite membrane was prepared by solution casting method using chitosan (CS) and polyvinyl alcohol (PVA) as matrix and natural pigment anthocyanin as filler. The structures of the composite films were characterized by Uv-visible reflectance spectrum (UV-VIS), Fourier infrared spectroscopy (FT-IR) and the scanning electron microscopy (SEM). The effects of adding anthocyanins on the physical properties, water resistance, degradation, antibacterial properties and pH responsiveness of the films were investigated. The experimental results showed that the prepared anthocyanin/polyvinyl alcohol/chitosan composite indicator films show color changes from pink to purple to blue and then to yellow-green when the pH value ranges from 2 to 14. The total color difference ΔE value is greater than 12. The antibacterial properties and other properties of the composite film can enhance by adding anthocyanin. The tensile strength of anthocyanin/polyvinyl alcohol/chitosan film is more than 70 MPa, which is larger than that of polyvinyl film and polyvinyl alcohol/chitosanfilm. The film has excellent physical, antibacterial properties and obvious pH response performance, making it ideal for food packaging.

SUMMARY: Skeletal muscle injury is an acute inflammatory condition caused by an inflammatory response. To reduce inflammatory cell infiltration and relieve skeletal muscle injury, efficient treatment is urgently needed. Nitric oxide is a free radical molecule reported to have anti-inflammatory effects. In this study, we showed that NO could inhibit the inflammatory response of C2C12 cells in vitro and protect rat skeletal muscle injury from notexin in vivo. NO synthase inhibitor (L-NG-Nitroarginine Methyl Este?L-NAME) and NO donor (sodium nitroprusside dehydrate ?SNP) were used to explore the vital role of lipopolysaccharides (LPSs) in LPS-stimulated C2C12 myoblasts.The expression of IL-18 and IL-1b was upregulated by L-NAME and downregulated by SNP, as indicated by the ELISA results. NO can reduce ASC, Caspase-1, and NLRP3 mRNA and protein levels. Furthermore, NO was detected in the rat model. The results of immunohistochemical staining showed that the production of DMD decreased. We conducted qRT-PCR and western blotting to detect the expression of Jo-1, Mi-2, TLR2, and TLR4 on day 6 post injury following treatment with L-NAME and SNP. The expression of Jo-1, Mi-2, TLR2, and TLR4 was upregulated by L-NAME and significantly reversed by SNP. NO can alleviate C2C12 cell inflammatory responses and protect rat skeletal muscle injury from notexin.

RESUMEN: La lesión del músculo esquelético es una afección inflamatoria aguda causada por una respuesta inflamatoria. Para reducir la infiltración de células inflamatorias y aliviar la lesión del músculo esquelético es necesario un tratamiento eficaz. El óxido nítrico es una molécula de radicales libres que tiene efectos antiinflamatorios. En este estudio, demostramos que el ON podría inhibir la respuesta inflamatoria de las células C2C12 in vitro y proteger la lesión del músculo esquelético de rata de la notexina in vivo. El inhibidor de ON sintasa (L-NG-nitroarginina metil este, L-NAME) y el donante de ON (nitroprusiato de sodio deshidratado, SNP) se utilizaron para explorar el papel vital de los lipopolisacáridos (LPS) en los mioblastos C2C12 estimulados por LPS. La expresión de IL- 18 e IL-1b fue regulada positivamente por L-NAME y regulada negativamente por SNP, como indican los resultados de ELISA. El ON puede reducir los niveles de proteína y ARNm de ASC, Caspasa-1 y NLRP3. Además, se detectó ON en el modelo de rata. Los resultados de la tinción inmunohistoquímica mostraron que disminuyó la producción de DMD. Realizamos qRT-PCR y transferencia Western para detectar la expresión de Jo-1, Mi-2, TLR2 y TLR4 el día 6 después de la lesión después del tratamiento con L-NAME y SNP. La expresión de Jo-1, Mi-2, TLR2 y TLR4 fue regulada positivamente por L- NAME y significativamente revertida por SNP. El ON puede aliviar las respuestas inflamatorias de las células C2C12 en ratas, y proteger la lesión del músculo esquelético de la notexina.

ABSTRACT Purpose: The active melanocytes in the skin were affected by hormones and ultraviolet (UV) irradiation. Licorice zinc has a whitening effect, which may have a prominent potential in the treatment of pigmented skin disease. Methods: Modeling chloasma Balb/c mice by daily progesterone injection (15 mg/kg) and ultraviolet B (UVB) irradiation (λ = 312 nm, 2 h/day) for 30 days. Then, mice were given 0.65, 1.3, and 2.6 (g/kg) of licorice zinc and tranexamic acid 250 mg daily by oral administration for 14 days, respectively. Hematoxylin and eosin and Fontana-Masson staining, and Western blotting (WB) were performed to test the inhibitory of melanogenesis and activation of c-Jun-N-terminal (JNK)/p38 mitogen-activated protein kinases (MAPK) for licorice zinc. Melanogenesis was induced by α-melanocyte-stimulating hormone in vitro. Cell counting kit-8, melanin content determination, and WB were performed to verify the inhibitory effect of licorice zinc on melanogenesis. Results: The present study showed that licorice zinc decreased melanin formation, cutaneous tissue injury, and the phosphorylation of JNK and P38MAPK, which was caused by UVB irradiation in vivo. In vitro, licorice zinc showed opposite effects from JNK/p38 activator. Meanwhile, tyrosinase-related protein-1, tyrosinase, and microphthalmia-associated transcription factor were decreased too. Conclusions: Licorice zinc induced a decrease in melanin synthesis by inhibiting the JNK and the P38MAPK signaling pathway, suggesting licorice zinc is a potential agent of anti-chloasma. Purpose UV (UV disease Methods Balbc Balb c 15 (1 mg/kg mgkg kg (UVB λ 31 nm h/day hday h day 3 days Then 065 0 65 0.65 13 1 1.3 26 6 2. g/kg gkg g (g/kg 25 respectively FontanaMasson Fontana Masson staining (WB cJunNterminal Jun N terminal JNKp38 JNKp /p38 p38 p (JNK)/p3 mitogenactivated mitogen activated MAPK (MAPK αmelanocytestimulating α melanocyte stimulating vitro kit8, kit8 kit 8, 8 kit-8 determination Results formation injury PMAPK P vivo JNK/p3 activator Meanwhile tyrosinaserelated tyrosinase related protein1, protein1 1, protein-1 microphthalmiaassociated microphthalmia associated too Conclusions pathway antichloasma. antichloasma anti chloasma. anti-chloasma ( 06 0.6 1. JNKp3 /p3 p3 (JNK)/p kit- JNK/p protein- 0. /p

Abstract Owing to the high mineralization and high treatment cost, Ordovician limestone water is often regarded as a mine wastewater. In order to make rational use of mine water with high mineralization and turn waste into treasure. In this work, the natural water quality of Ordovician mine water in the Weibei coalfield had been tested, and the suspended matter and trace elements of Ordovician mine water in the selected deep detained area had been further tested. As a contrast, the water quality of Ordovician mine water after heating and concentration had been tested. The mechanical and hydraulic parameters of concentrated mine water-loess and concentrated mine water-cement slurry had been tested and compared with conventional slurry. The results showed characteristics of deep detained Ordovician limestone mine water is high salinity, certain suspended matters, limited special material and high permanent hardness. However, compressive strength of loess samples increased, while the permeability reduced. The initial setting-time of the modified material was short, while it showed an increased compressive strength. In practical terms, the quantity of grouting produced in engineering applications can be reduced by 16%, whereas the discharge of high-mineralized mine water can be decreased by about 40,000 m3/a.

Abstract To select the optimal condition of 1064 nm Q-switched Nd:YAG laser irradiation and study the molecular mechanism of laser irradiation for skin rejuvenation. Sixty male, 6-week-old SKH-1 hairless mice were equally divided into 6 groups: 3d, 7d, 21d, 28d, 42d and 56d of 1064 nm laser irradiation at both 0 J/cm2 (left region) and 1.5 J/cm2 (right region) fluence. The basic skin performances were analysed via quantifying hydroxyproline content and skin hydration in the dermis and epidermis. The Collagen I, MMP2 and TIMP1, as well as ERK 1/2, p38MAPK, JNK and ERK5 signaling pathways levels were detected by qRT-PCR. Laser irradiation led to a marked increase in hydroxyproline content and skin hydration in the dermis and epidermis compared to the non-irradiated area. After laser irradiation, collagen I was markedly up-regulated, MMP2 was significantly decreased and TIMP1 was unchanged. Moreover, qRT-PCR results revealed ERK 1/2 and p38MAPK signaling pathways were activated after laser irradiation. we are firstly proves that 1064 nm laser irradiation can inhibit collagen degradation by stimulating the activation of ERK1/2 and p38MAPK signaling pathways. Additionally, the condition of laser irradiation lasting 21d at fluence of 1.5 J/cm2 displays the best effect of skin rejuvenation in our study.

Abstract Inflammation-related immune responses and bone metabolism lead to extensive tooth loss in periodontitis. Objective: This study aims to investigate the effect of peroxisome proliferator-activated receptor (PPAR) alpha agonist anti-inflammatory treatment in vitro and in ligature-induced experimental periodontitis in vivo . Methodology: Splenocytes were isolated from C57BL/6J mice and cultured for 48 hours under the following conditions: control, P. gingivalis lipopolysaccharide (LPS) (1 µg/ml); experimental, LPS (1 µg/ml) + PPARα agonist (fenofibrate) at 1, 10, 50, 100 µM. MRNA and secreted protein levels of TNF-α expression were detected by RT-qPCR and ELISA, respectively. Silk ligatures (7-0) were tied around maxillary second molars of C57BL/6J mice for two weeks. Optimized doses of fenofibrate (50 µM) and vehicle control were injected into the contralateral side of the palatal gingiva on days three, six, and nine. At day 14, bone resorption, osteoclastogenesis, and gingival mRNA expression levels of TNF-α, IL-1β, IL-6, and RANKL/OPG were measured by micro-computed tomography, Tartrate-resistant acid phosphatase (TRAP) staining, and Real-time quantitative PCR, respectively. Results: TNF-α expression in cultured spleen cells were significantly increased in the presence of LPS, when compared with the control group, and significantly reduced by fenofibrate treatment in a dose-dependent manner from 1-100 µM (p<0.05). Gingival mRNA levels of TNF-α, IL-1β, IL-6, and the ratio of RANKL/OPG, were significantly decreased after injection of fenofibrate, when compared to the control side (p<0.05). Periodontal bone loss and TRAP positive cell formation were significantly decreased on the side with an injection of fenofibrate, as compared to the control side (p<0.05). Conclusions: An anti-inflammatory treatment, PPARα agonist, inhibited inflammation and periodontal bone loss in ligature-induced experimental periodontitis.