Abstract Introduction: Propofol is a widely used anesthetic and its dose is closely related to aging. Telomere length (TL) is a unique heritable trait, and emerging as a biomarker of aging, health and disease. Telomerase RNA component (TERC) plays an important role in maintaining TL. We proposed a hypothesis that propofol dose in general anesthesia can be predicted by measuring TL before operation, which greatly reduced the risk of anesthesia, especially the elderly. Methods: The association between the propofol dose in anesthesia induction and: TL in the DNA of peripheral blood leukocytes; body weight; sex; difference of the Bispectral Index (BIS) before and after anesthesia induction in patients was evaluated by multivariable linear regression analyses. The mutation at the 5’end or 3’end of TERC was detected. We recruited 100 patients of elective surgery. Results: We found that propofol dose in anesthesia induction was clearly correlated significantly with TL (r = 0.78, p < 0.001), body weight (r = 0.84, p = 0.004), sex (r = 0.83, p= 0.84, p = 0.004), sex (r = 0.83, p = 0.004), and difference of BIS before and after anesthesia induction (r = 0.85, p = 0.029). By comparing the absolute values of standardized regression coefficients (0.58, 0.21, 0.19, and 0.12) of the four variables, it can be seen that TL contributes the most to the propofol dose in anesthesia induction. However, the mutation at the 5’ end or 3’ end of TERC was not found. Conclusions: These findings provide preliminary evidence that the propofol dose in anesthesia induction was clearly correlated with genetically determined TL. TL may be a promising predictor of the propofol dose, which is beneficial to improve the safety of anesthesia and reduce perioperative complications. Introduction aging (TL trait disease (TERC operation elderly Methods leukocytes (BIS analyses 5end 5 3end 3 detected 10 surgery Results r 078 0 78 0.78 0.001, 0001 0.001 , 001 0.001) 084 84 0.84 0.004, 0004 0.004 004 0.004) 083 83 0.83 085 85 0.85 0.029. 0029 0.029 . 029 0.029) 0.58, 058 58 (0.58 021 21 0.21 019 19 0.19 0.12 012 12 variables However Conclusions complications 1 07 7 0.7 000 0.00 00 08 8 0.8 002 0.02 02 0.58 05 (0.5 2 0.2 01 0.1 0. 0.0 0.5 (0. (0 (

ABSTRACT Objectives To compare the dusting efficiency and safety with basketing for treating renal stones ≤ 2 cm during flexible ureteroscopy (fURS). Materials and methods This study included 218 patients with renal stones ≤ 2 cm treated with fURS. Among them, 106 patients underwent dusting, and 112 patients underwent fragmentation with basket extraction. All patients were followed up for 3 months postoperatively. The operating time, lasing time, stone-free rate (SFR) and complication rate were compared. Results The mean stone size in the dusting group was 1.3 cm, whereas 1.4 cm in the basketing group. The mean operative time was significantly lower in the dusting group than in the basketing group (43.1±11.7 minutes VS 60.5±13.4 minutes, P <0.05), but the lasing time was significantly longer for the dusting group than for the basketing group (17.7±3.9 minutes VS 14.1±3.6 minutes, P <0.05). SFR was significantly higher in the basketing group immediately after the operation and follow-up after 1 month (76.8% vs 55.7%, P= 0.001 and 88.4% vs 78.3%, P = 0.045). However, the SFR was similar for both groups (88.8% in the dusting group vs. 90.2% in the basketing group) after 3 months postoperatively. There was no statistical difference in the complication rates between the two groups. Conclusions Dusting has advantages in shortening the operation time and reducing the operation cost, but the lasing time was longer compared with the basketing. Although there is no difference in long-term effect, basketing is superior to dusting in terms of short-term SFR. Moreover, dusting should be avoided in some special cases and basketing a better choice. Both techniques are effective for the treatment of renal stones ≤ 2 cm and choice depends on patient demographic and stone characteristics. fURS . (fURS) 21 them 10 11 extraction postoperatively stonefree free (SFR 13 1. 14 4 43.1±11.7 431117 43 7 (43.1±11. 605134 60 5 60.5±13. <0.05, 005 <0.05 , 0 05 <0.05) 17.7±3.9 17739 17 9 (17.7±3. 14136 6 14.1±3. <0.05. followup follow 76.8% 768 76 8 (76.8 557 55 55.7% 0001 001 0.00 884 88 88.4 783 78 78.3% 0.045. 0045 0.045 045 0.045) However 88.8% 888 (88.8 902 90 90.2 cost longterm long term effect shortterm short Moreover characteristics (fURS 43.1±11. 43111 (43.1±11 60513 60.5±13 00 <0.0 17.7±3. 1773 (17.7±3 1413 14.1±3 76.8 (76. 55.7 000 0.0 88. 78.3 004 0.04 04 88.8 (88. 90. 43.1±11 4311 (43.1±1 6051 60.5±1 <0. 17.7±3 177 (17.7± 141 14.1± 76. (76 55. 0. 78. (88 43.1±1 431 (43.1± 605 60.5± <0 17.7± (17.7 14.1 (7 (8 43.1± (43.1 60.5 < 17.7 (17. 14. ( 43.1 (43. 60. 17. (17 43. (43 (1 (4

Abstract Objective: To investigate the optimal timing of initial intravenous immunoglobulin (IVIG) treatment in Kawasaki disease (KD) patients. Methods: KD patients were classified as the early group (day 1-4), conventional group (day 5-7), conventional group (day 8-10), and late group (after day 10). Differences among the groups were analyzed by ANOVA and Chi-square analysis. Predictors of IVIG resistance and the optimal cut-off value were determined by multiple logistic regression analyses and receiver operating characteristic (ROC) curve analysis. Results: There were no significant differences in IVIG resistance among the 4 groups (p = 0.335). The sensitivity analysis also confirmed no difference in the IVIG resistance between those who started the initial IVIG ≤ day 7 of illness and those who received IVIG >day 7 of illness (p = 0.761). In addition, patients who received IVIG administration more than 7 days from the onset had a higher proportion of coronary artery abnormalities (p = 0.034) and longer length of hospitalization (p = 0.033) than those who started IVIG administration less than 7 days. The optimal cut-off value of initial IVIG administration time for predicting IVIG resistance was >7 days, with a sensitivity of 75.25% and specificity of 82.41%. Conclusions: IVIG therapy within 7 days of illness is found to be more effective for reducing the risk of coronary artery abnormalities than those who received IVIG >day 7 of illness. IVIG treatment within the 7 days of illness seems to be the optimal therapeutic window of IVIG. However, further prospective studies with long-term follow-up are required. Objective (IVIG (KD Methods 14, 14 1 , 1-4) 57, 57 5 5-7) 810, 810 8 10 8-10) after 10. . 10) Chisquare Chi square cutoff cut off ROC (ROC Results p 0.335. 0335 0.335 0 335 0.335) 0.761. 0761 0.761 761 0.761) addition 0.034 0034 034 0.033 0033 033 > 7525 75 25 75.25 8241 82 41 82.41% Conclusions However longterm long term followup follow up required 1-4 5-7 81 8-10 0.33 33 076 0.76 76 0.03 003 03 752 2 75.2 824 82.41 1- 5- 8-1 0.3 3 07 0.7 0.0 00 75. 82.4 8- 0. 82.

Abstract Long non-coding RNA AK001796 was initially identified altered in lung cancer. Recent research showed it could participate in the prognosis of hepatocellular carcinoma (HCC). However, the general biological role of AK001796 and its underlying mechanisms in HCC remain unclear. Here we demonstrated that the expression level of AK001796 in HCC tissues and cell lines was up-regulated. Silencing AK001796 suppressed the proliferation ability of HCC cells. Through dual luciferase reporter assays and loss/gain of functions studies, we identified that AK001796 could bind to miR-150, a star microRNA, promoting HCC proliferation. Furthermore, it was reported that growth factor receptor binding protein 2-associated binder 1 (GAB1) is a target gene of miR-150. Owing to AK001796 being a decoy for miR-150 and binding the same putative sites of miR-150 as GAB1, we presented that inhibition of miR-150 in AK001796 silencing cells reversed the reduction in GAB1. Subsequently, our findings demonstrated that silencing AK001796 can impair phospho-ERK1/2 and phospho-AKT. In conclusion, our investigation revealed that AK001796 promoted proliferation by enhancing phospho-ERK1/2 and phospho-AKT through AK001796/miR-150/GAB1 axis in HCC. These results provided further evidence for the critical roles of AK001796 accumulating HCC and suggested that AK001796 might act as an HCC biomarker in clinical treatment. noncoding non coding AK AK00179 cancer . (HCC) However unclear upregulated. upregulated up regulated. regulated up-regulated lossgain loss gain studies miR150, miR150 miR 150, 150 microRNA Furthermore 2associated associated 2 GAB1 GAB (GAB1 miR150. 150. miR-15 Subsequently phosphoERK1/2 phosphoERK12 phosphoERK phospho ERK1/2 ERK1 ERK phospho-ERK1/ phosphoAKT. phosphoAKT AKT. AKT conclusion AK001796/miR150/GAB1 AK001796miR150GAB1 AKmiRGAB AK001796/miR 150/GAB1 AK001796/miR-150/GAB treatment AK0017 (HCC miR15 15 (GAB miR-1 phosphoERK1 phosphoERK1/ ERK12 ERK1/ phospho-ERK1 AK001796/miR150/GAB AK001796miR150GAB AK001796miR AKmiR 150GAB1 150/GAB AK001 miR1 miR- phospho-ERK 150GAB AK00 AK0

Abstract Background: Bivalent freeze-dried neurotoxic (FN) antivenom has been the primary treatment since the 1980s for Taiwan cobra (Naja atra) envenomation in Taiwan. However, envenomation-related wound necrosis is a significant problem after cobra snakebites. In the present study, we analyzed the changes in serum venom concentration before and after antivenom administration to discover their clinical implications and the surgical treatment options for wound necrosis. Methods: The patients were divided into limb swelling and wound necrosis groups. The clinical outcome was that swelling started to subside 12 hours after antivenom treatment in the first group. Serum venom concentrations before and after using antivenoms were measured to assess the antivenom's ability to neutralize the circulating cobra venom. The venom levels in wound wet dressing gauzes, blister fluids, and debrided tissues were also investigated to determine their clinical significance. We also observed the evolutional changes of wound necrosis and chose a better wound debridement timing. Results: We prospectively enrolled 15 Taiwan cobra snakebite patients. Males accounted for most of this study population (n = 11, 73%). The wound necrosis group received more antivenom doses than the limb swelling group (4; IQR:2-6 vs 1; IQR:1-2, p = 0.05), and less records of serum venom concentrations changed before/after antivenom use (p = 0.0079). The necrotic wound site may release venom into circulation and cause more severe envenomation symptoms. Antivenom can efficiently diminish limb swelling in cobra bite patients. However, antivenom cannot reduce wound necrosis. Patients with early debridement of wound necrosis had a better limb outcome, while late or without debridement may have long-term hospital stay and distal limb morbidity. Conclusions: Antivenom can efficiently eliminate the circulating cobra venom in limb swelling patients without wound necrosis. Early debridement of the bite site wound and wet dressing management are suggestions for preventing extended tissue necrosis and hospital stay. Background freezedried freeze dried FN (FN s Naja atra However envenomationrelated related snakebites Methods groups 1 gauzes fluids significance timing Results n 11 73%. 73 73% . 73%) 4 (4 IQR26 IQR 2 6 IQR:2- IQR12, IQR12 2, IQR:1-2 0.05, 005 0.05 , 0 05 0.05) beforeafter 0.0079. 00079 0.0079 0079 0.0079) symptoms longterm long term morbidity Conclusions 7 ( IQR2 IQR:2 IQR1 IQR:1- 00 0.0 0007 0.007 007 IQR: IQR:1 0. 000 0.00

RESUMEN Introducción: La gimnasia aeróbica es un deporte que envuelve un sentido de belleza, ritmo y danza, con alta expresividad artística, características que favorecieron una fuerte adhesión de la población. Se cree que el ejercicio específico de la flexibilidad puede agregar un mayor impacto visual a sus movimientos artísticos, elevando el desempeño competitivo. Objetivo: Verificar los impactos del ejercicio de flexibilidad de los miembros inferiores en el desempeño de atletas de aeróbica. Métodos: Seis atletas de aeróbic fueron seleccionados para realizar un experimento de 8 semanas, adoptando la metodología PNF para el entrenamiento. Los impactos en la flexibilidad de los miembros inferiores fueron medidos antes y después del experimento, estos datos fueron analizados y estudiados estadísticamente. Resultados: Los resultados del salto de longitud en el índice de la calidad física de los atletas aumentaron en 0,1209 m; el valor añadido en la prueba de dos minutos y 10 m de espalda fue de 1,46. Los índices de rendimiento de movimiento mostraron aumentos expresivos: de flexión tuvo un aumento de 2,62; apoyo en ángulo recto aumentó 0,78; salto de pierna dividida aumentó 1,95; salto de pie con los ojos cerrados y el índice de calidad de equilibrio de los pies fue de 4,63. Conclusión: El ejercicio de flexibilidad de los miembros inferiores puede influir positivamente en el rendimiento de los atletas de gimnasia aeróbica. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción belleza danza artística población artísticos competitivo Objetivo Métodos semanas entrenamiento estadísticamente Resultados 01209 0 1209 0,120 1 146 46 1,46 expresivos 2,62 262 2 62 0,78 078 78 1,95 195 95 463 4 63 4,63 Conclusión II tratamiento 0120 120 0,12 14 1,4 2,6 26 6 0,7 07 7 1,9 19 9 4,6 012 12 0,1 1, 2, 0, 4, 01

ABSTRACT Introduction: Aerobic gymnastics is a sport that involves a sense of beauty, rhythm and dance, with high artistic expressiveness, characteristics that have favored a strong population adherence. It is believed that the specific flexibility exercise can add a greater visual impact to their artistic movements, raising their competitive performance. Objective: Verify the impacts of lower limb flexibility exercise on the performance of aerobics athletes. Methods: Six aerobics athletes were selected to perform an 8-week experiment, adopting the PNF methodology for training. The impacts on lower limb flexibility were measured before and after the experiment, these data were analyzed and studied statistically. Results: The results of the long jump in the physical quality index of the athletes increased by 0.1209 m; the added value in the two-minute, 10-m backstroke test was 1.46. The movement performance indexes showed expressive increases: flexion increased by 2.62; right angle support increased by 0.78; split leg jump increased by 1.95; standing jump with eyes closed and the foot balance quality index was 4.63. Conclusion: Lower limb flexibility exercise can positively impact the performance of aerobic gymnastics athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction beauty dance expressiveness adherence movements Objective Methods 8week week 8 experiment training statistically Results 01209 0 1209 0.120 m twominute, twominute two minute, minute two-minute 10m 10 146 1 46 1.46 increases 2.62 262 2 62 0.78 078 78 1.95 195 95 463 4 63 4.63 Conclusion II outcomes 0120 120 0.12 14 1.4 2.6 26 6 0.7 07 7 1.9 19 9 4.6 012 12 0.1 1. 2. 0. 4. 01

RESUMO Introdução: A ginástica aeróbica é um esporte que envolve senso de beleza, ritmo e dança, com alta expressividade artística, características que favoreceram uma forte adesão populacional. Acredita-se que o exercício específico de flexibilidade possa adicionar um maior impacto visual aos seus movimentos artísticos, elevando o desempenho competitivo. Objetivo: Verificar os impactos do exercício de flexibilidade dos membros inferiores sobre o desempenho dos atletas de aeróbica. Métodos: Seis atletas de aeróbica foram selecionados para realizar uma experiência de 8 semanas, adotando a metodologia PNF para o treinamento. Os impactos sobre a flexibilidade dos membros inferiores foram aferidos antes e depois do experimento, esses dados foram analisados e estudados estatisticamente. Resultados: Os resultados do salto em distância no índice de qualidade física dos atletas aumentaram em 0,1209 m; o valor agregado no teste de dois minutos e 10 m de recuo foi de 1,46. Os índices de desempenho do movimento apresentaram aumentos expressivos: de flexão teve um aumento de 2,62; apoio em ângulo reto aumentou 0,78; salto de pernas divididas aumentou 1,95; salto de pé com os olhos fechados e o índice de qualidade de equilíbrio dos pés foi de 4,63. Conclusão: O exercício de flexibilidade para membros inferiores pode impactar positivamente o desempenho dos atletas de ginástica aeróbica. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução beleza dança artística populacional Acreditase Acredita se artísticos competitivo Objetivo Métodos semanas treinamento experimento estatisticamente Resultados 01209 0 1209 0,120 1 146 46 1,46 expressivos 2,62 262 2 62 0,78 078 78 1,95 195 95 463 4 63 4,63 Conclusão II tratamento 0120 120 0,12 14 1,4 2,6 26 6 0,7 07 7 1,9 19 9 4,6 012 12 0,1 1, 2, 0, 4, 01

Abstract Background Gout is a common inflammatory arthritis caused by increased serum uric acid levels. Untreated or insufficiently treated gout can lead to deposition of monosodium urate crystals in joints, cartilage, and kidneys. Although Tongfengding capsules, a Chinese patent medicine, have long been used to treat gout, their effects and safety have not been reviewed systematically. This study evaluated its efficacy and safety for gout in adults. Methods Randomized controlled trials involving Tongfengding capsule for gout in adults were searched from PubMed, EMBASE, Cochrane Central Register of Controlled Trials, CBM, CNKI, and VIP databases, and analyzed using the Cochrane Handbook criteria. The primary outcome measures were the total effective rate. The secondary outcome measures including the blood uric acid (BUA), 24-h urinary total protein (24-h UTP), blood urea nitrogen (BUN), interleukin (IL)-6, IL-8, tumor necrosis factor-alpha (TNF-α) and adverse effects. The risk of bias was evaluated in all included studies. RevMan ver. 5.3.5 and GRADE profiler was used for data analysis and assessing the quality of evidence, respectively. Results Six studies (n = 607 Chinese participants) were included. Tongfengding capsules plus conventional treatment significantly increased the total effective rate (RR 1.21, 95% CI 1.11–1.33), while reducing the BUA (MD − 66.05 μmol/L, 95% CI − 81.26 to − 50.84), 24-h UTP (MD − 0.83 g/24 h, 95% CI − 0.96 to − 0.70), BUN (MD − 0.90 mmol/L, 95% CI − 1.60 to − 0.20), IL-6 (MD − 6.99 ng/L, 95% CI − 13.22 to − 0.75), IL-8 (MD − 12.17 ng/L, 95% CI − 18.07 to − 6.27), TNF-α (MD − 8.50 ng/L, 95% CI − 15.50 to − 1.51), and adverse effects (RR 0.21, 95% CI 0.04–0.95). Conclusion Tongfengding capsules plus conventional treatment is safe and beneficial for adults with gout compared with conventional treatment. levels joints cartilage kidneys medicine systematically PubMed EMBASE Trials CBM CNKI databases criteria BUA, , (BUA) 24h h 24 UTP, UTP) BUN, (BUN) IL6, IL6 IL 6, 6 (IL)-6 IL8, IL8 8, 8 factoralpha factor alpha TNFα TNF α (TNF-α ver 535 5 3 5.3. evidence respectively n 60 participants RR 121 1 21 1.21 95 1.11–1.33, 111133 1.11–1.33 11 33 1.11–1.33) MD 6605 66 05 66.0 μmolL μmol L μmol/L 8126 81 26 81.2 50.84, 5084 50.84 50 84 50.84) 083 0 83 0.8 g24 g g/2 096 96 0.9 0.70, 070 0.70 70 0.70) 090 90 mmolL mmol mmol/L 160 1.6 0.20, 020 0.20 20 0.20) IL- 699 99 6.9 ngL ng ng/L 1322 13 22 13.2 0.75, 075 0.75 75 0.75) 1217 12 17 12.1 1807 18 07 18.0 6.27, 627 6.27 27 6.27) 850 8.5 1550 15 15.5 1.51, 151 1.51 51 1.51) 021 0.21 0.04–0.95. 004095 0.04–0.95 . 04 0.04–0.95) (BUA 2 (BUN (IL)- 53 5.3 1.2 9 11113 1.11–1.3 660 66. 812 81. 508 50.8 08 0. g2 g/ 09 0.7 7 16 1. 02 0.2 69 6. 132 13. 12. 180 18. 62 6.2 85 8. 155 15. 1.5 00409 0.04–0.9 (IL) 5. 1111 1.11–1. 50. 0040 0.04–0. (IL 111 1.11–1 004 0.04–0 1.11– 00 0.04– 1.11 0.04 1.1 0.0

ABSTRACT Introduction: Thoracic aortic aneurysm is a potentially fatal disease with a strong genetic contribution. The dysfunction of vascular smooth muscle cells (VSMCs) contributes to the formation of this aneurysm. Although previous studies suggested that long non-coding ribonucleic acid (RNA) hypoxia inducible factor 1 α-antisense RNA 1 (HIF1A-AS1) exerted a vital role in the progression and pathogenesis of thoracic aortic aneurysm, we managed to find a new regulatory mechanism of HIF1A-AS1 in VSMCs via transcriptomics. Methods: Cell viability was detected by the cell counting kit-8 assay. Cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide double staining. Transwell migration assay and wound healing assay were performed to check the migration ability of HIF1A-AS1 on VSMCs. The NextSeq XTen system (Illumina) was used to collect RNA sequencing data. Lastly, reverse transcription-quantitative polymerase chain reaction confirmed the veracity and reliability of RNA-sequencing results. Results: We observed that overexpressing HIF1A-AS1 successfully promoted apoptosis, significantly altered cell cycle distribution, and greatly attenuated migration in VSMCs, further highlighting the robust promoting effects of HIF1A-AS1 to thoracic aortic aneurysm. Moreover, transcriptomics was implemented to uncover its underlying mechanism. A total of 175 differently expressed genes were identified, with some of them enriched in apoptosis, migration, and cell cycle-related pathways. Intriguingly, some differently expressed genes were noted in vascular development or coagulation function pathways. Conclusion: We suggest that HIF1A-AS1 mediated the progression of thoracic aortic aneurysm by not only regulating the function of VSMCs, but also altering vascular development or coagulation function. Introduction contribution (VSMCs noncoding non coding (RNA αantisense α antisense HIF1AAS1 HIFAAS HIF1A AS1 HIF AS (HIF1A-AS1 HIF1A-AS Methods kit8 kit 8 kit- Vfluorescein V fluorescein isothiocyanatepropidium isothiocyanate propidium staining Illumina (Illumina data Lastly transcriptionquantitative transcription quantitative RNAsequencing results Results distribution Moreover 17 identified cyclerelated related pathways Intriguingly Conclusion AAS HIF1AAS HIFA (HIF1A-AS

Abstract Adaptation of photoperiod-sensitive japonica rice varieties from long-day (LD) to short-day (SD) conditions is impeded by their extremely early flowering in response to photoperiod change, but the genetic factor underlying this is still elusive. We detected mutations in Hd1 in Zhenjing2400 through gene mapping and sequencing analysis. Genome resequencing of the varieties Zhenjing2400 and Jiahe218 identified single nucleotide polymorphisms (SNPs) in the other flowering-related genes Ehd1, SDG725, OsCOL15, DTH2, and DTH7. We constructed recombinant inbred lines (RILs) derived from a cross between Zhenjing2400 and Jiahe218, and selected homozygous lines with these six genes. We established that photoperiod insensitivity is caused by a defective Hd1 gene. In addition, the effect of Hd1 and Ehd1 on heading date was stronger than that of the other four genes. Measurements of agronomic traits and quality traits in homozygous lines demonstrated the superiority of the hd1 ehd1 genotype for eating quality and photoperiod-insensitive high yield. photoperiodsensitive sensitive longday long day LD (LD shortday short SD (SD change elusive Hd Zhenjing Zhenjing240 analysis Jiahe Jiahe21 SNPs (SNPs floweringrelated related Ehd SDG725 SDG OsCOL15 OsCOL DTH2 DTH DTH7 RILs (RILs addition hd ehd photoperiodinsensitive insensitive yield Zhenjing24 Jiahe2 SDG72 OsCOL1 Zhenjing2 SDG7

ABSTRACT The vertical wall attached jet ventilation has the advantages of good air supply quality, low energy consumption and uniform air distribution, which can improve the environmental quality, reduce heating energy consumption and increase the healthy brood rate when applied to the gosling house in cold region. In order to investigate the distribution of the air flow field and the temperature field in the gosling barn by the computational fluid dynamics method, the three-dimensional steady state simulation of the air flow field and the temperature field is carried out in this study. The results show that the maximum air velocity in the living area of goslings exceeds 0.5m/s when the air supply velocity is greater than 1.8m/s, and goslings feel blowing. When the air supply velocity is lower than 0.8m/s, the average air velocity in the gosling living area is lower than 0.2m/s, and the ventilation is insufficient. When the air supply velocity v=1.5m/s and temperature t=305.15k, the average air velocity in the gosling living area was 0.40m/s, the average temperature was 305.20k, the horizontal sticking range of the air supply air was 7.0m, the Air Diffusion Performance Index of the gosling house was 100%. Ventilation is the best. quality region method threedimensional three dimensional study 05ms ms 0 5m s m 18ms 1 8m 1.8m/s blowing 08ms 0.8m/s 02ms 2m 0.2m/s insufficient v15ms vms v t30515k tk t 305 15k k t=305.15k 040ms 40m 0.40m/s 30520k 20k 305.20k 70m 7 0m 7.0m 100 100% best 30 10 3

RESUMEN Introducción: La mejora continua del nivel de la competición deportiva moderna obliga a la reforma continua y a la actualización constante de la teoría del entrenamiento deportivo, la investigación del entrenamiento deportivo está migrando de la baja intensidad continua a la alta intensidad interválica y muchos estudios han demostrado que el entrenamiento interválico de alta intensidad tiene un buen efecto en los atletas. Objetivo: Investigar el efecto de diferentes modalidades de recuperación durante el entrenamiento de intervalos de alta intensidad en el rendimiento atlético de jóvenes corredores masculinos. Métodos: Mediante la medición del índice morfológico corporal, la prueba de agotamiento intermitente de alta intensidad y el análisis estadístico, se dividió aleatoriamente a cuarenta jóvenes corredores masculinos de larga distancia de escuelas deportivas en el grupo de recuperación activa (AR, n=20) y en el grupo de recuperación pasiva (PR, n=20), se midió el consumo máximo de oxígeno (VO2max) y la velocidad aeróbica máxima (MAV) mediante la prueba de ejercicio de carga incremental, y se registraron la capacidad de ejercicio a intervalos y el tiempo hasta el agotamiento (ET). Resultados: En comparación con el preentrenamiento, MAV y ET en ambos grupos aumentaron significativamente después del entrenamiento (P<0,05); no hubo diferencias significativas en MAV en el grupo de AR después del entrenamiento (P>0,05). Conclusión: El entrenamiento de intervalos de alta intensidad con recuperación activa puede mejorar significativamente la capacidad atlética y el rendimiento de los corredores masculinos jóvenes a largo plazo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción atletas Objetivo Métodos corporal estadístico AR, (AR n=20 n20 n 20 PR, PR (PR n=20, , VO2max VOmax VO max (VO2max (MAV incremental ET. . (ET) Resultados preentrenamiento P<0,05 P005 P 0 05 (P<0,05) P>0,05. P>0,05 (P>0,05) Conclusión plazo II tratamiento n=2 n2 2 (ET P<0,0 P00 (P<0,05 P>0,0 (P>0,05 n= P<0, P0 (P<0,0 P>0, (P>0,0 P<0 (P<0, P>0 (P>0, P< (P<0 P> (P>0 (P< (P> (P

ABSTRACT Introduction: The continuous improvement of the level of modern sports competition compels continuous reform and constant updates on sports training theory; sports training research is migrating from continuous low-intensity to high-intensity interval, and many studies have shown that high-intensity interval training has a good effect on athletes. Objective: Investigate the effect of different recovery modalities during high-intensity interval training on the athletic performance of young male runners. Methods: Using body morphological index measurement, high-intensity intermittent exhaustion test, and statistical analysis, forty young male long-distance runners from sports schools were randomly divided into active recovery group (AR, n=20) and passive recovery group (PR, n=20), maximal oxygen uptake (VO2max) and maximal aerobic speed (MAV) were measured by incremental load exercise test, and interval exercise capacity and time to exhaustion (ET) were recorded. Results: Compared with pre-training, MAV and ET in both groups increased significantly after training (P<0.05); there was no significant difference in MAV on RA group after training (P>0.05). Conclusion: High-intensity interval training with active recovery can significantly improve athletic ability and performance in young male runners in the long term. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction theory lowintensity low intensity highintensity high athletes Objective Methods measurement test analysis longdistance distance AR, AR (AR n=20 n20 n 20 PR, PR (PR n=20, , VO2max VOmax VO max (VO2max (MAV (ET recorded Results pretraining, pretraining pre training, pre-training P<0.05 P005 P 0 05 (P<0.05) P>0.05. P>0.05 . (P>0.05) Conclusion Highintensity High term II outcomes n=2 n2 2 P<0.0 P00 (P<0.05 P>0.0 (P>0.05 n= P<0. P0 (P<0.0 P>0. (P>0.0 P<0 (P<0. P>0 (P>0. P< (P<0 P> (P>0 (P< (P> (P

RESUMO Introdução: O aprimoramento contínuo do nível de competição esportiva moderna compele a reforma contínua e constantes atualizações sobre a teoria do treinamento esportivo, a pesquisa esportiva de treinamento está migrando de contínuo de baixa intensidade para intervalado de alta intensidade e muitos estudos demonstraram que o treinamento intervalado de alta intensidade tem um bom efeito sobre os atletas. Objetivo: Investigar o efeito de diferentes modalidades de recuperação durante o treinamento intervalado de alta intensidade no desempenho atlético de jovens corredores masculinos. Métodos: Usando medição do índice morfológico corporal, teste de exaustão intermitente de alta intensidade e análise estatística, quarenta jovens corredores masculinos de longa distância de escolas esportivas foram divididos aleatoriamente em grupo de recuperação ativa (AR, n=20) e grupo de recuperação passiva (PR, n=20), o consumo máximo de oxigênio (VO2max) e a velocidade aeróbica máxima (MAV) foram medidos pelo teste de exercício de carga incremental, e a capacidade de exercício intervalado e o tempo até a exaustão (ET) foram registrados. Resultados: Em comparação com o pré-treino, MAV e ET nos dois grupos aumentaram significativamente após o treinamento (P<0,05); não houve diferença significativa em MAV no grupo AR após o treinamento (P>0,05). Conclusão: O treinamento em intervalos de alta intensidade com recuperação ativa pode melhorar significativamente a capacidade atlética e o desempenho em corredores jovens masculinos em longo prazo. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução esportivo atletas Objetivo Métodos corporal estatística AR, (AR n=20 n20 n 20 PR, PR (PR n=20, , VO2max VOmax VO max (VO2max (MAV incremental (ET registrados Resultados prétreino, prétreino pré treino, treino pré-treino P<0,05 P005 P 0 05 (P<0,05) P>0,05. P>0,05 . (P>0,05) Conclusão prazo II tratamento n=2 n2 2 P<0,0 P00 (P<0,05 P>0,0 (P>0,05 n= P<0, P0 (P<0,0 P>0, (P>0,0 P<0 (P<0, P>0 (P>0, P< (P<0 P> (P>0 (P< (P> (P

ABSTRACT The selection of key additive materials for a new type of oil-based drilling fluid was studied in this paper. The oil-based drilling fluid can strengthen well walls and ensure safety. It is suitable for high-temperature and high-pressure wells, deep wells and ultra-deep wells. A new type of high-temperature white oil-based drilling fluid was prepared by using 5# white oil as continuous phase, HIS as thickening agent, SDBS as emulsifier, and asphalt powder oxidized at 180°C as filtration agent. The results showed that the saturation concentration of sodium dodecyl benzene sulfonate (SDBS) was 4%, the saturation concentration of asphalt powder oxidized at 180°C was 4%, the saturation concentration of Tween-80 was 10%. The final resistance of high temperature and high pressure oil based drilling fluid formulation material is: No. 5 white oil + 4% SDBS + 5% modified bentonite + 4% asphalt powder oxidized at 180°C + 10% Tween-80 + 1.5% Barite + CaO. The performance evaluation of the new oil-based drilling fluid shows that shale expansion is 0.21mm, anti-temperature up to 220°C, and anti-pollution ability is good. The rheological properties, resistance of high-temperature high- pressure have been greatly improved. Therefore, the new white oil-based drilling fluid can resistance of high- temperature and also has low toxicity, which can meet the exploration and development of more complex wells. oilbased paper safety hightemperature highpressure ultradeep ultra phase agent emulsifier 180C C 180 (SDBS 4 Tween80 Tween 80 Tween-8 10 No 15 1 1.5 CaO 021mm mm 0 21mm 0.21mm antitemperature anti 220C 220 220°C antipollution pollution good properties improved Therefore toxicity 18 Tween8 8 Tween- 1. 22 2

RESUMO Introdução O salto em distância horizontal consiste em quatro partes: corrida de aproximação, impulsão, voo e queda. Objetivo Estudar a influência da ginástica no salto em distância horizontal. Métodos Oito jardins de infância em uma cidade foram selecionados e divididos em um grupo experimental e um grupo de controle. As crianças do grupo experimental atuaram nos módulos de ginástica e de condicionamento físico. Cada módulo foi submetido a um ciclo contínuo de atividades ao ar livre de manhã e à tarde de atividades infantis, de 1º de junho de 2021 a 31 de março de 2010, por um total de 9 meses. Resultados Durante o período de intervenção, não houve diferença significativa entre o grupo experimental e o grupo controle (P > 0,05), mas houve diferença significativa entre o grupo experimental e as meninas antes e depois da intervenção experimental (P < 0,05), enquanto a diferença entre os meninos não foi estatisticamente significativa, indicando que a amplitude do movimento do tornozelo das meninas foi ligeiramente mais afetada do que a dos meninos durante a intervenção de condicionamento físico. Conclusão Com o aumento da idade e do tempo de treinamento, a faixa de variação do ângulo de articulação das crianças mais velhas no grupo experimental foi significativamente maior do que a do grupo de controle. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction The standing long jump consists of four parts: pre-swing, take-off, flying, and landing. Objective Analyze the influence of gymnastics game fitness on standing long jump. Methods Eight kindergartens in a city were selected and divided into experimental and control groups. The children in the experimental group were intervened in gymnastics and game fitness modules. Each module was intervened in a rolling cycle in the morning and afternoon outdoor activities of children from June 1, 2021, to March 31, 2010, for nine months. Results During the intervention period, there was no significant difference between the experimental group and the control group (P > 0.05), but there was a significant difference between the experimental group and the girls before and after the experimental intervention (P < 0.05), while the difference between boys was not statistically significant, indicating that the range of ankle movement of girls was slightly more affected than boys during the fitness intervention. Conclusion With the increase in age and training time, the change range of joint angles of older children in the experimental group is significantly higher than that in the control group. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Introducción El salto de longitud horizontal consta de cuatro partes: carrera de aproximación, impulsión, vuelo y caída. Objetivo Analizar la influencia de la gimnasia en el salto de longitud de pie. Métodos Se seleccionaron ocho guarderías de una ciudad y se dividieron en un grupo experimental y otro de control. Los niños del grupo experimental realizaron módulos de gimnasia y fitness. Cada módulo intervino en un ciclo continuo de actividades al aire libre por la mañana y por la tarde desde el 1 de junio de 2021 hasta el 31 de marzo de 2010, durante nueve meses. Resultados Durante el período de intervención, no hubo diferencias significativas entre el grupo experimental y el grupo de control (P > 0,05), pero sí entre el grupo experimental y las chicas antes y después de la intervención experimental (P < 0,05), mientras que la diferencia entre los chicos no fue estadísticamente significativa, lo que indica que la amplitud de movimiento del tobillo de las chicas se vio ligeramente más afectada que la de los chicos durante la intervención de acondicionamiento físico. Conclusión Al aumentar la edad y el tiempo de entrenamiento, el rango de variación de los ángulos articulares de los niños mayores del grupo experimental es significativamente mayor que el del grupo de control. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: A força conjunta dos membros inferiores desempenha um papel decisivo na capacidade competitiva nos saltadores de salto em distância. O treinamento de força especial baseado na ciência e direcionado para a força das articulações dos membros inferiores é um tópico essencial para os saltadores. Objetivo: Analisar as características de força muscular isocinética das articulações dos membros inferiores em saltadores de salto em distância. Métodos: Saltadores voluntários foram submetidos à testes de contração concêntrica isocinética dos membros inferiores e articulação do quadril. Efetuou-se também a análise do efeito do treinamento de força na lesão das articulações dos membros inferiores. Resultados: Os músculos dos joelhos dos atletas têm uma contratilidade excêntrica reduzida. O tornozelo dos atletas possui a articulação mais vulnerável a lesões no esporte. Conclusão: A força de explosão e a capacidade de contração excêntrica dos músculos extensores dos membros inferiores dos saltadores de salto longo têm o impacto mais significativo no empuxo das articulações e na velocidade de extensão. Os atletas precisam de treinamento de força muscular para desenvolver a força muscular isocinética. Isto pode efetivamente evitar lesões nos movimentos das extremidades inferiores das articulações. Os resultados da pesquisa deste trabalho podem fornecer uma base teórica específica para a formulação do treinamento científico para os saltadores de salto longo. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction: Joint strength of the lower limbs plays a decisive role in the competitive ability of long jumpers. Special strength training based on science and targeted at the strength of the lower limb joints is an essential topic for long jumpers. Objective: To analyze isokinetic muscle strength characteristics of lower limb joints in long jumpers. Methods: Voluntary jumpers were submitted to isokinetic concentric contraction tests of the lower limbs and hip joints. We also analyzed the effect of strength training on lower limb joint injury. Results: The knee muscles of the athletes have reduced eccentric contractility. The ankle of the athlete has the most vulnerable joint to injuries in the sport. Conclusion: The explosive force and eccentric contractility of long jumpers’ lower limb extensor muscles have the most significant impact on joint thrust and extension speed. Athletes need muscle strength training to develop isokinetic muscle strength. This can effectively prevent injury to lower extremity joint movements. The research findings of this paper can provide a specific theoretical basis for formulating scientific training for long jumpers. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: La fuerza articular de los miembros inferiores desempeña un papel decisivo en la capacidad competitiva de los saltadores de longitud. El entrenamiento de fuerza especial basado en la ciencia y dirigido a la fuerza de las articulaciones de los miembros inferiores es un tema esencial para los saltadores. Objetivo: Analizar las características de la fuerza muscular isocinética de las articulaciones de los miembros inferiores en saltadores de longitud. Métodos: Los saltadores voluntarios fueron sometidos a pruebas de contracción concéntrica isocinética de los miembros inferiores y de las articulaciones de la cadera. También se realizó un análisis del efecto del entrenamiento de fuerza en las lesiones de las articulaciones de los miembros inferiores. Resultados: Los músculos de la rodilla de los atletas tienen una contractilidad excéntrica reducida. El tobillo de los atletas tiene la articulación más vulnerable a las lesiones en el deporte. Conclusión: La fuerza de explosión y la contractilidad excéntrica de los músculos extensores de las extremidades inferiores de los saltadores de longitud tienen el impacto más significativo en el empuje articular y la velocidad de extensión. Los atletas necesitan entrenar la fuerza muscular para desarrollar la fuerza muscular isocinética. Esto puede prevenir eficazmente las lesiones en los movimientos de las articulaciones de las extremidades inferiores. Los resultados de la investigación de este trabajo pueden proporcionar una base teórica específica para la formulación del entrenamiento científico de los saltadores de longitud. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Resumo Introdução A manobra de Epley é útil para o retorno da otocônia do braço longo do canal semicircular posterior para o utrículo. Diferentes manobras são necessárias para mover a otocônia para fora do braço curto do canal semicircular posterior e para dentro do utrículo. Objetivo Diagnosticar a VPPB do tipo braço curto do canal semicircular posterior e tratá-la com a manobra de incline and balance. Método Foram diagnosticados 171 casos como VPPB de canal semicircular posterior com base na manobra de Dix-Hallpike positiva. Primeiro tentamos tratar os pacientes com a manobra de incline and balance e, em seguida, executamos a manobra de Dix-Hallpike novamente. Se a repetição da manobra de Dix-Hallpike desse resultados negativos, diagnosticávamos o paciente como VPPB do canal semicircular posterior do tipo braço curto e considerávamos que ele ou ela havia sido curado pela manobra de incline and balance; caso contrário, provavelmente o paciente apresentava VPPB do canal semicircular posterior do tipo braço longo e tratávamos o paciente com a manobra de Epley. Resultados Aproximadamente 40% dos casos foram curados pela manobra de incline and balance, com resultados negativos nas manobras de Dix-Hallpike repetidas, e foram diagnosticados com litíase de braço curto. Conclusão A VPPB de canal semicircular posterior do tipo braço curto pode ser diagnosticada e tratada de maneira conveniente e confortável.

Abstract Introduction The Epley maneuver is useful for the otoconia to return from the long arm of the posterior semicircular canal into the utricle. To move otoconia out of the posterior semicircular canal short arm and into the utricle, we need different maneuvers. Objective To diagnose the short-arm type BPPV of the posterior semicircular canal and treat them with bow-and-yaw maneuver. Methods 171 cases were diagnosed as BPPV of the posterior semicircular canal based on a positive Dix-Hallpike maneuver. We first attempted to treat patients with the bow-and-yaw maneuver and then performed the Dix-Hallpike maneuver again. If the repeated Dix-Hallpike maneuver gave negative results, we diagnosed the patient with the short-arm type of BPPV of the posterior semicircular canal and considered the patient to have been cured by the bow-and-yaw maneuver; otherwise, probably the long-arm type BPPV of the posterior semicircular canal existed and we treated the patient with the Epley maneuver. Results Approximately 40% of the cases were cured by the bow-and-yaw maneuver, giving negative results on repeated Dix-Hallpike maneuvers, and were diagnosed with short-arm lithiasis. Conclusion The short-arm type posterior semicircular canal BPPV can be diagnosed and treated in a convenient and comfortable manner.