Abstract Carbapenem-resistant P. aeruginosa (CRPA) has become a serious public health problem and the biofilm formation aggravates this problem. The study aimed to evaluate the occurrence of β-lactamases and quorum sensing (QS) genes in CRPA isolates, analyze production of biofilm, evaluate the response against meropenem (MPM) and∕or polymyxin B (POL B) and its association with azythromicin (AZT) using quantum dots (QDs) and proteomic analysis. Six CRPA isolates were analyzed. β-lactamases and QS genes were search using specific PCRs and were tested for biofilm production by quantitative technique. A CRPA isolate, containing blaKPC gene and biofilm-producing, was selected to assess its response to therapy using QDs and the MALDI-TOF. The β-lactamase detected was blaKPC in 66.7% of the isolates. All isolates were biofilm producers and carriers of the QS genes. QDs-MPM conjugates triggered the formation of biofilm and the association with AZT inhibited this effect. Proteomics analysis showed that treatments with MPM or POL B suppressed the expression of the transglycosylase protein, while combined therapy with AZT induced expression of the RpoN protein. Thus, this study shows that the use of fluorescence combined with the proteomics analysis was promising to understand how a CRPA strain reacts to antimicrobial treatment.

RESUMO A salinidade é um dos principais problemas agrícolas enfrentados, podendo influenciar negativamente o desenvolvimento vegetal. Porém, estudos têm demonstrado que em ambientes protegidos e sob aplicação de adubos orgânicos, os efeitos deletérios são amenizados. Nesse contexto, objetivou-se avaliar os efeitos da salinidade da água de irrigação sobre a nutrição mineral em plantas de noni, cultivadas em dois ambientes sob adubação com esterco bovino. O delineamento experimental foi em blocos ao acaso, disposto no esquema de parcelas subsubdivididas com cinco repetições. As parcelas foram constituídas pelos ambientes de cultivo (céu aberto e telado), as subparcelas pelas salinidades da água de irrigação (0,3; 1,5; 3,0; 4,5 e 6,0 dS m-1) e as subsubparcelas pela ausência e presença de matéria orgânica, com a unidade experimental consistindo de três vasos, totalizando 300 vasos. Foram determinados as concentrações foliares de nitrogênio, potássio, cálcio, magnésio, sódio e cloreto. Verificou-se que os teores foliares de Ca2+, Mg2+, Na+ e Cl- e a razão Na/K aumentaram com o aumento da salinidade da água de irrigação, sendo os maiores incrementos observados no ambiente a céu aberto. A matéria orgânica não é relevante o suficiente para atenuar os efeitos deletérios da água de irrigação na concentração de nutrientes foliares em plantas de noni. A manutenção de menores valores da razão Na/K no tratamento com matéria orgânica é um fator positivo que pode atenuar os efeitos do estresse salino no metabolismo celular.

ABSTRACT Salinity is one of the main agricultural problems faced, and may negatively influence plant development. However, studies have shown that in protected environments and under the application of organic fertilizers, deleterious effects are mitigated. In this context, the objective of this study was to evaluate the effects of irrigation water salinity on mineral nutrition in noni plants grown in two environments and bovine manure application. The statistical design was a randomized block design arranged in split-split plot scheme, with five repetitions. The plots consisted of the cultivation environments (greenhouse and open field), the subplots formed by salinity levels of irrigation water (ECw: 0.3, 1.5, 3.0, 4.5 and 6.0 dS m-1), and the subsubplots were represented by the absence and presence of organic matter, with experimental unit consisting of three pots totaling 300 pots. The nitrogen, potassium, calcium, magnesium, sodium and chloride concentrations were determined in plant leaves. It was verified that the foliar concentrations of Ca2+, Mg2+, Na+ and Cl- and the ratio Na/K was increased with increasing salinity of the irrigation water, being the largest increments observed in the open field. Organic matter is not much relevant in attenuating the deleterious effects of irrigation water on the concentration of leaf nutrient in noni plants. The maintenance of lower values of Na/K ratio in the treatment with organic matter is a positive factor that can attenuate the effects of salt stress on cell metabolism.

RESUMO Os escorpiões de interesse médico no Brasil pertencem ao gênero Tityus. Dentre eles, Tityus stigmurus é o principal responsável por picadas de escorpião na região Nordeste. Após uma picada, a peçonha de escorpião se distribui rapidamente para os órgãos, atingindo os rins depressa. Entretanto, há poucos trabalhos sobre fisiopatologia renal do envenenamento por escorpiões. Neste trabalho, avaliamos os efeitos da peçonha de T. stigmurus (TsV) sobre parâmetros renais em rins de ratos isolados. Ratos Wistar (n = 6), pesando 250-300 g, foram perfundidos com solução de Krebs-Henseleit contendo 6 g/100 mL de albumina de soro bovino. TsV nas concentrações de 0,3 e 1,0 μg/mL foi testado e os efeitos sobre a pressão de perfusão (PP), resistência vascular renal (RVR), fluxo urinário (FU), taxa de filtração glomerular (TFG) e excreção de eletrólitos foram analisados. Apenas na concentração de 1,0 μg/mL de TsV foram observados efeitos que aumentaram PP (controlePP40' = 92,7 ± 1,95; TsVPP40' = 182,0 ± 4,70 mmHg*, *p < 0,05), RVR (controleRVR40' = 3,28 ± 0,23 mmHg; TstRVR40' = 6,76 ± 0,45 mmHg*, *p < 0,05), FU (controleFU50' = 0,16 ± 0,04; TstFU50' = 0,60 ± 0,10 mL/g/min*, *p < 0,05), a TFG e excreção de eletrólitos, com alterações histológicas que indicam de lesão renal tubular. Em conclusão, peçonha de T. stigmurus induz um aumento transiente da PP com lesão tubular, ambos os quais conduzem a uma excreção elevada de eletrólitos.

ABSTRACT Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurus is the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are few studies concerning the renal pathophysiology of scorpion poisoning. In this study, we evaluated the effects of T. stigmurus venom (TsV) on renal parameters in isolated rat kidneys. Wistar rats (n = 6), weighing 250-300 g, were perfused with Krebs-Henseleit solution containing 6 g/100 mL bovine serum albumin. TsV at 0.3 and 1.0 μg/mL was tested, and the effects on perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR), and electrolyte excretion were analyzed. Effects were observed only at TsV concentration of 1.0 μg/mL, which increased PP (controlPP40' = 92.7 ± 1.95; TsVPP40' = 182.0 ± 4.70* mmHg, *p < 0.05), RVR (controlRVR40' = 3.28 ± 0.23 mmHg; TstRVR40' = 6.76 ± 0.45* mmHg, *p < 0.05), UF (controlUF50' = 0.16 ± 0.04; TstUF50' = 0.60 ± 0.10* mL/g/min,*p < 0.05), GFR and electrolyte excretion, with histological changes that indicate renal tubular injury. In conclusion, T. stigmurus venom induces a transient increase in PP with tubular injury, both of which lead to an augmented electrolyte excretion.