Resumo: “Ritual” é um dos conceitos centrais da cultura confucionista. O duque Zhou desenvolveu a noção de “ritual”, como um conjunto de normas comportamentais, para manter o sistema hierárquico da linhagem ancestral e como regulamentações para governança estatal e práticas cerimoniais. O confucionismo pré-Qin herdou e inovou os rituais Zhou, formando o influente sistema ritual confucionista, profundamente enraizado na sociedade tradicional chinesa. O sistema ritual incorpora elementos de monarquia absoluta, reverência ancestral e piedade filial, refletindo os fatores culturais tradicionais da China. Ele detém funções sociais significativas, servindo como uma ferramenta poderosa para conservar a ordem social, promover a estabilidade e garantir o bem-estar das gerações futuras. Este artigo começa com os três principais aspectos da relação entre benevolência e ritual, governança por meio da virtude e ritual e lei. Ele explica o espírito de benevolência, a ética política amigável às pessoas e a moralidade inerente da lei, contida na ética da etiqueta confucionista. Ademais, analisa o valor moderno da cultura e do espírito da etiqueta confucionista, para a construção moral e legal da sociedade chinesa contemporânea. Resumo Ritual “Ritual confucionista , “ritual” comportamentais cerimoniais préQin pré Qin absoluta filial China significativas social bemestar bem estar futuras lei Ademais contemporânea “ritual

Abstract: “Ritual” is one of the core concepts in Confucian culture. Duke Zhou developed the notion of “ritual” as a set of behavioral norms to maintain the hierarchical system of the ancestral lineage and as regulations for state governance and ceremonial practices. Pre-Qin Confucianism inherited and innovated the Zhou rituals, forming the influential Confucian ritual system deeply embedded in traditional Chinese society. The ritual system embodies elements of absolute monarchy, ancestral reverence and filial piety, reflecting China’s traditional cultural factors. It holds significant social functions, serving as a powerful tool for maintaining social order, promoting stability and ensuring the well-being of future generations. This article starts with the three main aspects of the relationship between benevolence and ritual, governance through virtue, and ritual and law. It explains the spirit of benevolence, people-friendly political ethics and the inherent morality of the law contained in Confucian etiquette ethics, and further analyzes the modern value of Confucian etiquette culture and spirit to the contemporary Chinese society’s moral construction and legal one. Abstract Ritual “Ritual “ritual practices PreQin Pre Qin rituals society monarchy piety Chinas China s factors functions order wellbeing well being generations virtue peoplefriendly people friendly societys

Abstract Objective: Aortic Dissection (AD) is one of the most fatal acute diseases in cardiovascular diseases, with rapid onset and progression and a high fatality rate. This study aims to investigate the clinical values of non-enhancement peripheral pulse-gating rapid magnetic resonance imaging in deterministic diagnosis of AD. Methods: Aorta magnetic resonance imaging was performed in 21 healthy volunteers at a 1.5t MR scanner sequences including cardiac-gated and peripheral pulse-gated True-FISP and HASTE were carried out separately. Acquisition Time (TA), Signal to Noise Ratio (SNR), Contrast Noise Ratio (CNR), and entirety of vessel wall blood flow artifacts were measured and compared. A total of 56 AD cases were displayed by non-enhancement peripheral pulse-gating fast MR imaging, and the results were compared with pathological findings or CTA of the aorta. The dissection rupture, tear film, true and false lumen, thrombosis, hydropericardium, and the main branches of AD were evaluated respectively. Results: There were no significant differences in SNR, CNR, entirety of the vessel wall, and blood flow artifact between cardiac-gated and peripheral pulse-gated fast MR imaging. Non-enhancement pulse-gated fast scanning takes less TA time. By the pulse-gated non-enhancement fast MR imaging, the dissection rupture, tear film, true and false cavity, thrombosis, hydropericardium, and the main branches of aortic dissection were shown clearly. Multi-planar and multi-angle scans helped to show the extent of entrapment rupture, whereas partial complex tears or bi-directional tears were slightly less well visualized. Conclusion: Non-enhancement peripheral pulse-gated rapid magnetic resonance imaging can be used for deterministic diagnosis of AD. Objective (AD rate nonenhancement non enhancement pulsegating pulse gating Methods 2 15t t 1 5t cardiacgated cardiac gated pulsegated TrueFISP True FISP separately TA, , (TA) SNR (SNR) CNR (CNR) 5 aorta rupture film lumen thrombosis hydropericardium respectively Results Nonenhancement Non time cavity clearly Multiplanar Multi planar multiangle multi angle bidirectional bi directional visualized Conclusion (TA (SNR (CNR

Abstract In herbal markets, an overwhelming majority the Dendrobium nobile are sold in dried strips form, and this product type would lead to the incomplete release of the active ingredient. To increase the bioaccessibility of Dendrobium nobile, we obtained powders of different particle sizes by shear-breaking and superfine-grinding to further compare its sensory properties, physical properties, the leaching rate of active ingredients and antioxidant activity in vitro. The results showed that the superfine-grinding changed the sensory properties and physical properties of Dendrobium nobile, with an increase in bulk density, a decrease in swelling capacity and water-holding capacity and an increase in water solubility index. The method also contributed to the release of total polysaccharides, dendrobine, total polyphenols and total flavonoids components, thereby increasing ABTS, DPPH and nitrite ion scavenging activity. In addition, scanning electron microscopy and infrared spectroscopy analyses showed that superfine-grinding had no effect on the primary structure of the fractions as with decreasing particle size. This study suggested that superfine-grinding could be a promising method for producing Dendrobium nobile powders with higher contents of bioactive compounds and bioactivity. markets form ingredient shearbreaking shear breaking superfinegrinding superfine grinding vitro density waterholding holding index polysaccharides dendrobine components ABTS addition size bioactivity

Abstract We aimed to compare the effects of oxycodone hydrochloride and dezocine on hemodynamics and inflammatory factors in patients receiving gynecological laparoscopic surgery under general anesthesia. A total of 246 patients were divided into group A and B (n=123). Hemorheology indices were recorded 5 min after anesthesia (T0), 1 min after pneumoperitoneum (T1), when position was changed 5 min after pneumoperitoneum (T2), 15 min after pneumoperitoneum (T3), 1 min (T4) and 5 min (T5) after position was restored. Visual analogue scale scores 1, 2, 6, 12, 24 and 48 h after operation were recorded. Postoperative adverse reactions and visceral pain were observed. The expression levels of inflammatory factors were detected by enzyme-linked immunosorbent assay 12 h after operation. Compared with group A, group B had higher heart rate and mean arterial pressure at T2, lower central venous pressure and cardiac output at T1-T3, and higher systemic vascular resistance at T1-T5 (P<0.05). The incidence rate of pain syndrome in group A was lower (P<0.05). Group A had lower tumor necrosis factor-alpha and interleukin-6 expression levels and higher interleukin-10 level than those of group B (P<0.05). For gynecological laparoscopic surgery, oxycodone preemptive analgesia has superior outcomes to those of dezocine. n=123. n123 n n=123 . 123 (n=123) T0, T0 T , (T0) T1, T1 (T1) T2 (T2) T3, T3 (T3) T4 (T4 T5 (T5 restored 2 6 4 observed enzymelinked enzyme linked T1T3, T1T3 TT T1-T3 T1T5 T1-T P<0.05. P005 P P<0.05 0 05 (P<0.05) factoralpha factor alpha interleukin6 interleukin interleukin- interleukin10 10 interleukin-1 n12 n=12 (n=123 (T0 (T1 (T2 (T3 (T T1T P00 P<0.0 (P<0.05 interleukin1 n1 n=1 (n=12 P0 P<0. (P<0.0 n= (n=1 P<0 (P<0. (n= P< (P<0 (n (P< (P

Abstract Objective This study aims to perform a meta-analysis to figure out the efficacy of OK-432 sclerotherapy between Macrocystic (MAC) lymphangiomas and Microcystic (MIC) lymphangiomas. Methods We conducted a systematic review and meta-analysis to clarify the relationship between OK-432 and lymphangiomas. PubMed and ISI Web of Science were searched from inception to May 2022. Joanna Briggs Institute (JBI) manual was used to evaluate the risk of bias. We calculated pooled Relative Risks (RR) and 95% Confidence Interval (95% CI) using random effects model to evaluate the relations between OK-432 and lymphangiomas. Results A total of 11 studies (including 352 cases) about OK-432 sclerotherapy for lymphangioma were included in the current meta-analyses. The results suggested that the efficacy of OK-432 was significantly in MAC lesions than in MIC (RR = 1.51, 95% CI 1.298-1.764), with significant moderate degrees of heterogeneity among 11 studies (I2 = 51.2%, p = 0.025). Subgroup analyses suggested that there was significant association in both retrospective studies (RR = 1.26, 95% CI 1.03-1.53) and classification (by 1 cm) (RR = 1.37, 95% CI 1.04-1.80) were associated with the efficacy of OK-432. Conclusion To our knowledge, our study represents the first meta-analysis examining the efficacy of OK-432 in the treatment of different types of LMs. However, the regional differences and the age differences of the subjects are the main limitations of this study and should be avoided in further research. Our results suggested that OK-432 sclerotherapy for macrocystic lymphangiomas was more effective. metaanalysis meta analysis OK432 OK 432 OK-43 (MAC (MIC 2022 JBI (JBI bias RR 95 (95 including 35 cases metaanalyses. metaanalyses analyses. meta-analyses 151 51 1.51 1.2981.764, 12981764 1.298 1.764 , 298 764 1.298-1.764) I2 I (I 512 2 51.2% 0.025. 0025 0.025 . 0 025 0.025) 126 26 1.26 1.031.53 103153 1.03 1.53 03 53 1.03-1.53 by cm 137 37 1.37 1.041.80 104180 1.04 1.80 04 80 1.04-1.80 OK432. 432. knowledge LMs However research effective OK43 43 OK-4 202 9 (9 3 15 5 1.5 2981 1.2981.764 1298176 1298 1.29 1764 1.76 29 76 1.298-1.764 51.2 002 0.02 02 12 1.2 031 1.031.5 10315 103 1.0 153 1.03-1.5 13 1.3 041 1.041.8 10418 104 180 1.8 8 1.04-1.8 OK4 4 OK- 20 ( 1. 1.2981.76 129817 129 176 1.7 7 1.298-1.76 51. 00 0.0 1.031. 1031 10 1.03-1. 1.041. 1041 18 1.04-1. 1.2981.7 12981 17 1.298-1.7 0. 1.031 1.03-1 1.041 1.04-1 1.2981. 1.298-1. 1.03- 1.04- 1.2981 1.298-1 1.298-

ABSTRACT Purpose: To investigate the role of cyanidin-3-O-glucoside (C3G) in renal ischemia/reperfusion (I/R) injury and the potential mechanisms. Methods: Mouse models were established by clamping the left renal vessels, and in vitro cellular models were established by hypoxic reoxygenation. Results: Renal dysfunction and tissue structural damage were significantly higher in the I/R group. After treatment with different concentrations of C3G, the levels of renal dysfunction and tissue structural damage decreased at different levels. And its protective effect was most pronounced at 200 mg/kg. The use of C3G reduced apoptosis as well as the expression of endoplasmic reticulum stress (ERS)-related proteins. Hypoxia/reoxygenation (H/R)-induced apoptosis and ERS are dependent on oxidative stress in vitro. In addition, both AG490 and C3G inhibited the activation of JAK/STAT pathway and attenuated oxidative stress, ischemia-induced apoptosis and ERS. Conclusions: The results demonstrated that C3G blocked renal apoptosis and ERS protein expression by preventing reactive oxygen species (ROS) production after I/R via the JAK/STAT pathway, suggesting that C3G may be a potential therapeutic agent for renal I/R injury. Purpose cyanidin3Oglucoside cyanidinOglucoside cyanidin 3 O glucoside CG C G (C3G ischemiareperfusion ischemia reperfusion IR I R (I/R mechanisms Methods vessels reoxygenation Results group 20 mgkg mg kg mg/kg ERSrelated related proteins Hypoxiareoxygenation Hypoxia H/Rinduced HRinduced H/R induced H addition AG AG49 JAKSTAT JAK STAT ischemiainduced Conclusions ROS (ROS Oglucoside 2 Rinduced HR AG4

RESUMEN Introducción: La citoquina eritropoyetina (EPO), se conoce como una hormona crucial para la producción de glóbulos rojos, que transportan la sangre oxigenada al resto del cuerpo. Objetivos: El objetivo de este trabajo fue crear una detección electroquímica basada en nanopartículas de Fe2O3-NiO y óxido de grafeno para medir los niveles de EPO en la sangre de los deportistas. Métodos: Sobre un electrodo de carbono vítreo, se sintetizó Fe2O3-NiO@GO mediante el método de deposición electroquímica. Resultados: El Fe2O3-NiO@GO/GCE fue validado por caracterizaciones estructurales mediante microscopía electrónica de barrido (SEM). El Fe2O3-NiO@GO/GCE resultó ser un biosensor de eritropoyetina adecuado y estable con un rango lineal de 0-500 ng/l y un límite de detección de 0,03ng/l en ensayos electroquímicos mediante la técnica DPV. Se investigó el uso de Fe2O3-NiO@GO/extropoietina como biosensor de eritropoyetina en el plasma de atletas. Conclusión: Los resultados mostraron que los valores obtenidos para la recuperación (94,56% a 98,40) y la RSD (2,01% a 3,22%) fueron aceptables, lo que indica que la técnica sugerida puede ser utilizada como un biosensor práctico de eritropoyetina en muestras de sangre. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción EPO, , (EPO) rojos cuerpo Objetivos Fe2O3NiO FeONiO Fe2O3 NiO Fe O deportistas Métodos vítreo Fe2O3NiO@GO Fe2O3NiOGO FeONiOGO NiO@GO GO Resultados Fe2O3NiO@GO/GCE Fe2O3NiOGOGCE FeONiOGOGCE NiO@GO/GCE GCE SEM. SEM . (SEM) 0500 0 500 0-50 ngl ng l 003ngl 03ng DPV Fe2O3NiO@GO/extropoietina Fe2O3NiOGOextropoietina FeONiOGOextropoietina NiO@GO/extropoietina extropoietina atletas Conclusión 94,56% 9456 94 56 (94,56 98,40 9840 98 40 2,01% 201 2 01 (2,01 3,22% 322 3 22 aceptables II tratamiento (EPO FeO Fe2O NiOGO NiOGOGCE (SEM 050 50 0-5 NiOGOextropoietina 94,56 945 9 5 (94,5 98,4 984 4 2,01 20 (2,0 3,22 32 05 0- 94,5 (94, 98, 2,0 (2, 3,2 94, (94 2, (2 3, (9 (

RESUMO Introdução: A citocina eritropoietina (EPO), é referida como um hormônio crucial para a produção de hemácias, que transportam o sangue oxigenado para o resto dos corpos. Objetivos: O objetivo deste trabalho foi criar uma detecção eletroquímica baseada em nanopartículas de Fe2O3-NiO e óxido de grafeno para medir os níveis de EPO no sangue dos atletas. Métodos: Em um eletrodo de carbono vítreo, o Fe2O3-NiO@GO foi sintetizado usando o método de deposição eletroquímica. Resultados: O Fe2O3-NiO@GO/GCE foi validado por caracterizações estruturais utilizando microscopia eletrônica de varredura (SEM). O Fe2O3-NiO@GO/GCE foi considerado um biosensor eritropoietina adequado e estável com uma faixa linear de 0-500 ng/l e um limite de detecção de 0,03ng/l em testes eletroquímicos utilizando a técnica DPV. O Fe2O3-NiO@GO/extropoietina foi investigado como um biosensor de eritropoietina no plasma do atleta. Conclusão: Os resultados mostraram que os valores obtidos para recuperação (94,56% a 98,40) e RSD (2,01% a 3,22%) foram aceitáveis, indicando que a técnica sugerida pode ser usada como um prático biosensor de eritropoietina em amostras de sangue. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução EPO, , (EPO) hemácias corpos Objetivos Fe2O3NiO FeONiO Fe2O3 NiO Fe atletas Métodos vítreo Fe2O3NiO@GO Fe2O3NiOGO FeONiOGO NiO@GO GO Resultados Fe2O3NiO@GO/GCE Fe2O3NiOGOGCE FeONiOGOGCE NiO@GO/GCE GCE SEM. SEM . (SEM) 0500 0 500 0-50 ngl ng l 003ngl 03ng DPV Fe2O3NiO@GO/extropoietina Fe2O3NiOGOextropoietina FeONiOGOextropoietina NiO@GO/extropoietina extropoietina atleta Conclusão 94,56% 9456 94 56 (94,56 98,40 9840 98 40 2,01% 201 2 01 (2,01 3,22% 322 3 22 aceitáveis II tratamento (EPO FeO Fe2O NiOGO NiOGOGCE (SEM 050 50 0-5 NiOGOextropoietina 94,56 945 9 5 (94,5 98,4 984 4 2,01 20 (2,0 3,22 32 05 0- 94,5 (94, 98, 2,0 (2, 3,2 94, (94 2, (2 3, (9 (

ABSTRACT Introduction: The cytokine erythropoietin (EPO) is a crucial hormone for producing RBCs, which carry oxygenated blood to the rest of the body. Objective: This paper aimed to create an electrochemical detection based on Fe2O3-NiO nanoparticles and graphene oxide to measure EPO levels in athletes’ blood. Methods: On a glassy carbon electrode, Fe2O3-NiO@GO was synthesized using the electrochemical deposition method. Results: The Fe2O3-NiO@GO/GCE was validated by structural characterizations using scanning electron microscopy (SEM). The Fe2O3-NiO@GO/GCE was found to be a suitable and stable erythropoietin biosensor with a linear range of 0-500 ng/l and a detection limit of 0.03ng/l in electrochemical tests using the DPV technique. Fe2O3-NiO@GO/erythropoietin was investigated as a biosensor for erythropoietin in athlete's plasma. Conclusion: The results showed that the values obtained for recovery (94.56% to 98.40) and RSD (2.01% to 3.22%) were acceptable, indicating that the suggested technique can be used as a practical erythropoietin biosensor in blood samples. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction (EPO RBCs body Objective Fe2O3NiO FeONiO Fe2O3 NiO Fe O athletes Methods electrode Fe2O3NiO@GO Fe2O3NiOGO FeONiOGO NiO@GO GO method Results Fe2O3NiO@GO/GCE Fe2O3NiOGOGCE FeONiOGOGCE NiO@GO/GCE GCE SEM. SEM . (SEM) 0500 0 500 0-50 ngl ng l 003ngl 03ng Fe2O3NiO@GO/erythropoietin Fe2O3NiOGOerythropoietin FeONiOGOerythropoietin NiO@GO/erythropoietin athlete s plasma Conclusion 94.56% 9456 94 56 (94.56 98.40 9840 98 40 2.01% 201 2 01 (2.01 3.22% 322 3 22 acceptable samples II outcomes FeO Fe2O NiOGO NiOGOGCE (SEM 050 50 0-5 NiOGOerythropoietin 94.56 945 9 5 (94.5 98.4 984 4 2.01 20 (2.0 3.22 32 05 0- 94.5 (94. 98. 2.0 (2. 3.2 94. (94 2. (2 3. (9 (

RESUMEN Introducción: En el deporte de orientación, el practicante tiene que pasar por puntos de control marcados en el terreno en el menor tiempo posible, con la ayuda de un mapa y una brújula, utilizando la propia naturaleza como campo de juego. Esta actividad acompaña a la humanidad desde su origen y, como deporte, apareció en los países nórdicos hace más de cien años, con el propósito de realizar una actividad física e intelectual al aire libre, con el potencial de promover la resistencia aeróbica de los estudiantes universitarios. Objetivo: Estudiar el efecto del deporte de orientación en la resistencia física de los estudiantes universitarios. Métodos: Mediante el método de investigación experimental, se tomaron como objeto de investigación 30 estudiantes universitarios de diferentes carreras, se midieron los índices fisiológicos de cada grupo y se analizó el efecto del deporte sobre la función cardiopulmonar y la resistencia aeróbica de los estudiantes universitarios y su mecanismo fisiológico. Resultados: Los estudiantes universitarios de orientación que han realizado un entrenamiento deportivo durante mucho tiempo y las asignaturas de educación física tienen una función cardiopulmonar y una resistencia aeróbica significativamente mejores que los estudiantes universitarios ordinarios en diversos grados. Conclusión: El deporte de orientación demostró un efecto positivo en la resistencia física de los estudiantes, aumentando sus índices de resistencia cardiopulmonar y aeróbica. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción posible brújula juego años libre Objetivo Métodos experimental 3 carreras fisiológico Resultados grados Conclusión II tratamiento

RESUMO Introdução: No esporte de orientação, o praticante tem que passar por pontos de controle marcados no terreno no menor tempo possível, com o auxílio de um mapa e de uma bússola, usando a própria natureza como campo de jogo. Essa atividade acompanha o homem desde a sua origem e, como esporte, surgiu nos países nórdicos há mais de cem anos, com o propósito de realizar-se uma atividade física e intelectual ao ar livre, com o potencial de promover os a resistência aeróbica dos estudantes universitários. Objetivo: Estudar o efeito do esporte de orientação sobre a resistência física dos estudantes universitários. Métodos: Através do método de pesquisa experimental, 30 estudantes universitários de diferentes áreas de habilitação foram tomados como objetos de pesquisa, os índices fisiológicos de cada grupo foram medidos e o efeito do esporte sobre a função cardiopulmonar e resistência aeróbica dos estudantes universitários e seu mecanismo fisiológico foram discutidos. Resultados: Os estudantes universitários de orientação que se dedicam ao treinamento esportivo há muito tempo e as disciplinas de educação física têm função cardiopulmonar e resistência aeróbica significativamente melhores do que os estudantes universitários comuns em graus variados. Conclusão: O esporte de orientação demonstrou um efeito positivo sobre a resistência física dos estudantes, aumentando seus índices cardiopulmonares e de resistência aeróbica. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução possível bússola jogo anos realizarse realizar livre Objetivo Métodos experimental 3 discutidos Resultados variados Conclusão II tratamento

ABSTRACT Introduction: In the orienteering sport, the orienteer has to pass through control points marked on the terrain in the shortest possible time, with the help of a map and a compass, using nature as a playing field. This activity has been with man since its origin. As a sport, it appeared in the Nordic countries more than a hundred years ago to perform an outdoor physical and intellectual activity with the potential to promote the aerobic endurance of university students. Objective: Study the effect of orienteering sports on the physical endurance of college students. Methods: Through experimental research method, 30 college students from different majors were taken as research objects, the physiological indices of each group were measured, and the effect of sport on cardiopulmonary function and aerobic endurance of college students and its physiological mechanism were discussed. Results: Orientation college students who have been engaged in sports training for a long time and physical education subjects have a significantly better cardiopulmonary function and aerobic endurance than ordinary college students to varying degrees. Conclusion: Orientation sports positively affected students’ physical endurance, increasing their cardiopulmonary and aerobic endurance indexes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction compass field origin Objective Methods method 3 objects measured discussed Results degrees Conclusion indexes II outcomes

RESUMEN La contaminación por neblina, caracterizada principalmente por la baja visibilidad, es uno de los principales problemas ambientales a los que se enfrenta China en la actualidad. El pronóstico exacto de la neblina es útil para la aplicación de medidas preventivas orientadas a controlar emisiones de contaminantes atmosféricos y aliviar la contaminación por neblina. Sin embargo, no es fácil predecir con precisión los acontecimientos de baja visibilidad causados por neblina, lo cual requiere no sólo un pronóstico riguroso de los factores meteorológicos, sino también una actualización detallada y en tiempo real de los inventarios de emisiones de las fuentes. Con el fin de obtener una herramienta de predicción fiable, se estudian varios métodos populares de aprendizaje automático, como la máquina vectorial de apoyo (SVM, por su sigla en inglés), el k-vecino más próximo, el bosque aleatorio y varios métodos de aprendizaje profundo. A partir de los principales factores que influyen en la visibilidad, se analizan las relaciones entre velocidad y dirección del viento, temperatura, humedad y visibilidad. El método de aprendizaje automático se utiliza para entrenamiento y pronóstico. La precisión de estos métodos para pronosticar la visibilidad se verifica mediante varios parámetros (error de raíz cuadrada media, error absoluto medio y error porcentual absoluto medio). Los resultados muestran que: 1) entre todos los parámetros meteorológicos, la velocidad del viento puede reflejar mejor la variación de la visibilidad; 2) el rendimiento de las redes neuronales recurrentes de memoria a largo-corto plazo (LSTM RNN) y el método de unidades recurrentes cerradas (GRU) en la predicción de visibilidad a corto plazo (es decir, 1, 3 y 6 h) es casi igual; 3) el método clásico de aprendizaje automático (SVM) funciona bien para el pronóstico de visibilidad a medio y largo plazo, y 4) el método de aprendizaje automático también tiene cierto grado de precisión en el pronóstico, incluso en un periodo de tiempo más largo (p. ej., 72 h).

ABSTRACT Haze pollution, mainly characterized by low visibility, is one of the main environmental problems currently faced by China. Accurate haze forecasts facilitate the implementation of preventive measures to control the emission of air pollutants and thereby mitigate haze pollution. However, it is not easy to accurately predict low visibility events induced by haze, which requires not only accurate prediction for weather elements, but also refined and real-time updated source emission inventory. In order to obtain reliable forecasting tools, this paper studies the usability of several popular machine learning methods, such as support vector machine (SVM), k-nearest neighbor, and random forest, as well as several deep learning methods, on visibility forecasting. Starting from the main factors related to visibility, the relationships between wind speed, wind direction, temperature, humidity, and visibility are discussed. Training and forecasting were performed using the machine learning methods. The accuracy of these methods in visibility forecasting was confirmed through several parameters (i.e., root-mean-square error, mean absolute error, and mean absolute percentage error). The results show that: (1) among all meteorological parameters, wind speed was the best at reflecting the visibility change patterns; (2) long short-term memory recurrent neural networks (LSTM RNN), and gated recurrent unit (GRU) methods perform almost equally well on short-term visibility forecasts (i.e., 1, 3, and 6 h); (3) a classical machine learning method (i.e., the SVM) performs well in mid- and long-term visibility forecasts; (4) machine learning methods also have a certain degree of forecast accuracy even for long time periods (e.g., 7 2h).

Abstract Background: UVB irradiation can cause acute damage such as sunburn, or photoaging and melanoma, all of which are major health threats. Objective: This study was designed to investigate the mechanism of skin photoaging induced by UVB radiation in mice through the analysis of the differential expression of miRNAs. Methods: A UVB irradiation photoaging model was constructed. HE and Masson special stains were used to examine the modifications in the epidermis and dermis of mice. The miRNA expression profiles of the mouse skin model exposed to UVB radiation and the normal skin of mice were analyzed using miRNA-sequence analysis. GO and Pathway analysis were employed for the prediction of miRNA targets. Results: A total of 23 miRNAs were evaluated for significantly different expressions in comparison to normal skin. Among them, 7 miRNAs were up-regulated and 16 were down-regulated in the skin with photoaging of mice exposed to UVB irradiation. The differential expression of miRNA is related to a variety of signal transduction pathways, among which mmu-miR-195a-5p and mitogen-activated protein kinase (MAPK) signal pathways are crucial. There was a significant differential expression of miRNA in the skin of normal mice in comparison with the skin with photoaging induced by UVB irradiation. Study limitations: Due to time and energy constraints, the specific protein level verification, MAPK pathway exploration, and miR-195a-5p downstream molecular mechanism need to be further studied in the future. Conclusions: UVB-induced skin photoaging can be diagnosed and treated using miRNA.

Abstract Background and objectives: Nerve block or neurolysis is an important approach in the treatment of spastic equinovarus foot. To illustrate the accurate location of the nerve branch to the tibialis posterior muscle (TP) in clinical practice, 21 adult cadavers were dissected and 14 complete both lower limb specimens were obtained. A total of 28 lower limbs were included. Methods: We measured the length of the motor branch nerve (LM) of the tibialis posterior muscle, the length of the fibula (LF), the vertical distance (D1) from the midpoint of LM to the fibula tip as well as the horizontal distance (D2) from the midpoint of LM to the inner edge of the fibula. Results: The LM was higher (35.74 ± 7.28 mm) in male than in female (30.40 ± 6.88 mm) specimens but there was no significant correlation between LM and gender (> 0.05). Additionally, among male specimens, the LM on the right side was longer than that on the left (p ≤ 0.05) while among female specimens, the D1 on the left side was longer than that on the right (p ≤ 0.05). The LF in male specimen was significantly longer than that in female (p ≤ 0.05). The midpoint of the nerve to the motor branch of the tibialis posterior muscle was about 50 mm distal to the fibular head and 10 mm at the inner edge of the fibula. Conclusion: Using this coordinate, the midpoint of the nerve branch to the TP could be accurately located.

Abstract To study the effects of prolactin (PRL) in pituitary on the reproduction of the Striped Hamsters via the photoperiods. Mature female striped hamsters were raised under various photoperiods to study the differential expression pattern of PRL in pituitary, and prolactin receptor (PRLR) in the hypothalamus, pituitary and ovary. Photoperiods affect the pituitary PRL mRNA level and pituitary, hypothalamus and ovaries PRLR mRNA levels in striped hamsters. Positive correlations of PRL mRNA expression in the pituitary with that of PRLR in hypothalamus and ovaries, and that of GnRH in hypothalamus were determined. The serum concentration of PRL, FSH, LH and GnRH were significantly different among the striped hamsters from different photoperiods. Serum concentration of PRL is positively correlated with that of FSH, LH and GnRH of the striped hamsters. Photoperiod was important environment factor modulating the expression profile of PRL and PRLR in the striped hamsters.

Abstract Transfection efficiency was estimated to optimize the conditions for RNA interference (RNAi), including transfection time, validity, and nucleic acid concentration and type, using the EZ Trans Cell Reagent, a cationic polymer. An shRNA against GFP was designed and transfected into cells using the EZ transfection reagent. The shRNA significantly decreased the expression of GFP. In addition, pre-diluted transfection reagent at room temperature and small nucleic acids increased the transfection efficiency, which peaked at 24 h. Compared with circular nucleic acids, linear nucleic acids showed higher transfection efficiency and a higher genome integration rate. We optimized cationic polymer-mediated RNAi conditions, and our data will be useful for future RNAi studies.

ABSTRACT Purpose: Increased attention has been focused on the survival of renal cell carcinoma (RCC) patients with bone metastasis. This study proposed to establish and evaluate a nomogram for predicting the overall survival (OS) and cancer-specific survival (CSS) of RCC patients with bone metastasis. Materials and Methods: RCC patients with bone metastasis between 2010 and 2015 were captured from the surveillance, epidemiology and end results (SEER) database. Univariate and multivariate cox regressions were performed to assess the effects of clinical variables on OS and CSS. The nomogram based on the Cox hazards regression model was developed. Concordance index (C-index) and calibration curve were performed to evaluate the accuracy of nomogram models, receiver operating characteristic (ROC) curves and decision curve analysis (DCA) were conducted to assess the predict performance. Results: A total of 2.471 eligible patients were enrolled in this study. The patients were assigned to primary (n=1.672) and validation (n=799) cohorts randomly. The 1-, 2-, and 3-year OS and CSS nomogram models were constructed based on age at diagnosis, sex, marital status, pathological grade, T-stage, N-stage, brain/liver/lung metastasis, surgery, radiotherapy and chemotherapy. The c for OS and CSS prediction was 0.730 (95% confidence interval [CI]: 0.719-0.741) and 0.714 (95%CI:0.702-0.726). The calibration curves showed significant agreement between nomogram models and actual observations. ROC and DCA indicated nomograms had better predict performance. Conclusions: The nomograms for predicting prognosis provided an accurate prediction of OS and CSS in RCC patients with bone metastasis, and contributed clinicians to optimize individualized treatment plans.