RESUMO Objetivo Em 2012, uma nova regulamentação brasileira proibiu o uso de aditivos aromáticos nos produtos tabagísticos. Para entender melhor o impacto potencial desta regulação, este estudo examinou como a propaganda de aroma em embalagens de cigarros influencia a percepção das brasileiras jovens sobre a marca do produto. Método Estudo transversal em que 640 brasileiras (168 fumantes e 458 não fumantes) avaliaram online, uma entre três das seguintes características de 10 pacotes de cigarros: 1) embalagem com a imagem da marca; 2) pacotes com o mesmo tamanho, formato e descrição das características do produto 1, mas sem marca (havia apenas a descrição do produto) e, 3) embalagens nas condições 2, mas sem a descrição do produto (embalagem em branco). O modelo de regressão linear misto foi utilizado para determinar associações das características da embalagem do cigarro (Ex: situação experimental, descrição de aroma versus não, pacotes finos versus não) com as avaliações das participantes para nove características do produto, incluindo a apelação, aroma, suavidade e atributos de quem fuma certa marca. Resultados Pacotes aromatizados de marca foram classificados como mais atraentes, com melhor sabor e suavidade em relação aos que tinham simples descrição do produto. Os pacotes descritos como aromatizados comparados ao mesmo produto sem descrição obtiveram menores escores para oito dentre nove características avaliadas. Em comparação as não fumantes sem suscetibilidade ao tabagismo, as que eram suscetíveis classificaram produtos aromatizados mais positivamente em oito de suas nove características. As embalagens finas receberam julgamento mais positivo do que as com espessura regular para oito dentre nove itens analisados. Conclusões Pacotes finos e marcas que destacam os aromas do tabaco parecem aumentar a percepção positiva deste produto. Proibir a aromatização do tabaco e a oferta de pacotes finos de cigarros reduzem o apelo de fumar entre brasileiras jovens, bem como para outros grupos vulneráveis.

ABSTRACT Objective In 2012, a new Brazilian regulation prohibited the use of flavor additives in tobacco products. To better understand the potential impact of this regulation, this study examines how flavor descriptors on cigarette packaging influence brand perceptions among young Brazilian women. Methods An online cross-sectional experiment was conducted with Brazilian women aged 16–26 (N = 640: 182 smokers and 458 nonsmokers) who rated 10 cigarette packages from one of three conditions: 1) branded packs; 2) packs with the same size, shape, and verbal descriptions as in condition 1, but without brand imagery (i.e., “plain pack”); and 3) packs from condition 2 but without brand descriptors (i.e., “plain pack, no descriptors”). Mixed-effects linear regression models were utilized to determine what associations that pack features (i.e., experimental condition; flavor descriptor vs. not; slim pack vs. not) had with participant ratings of nine characteristics, including appeal, taste, smoothness, and attributes of people who smoke the brand. Results Flavored branded packs were rated as more appealing, better tasting, and smoother than flavored plain packs with descriptors. Compared to flavored plain packs with descriptors, the same packs without descriptors were rated less positively on eight of the nine characteristics. Compared to nonsusceptible nonsmokers, susceptible nonsmokers rated flavored packs more positively on eight of the nine characteristics. Slim packs were rated more positively than regular packs on eight of the nine characteristics. Conclusions Slim packs and brands highlighting tobacco flavors appear to increase positive perceptions of tobacco products. Banning tobacco flavorings and slim packs may reduce the appeal of smoking for young Brazilian women, as well as for other vulnerable populations.

RESUMEN Objetivo En 2012, una nueva regulación brasileña prohibió el uso de aditivos de sabor en los productos de tabaco. Para comprender mejor el impacto potencial de esta regulación, este estudio examina cómo los descriptores de sabor en los envases de cigarrillos influyen en las percepciones de marca entre las jóvenes brasileñas. Métodos Se realizó un estudio transversal, en línea con mujeres brasileñas de entre 16 y 26 años (N = 640: 182 fumadores y 458 no fumadores) que calificaron 10 paquetes de cigarrillos de acuerdo a una de las siguientes condiciones: 1) paquetes de marca; 2) paquetes con el mismo tamaño, forma y descripciones verbales que en la condición 1, pero sin imágenes de marca (es decir, “paquete simple”); y 3) paquetes de la condición 2 pero sin descriptores de marca (es decir, “paquete simple, sin descriptores”). Se utilizaron modelos de regresión lineal de efectos mixtos para determinar qué asociación existía entre las diferentes condiciones (es decir, condición experimental, descriptor de sabor vs. no, paquete delgado vs. no) con las calificaciones de los participantes de nueve características, incluyendo atractivo, sabor, suavidad y atributos de personas que fuman la marca. Resultados Los paquetes de marca con sabor se calificaron como más atractivos, de mejor sabor y más suaves que los paquetes simples con sabores y descriptores. En comparación con los paquetes simples con sabores con descriptores, los mismos paquetes sin descriptores fueron calificados de manera menos positiva en ocho de las nueve características. En comparación con los no fumadores no susceptibles, los no fumadores susceptibles calificaron los paquetes con sabor de forma más positiva en ocho de las nueve características. Los paquetes delgados fueron clasificados más positivamente que los paquetes regulares en ocho de las nueve características. Conclusiones Los paquetes delgados y las marcas que destacan los sabores del tabaco parecen aumentar las percepciones positivas de los productos de tabaco. Prohibir los sabores del tabaco y los paquetes delgados puede reducir el atractivo del tabaco para las jóvenes brasileñas, así como para otras poblaciones vulnerables.

Both domestic and wild carnivore species are commonly diagnosed with rabies virus (RABV) infection in South Africa. Although the majority of confirmed rabies cases in wild carnivore species are reported from the yellow mongoose (Cynictis penicillata), the rest are from other wild carnivores including the highly endangered wild dog (Lycaon pictus). Lyssavirus infection was confirmed in two wild dogs and a spotted hyaena (Crocuta crocuta) in the Madikwe Game Reserve, North West province in South Africa, in 2014 and 2015, using a direct fluorescent antibody test and immunohistochemistry. There had been no new wild dog introductions to the Madikwe Game Reserve for many years and the wild dogs were last vaccinated against rabies approximately 11 years prior to the incident. The first euthanised wild dog was the last surviving of a break-away pack of 6, and the second was the last of a larger pack of 18, the rest of which died with no carcasses being found or carcasses too decomposed for sampling. Subsequent antigenic typing of the lyssaviruses indicated that they were canid RABVs. The RABVs originating from 22 wild carnivore species, 7 dogs, and a caprine, mostly from the North West province, were genetically characterised by targeting a partial region of the nucleoprotein gene. The nucleotide sequence analyses of these viruses and two previously characterised RABVs confirmed that the outbreak viruses were also canid rabies, phylogenetically clustering with virus isolates originating from black-backed jackals recovered between 2012 and 2015 from the North West province, and domestic dogs from neighbouring communal areas. The source(s) of the mortalities and possible reservoir host(s) for the virus could only be speculated upon from data on specific predator numbers, movements and behaviour, kills, park management and the changing environmental ecology, which were monitored closely in Madikwe over several years. The most likely rabies sources were from boundary fence contacts between wild carnivores within the park, with domestic dogs or cats and/or naturally occurring wild carnivores outside the park. The associated risk of zoonotic infection and threat to important and endangered predators may be mitigated through regional rabies control primarily in domestic dogs and cats, as well as by preventative vaccination of at-risk park employees and their pets. The importance of ongoing prophylactic rabies protection by regular vaccination of highly endangered wildlife carnivores and the submission of carcasses for rabies diagnosis of any wild or domestic animals behaving uncharacteristically or found dead is emphasised.

Social media as a new phenomenon has become a tool used by many televangelists and pastors all over the world. It is against this background that this research sought to explore the Facebook activities of some Ghanaian Pentecostal pastors from a missional perspective. The article deals with the concept of social media, Facebook and its potential for mission purposes, the Facebook phenomenon among Ghanaians and how Ghanaian Pentecostal Pastors are using Facebook for missional purposes, as well as some of concerns on the negative uses of social media. The study revealed that Ghanaian pastors are followed by people from different religious and societal backgrounds. It has also offered the pastors and their congregations the opportunity to form relationships with a wide and diverse range of people without being bound by geographical space.

Sosiale media as nuwe fenomeen het 'n instrument geword wat deur meeste TV-evangeliste en pastore regoor die wêreld gebruik word. Dit is teen hierdie agtergrond dat die Facebook-aktiwiteite van sommige Ghanese Pentekostale pastore vanuit 'n missionale perspektief ondersoek word. In hierdie artikel word die volgende ondersoek: Die konsepte van sosiale media en Facebook, veral dan in terme van hulle potensiaal vir missionale doeleindes; die Facebook-fenomeen, spesifiek onder die Ghanese; en die wyse waarop Ghanese Pentekostale pastore van Facebook gebruik maak vir missionale doeleindes. Die navorsing bevind dat Ghanese pastore deur mense van verskillende godsdienstige en sosiale agtergronde op sosiale media gevolg word. Dit bied aan pastore en hulle geloofsgemeenskappe die geleentheid om verhoudings met 'n wye verskeidenheid mense te ontwikkel, sonder om deur die grense van geografiese gesitueerdheid beperk te word.

OBJETIVO: Validar um modelo de obesidade induzida por dieta hiperlipídica, de baixo custo, fácil reprodutibilidade, que mimetizasse características observadas no humano e viabilizasse posteriores proposições terapêuticas. MATERIAIS E MÉTODOS: Dezesseis camundongos Swiss receberam dieta padrão (DP) ou dieta hiperlipídica (DH), durante 10 semanas. RESULTADOS: Embora o grupo DP tenha apresentado maior consumo de água (p < 0,01) e ração (p < 0,001), o grupo DH apresentou maior ganho de peso corpóreo (p < 0,5) e aumento de coxins adiposos (p < 0,001), favorecendo maior índice de adiposidade (p < 0,001), glicemia (p < 0,01) e área sob a curva nos testes de tolerância à insulina (p < 0,001) e à glicose (p < 0,01). CONCLUSÃO: Validou-se um modelo de obesidade induzida por dieta hiperlipídica associada à resistência à ação da insulina e à intolerância à glicose, em um período de 10 semanas.

OBJECTIVE: Validate a model of high-fat diet-induced obesity, of low cost, easy reproducibility, that could express characteristics observed in human, and would enable subsequent therapy proposals. MATERIALS AND METHODS: Sixteen Swiss mice received a standard diet (DP) or high-fat diet (DH) for 10 weeks. RESULTS: Although the DP group had greater water (p < 0.01) and feed (p < 0.001) consumption, the DH group had greater body weight (p < 0.5) and adipose tissue gain (p < 0.001), favoring higher adiposity index (p < 0.001), glucose (p < 0.01), and area under the curve in the insulin (p < 0.001) and glucose (p < 0.01) tolerance tests. CONCLUSION: A high-fat diet-induced obesity model has been validated, which was also associated with insulin resistance and glucose intolerance after a period of 10 weeks.

PURPOSE: Cardiopulmonary bypass is known to alter propofol pharmacokinetics in patients undergoing cardiac surgery. However, few studies have evaluated the impact of these alterations on postoperative pharmacodynamics. This study was designed to test the hypothesis that changes in propofol pharmacokinetics increase hypnotic effects after cardiopulmonary bypass. METHODS: Twenty patients scheduled for on-pump coronary artery bypass graft (group, n=10) or off-pump coronary artery bypass graft (group, n=10) coronary artery bypass grafts were anesthetized with sufentanil and a propofol target controlled infusion (2.0 µg/mL). Depth of hypnosis was monitored using the bispectral index. Blood samples were collected from the induction of anesthesia up to 12 hours after the end of propofol infusion, at predetermined intervals. Plasma propofol concentrations were measured using high-performance liquid chromatography, followed by a non-compartmental propofol pharmacokinetic analysis. Data were analyzed using ANOVA, considering p<0.05 as significant. RESULTS: After cardiopulmonary bypass, despite similar plasma propofol concentrations in both groups, bispectral index values were lower in the on-pump coronary artery bypass graft group. Time to extubation after the end of propofol infusion was greater in the on-pump coronary artery bypass graft group (334 ± 117 vs. 216 ± 85 min, p = 0.04). Patients undergoing cardiopulmonary bypass had shorter biological (1.82 ± 0.5 vs. 3.67 ± 1.15h, p < 0.01) and terminal elimination (6.27 ± 1.29 vs. 10.5h ± 2.18, p < 0.01) half-life values, as well as higher total plasma clearance (28.36 ± 11.40 vs.18.29 ± 7.67 mL/kg/min, p = 0.03), compared to patients in the off-pump coronary artery bypass graft group. CONCLUSION: Aside from the increased sensitivity of the brain to anesthetics after cardiopulmonary bypass, changes in propofol pharmacokinetics may contribute to its central nervous system effects.

The accurate identification of wound infection provides a clinical challenge to those involved in patient care and would appear to have not kept pace with other developments in the field of wound care. Attempts have been made to improve on the situation but diverse opinions together with lack of consensus prevail. The literature on infection criteria was reviewed and a set of criteria were collated; a number of criteria at the time were described as a subtle in nature and have undergone validation studies. This article suggests a new perspective on the clinical identification of wound infection - ie, the signs of infection are closely associated with the wound type. Infection criteria for six wound types are explored and presented. This approach is intended for consideration together with the results of an impending Delphi study that raise awareness of this issue, promote discussion, and lead to validation of an accurate set of clinical criteria.

La correcta identificación de la infección presente en las heridas representa un reto clínico para los profesionales de la atención de enfermos y parece no haberse desarrollado a la par que otros avances en el campo del tratamiento de heridas. Ha habido intentos para que la situación mejore, pero al final siguen prevaleciendo las diferencias de opinión y la falta de consenso. Se han revisado las teorías acerca de las causas de las infecciones y se ha recopilado un buen número de ellas. Algunas de las teorías que en su tiempo se consideraron acertadas han sido sometidas a estudios de comprobación. Este artículo propone un nuevo enfoque de la identificación clínica de las infecciones en las heridas (es decir, de las señales de infección estrechamente relacionadas con los diferentes tipos de heridas). En este trabajo vamos a presentar y desarrollar las teorías de aparición de infecciones en seis tipos diferentes de heridas. El presente estudio está relacionado con los resultados de una investigación que, en breve, un foro Delphi va a publicar y que contribuirán a reflexionar sobre este tema, promover el debate y llegar a validar un grupo concreto de criterios clínicos.

OBJECTIVE: Inadequate water and sanitation services adversely affect the health and socioeconomic development of communities. The Water and Sanitation Extension Programme (WASEP) project, undertaken in selected villages in northern Pakistan between 1997 and 2001, was designed to deliver an integrated package of activities to improve potable water supply at village and household levels, sanitation facilities and their use, and awareness and practices about hygiene behaviour. METHODS: A case-control study was conducted during July-September 2001 to evaluate whether, after selected confounders were controlled for, children aged <6 years with diarrhoea were more or less likely to reside in villages that participated in the project than in villages that did not participate. Descriptive and logistic regression analyses were performed. FINDINGS: Children not living in WASEP villages had a 33% higher adjusted odds ratio for having diarrhoea than children living in WASEP villages (adjusted odds ratio, 1.331; P<0.049). Boys had 25% lower odds of having diarrhoea than girls (adjusted odds ratio, 0.748; P<0.049). A 2.6% decrease was found in the odds of diarrhoea for every yearly increase in the mother's age (adjusted odds ratio, 0.974; P<0.044) and a 1.4% decrease for every monthly increase in the child's age (adjusted odds ratio, 0.986; P<0.001). CONCLUSIONS: The findings in this study may help refine the approach to future water, sanitation, and hygiene initiatives in northern Pakistan. The integrated approach taken by WASEP, which incorporates engineering solutions with appropriate education to maximize facility usage and improve hygiene practices, is a useful example of how desired health benefits can be obtained from projects of this type.

OBJETIVO: Los servicios inadecuados de agua y saneamiento perjudican a la salud y el desarrollo socioeconómico de las comunidades. El proyecto Programa de Extensión de Agua y Saneamiento (WASEP), emprendido en determinadas aldeas del norte del Pakistán entre 1997 y 2001, se concibió para hacer llegar un paquete integrado de actividades encaminadas a mejorar el suministro de agua potable a las aldeas y hogares, las instalaciones de saneamiento y el uso de las mismas, y las prácticas higiénicas y la sensibilización al respecto. MÉTODOS: Durante los meses de julio a septiembre de 2001 se emprendió un estudio de casos y controles para evaluar si, después de controlar el efecto de algunas variables de confusión, los niños menores de 6 años con diarrea tenían más o menos probabilidades de residir en aldeas que participaban en el proyecto que en aldeas que no lo hacían. Se efectuaron análisis descriptivos y de regresión logística. RESULTADOS: Los niños que no vivían en aldeas abarcadas por el proyecto tenían una razón de posibilidades ajustada de padecer diarrea superior en un 33% a la de los niños que vivían en las aldeas abarcadas por él (razón de posibilidades ajustada, 1,331; P < 0,049). Los niños tenían un 25% menos de probabilidades de padecer diarrea que las niñas (razón de posibilidades ajustada, 0,748; P < 0,049). Se observó una disminución del 2,6% de las probabilidades de sufrir diarrea por cada año adicional de la edad de la madre (razón de posibilidades ajustada, 0,974; P < 0,044) y una disminución del 1,4% por cada mes adicional de la edad del niño (razón de posibilidades ajustada, 0,986; P < 0,001). CONCLUSIÓN: Los resultados de este estudio pueden ayudar a enfocar mejor las futuras iniciativas en materia de agua, saneamiento e higiene en el norte del Pakistán. El enfoque integrado adoptado por el WASEP, que complementa las soluciones técnicas con una educación apropiada para maximizar el uso de las instalaciones y mejorar las prácticas de higiene, es un valioso ejemplo de las posibilidades de mejora de la salud que brindan los proyectos de ese tipo.

OBJECTIF: L'insuffisance des services d'approvisionnement en eau et d'assainissement nuit à la santé et au développement socio-économique des communautés. Le projet WASEP (Water and Sanitation Extension Programme), mis en œuvre dans quelques villages du nord du Pakistan entre 1997 et 2001, visait à offrir un ensemble cohérent d'activités destinées à améliorer l'approvisionnement des villages et des ménages en eau potable, les installations d'assainissement et leur utilisation et les connaissances et pratiques des communautés en matière de comportements liés à l'hygiène. MÉTHODES: Une étude cas-témoins a été réalisée de juillet à septembre 2001 pour examiner si, après exclusion de certains facteurs de confusion, les enfants de moins de 6 ans atteints de diarrhée avaient une plus grande probabilité de résider dans les villages couverts par le projet que dans des villages non couverts. Des analyses descriptives et des analyses par régression logistique ont été effectuées. RÉSULTATS: En ce qui concerne la diarrhée, les enfants ne résidant pas dans les villages couverts par le WASEP avaient un odds ratio ajusté supérieur de 33 % à celui des enfants résidant dans les villages couverts par le projet (odds ratio ajusté : 1,331 ; p<0,049). La probabilité d'avoir la diarrhée était plus faible de 25 % chez les garçons que chez les filles (odds ratio ajusté : 0,748 ; p<0,049). Elle présentait une relation inverse avec l'âge de la mère et de l'enfant, diminuant de 2,6 % par année d'âge de la mère (odds ratio ajusté : 0,974 ; p<0,044) et de 1,4 % par mois d'âge de l'enfant (odds ratio ajusté : 0,986 ; p<0,001). CONCLUSION: Les résultats de cette étude peuvent servir à affiner l'approche des initiatives futures en matière d'eau, d'assainissement et d'hygiène dans le nord du Pakistan. L'approche intégrée adoptée par le projet WASEP, qui allie des solutions techniques à une éducation appropriée en vue de maximiser l'utilisation des installations et d'améliorer les pratiques d'hygiène, constitue un exemple utile de la façon dont les avantages recherchés sur le plan de la santé peuvent être obtenus grâce à des projets de ce type.