RESUMO: Rhododendron fortunei pertence a uma espécie de rododendro perfumada nativa da China, que produz flores perfumadas de grande valor ornamental e ambiental para paisagismo ou embelezamento de interiores. No entanto, os aromas em Rhododendron fortunei ainda não foram investigados, muito menos o mecanismo de formação dessas fragrâncias nas flores. Neste estudo, medimos os aromas em termos de seus componentes e conteúdos voláteis (VOC) em Rhododendron fortunei em quatro diferentes estágios de floração e em diferentes tecidos da planta por microextração em fase sólida headspace combinada com cromatografia gasosa-espectrometria de massa. Em seguida, foram calculados os valores aromáticos característicos, que refletem o grau de percepção olfativa pelo ser humano, de cada VOC na planta de acordo com seus respectivos limiares aromáticos. Os resultados mostraram que três COVs principais medidos do mais alto ao mais baixo são benzoatos de metila, terpenos e derivados de ácidos graxos. Seu conteúdo aumentou após a abertura do botão floral e atingiu o máximo na metade da floração total. Em uma flor, a maioria dos teores de COV foram medidos em pétalas e apenas traços em outros tecidos, como estame, pistilo. Uma pequena quantidade de COVs foi determinada nas folhas também. Todos os valores aromáticos foram quase correspondentes aos teores de três COVs principais, indicando que a fragrância da flor surge verdadeiramente desses componentes de COV. Para entender o mecanismo de formação deste tipo principal de fragrância e sua regulação, primeiramente isolamos um gene de RfBAMT da pétala de Rhododendron fortunei usando clonagem homóloga e tecnologia RACE. O comprimento total de seu cDNA era de 1383 bp, com um quadro de leitura aberto de 1104 bp, codificando um total de 368 aminoácidos. A análise da árvore filogenética mostrou que RfBAMT foi o mais próximo do BSMT de Camellia japonica, pertencente à família das metiltransferases. Em seguida, medimos o nível de expressão de RfBAMT novamente em quatro estágios de desenvolvimento da flor e em diferentes tecidos e folhas de flores. Os resultados mostraram que o nível de expressão deste gene foi altamente correlacionado positivamente com o conteúdo emitido de benzoatos de metila na floração, implicando que RfBAMT desempenha um papel fundamental na formação e regulação de benzoatos de metila em Rhododendron fortune foi clonado e identificado em nosso estudo e seu nível de expressão foi altamente correlacionado positivamente com o conteúdo emitido de benzoatos de metila nas flores e folhas, o que indicou que este gene pode desempenhar um papel importante na regulação da síntese de benzoato de metila em Rhododendron fortunei. RESUMO China interiores entanto investigados (VOC gasosaespectrometria gasosa espectrometria massa seguida característicos humano graxos estame pistilo também RACE 138 bp 110 36 aminoácidos japonica metiltransferases 13 11 3 1

ABSTRACT: Rhododendron fortune belongs to a scented Rhododendron species native to China, which produces fragrant flowers of great ornamental and environmental values for landscaping or indoor beautification. However, the scents in Rhododendron fortuneihave not yet been investigated, let alone the mechanism of the formation of these fragrance in the flowers. In this study, we measured the scents in terms of its volatile components and contents (VOC) in Rhododendron fortuneiat four different flowering stages and in different tissues of the plant by headspace solid-phase micro-extraction combined (HS-SPME) with gas chromatography-mass spectrometry (GC-MS). Then the characteristic aromatic values, which reflects the degree of scent perception by human, of each VOC in the plant was calculated according to its respective aromatic thresholds. Results showed that three main VOCs measured from highest to lowest are methyl benzoates, terpenes and fatty acid derivatives. Their content increased after the flower bud opening and reached the highest at half to full blossom. In a flower most VOC contents were measured in petals and only trace amount in other tissues such as stamen, pistil. A small amount of VOCs was determined in leaves as well.All aromatic values were almost corresponded to the contents of three main VOCs, indicating that the flower fragrance arises truly from these VOC components. S-adenosyl-L-methionine: benzoic acid carboxyl methyl transferase (BAMT) catalyzes the final step to form methyl benzoates. To understand the mechanism of the formation of this main type fragrance and its regulation, we firstly isolate a gene of RfBAMT from petal of Rhododendron fortuneiby using homologous cloning and RACE technology. The full length of its cDNA was 1383 bp，with an open reading frame of 1104 bp, encoding a total of 368 amino acids. The phylogenetic tree analysis showed that RfBAMT was the closest to the BSMT of Camellia japonica, belonging to methyltransferases family. Then we measured the expression level of RfBAMT again at four flower developmental stages and in different flower tissues and leaves. The results showed that the expression level of this gene was highly positively correlated with the emitted content of methyl benzoates in the flowering, implying that RfBAMT plays a pivotal role in the formation and regulation of methyl benzoates in Rhododendron fortune.Thisresearchshowed that the RfBAMT was cloned and identified in our study and its expression level was highly positively correlated with the emitted content of methyl benzoates in the flowers and leaves, which indicated this gene may play an important role on regulation of methyl benzoate synthesis in Rhododendron fortunei. ABSTRACT China beautification However investigated (VOC solidphase solid phase microextraction micro extraction HSSPME HS SPME (HS-SPME chromatographymass chromatography mass GCMS. GCMS GC MS . (GC-MS) human thresholds derivatives blossom stamen pistil wellAll well All SadenosylLmethionine S adenosyl L methionine S-adenosyl-L-methionine BAMT (BAMT technology 138 bpwith bp 110 36 acids japonica family fortuneThisresearchshowed Thisresearchshowed fortunei (GC-MS 13 11 3 1

Abstract Pulmonary mesenchymal stem cells (PMSCs) are considered important in therapeutic and regenerative responses to lung injury. Despite extensive studies on the human, porcine, sheep, mouse and rat, it was found that PMSCs, which can been performed on goose, have not been well explored. Elucidation of cell differentiation, proliferation, which have not yet been well described, may promote the development of injured lung therapy. In our study, we firstly disclosed biological characteristics of spindle-shaped PMSCs from pulmonary tissues of goose embryos. Growth kinetics and counting kit-8 (CCK8) assay were employed for proliferative activity evaluation. When appropriately induced, PMSCs could differentiate into osteoblasts, adipocytes, chondrocytes, type II alveolar epithelial cells (ATII), which demonstrated that PMSCs have cross-embryonic layer differentiation potential. Besides, undifferentiated PMSCs consistently expressed MSCs associated phenotypes, such as CD166, CD90, CD44, CD29, CD71 and CD105, as identified by immunohistochemistry and RT-PCR. Karyotype analysis demonstrated that PMSCs possessed stable diploid karyotype. Collectively, we successfully established applicable methods for isolation, culture, identification and characterization of goose PMSCs which suggested a potential therapeutic use in regenerative therapy. (PMSCs injury human porcine sheep rat explored proliferation described therapy study spindleshaped spindle shaped embryos kit8 kit 8 kit- CCK8 CCK (CCK8 evaluation induced osteoblasts adipocytes chondrocytes ATII, ATII , (ATII) crossembryonic cross embryonic Besides phenotypes CD166 CD CD90 CD44 CD29 CD7 CD105 RTPCR. RTPCR RT PCR. PCR RT-PCR karyotype Collectively isolation culture (CCK (ATII CD16 CD9 CD4 CD2 CD10 CD1

Silicon catalysts have very low catalytic activity in polyester (PET). However, titanium catalysts have high catalytic activity in polyester. The simultaneous use of titanium and silicon catalysts is effective in reducing the activity of a single titanium catalytic base agent. In addition, the glycol salts of titanium and silicon (Ti-EG and Si-EG) prepared in the present invention for use in the polyester catalytic process can avoid the introduction of other groups of impurities during the polyester catalytic process, which would lead to premature capping of the polyester in the process of polycondensation and chain building and make it difficult to increase the molecular weight, and thus affect the quality of the polyester. It has been verified that the ratio of Ti:Si is 1:1 (mol), and total amount added to the catalyst is 0.15% of the raw material amount, and the polycondensation temperature was 260-280 °C for 60 min. Compared with the single Ti-EG catalyst, the b* value of the synthesized PET was significantly reduced, and the molecular weight and other indexes did not change too much. In addition, at the end of catalysis, the catalyst was uniformly dispersed in the reaction system, which could be used as a matting agent for PET. . (PET) However addition TiEG Ti EG SiEG Si Si-EG TiSi 11 1 1: mol, mol , (mol) 015 0 15 0.15 260280 260 280 260-28 C 6 min b reduced much catalysis system (PET (mol 01 0.1 26028 26 28 260-2 0. 2602 2 260-

RESUMO Objetivos: A osteoartrite (OA) é uma doença degenerativa acompanhada de lesão dos condrócitos. Este estudo examinou a desregulação do microRNA-650 (miR-650) nos tecidos da cartilagem de doentes com OA. A sua função e mecanismo também foram explorados em modelos celulares de OA. Métodos: Os níveis de miR-650 foram examinados em 15 tecidos de cartilagem de OA e em 10 tecidos de cartilagem normal saudável. As células SW1353 foram utilizadas para experiências de função celular, e a IL-1β atua sobre as células para imitar as condições da OA in vitro. Foram detectadas funções celulares, incluindo a proliferação, a apoptose e a inflamação. O gene alvo a jusante do miR-650 foi reconhecido e confirmado por meio de análise bioinformática e ensaio de atividade da luciferase. Foram efetuadas experiências de recuperação para verificação do mecanismo. Resultados: Foi testada uma expressão oprimida do miR-650 tanto em doentes com OA como em modelos celulares. A sobreexpressão do miR-650 aumentou a proliferação celular, mas suprimiu a apoptose e a inflamação da SW1353. Como gene alvo do miR-650, a sobreexpressão do WNT1 contrariou o papel do miR-650 na função do SW1353. Conclusão: O miR-650 pode proteger contra a lesão da cartilagem articular na OA através da ação sobre o WNT1. Nível de Evidência I, Estudo de Experimental. Objetivos (OA condrócitos microRNA650 microRNA 650 microRNA-65 miR650 miR (miR-650 Métodos miR-65 1 saudável SW SW135 celular IL1β ILβ IL 1β β vitro luciferase Resultados miR650, 650, WNT Conclusão I Experimental microRNA65 65 microRNA-6 miR65 (miR-65 miR-6 SW13 microRNA6 6 microRNA- miR6 (miR-6 miR- SW1 (miR- (miR

ABSTRACT Objectives: Osteoarthritis (OA) is a degenerative disease associated with chondrocyte injury. This study investigated the dysregulation of microRNA-650 (miR-650) in cartilage tissues of patients with OA. Its function and mechanism were also investigated in OA cell models. Methods: miR-650 levels were examined in 15 OA cartilage tissues and ten healthy cartilage tissues. SW1353 cells were used for cell function experiments and IL-1β was applied to the cells to mimic OA conditions in vitro. Cell functions such as proliferation, apoptosis, and inflammation were detected. The downstream target gene of miR-650 was identified and confirmed by bioinformatic analysis and luciferase activity assay. Rescue experiments were performed to verify the mechanism. Results: Suppressed expression of miR-650 was tested in patients with OA and cell models. Overexpression of miR-650 increased cell proliferation but suppressed apoptosis and inflammation of SW1353. As the target gene of miR-650, WNT1 overexpression counteracted the role of miR-650 in the function of SW1353. Conclusion: miR-650 can protect against articular cartilage injury in OA by targeting WNT1. Level of Evidence I, Experimental Study. Objectives (OA microRNA650 microRNA 650 microRNA-65 miR650 miR (miR-650 models Methods miR-65 1 SW SW135 IL1β ILβ IL 1β β vitro detected assay Results miR650, 650, WNT Conclusion I Study microRNA65 65 microRNA-6 miR65 (miR-65 miR-6 SW13 microRNA6 6 microRNA- miR6 (miR-6 miR- SW1 (miR- (miR

Objective: Panic disorder (PD) is a common disabling condition characterized by recurrent panic attacks. Emotional and behavioral impairments are associated with functional connectivity (FC) and network abnormalities. We used whole-brain FC, modular networks, and graph-theory analysis to investigate extensive network profiles in PD. Methods: Functional magnetic resonance imaging (fMRI) data from 82 subjects with PD and 97 healthy controls were included. Intrinsic FC between each pair of 160 regions, six intra-network, and 15 inter-network FCs were analyzed. Topological properties were explored. Results: PD patients showed altered FCs within the right insula, between frontal cortex-posterior cingulate cortex (PCC), frontal cortex-cerebellum, and PCC-occipital cortex (corrected p < 0.001). Lower connections within the sensorimotor network (SMN) and SMN-occipital network (OCN) were detected (p < 0.05). Various decreased global and local network features were found in PD (p < 0.05). In addition, significant correlations were found between PD symptoms and nodal efficiency (Ne) in the insula (r = -0.273, p = 0.016) and intra-insula FC (r = -0.226, p = 0.041). Conclusion: PD patients present with abnormal functional brain networks, especially decreased FC and Ne within the insula, suggesting that dysfunction of information integration plays an important role in PD. Objective (PD attacks (FC abnormalities wholebrain whole networks graphtheory graph theory Methods fMRI (fMRI 8 9 included 16 regions intranetwork, intranetwork intra network, intra-network 1 internetwork inter analyzed explored Results cortexposterior posterior PCC, PCC , (PCC) cortexcerebellum, cortexcerebellum cerebellum, cerebellum cortex-cerebellum PCCoccipital occipital corrected 0.001. 0001 0.001 . 0 001 0.001) SMN (SMN SMNoccipital OCN (OCN 0.05. 005 0.05 05 0.05) addition (Ne r 0.273, 0273 273 -0.273 0.016 0016 016 intrainsula 0.226, 0226 226 -0.226 0.041. 0041 0.041 041 0.041) Conclusion (PCC 000 0.00 00 0.0 0.273 027 27 -0.27 0.01 01 0.226 022 22 -0.22 004 0.04 04 0. 0.27 02 2 -0.2 0.22 0.2 -0. -0 -

Abstract Objectives To determine whether tinnitus negatively impacts the accuracy of sound source localization in participants with normal hearing. Methods Seventy-five participants with tinnitus and 74 without tinnitus were enrolled in this study. The accuracy of sound source discrimination on the horizontal plane was compared between the two participant groups. The test equipment consisted of 37 loudspeakers arranged in a 180° arc facing forward with 5° intervals between them. The stimuli were pure tones of 0.25, 0.5, 1, 2, 4, and 8 kHz at 50 dB SPL. The stimuli were divided into three groups: low frequency (LF: 0.25, 0.5, and 1 kHz), 2 kHz, and high frequency (HF: 4 and 8 kHz) stimuli. Results The Root Mean Square Error (RMSE) score of all the stimuli in the tinnitus group was significantly higher than that in the control group (13.45 ± 3.34 vs. 11.44 ± 2.56, p = 4.115, t < 0.001). The RMSE scores at LF, 2 kHz, and HF were significantly higher in the tinnitus group than those in the control group (LF: 11.66 ± 3.62 vs. 10.04 ± 3.13, t = 2.918, p = 0.004; 2 kHz: 16.63 ± 5.45 vs. 14.43 ± 4.52, t = 2.690, p = 0.008; HF: 13.42 ± 4.74 vs. 11.14 ± 3.68, t = 3.292, p = 0.001). Thus, the accuracy of sound source discrimination in participants with tinnitus was significantly worse than that in those without tinnitus, despite the stimuli frequency. There was no difference in the ability to localize the sound of the matched frequency and other frequencies (12.86 ± 6.29 vs. 13.87 ± 3.14, t = 1.204, p = 0.236). Additionally, there was no correlation observed between the loudness of tinnitus and RMSE scores (r = 0.096, p = 0.434), and the Tinnitus Handicap Inventory (THI) and RMSE scores (r = −0.056, p = 0.648). Conclusions Our present data suggest that tinnitus negatively impacted sound source localization accuracy, even when participants had normal hearing. The matched pitch and loudness and the impact of tinnitus on patients’ daily lives were not related to the sound source localization ability. Level of evidence 4. hearing Seventyfive Seventy five 7 study groups 3 180 5 them 025 0 25 0.25 05 0.5 SPL LF (LF , (HF (RMSE 13.45 1345 13 45 (13.4 334 34 3.3 vs 1144 11 44 11.4 256 56 2.56 4115 115 4.115 0.001. 0001 0.001 . 001 0.001) 1166 66 11.6 362 62 3.6 1004 10 04 10.0 313 3.13 2918 918 2.918 0.004 0004 004 1663 16 63 16.6 545 5.4 1443 14 43 14.4 452 52 4.52 2690 690 2.690 0.008 0008 008 1342 42 13.4 474 4.7 1114 11.1 368 68 3.68 3292 292 3.292 Thus 12.86 1286 12 86 (12.8 629 6 29 6.2 1387 87 13.8 314 3.14 1204 204 1.204 0.236. 0236 0.236 236 0.236) Additionally r 0096 096 0.096 0.434, 0434 0.434 434 0.434) THI (THI 0056 056 −0.056 0.648. 0648 0.648 648 0.648) patients 18 02 0.2 0. 134 (13. 33 3. 114 11. 2.5 411 4.11 000 0.00 00 116 36 100 10. 31 3.1 291 91 2.91 166 16. 54 5. 144 14. 4.5 269 69 2.69 13. 47 111 329 3.29 12.8 128 (12. 6. 138 120 20 1.20 023 0.23 23 009 09 0.09 043 0.43 005 −0.05 064 0.64 64 (13 2. 41 4.1 0.0 9 2.9 26 2.6 32 3.2 12. (12 1.2 0.4 −0.0 06 0.6 (1 1. −0. ( −0 −

Resumen Antecedentes: El fotosistema II (PSII) es susceptible al estrés por calor. Las plantas acumulan trehalosa para mejorar la tolerancia al estrés, pero se desconoce el mecanismo por el cual la trehalosa afecta al PSII por calor. Preguntas: ¿Cómo afecta la trehalosa al PSII durante estrés por calor? Especie estudiada: Triticum aestivum L. Lugar y fechas del estudio: Shanghái, China. 2019-2021. Métodos: Las plántulas de trigo pretratadas se sometieron a estres por calor y midieron sus parámetros fotosintéticos. Resultados: El estrés por calor provocó reducción en eficiencia fotoquímica de PSII, tasa de transferencia de electrones (ETR(II)), rendimiento cuántico de disipación de energía regulada Y(NPQ) y coeficiente de enfriamiento fotoquímico (qP), pero aumentó rendimiento cuántico de no- disipación de energía regulada de PSII (Y[NO]). Se alteró la forma de la curva de la cinética de inducción de fluorescencia de clorofila rápida (OJIP) y redujó rendimiento máximo de la fotoquímica primaria de PSII (Fv/Fo) y indicador de rendimiento de PSII PIabs. Disminuyeron: actividad de PSII y cadena de transporte de electrones, cantidad de estructuras de hélice α ordenadas y contenido de proteína D1. En trigo pretratado con trehalosa, la proteína D1 y estructuras secundarias de la proteína del PSII se protegieron; mejoraron el transporte de electrones del PSII y de toda la cadena y mantuvieron mayores valores de los parámetros de fluorescencia. Se obtuvieron menores Y(NO) y OJIP más estables. Conclusiones: Trehalosa exógena desempeñó un papel vital en la protección de la función del PSII, generando mayor capacidad fotosintética bajo estrés por calor.

Abstract Background: Photosystem II (PSII) is susceptible to heat stress. Plants naturally accumulate trehalose to improve stress tolerance. However, the mechanism by which trehalose affects PSII during heat stress is still unknown. Questions: How does trehalose affect PSII during heat stress? Studied species: Triticum aestivum L. Study site and dates: Shanghai, China. 2019-2021. Methods: Trehalose -pretreated wheat seedlings suffered from heat stress and their photosynthetic parameters were measured. Results: Heat stress caused a reduction in the photochemical efficiency of PSII, the electron transfer rate (ETR(II)), the quantum yield of regulated energy dissipationY(NPQ) and the coefficient of photochemical quenching (qP), but increased the quantum yield of non-regulated energy dissipation of PSII (Y[NO]). The shape of the fast chlorophyll fluorescence induction kinetics (OJIP) curve in the heat-stressed wheat was altered and the primary photochemistry maximum yield of PSII (Fv/Fo) and the PSII performance indicator PIabs were reduced. Accordingly, the activities of PSII and electron transport chain, the amount of ordered α-helix structures and the content of D1 protein also decreased. However, in trehalose-pretreated wheat, D1 protein and protein secondary structures of PSII were both protected, the electron transport activities of PSII and the whole chain were improved and greater fluorescence parameters values were maintained. Lower Y(NO) and more stable OJIP were obtained. Conclusions: Exogenous trehalose acted a vital role in the protection of the function of PSII, resulting in higher photosynthetic capacity under heat stress.

Abstract Long non-coding RNA AK001796 was initially identified altered in lung cancer. Recent research showed it could participate in the prognosis of hepatocellular carcinoma (HCC). However, the general biological role of AK001796 and its underlying mechanisms in HCC remain unclear. Here we demonstrated that the expression level of AK001796 in HCC tissues and cell lines was up-regulated. Silencing AK001796 suppressed the proliferation ability of HCC cells. Through dual luciferase reporter assays and loss/gain of functions studies, we identified that AK001796 could bind to miR-150, a star microRNA, promoting HCC proliferation. Furthermore, it was reported that growth factor receptor binding protein 2-associated binder 1 (GAB1) is a target gene of miR-150. Owing to AK001796 being a decoy for miR-150 and binding the same putative sites of miR-150 as GAB1, we presented that inhibition of miR-150 in AK001796 silencing cells reversed the reduction in GAB1. Subsequently, our findings demonstrated that silencing AK001796 can impair phospho-ERK1/2 and phospho-AKT. In conclusion, our investigation revealed that AK001796 promoted proliferation by enhancing phospho-ERK1/2 and phospho-AKT through AK001796/miR-150/GAB1 axis in HCC. These results provided further evidence for the critical roles of AK001796 accumulating HCC and suggested that AK001796 might act as an HCC biomarker in clinical treatment. noncoding non coding AK AK00179 cancer . (HCC) However unclear upregulated. upregulated up regulated. regulated up-regulated lossgain loss gain studies miR150, miR150 miR 150, 150 microRNA Furthermore 2associated associated 2 GAB1 GAB (GAB1 miR150. 150. miR-15 Subsequently phosphoERK1/2 phosphoERK12 phosphoERK phospho ERK1/2 ERK1 ERK phospho-ERK1/ phosphoAKT. phosphoAKT AKT. AKT conclusion AK001796/miR150/GAB1 AK001796miR150GAB1 AKmiRGAB AK001796/miR 150/GAB1 AK001796/miR-150/GAB treatment AK0017 (HCC miR15 15 (GAB miR-1 phosphoERK1 phosphoERK1/ ERK12 ERK1/ phospho-ERK1 AK001796/miR150/GAB AK001796miR150GAB AK001796miR AKmiR 150GAB1 150/GAB AK001 miR1 miR- phospho-ERK 150GAB AK00 AK0

Abstract As a natural agricultural product, honey is favored by consumers, and its variety and adulteration have a huge impact on the quality. Acacia honey, red jujube honey and rape honey were used as experimental objects, and their spectral reflectance curves were obtained through a near-infrared spectral image acquisition system. Spectral features were extracted from the preprocessed spectral reflectance curves, and a honey variety classification model based on near-infrared spectral features was established by machine learning. After statistical analysis, Principal Component Analysis Support Vector Machine after processing data through Successive Projections Algorithm (SPA-SVM) is the optimal classification model for three varieties of acacia honey, red jujube honey and rape honey, and the correct rate of honey variety classification reaches 95.83%. The spectral reflectance curve was used to establish a honey adulteration identification model based on the partial least squares-discriiminate analysis (PLS-DA), and the classification accuracy was 97.92% in the test set.

Abstract Objective Oral ulcers are a lesion in the oral mucosa that impacts chewing or drinking. Epoxyeicosatrienoic Acids (EETs) have enhanced angiogenic, regenerative, anti-inflammatory, and analgesic effects. The present study aims to evaluate the effects of 1-Trifluoromethoxyphenyl-3-(1-Propionylpiperidin-4-yl) Urea (TPPU), a soluble epoxide hydrolase inhibitor for increasing EETs level, on the healing of oral ulcers. Methods The chemically-induced oral ulcers were established in Sprague Dawley rats. The ulcer area was treated with TPPU to evaluate the healing time and pain threshold of ulcers. The expression of angiogenesis and cell proliferation-related protein in the ulcer area was detected using immunohistochemical staining. The effects of TPPU on migration and angiogenesis capability were measured with scratch assay and tube formation. Results Compared with the control group, TPPU promoted wound healing of oral ulcers with a shorter healing time, and raised pain thresholds. Immunohistochemical staining showed that TPPU increased the expression of angiogenesis and cell proliferation-related protein with reduced inflammatory cell infiltration in the ulcer area. TPPU enhanced cell migration and tube-forming potential in vitro. Conclusions The present results support the potential of TPPU with multiple biological effects for the treatment of oral ulcers by targeting soluble epoxide hydrolase. drinking (EETs angiogenic regenerative antiinflammatory, antiinflammatory anti inflammatory, anti-inflammatory 1Trifluoromethoxyphenyl31Propionylpiperidin4yl TrifluoromethoxyphenylPropionylpiperidinyl 1 Trifluoromethoxyphenyl 3 Propionylpiperidin 4 yl 1-Trifluoromethoxyphenyl-3-(1-Propionylpiperidin-4-yl TPPU, , (TPPU) level chemicallyinduced chemically induced rats proliferationrelated proliferation related formation group thresholds tubeforming forming vitro (TPPU

ABSTRACT Introduction: Remote ischemic preconditioning (RIPC) is a new noninvasive myocardial protection strategy that uses blood pressure cuf inflation to simulate transient non-fatal ischemia to protect the myocardium and reduce ischemia-reperfusion injury. Sulfonylureas may mask the effects of RIPC due to their cardioprotec-tive effect. This meta-analysis aimed to evaluate whether RIPC, in the absence of sulfonylureas, reduces troponin release in patients undergoing cardiac surgery. Methods: We conducted a meta-analysis of randomized controlled clinical trials to determine whether RIPC can reduce postoperative troponin release in cardiac surgery patients undergoing cardiopulmonary bypass without treatment with sulfonylureas. The data were normalized to equivalent units prior to the analysis. A random-effects model was used to provide more conservative estimate of the effects in the presence of known or unknown heterogeneity. Results: Six studies with a total of 570 participants were included. The analysis showed that troponin release was lower in the RIPC group than in the control group at six hours (test of standardized mean differences = 0, Z=3.64, P<0.001) and 48 hours (Z=2.72, P=0.007) postoperatively. When the mean of cross-clamping time was > 60 minutes, RIPC reduced troponin release at six hours (Z=2.84, P=0.005), 24 hours (Z=2.64, P=0.008), and 48 hours (Z=2.87, P=0.004) postoperatively. Conclusion: In cardiac surgery patients who are not taking sulfonylureas, RIPC can reduce troponin release at six and 48 hours postoperatively; hence, RIPC may serve significant benefits in certain cardiac surgery patients. Introduction (RIPC nonfatal non fatal ischemiareperfusion reperfusion injury cardioprotective cardioprotec tive effect metaanalysis meta sulfonylureas Methods randomeffects random heterogeneity Results 57 included test 0 Z364 Z 3 64 Z=3.64 P<0.001 P0001 P 001 4 Z=2.72, Z272 2 72 (Z=2.72 P=0.007 P0007 007 postoperatively crossclamping cross clamping 6 minutes Z=2.84, Z284 84 (Z=2.84 P=0.005, P0005 P=0.005 , 005 P=0.005) Z=2.64, Z264 (Z=2.64 P=0.008, P0008 P=0.008 008 P=0.008) Z=2.87, Z287 87 (Z=2.87 P=0.004 P0004 004 Conclusion hence 5 Z36 Z=3.6 P<0.00 P000 00 Z=2.72 Z27 7 (Z=2.7 P=0.00 Z=2.84 Z28 8 (Z=2.8 Z=2.64 Z26 (Z=2.6 Z=2.87 Z3 Z=3. P<0.0 P00 Z=2.7 Z2 (Z=2. P=0.0 Z=2.8 Z=2.6 Z=3 P<0. P0 Z=2. (Z=2 P=0. Z= P<0 Z=2 (Z= P=0 P< (Z P=

RESUMEN Introducción: El método de enseñanza por isometría con peso combina los beneficios de la resistencia física y la hipertrofia. Este método de enseñanza realiza el ejercicio isométrico con el peso corporal adecuado. Objetivo: Estudiar los efectos del entrenamiento isométrico con pesas sobre la fuerza explosiva de los miembros inferiores en jugadores de fútbol. Métodos: Se seleccionaron 20 jugadores de fútbol por muestreo aleatorio, divididos por igual en grupo experimental y grupo de control. Se utilizó la situación real de entrenamiento de los atletas. El grupo experimental adoptó el método de enseñanza por isometría con peso, mientras que el grupo de control utilizó el entrenamiento de fuerza muscular convencional. Se comparó la fuerza de las extremidades inferiores de los dos grupos de atletas antes y después del experimento. Resultados: La tasa de crecimiento de la fuerza extensora en los grupos experimentales aumentó significativamente a velocidades medias, altas y bajas, mientras que el par máximo no mostró cambios significativos. Los jugadores de fútbol mejoraron su fuerza muscular. Hubo una diferencia significativa entre los dos grupos (P<0,05). Conclusión: El entrenamiento isométrico con pesas mejora significativamente la fuerza de los músculos extensores medios y rápidos en jóvenes futbolistas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción hipertrofia adecuado Objetivo Métodos 2 aleatorio convencional experimento Resultados medias bajas significativos P<0,05. P005 P P<0,05 . 0 05 (P<0,05) Conclusión futbolistas II tratamiento P00 P<0,0 (P<0,05 P0 P<0, (P<0,0 P<0 (P<0, P< (P<0 (P< (P

RESUMO Introdução: O método de ensino por isometria com peso combina os benefícios de resistência física e hipertrofia. Este método de ensino executa o exercício isométrico com o peso corporal adequado. Objetivo: Estudar os efeitos do treinamento isométrico com pesos sobre a força explosiva dos membros inferiores nos jogadores de futebol. Métodos: Selecionou-se 20 jogadores de futebol por amostragem aleatória, divididos igualmente em grupo experimental e controle. Utilizou-se a situação real de treino dos atletas. O grupo experimental adotou o método de ensino por isometria com peso enquanto o grupo de controle utilizou o treinamento convencional de força muscular. A força dos membros inferiores dos dois grupos de atletas foi comparada antes e após o experimento. Resultado: A taxa de crescimento da força extensora nos grupos experimentais foi significativamente aumentada em velocidades médias, altas e baixas, enquanto o torque de pico não apresentou alterações significativas. Os jogadores de futebol tiveram sua força muscular aprimorada. Houve uma diferença significativa entre os dois grupos (P<0,05). Conclusão: O treinamento isométrico com pesos melhora significativamente a força muscular extensora média e rápida nos jovens jogadores de futebol. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução hipertrofia adequado Objetivo Métodos Selecionouse Selecionou se 2 aleatória Utilizouse Utilizou experimento Resultado médias baixas significativas aprimorada P<0,05. P005 P P<0,05 . 0 05 (P<0,05) Conclusão II tratamento P00 P<0,0 (P<0,05 P0 P<0, (P<0,0 P<0 (P<0, P< (P<0 (P< (P

ABSTRACT Introduction: The isometric weight training method combines the benefits of physical endurance and hypertrophy. This teaching method performs the isometric exercise with the appropriate body weight. Objective: Study the effects of isometric training with weights on soccer players’ explosive strength of the lower limbs. Methods: Twenty soccer players were randomly selected and divided equally into experimental and control groups. The real training situation of the athletes was used. The experimental group adopted the isometrics teaching method with weight, while the control group used conventional muscle strength training. The lower limb strength of the two groups of athletes was compared before and after the experiment. Results: The extensor force growth rate in the experimental groups was significantly increased at medium, high and low speeds, while the peak torque did not show significant changes. The soccer players had their muscle strength improved. There was a significant difference between the two groups (P<0.05). Conclusion: Isometric weight training significantly improves mid and fast-extensor muscle strength in young soccer players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction hypertrophy Objective limbs Methods experiment Results medium speeds changes improved P<0.05. P005 P P<0.05 . 0 05 (P<0.05) Conclusion fastextensor fast II outcomes P00 P<0.0 (P<0.05 P0 P<0. (P<0.0 P<0 (P<0. P< (P<0 (P< (P

ABSTRACT Introduction: Remote ischemic preconditioning (RIPC) is a new noninvasive myocardial protection strategy that uses blood pressure cuf inflation to simulate transient non-fatal ischemia to protect the myocardium and reduce ischemia-reperfusion injury. Sulfonylureas may mask the effects of RIPC due to their cardioprotec-tive effect. This meta-analysis aimed to evaluate whether RIPC, in the absence of sulfonylureas, reduces troponin release in patients undergoing cardiac surgery. Methods: We conducted a meta-analysis of randomized controlled clinical trials to determine whether RIPC can reduce postoperative troponin release in cardiac surgery patients undergoing cardiopulmonary bypass without treatment with sulfonylureas. The data were normalized to equivalent units prior to the analysis. A random-effects model was used to provide more conservative estimate of the effects in the presence of known or unknown heterogeneity. Results: Six studies with a total of 570 participants were included. The analysis showed that troponin release was lower in the RIPC group than in the control group at six hours (test of standardized mean differences = 0, Z=3.64, P<0.001) and 48 hours (Z=2.72, P=0.007) postoperatively. When the mean of cross-clamping time was > 60 minutes, RIPC reduced troponin release at six hours (Z=2.84, P=0.005), 24 hours (Z=2.64, P=0.008), and 48 hours (Z=2.87, P=0.004) postoperatively. Conclusion: In cardiac surgery patients who are not taking sulfonylureas, RIPC can reduce troponin release at six and 48 hours postoperatively; hence, RIPC may serve significant benefits in certain cardiac surgery patients.

ABSTRACT Intercropping has benefited the global agricultural sector in several ways. It is a critical farming practice for long-term productivity, agro-ecological improvement, land management, and land use. It is a well-known method to increase soil health and crop productivity. The impact of intercropping on the yield and production of maize (Zea mays L.) and soybean (Glycine max (L.) Merr.) has been investigated at the leaf, stem, and root level. However, there is limited information about how to determine the best planting density for maize-soybean intercropping (MSI). The benefits and drawbacks of MSI are discussed in this review. Intercropping maize and soybean improves resource use, lodging resistance, and pest and weed control according to the existing research. Furthermore, soybean has low light use efficiency (LUE) and radiation use efficiency (RUE) due to the deep shade created by the maize canopy. As a result, more research is required to discover the optimal planting density to improve light and radiation efficiency.

Abstract Changes in bacterial community composition and bacterial counts of chilled pork during storage at 4 °C were unraveled by culture-dependent method and culture-independent method. Physical and chemical analyses including drip loss, total volatile basic nitrogen (TVB-N), pH, and surface color were also performed to estimate its quality and shelf life. A total of 37 phyla, 575 genera and 843 species were identified in all samples by high-throughput sequencing technology during 10 days’ storage. At the phylum level, Proteobacteria and Firmicutes were the dominant phylum. At the genus level, Pseudomonas spp., Acinetobacter spp., Pantoea spp., Brochothrix spp. and Raoultella spp. were the dominant genera with their average relative abundance above 5%. In addition, 12 species with average relative abundance more than 1% were found. These dominant bacteria were main pathogenic or spoilage bacteria, and seriously affected the quality of chilled meat. Based on the results of total viable counts (TVC), TVB-N level and sensory evaluation, the shelf life of chilled pork stored at 4 °C was no more than 3 days. Through the analyses of the TVC and the microbial community structure during the spoilage of chilled pork, the main microorganisms causing spoilage were revealed, which will guide significance for further control microbial quality of chilled pork.