RESUMO As vilas urbanas são um tipo de área urbana em declínio na China, marcada por aluguéis de baixo custo e por sua localização favorável a uma população flutuante. No entanto, essas vilas apresentam condições de habitação inferiores, externalidades negativas substanciais e um elevado potencial que motiva os governos a desenvolverem ações de requalificação urbana. Este estudo explora o modelo de governança cooperativa entre a requalificação de “vilas urbanas” e a habitação da população flutuante, centrando-se no bem-estar de tais populações por meio de um estudo de caso utilizando dados de entrevistas e análise de cenários. Os resultados observados no estudo de caso mostram que um modo de requalificação adequada tem o potencial de reduzir as dificuldades habitacionais das populações flutuantes, tais como poupar até 154 minutos de tempo de deslocamento por dia de trabalho e 150 dólares em custos mensais de habitação para cada migrante. Finalmente, sugere-se que o modo de requalificação inclusivo seja adotado para todas as futuras intervenções nas vilas urbanas. Considerando as diferentes características dessas vilas e os desejos dos proprietários, o governo pode seguir o modo de requalificação que envolve “requalificação abrangente + locação unificada + gestão de propriedade” ou “aluguel governamental + sistema de previdência social + locação de habitação pública para arrendamento”.

ABSTRACT Urban villages are declining areas in China that attract floating populations due to low rent and favorable locations. These villages have poor housing conditions and substantial negative externalities, yet they also possess a potential that motivates governments to redevelop them. This research explores a cooperative governance model for urban village redevelopment and floating population housing through a case study focusing on the welfare of these populations. Using data from interviews and scenario analysis, the case study reveals that a proper redevelopment mode could alleviate the floating population’s housing difficulties, such as saving up to 154 minutes of commuting time per workday and reducing monthly housing costs by 150 dollars for each migrant. Finally, the research suggests that an inclusive redevelopment mode should be adopted for all future projects in urban villages. Considering the different characteristics of urban villages and the owner’s wishes, the government could pursue redevelopment based on “comprehensive redevelopment + unitizing leasing + property management” or “government rent + social security system + public rental housing leasing.”

RESUMEN Un tipo de área urbana en declive en China es la aldea urbana, en la que los bajos alquileres y la ubicación ventajosa atraen a la población flotante. Sin embargo, las aldeas urbanas tienen condiciones de vivienda inferiores, externalidades negativas sustanciales y un alto potencial que motiva a los gobiernos a reconstruirlas. Este estudio explora el modelo de gobernanza cooperativa entre la renovación de “aldeas urbanas” y la vivienda para poblaciones flotantes, centrándose en el bienestar de las poblaciones flotantes a través de un estudio de caso que utiliza datos de entrevistas y análisis de escenarios. Descubrimos que un modo de reurbanización adecuado podría aliviar las dificultades de vivienda de la población flotante, como ahorrar hasta 154 minutos de tiempo de viaje por día laboral y 150 dólares en costos mensuales de vivienda para cada migrante. Finalmente, sugerimos que se adopte el modo de reurbanización inclusiva para todas las futuras renovaciones de aldeas urbanas. Y teniendo en cuenta las diferentes características de las aldeas urbanas y los deseos de los propietarios, el gobierno puede seguir el modo de reurbanización de “reurbanización integral + arrendamiento unitario + administración de la propiedad” o “alquiler gubernamental + sistema de seguridad social + arrendamiento de viviendas públicas de alquiler”.

RESUMO A solução fundamental para o problema do entupimento do emissor é explorar o projeto ideal da estrutura do emissor e melhorar sua capacidade anti-entupimento. O objetivo da pesquisa foi selecionar a forma estrutural ideal do caminho de fluxo do emissor em sistemas de irrigação por gotejamento utilizando fontes de água de qualidade inferior, proporcionando suporte teórico para melhorar a capacidade anti-obstrução do próprio emissor e para o projeto de novos produtos de emissores. A seleção da estrutura do caminho do fluxo do emissor não tem sido conduzida com base na determinação do modelo de simulação apropriado com a condição complexa de qualidade da água. Para tanto, determinou-se o modelo de movimento interno do fluido adequado do emissor, e avaliou-se de forma abrangente o desempenho hidráulico e o desempenho anti-entupimento das cinco estruturas representativas da trajetória de fluxo no país e no exterior, e finalmente determinou-se a estrutura ótima da trajetória de fluxo do emissor. Os resultados da pesquisa indicaram que o modelo RNG k-ε foi o mais adequado para simular a área do fluxo no emissor por meio da análise abrangente da lacuna entre os valores simulados e os valores medidos, da precisão do cálculo e da eficiência computacional. A trajetória de fluxo fractal foi relativamente ótima para o desempenho hidráulico e para o desempenho anti-entupimento. Este estudo pode fornecer suporte teórico para melhorar a própria habilidade anti-obstrução do emissor e o projeto de novo produto de emissor. antientupimento. antientupimento anti entupimento. inferior antiobstrução obstrução emissores tanto determinouse determinou se avaliouse avaliou exterior kε k ε medidos computacional

ABSTRACT The fundamental solution to the problem of emitter clogging is to explore the optimal structure design of emitter and improve its anti-clogging ability. The research objective was to select the optimal structural form of the emitter flow path in drip irrigation systems using inferior water sources, which can provide theoretical support for improving the anti-clogging ability of the emitter itself and designing new emitter products. The selection of the flow path structure of the emitter has not been conducted on the basis of determining the appropriate simulation model with the condition of complex water quality. Therefore, the appropriate internal fluid movement model of the emitter was determined, the hydraulic performance and anti-clogging performance of the five nationally and internationally representative flow path structures were comprehensively evaluated, and finally the optimal structure of the emitter flow path was determined. The research results indicated that RNG k - ε model was the most suitable to simulate the flow field in the emitter by comprehensive analysis of the gap between simulated values and the measured values, the calculation accuracy and computational efficiency. The fractal flow path was relatively optimal for hydraulic performance and for anti-clogging performance. This study can provide theoretical support for improving the anti-clogging ability of the emitter itself and the design of the new emitter product. anticlogging anti sources products quality Therefore determined evaluated efficiency product

To rapidly detect different types of methamphetamine in the field, a portable near infrared spectroscopy (NIR) method was used to model and analyze the collected samples. Transmission spectra of 359 methamphetamine crystal samples (content range of 7.5-99.4%) and 187 methamphetamine tablet samples (content range of 2.43-23.91%) were collected, first-order derivatives and the Savitaki-Golay smoothing method were applied for spectral preprocessing. The drug concentrations in the calibration and validation sample sets were analyzed using high-performance liquid chromatography. The partial least squares quantitative analysis models were built, and the quantitative models for methamphetamine crystals were 2.10, 2.12 and 2.19% for root mean square error of cross-validation (RMSECV), root mean square error of validation (RMSEV) and root mean square error of prediction (RMSEP), respectively; in contrast, RMSECV, RMSEV, and RMSEP of methamphetamine tablets were 0.78, 0.80, and 0.82%, respectively. The results of the internal cross-validation and the methodology review show that the measurements of the modeled results are consistent with the true values, and that the model has good stability and can be used for accurate field measurements. Therefore, real time sample analysis can be performed using NIR technology combined with chemometric models, which helps the police to determine the quality of drugs in a timely manner for case analysis. (NIR 35 content 7.599.4% 75994 7.5 99.4% 7 5 99 4 7.5-99.4% 18 2.4323.91% 2432391 2.43 23.91% 2 43 23 91 2.43-23.91% firstorder first order SavitakiGolay Savitaki Golay preprocessing highperformance high performance chromatography built 210 10 2.10 212 12 2.1 219 19 2.19 crossvalidation cross RMSECV , (RMSECV) RMSEV (RMSEV RMSEP, (RMSEP) respectively contrast 078 0 78 0.78 080 80 0.80 082 82 0.82% values Therefore 3 599 7.599.4 7599 75 7. 994 99.4 9 7.5-99.4 1 4323 2.4323.91 243239 243 2.4 2391 23.91 2.43-23.91 21 2. (RMSECV (RMSEP 07 0.7 08 8 0.8 0.82 59 7.599. 759 99. 7.5-99. 432 2.4323.9 24323 24 239 23.9 2.43-23.9 0. 7.599 7.5-99 2.4323. 2432 23. 2.43-23. 7.59 7.5-9 2.4323 2.43-23 7.5- 2.432 2.43-2 2.43-

A direct, high-throughput and accurate method, multi-molecular infrared spectroscopic (MMIR) data fusion, was established for the detection of trace of estrone, diethylstilbestrol, betamethasone and prednisone acetate in peptide powders based on near-infrared and mid-infrared fusion datasets. Tri step MMIR with progressively improved resolution captured key fingerprint features of hormones in the regions of 3430-3250, 2950-2850, 1750-1550 and 1335-1050 cm-1 which were exclusively selected as the datasets. The back propagation neural network (BPNN) model with the best performance used a mid-level fusion strategy and achieved excellent root mean square error of prediction (RMSEP, 1.101 mg kg-1) and residual prediction deviation (RPD, 9.890). Compared to those of single dataset models, the RMSEP was reduced by more than 82.03%. This method had remarkable practical significance for the direct and accurate quality evaluation of peptide powder products with favorable limit of detection (LOD) but without pretreatment and chemical agents, verifying the feasibility of multiple analytical instruments for rapid and comprehensive detection of powdered foods. highthroughput high throughput multimolecular multi molecular (MMIR estrone diethylstilbestrol nearinfrared near midinfrared mid datasets 34303250, 34303250 3430 3250, 3250 3430-3250 29502850, 29502850 2950 2850, 2850 2950-2850 17501550 1750 1550 1750-155 13351050 1335 1050 1335-105 cm1 cm 1 cm- BPNN (BPNN midlevel level RMSEP, (RMSEP 1101 101 1.10 kg1 kg kg-1 RPD, RPD (RPD 9.890. 9890 9.890 . 9 890 9.890) models 8203 82 03 82.03% LOD (LOD agents foods 3430325 343 325 3430-325 2950285 295 285 2950-285 1750155 175 155 1750-15 1335105 133 105 1335-10 110 10 1.1 kg- 989 9.89 89 820 8 0 82.03 343032 34 32 3430-32 295028 29 28 2950-28 175015 17 15 1750-1 133510 13 1335-1 11 1. 98 9.8 82.0 34303 3 3430-3 29502 2 2950-2 17501 1750- 13351 1335- 9. 82. 3430- 2950-

RESUMO Objetivo: Comparar a eficácia do Dynesys e do sistema híbrido no tratamento de pacientes com doença degenerativa lombar multissegmentar (DLD). Métodos: Os pacientes envolvidos neste estudo retrospectivo foram divididos em grupos Dynesys (n = 22) e Híbrido (n = 13). Os desfechos clínicos foram avaliados por meio do Oswestry Disability Index (ODI) e da Escala Visual Analógica (EVA). As avaliações radiológicas incluíram radiografia, ressonância nuclear magnética (RNM) e tomografia computadorizada. Ademais, diferentes complicações foram analisadas. Resultados: No acompanhamento final, o ODI e a EVA de todos os grupos melhoraram (p < 0,05), e houve diminuição da amplitude de movimento (ADM) dos segmentos operacionais. No entanto, o grupo Dynesys preservou uma maior extensão da ADM no acompanhamento final (p < 0,05). A ADM do segmento superior adjacente foi ampliada em ambos os grupos (p < 0,05), enquanto as alturas dos discos foram reduzidas no acompanhamento final (p < 0,05). No entanto, o grupo Dynesys apresentou uma redução mais evidente na altura do disco dos segmentos dinâmicos (p < 0,05). Não houve diferença significativa nas complicações entre esses dois grupos (p > 0,05). Conclusão: Neste estudo, resultados satisfatórios semelhantes foram obtidos em ambos os grupos. Ambos os procedimentos cirúrgicos podem ser empregados como tratamentos eficazes para pacientes de meia-idade e fisicamente ativos com LDD multissegmentar. Nível de Evidência III; Estudo Retrospectivo Comparativo. Objetivo DLD. DLD . (DLD) Métodos n 22 13. 13 13) (ODI EVA. (EVA) radiografia RNM (RNM computadorizada Ademais analisadas Resultados p 0,05, 005 0,05 , 0 05 0,05) (ADM operacionais entanto 0,05. Conclusão meiaidade meia idade III Comparativo (DLD 2 1 (EVA 00 0,0 0,

ABSTRACT Objective: To compare effectiveness of Dynesys and hybrid system in treating patients with multi-segmental lumbar degenerative disease (LDD). Methods: Patients involved in this retrospective study were divided into Dynesys (n = 22) and Hybrid (n = 13) groups. Clinical outcomes were evaluated using Oswestry Disability Index (ODI), and Visual Analogue Scale (VAS). Radiologic evaluations included X-ray, MRI, and CT. Furthermore, different complications were analyzed. Results: At the last follow-up, ODI and VAS of each group were improved (p < 0.05), and the range of motion (ROM) of operating segments decreased. However, Dynesys group preserved a larger extent of ROM at the final follow-up (p < 0.05). ROM of the upper adjacent segment was increased in both groups (p < 0.05), while the disc heights were decreased at the final follow-up (p < 0.05). Besides, Dynesys group had a more obvious decrease in the disc height of dynamic segments (p < 0.05). No significant difference existed in complications between both groups (p > 0. 05). Conclusion: In our study, similar satisfactory results were obtained in both groups. Both surgical procedures can be employed as effective treatments for middle-aged and physically active patients with multi-segmental LDD. Level of Evidence III; Retrospective Comparative Study. Objective multisegmental multi segmental LDD . (LDD) Methods n 22 13 ODI, , (ODI) VAS. (VAS) Xray, Xray X ray, ray X-ray MRI CT Furthermore analyzed Results followup, followup follow up, up p 0.05, 005 0.05 0 05 0.05) (ROM However 0.05. Besides 05. 05) Conclusion middleaged middle aged III Study (LDD 2 1 (ODI (VAS 00 0.0

Abstract Background To explore the median effective dose (ED50) and 95% effective dose (ED95) of remimazolam besylate combined with alfentanil for adult gastroscopy. Methods This prospective studyenrolled 31 patients scheduled to painless gastroscopy at Anhui No. 2 Provincial People's Hospital between April and May, 2022. 5 µg.kg−1 of alfentanil hydrochloride was used for pre-analgesia. The initial single loading dose of remimazolam besylate was 0.12 mg.kg−1, increased or reduced by 0.01 mg.kg−1 for the next patient with modified Dixon sequential method. The modified Observer's Assessment of Alertness/Sedation Scale (MOAA/S) was used to assess sedation. Results Combined with alfentanil, the ED50 of remimazolam besylate was 0.147 mg.kg−1 (95% CI: 0.138-0.160 mg.kg−1) and ED95 0.171 mg.kg−1 (95% CI: 0.159-0.245 mg.kg−1). The induction time after injection of remimazolam besylate was 70 ± 25 s, with the anesthesia recovery time and the observation time in resuscitation room 5.13 ± 2.13 min and 2.32 ± 1.6 min, respectively. Twenty nine patients’ vital signs were within acceptable limits during gastroscopy. Conclusions The ED50 of remimazolam besylate combined with alfentanil for painless gastroscopy was 0.147 mg.kg−1, and the ED95 was 0.171 mg.kg−1. ED (ED50 95 (ED95 3 No Peoples People s May 2022 µgkg1 µgkg µg kg 1 µg.kg− preanalgesia. preanalgesia pre analgesia. analgesia pre-analgesia 012 0 12 0.1 mgkg1 mgkg mg 001 01 0.0 mg.kg− method Observers Observer AlertnessSedation Alertness Sedation MOAA/S MOAAS MOAA S (MOAA/S sedation ED5 0147 147 0.14 (95 CI 0.1380.160 01380160 0.138 0.160 138 160 0.138-0.16 ED9 0171 171 0.17 0.1590.245 01590245 0.159 0.245 159 245 0.159-0.24 . 7 513 13 5.1 213 2.1 232 32 2.3 16 6 1. respectively (ED5 9 (ED9 202 µg.kg 0. 00 mg.kg 014 14 (9 1380 0.1380.16 0138016 0138 0.13 0160 0.16 0.138-0.1 017 17 1590 0.1590.24 0159024 0159 0.15 0245 0.24 15 24 0.159-0.2 51 5. 21 2. 23 (ED 20 ( 0.1380.1 013801 013 016 0.138-0. 0.1590.2 015902 015 024 0.2 0.159-0. 0.1380. 01380 0.138-0 0.1590. 01590 02 0.159-0 0.1380 0.138- 0.1590 0.159-

Abstract Background Hypertrophic scar (HS), a fibroproliferative disorder caused by aberrant wound healing following skin injuries such as burns, lacerations and surgery, is characterized by invasive proliferation of fibroblasts and excessive extracellular matrix (ECM) accumulation. The dysregulation of autophagy is the pathological basis of HS formation. Previously, angiopoietin-2 (ANGPT2) was found to be overexpressed in HS fibroblasts (HSFs) compared with normal skin fibroblasts. However, whether ANGPT2 participates in the process of HS formation and the potential molecular mechanisms are not clear. Objective This study is intended to figure out the role of ANGPT2 and ANGPT2-mediated autophagy during the development of HS. Methods RT-qPCR was used to detect ANGPT2 expression in HS tissues and HSFs. HSFs were transfected with sh-ANGPT2 to knock down ANGPT2 expression and then treated with MHT1485, the mTOR agonist. The effects of sh-ANGPT2 or MHT1485 on the proliferation, migration, autophagy and ECM accumulation of HSFs were evaluated by CCK-8 assay, Transwell assay and western blotting. The expression of PI3K/Akt/mTOR pathway-related molecules (p-PI3K, p-Akt and p-mTOR) was assessed by western blotting. Results ANGPT2 expression was markedly upregulated in HS tissues and HSFs. ANGPT2 knockdown decreased the expression of p-PI3K, p-Akt and p-mTOR. ANGPT2 knockdown activated autophagy and inhibited the proliferation, migration, and ECM accumulation of HSFs. Additionally, the treatment of MHT1485, the mTOR agonist, on ANGPT2-downregulated HSFs, partially reversed the influence of ANGPT2 knockdown on HSFs. Study limitations The study lacks the establishment of more stable in vivo animal models of HS for investigating the effects of ANGPT2 on HS formation in experimental animals. Conclusions ANGPT2 downregulation represses growth, migration, and ECM accumulation of HSFs via autophagy activation by suppressing the PI3K/Akt/mTOR pathway. Our study provides a novel potential therapeutic target for HS. HS, , (HS) burns surgery (ECM Previously angiopoietin2 angiopoietin 2 angiopoietin- ANGPT (ANGPT2 (HSFs However clear ANGPT2mediated ANGPTmediated mediated RTqPCR RT qPCR shANGPT2 shANGPT sh sh-ANGPT MHT agonist MHT148 migration CCK8 CCK 8 CCK- blotting PI3KAktmTOR PIKAktmTOR PI3K Akt PI K pathwayrelated pathway related pPI3K, pPI3K pPIK p PI3K, (p-PI3K pAkt pmTOR p-mTOR p-PI3K pmTOR. mTOR. Additionally ANGPT2downregulated ANGPTdownregulated downregulated animals growth (HS (ANGPT MHT14 KAktmTOR PIK pPI MHT1

Abstract Near infrared spectroscopy (NIRS) combined with various chemometrics methods was tried to identify the fresh tea leaves at different altitudes quickly and nondestructively. Three kinds of samples were collected, then scanning NIRS, conducting spectral preprocessing to remove noise information, using backward interval partial least squares to screen characteristic spectral intervals, going on principal component analysis, respectively. Finally, least squares support vector machine method (LS-SVM) was applied to establish NIRS models, whose robustness was tested by prediction set samples. The best pretreated method was the combination of multivariate scattering correction and the first derivative. Six characteristic spectral intervals were screened, and the corresponding spectral wavenumbers were 4821.2-5091.2 cm-1, 5368.9-5638.8 cm-1, 6190.4-6460.4 cm-1, 7011.9-7281.9 cm-1, 8924.9-9191.1 cm-1 and 9734.9-10000 cm-1. The cumulative contribution rate of the first three principal components was 99.92%. The root mean square error of the cross validation and the determination coefficient of the calibration set model were 0.027 and 0.973, respectively. The root mean square error and the determination coefficient of the prediction set model were 0.034 and 0.968, respectively. The discrimination accuracy in prediction set was 100%. The results showed NIRS combined with LS-SVM can realize fast and nondestructive discrimination of fresh tea leaves at different altitudes.

ABSTRACT Introduction: Postoperative thrombocytopenia is common in cardiac surgery with cardiopulmonary bypass, and its risk factors are unclear. Methods: This retrospective study enrolled 3,175 adult patients undergoing valve surgeries with cardiopulmonary bypass from January 1, 2017 to December 30, 2018 in our institute. Postoperative thrombocytopenia was defined as the first postoperative platelet count below the 10th quantile in all the enrolled patients. Outcomes between patients with and without postoperative thrombocytopenia were compared. The primary outcome was in-hospital mortality. Risk factors of postoperative thrombocytopenia were assessed by logistic regression analysis. Results: The 10th quantile of all enrolled patients (75×109/L) was defined as the threshold for postoperative thrombocytopenia. In-hospital mortality was comparable between thrombocytopenia and non-thrombocytopenia groups (0.9% vs. 0.6%, P=0.434). Patients in the thrombocytopenia group had higher rate of postoperative blood transfusion (5.9% vs. 3.2%, P=0.014), more chest drainage volume (735 [550-1080] vs. 560 [430-730] ml, P<0.001), and higher incidence of acute kidney injury (12.3% vs. 4.2%, P<0.001). Age > 60 years (odds ratio [OR] 2.25, 95% confidence interval [CI] 1.345-3.765, P=0.002], preoperative thrombocytopenia (OR 18.671, 95% CI 13.649-25.542, P<0.001), and cardiopulmonary bypass time (OR 1.088, 95% CI 1.059-1.117, P<0.001) were positively independently associated with postoperative thrombocytopenia. Body surface area (BSA) (OR 0.247, 95% CI 0.114-0.538, P<0.001) and isolated mitral valve surgery (OR 0.475, 95% CI 0.294-0.77) were negatively independently associated with postoperative thrombocytopenia. Conclusion: Positive predictors for thrombocytopenia after valve surgery included age > 60 years, small BSA, preoperative thrombocytopenia, and cardiopulmonary bypass time. BSA and isolated mitral valve surgery were negative predictors. Introduction unclear Methods 3175 3 175 3,17 1 201 30 institute th compared inhospital hospital analysis Results 75×109/L 75109L L 75 109 (75×109/L Inhospital In nonthrombocytopenia non 0.9% 09 0 9 (0.9 vs 06 6 0.6% P=0.434. P0434 P P=0.434 . 434 P=0.434) 5.9% 59 5 (5.9 32 2 3.2% P=0.014, P0014 P=0.014 , 014 P=0.014) 735 (73 5501080 550 1080 [550-1080 56 430730 430 730 [430-730 ml P<0.001, P0001 P<0.001 001 12.3% 123 12 (12.3 42 4 4.2% P<0.001. odds OR [OR 225 25 2.25 95 [CI 1.3453.765, 13453765 1.345 3.765, 345 765 1.345-3.765 P=0.002, P0002 P=0.002 002 P=0.002] 18671 18 671 18.671 13.64925.542, 1364925542 13.649 25.542, 13 649 542 13.649-25.542 1088 088 1.088 1.0591.117, 10591117 1.059 1.117, 059 117 1.059-1.117 (BSA 0247 247 0.247 0.1140.538, 01140538 0.114 0.538, 114 538 0.114-0.538 0475 475 0.475 0.2940.77 0294077 0.294 0.77 294 77 0.294-0.77 Conclusion 317 17 3,1 20 7 10 0.9 (0. 0.6 P043 P=0.43 43 5.9 (5. 3.2 P001 P=0.01 01 73 (7 550108 55 108 [550-108 43073 [430-73 P000 P<0.00 00 12.3 (12. 4.2 22 2.2 3453 1.3453.765 1345376 1345 1.34 3765 3.765 34 76 1.345-3.76 P=0.00 1867 67 18.67 64925 13.64925.542 136492554 13649 13.64 25542 25.542 64 54 13.649-25.54 08 1.08 0591 1.0591.117 1059111 1059 1.05 1117 1.117 05 11 1.059-1.11 024 24 0.24 1140 0.1140.538 0114053 0114 0.11 0538 0.538 53 0.114-0.53 047 47 0.47 2940 0.2940.7 029407 0294 0.29 077 0.7 29 0.294-0.7 31 3, 0. (0 P04 P=0.4 5. (5 3. P00 P=0.0 ( 55010 [550-10 4307 [430-7 P<0.0 12. (12 4. 2. 1.3453.76 134537 134 1.3 376 3.76 1.345-3.7 186 18.6 6492 13.64925.54 13649255 1364 13.6 2554 25.54 13.649-25.5 1.0 1.0591.11 105911 105 111 1.11 1.059-1.1 02 0.2 0.1140.53 011405 011 0.1 053 0.53 0.114-0.5 04 0.4 0.2940. 02940 029 07 0.294-0. P0 P=0. 5501 [550-1 [430- P<0. (1 1.3453.7 13453 1. 37 3.7 1.345-3. 18. 13.64925.5 1364925 136 13. 255 25.5 13.649-25. 1.0591.1 10591 1.1 1.059-1. 0.1140.5 01140 0.5 0.114-0. 0.2940 0.294-0 P=0 [550- [430 P<0 1.3453. 1.345-3 13.64925. 136492 25. 13.649-25 1.0591. 1.059-1 0.1140. 0.114-0 0.294- P= [550 [43 P< 1.3453 1.345- 13.64925 13.649-2 1.0591 1.059- 0.1140 0.114- [55 [4 13.6492 13.649- [5 [

Abstract Apples are the fourth most produced fruit in the world, so it is important to safeguard them from disease damage. Although there are many deep learning-based plant disease detection models, the existing deep learning networks have complex structures and require large amounts of computational resources for support. Lightweight networks such as MobileNet and ShuffleNet designed for mobile devices could achieve better classification and faster recognition on public datasets, but their accuracy rates are not yet up to the requirements of practical applications. To address these problems, we constructed an improved apple leaf disease recognition algorithm based on MobileNetV2. We used feature reuse to improve the network structure, added a dense connection structure to the inverse residual module, and an ECA-Net attention module to increase the model's focus on diseased regions. We trained the improved model on the network on a dataset expanded by a generative adversarial network. The results showed that the improved model had a smaller number of model parameters, only 3.3 M, and a higher accuracy rate of 96.23% compared to Resnet50, ShuffleNet, and MobileNet models. The improved model had only 0.34 M addition in the number of parameters compared with MobileNet-V2, and had a 2.2% improvement in accuracy. world damage learningbased models support datasets applications problems MobileNetV2 MobileNetV ECANet ECA Net s regions 33 3 3. 9623 96 23 96.23 Resnet50 Resnet 034 0 34 0.3 MobileNetV2, V2, V2 V MobileNet-V2 22 2 2.2 962 9 96.2 Resnet5 03 0. MobileNet-V 2. 96.

ABSTRACT Purpose: To investigate the role of puerarin on renal fibrosis and the underlying mechanism in renal ischemia and reperfusion (I/R) model. Methods: Rats were intraperitoneally injected with puerarin (50 or 100 mg/kg) per day for one week before renal I/R. The level of renal collagen deposition and interstitial fibrosis were observed by hematoxylin and eosin and Sirius Red staining, and the expression of α-smooth muscle actin (α-SMA) was examined by immunohistochemical staining. The ferroptosis related factors and TLR4/Nox4-pathway-associated proteins were detected by Western blotting. Results: Puerarin was observed to alleviate renal collagen deposition, interstitial fibrosis and the α-SMA expression induced by I/R. Superoxide dismutase (SOD) activities and glutathione (GSH) level were decreased in I/R and hypoxia/reoxygenation (H/R), whereas malondialdehyde (MDA) and Fe2+ level increased. However, puerarin reversed SOD, MDA, GSH and Fe2+ level changes induced by I/R and H/R. Besides, Western blot indicated that puerarin inhibited the expression of ferroptosis related factors in a dose-dependent manner, which further demonstrated that puerarin had the effect to attenuate ferroptosis. Moreover, the increased expression of TLR/Nox4-pathway-associated proteins were observed in I/R and H/R group, but puerarin alleviated the elevated TLR/Nox4 expression. Conclusions: Our results suggested that puerarin inhibited oxidative stress and ferroptosis induced by I/R and, thus, delayed the progression of renal fibrosis, providing a new target for the treatment of renal fibrosis.

ABSTRACT Introduction: A giant left atrium may cause respiratory dysfunction and hemodynamic disturbance postoperatively. This retrospective study aimed to evaluate clinical effects of surgical left atrial reduction in concomitant cardiac valves operations. Methods: One hundred and thirty-five patients with heart valve diseases and giant left atriums from January 2004 to July 2021 were enrolled into this research. They were divided into the folded group (n=63) and the unfolded group (n=72). Patients in the folded group had undergone cardiac valve operations concomitantly with left atrial reductions. The perioperative characteristics were compared between both groups, and subgroup analysis was performed. Results: There were five deaths in the folded group and 25 deaths in the unfolded group (P<0.001). Complications including pneumonia, sepsis, multiple organs dysfunction syndrome, low cardiac output syndrome, and the use of continuous renal replacement therapy were significantly fewer in the folded group. The receiver operating characteristic curve of left atrial max. diameter predicting mortality was significant (area under the curve=0.878, P=0.005), and the cutoff point was 96.5 mm. The stratified analysis for sex showed that more female patients died in the unfolded group. Logistic regression for mortality showed that the left atrium unfolded, left atrial max. diameter, cardiopulmonary bypass time, and mechanical ventilation time increased the risk of death. Conclusion: Surgical left atrial reduction concomitantly with valves replacement could decrease mortality and was safe and effective in giant left atrium patients.

ABSTRACT Introduction: Postoperative thrombocytopenia is common in cardiac surgery with cardiopulmonary bypass, and its risk factors are unclear. Methods: This retrospective study enrolled 3,175 adult patients undergoing valve surgeries with cardiopulmonary bypass from January 1, 2017 to December 30, 2018 in our institute. Postoperative thrombocytopenia was defined as the first postoperative platelet count below the 10th quantile in all the enrolled patients. Outcomes between patients with and without postoperative thrombocytopenia were compared. The primary outcome was in-hospital mortality. Risk factors of postoperative thrombocytopenia were assessed by logistic regression analysis. Results: The 10th quantile of all enrolled patients (75×109/L) was defined as the threshold for postoperative thrombocytopenia. In-hospital mortality was comparable between thrombocytopenia and non-thrombocytopenia groups (0.9% vs. 0.6%, P=0.434). Patients in the thrombocytopenia group had higher rate of postoperative blood transfusion (5.9% vs. 3.2%, P=0.014), more chest drainage volume (735 [550-1080] vs. 560 [430-730] ml, P<0.001), and higher incidence of acute kidney injury (12.3% vs. 4.2%, P<0.001). Age > 60 years (odds ratio [OR] 2.25, 95% confidence interval [CI] 1.345-3.765, P=0.002], preoperative thrombocytopenia (OR 18.671, 95% CI 13.649-25.542, P<0.001), and cardiopulmonary bypass time (OR 1.088, 95% CI 1.059-1.117, P<0.001) were positively independently associated with postoperative thrombocytopenia. Body surface area (BSA) (OR 0.247, 95% CI 0.114-0.538, P<0.001) and isolated mitral valve surgery (OR 0.475, 95% CI 0.294-0.77) were negatively independently associated with postoperative thrombocytopenia. Conclusion: Positive predictors for thrombocytopenia after valve surgery included age > 60 years, small BSA, preoperative thrombocytopenia, and cardiopulmonary bypass time. BSA and isolated mitral valve surgery were negative predictors.

ABSTRACT Purpose To investigate the relationship between atherosclerotic abdominal aortic aneurysm (AAA) and CXC chemokine receptor type 2 (CXCR2). Methods Mouse AAA model was established by embedding angiotensin-II pump (1000 ng/kg/min) in ApoE-/- mice. Mice were received SB225002, a selective CXCR2 antagonist, for treatment. Blood pressure was recorded, and CXCR2+ macrophages were examined by flow cytometry analysis. Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining was performed to detect cell apoptosis of abdominal aortic aneurysms. Macrophages were isolated from ApoE-/- mice and treated with Ang II and/or SB225002. Dihydroethidium staining was carried out to determine reactive oxygen species (ROS) activity. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the production of IL-1β and TNF-α. The corresponding gene expressions were measured using real-time polymerase chain reaction (PCR), western blot, and immunohistochemistry staining. Results We found that Ang II activated the expression of CXCR2 in monocytes during the formation of AAA. Inhibition of CXCR2 significantly reduced the size of AAA, attenuated inflammation and phenotypic changes in blood vessels. Ang II-induced macrophages exhibited elevated ROS activity, and elevated levels of 1β and TNF-α, which were then partly abolished by SB225002. Conclusions CXCR2 plays an important role in AAA, suggesting that inhibiting CXCR2 may be a new treatment for AAA.