Silicosis is a systemic disease caused by long-term exposure to high concentrations of free silica dust particles in the workplace. It is characterized by a persistent inflammatory response, fibroblast proliferation, and excessive collagen deposition, leading to pulmonary interstitial fibrosis. Epithelial interstitial transformation (EMT) can cause epithelial cells to lose their tight junctions, cell polarity, and epithelial properties, thereby enhancing the properties of interstitial cells, which can lead to the progression of fibrosis and the formation of scar tissue. Integrin 1 (ITGB1) is considered an important factor for promoting EMT and tumor invasion in a variety of tumors and also plays an important role in the progression of fibrotic diseases. Therefore, ITGB1 can be used as a potential target for the treatment of silicosis. In this study, we found that silica exposure induced epithelial-mesenchymal transformation in rats and that the expression of integrin ITGB1 was elevated along with the EMT. We used CRISPR/Cas9 technology to construct integrin ITGB1 knockdown cell lines for in vitro experiments. We compared the expression of the EMT key proteins E-cadherin and vimentin in the ITGB1 knockdown cells and wild-type cells simultaneously stimulated by silica and detected the aggregation point distribution of E-cadherin and vimentin in the cells using laser confocal microscopy. Our results showed that ITGB1 knockout inhibited the ITGB1/ILK/Snail signaling pathway and attenuated the EMT occurrence compared to control cells. These results suggested that ITGB1 is associated with silica-induced EMT and may be a potential target for the treatment of silicosis. longterm long term workplace response proliferation deposition (EMT junctions polarity tissue ITGB (ITGB1 diseases Therefore silicosis study epithelialmesenchymal mesenchymal CRISPRCas9 CRISPRCas CRISPR Cas9 Cas CRISPR/Cas experiments Ecadherin E cadherin wildtype wild type microscopy ITGB1ILKSnail ITGBILKSnail ILK Snail silicainduced (ITGB ILKSnail

ABSTRACT Intelligent apple-harvesting robots use a staggered distribution of branches and leaves during operation, causing problems such as slow motion planning, low operational efficiency, and high path cost for multi-degrees-of-freedom (DOF) harvesting manipulators. This study presents an autonomous apple-harvesting robotic arm-hand composite system that aims to improve the operational efficiency of intelligent harvesting in dwarf anvil-planted apple orchards. The machine vision system for fruit detection uses the deep learning convolutional neural network (CNN) YOLOv7 and RGB-D camera online detection coupling technology to rapidly recognise apples. The spatial depth information of the fruit area was then extracted from the aligned depth image for precise positioning. Coordinate transformation was used to obtain the coordinates of the fruit under the coordinate system of the manipulator. Based on the informed rapid-exploration random tree (Informed-RRT*) algorithm and path-planning model, the identified target apples were harvested without collision path planning. In an apple-harvesting test, the recognition accuracy of the visual system was 89.4%, and the average time to harvest a single apple was 9.69 s, which was 4.8% faster than the mainstream general harvesting technology. Moreover, the harvesting time for a single apple was reduced by 1.7%. Thus, the proposed system enabled accurate and efficient fruit harvesting. appleharvesting operation planning multidegreesoffreedom multi degrees freedom DOF (DOF manipulators armhand arm hand anvilplanted anvil planted orchards CNN (CNN YOLOv RGBD RGB D positioning manipulator rapidexploration rapid exploration InformedRRT* InformedRRT Informed RRT* RRT (Informed-RRT* pathplanning model test 894 89 4 89.4% 969 9 69 9.6 s 48 8 4.8 Moreover 17 1 7 1.7% Thus (Informed-RRT 89.4 96 6 9. 4. 1.7 89. 1.

RESUMO Os robôs de colheita inteligente de maçãs têm uma distribuição escalonada de galhos e folhas durante a operação, o que causa problemas como o planejamento de movimentos lentos, a baixa eficiência operacional e o alto custo de trajetória dos manipuladores de colheita com vários graus de liberdade (DOF). Este artigo apresenta um sistema composto de braço-mão robótico autônomo para colheita de maçãs que visa melhorar a eficiência operacional da colheita inteligente em pomares de maçãs plantadas com anéis anões. O sistema de visão mecânica para detecção de frutas usa a rede neural convolucional de aprendizagem profunda (CNN) YOLOv7 e uma tecnologia de acoplamento de detecção on-line de câmera RGB-D para reconhecer rapidamente as maçãs. Em seguida, as informações de profundidade espacial da área da fruta são extraídas da imagem de profundidade alinhada para um posicionamento preciso. A transformação de coordenadas é usada para obter as coordenadas da fruta no sistema de coordenadas do manipulador. Com base no algoritmo de árvore aleatória de exploração rápida informada (Informed-RRT*) e no modelo de planejamento de caminho, as maçãs-alvo identificadas são colhidas sem planejamento de caminho de colisão. Em um teste de colheita de maçãs, a precisão do reconhecimento do sistema visual foi de 89,4%, e o tempo médio de colheita de uma única maçã foi de 9,69 s, 4,8% mais rápido do que a tecnologia de colheita geral convencional. Além disso, o tempo de colheita de uma única maçã foi reduzido em 1,7%. Assim, o sistema proposto permite uma colheita de frutas precisa e eficiente. operação lentos DOF. DOF . (DOF) braçomão braço mão anões CNN (CNN YOLOv online on line RGBD RGB D seguida preciso manipulador InformedRRT* InformedRRT Informed RRT* RRT (Informed-RRT* maçãsalvo alvo colisão 894 89 4 89,4% 969 9 69 9,6 s 48 8 4,8 convencional disso 17 1 7 1,7% Assim eficiente (DOF (Informed-RRT 89,4 96 6 9, 4, 1,7 89, 1,

Abstract The brewing of Chinese Baijiu is a microbial community fermentation system, in which there is a close and complex relationship between microorganisms and flavor quality, but the specific deep-seated mechanism still needs to be explored and interpreted continuously so far. In this review, Maotai flavor Baijiu was briefly introduced, and the microorganisms mainly affected by flavor substances, the main components of flavor substances, the factors affecting flavor formation and the application ways of functional microorganisms in Maotai flavor Baijiu were summarized. It provides a deeper understanding for the research of others flavor Baijiu.

Abstract This study aimed to investigate the effects of eugenol (Eu) at different concentrations (0, 10, 50, 100, 200, and 300 μM/g protein) on the gel properties and chemical structure changes of porcine myofibrillar protein (MP). The results showed that Eu inhibited the increase of surface hydrophobicity and carbonyl content but did not prevent the oxidation-induced loss of thiol groups. Moreover, Eu intensified the loss of the α-helical conformation as well as the tertiary structure of MP under oxidative stress. The physicochemical changes at 10 and 50 μM/g Eu resulted in a significant enhancement of the gelling ability of MP and enhanced the positive role of oxidation in building elastic gel networks. Conversely, Eu at the concentrations of 100, 200, and 300 μM/g was not conducive to gelling ability, especially at 300 μM/g, and these concentrations were associated with Eu-induced protein conformational changes. (Eu 0, 0 (0 100 200 30 μMg μM g MP. . (MP) oxidationinduced induced groups Moreover αhelical α helical stress 1 5 networks Conversely Euinduced ( 20 3 (MP 2

RESUMEN Introducción: El nivel competitivo del tenis ha aumentado en los últimos años, desafiando a los investigadores en la promoción de un mayor nivel de resistencia y rendimiento de sus practicantes. Objetivo: Analizar los impactos del entrenamiento de Crossfit en el rendimiento de atletas de tenis. Métodos: Este trabajo realizó un experimento de cuatro semanas con 50 tenistas profesionales, introduciendo en el grupo experimental un protocolo basado en el entrenamiento CrossFit. Se analizaron estadísticamente los niveles de resistencia deportiva y rendimiento en competición antes y después del experimento, también se analizó si el entrenamiento de Crossfit podía mejorar eficazmente la resistencia deportiva y el nivel de rendimiento de los tenistas. Resultados: Después de la intervención, el tiempo requerido por los tenistas para despegar y golpear la pelota durante 20 veces en tres pasos después del retroceso se redujo de 71,12 segundos antes del experimento a 60,04 segundos en el grupo experimental. La velocidad lineal y la velocidad diagonal del golpe de derecha también aumentaron un 11,00% y un 6,57%, respectivamente. Siendo que el número de pelotas efectivas y de pelotas precisas en el retiro aumentaron en 5,87% y 5,58%. Conclusión: El entrenamiento de Crossfit puede mejorar la resistencia deportiva de los tenistas, desempeñando un papel positivo en la mejora del nivel de los jugadores. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción años practicantes Objetivo Métodos 5 profesionales CrossFit Resultados intervención 2 7112 71 12 71,1 6004 60 04 60,0 1100 11 00 11,00 657 6 57 6,57% respectivamente 587 87 5,87 558 58 5,58% Conclusión jugadores II tratamiento 711 7 1 71, 600 0 60, 110 11,0 65 6,57 8 5,8 55 5,58 11, 6,5 5, 5,5 6,

RESUMO Introdução: O nível competitivo do tênis tem se elevado nos últimos anos, desafiando os pesquisadores na promoção de um maior nível de resistência e desempenho dos seus praticantes. Objetivo: Analisar os impactos do treinamento de CrossFit sobre o desempenho dos atletas de tênis. Métodos: Este trabalho conduziu uma experiência de quatro semanas com 50 tenistas profissionais, introduzindo um protocolo baseado no treinamento de CrossFit ao grupo experimental. Níveis de resistência esportiva e o desempenho na competição foram analisados estatisticamente antes e depois do experimento, também foi analisado se o treinamento CrossFit poderia efetivamente melhorar a resistência esportiva e o nível de desempenho dos tenistas. Resultados: Após a intervenção, o tempo necessários para os tenistas decolarem e baterem na bola por 20 vezes em três etapas após o recuo foi reduzido de 71,12 segundos antes do experimento para 60,04 segundos, no grupo experimental. A velocidade linear e a velocidade diagonal da batida dianteira também aumentaram em 11,00% e 6,57%, respectivamente. Sendo que o número de bolas efetivas e de bolas precisas no recuo aumentaram em 5,87% e 5,58%. Conclusão: O treinamento de CrossFit pode melhorar a resistência esportiva dos tenistas, desempenhando um papel positivo na melhora do nível dos jogadores. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução anos praticantes Objetivo Métodos 5 profissionais experimental Resultados intervenção 2 7112 71 12 71,1 6004 60 04 60,0 1100 11 00 11,00 657 6 57 6,57% respectivamente 587 87 5,87 558 58 5,58% Conclusão jogadores II tratamento 711 7 1 71, 600 0 60, 110 11,0 65 6,57 8 5,8 55 5,58 11, 6,5 5, 5,5 6,

ABSTRACT Introduction: The competitive level of tennis has increased in recent years, challenging researchers to promote a higher level of endurance and performance of its practitioners. Objective: Analyze the impacts of CrossFit training on the performance of tennis athletes. Methods: This work conducted a four-week experiment with 50 professional tennis players, introducing a protocol based on CrossFit training to the experimental group. Levels of sports endurance and competition performance were statistically analyzed before and after the experiment. It was also analyzed whether CrossFit training could effectively improve tennis players’ sports endurance and performance level. Results: After the intervention, the time required for the tennis players to take off and hit the ball 20 times in three steps after the recoil was reduced from 71.12 seconds before the experiment to 60.04 seconds in the experimental group. The forehand strike’s linear and diagonal speeds increased by 11.00% and 6.57%, respectively. And the number of effective and accurate balls in the recoil increased by 5.87% and 5.58%. Conclusion: CrossFit training can improve tennis players’ sporting endurance, playing a positive role in improving the players’ level. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction years practitioners Objective athletes Methods fourweek four week 5 group Results intervention 2 7112 71 12 71.1 6004 60 04 60.0 strikes strike s 1100 11 00 11.00 657 6 57 6.57% respectively 587 87 5.87 558 58 5.58% Conclusion II outcomes 711 7 1 71. 600 0 60. 110 11.0 65 6.57 8 5.8 55 5.58 11. 6.5 5. 5.5 6.

Abstract Under the influence of the COVID-19, people's awareness of physical health and immunity has increased significantly. Chitooligosaccharide is an oligomer of β-(1, 4)-linked D -glucosamine, furthermore, is one of the most widely studied immunomodulators. Chitooligosaccharide can be prepared from the chitin or chitosan polymers through enzymatically, chemically or physically processes. Chitooligosaccharide and its derivatives have been proven to have a wide range of biological activities including intestinal flora regulation, immunostimulant, anti-tumor, anti-obesity and anti-oxidation effects. This review summarizes the latest research of the preparation methods, biological activities in immunity and safety profiles of Chitooligosaccharide and its derivatives. We recapped the effect mechanisms of Chitooligosaccharide basing on overall immunity. Comparing the effects of Chitooligosaccharide with different molecular weights and degree of aggregation, a reference range for usage has been provided. This may provide a support for the application of Chitooligosaccharide in immune supplements and food. In addition, future research directions are also discussed. COVID19, COVID19 COVID 19, 19 COVID-19 peoples people s significantly β1, β1 β 1, 1 β-(1 4linked linked 4 glucosamine, glucosamine -glucosamine furthermore immunomodulators enzymatically processes regulation immunostimulant antitumor, antitumor anti tumor, tumor anti-tumor antiobesity obesity antioxidation oxidation methods aggregation provided food addition discussed COVID1 COVID-1 β-( COVID- β-

Regular exercise reduces the risk of malignancy and decreases the recurrence of cancer. However, the mechanisms behind this protection remain to be elucidated. Natural killer (NK) cells are lymphocytes of the innate immune system, which play essential roles in immune defense and effectively prevent cancer metastasis. Physical exercise can increase the activity of NK cells. Interleukin-15 (IL-15) is the best-studied cytokine activator of NK cells, and it was shown to have many positive functional effects on NK cells to improve antitumor responses. The aim of this study was to clarify the possible important mechanisms behind endurance exercise-induced changes in NK cell function, which may be highly correlated with IL-15. An animal model was used to study IL-15 expression level, tumor volume, cancer cell apoptosis, and NK cell infiltration after treadmill exercise. Although IL-15 was highly expressed in skeletal muscle, treadmill exercise further elevated IL-15 levels in plasma and muscle (P<0.05). In addition, tumor weight and volume of tumor-bearing mice were decreased (P<0.05), and liver tumor cell apoptosis was increased after 12 weeks of treadmill exercise (P<0.05). NK cell infiltration was upregulated in tumors from treadmill exercise mice, and the level of interferon-gamma (IFN-γ) and IL-15 were higher than in sedentary mice (P<0.05). The study indicated that regular endurance training can reduce cancer risk, which was related to increased IL-15 expression, activation of the immune killing effect of NK cells, and promotion of tumor cell apoptosis, which can ultimately control tumor growth.

Abstract Objective To compare the clinical features, risk factors, distribution of Benign Paroxysmal Positional Vertigo (BPPV) subtypes, and effectiveness of canalith repositioning between geriatric and non-geriatric patients with BPPV. Methods A total of 400 patients with BPPV were enrolled. Canalith repositioning was performed according to the semicircular canals involved. Patients were divided by age into a geriatric group (≥60 years) and a non-geriatric group (20-59 years). Clinical characteristics, potential age-related risk factors, distribution of subtypes, and effectiveness of canalith repositioning were compared between the groups. Results Female sex was significantly more common in all age groups, with a peak female-to-male ratio of 5.1:1 in the group aged 50-59 years. There was a higher proportion of men in the geriatric group. A history of disease associated with atherosclerosis was significantly more common in the geriatric group (p< 0.05). Migraine was significantly more common in the non-geriatric group (p= 0.018), as was posterior canal BPPV. The horizontal canal BPPV (especially horizontal canal BPPV-cupulolithiasis), and multicanal BPPV subtypes were more common in the geriatric group, whereas anterior canal BPPV was more common in the non-geriatric group. Two canalith repositioning sessions were effective in 58.0% of the geriatric cases and in 72.6% of the non-geriatric cases (p= 0.002). There was a tendency for the effectiveness of canalith repositioning to decrease with increasing age. Conclusion BPPV was more common in women. However, the proportion of men with BPPV increased with age. Elderly patients often had a history of diseases associated with atherosclerosis (i.e., hypertension, diabetes, and hyperlipidemia). The horizontal canal BPPV (particularly horizontal canal BPPV-cupulolithiasis) and multicanal BPPV subtypes were more common and the anterior canal BPPV subtype was less common in elderly patients. The effectiveness of canalith repositioning may decrease with age. Therefore, older patients should receive more comprehensive medical treatment. Level of evidence: 4.

Abstract Objectives The differentiation between Vestibular Migraine (VM) and Meniere's Disease (MD) is difficult because of overlapping symptoms. The study aimed to compare the clinical characteristics and vestibular function test results between VM and MD patients. Methods Seventy-one patients with definite VM and 31 patients with definite unilateral MD were included. All patients received Caloric Test (CT), Video Head Impulse Test (vHIT) and Vestibular Evoked Myogenic Potential (VEMP) test within 7 days after visiting the hospital. Results of these tests were compared between groups. Results Most VM patients (64.0%) experienced spontaneous internal vertigo, while most MD patients (66.7%) experienced spontaneous external vertigo. MD patients had more severe vestibular symptoms and autonomic responses compared to VM patients during attacks (p= 0.03, p= 0.00, respectively). The nystagmus intensity of CT-induced was greater in VM patients than in MD patients (p= 0.003). More VM patients had CT intolerance and Central Positional Nystagmus (CPN) compared to MD patients (p= 0.002, p = 0.006, respectively). More MD patients had CT(+) and vHIT saccades wave compared to VM patients (p< 0.001, p= 0.002, respectively). The non-elicitation rate of cervical VEMP was higher, and the ocular VEMP amplitudes were lower in MD patients than in VM patients (p = 0.002, p= 0.018). Conclusions Vestibular symptoms during attacks combined with the results of vestibular function tests may be used to differentiate between VM and MD. The diverse nature of vestibular symptoms (especially internal vertigo), history of motion sickness and CT intolerance may provide clues to the diagnosis of VM, whereas spontaneous external vertigo, CT(+) with vHIT(-), and the presence of saccades may provide clues to the diagnosis of MD. Level of evidence: 4.

Abstract Allium mongolicum Regel is a desert plant that shows great resistance to wind erosion, drought, and low temperatures and is widely distributed in the desert lands of China. Understanding the molecular mechanisms underlying its drought resistance is essential for uncovering drought resistance genes and improving its beneficial traits. Here, a de novo RNA-Seq assembly analysis was conducted using the roots of 1-month-old seedlings under drought stress. Using pairwise comparisons of untreated plants (CK), 48-hour drought plants (G48h), and 96-hour drought plants (G96h), in total, 2,211 differentially expressed unigenes (DEGs) were obtained. Furthermore, using functional annotation, these DEGs were mainly involved in the plasma membrane, photosynthesis, and secondary carbohydrate metabolism. Moreover, genes involved in the ABA-mediated signaling pathway and secondary metabolism were upregulated in the roots. These results suggest that desert plants may use signaling and secondary metabolic pathways as adaptive responses to drought stress. Collectively, this work could help elucidate the molecular mechanisms underlying the ability of A. mongolicum Regel to respond to drought stress and aid in the selection of novel drought tolerance genes.

Resumen Allium mongolicum Regel es un tipo de planta desértica que muestra una gran resistencia a la erosión eólica, la sequía y las bajas temperaturas; se distribuye ampliamente en las tierras desérticas de China. Comprender los mecanismos moleculares subyacentes a la resistencia a la sequía de A. mongolicum Regel es esencial para descubrir genes resistentes a la sequía y mejorar este rasgo beneficioso en otras plantas. Aquí, el análisis de ensamblaje de ARN-Seq de novo se llevó a cabo utilizando las raíces de plántulas de 1 mes de edad en respuesta al estrés por sequía. Utilizando comparaciones por pares de plantas no tratadas (CK), plantas de 48 horas (G48h) y plantas de 96 horas (G96h), se obtuvieron un total de 2.211 unigenes (DEGs) expresados diferencialmente. Mientras tanto, usando la anotación funcional, estos DEGs fueron implicados principalmente en la membrana de plasma, la fotosíntesis, y el metabolismo de carbohidrato secundario. Por otra parte, los genes implicados en el camino ABA-mediado de la señalización y en metabolismo secundario para arriba fueron regulados para arriba en raíces. Estos resultados sugieren que las plantas del desierto pueden utilizar la señalización y las vías metabólicas secundarias como respuestas adaptativas al estrés por sequía. Colectivamente, este trabajo ayudará a comprender los mecanismos moleculares subyacentes a la capacidad de A. mongolicum Regel para responder al estrés por sequía y ayudar en la selección de nuevos genes de tolerancia a la sequía.

Abstract To determine whether ozone can regulate fruit ripening and delay fruit aging, Kiwifruit (Actinidia deliciosa 'Guichang') was fumigated with different ozone concentrations (100 μL/L, 200 μL/L, 300 μL/L) for 3 h, stored at 1 °C and 85 ± 5% RH for three months, and then matured at 20 °C and 85% RH for 12 days. Compared with controls, the optimal concentration of ozone delayed kiwifruit ripening during cold storage by delaying the respiratory burst that precedes fruit decay. Consequently, fruit firmness, nutrient content, and enzyme activity remained stable; the expression of AdACS1 and AdACO1 as well as the activity of ACC synthetase (ACS) and ACC oxidase (ACO) was downregulated, whereby ACC and MACC were accumulated. In addition, ozone-treated fruit showed significantly reduced infection with grey mold. The effect of ozone on kiwifruit at 200 μL/L was the best suited for delaying fruit senescence, maintaining high quality during storage and fruit ripening compared with other fruits. Overall, our study indicated that ozone may play a major residual role in the physiological process of fruit ripening; furthermore, ozone can affect ethylene biosynthesis and fruit changes associated with endogenous ethylene production.

Abstract Pitaya fruits have high senescence rates throughout their postharvest storage period. Additionally, studies have confirmed that melatonin plays a regulatory role in plant senescence. However, the involvement of melatonin in the postharvest senescence of fruits remains unclear. In this study, two cultivars of pitaya fruit, ‘Zihonglong’ and ‘Jinghonglong’, were treated with melatonin and then evaluated for characteristics of senescence while in storage for 10 days. The results showed that melatonin treatment delayed fruit senescence in both pitaya cultivars, as indicated by the inhibition of weight loss, decay incidence, relative membrane permeability, and malondialdehyde (MDA) content, as well as the maintenance of the total soluble solids and ascorbic acid contents and the reduced respiration intensity. In addition, melatonin treatment reduced the O2·- production rates, H2O2 contents, and lipoxygenase activities but enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) in both pitaya cultivars. These results indicate that melatonin may contribute to delaying senescence in pitaya fruits. This study shows the potential of the use of melatonin in the postharvest storage of pitaya fruits, as well as other horticultural fruits.

Abstract To explore the critical care treatment and clinical outcome of patients with severe aneurysmal subarachnoid hemorrhage (SaSAH), and to provide clinical evidence for the treatment of patients with SaSAH. 160 patients with SaSAH of Hunt and Hess (H&H) grade III or above in the neurosurgical critical care unit at ourospital between June 2016 and June 2018 were enrolled in this study. Patients were treated with brain protective bundle based on step-wised protocol under multimodal monitoring and followed up for 3 months. The incidence of various clinical complications and the clinical prognosis after discharge were statistically analyzed. Furthermore, it carried on the correlation analysis to the risk factors and the prognosis. In 160 patients with SaSAH, 127 patients had responsible aneurysms located in the anterior circulation, and the other 33 patients were located in the posterior circulation. 70 patients (43.8%) underwent transcranial clipping, 81 patients (50.6%) underwent endovascular embolization and 9 patients (5.6%) did not intervene. Patients with IV and V grades of Glasgow Outcome Scale (GOS) were considered good recovery, and those with grade I, II and III were poor prognosis. The 30-day good prognosis rate was 41.3% (66/160) and the poor prognosis rate was 58.7% (94/160). Multivariate logistic regression analysis showed that the Hunt & Hess classification correlated well with prognosis (OR: 4.367,95% CI, 2.254-8.460, P < 0.001). The clinical prognosis of patients with SaSAH is still poor. In particular, under the critical care of neurosurgery, the application of brain protective bundle based on step-wised protocol under multimodal monitoring might improve the clinical prognosis of patients with SaSAH.

Abstract To study the long-term clinical efficacy of protrusion of lumbar intervertebral disc with percutaneous laser disc decompression (PLDD). A statistics was conducted on 108 patients who had been treated with percutaneous laser disc decompression, the intervertebral height of related lesion spaces of patients in different time points after treatment and the intervertebral height ratio was calculated respectively; moreover, a statistics was conducted on VAS evaluation of patients in different time points, and the long-term efficacy after treatment with percutaneous laser disc decompression was evaluated with statistical method. The patients with follow-up period after operation respectively up to 5, 8 and 10 years and those in 3 years after operation were analyzed statistically in intervertebral height ratio and VAS scoring, and the difference was without significant statistical significance. Single-factor was analyzed to the factors likely to influence the effect of operation to get the authenticity of influence of age, disease duration and disease stage on the postoperative efficacy. Percutaneous laser disc decompression was featured by safety, efficacy and fewer complications in treatment of protrusion of lumbar intervertebral disc, and also it had no obvious influence on the spinal stability, and long-term postoperative efficacy was stable.