Abstract Background Cutaneous squamous cell carcinoma (CSCC) is one of the most common types of skin cancer worldwide. Therefore, the identification of biomarkers associated with CSCC progression could aid in the early detection of high-risk squamous cell carcinoma and the development of novel therapeutic strategies. Objective This study aimed to investigate the expression patterns of silent mating type Information Regulation 2 homolog 6 (SIRT6) in CSCC and its clinical significance. Methods The protein expression level of SIRT6 in tissues was detected by immunohistochemistry, and the correlation between SIRT6 expression and clinicopathological parameters in CSCC patients was analyzed. The relative expression of SIRT6 in CSCC cell lineage and tissue specimens was determined by western blotting and PCR. The effect of SIRT6 silencing on cell proliferation was evaluated using cell counting kit 8. Wound healing, transwell method, and flow cytometry were used to investigate the migration, invasion, and cell cycle distribution/apoptosis of CSCC cells after SIRT6 silencing, respectively. Western blot was used to detect the expression of EMT (Epithelial-Mesenchymal Transition), cycle, apoptosis, and other related proteins. Results The high expression of SIRT6 was correlated with the location of cancer tissue and Broder staging in CSCC patients. Knockdown of SIRT6 inhibited the proliferation, migration, invasion and EMT of CSCC cells, and promoted their apoptosis, with cells blocked in G1 phase. Study limitations No animal experiments were conducted to further verify the results. Conclusion Decreased expression of SIRT6 can inhibit the occurrence and development of CSCC. (CSCC worldwide Therefore highrisk risk strategies SIRT (SIRT6 significance immunohistochemistry analyzed PCR 8 healing method migration distributionapoptosis distribution apoptosis respectively EpithelialMesenchymal Epithelial Mesenchymal Transition, Transition , Transition) proteins G phase results (SIRT

Abstract Hanyou 3015 is a new Indica hybrid of water-saving and drought-resistance rice (WDR), combining rapid maturation and high yield potential. Whole genome sequencing results show that Hanyou 3015 carries many superior alleles that could explain its drought tolerance, high water use efficiency, rapid maturation, and wide adaptability. 301 watersaving saving droughtresistance resistance WDR, WDR , (WDR) potential tolerance efficiency adaptability 30 (WDR 3

ABSTRACT Purpose: Visual impairment and blindness caused by cataracts are major public health problems. Several factors are associated with an increased risk of age-related cataracts, such as age, smoking, alcohol consumption, and ultraviolet radiation. This meta-analysis aimed to assess the association between body mass index and age-related cataracts. Methods: Studies on weight and age-related cataracts published from January 2011 to July 2020 were reviewed by searching PubMed, Medline, and Web of Science databases. The random-effects and fixed-effects models were used for the meta-analysis, and the results were reported as odd ratios. Results: A total of nine studies were included in the meta-analysis. No correlation was found between underweight and nuclear cataracts (OR=1.31, 95% CI [-0.50 to 3.12], p=0.156). The results of the random-effects model showed that overweight was significantly associated with age-related cataracts and reduced the risk of age-related cataracts (OR=0.91, 95% CI [0.80-1.02], p<0.0001; I2=62.3%, p<0.0001). Significant correlations were found between overweight and cortical, nuclear, and posterior subcapsular cataracts (OR=0.95, 95% CI [0.66-1.24], p<0.0001; OR=0.92, 95% CI (0.76-1.08), p<0.0001; OR=0.87, 95% CI [0.38-1.02], p<0.0001). Significant correlations were found between obesity and cortical, nuclear, and posterior subcapsular cataracts (OR=1.00, 95% CI [0.82-1.17], p<0.0001; OR=1.07, 95% CI [0.92-1.22], p<0.0001; OR=1.14, 95% CI [0.91-1.37], p<0.0001). Conclusion: This finding suggested a significant correlation between body mass index and age-related cataracts, with overweight and obesity reducing or increasing the risk of age-related cataracts, respectively. Purpose problems agerelated age related smoking consumption radiation metaanalysis meta analysis Methods 201 202 PubMed Medline databases randomeffects random effects fixedeffects fixed metaanalysis, analysis, ratios Results metaanalysis. analysis. OR=1.31, OR131 OR 1 31 (OR=1.31 95 0.50 050 0 50 [-0.5 3.12, 312 3.12 , 3 12 3.12] p=0.156. p0156 p p=0.156 . 156 p=0.156) OR=0.91, OR091 91 (OR=0.91 0.801.02, 080102 0.80 1.02 80 02 [0.80-1.02] p<0.0001 p00001 0001 I2623 I I2 62 I2=62.3% p<0.0001. p<0.0001) cortical OR=0.95, OR095 (OR=0.95 0.661.24, 066124 0.66 1.24 66 24 [0.66-1.24] OR092 92 OR=0.92 0.761.08, 076108 0.76 1.08 76 08 (0.76-1.08) OR087 87 OR=0.87 0.381.02, 038102 0.38 38 [0.38-1.02] OR=1.00, OR100 00 (OR=1.00 0.821.17, 082117 0.82 1.17 82 17 [0.82-1.17] OR107 07 OR=1.07 0.921.22, 092122 0.92 1.22 22 [0.92-1.22] OR114 14 OR=1.14 0.911.37, 091137 0.91 1.37 37 [0.91-1.37] Conclusion respectively 20 OR=1.31 OR13 (OR=1.3 9 0.5 05 5 [-0. 3.1 p015 p=0.15 15 OR=0.91 OR09 (OR=0.9 801 0.801.02 08010 080 0.8 102 1.0 8 [0.80-1.02 p<0.000 p0000 000 I262 6 I2=62.3 OR=0.95 661 0.661.24 06612 066 0.6 124 1.2 2 [0.66-1.24 OR=0.9 761 0.761.08 07610 076 0.7 108 7 (0.76-1.08 OR08 OR=0.8 381 0.381.02 03810 038 0.3 [0.38-1.02 OR=1.00 OR10 (OR=1.0 821 0.821.17 08211 082 117 1.1 [0.82-1.17 OR=1.0 921 0.921.22 09212 092 0.9 122 [0.92-1.22 OR11 OR=1.1 911 0.911.37 09113 091 137 1.3 [0.91-1.37 OR=1.3 OR1 (OR=1. 0. [-0 3. p01 p=0.1 OR0 (OR=0. 0.801.0 0801 10 1. [0.80-1.0 p<0.00 p000 I26 I2=62. 0.661.2 0661 06 [0.66-1.2 OR=0. 0.761.0 0761 (0.76-1.0 0.381.0 0381 03 [0.38-1.0 0.821.1 0821 11 [0.82-1.1 OR=1. 0.921.2 0921 09 [0.92-1.2 0.911.3 0911 13 [0.91-1.3 (OR=1 [- p0 p=0. (OR=0 0.801. [0.80-1. p<0.0 p00 I2=62 0.661. [0.66-1. OR=0 0.761. (0.76-1. 0.381. [0.38-1. 0.821. [0.82-1. OR=1 0.921. [0.92-1. 0.911. [0.91-1. (OR= [ p=0 0.801 [0.80-1 p<0. I2=6 0.661 [0.66-1 OR= 0.761 (0.76-1 0.381 [0.38-1 0.821 [0.82-1 0.921 [0.92-1 0.911 [0.91-1 (OR p= [0.80- p<0 I2= [0.66- (0.76- [0.38- [0.82- [0.92- [0.91- [0.80 p< [0.66 (0.76 [0.38 [0.82 [0.92 [0.91 [0.8 [0.6 (0.7 [0.3 [0.9 [0. (0. [0 (0 (

RESUMO Objetivo: A deficiência visual e a cegueira causadas pela catarata são um grande problema de saúde pública. Há vários fatores associados a um risco maior de catarata relacionada à idade na população mundial, tais como idade, tabagismo, consumo de álcool e radiação ultravioleta. Esta meta-análise foi realizada para avaliar a associação entre o índice de massa corporal e a catarata relacionada à idade. Métodos: Foi revisada a literatura sobre catarata relacionada a peso e idade publicada de janeiro de 2011 a julho de 2020, através de buscas nos bancos de dados PubMed, Medline e Web of Science. Na meta-análise, foram utilizados modelos de efeito aleatórios e de efeitos fixos e os resultados foram apresentados como razões de chances (OR). Resultados: Um total de 9 estudos foi incluído na meta-análise. Não houve correlação entre ausência de sobrepeso e cataratas nucleares (OR=1,31, IC 95%: -0,50-3,12, p=0,156). Os resultados do modelo de efeitos aleatórios mostraram que o excesso de peso estava significativamente associado a uma redução do risco de catarata relacionada à idade (OR=0,91, IC 95%: 0,80-1,02, p<0,0001, I2=62,3%, p<0,0001). Houve correlações significativas entre o excesso de peso e cataratas corticais (OR=0,95, IC 95%: 0,66-1,24, p<0,0001), nucleares (OR=0,92, IC 95%: 0,76-1,08, p<0,0001) e subcapsulares posteriores (OR=0,87, IC 95%: 0,38-1,02, p<0,0001) relacionadas à idade. Houve correlações significativas entre obesidade e cataratas corticais (OR=1,00, IC 95%: 0,82-1,17, p<0,0001), nucleares (OR=1,07, IC 95%: 0,92-1,22, p<0,0001) e subcapsulares posteriores (OR=1,14, IC 95%: 0,91-1,37, p<0,0001) relacionadas à idade. Conclusão: Estes achados sugeriram uma correlação significativa entre o índice de massa corporal e a catarata relacionada à idade, com o excesso de peso e a obesidade reduzindo e aumentando o risco de catarata relacionada à idade, respectivamente. Objetivo pública mundial tabagismo ultravioleta metaanálise meta análise Métodos 201 2020 PubMed Science metaanálise, análise, OR. OR . (OR) Resultados metaanálise. análise. OR=1,31, OR131 1 31 (OR=1,31 95% 95 0,503,12, 050312 0,50 3,12, 0 50 3 12 -0,50-3,12 p=0,156. p0156 p p=0,156 156 p=0,156) OR=0,91, OR091 91 (OR=0,91 0,801,02, 080102 0,80 1,02, 80 02 0,80-1,02 p00001 0001 p<0,0001 I2623 I I2 62 I2=62,3% p<0,0001. OR=0,95, OR095 (OR=0,95 0,661,24, 066124 0,66 1,24, 66 24 0,66-1,24 , OR=0,92, OR092 92 (OR=0,92 0,761,08, 076108 0,76 1,08, 76 08 0,76-1,08 OR=0,87, OR087 87 (OR=0,87 0,381,02, 038102 0,38 38 0,38-1,02 OR=1,00, OR100 00 (OR=1,00 0,821,17, 082117 0,82 1,17, 82 17 0,82-1,17 OR=1,07, OR107 07 (OR=1,07 0,921,22, 092122 0,92 1,22, 22 0,92-1,22 OR=1,14, OR114 14 (OR=1,14 0,911,37, 091137 0,91 1,37, 37 0,91-1,37 Conclusão respectivamente 20 202 (OR OR=1,31 OR13 (OR=1,3 503 0,503,12 05031 050 0,5 312 3,12 5 -0,50-3,1 p015 p=0,15 15 OR=0,91 OR09 (OR=0,9 801 0,801,02 08010 080 0,8 102 1,02 8 0,80-1,0 p0000 000 p<0,000 I262 6 I2=62,3 OR=0,95 661 0,661,24 06612 066 0,6 124 1,24 2 0,66-1,2 OR=0,92 761 0,761,08 07610 076 0,7 108 1,08 7 0,76-1,0 OR=0,87 OR08 (OR=0,8 381 0,381,02 03810 038 0,3 0,38-1,0 OR=1,00 OR10 (OR=1,0 821 0,821,17 08211 082 117 1,17 0,82-1,1 OR=1,07 921 0,921,22 09212 092 0,9 122 1,22 0,92-1,2 OR=1,14 OR11 (OR=1,1 911 0,911,37 09113 091 137 1,37 0,91-1,3 OR=1,3 OR1 (OR=1, 0,503,1 0503 05 0, 3,1 -0,50-3, p01 p=0,1 OR=0,9 OR0 (OR=0, 0,801,0 0801 10 1,0 0,80-1, p000 p<0,00 I26 I2=62, 0,661,2 0661 06 1,2 0,66-1, 0,761,0 0761 0,76-1, OR=0,8 0,381,0 0381 03 0,38-1, OR=1,0 0,821,1 0821 11 1,1 0,82-1, 0,921,2 0921 09 0,92-1, OR=1,1 0,911,3 0911 13 1,3 0,91-1, OR=1, (OR=1 0,503, 3, -0,50-3 p0 p=0, OR=0, (OR=0 0,801, 1, 0,80-1 p00 p<0,0 I2=62 0,661, 0,66-1 0,761, 0,76-1 0,381, 0,38-1 0,821, 0,82-1 0,921, 0,92-1 0,911, 0,91-1 OR=1 (OR= 0,503 -0,50- p=0 OR=0 0,801 0,80- p<0, I2=6 0,661 0,66- 0,761 0,76- 0,381 0,38- 0,821 0,82- 0,921 0,92- 0,911 0,91- OR= -0,50 p= p<0 I2= -0,5 p< -0, -0 -

Abstract Objective To explore the relationship between Growth Hormone Insulin-like Growth Factors (GH-IGFs) and growth retardation in children with bronchial asthma. Methods 112 children with bronchial asthma and 50 healthy children were studied. Serum GH, IGF-1, and Insulin-like Growth Factor Binding Protein 3 (IGFBP3) were assessed by ELISA. GH-IGFs-related parameters were compared, and the correlation between the parameters and bronchial asthma severity was analyzed. The bronchial asthma group was divided into the growth retardation group and non-growth retardation group to analyze the diagnostic value of GH-IGFs in growth retardation and the relationship between GH-IGFs and growth retardation. Results GH, IGF-1, and IGFBP3 in the bronchial asthma group were lower. GH, IGF-1, and IGFBP3 levels were decreased with the severity of bronchial asthma. GH, IGF-1, and IGFBP3 in the growth retardation group were lower than those in the non-growth retardation group. The AUC of GH-IGFs combined detection was higher than that of GH and IGFBP3 alone detection. GH < 9.27 μg/L and IGF-1 < 179.53 mmoL/L were risk factors for growth retardation in patients with bronchial asthma. Conclusion GH-IGFs-related parameters have diagnostic value for growth retardation in children, and decreased levels of GH and IGF-1 are risk factors for growth retardation in children. Insulinlike Insulin like GHIGFs IGFs (GH-IGFs 11 5 studied IGF1, IGF1 IGF 1, 1 IGFBP (IGFBP3 ELISA GHIGFsrelated related compared analyzed nongrowth non 927 9 27 9.2 μgL μg L IGF- 17953 179 53 179.5 mmoLL mmoL (IGFBP 92 2 9. 1795 17 179.

Resumo Antecedentes O diagnóstico precoce da doença de Alzheimer (DA) e do comprometimento cognitivo leve (CCL) continua sendo um desafio significativo na neurologia, com métodos convencionais frequentemente limitados pela subjetividade e variabilidade na interpretação. A integração da aprendizagem profunda com a inteligência artificial (IA) na análise de imagens de ressonância magnética surge como uma abordagem transformadora, oferecendo o potencial para insights diagnósticos imparciais e altamente precisos. Objetivo Uma metanálise foi projetada para analisar a precisão diagnóstica do aprendizado profundo de imagens de ressonância magnética em modelos de DA e CCL. Métodos Uma metanálise foi realizada nos bancos de dados das bibliotecas PubMed, Embase e Cochrane seguindo as diretrizes Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), com foco na precisão diagnóstica do aprendizado profundo. Posteriormente, a qualidade metodológica foi avaliada por meio do checklist QUADAS-2. Medidas diagnósticas, incluindo sensibilidade, especificidade, razões de verossimilhança, razão de chances diagnósticas e área sob a curva característica de operação do receptor (area under the receiver operating characteristic curve [AUROC]) foram analisadas, juntamente com análises de subgrupo para ressonância magnética ponderada em T1 e não ponderada em T1. Resultados Um total de 18 estudos elegíveis foram identificados. O coeficiente de correlação de Spearman foi de -0,6506. A metanálise mostrou que a sensibilidade e a especificidade combinadas, a razão de verossimilhança positiva, a razão de verossimilhança negativa e a razão de chances de diagnóstico foram 0,84, 0,86, 6,0, 0,19 e 32, respectivamente. A AUROC foi de 0,92. O ponto quiescente do resumo hierárquico da característica de operação do receptor (hierarchical summary of receiver operating characteristic [HSROC]) foi 3,463. Notavelmente, as imagens de 12 estudos foram adquiridas apenas por ressonância magnética ponderada em T1, e as dos outros 6 foram obtidas apenas por ressonância magnética não ponderada em T1. Conclusão Em geral, a aprendizagem profunda da ressonância magnética para o diagnóstico de DA e CCL mostrou boa sensibilidade e especificidade e contribuiu para melhorar a precisão diagnóstica. (DA (CCL neurologia interpretação IA (IA transformadora precisos PubMed MetaAnalyses Meta Analyses PRISMA, PRISMA , (PRISMA) Posteriormente QUADAS2. QUADAS2 QUADAS 2. 2 QUADAS-2 area [AUROC] analisadas T 1 identificados 0,6506. 06506 0 6506 -0,6506 combinadas positiva 084 84 0,84 086 86 0,86 60 6,0 019 19 0,1 32 respectivamente 092 92 0,92 hierarchical HSROC [HSROC] 3463 3 463 3,463 Notavelmente geral (PRISMA QUADAS- [AUROC 0,6506 0650 650 -0,650 08 8 0,8 6, 01 0, 09 9 0,9 [HSROC 346 46 3,46 0,650 065 65 -0,65 34 4 3,4 0,65 06 -0,6 3, 0,6 -0, -0 -

Abstract Background The early diagnosis of Alzheimer's disease (AD) and mild cognitive impairment (MCI) remains a significant challenge in neurology, with conventional methods often limited by subjectivity and variability in interpretation. Integrating deep learning with artificial intelligence (AI) in magnetic resonance imaging (MRI) analysis emerges as a transformative approach, offering the potential for unbiased, highly accurate diagnostic insights. Objective A meta-analysis was designed to analyze the diagnostic accuracy of deep learning of MRI images on AD and MCI models. Methods A meta-analysis was performed across PubMed, Embase, and Cochrane library databases following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, focusing on the diagnostic accuracy of deep learning. Subsequently, methodological quality was assessed using the QUADAS-2 checklist. Diagnostic measures, including sensitivity, specificity, likelihood ratios, diagnostic odds ratio, and area under the receiver operating characteristic curve (AUROC) were analyzed, alongside subgroup analyses for T1-weighted and non-T1-weighted MRI. Results A total of 18 eligible studies were identified. The Spearman correlation coefficient was -0.6506. Meta-analysis showed that the combined sensitivity and specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 0.84, 0.86, 6.0, 0.19, and 32, respectively. The AUROC was 0.92. The quiescent point of hierarchical summary of receiver operating characteristic (HSROC) was 3.463. Notably, the images of 12 studies were acquired by T1-weighted MRI alone, and those of the other 6 were gathered by non-T1-weighted MRI alone. Conclusion Overall, deep learning of MRI for the diagnosis of AD and MCI showed good sensitivity and specificity and contributed to improving diagnostic accuracy. Alzheimers Alzheimer s (AD (MCI neurology interpretation AI (AI (MRI approach unbiased insights metaanalysis meta models PubMed Embase MetaAnalyses Meta Analyses PRISMA (PRISMA guidelines Subsequently QUADAS2 QUADAS 2 QUADAS- checklist measures ratios (AUROC analyzed T1weighted Tweighted T1 weighted T nonT1weighted nonTweighted non 1 identified 0.6506. 06506 0 6506 -0.6506 Metaanalysis 084 84 0.84 086 86 0.86 60 6.0 019 19 0.19 32 respectively 092 92 0.92 HSROC (HSROC 3463 3 463 3.463 Notably alone Overall nonT 0.6506 0650 650 -0.650 08 8 0.8 6. 01 0.1 09 9 0.9 346 46 3.46 0.650 065 65 -0.65 0. 34 4 3.4 0.65 06 -0.6 3. 0.6 -0. -0 -

ABSTRACT Objective: The optimal target for blood glucose concentration in critically ill patients is unclear. We will perform a systematic review and meta-analysis with aggregated and individual patient data from randomized controlled trials, comparing intensive glucose control with liberal glucose control in critically ill adults. Data sources: MEDLINE®, Embase, the Cochrane Central Register of Clinical Trials, and clinical trials registries (World Health Organization, clinical trials.gov). The authors of eligible trials will be invited to provide individual patient data. Published trial-level data from eligible trials that are not at high risk of bias will be included in an aggregated data meta-analysis if individual patient data are not available. Methods: Inclusion criteria: randomized controlled trials that recruited adult patients, targeting a blood glucose of ≤ 120mg/dL (≤ 6.6mmol/L) compared to a higher blood glucose concentration target using intravenous insulin in both groups. Excluded studies: those with an upper limit blood glucose target in the intervention group of > 120mg/dL (> 6.6mmol/L), or where intensive glucose control was only performed in the intraoperative period, and those where loss to follow-up exceeded 10% by hospital discharge. Primary endpoint: In-hospital mortality during index hospital admission. Secondary endpoints: mortality and survival at other timepoints, duration of invasive mechanical ventilation, vasoactive agents, and renal replacement therapy. A random effect Bayesian meta-analysis and hierarchical Bayesian models for individual patient data will be used. Discussion: This systematic review with aggregate and individual patient data will address the clinical question, ‘what is the best blood glucose target for critically ill patients overall?’ Protocol version 0.4 - 06/26/2023 PROSPERO registration: CRD42021278869 Objective unclear metaanalysis meta analysis adults sources MEDLINE MEDLINE® Embase Trials World Organization trials.gov. trialsgov trials.gov . gov trials.gov) triallevel trial level available Methods criteria 120mgdL mgdL 120mg dL mg ( 6.6mmol/L 66mmolL mmolL 6 6mmol L mmol groups studies 6.6mmol/L, , period followup follow up 10 discharge endpoint Inhospital In admission endpoints timepoints ventilation agents therapy used Discussion question what overall overall? 04 0 4 0. 06262023 06 26 2023 06/26/202 registration CRD CRD4202127886 1 0626202 2 202 06/26/20 CRD420212788 062620 20 06/26/2 CRD42021278 06262 06/26/ CRD4202127 0626 06/26 CRD420212 062 06/2 CRD42021 06/ CRD4202 CRD420 CRD42 CRD4

RESUMO Objetivo: Não está claro qual é a meta ideal de concentração de glicose no sangue em pacientes em estado grave. Realizaremos uma revisão sistemática e uma metanálise com dados agregados e de pacientes individuais de estudos controlados e randomizados, comparando o controle intensivo da glicose com o controle liberal da glicose em adultos em estado grave. Fontes de dados: MEDLINE®, Embase, Cochrane Central Register of Clinical Trials e registros de ensaios clínicos (Organização Mundial da Saúde, clinical trials.gov). Os autores dos estudos qualificados serão convidados a fornecer dados individuais de pacientes. Os dados publicados em nível de ensaio qualificado que não apresentem alto risco de viés serão incluídos em uma metanálise de dados agregados se os dados individuais de pacientes não estiverem disponíveis. Métodos: Critérios de inclusão: ensaios clínicos controlados e randomizados que recrutaram pacientes adultos, com meta de glicemia ≤ 120mg/dL (≤ 6,6mmol/L) comparada a uma meta de concentração de glicemia mais alta com insulina intravenosa em ambos os grupos. Estudos excluídos: aqueles com meta de glicemia no limite superior no grupo de intervenção > 120mg/dL (> 6,6mmol/L), ou em que o controle intensivo de glicose foi realizado apenas no período intraoperatório, e aqueles em que a perda de seguimento excedeu 10% até a alta hospitalar. Desfecho primário: Mortalidade intra-hospitalar durante a admissão hospitalar. Desfechos secundários: Mortalidade e sobrevida em outros momentos, duração da ventilação mecânica invasiva, agentes vasoativos e terapia de substituição renal. Utilizaremos metanálise bayesiana de efeito randômico e modelos bayesianos hierárquicos para dados individuais de pacientes. Discussão: Essa revisão sistemática com dados agregados e de pacientes individuais abordará a questão clínica: Qual é a melhor meta de glicose no sangue de pacientes graves em geral? Protocolo versão 0.4 - 26/06/2023 Registro PROSPERO: CRD42021278869 Objetivo grave MEDLINE MEDLINE® Embase Organização Saúde trials.gov. trialsgov trials.gov . trials gov trials.gov) disponíveis Métodos inclusão 120mgdL mgdL 120mg dL mg ( 6,6mmol/L 66mmolL mmolL 6 6mmol L mmol grupos excluídos 6,6mmol/L, , intraoperatório 10 hospitalar primário intrahospitalar intra secundários momentos invasiva renal Discussão clínica geral 04 0 4 0. 26062023 26 06 2023 26/06/202 PROSPERO CRD CRD4202127886 1 2606202 2 202 26/06/20 CRD420212788 260620 20 26/06/2 CRD42021278 26062 26/06/ CRD4202127 2606 26/06 CRD420212 260 26/0 CRD42021 26/ CRD4202 CRD420 CRD42 CRD4

Abstract Objective To explore whether Cyclic Adenosine Monophosphate (cAMP)-Epac1 signaling is activated in 1-Desamino-8-D-arginine-Vasopressin-induced Endolymphatic Hydrops (DDAVP-induced EH) and to provide new insight for further in-depth study of DDAVP-induced EH. Methods Eighteen healthy, red-eyed guinea pigs (36 ears) weighing 200-350 g were randomly divided into three groups: the control group, which received intraperitoneal injection of sterile saline (same volume as that in the other two groups) for 7 consecutive days; the DDAVP-7d group, which received intraperitoneal injection of 10 mg/mL/kg DDAVP for 7 consecutive days; and the DDAVP-14d group, which received intraperitoneal injection of 10 μg/mL/kg DDAVP for 14 consecutive days. After successful modeling, all animals were sacrificed, and cochlea tissues were collected to detect the mRNA and protein expression of the exchange protein directly activated by cAMP-1 and 2 (Epac1, Epac2), and Repressor Activator Protein-1 (Rap1) by Reverse Transcription (RT)-PCR and western blotting, respectively. Results Compared to the control group, the relative mRNA expression of Epac1, Epac2, Rap1A, and Rap1B in the cochlea tissue of the DDAVP-7d group was significantly higher (p< 0.05), while no significant difference in Rap1 GTPase activating protein (Rap1gap) mRNA expression was found between the two groups. The relative mRNA expression of Epac1, Rap1A, Rap1B, and Rap1gap in the cochlea tissue of the DDAVP-14d group was significantly higher than that of the control group (p< 0.05), while no significant difference in Epac2 mRNA expression was found between the DDAVP-14d and control groups. Comparison between the DDAVP-14d and DDAVP-7d groups showed that the DDAVP-14d group had significantly lower Epac2 and Rap1A (p< 0.05) and higher Rap1gap (p < 0.05) mRNA expression in the cochlea tissue than that of the DDAVP-7d group, while no significant differences in Epac1 and Rap1B mRNA expression were found between the two groups. Western blotting showed that Epac1 protein expression in the cochlea tissue was the highest in the DDAVP-14d group, followed by that in the DDAVP-7d group, and was the lowest in the control group, showing significant differences between groups (p< 0.05); Rap1 protein expression in the cochlea tissue was the highest in the DDAVP-7d group, followed by the DDAVP-14d group, and was the lowest in the control group, showing significant differences between groups (p< 0.05); no significant differences in Epac2 protein expression in the cochlea tissue were found among the three groups. Conclusion DDAVP upregulated Epac1 protein expression in the guinea pig cochlea, leading to activation of the inner ear cAMP-Epac1 signaling pathway. This may be an important mechanism by which DDAVP regulates endolymphatic metabolism to induce EH and affect inner ear function. Oxford Centre for Evidence-Based Medicine 2011 Levels of Evidence Level 5. cAMPEpac1 cAMPEpac cAMP Epac (cAMP)-Epac 1Desamino8DarginineVasopressininduced DesaminoDarginineVasopressininduced 1 Desamino 8 D arginine Vasopressin induced DDAVPinduced indepth depth healthy redeyed red eyed 36 (3 ears 200350 200 350 200-35 same days DDAVP7d DDAVPd 7d d mgmLkg mg mL kg DDAVP14d 14d μgmLkg μg modeling sacrificed cAMP1 cAMP- (Epac1 , Epac2) Protein1 Protein Protein- Rap (Rap1 RTPCR RT PCR respectively RapA A RapB B p< p 0.05, 005 0.05 0 05 Rapgap gap (Rap1gap cAMP-Epac pathway function EvidenceBased Based 201 5 DarginineVasopressininduced 3 ( 20035 20 35 200-3 (Epac (Rap 00 0.0 2003 200- 0.

Abstract Blumea balsamifera (Sembung) is a very famous ethnic medicinal herbs and functional tea in many countries of Asia. Phytochemical investigations showed that B. balsamifera contains various type chemical components, including volatile oil, flavonoids, terpenoids, phenylpropanoids, polysaccharides, etc. B. balsamifera has many pharmacological activities such as antioxidation, antibiosis, antitumor, liver protection, anti-inflammation, analgesia, and wound healing. This paper reviewed the chemical constituents and pharmacological activities of B. balsamifera for the further research, development, and utilization of B. balsamifera. Sembung (Sembung Asia B components oil flavonoids terpenoids phenylpropanoids polysaccharides etc antioxidation antibiosis antitumor protection antiinflammation, antiinflammation anti inflammation, inflammation anti-inflammation analgesia healing research development

RESUMEN Introducción El entorno del deporte universitario se caracteriza por su alcance, diversidad y personalidad. Es un periodo importante para desarrollar la capacidad física de los alumnos y mejorar su personalidad. Objetivo Comparar los efectos de diferentes métodos de ejercicio sobre el estado de salud de los estudiantes. Métodos 2991 estudiantes universitarios participaron en diferentes actividades deportivas. Estos deportes se llevaron a cabo basándose en el curso de selección (PE), todo ello durante un semestre. El estado de salud física de los alumnos se observó mediante experimentos realizados antes y después de la intervención. Resultados Actividades como el baloncesto y el fútbol mostraron una alta eficacia para mejorar el índice de capacidad vital de los alumnos, el voleibol mejoró expresivamente el rendimiento de los alumnos en salto de longitud, el tenis y el tenis de mesa fueron eficaces para mejorar el índice de fuerza y adherencia de los alumnos, siendo inferiores en otros índices. También las artes marciales se destacaron en la mejora del índice de capacidad vital de los alumnos. Conclusión La mejora de la salud física debería ser un proceso global del desarrollo de la forma física de los estudiantes y las universidades deberían animar activamente a los universitarios a participar en deportes de larga duración para mejorar su salud. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. alcance personalidad 299 deportivas PE, PE , (PE) semestre intervención longitud índices II tratamiento 29 (PE 2

ABSTRACT Introduction The college sports environment is characterized by breadth, diversity, and personality. This is an important period to develop students’ physical ability and improve their personality. Objective Compare the effects of different exercise methods on students’ health status. Methods 2991 college students participated in different sports activities. These sports were conducted based on the selection course (PE), all during one semester. The students’ physical health status was observed through experiments performed before and after the intervention. Results Activities such as basketball and soccer showed high effectiveness in improving students’ vital capacity index, volleyball expressively improved students’ performance in the long jump, tennis and table tennis were effective in improving students’ strength and adherence index, being lower in other indices. Martial arts also stood out in improving the students’ vital capacity index. Conclusion Improving physical health should be an overall process of students’ fitness development, and universities should actively encourage college students to participate in long-term sports to improve their health. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. breadth diversity personality 299 activities PE, PE , (PE) semester intervention index jump indices development longterm term II outcomes 29 (PE 2

RESUMO Introdução O ambiente do esporte universitário é caracterizado pela abrangência, diversidade e personalidade. Este é um período importante para desenvolver a capacidade física dos estudantes e melhorar a sua personalidade. Objetivo Comparar os efeitos de diferentes métodos de exercício sobre o estado de saúde dos estudantes. Métodos 2991 estudantes universitários participaram de diferentes atividades esportivas. Estes esportes foram conduzidos com base no curso de seleção (PE), todos durante um semestre. O estado de saúde física dos estudantes foi observado através de experimentos executados previa e posteriormente à intervenção. Resultados Atividades como basquetebol e futebol demonstraram alta efetividade para melhorar o índice de capacidade vital dos estudantes, voleibol melhorou expressivamente o desempenho dos alunos no salto em distância, o tênis e o tênis de mesa foram efetivos para aprimorar o índice de força e de adesão dos alunos, sendo inferior noutros índices. Também as artes marciais se destacaram ao melhorar o índice de capacidade vital dos alunos. Conclusão O aprimoramento da saúde física deve ser um processo global de desenvolvimento da aptidão física dos estudantes e as universidades devem encorajar ativamente os estudantes universitários a participar de esportes de longo prazo para melhorar sua saúde. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. abrangência personalidade 299 esportivas PE, PE , (PE) semestre intervenção distância índices II tratamento 29 (PE 2

As an important component of solid tumors, mast cells show specific phenotypes in various tumor microenvironments. However, the precise mechanism of mast cell accumulation and the phenotypic features of thyroid cancer (TC) remain largely unknown. Here, we found that mast cells were obviously recruited to tumor tissue by TC-derived stem cell factor (SCF). With tumor progression, mast cell levels increased gradually. In addition, intratumoral mast cells expressed higher levels of the immunosuppressive molecule galectin-9, which effectively suppresses CD8+ T-cell antitumor immunity in vitro. Blocking galectin-9 on tumor-infiltrating mast cells reversed the immunosuppression of CD8+ T cells. In conclusion, our data elucidated novel protumorigenic and immunosuppressive roles of mast cells in TC. In addition, our results indicated that blocking mast cells may impede tumor progression and ameliorate the prognosis of TC patients. tumors microenvironments However (TC unknown Here TCderived derived SCF. SCF . (SCF) gradually addition galectin9, galectin9 galectin 9, 9 CD8 CD Tcell vitro galectin- tumorinfiltrating infiltrating conclusion patients (SCF

ABSTRACT In this paper, based on the analysis of oil sludge composition and environmental hazards, the microwave catalytic pyrolysis of oil sludge for recovering combustible gas and chain hydrocarbon light oil disposal process, is proposed to perform the recycling of oil sludge and reduce the risk of environmental pollution. In order to improve the yield of combustible gas and light oil in oil sludge pyrolysis, the impact of adding four catalysts (nano-CuO, nano-NiO, nano-MgO, and nano-γ-Al2O3) on the heating characteristics of oil sludge and the composition and quality of products during microwave pyrolysis, were experimentally studied and analyzed. The results show that the catalyst can increase the heating rate of sludge and the yield of pyrolysis oil and gas. The increasing order of heating is: nano-CuO > nano-γ-Al2O3 > nano-MgO > nano-NiO > blank group. Compared with the blank group, the content of combustible gas (H2 + CH4 + CO) increases by 18.824wt% (MgO), 4.511wt% (CuO), 9.28wt% (NiO), and 16.164wt% (γ-Al2O3), respectively. It can be deduced that nano-metal oxide catalysts, especially γ-Al2O3, can promote the decomposition of oil sludge, improve the content of low carbon straight chain hydrocarbons and alcohols, and improve the quality of pyrolysis oil products. paper hazards process pollution nanoCuO, nanoCuO nano CuO, CuO (nano-CuO nanoNiO, nanoNiO NiO, NiO nanoMgO, nanoMgO MgO, MgO nanoγAl2O3 nanoγAlO γ Al2O3 Al O analyzed nano-γ-Al2O group H2 H (H CH CO 18824wt wt 18 824wt 18.824wt , (MgO) 4511wt 4 511wt 4.511wt (CuO) 928wt 9 28wt 9.28wt (NiO) 16164wt 16 164wt 16.164wt γAl2O3, γAl2O3 γAlO (γ-Al2O3) respectively nanometal metal Al2O3, γ-Al2O3 alcohols nanoγAl nanoγAl2O AlO Al2O 1 (MgO (CuO (NiO γAl γAl2O (γ-Al2O3 γ-Al2O (γ-Al2O

ABSTRACT Due to its high nutritional value, broccoli (Brassica oleracea var. italica Plenck) is one of the most popular vegetables worldwide. This study assessed 36 phenotypic characteristics of 111 broccoli varieties to understand the phenotypic diversity of new broccoli varieties and improve their breeding speed with advantages and characteristics in China, including 108 new varieties and three varieties of common knowledge. The genetic diversity, the principal component, and the cluster of phenotypic characteristics of broccoli varieties were further investigated. The results showed that the coefficients of variation of 36 characteristics ranged between 11.18 % and 94.99 %, with their diversity index between 0.26 and 1.82. The 111 broccoli varieties were further classified into eight groups, primarily attributed to the differences in phenotypic characteristics, including curd weight, main stem thickness, plant development degree, plant height, and anthocyanin coloration. The cumulative contribution rate of the first five principal components reached 81.186 %, corresponding to 12 representative phenotypic traits. The analysis indicated that the phenotypic characteristics of broccoli were rich in diversity, especially for several characteristics appreciated by the market, such as weight, curd firmness, and anthocyanin coloration. This study revealed the basic information on the genetic diversity of new broccoli varieties in China from 2017 to 2019 and provided potential breeding strategies for broccoli to meet diverse market demands. value Brassica var Plenck worldwide 3 11 10 knowledge component investigated 1118 18 11.1 9499 94 99 94.9 026 0 26 0.2 182 1 82 1.82 groups weight thickness degree height coloration 81186 81 186 81.18 traits firmness 201 demands 11. 949 9 94. 02 2 0. 8 1.8 8118 81.1 20 1. 811 81.

SUMMARY OBJECTIVE: This study aimed to investigate the sex-specific association between hyperuricemia and the risk of hypertension and whether obesity mediates this association. METHODS: This study included 31,395 (47.0% women) adults without hypertension, cardiovascular disease, or cancer at baseline who completed at least one follow-up annual examination between 2009 and 2016. Cox regression models were performed to calculate hazard ratios and 95% confidence intervals. Mediation analysis was conducted to estimate the effect of body mass index on the association between hyperuricemia and hypertension. RESULTS: During a median 2.9-year follow-up, hyperuricemia was significantly associated with a higher risk of hypertension (HR 1.15, 95%CI 1.07-1.24 for all participants; HR 1.12, 95%CI 1.03-1.22 for men; and HR 1.23, 95%CI 1.02-1.48 for women) after adjustment for potential confounders. Additional adjustment for body mass index attenuated this association (HR 1.09, 95%CI 1.08-1.10 for all participants; HR 1.07; 95%CI 0.98-1.16 for men; HR 1.18; 95%CI 0.96-1.44 for women). Mediation analysis showed that BMI partially mediated the relationship between hyperuricemia and incident hypertension (indirect effect HR 1.09, 95%CI 1.08-1.10; direct effect: HR 1.08, 95%CI 1.02-1.15). The percentage of the mediation effect was 53.2% (95%CI 37.9-84.5). CONCLUSION: Hyperuricemia is associated with a risk of hypertension in both sexes, and BMI partially mediates hyperuricemia-related incident hypertension. OBJECTIVE sexspecific sex specific METHODS 31395 31 395 31,39 47.0% 470 47 0 (47.0 women disease followup follow up 200 2016 95 intervals RESULTS 2.9year 29year year 2.9 2 9 followup, up, 115 1 15 1.15 95CI CI 1.071.24 107124 1.07 1.24 07 24 1.07-1.2 participants 112 12 1.12 1.031.22 103122 1.03 1.22 03 22 1.03-1.2 men 123 23 1.23 1.021.48 102148 1.02 1.48 02 48 1.02-1.4 confounders 109 09 1.09 1.081.10 108110 1.08 1.10 08 10 1.08-1.1 107 0.981.16 098116 0.98 1.16 98 16 0.98-1.1 1.18 118 18 0.961.44 096144 0.96 1.44 96 44 0.96-1.4 women. . indirect 108 1.021.15. 102115 1.02-1.15) 532 53 53.2 37.984.5. 379845 37.9 84.5 37 84 5 37.9-84.5) CONCLUSION sexes hyperuricemiarelated related 3139 3 39 31,3 47.0 4 (47. 20 201 9year 29 2. 11 1.1 071 1.071.2 10712 1.0 124 1.2 1.07-1. 031 1.031.2 10312 103 122 1.03-1. 021 1.021.4 10214 102 148 1.4 1.02-1. 081 1.081.1 10811 110 1.08-1. 981 0.981.1 09811 098 0.9 116 0.98-1. 961 0.961.4 09614 096 144 0.96-1. 1.021.15 10211 1.02-1.15 53. 984 37.984.5 37984 379 37. 845 84. 8 37.9-84.5 313 31, 47. (47 1. 1.071. 1071 1.07-1 1.031. 1031 1.03-1 1.021. 1021 14 1.02-1 1.081. 1081 1.08-1 0.981. 0981 0. 0.98-1 0.961. 0961 0.96-1 1.021.1 1.02-1.1 37.984. 3798 37.9-84. (4 1.071 1.07- 1.031 1.03- 1.021 1.02- 1.081 1.08- 0.981 0.98- 0.961 0.96- 37.984 37.9-84 ( 37.98 37.9-8 37.9-

Abstract Pelvic inflammatory disease (PID) is an inflammation of the upper genital tract. PID is the leading cause of some severe sequelae in the absence of timely and accurate diagnosis and treatment. An appropriate animal model is needed to explore the underlying mechanism of PID sequelae. This study introduced an animal model of PID by vaginal injection of liquid Ureaplasma urealyticum combined with fatigue and hunger (UVF). This study was designed to test the feasibility of a rat model. A rat model was established using UVF irradiation. Levels of some inflammatory cytokines in the serum and the homogenates of the fallopian tubes were measured by ELISA, RT-PCR, and flow cytometry and compared with another rat model of Ureaplasma urealyticum liquids injected into the two uterus horns during laparotomy. Inflammatory alterations and adhesions were observed after hematoxylin and eosin (H&E) staining and detected using the Blauer scoring system. The results showed that the combined UVF and rat model caused apparent obstruction, edema, and adhesion in the fallopian tubes and connective tissues. The rat model showed upregulated CD4, CD8, and CD4/CD8 in peripheral blood mononuclear cells (PBMCs) and significantly increased levels of IL-4, IL-6, IL-10, and IL-17. UVF also enhanced the expression of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, vascular endothelial growth factor (VEGF) β, and matrix metalloproteinase (MMP)-2 (P<0.05). The UVF rat model can induce inflammatory alterations in the fallopian tubes and connective tissues, and can be used as a model of PID. (PID tract treatment UVF. . (UVF) irradiation ELISA RTPCR, RTPCR RT PCR, PCR RT-PCR laparotomy H&E HE H E (H&E system obstruction edema tissues CD4 CD CD8 CD4CD8 CDCD CD4/CD PBMCs (PBMCs IL4, IL4 IL 4, 4 IL-4 IL6, IL6 6, 6 IL-6 IL10, IL10 10, 10 IL-10 IL17. IL17 17. 17 IL-17 TNFα, TNFα TNF α, α (TNF)-α TGFβ, TGFβ TGF β (TGF)-β VEGF (VEGF MMP2 MMP 2 (MMP)- P<0.05. P005 P P<0.05 0 05 (P<0.05) (UVF CD4CD IL- IL1 1 IL-1 (MMP) P00 P<0.0 (P<0.05 (MMP P0 P<0. (P<0.0 P<0 (P<0. P< (P<0 (P< (P

Abstract Background The defect of B cell self-tolerance and the continuous antigen presentation by T cells (TCs) mediated by autoreactive B cells (BCs) play a key role in the occurrence and development of systemic lupus erythematosus (SLE). PD-1/PD-L1 signaling axis negatively regulates the immune response of TCs after activation and maintains immune tolerance. However, the effect of PD-1/PD-L1 signaling axis on the interaction between CD19+B/CD4+TCs in the peripheral blood of patients with SLE has not been studied in detail. Methods PD-1/PD-L1 and Ki-67 levels in peripheral blood (PB) of 50 SLE patients and 41 healthy controls (HCs) were detected through flow cytometry, and then the expression of PD-1+/−cells and PD-L1+/−cells Ki-67 was further analyzed. CD19+B/CD4+TCs were separated for cell culture and the supernatant was collected to determine proliferation and differentiation of TCs. IL-10 and IFN-γ secretion in the supernatant was also determined using ELISA. Results The PD-1, PD-L1, and Ki-67 levels on CD19+B/CD4+TCs in patients with SLE were higher than HCs. In CD19+B/CD4+TCs of SLE patients, the proliferative activity of PD-L1+ cells was higher than that of PD-L1− cells, and the proliferative activity of PD-1+ cells was higher than that of PD-1− cells. In the system co-culturing CD19+B/CD4+TCs from HCs/SLE patients, activated BCs promoted TCs proliferation and PD-L1 expression among TCs. Addition of anti-PD-L1 to co-culture system restored the proliferation of TCs, and inhibited IL-10/IFN-γ level. The addition of anti-PD-L1 to co-culture system also restored Tfh and downregulated Treg in HCs. Conclusions Axis of PD-1/PD-L1 on CD19+B/CD4+TCs in PB of SLE patients is abnormal, and cell proliferation is abnormal. In CD19+B/CD4+TCs of SLE patients, the proliferative activity of PD-L1+ and PD-1+ cells compared with PD-L1− and PD-1− cells in SLE patients, respectively. CD19+B/CD4+TCs in SLE patients can interact through PD-1/PD-L1. selftolerance self tolerance (TCs (BCs SLE. . (SLE) PD1/PDL1 PD1PDL1 PDPDL PD 1/PD L1 1 L PD-1/PD-L However CD19BCD4TCs CDBCDTCs CD19 CD4 CD detail Ki67 Ki 67 Ki-6 (PB 5 4 HCs (HCs cytometry PD1+/−cells PD1cells PDcells 1+/−cells PDL1+/−cells PDL1cells PDLcells L1+/−cells analyzed IL10 IL 10 IL-1 IFNγ IFN γ ELISA PD1, PD1 1, PD-1 PDL1, PDL1 PDL L1, PDL1+ L1+ PDL1− L1− PD1+ 1+ PD1− 1− coculturing co culturing HCsSLE PD-L antiPDL1 antiPDL anti anti-PD-L coculture IL10/IFNγ IL10IFNγ ILIFNγ 10/IFN level abnormal respectively PD1/PDL1. L1. (SLE PD1/PDL PD1PDL 1PD BCD CD1 Ki6 6 Ki- 1cells L1cells Lcells IL1 IL- PD- 10IFN