RESUMO: Este estudo tem como objetivo estabelecer uma estratégia terapêutica de leucopenia canina induzida pela infecção por parvovírus canino (CPV) através de infusão intravenosa de células tronco mesenquimais da medula óssea alogênica (BMMSCs) e avaliar o efeito terapêutico de BMMSCs no parvovírus canino. Quarenta cães saudáveis de dois meses de idade foram divididos aleatoriamente em quatro grupos: o grupo de tratamento de BMMSCs (A), o grupo de terapia convencional (B), o grupo de infecção por CPV (C) e um grupo controle (D). Os grupos A, B e C foram infectados oralmente com CPV (103.25 TCID50/mL) a 1mL/kg e o D recebeu a mesma dose de soro fisiológico. Após o início da infecção, o grupo A recebeu uma dose de derivadas da medula óssea (CTMMO) e hidratação como tratamento, o grupo B foi tratado com terapia anti-inflamatória e hidratação, e grupos C e D foram injetados com a mesma dose de soro fisiológico. As concentrações de IL-2 e IFN-α aumentaram gradualmente durante o tratamento, e o grupo de tratamento BMMSC mostrou concentrações significativamente maiores de IL-2 e IFN-α do que o grupo de tratamento convencional nos Dias 3 e 4. A expressão de vírus no sangue diminuiu gradualmente durante o tratamento, e o grupo de tratamento BMMSC mostrou uma diminuição mais rápida do que o grupo de tratamento convencional. Esses resultados mostraram as vantagens do tratamento BMMSC em relação ao tratamento convencional. Este estudo fornece uma nova estratégia de tratamento da BMMSC para leucopenia canina induzida pela infecção por VCP e revela o mecanismo pelo qual a BMMSC aumenta os leucócitos após infecção por VCP. RESUMO (CPV (BMMSCs , (A) B, (B) (C D. . (D) 103.25 10325 103 25 (103.2 TCID50/mL TCID50mL TCIDmL TCID50 mL TCID 1mLkg mLkg 1mL kg fisiológico CTMMO (CTMMO antiinflamatória anti inflamatória IL2 IL 2 IL- IFNα IFN α 4 (A (B (D 103.2 1032 10 (103. TCID5 103. 1 (103 (10 (1 (

ABSTRACT: This study aims to establish a therapy strategy for canine leukopenia induced by canine parvovirus (CPV) infection through intravenous infusion of allogeneic bone marrow mesenchymal stem cells (BMMSCs) and to evaluate the therapeutic effect of BMMSCs on canine parvovirus. Forty healthy 2-month-old dogs were randomly divided into four groups including the BMMSC treatment group (A), conventional treatment group (B), CPV infection group (C), and a normal control group (D). Then the A, B, and C groups were orally infected with CPV (103.25 TCID50/mL) at 1mL/kg, and the D group received the same dose of saline. After the onset of infection, Group A received mesenchymal stem cells (MSCs) and rehydration as the treatment; Group B was treated with anti-inflammatory therapeutics and rehydration; and Group C and D were injected with the same dose of physiological saline. The level of leukocytes rebounded significantly after the treatment with BMMSCs and returned to reference numbers on Day 3 after treatment, which was significantly higher than that in the conventional treatment group. The concentrations of IL-2 and IFN-α were gradually increased during the treatment, and the BMMSC treatment group exhibited significantly higher IL-2 and IFN-α concentrations than the conventional treatment group on Days 3 and 4. The expression of the virus in the blood gradually decreased during the treatment, and the BMMSC treatment group displayed a faster decrease than the conventional treatment group. These results showed the advantages of BMMSC treatment over conventional treatment. This study provides a new BMMSC treatment strategy for canine leukopenia induced by CPV infection and reveals the mechanism by which BMMSC increases leukocytes after CPV infection. ABSTRACT (CPV (BMMSCs 2monthold monthold 2 month old , (A) (B) C, (C) D. . (D) 103.25 10325 103 25 (103.2 TCID50/mL TCID50mL TCIDmL TCID50 mL TCID 1mLkg mLkg 1mL kg 1mL/kg saline MSCs (MSCs antiinflammatory anti inflammatory IL2 IL IL- IFNα IFN α 4 (A (B (C (D 103.2 1032 10 (103. TCID5 103. 1 (103 (10 (1 (

Resumo Nas últimas décadas, cresceu o interesse em compreender os mecanismos do desempenho da recuperação de serviço (DRS) na indústria hoteleira. Este estudo introduz uma perspectiva interativa que incorpora fatores extrínsecos e intrínsecos, investigando a influência da liderança inclusiva e da autonomia no trabalho na autoeficácia dos funcionários, sendo que a autoeficácia medeia a suas relações com o DRS. A atuação superficial é proposta como um moderador negativo. Questionários foram aplicados a uma amostra de 83 supervisores e 350 funcionários de front office de 8 hotéis chineses. As metodologias de análise baseiam-se no PLS-SEM e análise de regressão. Os resultados mostram que a liderança inclusiva e a autonomia no trabalho contribuem para uma autoeficácia forte e um DRS aprimorado. A atuação superficial modera a relação entre a liderança inclusiva e a autoeficácia, mas não entre a autonomia no trabalho e a autoeficácia. Esta pesquisa transversal oferece implicações práticas para os gestores hoteleiros, fornecendo orientações para promover ambientes que estimulem a autoeficácia e aprimorem o DRS. As limitações incluem a dependência de medidas autorrelatadas e um design transversal. Pesquisas futuras podem explorar outros moderadores e mediadores, diferentes setores industriais e utilizar designs longitudinais ou experimentais para estabelecer causalidade.

Abstract In recent decades, interest has grown in understanding the mechanisms of service recovery performance (SRP) in the hospitality industry. This study introduces an interactive perspective incorporating both extrinsic and intrinsic factors, investigating the roles of inclusive leadership and job autonomy on employee self-efficacy, with self-efficacy mediating their relationships with SRP. Surface acting is proposed as a negative moderator. A sample of 83 supervisors and 350 frontline employees from 8 Chinese hotels completed questionnaires. Data analysis was conducted using PLS-SEM in Smart PLS 4 and regression analysis in SPSS 25. Results show that inclusive leadership and job autonomy contribute to strong self-efficacy and enhanced SRP. Surface acting moderates the relationship between inclusive leadership and self-efficacy but not between job autonomy and self-efficacy. This cross-sectional survey offers practical implications for hospitality managers, providing guidance on fostering environments that promote self-efficacy and enhance SRP. Limitations include reliance on self-reported measures and a cross-sectional design. Future research could explore other moderators and mediators, different industries, and use longitudinal or experimental designs to establish causality.

Abstract The current developmental trend is to evaluate the quality of Yongchuan Xiuya tea rapidly. After spectrum pre-processing, near infrared spectroscopy (NIRS) coupled with synergy interval partial least squares (siPLS), principal component analysis (PCA) and back propagation-artificial neural network (BP-ANN) was applied to rapidly and non-destructively predict the quality of Yongchuan Xiuya tea. External Yongchuan Xiuya tea samples were used for the actual application of the proposed model. The best pre-processing method was multiple scattering correction coupled with second derivative, and the characteristic spectral regions selected by siPLS were 4381.5-4755.6 cm-1, 4759.5-5133.6 cm-1, 6266.6-6637.8 cm-1 and 7389.9-7760.2 cm-1. The cumulative contribution rate was 99.05% for the first three principal components of the characteristic spectra regions. The transfer function, root mean square error and determinant coefficient of the best BP-ANN prediction model were the tanh function, 0.384 and 0.977, respectively. The root mean square error and determinant coefficient of the external 10 Yongchuan Xiuya tea samples were 0.406 and 0.969, respectively. These results showed that NIRS combined with BP-ANN algorithm can be used to evaluate the quality of Yongchuan Xiuya tea rapidly and accurately.

Abstract This study aimed to investigate the effects of eugenol (Eu) at different concentrations (0, 10, 50, 100, 200, and 300 μM/g protein) on the gel properties and chemical structure changes of porcine myofibrillar protein (MP). The results showed that Eu inhibited the increase of surface hydrophobicity and carbonyl content but did not prevent the oxidation-induced loss of thiol groups. Moreover, Eu intensified the loss of the α-helical conformation as well as the tertiary structure of MP under oxidative stress. The physicochemical changes at 10 and 50 μM/g Eu resulted in a significant enhancement of the gelling ability of MP and enhanced the positive role of oxidation in building elastic gel networks. Conversely, Eu at the concentrations of 100, 200, and 300 μM/g was not conducive to gelling ability, especially at 300 μM/g, and these concentrations were associated with Eu-induced protein conformational changes. (Eu 0, 0 (0 100 200 30 μMg μM g MP. . (MP) oxidationinduced induced groups Moreover αhelical α helical stress 1 5 networks Conversely Euinduced ( 20 3 (MP 2

This study evaluated the effects of perioperative nutrition management by a multidisciplinary team on nutrition and postoperative complications of patients with esophageal cancer. A total of 239 patients with esophageal cancer who underwent esophagectomy and gastric conduit reconstruction for esophageal or esophagogastric junction cancer between February 2019 and February 2020 were included in the study. They were divided into the experimental group (120 patients) and the control group (119 patients) using the random number table method. Control group patients received routine diet management and experimental group patients received perioperative nutrition management by a multidisciplinary team. The differences of nutriture and postoperative complications between the two groups were compared. At 3 and 7 days after surgery, the experimental group patients had higher total protein and albumin levels (P<0.05), shorter postoperative anal exhaust time (P<0.05), lower incidence of postoperative gastrointestinal adverse reactions, pneumonia, anastomotic fistula, hypoproteinemia (P<0.05), and lower hospitalization costs (P<0.05) than the control group. Nutrition management by a multidisciplinary team effectively improved the nutriture of patients, promoted the rapid recovery of postoperative gastrointestinal function, reduced postoperative complications, and reduced hospitalization costs. 23 201 202 120 (12 119 (11 method compared surgery P<0.05, P005 P P<0.05 , 0 05 reactions pneumonia fistula (P<0.05 function 2 20 12 (1 11 P00 P<0.0 (P<0.0 1 ( P0 P<0. (P<0. P<0 (P<0 P< (P< (P

RESUMEN Introducción: La citoquina eritropoyetina (EPO), se conoce como una hormona crucial para la producción de glóbulos rojos, que transportan la sangre oxigenada al resto del cuerpo. Objetivos: El objetivo de este trabajo fue crear una detección electroquímica basada en nanopartículas de Fe2O3-NiO y óxido de grafeno para medir los niveles de EPO en la sangre de los deportistas. Métodos: Sobre un electrodo de carbono vítreo, se sintetizó Fe2O3-NiO@GO mediante el método de deposición electroquímica. Resultados: El Fe2O3-NiO@GO/GCE fue validado por caracterizaciones estructurales mediante microscopía electrónica de barrido (SEM). El Fe2O3-NiO@GO/GCE resultó ser un biosensor de eritropoyetina adecuado y estable con un rango lineal de 0-500 ng/l y un límite de detección de 0,03ng/l en ensayos electroquímicos mediante la técnica DPV. Se investigó el uso de Fe2O3-NiO@GO/extropoietina como biosensor de eritropoyetina en el plasma de atletas. Conclusión: Los resultados mostraron que los valores obtenidos para la recuperación (94,56% a 98,40) y la RSD (2,01% a 3,22%) fueron aceptables, lo que indica que la técnica sugerida puede ser utilizada como un biosensor práctico de eritropoyetina en muestras de sangre. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción EPO, , (EPO) rojos cuerpo Objetivos Fe2O3NiO FeONiO Fe2O3 NiO Fe O deportistas Métodos vítreo Fe2O3NiO@GO Fe2O3NiOGO FeONiOGO NiO@GO GO Resultados Fe2O3NiO@GO/GCE Fe2O3NiOGOGCE FeONiOGOGCE NiO@GO/GCE GCE SEM. SEM . (SEM) 0500 0 500 0-50 ngl ng l 003ngl 03ng DPV Fe2O3NiO@GO/extropoietina Fe2O3NiOGOextropoietina FeONiOGOextropoietina NiO@GO/extropoietina extropoietina atletas Conclusión 94,56% 9456 94 56 (94,56 98,40 9840 98 40 2,01% 201 2 01 (2,01 3,22% 322 3 22 aceptables II tratamiento (EPO FeO Fe2O NiOGO NiOGOGCE (SEM 050 50 0-5 NiOGOextropoietina 94,56 945 9 5 (94,5 98,4 984 4 2,01 20 (2,0 3,22 32 05 0- 94,5 (94, 98, 2,0 (2, 3,2 94, (94 2, (2 3, (9 (

RESUMO Introdução: A citocina eritropoietina (EPO), é referida como um hormônio crucial para a produção de hemácias, que transportam o sangue oxigenado para o resto dos corpos. Objetivos: O objetivo deste trabalho foi criar uma detecção eletroquímica baseada em nanopartículas de Fe2O3-NiO e óxido de grafeno para medir os níveis de EPO no sangue dos atletas. Métodos: Em um eletrodo de carbono vítreo, o Fe2O3-NiO@GO foi sintetizado usando o método de deposição eletroquímica. Resultados: O Fe2O3-NiO@GO/GCE foi validado por caracterizações estruturais utilizando microscopia eletrônica de varredura (SEM). O Fe2O3-NiO@GO/GCE foi considerado um biosensor eritropoietina adequado e estável com uma faixa linear de 0-500 ng/l e um limite de detecção de 0,03ng/l em testes eletroquímicos utilizando a técnica DPV. O Fe2O3-NiO@GO/extropoietina foi investigado como um biosensor de eritropoietina no plasma do atleta. Conclusão: Os resultados mostraram que os valores obtidos para recuperação (94,56% a 98,40) e RSD (2,01% a 3,22%) foram aceitáveis, indicando que a técnica sugerida pode ser usada como um prático biosensor de eritropoietina em amostras de sangue. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução EPO, , (EPO) hemácias corpos Objetivos Fe2O3NiO FeONiO Fe2O3 NiO Fe atletas Métodos vítreo Fe2O3NiO@GO Fe2O3NiOGO FeONiOGO NiO@GO GO Resultados Fe2O3NiO@GO/GCE Fe2O3NiOGOGCE FeONiOGOGCE NiO@GO/GCE GCE SEM. SEM . (SEM) 0500 0 500 0-50 ngl ng l 003ngl 03ng DPV Fe2O3NiO@GO/extropoietina Fe2O3NiOGOextropoietina FeONiOGOextropoietina NiO@GO/extropoietina extropoietina atleta Conclusão 94,56% 9456 94 56 (94,56 98,40 9840 98 40 2,01% 201 2 01 (2,01 3,22% 322 3 22 aceitáveis II tratamento (EPO FeO Fe2O NiOGO NiOGOGCE (SEM 050 50 0-5 NiOGOextropoietina 94,56 945 9 5 (94,5 98,4 984 4 2,01 20 (2,0 3,22 32 05 0- 94,5 (94, 98, 2,0 (2, 3,2 94, (94 2, (2 3, (9 (

ABSTRACT Introduction: The cytokine erythropoietin (EPO) is a crucial hormone for producing RBCs, which carry oxygenated blood to the rest of the body. Objective: This paper aimed to create an electrochemical detection based on Fe2O3-NiO nanoparticles and graphene oxide to measure EPO levels in athletes’ blood. Methods: On a glassy carbon electrode, Fe2O3-NiO@GO was synthesized using the electrochemical deposition method. Results: The Fe2O3-NiO@GO/GCE was validated by structural characterizations using scanning electron microscopy (SEM). The Fe2O3-NiO@GO/GCE was found to be a suitable and stable erythropoietin biosensor with a linear range of 0-500 ng/l and a detection limit of 0.03ng/l in electrochemical tests using the DPV technique. Fe2O3-NiO@GO/erythropoietin was investigated as a biosensor for erythropoietin in athlete's plasma. Conclusion: The results showed that the values obtained for recovery (94.56% to 98.40) and RSD (2.01% to 3.22%) were acceptable, indicating that the suggested technique can be used as a practical erythropoietin biosensor in blood samples. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction (EPO RBCs body Objective Fe2O3NiO FeONiO Fe2O3 NiO Fe O athletes Methods electrode Fe2O3NiO@GO Fe2O3NiOGO FeONiOGO NiO@GO GO method Results Fe2O3NiO@GO/GCE Fe2O3NiOGOGCE FeONiOGOGCE NiO@GO/GCE GCE SEM. SEM . (SEM) 0500 0 500 0-50 ngl ng l 003ngl 03ng Fe2O3NiO@GO/erythropoietin Fe2O3NiOGOerythropoietin FeONiOGOerythropoietin NiO@GO/erythropoietin athlete s plasma Conclusion 94.56% 9456 94 56 (94.56 98.40 9840 98 40 2.01% 201 2 01 (2.01 3.22% 322 3 22 acceptable samples II outcomes FeO Fe2O NiOGO NiOGOGCE (SEM 050 50 0-5 NiOGOerythropoietin 94.56 945 9 5 (94.5 98.4 984 4 2.01 20 (2.0 3.22 32 05 0- 94.5 (94. 98. 2.0 (2. 3.2 94. (94 2. (2 3. (9 (

Abstract Sojae semen germinatum (SSG) prepared from the germinating black soybean Glycine max L. Merr. is a traditional Chinese herbal medicine with heat-clearing, dampness-removing, and detoxifying properties. In the present study, we explored the effects and possible underlying mechanisms of petroleum ether extract (PEE), ethyl acetate extract (EAE), n-butanol extract (NBE) and aqueous fraction (AF) of SSG on the carbon tetrachloride (CCl4)-induced liver injury in mice, an animal model representing pathological features of “dampness-heat” syndrome from the perspective of Traditional Chinese Medicine. Results showed that the EAE and NBE of SSG significantly protected mice against the CCl4-induced increases of liver tissue index and serum transaminase levels, ameliorated the CCl4-induced liver morphological changes. Compared with CCl4 model controls, EAE and NBE of SSG decreased malondiadehyde (MDA) levels, enhanced glutathione (GSH) content and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver tissues, as well as reduced the number of apoptotic hepatocytes. Moreover, the CCl4-induced increase of pro-inflammatory cytokine levels in livers was markedly suppressed by pre-treatment with EAE of SSG. Data suggested that SSG extracts showed protective effect on the CCl4-induced liver injury via multiple pharmacological mechanisms including anti-oxidation, anti-inflammation and anti-apoptosis. EAE and NBE appeared to be superior to other fractions. (SSG L Merr heatclearing, heatclearing heat clearing, clearing heat-clearing dampnessremoving, dampnessremoving dampness removing, removing dampness-removing properties study PEE, PEE , (PEE) EAE, (EAE) nbutanol n butanol (NBE AF (AF CCl4induced CClinduced induced CCl dampnessheat “dampness-heat Medicine changes controls MDA (MDA GSH (GSH SOD (SOD GSHPx Px (GSH-Px tissues hepatocytes Moreover proinflammatory pro inflammatory pretreatment pre treatment antioxidation, antioxidation anti oxidation, oxidation anti-oxidation antiinflammation inflammation antiapoptosis. antiapoptosis apoptosis. apoptosis anti-apoptosis fractions (PEE (EAE

Resumo Fundamento A estratificação de risco precoce com biomarcadores simples é essencial em pacientes com infarto do miocárdio sem supradesnivelamento do segmento ST (IAMSSST). Objetivo Este estudo tem o objetivo de avaliar a associação entre nível de big endotelina-1 plasmática (ET-1) e o escore SYNTAX (SS) em pacientes com IAMSSST. Métodos Foram recrutados 766 pacientes com IAMSSST que passaram por angiografia coronária. Os pacientes foram divididos em três grupos: SS baixo (≤22), SS intermediário (23-32), e SS alto (>32). A correlação de Spearman, o ajuste de curva suave, a regressão logística, e a análise de curva característica de operação do receptor (ROC) foram realizados para avaliar a associação entre o nível de big ET-1 plasmática e o SS. Um p-valor <0.05 foi considerado estatisticamente significativo. Resultados Foi identificada uma correlação significativa entre a big ET-1 e o SS (r=0,378, p<0,001). A curva suavizada indicou uma correlação positiva entre o nível de big ET-1 plasmática e o SS. A análise de curva ROC demonstrou que a área sob a curva foi de 0,695 (0,661-0,727) e o ponto de corte ideal do nível de big ET-1 plasmática foi de 0,35 pmol/l. A regressão logística demonstrou que a big ET-1 elevada era um preditor independente de SS intermediário a alto em pacientes com IAMSSST, seja como variável contínua [RC (IC 95%: 1,110 (1,053-1,170), p<0,001] ou como variável categórica [RC (IC 95%: 2,962 (2,073-4,233), p<0,001]. Conclusão Em pacientes com IAMSSST, o nível de big ET-1 plasmática estava significativamente correlacionado ao SS. O nível de big ET-1 plasmática elevado foi um preditor independente para SS intermediário a alto. . (IAMSSST) endotelina1 endotelina 1 endotelina- ET1 ET (ET-1 (SS 76 coronária grupos ≤22, 22 ≤22 , (≤22) 2332, 2332 23 32 (23-32) >32. >32 (>32) Spearman suave (ROC ET- pvalor p valor 005 0 05 <0.0 significativo r=0,378, r0378 r 378 (r=0,378 p<0,001. p0001 p<0,001 001 p<0,001) 0695 695 0,69 0,6610,727 06610727 0,661 0,727 661 727 (0,661-0,727 035 35 0,3 pmoll pmol l pmol/l RC IC 95% 95 1110 110 1,11 1,0531,170, 10531170 1,053 1,170 053 170 (1,053-1,170) 2962 2 962 2,96 2,0734,233, 20734233 2,073 4,233 073 4 233 (2,073-4,233) (IAMSSST (ET- 7 ≤2 (≤22 3 (23-32 >3 (>32 00 <0. r=0,378 r037 37 (r=0,37 p000 p<0,00 069 69 0,6 6610 0,6610,72 0661072 0661 0,66 0727 0,72 66 72 (0,661-0,72 03 0, 9 111 11 1,1 0531 1,0531,170 1053117 1053 1,05 1170 1,17 17 (1,053-1,170 296 96 2,9 0734 2,0734,233 2073423 2073 2,07 4233 4,23 07 (2,073-4,233 (ET ≤ (≤2 (23-3 > (>3 <0 r=0,37 r03 (r=0,3 p00 p<0,0 06 6 0,6610,7 066107 066 072 0,7 (0,661-0,7 1, 1,0531,17 105311 105 1,0 117 (1,053-1,17 29 2, 2,0734,23 207342 207 2,0 423 4,2 (2,073-4,23 (≤ (23- (> < r=0,3 r0 (r=0, p0 p<0, 0,6610, 06610 (0,661-0, 1,0531,1 10531 10 (1,053-1,1 2,0734,2 20734 20 42 4, (2,073-4,2 ( (23 r=0, (r=0 p<0 0,6610 (0,661-0 1,0531, (1,053-1, 2,0734, (2,073-4, (2 r=0 (r= p< (0,661- 1,0531 (1,053-1 2,0734 (2,073-4 r= (r (0,661 (1,053- (2,073- (0,66 (1,053 (2,073 (0,6 (1,05 (2,07 (0, (1,0 (2,0 (0 (1, (2, (1

Abstract Background Early risk stratification with simple biomarkers is essential in patients with non-ST segment-elevation myocardial infarction (NSTEMI). Objective This study aimed to evaluate the association between plasma big endothelin-1 (ET-1) level and the SYNTAX score (SS) in patients with NSTEMI. Methods A total of 766 patients with NSTEMI undergoing coronary angiography were recruited. Patients were divided into three groups: low SS (≤22), intermediate SS (23-32), and high SS (>32). Spearman correlation, smooth curve fitting, logistic regression, and receiver operating characteristic (ROC) curve analysis were performed to evaluate the association between plasma big ET-1 level and the SS. A p-value <0.05 was considered statistically significant. Results There was a significant correlation between the big ET-1 and the SS (r=0.378, p<0.001). The smoothing curve indicated a positive correlation between the plasma big ET-1 level and the SS. The ROC curve analysis showed that the area under the curve was 0.695 (0.661-0.727) and the optimal cutoff of plasma big ET-1 level was 0.35pmol/l. Logistic regression showed that elevated big ET-1 was an independent predictor of intermediate-high SS in patients with NSTEMI, whether entered as a continuous variable [OR (95% CI): 1.110 (1.053-1.170), p<0.001] or as a categorical variable [OR (95% CI): 2.962 (2.073-4.233), p<0.001]. Conclusion In patients with NSTEMI, the plasma big ET-1 level was significantly correlated with the SS. Elevated plasma big ET-1 level was an independent predictor for intermediate-high SS. nonST non ST segmentelevation segment elevation . (NSTEMI) endothelin1 endothelin 1 endothelin- ET1 ET (ET-1 (SS 76 recruited groups ≤22, 22 ≤22 , (≤22) 2332, 2332 23 32 (23-32) >32. >32 (>32) fitting (ROC ET- pvalue p value 005 0 05 <0.0 r=0.378, r0378 r 378 (r=0.378 p<0.001. p0001 p<0.001 001 p<0.001) 0695 695 0.69 0.6610.727 06610727 0.661 0.727 661 727 (0.661-0.727 035pmoll pmoll 35pmol l pmol 0.35pmol/l intermediatehigh OR 95% 95 (95 CI CI) 1110 110 1.11 1.0531.170, 10531170 1.053 1.170 053 170 (1.053-1.170) 2962 2 962 2.96 2.0734.233, 20734233 2.073 4.233 073 4 233 (2.073-4.233) (NSTEMI (ET- 7 ≤2 (≤22 3 (23-32 >3 (>32 00 <0. r=0.378 r037 37 (r=0.37 p000 p<0.00 069 69 0.6 6610 0.6610.72 0661072 0661 0.66 0727 0.72 66 72 (0.661-0.72 9 (9 111 11 1.1 0531 1.0531.170 1053117 1053 1.05 1170 1.17 17 (1.053-1.170 296 96 2.9 0734 2.0734.233 2073423 2073 2.07 4233 4.23 07 (2.073-4.233 (ET ≤ (≤2 (23-3 > (>3 <0 r=0.37 r03 (r=0.3 p00 p<0.0 06 6 0. 0.6610.7 066107 066 072 0.7 (0.661-0.7 ( 1. 1.0531.17 105311 105 1.0 117 (1.053-1.17 29 2. 2.0734.23 207342 207 2.0 423 4.2 (2.073-4.23 (≤ (23- (> < r=0.3 r0 (r=0. p0 p<0. 0.6610. 06610 (0.661-0. 1.0531.1 10531 10 (1.053-1.1 2.0734.2 20734 20 42 4. (2.073-4.2 (23 r=0. (r=0 p<0 0.6610 (0.661-0 1.0531. (1.053-1. 2.0734. (2.073-4. (2 r=0 (r= p< (0.661- 1.0531 (1.053-1 2.0734 (2.073-4 r= (r (0.661 (1.053- (2.073- (0.66 (1.053 (2.073 (0.6 (1.05 (2.07 (0. (1.0 (2.0 (0 (1. (2. (1

SUMMARY: This study is to investigate the regulation of Notch1 and Foxp1 by miR-34a in the development of psoriasis vulgaris. RT-PCR was used to compare the levels of miR-34a in the skin lesions of 20 patients with psoriasis vulgaris and 20 normal skin tissues. Immunohistochemistry was used to detect the expression of Notch1 and Foxp1 in 51 patients with psoriasis vulgaris, which were further compared with that in 29 normal control tissues. In addition, in HaCaT cells, we used miR-34a mimics and inhibitors to overexpress and inhibit miR-34a, respectively, and detected the mRNA and protein levels of miR-34a, Notch1, and Foxp1. The level of miR-34a in the skin lesions of patients with psoriasis vulgaris was significantly higher than that in normal skin tissues (t=2.192, P<0.05). The positive rate of Notch1 in the skin lesions of patients with psoriasis vulgaris was 76.47 %, which was significantly higher than that in normal skin tissues (13.79 %) (t=29.215, P<0.01). The positive rate of FOXP1 in the psoriasis vulgaris group was 92.16 %, which was also significantly higher than that in the normal skin group (65.52 %) (t=9.087, P<0.01). In addition, overexpression of miR-34a significantly promoted the expression of Notch1 and Foxp1. However, inhibition of miR-34a significantly reduced Notch1 and Foxp1 levels. miR- 34a is highly expressed in the skin tissues of patients with psoriasis vulgaris, and may participate in the development of psoriasis vulgaris by regulating Notch1 and Foxp1.

RESUMEN: El objetivo de este estudio fue investigar la regulación de Notch1 y Foxp1 por miR-34a en el desarrollo de la psoriasis vulgar. Se utilizó RT-PCR con el fin de comparar los niveles de miR-34a en las lesiones cutáneas de 20 pacientes con psoriasis vulgar y 20 tejidos de piel normales. Se utilizó inmunohistoquímica para detectar la expresión de Notch1 y Foxp1 en 51 pacientes con psoriasis vulgar, que se compararon además con la de 29 tejidos normales control. Además, en las células HaCaT, usamos miméticos e inhibidores de miR-34a para sobreexpresar e inhibir miR-34a, respectivamente, y detectamos los niveles de ARNm y proteína de miR-34a, Notch1 y Foxp1. El nivel de miR- 34a en las lesiones cutáneas de pacientes con psoriasis vulgar fue significativamente mayor que en los tejidos normales de la piel (t=2,192, P<0,05). La tasa de positividad de Notch1 en las lesiones cutáneas de pacientes con psoriasis vulgar fue del 76,47 %, que fue significativamente mayor que la de los tejidos normales de la piel (13,79 %) (t=29,215, P<0,01). La tasa positiva de FOXP1 en el grupo de psoriasis vulgar fue del 92,16 %, que también fue significativamente mayor que la del grupo de piel normal (65,52 %) (t=9,087, P<0,01). Además, la sobreexpresión de miR-34a promovió significativamente la expresión de Notch1 y Foxp1. Sin embargo, la inhibición de miR-34a redujo de manera importante los niveles de Notch1 y Foxp1. miR-34a se expresa en gran medida en los tejidos de la piel en pacientes con psoriasis vulgar y puede participar en el desarrollo de la psoriasis vulgar mediante la regulación de Notch1 y Foxp1.

Abstract In this study, through animal experiments, we investigated the effects of Qingli Gandan decoction on the IL-23/IL-17 signalling pathway and the expression of CD4+/IL-17A+ cells in EAU rats. We then predicted the potential mechanisms of the action. First, Lewis rats were divided using the random number table method into a (1) control group, (2) model group and (3) TCM group. The TCM group was given a Qingli Gandan prescription by gavage after an intervention model. Next, blood, aqueous humour and vitreous were collected from each group on days 4, 8, 12, 16 and 20 after immunisation. An enzyme linked immunosorbent assay (ELISA) was used to detect the protein concentrations of IL-17 and IL-23 in a serum, aqueous humour and vitreous of rats on different immunisation days, and the percentage of CD4+/IL-17A+ cells in rat spleen was detected by flow cytometry. The peak concentration of serum IL-17 protein in group 3 was statistically lower than that in group 2 (P < 0.05) on days 4, 8 and 12 of immunisation. However, the peak concentration of serum IL-23 protein in group 3 was statistically lower than in group 2 (P < 0.05) on days 8 and 12 of immunisation; in addition, on days 8 and 12, the protein concentrations of IL-23 and IL-17 in the aqueous humour group 3 were all lower than those in group 2. On days 4, 8 and 12, the protein concentrations of IL-23 and IL-17 in vitreous in group 3 were significantly lower than those in group 2. We found that the proportion of CD4+/IL-17A+ cells in the groups 2 and 3 increased on day 8 compared with group 1 (P < 0.05). The proportion of CD4+/IL-17A+ cells decreased in group 3 compared with group 2 (P < 0.05).At last, we think Qingli Gandan can reduce the onset of EAU in rats and treat it, presumably because of inhibiting the IL-23/IL-17 axis. It may inhibit the expression of CD4+/IL-17A+ cells and play a role in inhibiting the IL-23/IL-17 signalling pathway.

Abstract Through in vitro experiments, the paper investigated whether Lycopene can mitigate cardiac ischemia/reperfusion injury, and further explored whether the underlying mechanism works by inhibiting the opening of MPTP.A hypoxia-reoxygenation model was established. CCK8 was employed to determine the viability of H9c2 cardiomyocytes. LDH was used to determine the degree of damage of H9c2 cardiomyocytes. Trypan blue staining was applied to determine the survival rate of H9c2 cardiomyocytes. Flow cytometry was used to determine the apoptosis rate of H9c2 cardiomyocytes. Rhodamine 123 staining method was applied to evaluate the changes of mitochondrial membrane potential of H9c2 cardiomyocytes. Western blot was employed to determine the protein expressions of related factors of MPTP downstream apoptosis pathway in H9c2 cardiomyocytes. Calcium induction method was used to evaluate the opening degree of MPTP of H9c2 cardiomyocytes. Western blot was applied to determine the protein expressions of the related factors regulating MPTP opening in H9c2 cardiomyocytes.The vitality of H9c2 cardiomyocytes in the Lycopene group was significantly improved, the LDH activity was significantly reduced, and the cell survival rate was significantly improved. The apoptosis rate was significantly reduced, and the membrane potential decreased significantly. The expression levels of Bax, cytochrome C, APAF-1, caspase 9 and caspase-3 proteins were significantly reduced. The sensitivity of MPTP opening was significantly decreased. Lycopene can effectively alleviate the hypoxia/reoxygenation injury of H9c2 cardiomyocytes. By up-regulating Bcl-2 and down-regulating Bax, Lycopene can inhibit the opening of mitochondrial MPTP and the activation of downstream apoptotic pathways, thereby reducing apoptosis.

Abstract Benign recurrent meningitis, also known as Mollaret’s meningitis (MM), is characterized by recurrent headache, fever, meningeal irritation and sterility of cerebrospinal fluid examination, which is rare in clinic. Although with clear diagnostic criteria, there are still many unclear aspects of its pathogenesis, and treatment of this disease. The author reported a case of benign recurrent aseptic meningitis, who had three episodes of acute headache with or without fever. The patient was treated with ceftriaxone, acyclovir and cefazoxime for the first time, cefazoxime, cefimidazole and mebendazole for the second time, and piperacillin and acyclovir for the third time. The results showed that the patient did not recover completely in the first two times and fully recovered at the third time. Based on the current research, the author believes that for MM, mainly symptomatic relief, indomethacin should be recommended, and too much antiviral treatment is not needed.

Abstract Flaxseed (Linum usitatissimum L.), one kind of common oilseeds, has many biologically active compounds including α-linolenic acid (ALA), lignan and dietary fiber. At present, the research of flaxseed is focused on biological and clinical studies of its compounds. However, the studies of flaxseed protein obtained generally from flaxseed cake, are relatively limited. As many other kinds of plant proteins, flaxseed protein presents many excellent functionalities which can be used for food applications. Many evidence have showed that flaxseed protein hydrolysates can provide health benefits to our body, such as anti-bacterial activity, antioxidant capacity and angiotensin-converting enzyme inhibition ability. Therefore, in order to make better use of flaxseed protein in foods, the extraction, functionalities and applications of flaxseed protein were reviewed in this paper. In addition, the preparation of flaxseed protein hydrolysates was discussed.

Abstract Anthocyanins from Lycium ruthenicum Murry (LRAs) were used to explore the anti-fatigue activities of LRAs at different concentrations in a mouse fatigue model. Eight biochemical indexes were determined in liver tissue, muscle tissue and serum. The results showed that the LRAs used in the experiment mainly contained delphinium, petunidin and malvidin. During 21 days of gavage, there was no significant difference in mouse body weight. The exercise time of the LRA-exposed mice increased. LRAs could increase serum glucose (Glu), liver/muscle glycogen and the activity of superoxide dismutase (SOD) and reduce the vitality of lactate dehydrogenase (LDH), the content of malondialdehyde (MDA), the levels of lactic acid (LD) and serum urea nitrogen (BUN). This study indicated that LRAs can enhance exercise endurance, increase sugar reserves, scavenge free radicals and improve metabolism, thereby increasing anti-fatigue activities.

Abstract Quinoa starch microparticles (QSMs) fabricated with unhydrolyzed quinoa starch (QS) by inverse microemulsion technology could be used as medicine delivery and cosmetic accessories. The size distribution of QSMs was uniform at 28.5 μM, and the adsorption capacity of methylene blue was 0.82 mg/ g. The optimum preparation process was as follows: a QS mass fraction of 9%, an epoxy chloropropane content of 1%, an amount of oil phase of 100 mL, a span 80 of 3 mg/mL, and a stirring speed of 400 r/min for 3 h at room temperature and pressure. The QSMs were spherical, ellipsoidal in shape with the specific surface area of 1.676 m2/g. The crystallinity of the QSMs increased from 1.24% to 16.8% after crosslinking. The maximum thermal degradation temperature of the QSMs rose slightly than QS. It will be a promising material for application as a drug carrier and in cosmetics.