Abstract Background: Anemia is common in the preoperative setting and associated with increased postoperative complications and mortality. However, it is unclear if preoperative anemia optimization reduces postoperative complications. We aimed to assess the association between preoperative anemia optimization and a composite endpoint of major cardiovascular, renal, and pulmonary complications and all-cause mortality within 30 days after noncardiac surgery in adult patients. Methods: In this retrospective analysis preoperative anemia was defined as hemoglobin concentration below 12.0 g.dl−1 in women and 13.0 g.dl−1 in men within 6 months before surgery. A propensity score-based generalized estimating equation analysis was used to determine the association between preoperative anemia optimization and the primary outcome. Moreover, mediation analysis was conducted to investigate whether intraoperative red blood cell transfusion or duration of intraoperative hypotension were mediators of the relation between anemia optimization and the primary outcome. Results: Fifty-seven hundred anemia optimized, and 8721 non-optimized patients met study criteria. The proportion of patients having any component of the composite of major complications and all-cause mortality was 21.5% in the anemia-optimized versus 18.0% in the non-optimized, with confounder-adjusted odds ratio estimate of 0.99 (95% CI 0.86–1.15) for anemia optimization versus non-optimization, p = 0.90. Intraoperative red blood cell transfusion had a minor mediation effect on the relationship between preoperative anemia optimization and the primary outcome, whereas duration of intraoperative hypotension was not found to be a mediator. Conclusion: Preoperative anemia optimization did not appear to be associated with a composite outcome of major in-hospital postoperative cardiovascular, renal, and pulmonary complications and all-cause in-hospital mortality. Background However cardiovascular renal allcause all cause 3 Methods 120 12 0 12. gdl1 gdl g dl 1 g.dl− 130 13 13. scorebased score based Moreover Results Fiftyseven Fifty seven optimized 872 nonoptimized non criteria 215 21 5 21.5 anemiaoptimized 180 18 18.0 nonoptimized, confounderadjusted confounder adjusted 099 99 0.9 95% 95 (95 0.86–1.15 086115 86 15 nonoptimization, nonoptimization optimization, non-optimization 090 90 0.90 mediator Conclusion inhospital hospital g.dl 87 2 21. 18. 09 9 0. (9 0.86–1.1 08611 8 ( 0.86–1. 0861 0.86–1 086 0.86– 08 0.86 0.8

Abstract Aims Very Low Birthweight (VLBW) infants with neonatal Chronic Lung Disease (CLD) have been found to have functional impairment of the brainstem auditory pathway at term. This study investigated the functional status of the brainstem auditory pathway in VLBW infants with CLD after term for any abnormality. Methods Fifty-two VLBW infants were recruited at 50 weeks of Postconceptional Age: 25 with neonatal CLD and 27 without CLD. None had any other major complications to minimize confounding effects. Brainstem Auditory Evoked Responses were studied at 21‒91/s click rates. Results Compared with those without CLD, VLBW infants with CLD had relatively shorter latencies of BAER waves I and III, associated with a slightly lower BAER threshold. Wave V latency and I‒V interpeak interval did not differ significantly between the two groups of infants. The I‒III interval in infants with CLD was shorter than in those without CLD at 91/s clicks. However, the III‒V interval was significantly longer than in those without CLD at all click rates (all p < 0.05). There were no significant differences in the amplitudes of BAER wave components between the two groups of infants. Conclusions The main BAER abnormality in VLBW infants with CLD was a prolonged III‒V interval. Auditory conduction is delayed or impaired at more central regions of the brainstem in CLD infants. After term central auditory function is adversely affected by neonatal CLD. Monitoring post-term change is required to provide valuable information for post-term care of CLD infants. (VLBW (CLD Fiftytwo Fifty 5 Age 2 effects 2191s s 21 91 III threshold IV IIII 91s clicks However IIIV 0.05. 005 0.05 . 0 05 0.05) postterm post 9 00 0.0 0.

ABSTRACT There are significant differences in the quantities of Camellia oleifera fruits growing within and outside the canopy distribution, meaning that a single mode of picking has drawbacks. To improve the harvesting efficiency for the inner and outer layers, this article proposes a synergistic mode of harvesting based on a vibration comb brush, and presents a design for a harvesting device based on this principle. The overall structure and working principle of the proposed device are explained, and the operational processes of the vibration and comb parts of the device are analyzed. ADAMS software is used to construct a rigid-flexible coupling model of the device and the fruiting branch, and simulation results are presented to show that the fruit drop and flower loss rates for Camellia oleifera are related to the vibration frequency, the amplitude, and the spacing between the teeth and comb plates. Finally, a three factor and three level field orthogonal experiment was conducted, and the results showed that under the conditions of vibration frequency 5.85Hz, amplitude 60.43mm, and comb spacing 45mm, the flower loss rate was the lowest and it had good picking performance. Under these conditions, the fruit drop rate is 87.32% and the flower loss rate is 8.06%, values that meet the requirements for mechanized picking of Camellia oleifera fruits. distribution drawbacks layers brush explained analyzed rigidflexible rigid flexible branch plates Finally conducted 585Hz Hz 5 85Hz 5.85Hz 6043mm mm 60 43mm 60.43mm 45mm performance 8732 87 32 87.32 806 8 06 8.06% 6 873 3 87.3 80 0 8.06 87. 8.0 8.

RESUMO Um March Golden macho, pesando 7,6kg, apresentou perda de peso gradual, temperatura corporal alta, depressão, falta de apetite e sede, e vômitos antes da consulta. Os resultados mostraram que os eritrócitos, hematócrito, hemoglobina e plaquetas eram menores que os valores de referência. O diagnóstico de infecção mista com hematócrito e corpos eosinófilos foi confirmado por PCR de fluorescência em tempo real de sangue total, o que foi positivo para hematócrito e corpos eosinófilos. O cão foi tratado com doxiciclina e ceftriaxona, e o cão recuperou-se completamente após duas semanas com transfusão de sangue, tratamento sintomático e terapia de suporte. Isto indica que a doença pode ser bem tratada através de uma abordagem de tratamento abrangente. macho 76kg kg 7 6kg 7,6kg gradual alta depressão sede consulta eritrócitos referência total ceftriaxona recuperouse recuperou se suporte abrangente

ABSTRACT A March male Golden, weighing 7.6kg, presented with gradual weight loss, high body temperature, depression, poor appetite and thirst, and vomiting before consultation. The results showed that the erythrocytes, hematocrit, hemoglobin, and platelets were lower than the reference values. The diagnosis of mixed infection with haematocrit and eosinophilic bodies was confirmed by real-time fluorescence PCR of whole blood, which was positive for haematocrit and eosinophilic bodies. The dog was treated with doxycycline and ceftriaxone, and the dog fully recovered after 2 weeks with blood transfusion, symptomatic treatment, and supportive therapy. This indicates that the disease can be treated well by a comprehensive treatment approach. Golden 76kg kg 7 6kg 7.6kg loss temperature depression thirst consultation erythrocytes hematocrit hemoglobin values realtime real time ceftriaxone transfusion therapy approach

ABSTRACT BACKGROUND: Computer-aided diagnosis in low-dose (≤ 3 mSv) computed tomography (CT) is a potential screening tool for lung nodules, with quality interpretation and less inter-observer variability among readers. Therefore, we aimed to determine the screening potential of CT using a radiation dose that does not exceed 2 mSv. OBJECTIVE: We aimed to compare the diagnostic parameters of low-dose (< 2 mSv) CT interpretation results using a computer-aided diagnosis system for lung cancer screening with those of a conventional reading system used by radiologists. DESIGN AND SETTING: We conducted a comparative study of chest CT images for lung cancer screening at three private institutions. METHODS: A database of low-dose (< 2 mSv) chest CT images of patients at risk of lung cancer was viewed with the conventional reading system (301 patients and 226 nodules) or computer-aided diagnosis system without any subsequent radiologist review (944 patients and 1,048 nodules). RESULTS: The numbers of detected and solid nodules per patient (both P < 0.0001) were higher using the computer-aided diagnosis system than those using the conventional reading system. The nodule size was reported as the maximum size in any plane in the computer-aided diagnosis system. Higher numbers of patients (102 [11%] versus 20 [7%], P = 0.0345) and nodules (154 [15%] versus 17 [8%], P = 0.0035) were diagnosed with cancer using the computer-aided diagnosis system. CONCLUSIONS: The computer-aided diagnosis system facilitates the diagnosis of cancerous nodules, especially solid nodules, in low-dose (< 2 mSv) CT among patients at risk for lung cancer. BACKGROUND Computeraided Computer aided lowdose low ≤ ( mSv (CT interobserver inter observer readers Therefore OBJECTIVE computeraided computer radiologists SETTING institutions METHODS 301 (30 22 944 (94 1048 1 048 1,04 nodules. . RESULTS both 0.0001 00001 0 0001 102 (10 11% 11 [11% 7%, 7 7% , [7%] 0.0345 00345 0345 154 (15 15% 15 [15% 8%, 8 8% [8%] 0.0035 00035 0035 CONCLUSIONS 30 (3 94 (9 104 04 1,0 0.000 0000 000 10 (1 [11 [7% 0.034 0034 034 [15 [8% 0.003 0003 003 9 1, 0.00 00 [1 [7 0.03 03 [8 0.0 [ 0.

Early and accurate diagnosis of acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation is crucial for the prognosis of patients. This study identified a potential biomarker for the severity of aGVHD after human leukocyte antigen (HLA)-haploidentical peripheral blood hematopoietic stem cell transplantation (haplo-PBSCT). We included 20 healthy subjects and 57 patients who underwent haplo-PBSCT. Of these patients, 22 developed aGVHD after haplo-PBSCT. The results showed that patients with aGVHD had significantly increased levels of Tim-3+/Perforin+/Granzyme B+CD8+ T cells, but significantly decreased Galectin-9. The differences in Galectin-9 and Tim-3+/Granzyme B+CD8+ T cells between grade I-II aGVHD and III-IV aGVHD were also significant. In vitro, the apoptosis of CD8+ T cells from aGVHD patients was significantly increased after Tim-3/Galectin-9 pathway activation, which decreased Granzyme B secretion. As revealed by univariate analysis, the level of Tim-3+CD8+ T cells was a risk factor for severe aGVHD. ROC analysis demonstrated that high levels of Tim-3+CD8+ T cells had a significant diagnostic value for severe aGVHD, with an area under the curve of 0.854 and cut-off value of 14.155%. In conclusion, the binding of Tim-3 with exogenous Galectin-9 can promote apoptosis of CD8+ T cells and affect the secretion of Granzyme B. Tim-3+CD8+ T cells have the potential to serve as immunological markers for assessing the severity of aGVHD after haplo-PBSCT and identifying patients at a higher risk for severe aGVHD. graftversushost graft versus host (aGVHD HLAhaploidentical HLA haploidentical haploPBSCT. haploPBSCT haplo PBSCT . (haplo-PBSCT) 2 5 PBSCT. Tim3+/Perforin+/Granzyme Tim3PerforinGranzyme TimPerforinGranzyme Tim 3+/Perforin+/Granzyme 3 Perforin BCD8 BCD CD8 CD B+CD8 Galectin9. Galectin9 Galectin 9. 9 Galectin- Tim3+/Granzyme Tim3Granzyme TimGranzyme 3+/Granzyme III I II IIIIV IV vitro Tim3/Galectin9 Tim3Galectin9 TimGalectin 3/Galectin Tim-3/Galectin- activation Tim3+CD8+ Tim3CD8 TimCD 3+CD8+ Tim-3+CD8 0854 0 854 0.85 cutoff cut off 14155 14 155 14.155% conclusion Tim3 Tim- (haplo-PBSCT PerforinGranzyme 3PerforinGranzyme B+CD 3Granzyme Tim3/Galectin Tim3Galectin 3Galectin Tim-3/Galectin Tim3+CD8 Tim3CD 3CD8 3+CD8 Tim-3+CD 085 85 0.8 1415 1 15 14.155 Tim3+CD 3CD 3+CD 08 8 0. 141 14.15 14.1 14.

Abstract Objective: To analyze the value of serum miRNA-122 expression in the diagnosis, severity, and prognosis of Acute Cerebral Infarction (ACI) and the correlation mechanism of serum miRNA-122 on the proliferation and apoptosis of vascular endothelial cells in ACI. Method: A total of 60 patients with ACI who were admitted to the emergency department of the Taizhou People’s Hospital from January 1, 2019, to December 30, 2019, and 30 healthy controls during the same period were selected. General clinical data of all patients at admission were collected. Including age, sex, medical history, and inflammatory factors (C-Reactive Protein [CRP], Interleukin-6 [IL-6], Procalcitonin [PCT], Neutrophil Gelatinase-Associated Lipid carrier protein [NGAL]). The National Institutes of Health Stroke Scale (NIHSS) score at admission and short-term prognosis (the Modified Rankin Score [mRS]) score at 3 months after onset were recorded. The expression level of miRNA-122 in the serum of patients with ACI and normal controls was detected by reverse-transcription quantitative Real-Time Polymerase Chain Reaction (RT-QPCR), and the correlation between the expression level of miRNA-122 in the serum of patients with ACI and the level of inflammatory factors, NIHSS and mRS scores were analyzed. The expression levels of miRNA-122 in the serum of patients with ACI, normal people, and Human Umbilical cord Endothelial Cells (HUVECs) cultured in a blank control group were detected by RT-QPCR and statistically analyzed. MTT and flow cytometry was used to compare the proliferation and apoptosis of vascular endothelial cells in the miRNA-122 mimics and inhibitors transfection groups and the corresponding negative control group. The mRNA and protein levels of apoptosis-related factors Bax, Bcl-2, Caspase-3, and angiogenesis-related proteins Hes1, Notch1, Vascular Endothelial Growth Factors (VEGF), and CCNG1 were detected by RT-QPCR and Western blot. Bioinformatics methods predicted CCNG1 to be the target of miRNA-122, and the direct targeting relationship between CCNG1 and miRNA-122 was verified by a dual-luciferase reporting assay. Result: Serum miRNA-122 expression in patients with ACI was significantly higher than that in healthy controls, with an area under the receiver operating characteristic curve of 0.929, 95% Confidence Interval of 0.875‒0.983, and an optimal cut-off value of 1.397. The expression levels of CRP, IL-6, and NGAL in patients with ACI were higher than those in healthy control groups, p < 0.05; miRNA-122 was positively correlated with CPR, IL-6, NIHSS score, and mRS score. At 48h and 72h, the proliferation rate of HUVECs cells in the miRNA-122 mimics group decreased and the apoptosis rate increased. Cell proliferation rate increased, and apoptosis rate decreased significantly in the groups transfected with miRNA-122 inhibitors. The mRNA and protein levels of pro-apoptotic factors Bax and caspase-3 were significantly increased in the miRNA-122 mimics transfection group, while those of anti-apoptotic factor Bcl-2 were significantly decreased compared to those of the control group. The expression of Bax and Caspase-3 decreased, and the expression of anti-apoptotic factor Bcl-2 increased in the transfected miRNA-122 inhibitors group. mRNA expression levels of Hes1, Notch1, VEGF, and CCNG1 in the miRNA-122 mimic transfected group were significantly decreased, while mRNA expression levels in the miRNA-122 inhibitors transfected group were significantly increased. Bioinformatics showed that there was a miRNA-122 binding site in the 3′UTR region of CCNG1, and dual luciferase assay confirmed that CCNG1 was the target of miRNA-122. Objective miRNA122 miRNA 122 miRNA-12 diagnosis severity (ACI Method 6 Peoples People s 1 2019 selected collected age sex history CReactive C Reactive CRP , [CRP] Interleukin6 Interleukin Interleukin- IL6, IL6 IL [IL-6] PCT, PCT [PCT] GelatinaseAssociated Gelatinase Associated NGAL. . [NGAL]) (NIHSS shortterm short term [mRS] recorded reversetranscription reverse transcription RealTime Real Time RTQPCR, RTQPCR RT QPCR (RT-QPCR) analyzed people (HUVECs apoptosisrelated related Bcl2, Bcl2 Bcl 2, 2 Caspase3, Caspase3 Caspase 3, angiogenesisrelated angiogenesis Hes1 Hes Notch1 Notch VEGF (VEGF) CCNG blot miRNA122, 122, dualluciferase Result 0929 0 929 0.929 95 08750983 875 983 0.875‒0.983 cutoff cut off 1397 397 1.397 6, IL-6 0.05 005 05 CPR h 72h proapoptotic pro apoptotic caspase3 caspase caspase- antiapoptotic anti Bcl- Caspase- 3UTR UTR miRNA122. 122. miRNA12 12 miRNA-1 201 [CRP [IL-6 [PCT [NGAL] [mRS (RT-QPCR (VEGF 092 92 0.92 9 0875098 87 98 0.875‒0.98 139 39 1.39 IL- 0.0 00 miRNA1 miRNA- 20 [IL- [NGAL 09 0.9 087509 8 0.875‒0.9 13 1.3 0. [IL 08750 0.875‒0. 1. 0875 0.875‒0 087 0.875‒ 08 0.875 0.87 0.8

RESUMO Introdução O biatlo é um esporte de neve que combina esqui entre as montanhas e tiro, originário da Escandinávia. Requer que os atletas não só tenham a capacidade de deslizar rapidamente por longas distâncias, mas também que tenham a capacidade de atirar com rapidez e precisão. Existem poucas pesquisas sobre características do biatlo e análises de fatores de influência e estratégias de treinamento na China. Objetivo Este estudo analisa o poder explosivo específico, a resistência e os efeitos do treinamento dos atletas modernos de biatlo nos Jogos Olímpicos de Inverno. Métodos Selecionou-se 20 atletas de biatlo como voluntários de pesquisa. Os indicadores fisiológicos e bioquímicos dos atletas foram testados experimentalmente após o treinamento. Resultados Houve uma correlação entre a velocidade máxima e a altura do teste de duplo suporte (DP) dos atletas (p < 0,05). Os atletas mais pesados esquiam relativamente mais rápido. Porém a taxa excessiva de gordura corporal não é propícia para manter o esqui de alta intensidade a longo prazo. O VO2max dos atletas mostrou-se intimamente relacionado com seu desempenho de esqui e porcentagem de acertos no tiro (p < 0,05). Conclusão A manutenção do treinamento de esqui com armas pode desempenhar um papel positivo na melhoria do nível competitivo dos biatletas de classe mundial. Os músculos do atleta têm uma sólida capacidade de gerar alta potência mecânica em um curto período de tempo. É benéfico aproveitar uma posição favorável após o início da competição.Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction The biathlon is a snow sport that combines cross-country skiing and shooting, originating in Scandinavia. It requires athletes to not only have the ability to glide quickly over long distances but also to have the ability to shoot quickly and accurately. There is little research on biathlon characteristics and analysis of influencing factors and training strategies in China. Objective This study analyzes modern biathlon athletes’ specific explosive power, endurance and training effects at the Winter Olympics. Methods Twenty biathlon athletes were selected as research volunteers. Physiological and biochemical indicators of the athletes were experimentally tested after training. Results There was a correlation between maximum speed and the height of the athletes’ double stand test (SD) (p < 0.05). The heavier athletes skied relatively faster. However, the excessive body fat rate is not conducive to maintaining high-intensity skiing in the long term. The athletes’ VO2max was closely related to their skiing performance and shot hit percentage (p < 0.05). Conclusion Maintaining gun ski training can positively improve the competitive level of world-class biathletes. The athlete's muscles have a solid ability to generate high mechanical power in a short time. It is beneficial to take advantage of a favorable position after the start of the competition. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción El biatlón es un deporte de nieve que combina el esquí entre las montañas y el tiro, originario de Escandinavia. Requiere que los atletas no sólo tengan la capacidad de deslizarse rápidamente por largas distancias, sino también la capacidad de disparar con velocidad y precisión. Hay poca investigación sobre las características del biatlón y el análisis de los factores que influyen y las estrategias de entrenamiento en China. Objetivo Este estudio analiza la potencia explosiva específica, la resistencia y los efectos del entrenamiento de los atletas de biatlón moderno en los Juegos Olímpicos de Invierno. Métodos Se seleccionaron veinte atletas de biatlón como voluntarios para la investigación. Los indicadores fisiológicos y bioquímicos de los atletas fueron comprobados experimentalmente después del entrenamiento. Resultados Hubo una correlación entre la velocidad máxima y la altura de la prueba de doble apoyo (DP) de los atletas (p < 0,05). Los atletas más pesados esquiaron relativamente más rápido. Sin embargo, la excesiva tasa de grasa corporal no es propicia para mantener el esquí de alta intensidad a largo plazo. Se demostró que el VO2max de los atletas estaba estrechamente relacionado con su rendimiento en el esquí y el porcentaje de aciertos en el tiro (p < 0,05). Conclusión El mantenimiento del entrenamiento de esquí de pistola puede desempeñar un papel positivo en la mejora del nivel competitivo de los biatletas de categoría mundial. Los músculos del atleta tienen una sólida capacidad para generar una gran potencia mecánica en un corto período de tiempo. Es beneficioso aprovechar una posición favorable tras el inicio de la competición. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução A escalada em rocha é uma atividade física que exige excelente condicionamento físico. Além de melhorar a aptidão física e a coordenação motora, a escalada requer aperfeiçoamentos constantes no treinamento de habilidade em coordenação muscular para aprimoramento de suas táticas modernas. Objetivo Explorar o treinamento da capacidade de coordenação muscular de escaladores em rocha. Métodos Uma amostragem aleatória composta de 8 atletas foi designada para o experimento envolvendo a mensuração da composição corporal dos atletas, níveis séricos de creatina quinase e ureia sanguínea além de outros biomarcadores coletados antes e após o ensaio. Resultados Houve uma correlação negativa significativa entre o equilíbrio dinâmico e a capacidade de coordenação motora após o treinamento em esportes radicais de escalada em rocha. No segundo dia após o exercício de escalada em rocha, o peso corporal e o conteúdo de gordura dos voluntários mostrou uma redução significativa, e a concentração de creatina quinase (CK) e ureia sanguínea (BU) aumentaram significativamente. Conclusão A exploração do efeito dos esportes radicais na melhoria da capacidade de coordenação muscular dos atletas evidenciou que a força e a coordenação devem ser reforçadas no processo de escalada em rocha. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction Rock climbing is a physical activity that requires excellent physical conditioning. In addition to improving physical fitness and motor coordination, rock climbing requires constant improvements in muscle coordination skill training to enhance its modern tactics. Objective Explore the muscular coordination skill training of rock climbers. Methods A random sample of 8 athletes were assigned to the experiment involving the measurement of athletes’ body composition, serum creatine kinase and blood urea levels, and other biomarkers collected before and after the trial. Results There was a significant negative correlation between dynamic balance and motor coordination ability after training in extreme rock climbing sports. On the second day after the rock climbing exercise, the volunteers’ body weight and fat content significantly reduced, and the concentration of creatine kinase (CK) and blood urea (BU) increased significantly. Conclusion The exploration of extreme sports’ effect on improving athletes’ muscle coordination ability showed that strength and coordination should be enhanced in rock climbing. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción La escalada en roca es una actividad física que exige una excelente condición física. Además de mejorar la forma física y la coordinación motriz, la escalada requiere una mejora constante en el entrenamiento de las habilidades de coordinación muscular para optimizar su táctica moderna. Objetivo Explorar el entrenamiento de las habilidades de coordinación muscular de los escaladores de roca. Métodos Se asignó una muestra aleatoria compuesta por 8 atletas al experimento que incluía la medición de la composición corporal de los atletas, los niveles de creatina quinasa sérica y de urea en sangre, además de otros biomarcadores recogidos antes y después de la prueba. Resultados Se observó una correlación negativa significativa entre el equilibrio dinámico y la capacidad de coordinación motriz tras el entrenamiento en deportes extremos de escalada. El segundo día después del ejercicio de escalada en roca, el peso corporal y el contenido de grasa de los voluntarios mostraron una reducción significativa, y la concentración de creatina quinasa (CK) y de urea en sangre (BU) aumentó significativamente. Conclusión La exploración del efecto de los deportes extremos en la mejora de la capacidad de coordinación muscular de los atletas puso de manifiesto que la fuerza y la coordinación deben mejorarse en el proceso de escalada en roca. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Resumo Antecedentes O impacto de um relato de literatura sobre um determinado assunto pode ser medido pelo seu número de citações. Objetivo O objetivo deste estudo foi avaliar as características dos relatos da literatura na área de tratamento endovascular de aneurismas intracranianos (AIs), analisando os 100 artigos mais citados. Ressalta-se que o foco deste estudo é descrever as características bibliométricas. Métodos Esta análise bibliométrica foi dedicada apenas ao tratamento endovascular dos AIs em nosso estudo. Pesquisamos os 100 artigos mais citados na área de tratamento endovascular de AIs usando a ferramenta de busca daWeb of Science (WOS). Avaliamos as características dessas publicações de alto impacto, incluindo ano de publicação, categoria, periódico, país do autor etc. Resultados Os 100 artigos mais citados foram citados 281,3 vezes, em média. Os Estados Unidos publicaram o maior número de artigos todos os anos em comparação com outros países. Esses artigos altamente citados são publicados principalmente noJournal of Neurosurgery. Oitenta e seis dos 100 artigos mais citados eram estudos clínicos. Conclusão A análise bibliométrica fornece informações sobre o desenvolvimento e a tendência crescente no tratamento endovascular das AI. Este estudo pode ajudar os pesquisadores a entender melhor a visão global desse campo e também fornece pistas sobre áreas promissoras de pesquisas futuras e potenciais colaborações. citações AIs, , (AIs) 10 Ressaltase Ressalta se bibliométricas WOS. WOS . (WOS) publicação categoria periódico etc 2813 281 3 281, vezes média países Neurosurgery clínicos AI colaborações (AIs 1 (WOS 28 2

Abstract Background The impact of a literature report on a particular subject can be measured by its number of citations. Objective The purpose of this study was to evaluate the characteristics of the literature reports in the field of endovascular treatment of intracranial aneurysms (IAs) by analyzing the top 100 most cited articles. It should be noted that the focus of this study is to describe the bibliometric characteristics. Methods This bibliometric analysis dedicated only to endovascular treatment of IAs in our study. We searched the top 100 most cited articles in the field of endovascular treatment of IAs using the search tool of the Web of Science (WOS). We evaluated the characteristics of these high-impact publications, including publication year, category, journal, author's country, etc. Results The top 100 most cited articles were cited 281.3 times, on average. The United States has published the most articles every year compared with other countries. These highly cited articles are mostly published in the Journal of Neurosurgery. Eighty-six of the top 100 most cited articles were clinical studies. Conclusion The bibliometric analysis provides insight over the development and the growing trend in endovascular treatment of IAs. This study can help researchers better understand the global overview of this field, and it also provides leads about promising areas of future research and potential collaborations. citations (IAs 10 WOS. WOS . (WOS) highimpact high publications category journal authors author s country etc 2813 281 3 281. times average countries Neurosurgery Eightysix Eighty six studies collaborations 1 (WOS 28 2

RESUMO Introdução: O treino excessivo no futebol é causado por um desequilíbrio entre a carga corporal, o estresse e a recuperação. O treinamento físico não científico de alto volume e as partidas contínuas de futebol de alta intensidade costumam ser as principais razões do treinamento excessivo dos atletas. Objetivo: Este artigo explora as características das mudanças de função física dos jogadores de futebol durante um ciclo completo de treinamento. Métodos: Usamos métodos experimentais para analisar as mudanças nas características de carga física de jogadores de futebol durante o treinamento de alta intensidade. Resultados: Os indicadores de creatina quinase, nitrogênio da ureia sanguínea e transporte de oxigênio não significativamente durante o treinamento de futebol. A testosterona e o cortisol aumentam gradualmente com o aumento da carga dos exercícios. Conclusões: No treinamento de futebol, precisamos organizar razoavelmente o volume total do exercício, sua intensidade e o tempo de intervalo dos atletas, de acordo com a adaptabilidade física e habilidade atlética do treinador, e com medidas de recuperação nutricional e de entusiasmo. Esses métodos podem melhorar ou aprimorar a função física dos jogadores de futebol. Nível de Evidência II; Estudos terapêuticos - Investigação dos resultados do tratamento.

ABSTRACT Introduction: Overtraining in football is caused by an imbalance between body load, stress, and recovery. High-volume non-scientific physical training and continuous high-intensity football matches are often the main reasons for the overtraining of athletes. Objective: This article explores the characteristics of the changes in physical function of football players during a complete training cycle. Methods: We use experimental methods to analyze the changes in the physical load characteristics of football players during high-intensity training. Results: Creatine kinase, urea nitrogen, and oxygen transport indicators did not change significantly during football training. Testosterone and cortisol will gradually increase with an increase of exercise load. Conclusion: In football training, we need to reasonably arrange the total exercise volume, exercise intensity, and exercise interval time of the athletes according to the trainer’s physical adaptability and athletic ability, supplemented with nutrition and enthusiasm recovery measures. These methods can improve or enhance the physical function of football players. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Introducción: El sobreentrenamiento en el fútbol es causado por un desequilibrio entre la carga corporal, el estrés y la recuperación. El entrenamiento físico de alto volumen no científico y los partidos de fútbol continuos de alta intensidad suelen ser las principales razones del sobreentrenamiento de los atletas. Objetivo: Este artículo explora las características de los cambios en la función física de los jugadores de fútbol durante un ciclo completo de entrenamiento. Métodos: Utilizamos métodos experimentales para analizar los cambios en las características de la carga física de los jugadores de fútbol durante el entrenamiento de alta intensidad. Resultados: Los indicadores de creatina quinasa, nitrógeno ureico en la sangre y transporte de oxígeno no fueron significativos durante el entrenamiento. La testosterona y el cortisol aumentan gradualmente con el aumento de la carga de ejercicios. Conclusiones: En el entrenamiento de fútbol, se debe organizar razonablemente el volumen total de ejercicios, la intensidad y el tiempo de intervalo de los atletas en función del acondicionamiento físico y la capacidad atlética del entrenador, y con medidas de recuperación nutricional y de entusiasmo. Estos métodos pueden mejorar o potenciar la función física de los jugadores de fútbol. Nivel de Evidencia II; Estudios terapéuticos - Investigación de los resultados del tratamiento.

ABSTRACT BACKGROUND: Computer-aided diagnosis in low-dose (≤ 3 mSv) computed tomography (CT) is a potential screening tool for lung nodules, with quality interpretation and less inter-observer variability among readers. Therefore, we aimed to determine the screening potential of CT using a radiation dose that does not exceed 2 mSv. OBJECTIVE: We aimed to compare the diagnostic parameters of low-dose (< 2 mSv) CT interpretation results using a computer-aided diagnosis system for lung cancer screening with those of a conventional reading system used by radiologists. DESIGN AND SETTING: We conducted a comparative study of chest CT images for lung cancer screening at three private institutions. METHODS: A database of low-dose (< 2 mSv) chest CT images of patients at risk of lung cancer was viewed with the conventional reading system (301 patients and 226 nodules) or computer-aided diagnosis system without any subsequent radiologist review (944 patients and 1,048 nodules). RESULTS: The numbers of detected and solid nodules per patient (both P < 0.0001) were higher using the computer-aided diagnosis system than those using the conventional reading system. The nodule size was reported as the maximum size in any plane in the computer-aided diagnosis system. Higher numbers of patients (102 [11%] versus 20 [7%], P = 0.0345) and nodules (154 [15%] versus 17 [8%], P = 0.0035) were diagnosed with cancer using the computer-aided diagnosis system. CONCLUSIONS: The computer-aided diagnosis system facilitates the diagnosis of cancerous nodules, especially solid nodules, in low-dose (< 2 mSv) CT among patients at risk for lung cancer.

Resumo Introdução A rinite alérgica é uma forma de inflamação da mucosa nasal mediada por IgE em resposta a alérgenos específicos, resulta em sintomas típicos. Objetivos Comparar a eficácia clínica da neurectomia nasal posterior com ou sem neurectomia faríngea para o tratamento da rinite alérgica perene de moderada a grave. Além disso, comparar a gravidade das comorbidades, inclusive tosse crônica e asma, entre os pacientes nesses dois grupos de tratamento cirúrgico. Método Foram incluidos neste ensaio clínico randomizado e designados para o grupo controle (neurectomia nasal posterior) ou para o grupo experimental (neurectomia nasal posterior + neurectomia faríngea) 52 pacientes. A escala visual analógica e o questionário de qualidade de vida na rinoconjuntivite (rhinoconjunctivitis quality of life questionnaire) foram usados para comparar as diferenças nos sintomas dos pacientes entre o período inicial e 6, 12 e 24 meses após o tratamento. Além disso, a tosse e os sintomas de asma dos pacientes foram monitorados durante o acompanhamento por meio da escala visual analógica e do teste de controle da asma (asthma control test ), respectivamente. Resultados Nenhuma diferença significante nos escores pré‐operatórios foi evidenciada entre os grupos (p > 0,05). Aos seis meses pós‐tratamento, houve diferenças significantes nos escores da escala visual analógica, no questionário de qualidade de vida na rinoconjuntivite e no teste de controle de asma em relação aos valores basais dos pacientes no grupo experimental e no grupo controle (p < 0,05), o que permaneceu verdadeiro após 12 e 24 meses de acompanhamento. Não foram observadas diferenças significantes nos escores da escala visual analógica e nem no questionário de qualidade de vida para conjuntivite ou no teste de controle da asma entre os dois grupos de tratamento em qualquer momento do acompanhamento pós‐operatório (p > 0,05), enquanto a gravidade da tosse foi significantemente reduzida no grupo experimental em relação ao grupo controle (p < 0,05). Conclusão A neurectomia nasal posterior pôde ser feita com segurança com ou sem neurectomia faríngea para o tratamento eficaz da rinite alérgica. O tratamento combinado com neurectomia nasal posterior e neurectomia faríngea pode oferecer mais benefício do que a neurectomia nasal posterior isolada para o tratamento de pacientes com rinite alérgica e tosse crônica. específicos típicos grave disso comorbidades cirúrgico 5 rhinoconjunctivitis questionnaire 6 1 2 asthma , ) respectivamente préoperatórios pré operatórios p 0,05. 005 0,05 . 0 05 0,05) póstratamento pós pós‐tratamento 0,05, pósoperatório operatório 00 0,0 0,

Abstract Introduction Allergic rhinitis is a form of IgE mediated inflammation of the nasal mucosa in response to specific allergens, resulting in typical symptoms. Objectives This study was designed with the primary goal of comparing the clinical efficacy of posterior nasal neurectomy with or without pharyngeal neurectomy for the treatment of moderate-to-severe perennial allergic rhinitis. Secondary study aims included a comparison of the severity of comorbidities, including chronic cough and asthma, between patients in these two surgical treatment groups. Methods A total of 52 patients were enrolled in this randomized controlled trial and were assigned to either the control group (posterior nasal neurectomy) or the experimental group (posterior nasal neurectomy + pharyngeal neurectomy). The visual analog scale and rhinoconjunctivitis quality of life questionnaire were used to compare the differences in patient symptoms between baseline and 6-, 12-, and 24-months post-treatment. In addition, patient cough and asthma symptoms were monitored during follow-up via visual analog scale and asthma control test respectively. Results No significant differences in preoperative scores were evident between groups (p> 0.05). At 6-months post-treatment, there were significant differences in visual analog scale, rhinoconjunctivitis quality of life questionnaire, and asthma control test scores relative to baseline values in experimental group and control group patients (p< 0.05), and this remained true upon 12- and 24-month follow-up. No significant differences in visual analog scale, rhinoconjunctivitis quality of life questionnaire, or asthma control test scores were observed between the two treatment groups at any postoperative follow-up time point (p> 0.05), while coughing severity was found to be significantly reduced in the experimental group relative to the control group (p< 0.05). Conclusion posterior nasal neurectomy can be safely implemented with or without pharyngeal neurectomy in order to effectively treat allergic rhinitis. Combined posterior nasal neurectomy and pharyngeal neurectomy treatment may offer greater value than posterior nasal neurectomy alone for the treatment of allergic rhinitis patients with chronic cough. allergens moderatetosevere moderate severe comorbidities 5 neurectomy. . 6, 6 , 6- 12, 12 24months months 24 posttreatment. posttreatment post treatment. post-treatment addition followup follow up respectively p> p (p 0.05. 005 0.05 0 05 0.05) 6months posttreatment, treatment, p< 0.05, 24month month followup. up. 1 2 00 0.0 0.

Resumo: Governança significa a política e a estratégia adotadas no processo de gestão de um país. Na longa história de mais de dois mil anos, o confucionismo tornou-se o núcleo cultural e a doutrina política da autocracia imperial, e a teoria da “governança de todo o país” contida nele tornou-se uma categoria importante na cultura tradicional chinesa. Deve-se aprofundar na rica conotação do pensamento de governança confucionista e construir um sistema de governança nacional que se adapte à sociedade, à situação geral e ao futuro, por meio de desenvolvimento inovador e transformação criativa, de modo a permitir que a excelente cultura tradicional chinesa retorne à base, para abrir um novo caminho e fazer o passado servir ao presente, fornecendo, assim, importante orientação teórica e referência histórica para o grande rejuvenescimento da nação chinesa.

Abstract: Governance means the policy and strategy adopted in the process of running a country. In the long history of more than two thousand years, Confucianism had become the cultural core and political doctrine of the imperial autocracy, and the theory of “governance of the whole country” contained in it had become an important category in traditional Chinese culture. We should dig deep into the rich connotation of Confucian governance thought, and build a national governance system that suits society, the overall situation and future through innovative development and creative transformation, so as to let excellent traditional Chinese culture return to open a new road and make the past serve the present, thus providing important theoretical guidance and historical reference for the great rejuvenation of the Chinese nation.

Abstract Serrapeptase, a proteolytic enzyme, has been used for the adjuvant treatment of many diseases. However, its fibrinolytic activity is still uncertain. Herein, the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects were investigated. The results showed that the fibrinolytic activity of serrapeptase was 1295 U/mg, and the specific activity was 3867 U/mg of protein when its proteolytic activity toward casein was 2800 U/mg. The optimum temperature and pH for serrapeptase activity were 37-40°C and 9.0, respectively. At 1 mmol/L, Zn2+, Mn2+ and Fe2+ could activate the fibrinolytic activity of serrapeptase, while K+, Cu2+, sodium dodecyl sulfate (SDS) and ethylene diamine tetraacetic acid (EDTA) inhibited it. In vitro tests showed that serrapeptase could completely prevent blood coagulation at 150 U/mL, and the percentage of blood clot lysis reached 96.6% at 37°C after 4 h at 300 U/mL. These results indicate that serrapeptase has excellent fibrinolytic activity, and can be used as a health food or candidate drug for the prevention or treatment of thrombotic diseases.