RESUMO: Este estudo explorou o uso exclusivo do ultrassom (US) para extrair proteínas das espinhas dorsais da carpa-capim (Ctenopharyngodon idella) em baixas temperaturas e examinou seu impacto na hidrofobicidade, solubilidade, perfil eletroforético e níveis de sulfidrilas. As espinhas dorsais foram tratadas com 35 e 130 kHz por 20, 30 e 40 minutos a 14 ºC, resultando em duas frações de proteínas: sólida (TPS) e líquida (TPL). O US aumentou o rendimento em comparação com a fração não sonificada. TPL (35 kHz) apresentou uma redução de 16% nos níveis totais e livres de sulfidrila e um aumento de 25% na hidrofobicidade. O US induziu alterações na conformação proteica e na intensidade das bandas, especialmente entre 25 e 100 kDa para TPL a 130 kHz e abaixo de 30 kDa para TPS a 35 kHz. Este estudo demonstrou a eficácia do US na extração e na modificação de algumas propriedades das proteínas de subprodutos de peixe, facilitando assim seu emprego no desenvolvimento de produtos alimentícios inovadores. RESUMO (US carpacapim carpa capim Ctenopharyngodon idella hidrofobicidade solubilidade sulfidrilas 3 13 20 4 1 ºC (TPS TPL. . (TPL) sonificada (3 16 bandas 2 10 peixe inovadores (TPL (

ABSTRACT: This study explored the use only of ultrasound (US) for extracting proteins from grass carp (Ctenopharyngodon idella) backbones at low temperature and examined its impact on hydrophobicity, solubility, electrophoretic profile, and sulfhydryl levels. Backbones were treated with 35 and 130 kHz for 20, 30, and 40 minutes at 14 ºC, resulting in two protein fractions: solid (TPS) and liquid (TPL). US increased yield compared to the non-sonicated fraction. TPL (35 kHz) exhibited a 16% reduction in total and free sulfhydryl levels and a 25% increase in hydrophobicity. US induced protein conformation and band intensity alterations, particularly between 25 to 100 kDa for TPL at 130 kHz and below 30 kDa for TPS at 35 kHz. This study demonstrated the efficacy of US for protein extraction from fish by-products and its capacity to modify protein characteristics, facilitating the development of innovative food products. ABSTRACT (US Ctenopharyngodon idella hydrophobicity solubility profile 3 13 20 4 1 ºC fractions (TPS TPL. . (TPL) nonsonicated non sonicated fraction (3 16 alterations 2 10 byproducts by products characteristics (TPL (

Abstract The main objective of this study was to evaluate the effects of supplementation the diet of pigs with grape pomace preserved in silage form (GPS) and its interaction with indoor and outdoor production systems, with and without access to vegetation, on the attributes of meat quality produced. Analyzes of proximal composition, cholesterol content, fatty acid profile, shear force, texture profile and sensory analysis were performed. During cold storage, oxidative stability and objective color were analyzed. Statistical analysis was performed in a 3x2 factorial design (production systems (S) x GPS-feed (F)) and the interaction between them (S*F). The results showed that there was no interaction between the production system and GPS feeding for the attributes evaluated. The proximate composition and fatty acid profile of the muscle remained unchanged. Additionally, it provides higher subjective and objective tenderness, higher red color intensity, and reduces lipid oxidation under refrigeration. The supplementation of pig feed with GPS improve the quality of the meat and constitute a sustainable alternative for the winemaking residue. (GPS vegetation produced content force storage analyzed 3x S (S GPSfeed F (F) S*F. SF S*F . (S*F) evaluated unchanged Additionally tenderness intensity refrigeration residue (F (S*F

Abstract Gluten-free products and non-alcoholic beverages have become popular global trends. This study assessed strategies for producing low-alcoholic and low or gluten-free beers. Barley malts were fermented by Saccharomyces cerevisiae or Saccharomycodes ludwigii, and commercial endopeptidase was used to reduce gluten. S. cerevisiae is the most used yeast in alcoholic beer, so vacuum evaporation was used to reduce values of ethanol. S. ludwigii produces less alcohol, however, there was no data reported it would ferment on a semi-industrial scale. The physiochemical parameters in rice malt beers were similar to both yeasts. However, the parameters found differed for beer with barley malt mainly ethanol values, confirming the fermentative difference by tested strains. Volatile composition was evaluated and analyzed by multivariate analysis. The beers proposed by different methodologies: S. cerevisiae with barley malt, peptidase, and evaporation exhibited aromas of floral and featuring beer and spice notes, among others; S. ludwigii fermentation with barley malt and peptidase showcased aromas rich in floral and fresh accents, among others; and, S. cerevisiae with malted rice exhibited notes evoking fruity notes reminiscent of roses and S. ludwigii using malted rice was associated with aromas featuring fruity attributes and emitting rancid notes, among others. Among all the strategies tested, the one using barley malt, enzyme, and S. cerevisiae proved results in terms of aromatic parameters, even with the final boiling process to remove the alcohol. Glutenfree Gluten free nonalcoholic non trends lowalcoholic glutenfree gluten S alcohol however semiindustrial semi industrial scale yeasts However strains analysis methodologies others accents enzyme

Abstract This work developed biscuits with the partial substitution of wheat flour with olive pomace flour (OPF). The wheat flour contents substituted by OPF in the biscuits were 0%, 10%, and 20%. The effects of the flour on the chemical composition, lipid profile, and sensory aspects of the reformulated biscuits were evaluated. The data showed that the OPF has a high concentration of dietary fiber (43.75%) and 27.70% lipids. The biscuit with 10% of the wheat flour replaced by OPF has 4.29% of total dietary fiber and 22.61% of lipids, whereas the biscuits made by substituting 20% of the wheat flour with OPF have 8.52% of total dietary fiber and 23.78% of lipids. The incorporation of OPF increased the contents of lipids, minerals, and dietary fiber. Sensorially, the sensory descriptors were not beneficial to characterize the biscuits reformulated with OPF. Also, the lightness, texture, and brittleness decreased in the biscuits with added OPF, as did the loss from baking. Hence, the BIS10 biscuit proved to have the potential to be better explored from the physicochemical and sensory viewpoint. . (OPF) 0 0% 10 20 composition profile evaluated 43.75% 4375 43 75 (43.75% 2770 27 70 27.70 lipids 429 4 29 4.29 2261 22 61 22.61 852 8 52 8.52 2378 23 78 23.78 minerals Sensorially Also lightness texture baking Hence BIS BIS1 viewpoint (OPF 1 2 43.75 437 7 (43.75 277 27.7 42 4.2 226 6 22.6 85 5 8.5 237 23.7 43.7 (43.7 27. 4. 22. 8. 23. 43. (43. (43 (4 (

Resumo Este artigo discute as práticas da psicanálise em equipes de saúde mental pública, investigando o modo como psicanalistas atuam fora do modelo ambulatorial individualizado. A pesquisa foi realizada a partir de uma perspectiva etnográfica, por meio do acompanhamento da rotina de trabalho de dois psicanalistas em uma equipe de saúde mental. O registro detalhado das rotinas em diário de campo foi utilizado como material de análise. Em decorrência disso, propõe-se o modelo do tear psicanalítico para ilustrar o modo como a psicanálise opera em interlocução com os demais saberes que compõem a saúde mental pública.

Abstract This article discusses the psychoanalysis practices employed by public mental health care teams, investigating how psychoanalysts act outside the individualized ambulatory model. An ethnographic research was carried out by monitoring the work routines of two psychoanalysts from a mental health care team. Detailed recordings of the routines in the field diary were used as material for analysis. Hence, this essay proposes the psychoanalytic loom approach to illustrate how psychoanalysis operates in dialogue with other knowledge that make up public mental health.

Resumen Este artículo discute las prácticas del psicoanálisis en equipos de salud mental pública, investigando el modo de actuar de los psicoanalistas fuera del modelo ambulatorio individualizado. La investigación se realizó a partir de la perspectiva etnográfica por medio del seguimiento de la rutina de trabajo de dos psicoanalistas en un equipo de salud mental. El registro detallado de las rutinas en diario de campo se utilizó como material de análisis. Con eso, se propone el modelo del telar psicoanalítico como ilustración del modo como se opera el psicoanálisis en interlocución con los demás saberes que componen la salud mental pública.

Résumé Cet article traite des pratiques de psychanalyse utilisées par les équipes publiques de santé mentale, en examinant comment les psychanalystes agissent en dehors du modèle ambulatoire individualisé. Une recherche ethnographique a été menée en suivant les routines de travail de deux psychanalystes d’une équipe de santé mentale. Les enregistrements détaillant des routines dans le journal de bord ont été utilisé comme matériel d’analyse. Cet essai propose donc l’approche du métier à tisser psychanalytique pour illustrer comment la psychanalyse opère en interaction avec les autres savoirs qui composent la santé mentale publique.

Abstract This study tested the hypothesis that head and neck radiotherapy (HNRT) impacts the immunoexpression of type I collagen, bone sialoprotein (BSP) and bone morphogenetic protein 4 (BMP4), thereby leading to micromorphological changes in the dentin-pulp complex (DPC), and promoting the onset and progression of radiation caries (RC). Twenty-two demineralized sections of carious teeth (a group of 11 irradiated teeth and a control group of 11 non-irradiated teeth) extracted from 19 head and neck cancer patients were analyzed by conventional optical microscopy and immunohistochemistry to investigate the micromorphology (cellular layer hierarchy, blood vessels, odontoblasts, fibroblasts, extracellular matrix, calcification, necrosis, reactionary dentin formation, and chronic inflammation), and the patterns of staining/immunolocalization of type I collagen, BSP and BMP4 in the dental pulp of irradiated and control samples. No significant differences attributable to the direct impact of radiotherapy were detected in DPC micromorphology between the groups. In addition, the patterns of immunohistochemical staining and immunolocalization of the proteins studied did not differ between the irradiated and the control samples for type I collagen, BSP or BMP4. This study rejected the hypothesis that HNRT directly damages dentition by changing the organic components and the microstructure of the DPC, ultimately leading to RC.

Resumo Objetivo: Analisar a rede de correlações entre as dimensões do Modelo Desequilíbrio Esforço-Recompensa (DER), resiliência e qualidade de vida em policiais militares. Método: Estudo transversal realizado com 258 policiais do Batalhão de Operações Especiais (BOE) da Polícia Militar de uma cidade do Rio Grande do Sul. As escalas do Modelo DER, de resiliência e World Health Quality of Life (WHOQOL-bref) avaliaram o estresse psicossocial, resiliência e qualidade de vida. Foram realizadas estatísticas descritiva e analítica, com análise de rede. Resultados: As dimensões do Modelo DER e a resiliência se correlacionaram com todos os domínios do WHOQOL (p<0,001). O modelo de rede indicou associação negativa entre o esforço, o excesso de comprometimento e os domínios físico e meio ambiente. O domínio meio ambiente foi associado positivamente à recompensa, e a resiliência associou-se positivamente com os domínios geral, físico e psicológico. Conclusão: Concluiu-se que o estresse psicossocial interfere na qualidade de vida dos policiais militares e a resiliência pode atuar como um fator de proteção.

Abstract Objective: To analyze the network of correlations between the realms of the Effor t-Reward Imbalance (ERI) model, resilience, and quality of life among military police officers. Method: This is a cross-sectional study conducted with 258 police officers from the Special Opera tions Battalion of the Military Police in a city in Rio Grande do Sul, Brazil. The scales of the ERI model, resilience, and World Health Quality of Life (WHOQOL-bref) assessed psychosocial stress, resilience, and quality of life. Descriptive and analytical statistics were performed em ploying the network analysis. Results: The realms of the ERI model and resilience correlated with all the WHOQOL’s realms (p<0.001). The network model showed a negative association between effort, overcommitment, and physical and environmental realms. The environmental realm was positively associated with reward, while resilience was positively associated with the general, physical, and psychological realms. Conclusion: We concluded that psychosocial stress interferes in the quality of life of military police officers, and resi lience may work as a protective factor.

Resumo Objetivo: Analisar a rede de correlações entre as dimensões do Modelo Desequilíbrio Esforço-Recompensa (DER), resiliência e qualidade de vida em policiais militares. Método: Estudo transversal realizado com 258 policiais do Batalhão de Operações Especiais (BOE) da Polícia Militar de uma cidade do Rio Grande do Sul. As escalas do Modelo DER, de resiliência e World Health Quality of Life (WHOQOL-bref) avaliaram o estresse psicossocial, resiliência e qualidade de vida. Foram realizadas estatísticas descritiva e analítica, com análise de rede. Resultados: As dimensões do Modelo DER e a resiliência se correlacionaram com todos os domínios do WHOQOL (p<0,001). O modelo de rede indicou associação negativa entre o esforço, o excesso de comprometimento e os domínios físico e meio ambiente. O domínio meio ambiente foi associado positivamente à recompensa, e a resiliência associou-se positivamente com os domínios geral, físico e psicológico. Conclusão: Concluiu-se que o estresse psicossocial interfere na qualidade de vida dos policiais militares e a resiliência pode atuar como um fator de proteção.

Abstract Objective: To analyze the network of correlations between the realms of the Effor t-Reward Imbalance (ERI) model, resilience, and quality of life among military police officers. Method: This is a cross-sectional study conducted with 258 police officers from the Special Opera tions Battalion of the Military Police in a city in Rio Grande do Sul, Brazil. The scales of the ERI model, resilience, and World Health Quality of Life (WHOQOL-bref) assessed psychosocial stress, resilience, and quality of life. Descriptive and analytical statistics were performed em ploying the network analysis. Results: The realms of the ERI model and resilience correlated with all the WHOQOL’s realms (p<0.001). The network model showed a negative association between effort, overcommitment, and physical and environmental realms. The environmental realm was positively associated with reward, while resilience was positively associated with the general, physical, and psychological realms. Conclusion: We concluded that psychosocial stress interferes in the quality of life of military police officers, and resi lience may work as a protective factor.

Abstract The objective of this study was to evaluate whether the addition of a blend based on α-monolaurin mono-, di- and triglycerides of butyric acid, and lysolecithin on the performance even on diets containing reduced inclusion of oil in the diet and without the use of growth-promoting antibiotics of broilers considering the effect on health, performance, and meat. Three treatments were defined: positive control (TP: with enramycin), negative control (TN: no enramycin), and blend (T-FRA: with monolaurin and glycerides of acid butyric minus 0.8% soybean oil). At 21 days, broilers treated with TP and T-FRA obtained the lower feed conversion ratio (FC); at 35 days, T-FRA broilers obtained lower FC than TN broilers. Cholesterol levels were higher in the blood of T-FRA broilers. On day 42, levels of ROS and TBARS were lower in the intestine, muscles, and liver of T-FRA broilers. Moreover, glutathione S-transferase and total non-enzymatic antioxidants were greater at the intestinal and muscular levels. The T-FRA broilers had a lower percentage of lipids in the meat. The MIC indicated that 111mg of the blend/mL inhibited the growth of E. coli; however, the counts of total coliforms and E. coli in the feces and the broilers’ litter did not differ between treatments. In conclusion, the addition of the blend T-FRA in broiler diets was able to improve the feed conversion and maintain the other performance parameters even considering a reduction of 0.8% in the inclusion of oil.

The use of paper devices in chemical analysis has been increased due to their low cost, high surface area, portability, and biodegradability. A novel use of paper devices is proposed using thermal infrared enthalpimetry (TIE) for a rapid, green, and high throughput determination of wine acidities. The determination of total, fixed, and volatile acidities in red, white, and sparkling wine was carried out, and the results were compared with AOAC titration method. A multichannel pipette was used for reagent addition and an infrared camera for the temperature monitoring. The pipetting and temperature measurement conditions presented direct influence in the deviations among the measurements. The results obtained by TIE presented an agreement between 96 and 104% with the reference method (AOAC 962.12), for all samples. A high sample throughput was achieved reaching 480 samples h-1 for total acidity. A significant reduction in sample volume and reagent consumption was also obtained, with energy consumption up to 60 times lower in relation to the AOAC method. The association of TIE with a paper device provided a rapid, reliable and green method for the determination of acidities of wines.

Abstract In this work, a simple and fast method is proposed for the determination of ethanol in homeopathic medicines using thermal infrared enthalpimetry (TIE). Samples containing alcohol in a wide concentration range (from 5% to 95% v/v) were used. Purified water or absolute ethanol was added directly into homeopathic medicine and increase of temperature was monitored using an infrared camera. Total volume, stirring speed and dispensing rate of solutions were the most significant parameters studied for method optimization. A response surface methodology (RSM) was used for optimization of the experimental conditions. The method was validated in the following parameters: selectivity, linearity, linear range, precision (repeatability and intermediate precision), limit of detection and quantification, accuracy and robustness. Linear range was obtained from 4% to 55% (ethanol, v/v). The proposed method showed accuracy in agreement with the conventional one. The proposed method it was demonstrated a good alternative for determination of ethanol in homeopathic medicines, presenting low cost, fast analysis and agreement with the principles of green analytical chemistry.

Abstract Inflammatory dermatoses are prevalent worldwide, with impacts on the quality of life of patients and their families. The aim of this study was to determine the anti-inflammatory effects of Achyrocline satureioides oily extracts and nanocapsules on the skin using a mouse model of irritant contact dermatitis induced by croton oil, and a skin inflammation model induced by ultraviolet B (UVB) radiation. The mice were treated with 15 mg/ear oily extract (HG-OLAS) or nanocapsules (HG-NCAS) of A. satureioides incorporated into Carbopol® 940 hydrogels. We found that HG-OLAS and HG-NCAS formulations reduced ear edema in croton oil-induced lesions with maximum inhibitions of 54±7% and 74±3%, respectively. HG-OLAS and HG-NCAS formulations decreased ear edema induced by UVB radiation (0.5 J/cm2), with maximum inhibitions of 68±6% and 76±2% compared to the UVB radiation group, respectively. HG-OLAS and HG-NCAS modulated myeloperoxidase (MPO) activity after croton oil induction. Furthermore, croton oil and UVB radiation for 6 and 24 h, respectively, stimulated polymorphonuclear cells infiltration. The topical treatments reduced inflammatory processes, as shown by histological analysis. Together, the data suggest that topical application of A. satureioides oily extracts and nanocapsules produced antiedematogenic and anti-inflammatory effects. They constitute a compelling alternative for treatment of skin injuries.

Abstract Abstract: This study evaluated the effect of oregano essential oil added to the feed of commercial laying hens. This research was focused on the analysis of biochemical changes linked to hepatic function, and protein and lipid metabolism. It was used 240 laying hens (59 weeks-old) distributed in a completely randomized design of six treatments (five repetitions with eight birds each). The experiments were constituted by a control treatment (CT) with the inclusion of zinc bacitracin and five treatments of oregano essential oil (OEO: 0, 50, 100, 150, and 200 mg.kg-1), respectively. After 28 days of feeding, an increase on serum levels of total proteins and globulins was observed on groups T150 and T200, as well as an increase on albumin levels on group CT. After 84 days of feeding, a significant reduction on total proteins and albumin was observed on group T200, as well as an increase in serum triglycerides. OEO at 200 mg.kg-1 increased globulin levels on day 28, which may be considered an effect in the inflammatory response, which increases serum immunoglobulins and proteins.

RESUMO: O objetivo desse estudo foi investigar a influência da adição dos prebióticos farelo de arroz, inulina e hi-maize, na sobrevivência de Lactobacillus acidophilus em micropartículas de alginato obtidas por gelificação externa seguida de liofilização. Analisou-se o tamanho das micropartículas, a viabilidade, em simulação gastrointestinal e estabilidade, durante armazenamento. O tamanho das micropartículas variou de 127.5µm a 234.6µm. As micropartículas adicionadas dos diferentes prebióticos demonstraram um aumento na proteção do microrganismo, que apresentou maior viabilidade frente à simulação gastrointestinal. Quanto ao armazenamento em diferentes condições, a 25ºC o tratamento farelo de arroz manteve os probióticos viáveis por 30 dias. Nas condições de armazenamento -18ºC e 7°C os tratamentos contendo os prebióticoshi-maize e farelo de arroz mantiveram os microrganismos probióticos viáveis por um período de 60 dias.

ABSTRACT: The aim of this study was to investigate the influence of the addition of prebiotics rice bran, inulin and hi-maize, on the survival of Lactobacillus acidophilus in alginate microparticles obtained by external ionic gelation followed by freeze-drying. The microparticles size ranged from 127.5μm to 234.6μm. Microparticles added from the different prebiotics demonstrated an increase in the protection of the microorganism, which presented greater viability against the gastrointestinal simulation. As for storage under different conditions, rice bran treatment at 25ºC kept probiotics viable for 30 days. Under storage conditions -18°C and 7°C, treatments containing prebiotics hi-maize and rice bran maintained viable probiotic microorganisms for a period of 60 days.

Abstract One hundred fifty fungal strains were isolated from the Pampa biome for production of polyunsaturated fatty acids (PUFA). Microbial oil was produced by submerged cultivation at 120 rpm, 28 ºC for 7 days. Seven strains showed the best results for lipids accumulation, and they were selected in order to evaluate the influence of the temperature (15 and 28 ºC) on the lipid production and profile. The fungus Nigrospora sp. produced the highest lipid level: at 28 ºC were produced 11.28% of lipids and 51.94% of PUFA and at 15 ºC were produced 6.02% of lipids and 42.39% of PUFA. Plackett Burman design was performed to investigate the main effects of the temperature (15-25 ºC), pH (4.0-6.0), agitation (120-200 rpm), corn steep liquor (5-15% (v/v)) and sucrose concentrations (60-180 g L-1). The highest PUFA production (55.75%) was observed at the lowest temperatures (15 ºC), whereas the maximum lipid production was 5.54% at 25 ºC.