Abstract The main objective of this study was to evaluate the effects of supplementation the diet of pigs with grape pomace preserved in silage form (GPS) and its interaction with indoor and outdoor production systems, with and without access to vegetation, on the attributes of meat quality produced. Analyzes of proximal composition, cholesterol content, fatty acid profile, shear force, texture profile and sensory analysis were performed. During cold storage, oxidative stability and objective color were analyzed. Statistical analysis was performed in a 3x2 factorial design (production systems (S) x GPS-feed (F)) and the interaction between them (S*F). The results showed that there was no interaction between the production system and GPS feeding for the attributes evaluated. The proximate composition and fatty acid profile of the muscle remained unchanged. Additionally, it provides higher subjective and objective tenderness, higher red color intensity, and reduces lipid oxidation under refrigeration. The supplementation of pig feed with GPS improve the quality of the meat and constitute a sustainable alternative for the winemaking residue. (GPS vegetation produced content force storage analyzed 3x S (S GPSfeed F (F) S*F. SF S*F . (S*F) evaluated unchanged Additionally tenderness intensity refrigeration residue (F (S*F

Abstract This work developed biscuits with the partial substitution of wheat flour with olive pomace flour (OPF). The wheat flour contents substituted by OPF in the biscuits were 0%, 10%, and 20%. The effects of the flour on the chemical composition, lipid profile, and sensory aspects of the reformulated biscuits were evaluated. The data showed that the OPF has a high concentration of dietary fiber (43.75%) and 27.70% lipids. The biscuit with 10% of the wheat flour replaced by OPF has 4.29% of total dietary fiber and 22.61% of lipids, whereas the biscuits made by substituting 20% of the wheat flour with OPF have 8.52% of total dietary fiber and 23.78% of lipids. The incorporation of OPF increased the contents of lipids, minerals, and dietary fiber. Sensorially, the sensory descriptors were not beneficial to characterize the biscuits reformulated with OPF. Also, the lightness, texture, and brittleness decreased in the biscuits with added OPF, as did the loss from baking. Hence, the BIS10 biscuit proved to have the potential to be better explored from the physicochemical and sensory viewpoint. . (OPF) 0 0% 10 20 composition profile evaluated 43.75% 4375 43 75 (43.75% 2770 27 70 27.70 lipids 429 4 29 4.29 2261 22 61 22.61 852 8 52 8.52 2378 23 78 23.78 minerals Sensorially Also lightness texture baking Hence BIS BIS1 viewpoint (OPF 1 2 43.75 437 7 (43.75 277 27.7 42 4.2 226 6 22.6 85 5 8.5 237 23.7 43.7 (43.7 27. 4. 22. 8. 23. 43. (43. (43 (4 (

Resumo Este artigo discute as práticas da psicanálise em equipes de saúde mental pública, investigando o modo como psicanalistas atuam fora do modelo ambulatorial individualizado. A pesquisa foi realizada a partir de uma perspectiva etnográfica, por meio do acompanhamento da rotina de trabalho de dois psicanalistas em uma equipe de saúde mental. O registro detalhado das rotinas em diário de campo foi utilizado como material de análise. Em decorrência disso, propõe-se o modelo do tear psicanalítico para ilustrar o modo como a psicanálise opera em interlocução com os demais saberes que compõem a saúde mental pública.

Abstract This article discusses the psychoanalysis practices employed by public mental health care teams, investigating how psychoanalysts act outside the individualized ambulatory model. An ethnographic research was carried out by monitoring the work routines of two psychoanalysts from a mental health care team. Detailed recordings of the routines in the field diary were used as material for analysis. Hence, this essay proposes the psychoanalytic loom approach to illustrate how psychoanalysis operates in dialogue with other knowledge that make up public mental health.

Resumen Este artículo discute las prácticas del psicoanálisis en equipos de salud mental pública, investigando el modo de actuar de los psicoanalistas fuera del modelo ambulatorio individualizado. La investigación se realizó a partir de la perspectiva etnográfica por medio del seguimiento de la rutina de trabajo de dos psicoanalistas en un equipo de salud mental. El registro detallado de las rutinas en diario de campo se utilizó como material de análisis. Con eso, se propone el modelo del telar psicoanalítico como ilustración del modo como se opera el psicoanálisis en interlocución con los demás saberes que componen la salud mental pública.

Résumé Cet article traite des pratiques de psychanalyse utilisées par les équipes publiques de santé mentale, en examinant comment les psychanalystes agissent en dehors du modèle ambulatoire individualisé. Une recherche ethnographique a été menée en suivant les routines de travail de deux psychanalystes d’une équipe de santé mentale. Les enregistrements détaillant des routines dans le journal de bord ont été utilisé comme matériel d’analyse. Cet essai propose donc l’approche du métier à tisser psychanalytique pour illustrer comment la psychanalyse opère en interaction avec les autres savoirs qui composent la santé mentale publique.

Abstract This study tested the hypothesis that head and neck radiotherapy (HNRT) impacts the immunoexpression of type I collagen, bone sialoprotein (BSP) and bone morphogenetic protein 4 (BMP4), thereby leading to micromorphological changes in the dentin-pulp complex (DPC), and promoting the onset and progression of radiation caries (RC). Twenty-two demineralized sections of carious teeth (a group of 11 irradiated teeth and a control group of 11 non-irradiated teeth) extracted from 19 head and neck cancer patients were analyzed by conventional optical microscopy and immunohistochemistry to investigate the micromorphology (cellular layer hierarchy, blood vessels, odontoblasts, fibroblasts, extracellular matrix, calcification, necrosis, reactionary dentin formation, and chronic inflammation), and the patterns of staining/immunolocalization of type I collagen, BSP and BMP4 in the dental pulp of irradiated and control samples. No significant differences attributable to the direct impact of radiotherapy were detected in DPC micromorphology between the groups. In addition, the patterns of immunohistochemical staining and immunolocalization of the proteins studied did not differ between the irradiated and the control samples for type I collagen, BSP or BMP4. This study rejected the hypothesis that HNRT directly damages dentition by changing the organic components and the microstructure of the DPC, ultimately leading to RC.

Resumo Objetivo: Analisar a rede de correlações entre as dimensões do Modelo Desequilíbrio Esforço-Recompensa (DER), resiliência e qualidade de vida em policiais militares. Método: Estudo transversal realizado com 258 policiais do Batalhão de Operações Especiais (BOE) da Polícia Militar de uma cidade do Rio Grande do Sul. As escalas do Modelo DER, de resiliência e World Health Quality of Life (WHOQOL-bref) avaliaram o estresse psicossocial, resiliência e qualidade de vida. Foram realizadas estatísticas descritiva e analítica, com análise de rede. Resultados: As dimensões do Modelo DER e a resiliência se correlacionaram com todos os domínios do WHOQOL (p<0,001). O modelo de rede indicou associação negativa entre o esforço, o excesso de comprometimento e os domínios físico e meio ambiente. O domínio meio ambiente foi associado positivamente à recompensa, e a resiliência associou-se positivamente com os domínios geral, físico e psicológico. Conclusão: Concluiu-se que o estresse psicossocial interfere na qualidade de vida dos policiais militares e a resiliência pode atuar como um fator de proteção.

Abstract Objective: To analyze the network of correlations between the realms of the Effor t-Reward Imbalance (ERI) model, resilience, and quality of life among military police officers. Method: This is a cross-sectional study conducted with 258 police officers from the Special Opera tions Battalion of the Military Police in a city in Rio Grande do Sul, Brazil. The scales of the ERI model, resilience, and World Health Quality of Life (WHOQOL-bref) assessed psychosocial stress, resilience, and quality of life. Descriptive and analytical statistics were performed em ploying the network analysis. Results: The realms of the ERI model and resilience correlated with all the WHOQOL’s realms (p<0.001). The network model showed a negative association between effort, overcommitment, and physical and environmental realms. The environmental realm was positively associated with reward, while resilience was positively associated with the general, physical, and psychological realms. Conclusion: We concluded that psychosocial stress interferes in the quality of life of military police officers, and resi lience may work as a protective factor.

Resumo Objetivo: Analisar a rede de correlações entre as dimensões do Modelo Desequilíbrio Esforço-Recompensa (DER), resiliência e qualidade de vida em policiais militares. Método: Estudo transversal realizado com 258 policiais do Batalhão de Operações Especiais (BOE) da Polícia Militar de uma cidade do Rio Grande do Sul. As escalas do Modelo DER, de resiliência e World Health Quality of Life (WHOQOL-bref) avaliaram o estresse psicossocial, resiliência e qualidade de vida. Foram realizadas estatísticas descritiva e analítica, com análise de rede. Resultados: As dimensões do Modelo DER e a resiliência se correlacionaram com todos os domínios do WHOQOL (p<0,001). O modelo de rede indicou associação negativa entre o esforço, o excesso de comprometimento e os domínios físico e meio ambiente. O domínio meio ambiente foi associado positivamente à recompensa, e a resiliência associou-se positivamente com os domínios geral, físico e psicológico. Conclusão: Concluiu-se que o estresse psicossocial interfere na qualidade de vida dos policiais militares e a resiliência pode atuar como um fator de proteção.

Abstract Objective: To analyze the network of correlations between the realms of the Effor t-Reward Imbalance (ERI) model, resilience, and quality of life among military police officers. Method: This is a cross-sectional study conducted with 258 police officers from the Special Opera tions Battalion of the Military Police in a city in Rio Grande do Sul, Brazil. The scales of the ERI model, resilience, and World Health Quality of Life (WHOQOL-bref) assessed psychosocial stress, resilience, and quality of life. Descriptive and analytical statistics were performed em ploying the network analysis. Results: The realms of the ERI model and resilience correlated with all the WHOQOL’s realms (p<0.001). The network model showed a negative association between effort, overcommitment, and physical and environmental realms. The environmental realm was positively associated with reward, while resilience was positively associated with the general, physical, and psychological realms. Conclusion: We concluded that psychosocial stress interferes in the quality of life of military police officers, and resi lience may work as a protective factor.

Abstract The objective of this study was to evaluate whether the addition of a blend based on α-monolaurin mono-, di- and triglycerides of butyric acid, and lysolecithin on the performance even on diets containing reduced inclusion of oil in the diet and without the use of growth-promoting antibiotics of broilers considering the effect on health, performance, and meat. Three treatments were defined: positive control (TP: with enramycin), negative control (TN: no enramycin), and blend (T-FRA: with monolaurin and glycerides of acid butyric minus 0.8% soybean oil). At 21 days, broilers treated with TP and T-FRA obtained the lower feed conversion ratio (FC); at 35 days, T-FRA broilers obtained lower FC than TN broilers. Cholesterol levels were higher in the blood of T-FRA broilers. On day 42, levels of ROS and TBARS were lower in the intestine, muscles, and liver of T-FRA broilers. Moreover, glutathione S-transferase and total non-enzymatic antioxidants were greater at the intestinal and muscular levels. The T-FRA broilers had a lower percentage of lipids in the meat. The MIC indicated that 111mg of the blend/mL inhibited the growth of E. coli; however, the counts of total coliforms and E. coli in the feces and the broilers’ litter did not differ between treatments. In conclusion, the addition of the blend T-FRA in broiler diets was able to improve the feed conversion and maintain the other performance parameters even considering a reduction of 0.8% in the inclusion of oil.

The use of paper devices in chemical analysis has been increased due to their low cost, high surface area, portability, and biodegradability. A novel use of paper devices is proposed using thermal infrared enthalpimetry (TIE) for a rapid, green, and high throughput determination of wine acidities. The determination of total, fixed, and volatile acidities in red, white, and sparkling wine was carried out, and the results were compared with AOAC titration method. A multichannel pipette was used for reagent addition and an infrared camera for the temperature monitoring. The pipetting and temperature measurement conditions presented direct influence in the deviations among the measurements. The results obtained by TIE presented an agreement between 96 and 104% with the reference method (AOAC 962.12), for all samples. A high sample throughput was achieved reaching 480 samples h-1 for total acidity. A significant reduction in sample volume and reagent consumption was also obtained, with energy consumption up to 60 times lower in relation to the AOAC method. The association of TIE with a paper device provided a rapid, reliable and green method for the determination of acidities of wines.

Abstract In this work, a simple and fast method is proposed for the determination of ethanol in homeopathic medicines using thermal infrared enthalpimetry (TIE). Samples containing alcohol in a wide concentration range (from 5% to 95% v/v) were used. Purified water or absolute ethanol was added directly into homeopathic medicine and increase of temperature was monitored using an infrared camera. Total volume, stirring speed and dispensing rate of solutions were the most significant parameters studied for method optimization. A response surface methodology (RSM) was used for optimization of the experimental conditions. The method was validated in the following parameters: selectivity, linearity, linear range, precision (repeatability and intermediate precision), limit of detection and quantification, accuracy and robustness. Linear range was obtained from 4% to 55% (ethanol, v/v). The proposed method showed accuracy in agreement with the conventional one. The proposed method it was demonstrated a good alternative for determination of ethanol in homeopathic medicines, presenting low cost, fast analysis and agreement with the principles of green analytical chemistry.

Abstract Inflammatory dermatoses are prevalent worldwide, with impacts on the quality of life of patients and their families. The aim of this study was to determine the anti-inflammatory effects of Achyrocline satureioides oily extracts and nanocapsules on the skin using a mouse model of irritant contact dermatitis induced by croton oil, and a skin inflammation model induced by ultraviolet B (UVB) radiation. The mice were treated with 15 mg/ear oily extract (HG-OLAS) or nanocapsules (HG-NCAS) of A. satureioides incorporated into Carbopol® 940 hydrogels. We found that HG-OLAS and HG-NCAS formulations reduced ear edema in croton oil-induced lesions with maximum inhibitions of 54±7% and 74±3%, respectively. HG-OLAS and HG-NCAS formulations decreased ear edema induced by UVB radiation (0.5 J/cm2), with maximum inhibitions of 68±6% and 76±2% compared to the UVB radiation group, respectively. HG-OLAS and HG-NCAS modulated myeloperoxidase (MPO) activity after croton oil induction. Furthermore, croton oil and UVB radiation for 6 and 24 h, respectively, stimulated polymorphonuclear cells infiltration. The topical treatments reduced inflammatory processes, as shown by histological analysis. Together, the data suggest that topical application of A. satureioides oily extracts and nanocapsules produced antiedematogenic and anti-inflammatory effects. They constitute a compelling alternative for treatment of skin injuries.

RESUMO: O objetivo desse estudo foi investigar a influência da adição dos prebióticos farelo de arroz, inulina e hi-maize, na sobrevivência de Lactobacillus acidophilus em micropartículas de alginato obtidas por gelificação externa seguida de liofilização. Analisou-se o tamanho das micropartículas, a viabilidade, em simulação gastrointestinal e estabilidade, durante armazenamento. O tamanho das micropartículas variou de 127.5µm a 234.6µm. As micropartículas adicionadas dos diferentes prebióticos demonstraram um aumento na proteção do microrganismo, que apresentou maior viabilidade frente à simulação gastrointestinal. Quanto ao armazenamento em diferentes condições, a 25ºC o tratamento farelo de arroz manteve os probióticos viáveis por 30 dias. Nas condições de armazenamento -18ºC e 7°C os tratamentos contendo os prebióticoshi-maize e farelo de arroz mantiveram os microrganismos probióticos viáveis por um período de 60 dias.

ABSTRACT: The aim of this study was to investigate the influence of the addition of prebiotics rice bran, inulin and hi-maize, on the survival of Lactobacillus acidophilus in alginate microparticles obtained by external ionic gelation followed by freeze-drying. The microparticles size ranged from 127.5μm to 234.6μm. Microparticles added from the different prebiotics demonstrated an increase in the protection of the microorganism, which presented greater viability against the gastrointestinal simulation. As for storage under different conditions, rice bran treatment at 25ºC kept probiotics viable for 30 days. Under storage conditions -18°C and 7°C, treatments containing prebiotics hi-maize and rice bran maintained viable probiotic microorganisms for a period of 60 days.

Abstract Abstract: This study evaluated the effect of oregano essential oil added to the feed of commercial laying hens. This research was focused on the analysis of biochemical changes linked to hepatic function, and protein and lipid metabolism. It was used 240 laying hens (59 weeks-old) distributed in a completely randomized design of six treatments (five repetitions with eight birds each). The experiments were constituted by a control treatment (CT) with the inclusion of zinc bacitracin and five treatments of oregano essential oil (OEO: 0, 50, 100, 150, and 200 mg.kg-1), respectively. After 28 days of feeding, an increase on serum levels of total proteins and globulins was observed on groups T150 and T200, as well as an increase on albumin levels on group CT. After 84 days of feeding, a significant reduction on total proteins and albumin was observed on group T200, as well as an increase in serum triglycerides. OEO at 200 mg.kg-1 increased globulin levels on day 28, which may be considered an effect in the inflammatory response, which increases serum immunoglobulins and proteins.

Abstract One hundred fifty fungal strains were isolated from the Pampa biome for production of polyunsaturated fatty acids (PUFA). Microbial oil was produced by submerged cultivation at 120 rpm, 28 ºC for 7 days. Seven strains showed the best results for lipids accumulation, and they were selected in order to evaluate the influence of the temperature (15 and 28 ºC) on the lipid production and profile. The fungus Nigrospora sp. produced the highest lipid level: at 28 ºC were produced 11.28% of lipids and 51.94% of PUFA and at 15 ºC were produced 6.02% of lipids and 42.39% of PUFA. Plackett Burman design was performed to investigate the main effects of the temperature (15-25 ºC), pH (4.0-6.0), agitation (120-200 rpm), corn steep liquor (5-15% (v/v)) and sucrose concentrations (60-180 g L-1). The highest PUFA production (55.75%) was observed at the lowest temperatures (15 ºC), whereas the maximum lipid production was 5.54% at 25 ºC.

RESUMO: Micropartículas de Lactobacillus acidophillus La-5 (ML) e Bifidobacterium Bb-12 (MB) foram produzidas em diferentes temperaturas de secagem no spray dryer. A influência das diferentes temperaturas sobre a viabilidade, eficiência de encapsulação, atividade de água e umidade foram avaliadas. As micropartículas que apresentaram maior viabilidade foram submetidas a análises de resistência térmica, simulação gastrointestinal, estabilidade ao armazenamento, morfologia e tamanho de partícula. A temperatura de secagem de 130°C mostrou maior eficiência de encapsulação, 84.61 e 79.73% para micropartículas de Lactobacillus acidophillus (ML) e Bifidobacterium Bb-12 (MB), respectivamente. Na avaliação da resistência térmica e simulação gastrointestinal as micropartículas de Lactobacillus acidophillus La-5 (ML) apresentaram maior sobrevivência que Bifidobacterium Bb-12 (MB) nestas condições. Na viabilidade ao armazenamento somente as micropartículas Lactobacillus acidophillus La-5 (ML) mantiveram-se viáveis em todas as temperaturas avaliadas durante os 120 dias. Os tamanhos de partícula encontrados foram de 4.85 para Lactobacillus acidophillus La-5 (ML) e 8.75 para Bifidobacterium Bb-12 (MB), estando de acordo aos valores desejáveis para produtos obtidos por spray dryer. Por fim, as micropartículas de Lactobacillus acidophilus La-5 (ML) demostraram ser mais resistentes frente as condições avaliadas neste estudo.

ABSTRACT: Lactobacillus acidophillus La-5 (ML) and Bifidobacterium Bb-12 (MB) microparticles were produced at different temperatures by spray dryer. The influence of different temperatures on the viability, encapsulation efficiency, water activity and moisture were evaluated. Microparticles that presented more viability were submitted to thermal resistance, gastrointestinal simulation, storage stability, morphology and particle size analyses. Drying temperature of 130°C showed higher encapsulation efficiency, 84.61 and 79.73% for Lactobacillus acidophillus (ML) and Bifidobacterium Bb-12 (MB) microparticles, respectively. In the evaluation of thermal resistance and gastrointestinal simulation, the microparticles of Lactobacillus acidophillus La-5 (ML) presented higher survival than Bifidobacterium Bb-12 (MB) under these conditions. In storage viability only the Lactobacillus acidophillus La-5 (ML) microparticles remained viable at all evaluated temperatures during the 120 days. The particle sizes reported were 4.85 for Lactobacillus acidophillus La-5 (ML) and 8.75 for Bifidobacterium Bb-12 (MB), being in agreement with the desired values for products obtained by spray dryer. Finally, the Lactobacillus acidophilus La-5 (ML) microparticles were shown to be more resistant under the conditions evaluated in this study.

RESUMO: Diferentes frutos tropicais vêm sendo utilizados para o desenvolvimento de bebidas fermentadas, porém na bibliografia consultada há poucas referências sobre a produção de espumantes naturais de outras frutas diferentes da uva. Nesta perspectiva, o objetivo deste trabalho foi o desenvolvimento e a caracterização físico-química e volátil de um espumante natural de goiaba produzido pelo método champenoise. A determinação dos compostos voláteis foi realizada por cromatografia em fase gasosa acoplada a espectrometria de massas utilizando a técnica de microextração em fase sólida no headspace (HS-SPME) das amostras. Foram detectados 89 compostos voláteis, dos quais 51 foram identificados. Os ésteres foram os compostos voláteis predominantes em número, totalizando 26 compostos, seguido pelos álcoois (10), terpenos (9), cetonas (3) e ácidos (3). Compostos voláteis com possível atividade odorífera foram encontrados na bebida, dentre eles os ésteres octanoato de etila, 5-hexenoato de etila, acetato de fenetila, (E)-β-damascenona, (E)-cinamato de etila, acetato de 2-metil butila, 3-metil butanol, 3-(E)-hexenoato de etila e o 5-hexenoato de metila. O espumante natural produzido apresentou uma composição complexa de aroma frutado e floral. Além disso, a utilização do método tradicionalmente aplicado a uvas, o champenoise, proporcionou a fabricação de um espumante natural de goiaba com características físico-químicas equivalentes aos espumantes elaborados a partir de vinho.

ABSTRACT: Although different tropical fruit species have been used in the development of fermented beverages, there are only few references in the literature to the production of natural sparkling wines from fruits other than grapes. In this sense, the objective of the present research was the development and physicochemical and volatile characterization of a natural sparkling guava wine produced by the champenoise method. Volatile compounds were identified by gas chromatography coupled to mass spectrometry using the headspace solid-phase microextraction (HS-SPME) technique on samples. Eighty-nine volatile compounds were detected, of which 51 were identified. Esters were the predominant class of volatile compounds (a total of 26), followed by alcohols (10), terpenes (9), ketones (3), and acids (3). Volatile compounds with possible odoriferous activity were reported in the beverage, including ethyl octanoate, ethyl 5-hexenoate, phenethyl acetate, (E)-β-damascenone, (E)-ethyl cinnamate, 2-methyl butyl acetate, 3-methylbutanol, ethyl 3-(E)-hexenoate, and methyl 5-hexenoate. Natural sparkling guava wine produced showed a complex composition of fruity and floral aromas. Furthermore, the use of the champenoise method, traditionally applied to grapes, enabled the manufacture of a natural sparkling guava wine with physicochemical characteristics equivalent to those of sparkling wines made from grapes.