Objective: To describe morbidity and mortality associated with colorectal prostheses (CP) and identify factors associated with their complications during the 2007 to 2022 at INDISA Clinic. Material and Method: Retrospective cross-sectional study based on clinical registry. Results: 49 CPs were installed with 100% clearing success. 87.8% in left colon. The main etiology was malignant 91.8% and 85.7% were primary colorectal. 34 were installed with palliative intention, 12 as a bridge to elective surgery (BTS) and 3 for benign pathology. There were 13 (26.5%) patients with adverse effects (7 strictures, 4 perforations and 2 migrations). Associated mortality was 4.1% (2 patients). The complicated group was younger, with more advanced oncological stages and rectosigmoid stenosis (p < 0.005). Those under 50 years of age had 5 times higher risk of some complication (OR = 5.0; 95% CI: 1.09-23.0; p = 0.039). Discussion: The use of CPs has low short-term morbidity and mortality. Its main use is as palliative treatment or BTS in malignant colorectal obstruction. It's a high clearance rate. It is associated with less morbidity than emergency surgery, a lower ostomy rate, and a higher lymph node harvest. Installation as a bridge is also associated with a higher rate of primary anastomosis. Our group showed a higher complication rate in those with advanced and younger tumors. Conclusion: The use of CPs is safe, with low morbidity and mortality. The main indication is in obstructive colorectal neoplasia as palliative treatment. Young patients and advanced colorectal tumors with peritumoral nodes have a higher risk of complications.

Resumen Objetivo: Describir morbimortalidad asociada al uso de prótesis colorrectales (PC) e identificar factores asociados a sus complicaciones durante los años 2007 a 2022 en Clínica INDISA. Material y Método: Estudio transversal retrospectivo basado en registro clínico. Resultados: Se instalaron 49 PC con un 100% de éxito de desobstrucción. El 87,8% en colon izquierdo. La etiología principal fue la patología maligna 91,8% y el 85,7% de ellos fueron primarios colorrectales. Se instalaron 34 como tratamiento paliativo (TP), 12 como puente a cirugía electiva (PCE) y 3 por patología benigna. La tasa de complicaciones fue de 26,5% (7 estenosis, 4 perforaciones y 2 migraciones). La mortalidad asociada fue de 4,1% (2 pacientes). El grupo de complicados fue más joven, con etapas oncológicas más avanzadas y con estenosis en rectosigmoides(p < 0,005). Los menores de 50 años tuvieron 5 veces más riesgo de alguna complicación (OR = 5,0;IC95%:1,09-23,0; p = 0,039). Discusión: El uso de PC tiene baja morbimortalidad. Su uso principal es como TP o PCE en obstrucción colorrectal maligna, con alta tasa de desobstrucción. Está asociado con menor morbilidad que la cirugía de urgencia, menor tasa de ostomía y mayor cosecha ganglionar. La instalación como puente se asocia además a mayor tasa de anastomosis primaria. Nuestra serie mostró mayor tasa de complicación en aquellos con tumores avanzados y más jóvenes. Conclusión: El uso de PC es seguro y con baja morbimortalidad. La principal indicación es en neoplasia colorrectal obstructiva como TP. Tienen mayor riesgo de complicación los pacientes jóvenes y los tumores colorrectales avanzados con ganglios peri tumorales.

RESUMEN Actualmente, las investigaciones hacen énfasis en el apoyo psicológico a las familias y proveedores de salud en la unidad de cuidados críticos, más que la atención al paciente en cada una de las fases asociadas a la enfermedad. En este artículo, utilizamos un caso clínico de un paciente con COVID-19 que se encontraba en estado crítico con múltiples complicaciones para discutir y proponer protocolos de atención psicológica integral a pacientes, sus familias y proveedores de salud en unidades de cuidados intensivos. Se evidenció que, para lograr la recuperación y el alta médica del paciente, es de mucha utilizad la atención psicológica para él, los proveedores de salud y sus familiares. Muchas de las sensaciones y emociones vividas en la UCI pueden producir reacciones físicas que dificulten la estabilidad hemodinámica y el proceso de recuperación del paciente. Se han encontrado pocos estudios sobre la utilidad de la atención psicológica o psiquiátrica a pacientes con COVID-19 grave o crítico. La mayoría de los estudios informados recientemente se centran en la atención psicológica para familias y proveedores de atención médica.

ABSTRACT Currently, research emphasizes psychological support for families and health care providers in the critical care unit, rather than patient care in each of the phases associated with the disease. In this article, we use the clinical case of a patient with COVID-19, critically ill, with multiple complications that lead to the discussion and proposal of protocols for comprehensive psychological care to patients, their families, and health care providers in critical care units. It is crucial, in the efforts required to achieve patient's recovery and medical discharge, to integrate the patient into psychological care programs. Many of the sensations and emotions experienced in the ICU could produce physical reactions that hinder the patient's hemodynamic stability and recovery process. Few studies have been found on the usefulness of psychological or psychiatric care for patients with severe or critical COVID-19. Most of the recently reported studies are focused on psychological care for families and health care providers.

Abstract Antimicrobial resistance is a threat to the achievements of modern medicine and one of the most effective measures to counteract it is antimicrobial use optimization programs (AMS), in which the microbiology laboratory is one of the main components. The effective application of information technology in the processes is fundamental, but there is little information in Latin America on the development and articulation of technological tools to support AMSs. This consensus makes recommendations on the management of microbiological data for decision making. In Part I, recommendations on the use of a computerized microbiological data management system in clinical practice, data and reporting requirements in the microbiology laboratory, as well as the contents of the advanced quality management system in the laboratory are presented. In Part II, the information requirements for AMS management in intermediate, initial, and advanced stages by the laboratory and pharmacy are discussed; as well as the integration of the AMS team with the Infection Prevention and Control Committee and the information for AMS management at the management level.

Resumen La resistencia antimicrobiana es una amenaza para los logros de la medicina moderna y una de las medidas más efectivas para contrarrestarla son los programas de optimización del uso de antimicrobianos (PROA), en el cual el laboratorio de microbiología es uno de los principales componentes. La aplicación efectiva de tecnología de la información en los procesos es fundamental, pero existe poca información en Latinoamérica sobre el desarrollo y la articulación de las herramientas tecnológicas para apoyar los PROA. Este consenso hace recomendaciones sobre la gestión de los datos microbiológicos para la toma de decisiones. En la Parte I, se presentan las recomendaciones en cuanto al uso de un sistema informatizado de gestión de datos microbiológicos en la práctica clínica, los requerimientos de datos y de reporte en el laboratorio de microbiología, y los contenidos del sistema de gestión de calidad avanzado en el laboratorio. En la Parte II, se discuten los requerimientos de información para la gestión de PROA en estadios intermedios, iniciales y avanzados por el laboratorio y la farmacia; así como la integración del equipo de PROA con el Comité de Prevención y Control de Infecciones y la información para la gestión de PROA a nivel gerencial.

Abstract Antimicrobial resistance is a threat to the achievements of modern medicine and one of the most effective measures to counteract it is antimicrobial use optimization programs (AMS), in which the microbiology laboratory is one of the main components. The effective application of information technology in the processes is fundamental, but there is little information in Latin America on the development and articulation of technological tools to support AMSs. This consensus makes recommendations on the management of microbiological data for decision making. In the first part, recommendations on the use of a computerized microbiological data management system in clinical practice, data, and reporting requirements in the microbiology laboratory, as well as the contents of the advanced quality management system in the laboratory are presented. In the second part, the information requirements for AMS management in intermediate, initial, and advanced stages by the laboratory and pharmacy are discussed; as well as the integration of the AMS team with the Infection Prevention and Control Committee and the information for AMS management at the management level.

Resumen La resistencia antimicrobiana es una amenaza para los logros de la medicina moderna y una de las medidas más efectivas para contrarrestarla son los programas de optimización del uso de antimicrobianos (PROA), en los cuales el laboratorio de microbiología es uno de los principales componentes. La aplicación efectiva de tecnología de la información en los procesos es fundamental, pero existe poca información en Latinoamérica sobre el desarrollo y la articulación de las herramientas tecnológicas para apoyar los PROA. Este consenso, hace recomendaciones sobre la gestión de los datos microbiológicos para la toma de decisiones. En la Parte I, se presentan las recomendaciones en cuanto al uso de un sistema informatizado de gestión de datos microbiológicos en la práctica clínica, los requerimientos de datos y de reporte en el laboratorio de microbiología, así como y los contenidos del sistema de gestión de calidad avanzado en el laboratorio. En la Parte II, se discuten los requerimientos de información para la gestión de PROA en estadios intermedios, iniciales y avanzados por el laboratorio y la farmacia; así como la integración del equipo de PROA con el Comité de Prevención y Control de Infecciones y la información para la gestión de PROA a nivel gerencial.

Objective: To describe the experience in laparoscopic resective colorectal surgery in older than 75 years old, during 2004 to 2019 in INDISA Clinic. Material and Methods: It’s a transversal study about all the elective laparoscopic surgeries with colorectal anastomosis in elderly people, based on electronic clinical records. Results: 48 full laparoscopic surgeries, the majority by colorectal cancer (89.6%) shows optimal quality indicators about nodal harvest in 73.2%. The median duration of hospital stay after surgery was 6 days with 30 days mortality of 2.1%. These results are comparable to the international reports. Discussion: The laparoscopic approach in elderly patients has benefits over the open approach with acceptable morbility and mortality. The intraoperatory balance between surgical risk/security and oncologic prognostic it’s a factor to consider in the decision-making process besides the morbility by the age and the own functional reserve. Conclusion. It’s the first series in Chile about over 75 years patients with laparoscopic resective colorectal surgery. Is feasible and secure to do minimal invasive surgery with acceptable morbility and mortality. Only the age isn’t a contraindication to laparoscopic colorectal surgery. It’s necessary more studies with mayor number of patients to known better the national results and long-term results.

Resumen Objetivo: Describir la experiencia en cirugía colorrectal resectiva laparoscópica en mayores de 75 años durante los años 2004 a 2019 en Clínica INDISA. Material y Método: Estudio transversal de las cirugías electivas por vía laparoscópica en mayores de 75 años, en los que se realizó anastomosis colorrectal, basado en el registro clínico electrónico. Resultados: Un total de 48 cirugías completamente laparoscópicas, la mayoría por cáncer colorrectal (CCR) (89,6%), mostraron indicadores de calidad óptima en la cosecha ganglionar en el 73,2%. Con una mediana de estadía de 6 días y una mortalidad a 30 días de 2,1% (un caso), comparable a las series internacionales. Discusión: El abordaje laparoscópico en pacientes ancianos tiene beneficios por sobre la vía abierta y presenta morbimortalidad aceptable. El balance intraoperatorio entre riesgo/seguridad quirúrgica y pronóstico oncológico es un factor a tener en cuenta en la toma de decisiones, junto con las patologías inherentes al grupo etario y la reserva funcional de cada paciente en particular. Conclusión: Se trata de la primera serie nacional de pacientes sobre 75 años sometidos a cirugía colorrectal resectiva electiva vía laparoscópica. Es factible y seguro en estos pacientes realizar cirugía mínimamente invasiva con morbimortalidad aceptable. La edad por sí sola no representa una contraindicación para la cirugía colorrectal laparoscópica. Es necesario contar con estudios de mayor volumen para conocer mejor la realidad nacional y los resultados a largo plazo.

Abstract The concept of Natural Capital of the Romerillo forests (NCR) has been little studied in relation to the Education of this important type of natural capital. However, currently, many researchers have argued that Education can contribute significantly to the teaching and learning of this natural capital. By investigating how the concept has been studied, this article has attempted to establish the direction, understanding and use of Education that the NCR may take in the future, within the context of research into an Intangible Creative Ecological Economy (ICEE). This study used an exploratory research methodology with secondary information, in combination with chronological, conceptual and thematic literature review techniques. The literature review indicates that Education on NCR has been used in three areas of research to explain or address a particular construct: (1) promotion of formal and informal programs for use of the intangible environmental services, (2) development of an ICEE that promotes biotrade of intangible services from the Romerillos forests, and (3) short- and long-term conservation of the Romerillos forests. For future studies, it is recommended to address the concept of Education in “Intangible Forest Natural Capital” as a strategy for the sustainable use of the intangible environmental services of the NCR, aiming to mitigate deforestation and preserve the forests of the Romerillos Andean.

Abstract Objective To present a rare case of anal squamous cell carcinoma (SCC) in a young pregnant woman. Material and Methods The information was collected based on the clinical record after obtaining the informed consent of the patient. Results This is the case of a 34-year-old pregnant woman with stage IIIB anal SCC. She was treated with chemotherapy before delivery, followed by cesarean section and chemoradiotherapy after birth. The patient experienced complete clinical and imaging regression after treatment, but she presented early tumor regrowth requiring abdominoperineal amputation, with negative surgical margins. Discussion The treatment of anal cancer in pregnant women is based on a multidisciplinary approach, adapting current clinical guidelines. It establishes a challenge from an ethical point of view that must consider the impact on the health of the mother and the fetus. Anal screening in carriers of cervical pathology due to papilloma virus would be a good tool for early detection. SCC (SCC 34yearold yearold 34 year old delivery birth amputation margins approach guidelines fetus detection 3

Abstract In this work, we studied 46 bacterial isolates to determine their potential for polyethylene terephthalate (PET) degradation. Only eight lipase-positive isolates were pre-selected to continue analysis. The isolates belonged to the genera Micrococcus, Acinetobacter, Pseudomonas, Bacillus, and Pseudochrobactrum. In addition to lipase, other enzymatic activities such as protease, carboxymethyl cellulase, xylanase, and chitinase were detected. PET sheets were pretreated with temperature, UV light, HNO3, and Stenotrophomonas pavanii. All isolates were tested against pretreated PET for 60 days. All isolates displayed lipase activity, and some hydrolase activities. The chemical changes of PET sheets were determined using Fourier-transform infrared spectroscopy. Peaks observed at 3000-3500 cm-1 corresponding to hydroxyl group suggested degradation. The physical changes of PET observed with the naked eye and with SEM consisted of erosion, bubbles, scales, bumps, pits, and a shiny surface. Pseudomonas soli was selected as the best PET degrader, according to the notable changes in the infrared spectrum and macroscopic changes compared to the other genera and the control. In addition, this isolate did not show xylanase activity and it lacks the chitinase gene too. It is possible that not only lipase activity could be associated with the PET degradation, but also another enzymatic activity not detected in this study. Although the genus Pseudomonas has been reported as a plastic degrader, the P. soli species is novel in the degradation of PET, as well as the other genera mentioned here. Finally, these findings contribute to the knowledge of bacterial species for PET degradation. work 4 (PET lipasepositive positive preselected pre analysis Micrococcus Acinetobacter Bacillus Pseudochrobactrum protease cellulase temperature light HNO3 HNO pavanii 6 days Fouriertransform Fourier transform spectroscopy 30003500 3000 3500 3000-350 cm1 cm 1 cm- erosion bubbles scales bumps pits surface degrader control too study P here Finally 3000350 300 350 3000-35 300035 30 35 3000-3 30003 3 3000-

Resumen: Introducción: el tratamiento quirúrgico de la escoliosis idiopática del adolescente (EIA) implica varias maniobras ya descriptas para la alineación de la curva escoliótica y posteriormente la derotación vertebral. Objetivo: El objetivo es conseguir la mayor corrección posible, preservando así los parámetros del equilibrio sagital y dejando el mayor número de segmentos móviles. El propósito del estudio es verificar la técnica de derotación combinada implementada en la Clínica Reespalda y su tasa de corrección en la escoliosis idiopática, y evaluar la densidad de los implantes. Método: estudio observacional retrospectivo recolectado entre 2021 y 2023, con seguimiento de 6 meses, Study conducted by the Re-Espalda que incluye datos clínicos y radiológicos. Se utilizó estadística descriptiva e inferencial (R 4.3.2). Técnica de derotación: instrumentación transpedicular posterior con monitorización neurofisiológica intraoperatoria, insertando una varilla en un contorno sagital adecuado en el lado convexo. Herramienta de reducción especializada con anclajes sin fijación, realizando una traslación vertebral. Luego se realiza un desrotación global con una sola varilla, fijando anclajes. Colocación de la varilla cóncava con hipocifosis leve. Distracción en el lado cóncavo y compresión en el lado convexo. Resultados: Fueran totalizados 25 casos (no. 15 de Lenke 1, no. 5 de Lenke 3 y no. 5 de Lenke 5). Ángulo medio de Cobb preoperatorio de 60,44º y ángulo medio de Cobb postoperatorio de 22,22º, con una tasa de corrección media de 67,45º. La alta densidad de tornillos se relacionó con una mejor tasa de corrección (p=0,0266) en Lenke 1, mientras que el 100% de los Lenke 3 y 5 fueron de alta densidad. Conclusión: La técnica de derotación combinada alcanzó una tasa de corrección exitosa, sin embargo, se necesitó alta densidad de tornillos para conseguirlo. Nivel de Evidencia IV; Serie de Casos. Resumen Introducción EIA (EIA vertebral Objetivo posible móviles implantes Método 202 2023 meses ReEspalda Re Espalda radiológicos R 4.3.2. 432 4.3.2 . 4 2 4.3.2) intraoperatoria convexo fijación leve Resultados no (no 1 5. 5) 6044º º 60 44º 2222º 22 22º 22,22º 6745º 67 45º 67,45º p=0,0266 p00266 p 0 0266 (p=0,0266 100 Conclusión exitosa embargo conseguirlo IV Casos 20 43 4.3. p=0,026 p0026 026 (p=0,026 10 4.3 p=0,02 p002 02 (p=0,02 4. p=0,0 p00 (p=0,0 p=0, p0 (p=0, p=0 (p=0 p= (p= (p

Resumo: Introdução: O tratamento cirúrgico da escoliose idiopática do adolescente (EIA) envolve diversas manobras já descritas para alinhamento da curva escoliótica e posterior derotação vertebral. Objetivo: O objetivo é conseguir a maior correção possível, preservando assim os parâmetros do equilíbrio sagital e deixando o maior número de segmentos móveis. O objetivo do estudo é verificar a técnica de derotação combinada implementada na Clínica Reespalda e sua taxa de correção na escoliose idiopática, e avaliar a densidade dos implantes. Método: Estudo observacional retrospectivo coletado entre 2021 e 2023, com acompanhamento de 6 meses, incluindo dados clínicos e radiológicos. Foram utilizadas estatísticas descritivas e inferenciais (R 4.3.2). Técnica de derotação: instrumentação transpedicular posterior com monitorização neurofisiológica intraoperatória, inserindo uma haste em contorno sagital adequado no lado convexo. Ferramenta de redução especializada com âncoras sem fixação, realizando translação vertebral. Em seguida, é realizada uma derotação global com uma única haste, fixando as âncoras. Colocação da haste côncava com hipocifose leve. Distração no lado côncavo e compressão no lado convexo. Resultados: Foram totalizados 25 casos (nº 15 do Lenke 1, nº 5 do Lenke 3 e nº 5 do Lenke 5). Ângulo de Cobb médio pré-operatório de 60,44º e ângulo de Cobb médio pós-operatório de 22,22º, com taxa média de correção de 67,45º. A alta densidade de parafusos foi relacionada a uma melhor taxa de correção (p=0,0266) no Lenke 1, enquanto 100% dos Lenke 3 e 5 foram de alta densidade. Conclusão: A técnica de depreciação combinada alcançou uma taxa de correção bem sucedida, no entanto, foi necessária uma alta densidade de parafusos para conseguir isso. Nível de Evidência IV; Série de Casos. Resumo Introdução EIA (EIA vertebral Objetivo possível móveis implantes Método 202 2023 meses radiológicos R 4.3.2. 432 4.3.2 . 4 2 4.3.2) intraoperatória convexo fixação seguida leve Resultados 1 5. 5) préoperatório pré operatório 6044º º 60 44º pósoperatório pós 2222º 22 22º 22,22º 6745º 67 45º 67,45º p=0,0266 p00266 p 0 0266 (p=0,0266 100 Conclusão sucedida entanto isso IV Casos 20 43 4.3. p=0,026 p0026 026 (p=0,026 10 4.3 p=0,02 p002 02 (p=0,02 4. p=0,0 p00 (p=0,0 p=0, p0 (p=0, p=0 (p=0 p= (p= (p

ABSTRACT Introduction: The surgical treatment of adolescent idiopathic scoliosis (AIS) involves several maneuvers already described to align the scoliotic curve and, subsequently, vertebral derotation. Objective: The goal is to be able to achieve the greatest possible correction, thus preserving sagittal balance parameters and leaving the greatest number of mobile segments possible. The aim of the study is to verify the combined derotation technique implemented at the Reespalda Clinic and its correction rate in idiopathic scoliosis and to evaluate implant density to achieve it. Method: Observational retrospective study collected between 2021 and 2023, with a 6-month follow-up, including clinic and radiologic data. Descriptive and inferential statistics were analyzed (R 4.3.2). Derotation technique: posterior transpedicular instrumentation with intraoperative neurophysiological monitoring, inserting a rod in a proper sagittal contour on the convex side. Specialized reduction tool with loosely affixing anchors, doing a vertebral translation. Then, a global derotation with only one rod, fixing anchors. Placing the concave rod with mild hypokyphosis. Distraction on the concave side, and compression on the convex side. Results: 25 cases were totalized (no. 15 of Lenke 1, no. 5 of Lenke 3 and no. 5 of Lenke 5). Preoperative Cobb mean angle of 60.44º and postoperative Cobb mean angle of 22.22º, with a mean correction rate of 67.45º. High screw density was related to a better correction rate (p=0.0266) in Lenke 1. Meanwhile, 100% of Lenke 3 and 5 were high density. Conclusion: Combined derotation technique reached a successful correction rate; however, high-density screw was needed to achieve it. Level of Evidence IV; Cases of Series. Introduction AIS (AIS subsequently Objective it Method 202 2023 6month month 6 followup, followup follow up, up follow-up data R 4.3.2. 432 4.3.2 . 4 2 4.3.2) monitoring side anchors translation Then hypokyphosis Results no (no 1 5. 5) 6044º º 60 44º 2222º 22 22º 22.22º 6745º 67 45º 67.45º p=0.0266 p00266 p 0 0266 (p=0.0266 Meanwhile 100 Conclusion however highdensity IV Series 20 43 4.3. p=0.026 p0026 026 (p=0.026 10 4.3 p=0.02 p002 02 (p=0.02 4. p=0.0 p00 (p=0.0 p=0. p0 (p=0. p=0 (p=0 p= (p= (p

Resumen Introducción: La colocación de catéteres intravasculares venosos umbilicales (CVU) y arteriales (CAU) es el procedimiento invasivo más frecuente en la unidad de cuidados intensivos neonatales (UCIN). Para introducirlos se utilizan las fórmulas de Wright y de Shukla, aunque esta última podría estar asociada con una inserción incorrecta, especialmente en neonatos < 1500 g. El objetivo de este estudio fue determinar mediante radiografía de tórax cuál fórmula es mejor para la correcta colocación de catéteres umbilicales en recién nacidos ≤ 1500 g. Métodos: Se incluyeron los pacientes ingresados en la UCIN de un hospital de segundo nivel entre 2021-2022 que recibieron CVU o CAU mediante las fórmulas de Wrigth o Shukla. Resultados: Se incluyeron en total 129 recién nacidos: 78 CVU y 51 CAU. En CVU, Wright 50% y Shukla 36.8% tuvieron localización correcta, p = 0.24. En las CAU, Wright 56.6% y Shukla 52.4% tenían una ubicación correcta, p = 0.76. En CVU con peso < 1000 g, Wright 36.4% y Shukla 33.3% bien situados, p = 0.58. En CVU > 1000 g, Wright 66.6% y Shukla 38.4% bien situados, p = 0.065. En CAU < 1000 g, Wright 45% y Shukla 42.9%, p = 0.63. En CAU con peso > 1000 g, Wright 80% y Shukla 57.1%, p = 0.23. Conclusiones: La colocación del CVU fue 13% mejor con la fórmula de Wright. La fórmula de Wright superó en el 29% la colocación del CVU en los neonatos > 1000 g en comparación con la de Shukla, aunque no hubo diferencia significativa debido al tamaño de la muestra.

Abstract Background: Intravascular venous (VUC) or arterial (AUC) umbilical catheter placement is the most frequent invasive procedure in the neonatal intensive care unit (NICU). Either Wright’s or Shukla’s formula is used to introduce the catheters. However, Shukla’s formula is associated with incorrect insertion, especially for newborns < 1500 g. This study aimed to determine by chest X-ray if Wright’s formula is better than Shukla’s formula for the correct placement of umbilical catheters in newborns ≤ 1500 g. Methods: We included patients admitted to the NICU of a secondary-level hospital between 2021-2022 who received VUC or AUC through the Wright or Shukla formulas. Results: A total of 129 newborns were included: 78 with VUC and 51 with AUC. In VUC, 50% with Wright and 36.8% with Shukla formulas had the correct location, (p = 0.24). In AUC, 56.6 % with Wright and 52.4% with Shukla formulas were placed correctly placed, (p = 0.76). VUC with weight < 1000 g were correctly placed in 36.4% with Wright and 33.3% with Shukla formulas (p = 0.58). VUC in newborns > 1000 g were correctly placed in 66.6% with Wright and 38.4% with Shukla formulas (p = 0.065). AUC in newborns < 1000 g were correctly placed in 45% using Wright and 42.9% Shukla formulas (p = 0.63). AUC in newborns > 1000 g were correctly placed in 80% using Wright and 57.1% Shukla formulas (p = 0.23). Conclusions: We found 13% more correctly placed VUC using Wright’s formula. Moreover, Wright’s formula was 29% above Shukla’s VUC placement in neonates > 1000 g, although there was no significant difference due to the sample size.

Abstract Labor is the transition from a state of inactivity and muscle relaxation to a state of muscle excitation, in which the muscular layer of the uterus (myometrium) performs increasingly coordinated contractions to deliver the fetus and expel the placenta. During the onset of labor, the myometrium undergoes a series of physiological, biochemical, and molecular changes, allowing the tissue to transition from a quiescent state to a contractile phenotype that will support labor. This is partly caused by the action of the hormones progesterone, estradiol, and oxytocin. In general, progesterone maintains the quiescence of the myometrium during pregnancy by decreasing the expression of proinflammatory molecules and contraction-associated proteins. In contrast, at the end of pregnancy, estradiol induces the expression of these molecules. For its part, oxytocin induces an increase in intracellular calcium concentration to carry out the contractions of uterine myocytes. The objective of this review is to present a summary of the molecular mechanisms involved in regulating myometrial cell activity through the hormones progesterone, estradiol and oxytocin, as well as to discuss the perspectives of this exciting area of research.

Resumen El trabajo de parto es la transición de un estado de inactividad y relajación muscular a un estado de excitación, en el cual la capa muscular del útero (miometrio) realiza crecientes contracciones coordinadas para llevar a cabo la expulsión del feto y la placenta. Durante el inicio del trabajo de parto, el miometrio experimenta una serie de cambios fisiológicos, bioquímicos y moleculares, pasando de un estado de quiescencia a un fenotipo contráctil que inducirá el parto. En parte, esto es provocado por la acción de las hormonas progesterona, estradiol y oxitocina. En general, la progesterona mantiene la quiescencia del miometrio durante el embarazo al inhibir la expresión de moléculas proinflamatorias y proteínas asociadas a la contracción, mientras que al término del embarazo, el estradiol induce la expresión de dichas moléculas. Por su parte, la oxitocina induce un aumento en la concentración de calcio intracelular para llevar a cabo las contracciones de los miocitos uterinos. El objetivo del presente trabajo es presentar un resumen acerca de los mecanismos moleculares involucrados en la regulación de la actividad de las células miometriales por medio de las hormonas progesterona, estradiol y oxitocina, así como discutir las perspectivas de esta interesante área de investigación.

Abstract The aim of this study was to determine the association between oral health-related quality of life (OHRQoL) and social marginalization in people aged 60 years and older enrolled in social security in Mexico. A cross-sectional and analytical study was carried out in older adults. To assess the OHRQoL, the OHIP-14 instrument was applied, and the degree of social marginalization and sociodemographic characteristics were analyzed. Measures of central tendency and dispersion, simple frequencies and proportions were estimated. Student’s t-test was used for comparison of means, and prevalence ratio (PR) and logistic regression were used to assess associations, all with a significance value of 0.05 and 95% confidence intervals. Perceived OHRQoL in the population measured through the OHIP-14 reached an average value of 9.84 ± 8.91, with the highest value in the dimension of physical pain (2.06 ± 1.91). Perceived treatment need was higher among people with social marginality (p = 0.011). The multivariate analysis shows that marginalized people have a lower OHRQoL. Socially marginalized older adults showed a low a better perception of OHRQoL, independent of demographic and clinical factors. healthrelated health related (OHRQoL 6 Mexico crosssectional cross sectional OHIP14 OHIP 14 OHIP-1 applied analyzed dispersion estimated Students Student s ttest t test means PR (PR associations 005 0 05 0.0 95 intervals 984 9 84 9.8 891 8 91 8.91 2.06 206 2 06 (2.0 1.91. 191 1.91 . 1 1.91) p 0.011. 0011 0.011 011 0.011) factors OHIP1 OHIP- 00 0. 98 9. 89 8.9 2.0 20 (2. 19 1.9 001 0.01 01 8. 2. (2 1. (

ABSTRACT Artemisia ludoviciana contains several medically important metabolites, but few studies have been reported on its effects on insects of agricultural importance. This article aimed to know the biological effects of a methanolic extract of A. ludoviciana on neonatal larvae of Spodoptera frugiperda, where column chromatography was obtained to fractionate the crude oil and mass gas chromatography to identify metabolites, the extract, and the 90:10 mobile phase showed low mortality of about 30% at a concentration of 1 mg/mL, but a weight loss in neonatal larvae of more than 50% was lost, confirming a significant antifeedant activity. GC-MS analysis revealed the presence of terpenoids such as limonene, thujone, camphor, borneol, and borneol acetate, which are associated with an insecticidal and antifeedant activity. Therefore, the methanolic extracts of A. ludoviciana can be a friendly alternative for insect pest control.

RESUMEN Artemisia ludoviciana contiene varios metabolitos de importancia médica, sin embargo, se han informado pocos estudios sobre sus efectos en insectos de importancia agrícola. El objetivo de este trabajo fue conocer los efectos biológicos de un extracto metanólico de A. ludoviciana sobre larvas neonatas de Spodoptera frugiperda en donde se utilizó cromatografía de columna para fraccionar el crudo y cromatografía de gases masas para identificación de metabolitos, dando como resultados que el extracto y la fase móvil 90:10 mostraron una baja mortalidad de alrededor del 30 % a una concentración de 1 mg/mL, sin embargo, se observó una pérdida de peso en las larvas neonatas de más de 50 %, corroborando una actividad antialimentaria significativa, y el análisis GC-MS reveló la presencia de terpenoides como: limoneno, tujona, alcanfor, borneol y acetato de borneol, asociados con el efecto insecticida y la actividad antialimentaria. Por lo tanto, los extractos metanólicos de A. ludoviciana, pueden ser una alternativa amigable para el control de insectos plagas.

Resumen Introducción. La capacidad física cardiorrespiratoria es un predictor de mortalidad por enfermedad cardiovascular y por todas las causas. Su diagnóstico en diferentes grupos tiene utilidad clínica y en salud pública. Objetivo. Evaluar la validez y reproducibilidad de un método sin ejercicio implementado por la NASA (National Aeronautics and Space Administration), para estimar el volumen máximo de oxígeno (VO2máx) consumido en adultos universitarios. Materiales y métodos. Estudio de validación de una prueba que incluyó 94 individuos sanos de ambos sexos (18 a 55 años). La prueba de referencia fue la ergoespirometría. La validez y la reproducibilidad se evaluaron mediante el coeficiente de correlación intraclase (Intraclass Correlation Coefficient, CCI) y el método de Bland-Altman. Resultados. Del total de los individuos incluidos en el estudio, 48,9 % fueron mujeres. La media de edad de los participantes fue de 30,54 ± 9,33 años y, la del VO2máx, fue de 41,29 ± 9,54 mlO2.kg-1.min-1. Se encontró una diferencia de medias de VO2máx entre la ergoespirometría y el estimado por el método implementado por la NASA de 3,41 ± 5,64 mlO2.kg-1.min-1. La concordancia entre los dos métodos fue buena, con un coeficiente de correlación intraclase de 0,858 (IC95% 0,672-0,926). El porcentaje de error fue del 29,70 %. La reproducibilidad de las dos estimaciones por el método implementado por la NASA fue excelente, con un coeficiente de correlación intraclase de 0,986 (IC95% 0,927-0,995). Conclusiones. El método NASA es válido y reproducible para estimar el VO2máx en adultos universitarios; además, es seguro y de fácil aplicación. Se recomienda la estimación de la capacidad física cardiorrespiratoria para mejorar la tamización en los programas de riesgo cardiometabólico e implementar intervenciones oportunas.

Abstract Introduction: Cardiorespiratory fitness is a predictor of cardiovascular and all-cause mortality. Its assessment in different groups has clinical and public health usefulness. Objective: To evaluate the validity and reproducibility of a no-exercise method [National Aeronautics and Space Administration (NASA) method] to estimate the maximum oxygen consumption (VO2máx) in college adults. Materials and methods: This study included 94 healthy individuals of both sexes (1855 years). The gold standard was ergospirometry. The validity and reproducibility were evaluated with the intraclass correlation coefficient (ICC) and the Bland-Altman method. Results: Among the participants, we found a mean age of 30.54 ± 9.33 years and a VO2máx of 41.29 ± 9.54 ml O2kg-1-min-1; 48.9 % were women. A mean difference of VO2máx between ergospirometry and that estimated by the NASA method of 3.41 ± 5.64 ml O2.kg-1. min-1 was found. The concordance between the two methods was good, with an ICC of 0.858 (CI95% 0.672-0.926). The percentage of error was 29.70 %. The reproducibility of the two estimates by the NASA method was excellent, with an ICC of 0.986 (CI95% 0.927-0.995). Conclusions: The NASA method is valid and reproducible to estimate VO2máx in college adults. In addition, it is safe and easy to apply. Estimating cardiorespiratory fitness is recommended to improve screening in cardiometabolic risk programs and to implement timely interventions.