RESUMO A doença renal crônica (DRC) representa um dos principais problemas de saúde pública da atualidade. A dosagem da creatinina sérica e a estimativa da taxa de filtração glomerular (TFG) são as principais ferramentas para avaliação da função renal. Para a estimativa da TFG, existem diversas equações, sendo a mais recomendada a CKD-EPI (Chronic Kidney Disease – Epidemiology). Existem ainda algumas controvérsias com relação à dosagem da creatinina sérica e da estimativa da TFG, uma vez que vários fatores podem interferir nesse processo. Uma importante mudança recente foi a retirada da correção por raça das equações para estimativa da TFG, que superestimavam a função renal, e consequentemente retardavam a implementação de tratamentos como diálise e transplante renal. Neste documento de consenso da Sociedade Brasileira de Nefrologia e Sociedade Brasileira de Patologia Clínica e Medicina Laboratorial são revisados os principais conceitos relacionados à avaliação da função renal, possíveis controvérsias existentes e recomendações para a estimativa da TFG na prática clínica. DRC (DRC atualidade (TFG CKDEPI CKD EPI Chronic Epidemiology. Epidemiology . Epidemiology) processo clínica

ABSTRACT Chronic kidney disease (CKD) represents one of today’s main public health problems. Serum creatinine measurement and estimation of the glomerular filtration rate (GFR) are the main tools for evaluating renal function. There are several equations to estimate GFR, and CKD-EPI equation (Chronic Kidney Disease – Epidemiology) is the most recommended one. There are still some controversies regarding serum creatinine measurement and GFR estimation, since several factors can interfere in this process. An important recent change was the removal of the correction for race from the equations for estimating GFR, which overestimated kidney function, and consequently delayed the implementation of treatments such as dialysis and kidney transplantation. In this consensus document from the Brazilian Societies of Nephrology and Clinical Pathology and Laboratory Medicine, the main concepts related to the assessment of renal function are reviewed, as well as possible existing controversies and recommendations for estimating GFR in clinical practice. CKD (CKD todays today s problems (GFR CKDEPI EPI Epidemiology process transplantation Medicine reviewed practice

A metallic surface can exhibit low wettability through the formation of a rough structure with micro/nanometric dimensions combined with a surface energy-reducing agent. When the contact and sliding angles are ≥ 150° and ≤ 10°, respectively, the surface is classified as superhydrophobic (SHS). The study aimed to electrodeposit a microstructure with micronanometric roughness of nickel, stearic acid (Al/Ni-SA), and nickel, stearic acid with multi-layered carbon nanotubes (Al/Ni-SA-MWCNT) on aeronautical aluminum 7050. The obtained contact angles were close to 160°, and sliding angles were less than 1°, characterizing the superhydrophobic and self-cleaning nature. Freezing tests were conducted, and the deposit with MWCNT showed longer freezing times at -22°C. The Al/Ni-SA-MWCNT condition also exhibited the best corrosion resistance with corrosion current superior by two orders of magnitude compared to the Al/Ni-Sa condition. The coatings demonstrated impressive performance, achieving corrosion inhibition efficiencies close to 100%. micro nanometric energyreducing energy reducing agent 150 10 10° respectively SHS. SHS . (SHS) nickel Al/NiSA, AlNiSA Al/Ni SA , Al Ni (Al/Ni-SA) multilayered multi layered Al/NiSAMWCNT AlNiSAMWCNT (Al/Ni-SA-MWCNT 7050 160 160° 1 1° selfcleaning self cleaning nature conducted 22°C. 22C C 22 -22°C Al/NiSa AlNiSa Sa performance 100 100% 15 (SHS NiSA Al/NiSA AlNi (Al/Ni-SA NiSAMWCNT 705 16 22°C 2 NiSa 70 7

ABSTRACT Biological soil crusts (BSC) are commonly found in soils in the drylands regions, which can influence stabilization, water retention, nutrient cycling (particularly carbon (C) and nitrogen (N) dynamics), and several ecological processes. However, the composition of BSC in Brazilian soils undergoing the desertification process remains poorly understood. This study aimed to characterize the bacterial community in BSC formed in a Brazilian semiarid region under the desertification process. Thus, a highly desertified region was selected from which 34 BSC samples were collected. The total DNA of the BSC was extracted from 0.5 g samples, and the bacterial community was sequenced by a Next Generation Sequencing (NGS) platform (Miseq – Illumina®) using universal primers (515F and 806R). Bioinformatic analysis was carried out in QIIME (v.1.9), and the Operational Taxonomic Units (OTU) table was constructed following the Sumaclust methodology. The pH of BSC, C, N, and phosphorus contents was analyzed. Our study identified a diverse bacterial community in the BSCs. Cyanobacteria, Chloroflexi, and Proteobacteria phyla presented the greatest relative abundance (%) across the samples. Cyanobacteria were dominated by the orders Nostocales and Leptolyngbyales. The prediction of the putative functions found that mostf OTU were related to phototrophy, photosynthetic cyanobacteria, and photoautotrophy. The study found correlations between bacterial phyla and BSC properties, with Cyanobacteria positively related to C. Chloroflexi, Armatimonadetes, and WPS-2 were negatively correlated with C and N contents. These results suggest the critical roles bacteria communities play in BSCs from the Caatinga biome and highlight the potential impact of environmental factors on their diversity and functions. (BSC regions stabilization retention particularly (C (N dynamics, dynamics , dynamics) processes However understood Thus 3 collected 05 0 5 0. NGS (NGS Miseq Illumina® Illumina 515F F 806R. 806R R . 806R) v.1.9, v19 v v.1.9 1 9 (v.1.9) (OTU methodology analyzed Chloroflexi % (% Leptolyngbyales phototrophy cyanobacteria photoautotrophy properties Armatimonadetes WPS2 WPS 2 WPS- v1 v.1. (v.1.9 ( v.1 (v.1. v. (v.1 (v. (v

Abstract This study aimed to evaluate the phenophases of Cenostigma pyramidale (Tul.) Gagnon & G.P.Lewis in an area of Caatinga in the semi-arid region of Paraíba, Brazil. Field research was carried out in the municipality of Sumé, in the Experimental Space reserved for Studies on Ecology and Dynamics of the Caatinga - Area I of the Laboratory of Ecology and Botany - LAEB/CDSA/UFCG (7°39'38.8'' S and 36°53'42.4'' W; 538 m altitude). A total of 96 plots of 10 x 10 m were arranged in the study area, seeking to identify one specimen of C. pyramidale per plot. Monitoring was performed monthly from March 2019 to February 2020, recording budding, senescence, flower bud, flowering, and fruiting data. The Fournier intensity percentage and the activity index were analyzed. The highest budding intensity index was observed when there was a gradual increase in precipitation. Senescence had the highest peaks of intensity in the months with the absence or reduced levels of precipitation. Flower bud and flowering were practically equal throughout the monitoring period. The highest fruiting intensity occurred in the month with the highest precipitation. Regarding the budding activity index, all specimens expressed this phenophase as soon as the precipitation levels increased. In the dry period, all specimens showed senescence. Flower bud and flowering phenophases occurred synchronously. Fruiting had a higher activity index in periods of higher precipitation. Thus, it was observed that precipitation has a great influence on phenological events. Tul. Tul (Tul. GPLewis G P Lewis semiarid semi arid Paraíba Brazil Sumé LAEBCDSAUFCG LAEB CDSA UFCG 7°3938.8 739388 7°39 38.8 7 39 38 8 (7°39'38.8' 36°5342.4 3653424 36°53 42.4 36 53 42 4 36°53'42.4' W altitude. altitude . altitude) 9 1 C plot 201 2020 senescence data analyzed period increased synchronously Thus events (Tul 3938 7°3938. 73938 739 7°3 388 38. 3 (7°39'38.8 5342 36°5342. 365342 3653 36°5 424 42. 5 36°53'42.4 20 202 393 7°3938 7393 73 7° (7°39'38. 534 36°5342 36534 365 36° 36°53'42. 2 7°393 (7°39'38 36°534 36°53'42 (7°39'3 36°53'4 (7°39' 36°53' (7°39 (7°3 (7° (7 (

Resumo A doença cerebrovascular extracraniana tem sido intensamente investigada em todo o mundo, sendo tema de suma importância para os cirurgiões vasculares. A presente Diretriz foi elaborada pela Sociedade Brasileira de Angiologia e Cirurgia Vascular (SBACV) em sucessão à Diretriz de 2015. As doenças de etiologia não ateroscleróticas não foram incluídas nesse documento. O objetivo desta Diretriz é congregar as evidências mais robustas nessa área para auxiliar os especialistas no processo decisório do tratamento. Foi utilizada a metodologia AGREE II e o sistema da Sociedade Europeia de Cardiologia para as recomendações e níveis de evidências. As recomendações foram graduadas de I a III, e os níveis de evidência classificados em A, B e C. A presente Diretriz foi dividida em 11 capítulos, que tratam dos vários aspectos da doença cerebrovascular extracraniana: diagnóstico, tratamentos e complicações, de forma atualizada e com as recomendações propostas pela SBACV. mundo vasculares SBACV (SBACV 2015 documento tratamento III C 1 capítulos diagnóstico complicações 201 20 2

Abstract Extracranial cerebrovascular disease has been the subject of intense research throughout the world, and is of paramount importance for vascular surgeons. This guideline, written by the Brazilian Society of Angiology and Vascular Surgery (SBACV), supersedes the 2015 guideline. Non-atherosclerotic carotid artery diseases were not included in this document. The purpose of this guideline is to bring together the most robust evidence in this area in order to help specialists in the treatment decision-making process. The AGREE II methodology and the European Society of Cardiology system were used for recommendations and levels of evidence. The recommendations were graded from I to III, and levels of evidence were classified as A, B, or C. This guideline is divided into 11 chapters dealing with the various aspects of extracranial cerebrovascular disease: diagnosis, treatments and complications, based on up-to-date knowledge and the recommendations proposed by SBACV. world surgeons SBACV, SBACV , (SBACV) 201 Nonatherosclerotic Non atherosclerotic document decisionmaking decision making process III A B C 1 diagnosis complications uptodate up date (SBACV 20 2

Resumo Objetivo Mapear a produção científica sobre as estratégias educativas e os conteúdos abordados na educação de pessoas vivendo com HIV. Métodos Esta é uma revisão de escopo em que a seleção dos artigos foi realizada em abril de 2021 e atualizada em outubro de 2022 em dez fontes de dados; a revisão seguiu os pressupostos estabelecidos pelo Joanna Briggs Institute e o checklist dos Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews. Os resultados foram analisados descritivamente e sintetizados em um quadro. Resultados Foram selecionados 17 estudos com publicação predominante em 2017; Estados Unidos da América e Brasil foram os países com a maior quantidade de produções. A maioria dos estudos buscou avaliar o impacto e a eficácia das estratégias e desenvolver ou validar instrumentos de educação em saúde e atividades de prevenção. Em relação ao conteúdo abordado pelas estratégias, foram formadas cinco categorias: orientação inicial sobre HIV/AIDS, cuidados gerais, vida saudável, saúde sexual e suporte emocional. As estratégias educativas que se destacaram em relação à maior adesão dos pacientes ao tratamento estão relacionadas com o desenvolvimento de sistemas, programas e multimídia. As cartilhas promoveram empoderamento e autonomia de pessoas vivendo com HIV. Conclusão Foram mapeadas as principais estratégias educativas, com destaque para cartilhas, material impresso, recursos multimídia, sistemas, formulários e oficinas/workshops, abordando orientação inicial sobre HIV/AIDS, tratamento farmacológico, cuidados gerais, vida saudável, saúde sexual e suportes social e emocional. HIV 202 dados MetaAnalyses Meta Analyses Reviews quadro 1 2017 produções prevenção categorias HIVAIDS AIDS HIV/AIDS gerais saudável emocional sistemas multimídia impresso oficinasworkshops oficinas workshops oficinas/workshops farmacológico 20 201 2

Abstract Objective To map the scientific production on educational strategies and the content covered in the education of people living with HIV. Methods This is a scoping review in which the selection of articles was carried out in April 2021 and updated in October 2022 in ten data sources; the review followed the assumptions established by the Joanna Briggs Institute and the checklist of Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews. The results were descriptively analyzed and summarized in a chart. Results A total of 17 studies were selected, predominantly published in 2017; The USA and Brazil were the countries with the largest number of productions. Most studies sought to evaluate the impact and effectiveness of strategies and develop or validate health education instruments and prevention activities. Concerning the content covered by the strategies, five categories were formed: initial guidance on HIV/AIDS, general care, healthy living, sexual health, and emotional support. The educational strategies that stood out concerning greater patient adherence to treatment are related to the development of systems, programs, and multimedia. The booklets promoted empowerment and autonomy for people living with HIV. Conclusion The main educational strategies were mapped, with emphasis on booklets, printed material, multimedia resources, systems, forms, and workshops, covering initial guidance on HIV/AIDS, pharmacological treatment, general care, healthy living, sexual health, and social and emotional support. Open Science Framework (OSF): https://osf.io/754uk/?view_only=6491865a3d12424d81af2c4099c112c3 HIV 202 sources MetaAnalyses Meta Analyses Reviews chart 1 selected 2017 productions activities formed HIVAIDS AIDS HIV/AIDS care support systems programs mapped material resources forms workshops OSF (OSF) https//osf.io/754uk/view_only=6491865a3d12424d81af2c4099c112c3 httpsosfio754ukviewonly6491865a3d12424d81af2c4099c112c3 httpsosfioukviewonlyaddafccc https //osf.io/754uk/ view_only=6491865a3d12424d81af2c4099c112c3 osf io 754uk view only 6491865a3d12424d81af2c4099c112c3 uk d af c https://osf.io/754uk/?view_only=6491865a3d12424d81af2c4099c112c 20 201 (OSF https//osf.io/754uk/view_only=6491865a3d12424d81af2c4099c112c httpsosfio ukviewonly httpsosfio754ukviewonly6491865a3d12424d81af2c4099c112c osfio754uk osfiouk //osf.io/754uk viewonly6491865a3d12424d81af2c4099c112c3 viewonlyaddafccc view_only=6491865a3d12424d81af2c4099c112c addafccc 6491865a3d12424d81af2c4099c112c 2 osfio viewonly viewonly6491865a3d12424d81af2c4099c112c

Resumen Objetivo Mapear la producción científica sobre las estrategias educativas y los contenidos abordados en la educación de personas que viven con el VIH. Métodos Esta es una revisión de alcance, cuya selección de artículos se realizó en abril de 2021 y se actualizó en octubre de 2022 en diez fuentes de datos. La revisión siguió las premisas establecidas por el Joanna Briggs Institute y la checklist de los Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews. Los resultados fueron analizados descriptivamente y sintetizados en un cuadro. Resultados Se seleccionaron 17 estudios con publicación predominante en 2017. Estados Unidos de América y Brasil fueron los países con mayor cantidad de producciones. La mayoría de los estudios buscó evaluar el impacto y la eficacia de las estrategias y elaborar o validar instrumentos de educación para la salud y actividades de prevención. Con relación al contenido abordado por las estrategias, se formaron cinco categorías: instrucciones iniciales sobre VIH/SIDA, cuidados generales, vida saludable, salud sexual y apoyo emocional. Las estrategias educativas que se destacaron con relación a una mayor adhesión de los pacientes al tratamiento están relacionadas con el desarrollo de sistemas, programas y multimedia. Las cartillas promovieron empoderamiento y autonomía de personas que viven con el VIH. Conclusión Se mapearon las principales estrategias educativas, con énfasis en cartillas, material impreso, recursos multimedia, sistemas, formularios y talleres/workshops, que abordaron instrucciones iniciales sobre VIH/SIDA, tratamiento farmacológico, cuidados generales, vida saludable, salud sexual y apoyo social y emocional. Open Science Framework (OSF): https://osf.io/754uk/?view_only=6491865a3d12424d81af2c4099c112c3 VIH alcance 202 datos MetaAnalyses Meta Analyses Reviews cuadro 1 2017 producciones prevención categorías VIHSIDA SIDA VIH/SIDA generales saludable emocional sistemas multimedia impreso talleresworkshops talleres workshops talleres/workshops farmacológico OSF (OSF) https//osf.io/754uk/view_only=6491865a3d12424d81af2c4099c112c3 httpsosfio754ukviewonly6491865a3d12424d81af2c4099c112c3 httpsosfioukviewonlyaddafccc https //osf.io/754uk/ view_only=6491865a3d12424d81af2c4099c112c3 osf io 754uk view only 6491865a3d12424d81af2c4099c112c3 uk d af c https://osf.io/754uk/?view_only=6491865a3d12424d81af2c4099c112c 20 201 (OSF https//osf.io/754uk/view_only=6491865a3d12424d81af2c4099c112c httpsosfio ukviewonly httpsosfio754ukviewonly6491865a3d12424d81af2c4099c112c osfio754uk osfiouk //osf.io/754uk viewonly6491865a3d12424d81af2c4099c112c3 viewonlyaddafccc view_only=6491865a3d12424d81af2c4099c112c addafccc 6491865a3d12424d81af2c4099c112c 2 osfio viewonly viewonly6491865a3d12424d81af2c4099c112c

Abstract Background: The relationship between viral infections and host factors holds high hopes for identifying the role of Interferon Lambda 3 (IFNL3) and Interleukin 6 (IL-6) polymorphisms in the development of Chronic Liver Disease (CLD) in patients infected with hepatitis Delta virus (HDV) in the Western Brazilian Amazon. Methods: Cross-sectional study conducted with a cohort of 40 chronic HDV patients, 27 with CLD and 13 without evident liver damage. Biological samples from the participants were analyzed using the polymerase chain reaction (PCR) technique, followed by sequencing by the automated Sanger method. Results: The rs8099917 T allele, from the IFNL3 gene, showed a higher frequency in both groups; however, it was not possible to establish an association with HDV infection [OR = 1.42 (0.42 - 4.75; p = 0.556 (95% CI). For IL-6, the rs1800795 G allele was superior to rs1800795 C. Analyzing both distributions in the studied groups, any association with HDV was absent (p > 0.05). Conclusion: The results suggest that the rs8099917 T/G (IFNL3) and rs1800795 G/C (IL-6) polymorphisms are not associated with the evolution of HDV in the studied population. Background IFNL (IFNL3 IL6 IL (IL-6 (CLD (HDV Amazon Methods Crosssectional Cross sectional 4 2 1 damage PCR (PCR technique method Results rs rs809991 gene groups however OR 142 42 1.4 0.42 042 0 (0.4 4.75 475 75 0556 556 0.55 95% 95 (95 CI. CI . CI) IL6, 6, IL-6 rs180079 C 0.05. 005 0.05 05 0.05) Conclusion TG GC population (IFNL (IL- rs80999 14 1. 0.4 04 (0. 4.7 47 7 055 55 0.5 9 (9 IL- rs18007 00 0.0 (IL rs8099 0. (0 4. 5 ( rs1800 rs809 rs180 rs80 rs18 rs8 rs1

Abstract Background Enteropathic spondyloarthritis is underdiagnosed and inflammatory biomarkers and ultrasonography (US) could be useful for screening inflammatory bowel disease (IBD) patients. The objective of this study was to evaluate the prevalence of spondyloarthritis (SpA) in IBD patients, according to the Assessment of SpondyloArthritis International Society (ASAS) criteria and the correlation of results of US of entheses and joints with plasma calprotectin levels. Methods This was an observational cross-sectional study. Patients from the IBD outpatient clinic of a reference center were evaluated according to ASAS criteria classification, results of US of entheses and joints, and inflammatory biomarker measurements (erythrocyte sedimentation rates, C-reactive protein levels, fecal and plasma calprotectin levels). A p value lower than 0.05 was considered significant. Results A total of 30.5% of the studied sample (n = 118) of patients with IBD presented at least one inflammatory musculoskeletal manifestation. The overall prevalence of enteropathic SpA was 13.55%, with 10.16% axial SpA and 4.23% peripheral SpA according to the ASAS criteria. A total of 42.1% of patients had an MASEI score greater than 18, 35.2% had synovitis, and 14.7% had tenosynovitis on US, increasing the frequency of diagnosis of entero- pathic SpA to 22.8%. Plasma calprotectin levels were similar to those in healthy controls, and correlated only with the fecal calprotectin level (p 0.041). Conclusions A total of 13.5% of patients met the criteria in accordance with the ASAS criteria for enteropathic SpA, which increased to 22.8% with the addition of US. The prevalence of enthesitis, synovitis and tenosynovitis by US of symptomatic joints and entheses were 42%, 35% and 14.7% respectively. Plasma calprotectin was correlated with fecal calprotectin but not with inflammatory biomarkers or US or ASAS criteria. (US (IBD (SpA (ASAS crosssectional cross sectional classification erythrocyte rates Creactive C reactive . levels) 005 0 05 0.0 significant 305 30 5 30.5 n 118 manifestation 1355 13 55 13.55% 1016 10 16 10.16 423 4 23 4.23 421 42 1 42.1 18 352 35 2 35.2 147 14 7 14.7 entero 228 22 8 controls 0.041. 0041 0.041 041 0.041) 135 13.5 22.8 enthesitis 42% respectively 00 0. 3 30. 11 13.55 101 10.1 4.2 42. 35. 14. 004 0.04 04 13. 22. 10. 4.

ABSTRACT Objectives: Here, we investigated the effects of hyperventilation on acute lung injury (ALI) in spontaneously breathing rats. Methods: Wistar rats were randomized to receive either intraperitoneal lipopolysaccharides (LPS) or saline, and intravenous infusion of NH4Cl (to induce metabolic acidosis and hyperventilation) or saline. Four groups were established: control-control (C-C), control-hyperventilation (C-HV), LPS-control (LPS-C), and LPS-hyperventilation (LPS-HV). Venous blood gases were collected before and after NH4Cl infusion and analyzed to confirm the presence of metabolic acidosis and hyperventilation. After euthanasia, lung injury was assessed using the ALI score, morphometric quantification of perivascular edema, neutrophil counts in the bronchoalveolar lavage, and mRNA expression of biological markers in the lung tissue. Results: Hyperventilation induced inflammatory lung injury in previously healthy lungs and exacerbated injuries previously induced by LPS (ALI score: C-C=0.14 [IQR 0.12; 0.14]; C-HV=0.36 [IQR 0.31; 0.37]; LPS-C=0.51 [IQR 0.50; 0.54]; LPS-HV=0.58 [IQR 0.56; 0.62]; p<0.01). Perivascular edema, neutrophil counts in bronchoalveolar lavage, and amphiregulin mRNA expression were higher in the LPS-HV group compared to the control group. Conclusions: Hyperventilation increased inflammatory injury in rats with ALI during spontaneous ventilation. These results suggest that the impact of vigorous spontaneous breathing efforts on worsening inflammatory lung injury warrants further investigation. Objectives Here Methods (LPS saline NHCl NH Cl established controlcontrol CC, CC C , (C-C) controlhyperventilation CHV, CHV HV (C-HV) LPScontrol LPSC, LPSC (LPS-C) LPShyperventilation LPSHV. LPSHV . (LPS-HV) euthanasia score edema lavage tissue Results CC=0.14 CC014 C=0.14 0 14 C-C=0.1 IQR 0.12 012 12 0.14 014 0.14] CHV=0.36 CHV036 HV=0.36 36 C-HV=0.3 0.31 031 31 0.37 037 37 0.37] LPSC=0.51 LPSC051 C=0.51 51 LPS-C=0.5 0.50 050 50 0.54 054 54 0.54] LPSHV=0.58 LPSHV058 HV=0.58 58 LPS-HV=0.5 0.56 056 56 0.62 062 62 0.62] p<0.01. p001 p p<0.01 01 p<0.01) Conclusions ventilation investigation (C-C (C-HV (LPS-C (LPS-HV CC=0.1 CC01 C014 C=0.1 1 C-C=0. 0.1 CHV=0.3 CHV03 HV036 HV=0.3 3 C-HV=0. 0.3 03 LPSC=0.5 LPSC05 C051 C=0.5 5 LPS-C=0. 0.5 05 LPSHV=0.5 LPSHV05 HV058 HV=0.5 LPS-HV=0. 0.6 06 6 p00 p<0.0 CC=0. CC0 C01 C=0. C-C=0 0. CHV=0. CHV0 HV03 HV=0. C-HV=0 LPSC=0. LPSC0 C05 LPS-C=0 LPSHV=0. LPSHV0 HV05 LPS-HV=0 p0 p<0. CC=0 C0 C=0 C-C= CHV=0 HV0 HV=0 C-HV= LPSC=0 LPS-C= LPSHV=0 LPS-HV= p<0 CC= C= C-C CHV= HV= C-HV LPSC= LPS-C LPSHV= p<

RESUMO Objetivo: Avaliar o efeito de diferentes materiais restauradores para uso em CAD-CAM na resistência à flexão biaxial após envelhecimento mecânico e térmico acelerado. Métodos: Foram fabricadas amostras (n=10) por grupo: CL (cerâmica reforçada com leucita: IPS Empress CAD) e NR (resina nanocerâmica: Lava Ultimate). A análise morfológica da superfície foi realizada por Microscopia Eletrônica de Varredura, e a análise química foi realizada por Espectroscopia de Raios X por Dispersão de Energia em uma amostra de cada grupo. Todas as amostras foram submetidas a envelhecimento acelerado, inicialmente através de ensaio de fadiga utilizando máquina mecânica de carregamento cíclico (200.000 ciclos, força 15N, 1Hz) em água destilada à temperatura (37°C). O envelhecimento térmico foi realizado em termociclador (5.000 ciclos, banhos de 5°C-55°C). Resultados: A análise estatística 1 Fator Anova (p<0,05) O grupo CL apresentou superfície mais difusa e quimicamente apresentou presença de Silício, Oxigênio, Alumínio, Sódio, Potássio e Cálcio. O grupo NR apresentava superfície mais compacta e continha os seguintes elementos químicos: Oxigênio, Silício, Carbono, Zircônio, Nitrogênio, Alumínio e Sódio. O CL (193,1MPa) apresentou valor de resistência mecânica superior ao NR (52,45MPa), o que foi estatisticamente significativo (p=0,000). Porém, o NR (2,3) apresentou menor média de fragmentos após fratura em relação ao CL (5,6). Conclusão: Os materiais restauradores exibiram morfologia e composição química distintas. Esses materiais restauradores tiveram um efeito estatisticamente significativo na resistência à flexão biaxial, sendo a cerâmica reforçada com leucita mecanicamente superior à resina nanocerâmica. E a família de resinas de matriz cerâmica apresentou menor quantidade de fragmentos após fratura em comparação ao material representativo da família vitrocerâmica. Objetivo CADCAM CAD CAM acelerado Métodos n=10 n10 n 10 (n=10 nanocerâmica Ultimate. Ultimate . Ultimate) Varredura 200.000 200000 200 000 (200.00 ciclos 15N N 1Hz Hz 37°C. 37C C 37°C 37 (37°C) 5.000 5000 5 (5.00 5°C55°C. 5C55C CC 5°C 55°C 55 5°C-55°C) Resultados p<0,05 p005 p 0 05 (p<0,05 Silício Oxigênio Sódio Cálcio químicos Carbono Zircônio Nitrogênio 193,1MPa 1931MPa MPa 193 1MPa (193,1MPa 52,45MPa, 5245MPa 52,45MPa , 52 45MPa (52,45MPa) p=0,000. p0000 p=0,000 (p=0,000) Porém 2,3 23 2 3 (2,3 5,6. 56 5,6 6 (5,6) Conclusão distintas vitrocerâmica n=1 n1 (n=1 200.00 20000 20 00 (200.0 (37°C 5.00 500 (5.0 C55 5°C55°C 5C 55C 5°C-55°C p<0,0 p00 (p<0,0 19 (52,45MPa p000 p=0,00 (p=0,000 2, (2, 5, (5,6 n= (n= 200.0 2000 (200. 5.0 50 (5. C5 p<0, p0 (p<0, p=0,0 (p=0,00 (2 (5, (n 200. (200 5. (5 p<0 (p<0 p=0, (p=0,0 ( (20 p< (p< p=0 (p=0, (p p= (p=0 (p=

ABSTRACT Objective: The aim of this study was to evaluate the effect of different restorative materials for use in CAD-CAM on biaxial flexural strength after accelerated mechanical and thermal aging. Methods: Samples were fabricated and divided into two groups: CL (leucite-reinforced ceramic: IPS Empress CAD) and NR (nanoceramic resin: Lava Ultimate). Morphological analysis of the surface was performed using Scanning Electron Microscopy, and chemical analysis was conducted using Energy Dispersive X-ray Spectroscopy on one sample from each group. All samples underwent accelerated aging, initially through fatigue testing using a mechanical cyclic loading machine (200,000 cycles, 15N force, 1Hz rotation speed) in distilled water at room temperature (37°C). Subsequently, thermal aging was carried out in a thermocycler (5,000 cycles, baths at 5°C and 55°C). Results: The CL group exhibited a more diffuse surface, and chemically, it showed the presence of Silicon, Oxygen, Aluminum, Sodium, Potassium, and Calcium. The NR group had a more compact surface and contained the following chemical elements: Oxygen, Silicon, Carbon, Zirconium, Nitrogen, Aluminum, and Sodium. The CL (193.1MPa) showed a higher mechanical strength value than the NR (52.45MPa), which was statistically significant. However, the NR (2.3) exhibited a lower average number of fragments after fracture compared to the CL (5.6). Conclusion: Restorative materials exhibited distinct morphology and chemical composition. These restorative materials had a statistically significant effect on biaxial flexural strength, with leucite-reinforced ceramics being mechanically superior to nanoceramic resin. And the ceramic matrix resin family showed a lower quantity of fragments after fracture compared to the material representing the glass-ceramic family. Objective CADCAM CAD CAM Methods groups leucitereinforced leucite reinforced Ultimate. Ultimate . Ultimate) Microscopy Xray X ray 200,000 200000 200 000 (200,00 cycles N force Hz speed 37°C. 37C C 37°C 37 (37°C) Subsequently 5,000 5000 5 (5,00 5C 55°C. 55C 55°C 55 55°C) Results chemically Silicon Oxygen Aluminum Sodium Potassium Calcium elements Carbon Zirconium Nitrogen 193.1MPa 1931MPa MPa 193 1MPa (193.1MPa 52.45MPa, 5245MPa 52.45MPa , 52 45MPa (52.45MPa) However 2.3 23 2 3 (2.3 5.6. 56 5.6 6 (5.6) Conclusion composition glassceramic glass 200,00 20000 20 00 (200,0 (37°C 5,00 500 (5,0 19 (52.45MPa 2. (2. 5. (5.6 200,0 2000 0 (200, 5,0 50 (5, 1 (2 (5. 200, (200 5, (5 ( (20

RESUMO A tipologia da estrutura é um indicador composto que compreende a estrutura física, a disponibilidade de equipamentos, recursos materiais e humanos, além dos sistemas de informação. Com o objetivo de desenvolver uma tipologia para avaliar as Unidades Básicas de Saúde (UBS) do Distrito Federal (DF), construiu-se um instrumento com 43 variáveis, a partir da proposta de tipologia nacional. Entre agosto/2020 e janeiro/2021, coletaram-se dados nas UBS e nos sistemas de informação do DF. Na análise, ponderando as dimensões, gerou-se a classificação nos tipos A, B, C, D e E (A adequada/E insuficiente). Das 165 UBS existentes, 157 forneceram dados completos para avaliação. Três UBS obtiveram classificação Tipo A, máxima, representando 1,9% do total; 53 UBS do Tipo B (33,8%); 52 do Tipo C (33,1%); 30 do Tipo D (19,1%) e 19 do Tipo E (12,1%). Nove UBS apresentaram pontuação muito baixa em ‘serviços disponíveis’, expondo indisponibilidade de vacinação, teleatendimento, exames, medicamentos e incompletude de equipes. O resultado evidenciou que as UBS do DF necessitam de melhorias estruturais, que, em consonância com as demais ações previstas no Programa Qualis-APS, contribuirá para a melhoria da qualidade da atenção primária no DF e o desenvolvimento do pleno potencial das equipes de saúde. física equipamentos humanos (UBS DF, , (DF) construiuse construiu se 4 variáveis nacional agosto2020 agosto 2020 agosto/202 janeiro2021 janeiro 2021 janeiro/2021 coletaramse coletaram análise dimensões gerouse gerou adequadaE adequada insuficiente. insuficiente . insuficiente) 16 existentes 15 avaliação máxima 1 9 1,9 total 5 33,8% 338 33 8 (33,8%) 33,1% 331 (33,1%) 3 19,1% 191 (19,1% 12,1%. 121 12,1% 12 (12,1%) serviços disponíveis, disponíveis disponíveis’ vacinação teleatendimento exames estruturais QualisAPS, QualisAPS Qualis APS, APS Qualis-APS saúde (DF agosto202 202 agosto/20 janeiro202 janeiro/202 1, 33,8 (33,8% 33,1 (33,1% 19,1 (19,1 12,1 (12,1% agosto20 20 agosto/2 janeiro20 janeiro/20 33, (33,8 (33,1 19, (19, 12, (12,1 agosto2 2 agosto/ janeiro2 janeiro/2 (33, (19 (12, janeiro/ (33 (1 (12 (3 (

ABSTRACT The structure typology is a composite indicator that includes physical structure, availability of equipment, material and human resources, and information systems. A 43-variable instrument based on the proposed national typology was constructed to develop a typology to evaluate the Primary Health Care Units (PHC Units) in the Federal District (FD), Brazil. Data were collected from the PHC Unit and the FD’s information systems from August/2020 to January/2021. In the analysis, the dimensions were weighted and classified into types A, B, C, D, and E (A adequate/E insufficient)-one hundred fifty-seven of the 165 existing PHC Units provided complete data for evaluation. Three PHC Units obtained the maximum Type A classification, representing 1.9% of the total; 53 PHC Units were Type B (33.8%), 52 Type C (33.1%), 30 Type D (19.1%), and 19 Type E (12.1%). Nine PHC Units scored very low in ‘available services’ with unavailable vaccinations, telehealth, tests, and medicines and had incomplete teams. The result showed that the PHC Units in the FD require structural improvements, which, aligned with the other actions provided for in the Qualis-APS Program, will improve PHC quality in the FD and develop the full potential of the health teams. equipment resources 43variable variable 43 FD, , (FD) Brazil FDs s August2020 August 2020 August/202 January2021 January 2021 January/2021 analysis adequateE adequate insufficientone insufficient one fiftyseven fifty seven 16 evaluation classification 1 9 1.9 total 5 33.8%, 338 33.8% 33 8 (33.8%) 33.1%, 331 33.1% (33.1%) 3 19.1%, 191 19.1% (19.1%) 12.1%. 121 12.1% . 12 (12.1%) available services vaccinations telehealth tests teams improvements which QualisAPS Qualis APS Program 4 (FD August202 202 August/20 January202 January/202 1. 33.8 (33.8% 33.1 (33.1% 19.1 (19.1% 12.1 (12.1% August20 20 August/2 January20 January/20 33. (33.8 (33.1 19. (19.1 12. (12.1 August2 2 August/ January2 January/2 (33. (19. (12. January/ (33 (19 (12 (3 (1 (

Resumo Hypostomus está distribuído por bacias da América Central e do Sul, com grande diversidade de espécies com conflitos taxonômicos. Desta forma, a integração de técnicas auxiliares contribui para a compreensão da sistemática e filogenia do grupo. Assim, este estudo teve como objetivo investigar Hypostomus cochliodon e H. boulengeri do riacho Onça (bacia do rio Paraguai) por meio de técnicas aloenzimáticas e citogenéticas. As análises citogenéticas em H. boulengeri mostraram número cromossômico igual a 2n = 68 (14m+14sm+18st+22a), sistema de NOR múltiplo revelado por Ag-NOR e 18S-FISH, um polimorfismo de heterocromatina em acrocêntricos e a presença de microcromossomos Bs. Hypostomus cochliodon mostrou um número diploide de 64 cromossomos (16m+26sm+14st+8a); apesar do sistema de NOR simples, alguns indivíduos apresentaram NOR em ambos os telômeros detectados por Ag-NOR e 18S-FISH. Uma isozima identificou dois loci diagnósticos (Idh-A and Gdh-A) entre as duas espécies e múltiplos loci com alelos únicos em H. boulengeri. A variabilidade genética indicada pela heterozigosidade média (He) foi de 0,2461 e 0,0309 em H. boulengeri e H. cochliodon, respectivamente. Assim, este estudo relata os primeiros dados citogenéticos para H. boulengeri e os primeiros dados isoenzimáticos para H. boulengeri e H. cochliodon. As duas espécies apresentaram evidentes diferenças citogenéticas e isoenzimáticas com a obtenção de marcadores genéticos exclusivos fornecendo suporte para futuros estudos evolutivos no grupo. Sul taxonômicos forma grupo Assim H bacia Paraguai n 6 14m+14sm+18st+22a, 14m14sm18st22a msmsta 14m+14sm+18st+22a , 14m 14sm 18st 22a m sm st (14m+14sm+18st+22a) AgNOR Ag 18SFISH, 18SFISH SFISH 18S FISH, FISH S 18S-FISH Bs 16m+26sm+14st+8a 16m26sm14st8a 16m 26sm 14st 8a (16m+26sm+14st+8a) simples 18SFISH. FISH. IdhA Idh GdhA Gdh Gdh-A He (He 02461 0 2461 0,246 00309 0309 0,030 respectivamente (14m+14sm+18st+22a (16m+26sm+14st+8a 0246 246 0,24 0030 030 0,03 024 24 0,2 003 03 0,0 02 2 0, 00

Abstract Hypostomus is distributed by Central and South America basins, with diverse species with taxonomic conflicts. This way, the integration of auxiliary techniques contributes to understanding the systematics and phylogeny of the group. Thus, this study aimed to investigate the Hypostomus cochliodon and H. boulengeri from the Onça stream (Paraguai River basin) by allozyme and cytogenetic techniques. Hypostomus boulengeri showed a diploid number of 68 chromosomes (14m+14sm+18st+22a), multiple NOR revealed by Ag-NOR and 18S rDNA FISH, a polymorphism of heterochromatin in acrocentrics and the presence of B microchromosome. Hypostomus cochliodon showed a diploid number of 64 chromosomes (16m+26sm+14st+8a); despite the single NOR, some individuals showed NOR in both telomeres detected by Ag-NOR and 18S rDNA FISH. Isozyme identified two diagnostic loci (Idh-A and Gdh-A) between the two species and multiple loci with unique alleles in H. boulengeri. The genetic variability indicated by the mean heterozygosity (He) was 0.2461 and 0.0309 in H. boulengeri and H. cochliodon,respectively.Thus, this study reports the first cytogenetic data for H. boulengeri and the first isozymatic data for H. boulengeri and H. cochliodon. The two species presented evident cytogenetic and isoenzymatic differences with the obtaining of exclusive genetic markers providing support for future evolutionary studies in the group. basins conflicts way group Thus H Paraguai basin 6 14m+14sm+18st+22a, 14m14sm18st22a msmsta 14m+14sm+18st+22a , 14m 14sm 18st 22a m sm st (14m+14sm+18st+22a) AgNOR Ag S FISH microchromosome 16m+26sm+14st+8a 16m26sm14st8a 16m 26sm 14st 8a (16m+26sm+14st+8a) IdhA Idh A GdhA Gdh Gdh-A He (He 02461 0 2461 0.246 00309 0309 0.030 cochliodonrespectivelyThus respectively cochliodon,respectively.Thus (14m+14sm+18st+22a (16m+26sm+14st+8a 0246 246 0.24 0030 030 0.03 024 24 0.2 003 03 0.0 02 2 0. 00

Abstract Several materials have been developed to preserve pulp vitality. They should have ideal cytocompatibility characteristics to promote the activity of stem cells of human exfoliated deciduous teeth (SHED) and thus heal pulp tissue. Objective To evaluate the cytotoxicity of different dilutions of bioceramic material extracts in SHED. Methodology SHED were immersed in αMEM + the material extract according to the following experimental groups: Group 1 (G1) –BBio membrane, Group 2 (G2) - Bio-C Repair, Group 3 (G3) - MTA Repair HP, Group 4 (G4) – TheraCal LC, and Group 5 (G5) - Biodentine. Positive and negative control groups were maintained respectively in αMEM + 10% FBS and Milli-Q Water. The methods to analyze cell viability and proliferation involved MTT and Alamar Blue assays at 24, 48, and 72H after the contact of the SHED with bioceramic extracts at 1:1 and 1:2 dilutions. Data were analyzed by the three-way ANOVA, followed by Tukey’s test (p<0.05). Results At 1:1 dilution, SHED in contact with the MTA HP Repair extract showed statistically higher cell viability than the other experimental groups and the negative control (p<0.05), except for TheraCal LC (p> 0.05). At 1:2 dilution, BBio Membrane and Bio-C showed statistically higher values in intra- and intergroup comparisons (p<0.05). BBio Membrane, Bio-C Repair, and Biodentine extracts at 1:1 dilution showed greater cytotoxicity than 1:2 dilution in all periods (p<0.05). Conclusion MTA HP Repair showed the lowest cytotoxicity even at a 1:1 dilution. At a 1:2 dilution, the SHED in contact with the BBio membrane extract showed high cell viability. Thus, the BBio membrane would be a new non-cytotoxic biomaterial for SHED. Results offer possibilities of biomaterials that can be indicated for use in clinical regenerative procedures of the dentin-pulp complex. vitality (SHED tissue G1 G (G1 G2 (G2 BioC Bio C G3 (G3 G4 (G4 G5 (G5 10 MilliQ Milli Q Water 24 48 H 11 1: 12 threeway three way ANOVA Tukeys Tukey s p<0.05. p005 p p<0.05 . 0 05 (p<0.05) p<0.05, , p> (p 0.05. 005 0.05 0.05) intra Thus noncytotoxic non cytotoxic dentinpulp dentin complex (G p00 p<0.0 (p<0.05 00 0.0 p0 p<0. (p<0.0 0. p<0 (p<0. p< (p<0 (p<

Polyethylene of Raised Temperature Resistance (PE-RT) is a specially designed high-density polyethylene utilizing bimodal resin technology. It is suitable for oil and gas pipeline applications, exhibiting excellent long-term creep resistance at elevated temperatures. Flexible risers require security against potential offshore oil leaks, prompting the reinforcement of PE-RT with graphene oxide (GO) at concentrations of 0.5, 1.0, and 2.0 wt.%. The nanocomposites were processed in a twin-screw mini extruder at 180°C, with a screw speed of 100 rpm and a residence time of 6 min. The results indicated an increase in the thermal stability of the nanocomposite by 14°C. XRD and DSC analyses revealed a higher crystallinity index for the sample with the lowest GO content. The water contact angle values increased from 98° in the pure matrix to 102° in the nanocomposite, suggesting a slight increase in the material's hydrophobicity due to nanoparticle incorporation. SEM images demonstrated a good interface between GO nanoparticles and PE-RT without agglomeration. The Shore D hardness increased from 50 to 59 with 2.0 wt.% GO. Although the 0.5 wt.% GO composition exhibited a higher degree of crystallinity, other properties such as thermal resistance, Shore D hardness, contact angle, dispersion, and homogeneity tended to improve with increasing nanoparticle content. Consequently, it can be concluded that the optimal concentration of GO in the PE-RT matrix is approximately 1.0 wt.%. Incorporating 1.0 wt.% GO resulted in an increase in Young's modulus from 335 ± 37 MPa to 409 ± 42 MPa, indicating enhanced mechanical strength of PE-RT. Finally, aging tests showed that GO reduced the swelling degree of the final material, suggesting that PE-RT/GO is a promising candidate for pipeline applications. PERT PE RT (PE-RT highdensity high density technology applications longterm long term temperatures leaks (GO 05 0 5 10 1 20 2 2. wt twinscrew twin 180C C 180 180°C min 14C 14 14°C content 98 102 materials material s incorporation agglomeration wt. 0. dispersion Consequently 1. Youngs Young 33 3 40 4 PERT. RT. Finally PERT/GO PERTGO RT/GO 18 9 RTGO

ABSTRACT Dengue is a disease with a high-impact on public health worldwide. Many researches have focused on the cell receptors involved in its pathogenesis. The role of soluble isoforms of DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) receptor in the process of Dengue Virus (DENV) infection is not well understood. This work proposes to evaluate changes in the infection process of Immature Dendritic Cells (iDCs) by DENV in the presence of DC-SIGN recombinant soluble isoforms 8, 10, and 12. The recombinant isoforms were built by heterologous expression, the DENV-2 was multiplied in the Aedes albopictus C6/36 cells and quantified in BHK-21 cells, and the iDCs were produced from the THP-1 strain. Infection assays were performed in the presence of iDCs, DENV-2, and isoforms 8, 10, and 12 separately at 25, 50 and 100 ng/mL. The final viral load was estimated by qPCR and statistical analysis was performed by Kruskal-Wallis and ANOVA tests. The iDC profile was confirmed by increasing expression of CD11c, CD86, and CD209 surface markers and maintaining CD14 expression. Infection assays demonstrated a 23-fold increase in DENV viral load in the presence of isoforms 8 and 10 at 100 ng/mL compared to the viral control (p < 0.05), while isoform 12 did not alter the viral load. It was possible to conclude that at 100 ng/mL isoforms (8 and 10) can interact with DENV, increasing viral infection, and potentially acting as opsonins. highimpact high impact worldwide pathogenesis DCSIGN DC SIGN CellSpecific Cell Specific ICAM3 ICAM 3 ICAM- Nonintegrin Non integrin Non-integrin (DENV understood (iDCs DENV2 2 DENV- C636 C C6 36 C6/3 BHK21 BHK 21 BHK-2 THP1 THP 1 THP- strain DENV2, 2, 25 5 ngmL ng mL KruskalWallis Kruskal Wallis tests CD11c CDc CD c CD86 CD20 CD1 23fold fold 23 p 0.05, 005 0.05 , 0 05 0.05) ( opsonins C63 C6/ BHK2 BHK- CD8 CD2 00 0.0 0.