Diaporthe phaseolorum, an endophyte isolated from Combretum lanceolatum, has exhibited diverse biological activities including antimicrobial, leishmanicidal, antitumoral, and herbicidal properties. Regardless of these activities, there remains limited research on its chemical constituents. Therefore, this study aimed to investigate the metabolome of D. phaseolorum employing mass spectrometry data dereplication through molecular networking and MolNetEnhancer tools. Twenty two molecular families were annotated, including prenol lipids, benzenoids, organoheterocyclic compounds, and nucleosides. Among the 41 nodes annotated, 36 metabolites were confirmed via spectral references, including alismol, farnesol, linoleic acid, tyramine, N-acetyl-tyramine, 5-methoxy-1H-indole-3-carbaldehyde, 6-pentyl-2H-pyran-2-one, and adenosine. Further node annotation was achieved through MolNetEnhancer, resulting in the classification of an additional 68 nodes into various molecular families. Consequently, this study represents a significant contribution to the identification of the D. phaseolorum metabolome, highlighting its secondary metabolites and providing valuable chemical insights to elucidate its demonstrated biological activities. lanceolatum antimicrobial leishmanicidal antitumoral properties constituents Therefore D tools annotated lipids benzenoids compounds nucleosides 4 3 references alismol farnesol acid tyramine Nacetyltyramine, Nacetyltyramine N acetyl N-acetyl-tyramine 5methoxy1Hindole3carbaldehyde, 5methoxy1Hindole3carbaldehyde methoxyHindolecarbaldehyde 5 methoxy 1H indole carbaldehyde, carbaldehyde H 5-methoxy-1H-indole-3-carbaldehyde 6pentyl2Hpyran2one, 6pentyl2Hpyran2one pentylHpyranone 6 pentyl 2H pyran 2 one, one 6-pentyl-2H-pyran-2-one adenosine Consequently Hindole Hpyran

ABSTRACT Introduction Chemotherapy and radiation therapy are considered standard treatments for stage II seminoma patients; however, these therapies are associated with long-term toxicities. Recently, retroperitoneal lymph node dissection has emerged as an alternative strategy, and the first three phase II trials were published in 2023 with promising results. The present study conducted a systematic review and meta-analysis to evaluate this surgery as an alternative treatment for stage IIA/B seminoma patients. Purpose Seminomas are the most common testicular tumors, often affecting young adult males. Standard treatments for stage II seminomas include chemotherapy and radiation therapy, but these therapies are associated with long-term toxicities. Thus, identifying alternative strategies is paramount. Herein, we conducted a systematic review and meta-analysis to appraise the efficacy and safety of retroperitoneal lymph node dissection (RPLND) for treating this condition. Methods We systematically searched the PubMed, Embase, and Cochrane databases for studies evaluating RPLND as a primary treatment for stage II A/B seminomas. Using a random-effects model, single proportion and means and pooled 2-year recurrence-free survival rates with hazard rates and 95% CI were calculated. Results Seven studies were included, comprising 331 males with stage II seminomas. In the pooled analysis, the recurrence rate was 17.69% (95% CI 12.31–24.75), and the 2-year RFS rate was 81% (95% CI 0.77–0.86). The complication rate was 9.16% (95% CI 6.16–13.42), the Clavien–Dindo > 2 complication rate was 8.83% (95% CI 5.76–13.31), and the retrograde ejaculation rate was 7.01% (95% CI 3.54–13.40). The median operative time was 174.68 min (95% CI 122.17–249.76 min), median blood loss was 105.91 mL (95% CI 46.89–239.22 mL), and patients with no evidence of lymph node involvement ranged from 0–16%. Conclusions Primary RPLNDs for treating stage IIA/B seminomas have favorable RFS rates, with low complication and recurrence rates. These findings provide evidence that this surgery is a viable alternative therapy for these patients. however longterm long term toxicities Recently strategy 202 results metaanalysis meta analysis IIAB IIA B tumors Thus paramount Herein (RPLND condition PubMed Embase AB A randomeffects random effects model 2year year recurrencefree free 95 calculated included 33 1769 17 69 17.69 (95 12.31–24.75, 12312475 12.31–24.75 , 12 31 24 75 12.31–24.75) 81 0.77–0.86. 077086 0.77–0.86 . 0 77 86 0.77–0.86) 916 9 16 9.16 6.16–13.42, 6161342 6.16–13.42 6 13 42 6.16–13.42) ClavienDindo Clavien Dindo 883 8 83 8.83 5.76–13.31, 5761331 5.76–13.31 5 76 5.76–13.31) 701 7 01 7.01 3.54–13.40. 3541340 3.54–13.40 3 54 40 3.54–13.40) 17468 174 68 174.6 1221724976 122 249 122.17–249.7 min, min) 10591 105 91 105.9 468923922 46 89 239 22 46.89–239.2 mL, mL) 016 0–16% 20 176 1 17.6 (9 1231247 12.31–24.7 07708 0.77–0.8 9.1 616134 6.16–13.4 4 88 8.8 576133 5.76–13.3 70 7.0 354134 3.54–13.4 1746 174. 122172497 122.17–249. 1059 10 105. 46892392 23 46.89–239. 0–16 17. ( 123124 12.31–24. 0770 0.77–0. 9. 61613 6.16–13. 8. 57613 5.76–13. 7. 35413 3.54–13. 12217249 122.17–249 4689239 46.89–239 0–1 12312 12.31–24 077 0.77–0 6161 6.16–13 5761 5.76–13 3541 3.54–13 1221724 122.17–24 468923 46.89–23 0– 1231 12.31–2 07 0.77– 616 6.16–1 576 5.76–1 354 3.54–1 122172 122.17–2 46892 46.89–2 123 12.31– 0.77 61 6.16– 57 5.76– 35 3.54– 12217 122.17– 4689 46.89– 12.31 0.7 6.16 5.76 3.54 1221 122.17 468 46.89 12.3 0. 6.1 5.7 3.5 122.1 46.8 12. 6. 5. 3. 122. 46.

Resumo A brucelose, uma doença causada por bactérias do gênero Brucella, apresenta uma ampla gama de sintomas em seres humanos, o que torna o diagnóstico desafiador, especialmente quando ocorrem manifestações atípicas. Este artigo relata um caso raro de espodilodiscite e ressalta a importância de investigar a brucelose em pacientes com espodilodiscite prolongada que não respondem aos tratamentos antibióticos convencionais. Além disso, destaca a necessidade de uma análise epidemiológica e radiológica completa e a realização de testes sorológicos específicos para um diagnóstico preciso da causa subjacente. A suspeita precoce, baseada em uma anamnese detalhada e em um diagnóstico cuidadoso, juntamente com os avanços nas técnicas de imagem, é crucial para o planeamento de um tratamento eficaz.

Abstract Brucellosis, a disease caused by bacteria of the genus Brucella, presents a wide range of symptoms in humans, which makes diagnosis challenging, especially when atypical manifestations occur. This article reports a rare case of spondylodiscitis and highlights the importance of investigating brucellosis in patients with prolonged spondylodiscitis who do not respond to conventional antibiotic treatments. Furthermore, it highlights the need for a complete epidemiological e radiologic analysis and the performance of specific serological tests for an accurate diagnosis of the underlying cause. Early suspicion, based on a detailed history and careful diagnosis, together with advances in imaging techniques, is crucial for planning effective treatment.

ABSTRACT BACKGROUND: Considering the ability of the health and self-management in diabetes questionnaire (HASMID-10) to verify the impact of self-management on diabetes, we highlight its relevance to scientific research and clinical applicability. However, to date, no study has been conducted to scientifically support its use in other languages. OBJECTIVE: To translate, cross-culturally adapt, and validate the HASMID-10 into the Brazilian Portuguese. DESIGN AND SETTING: A translation, cross-cultural adaptation, and validation study conducted at Ceuma University. METHODS: Study was conducted in accordance with the Guidelines for the Process of Cross-Cultural Adaptation of Self-Report Measures and Consensus-based Standards for the Selection of Health Measurement Instruments. We included participants of both sexes diagnosed with diabetes, aged between 18 and 64 years, and without cognitive deficits or any other limitations that would prevent them from answering the questionnaire. We assessed participants using the problem areas in diabetes (PAID) scale and HASMID-10. We assessed reliability using a test-retest model with a 7-day interval between assessments. We used intraclass correlation coefficient (ICC), 95% confidence interval (CI), standard error of measurement (SEM), minimum detectable difference (MDD), Spearman correlation coefficient, and floor and ceiling effects. RESULTS: Sample comprised 116 participants, most of whom were women, overweight, non-practitioners of physical activity, and nonsmokers. We observed significant correlations (P = 0.006; rho = −0.256) between the HASMID-10 and PAID, adequate reliability (ICC = 0.780) and internal consistency (Cronbach's alpha = 0.796). No ceiling or floor effects were observed. CONCLUSION: HASMID-10 has adequate measurement properties and may be used for Brazilians. BACKGROUND selfmanagement self management HASMID10 HASMID 10 (HASMID-10 applicability However date languages OBJECTIVE translate crossculturally cross culturally adapt HASMID-1 Portuguese SETTING translation crosscultural cultural adaptation University METHODS CrossCultural Cross Cultural SelfReport Self Report Consensusbased Consensus based Instruments 1 6 years PAID (PAID HASMID10. 10. testretest test retest 7day day 7 assessments ICC, ICC , (ICC) 95 CI, CI (CI) SEM, SEM (SEM) MDD, MDD (MDD) RESULTS 11 women overweight nonpractitioners non practitioners activity nonsmokers P 0.006 0006 0 006 −0.256 0256 256 0.780 0780 780 Cronbachs Cronbach s 0.796. 0796 0.796 . 796 0.796) CONCLUSION Brazilians HASMID1 (HASMID-1 HASMID- 9 (CI (SEM (MDD 0.00 000 00 −0.25 025 25 0.78 078 78 079 0.79 79 (HASMID- 0.0 −0.2 02 2 0.7 07 (HASMID 0. −0. −0 −

ABSTRACT BACKGROUND: Stroke is a major cause of mortality worldwide. Renal dysfunction is an important risk factor for stroke. Brazilian studies on stroke knowledge are generally population based. Studies stratifying stroke knowledge according to comorbidities are rare. Scientific data are essential to guide the awareness of stroke. OBJECTIVE: To assess stroke knowledge in patients with chronic kidney disease (CKD) on hemodialysis. DESIGN AND SETTING: Cross-sectional analytical study of patients with CKD on hemodialysis in north-eastern Brazil. METHODS: A self-administered questionnaire survey on stroke awareness was administered to patients with CKD on hemodialysis between April and November 2022. The chi-square test and other descriptive statistics were used. Univariate and multivariate analyses were performed using logistic regression. RESULTS: A total of 197 patients were included in the analysis. The Brazilian acronym for stroke was used by 53.5% of the participants. Less than 10.0% of the sample showed optimal decision-making ability regarding stroke. Of the participants, 29.9% knew at least one risk factor and one symptom; however, this was considered as having below the minimum capacity because they did not know the emergency service call number. In the analysis adjusted for income and education, females (odds ratio [OR], 0.40%; 95% confidence interval [CI], 0.20-0.82), older patients (OR, 0.24%; 95% CI, 0.09-0.63) and having at most one comorbidity (OR, 0.48%; 95% CI, 0.23-0.98) were factors for lower levels of knowledge or ideal decision-making capacity against stroke. CONCLUSIONS: Patients on hemodialysis, especially women and older people, have little knowledge about stroke. BACKGROUND worldwide based rare OBJECTIVE (CKD SETTING Crosssectional Cross sectional northeastern north eastern Brazil METHODS selfadministered self 2022 chisquare chi square regression RESULTS 19 535 53 5 53.5 participants 100 10 0 10.0 decisionmaking decision making 299 29 9 29.9 symptom however number education odds OR, OR , [OR] 0.40% 040 40 95 CI [CI] 0.200.82, 020082 0.20 0.82 20 82 0.20-0.82) (OR 0.24% 024 24 0.090.63 009063 0.09 0.63 09 63 0.09-0.63 0.48% 048 48 0.230.98 023098 0.23 0.98 23 98 0.23-0.98 CONCLUSIONS people 202 1 53. 10. 2 29. [OR 0.40 04 4 [CI 200 0.200.82 02008 020 0.2 082 0.8 8 0.20-0.82 0.24 02 090 0.090.6 00906 009 0.0 063 0.6 6 0.09-0.6 0.48 230 0.230.9 02309 023 098 0.9 0.23-0.9 0.4 0.200.8 0200 0. 08 0.20-0.8 0.090. 0090 00 06 0.09-0. 0.230. 0230 0.23-0. 0.200. 0.20-0. 0.090 0.09-0 0.230 0.23-0 0.200 0.20-0 0.09- 0.23- 0.20-

ABSTRACT Objective: The aim of this study was to analyze the spatiotemporal evolution of the incidence rates of human immunodeficiency virus (HIV) and acquired immune deficiency syndrome (AIDS) in the state of Paraná, Brazil. Methods: An ecological study with an analytical component of time series analysis was conducted in the state of Paraná from 2007 to 2022. The data source was the Notifiable Diseases Information System. To study the trend, the Prais–Winsten generalized linear regression model was used by decomposing the time series, and for spatial analysis, the Moran's index was applied. Results: The total sample consisted of 50,676 HIV/AIDS records. The incidence rate showed an increasing trend, with an average growth of 2.14% [95% confidence interval – 95%CI 1.16–3.13] per month. From 2007 to 2014 and from 2015 to 2022, the average number of cases in the state was 105.64 and 159.20 per 100,000 inhabitants, respectively, with significant variation among municipalities. Spatial clusters of high risk persisted in the metropolitan region, the capital, and coastal areas, and a new cluster was observed in the northern region of the state. Conclusion: The incidence rates of HIV/AIDS showed an upward trend over time. The number of cases varied considerably in some municipalities, especially in the coastal region. Spatial analysis revealed geospatial patterns of high risk in the main metropolitan areas of Paraná: Curitiba (including the coastal area), Londrina, and Maringá, which share characteristics such as a high degree of urbanization and ongoing economic development. Objective HIV (HIV AIDS (AIDS Brazil Methods 200 2022 System PraisWinsten Prais Winsten Morans Moran s applied Results 50676 50 676 50,67 HIVAIDS records 214 2 14 2.14 95% 95 [95 95CI CI 1.16–3.13 116313 1 16 3 13 month 201 10564 105 64 105.6 15920 159 20 159.2 100000 100 000 100,00 inhabitants respectively municipalities capital Conclusion including area, area , area) Londrina Maringá development 202 5067 5 67 50,6 21 2.1 9 [9 1.16–3.1 11631 1056 10 6 105. 1592 15 159. 10000 00 100,0 506 50, 2. [ 1.16–3. 1163 1000 0 100, 1.16–3 116 1.16– 11 1.16 1.1 1.

RESUMO Objetivo: Este estudo teve como objetivo analisar a evolução espaçotemporal das taxas de incidência do vírus da imunodeficiência humana (HIV) e da síndrome da imunodeficiência adquirida (AIDS) no estado do Paraná, Brasil. Métodos: Foi realizado um estudo ecológico com componente analítico de séries temporais e análise espacial no estado do Paraná, de 2007 a 2022. A fonte de dados foi o Sistema de Informação de Agravos de Notificação. Para estudar a tendência, foi utilizado o modelo de regressão linear generalizada de Prais-Winsten por meio da decomposição de séries temporais e, para a análise espacial, foi aplicado o Índice de Moran. Resultados: A amostra total foi composta de 50.676 registros de HIV/AIDS. A taxa de incidência apresentou tendência crescente, com crescimento médio de 2,14% (intervalo de confiança de 95% — IC95% 1,16–3,13) ao mês. Nos períodos de 2007 a 2014 e 2015 a 2022, a média de casos no estado foi de 105,64 e 159,20 a cada 100 mil habitantes, respectivamente, com importantes variações entre os municípios. Agrupamentos espaciais de alto risco permaneceram na região metropolitana à capital e litoral e um novo agrupamento foi observado à região norte do estado. Conclusão: As taxas de incidência do HIV/AIDS apresentaram tendência temporal crescente. O número de casos variou substancialmente em alguns municípios, principalmente naqueles localizados na região litorânea. A análise espacial revelou padrões geoespaciais de alto risco nas principais regiões metropolitanas do Paraná: Curitiba (abrange o litoral), Londrina e Maringá, as quais compartilham características como elevado grau de urbanização e constante desenvolvimento econômico. Objetivo HIV (HIV AIDS (AIDS Paraná Brasil Métodos 200 2022 Notificação PraisWinsten Prais Winsten Moran Resultados 50676 50 676 50.67 HIVAIDS crescente 214 2 14 2,14 intervalo 95 IC95 IC 1,16–3,13 116313 1 16 3 13 mês 201 10564 105 64 105,6 15920 159 20 159,2 10 habitantes respectivamente municípios Conclusão litorânea abrange litoral, , litoral) Maringá econômico 202 5067 5 67 50.6 21 2,1 9 IC9 1,16–3,1 11631 1056 6 105, 1592 15 159, 506 50. 2, 1,16–3, 1163 1,16–3 116 1,16– 11 1,16 1,1 1,

Caracterizado por síntomas que permanecen o aparecen por primera vez dentro de los tres meses posteriores a la infección por SARS-CoV-2, la COVID larga puede manifestarse de diferentes formas, incluso entre casos no hospitalizados o asintomáticos. En este sentido, este artículo presenta un panorama la COVID larga en Brasil, con énfasis en el diagnóstico, los síntomas y los desafíos para la nueva gestión de la salud. Se utilizaron datos de una encuesta realizada para investigar la COVID larga en personas afectadas por COVID-19. Se trata de datos originales de una encuesta con individuos brasileños adultos (18 años o más), que tuvieron COVID-19, con datos recolectados entre el 14 de marzo y el 14 de abril de 2022, por medio de un cuestionario divulgado en las redes sociales. El cuestionario abordó características sociodemográficas, historial de infecciones por COVID-19, vacunación contra la enfermedad, investigación de la situación de salud y de la calidad de vida antes y después de COVID-19, además de la búsqueda y acceso a tratamiento. De los 1.728 encuestados, 720 fueron considerados elegibles para el análisis. De ellos, 496 (69%) tenían COVID larga. Las personas con COVID larga informaron manifestaciones clínicas como ansiedad (80%), pérdida de memoria (78%), dolor generalizado (77%), falta de atención (75%), fatiga (73%), pérdida de cabello (71%), cambios en el sueño (70%), cambios de humor (62%), malestar (60%) y dolor en las articulaciones (59%). La mayoría recurrió a los servicios de salud durante y después de la fase aguda de COVID-19 (94% y 80%, respectivamente), lo que representa la necesidad de estructurar el sistema de salud para atender a estos pacientes. SARSCoV2, SARSCoV2 SARSCoV SARS CoV 2, 2 SARS-CoV-2 formas asintomáticos sentido Brasil diagnóstico COVID19. COVID19 19. 19 18 (1 más, más , más) COVID19, 19, 1 2022 sociales sociodemográficas enfermedad tratamiento 1728 728 1.72 encuestados 72 análisis ellos 49 69% 69 (69% 80 80% (80%) 78%, 78 78% (78%) 77%, 77 77% (77%) 75%, 75 75% (75%) 73%, 73 73% (73%) 71%, 71 71% (71%) 70%, 70 70% (70%) 62%, 62 62% (62%) 60% 60 (60% 59%. 59 59% . (59%) COVID-1 94% 94 (94 respectivamente, respectivamente respectivamente) pacientes SARS-CoV- COVID1 ( 202 172 1.7 7 4 6 (69 8 (80% (78% (77% (75% (73% (71% (70% (62% (60 5 (59% COVID- 9 (9 SARS-CoV 20 17 1. (6 (80 (78 (77 (75 (73 (71 (70 (62 (59 (8 (7 (5

Characterized by symptoms that remain or appear for the first time within three months of SARS-CoV-2 infection, long COVID can manifest itself in different ways, including in non-hospitalized or asymptomatic cases. Thus, this study offers an overview of long COVID in Brazil, especially of its diagnosis, symptoms, and challenges for new health management. Data from a study that investigated long COVID in people affected by COVID-19 were used. These original data stem from a survey with adult Brazilians (aged 18 years or older) who had COVID-19 that collected information from March 14 to April 14, 2022, by a questionnaire on social media. The questionnaire addressed sociodemographic characteristics, history of COVID-19 infections, vaccination against the disease, investigation of health status and quality of life before and after COVID-19, and search and access to treatment. Of the 1,728 respondents, 720 were considered eligible for analysis, of which 496 (69%) had long COVID. Individuals with long COVID reported clinical manifestations such as anxiety (80%), memory loss (78%), generalized pain (77%), lack of attention (75%), fatigue (73%), hair loss (71%), sleep changes (70%), mood swings (62%), malaise (60%), and joint pain (59%). Most sought health services during and after the acute phase of COVID-19 (94 and 80%, respectively), representing the need to structure the healthcare system for these patients. SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- infection ways nonhospitalized non hospitalized cases Thus Brazil diagnosis management COVID19 19 COVID-1 used aged 1 older 2022 media characteristics infections disease COVID19, 19, treatment 1728 728 1,72 respondents 72 analysis 49 69% 69 (69% 80 80% , (80%) 78%, 78 78% (78%) 77%, 77 77% (77%) 75%, 75 75% (75%) 73%, 73 73% (73%) 71%, 71 71% (71%) 70%, 70 70% (70%) 62%, 62 62% (62%) 60%, 60 60% (60%) 59%. 59 59% . (59%) 94 (9 respectively, respectively respectively) patients SARS-CoV COVID1 COVID- 202 172 1,7 7 4 6 (69 8 (80% (78% (77% (75% (73% (71% (70% (62% (60% 5 (59% 9 ( 20 17 1, (6 (80 (78 (77 (75 (73 (71 (70 (62 (60 (59 (8 (7 (5

Caracterizada por sintomas que permanecem ou aparecem pela primeira vez em até três meses após a infecção pelo SARS-CoV-2, a COVID longa pode se manifestar de diferentes formas, inclusive entre casos não hospitalizados ou assintomáticos. Nesse sentido, este artigo apresenta um panorama da COVID longa no Brasil, com ênfase no diagnóstico, nos sintomas e nos desafios para a nova gestão da saúde. Foram utilizados dados de um estudo realizado com objetivo de investigar a COVID longa em pessoas acometidas pela COVID-19, com dados originais de um inquérito com indivíduos brasileiros adultos (18 anos ou mais) que tiveram COVID-19, coletados entre 14 de março e 14 de abril de 2022, por meio de questionário divulgado em redes sociais. O questionário abordou características sociodemográficas, histórico de infecções por COVID-19, vacinação contra a doença, investigação da situação de saúde e da qualidade de vida antes e após a COVID-19, além da busca e acesso a tratamento. Dos 1.728 respondentes, 720 foram considerados elegíveis para a análise. Desses, 496 (69%) tiveram COVID longa. Os indivíduos com COVID longa reportaram manifestações clínicas como ansiedade (80%), perda de memória (78%), dor generalizada (77%), falta de atenção (75%), fadiga (73%), queda de cabelo (71%), alterações de sono (70%), alterações de humor (62%), indisposição (60%) e dor nas articulações (59%). A maioria procurou os serviços de saúde durante e após a fase aguda de COVID-19 (94% e 80%, respectivamente), o que representa a necessidade de estruturar o sistema de saúde para atender esses pacientes. SARSCoV2, SARSCoV2 SARSCoV SARS CoV 2, 2 SARS-CoV-2 formas assintomáticos sentido Brasil diagnóstico COVID19, COVID19 19, 19 18 (1 mais 1 2022 sociais sociodemográficas doença tratamento 1728 728 1.72 respondentes 72 análise Desses 49 69% 69 (69% 80 80% , (80%) 78%, 78 78% (78%) 77%, 77 77% (77%) 75%, 75 75% (75%) 73%, 73 73% (73%) 71%, 71 71% (71%) 70%, 70 70% (70%) 62%, 62 62% (62%) 60% 60 (60% 59%. 59 59% . (59%) COVID-1 94% 94 (94 respectivamente, respectivamente respectivamente) pacientes SARS-CoV- COVID1 ( 202 172 1.7 7 4 6 (69 8 (80% (78% (77% (75% (73% (71% (70% (62% (60 5 (59% COVID- 9 (9 SARS-CoV 20 17 1. (6 (80 (78 (77 (75 (73 (71 (70 (62 (59 (8 (7 (5

Caracterizado por síntomas que permanecen o aparecen por primera vez dentro de los tres meses posteriores a la infección por SARS-CoV-2, la COVID larga puede manifestarse de diferentes formas, incluso entre casos no hospitalizados o asintomáticos. En este sentido, este artículo presenta un panorama la COVID larga en Brasil, con énfasis en el diagnóstico, los síntomas y los desafíos para la nueva gestión de la salud. Se utilizaron datos de una encuesta realizada para investigar la COVID larga en personas afectadas por COVID-19. Se trata de datos originales de una encuesta con individuos brasileños adultos (18 años o más), que tuvieron COVID-19, con datos recolectados entre el 14 de marzo y el 14 de abril de 2022, por medio de un cuestionario divulgado en las redes sociales. El cuestionario abordó características sociodemográficas, historial de infecciones por COVID-19, vacunación contra la enfermedad, investigación de la situación de salud y de la calidad de vida antes y después de COVID-19, además de la búsqueda y acceso a tratamiento. De los 1.728 encuestados, 720 fueron considerados elegibles para el análisis. De ellos, 496 (69%) tenían COVID larga. Las personas con COVID larga informaron manifestaciones clínicas como ansiedad (80%), pérdida de memoria (78%), dolor generalizado (77%), falta de atención (75%), fatiga (73%), pérdida de cabello (71%), cambios en el sueño (70%), cambios de humor (62%), malestar (60%) y dolor en las articulaciones (59%). La mayoría recurrió a los servicios de salud durante y después de la fase aguda de COVID-19 (94% y 80%, respectivamente), lo que representa la necesidad de estructurar el sistema de salud para atender a estos pacientes.

Caracterizada por sintomas que permanecem ou aparecem pela primeira vez em até três meses após a infecção pelo SARS-CoV-2, a COVID longa pode se manifestar de diferentes formas, inclusive entre casos não hospitalizados ou assintomáticos. Nesse sentido, este artigo apresenta um panorama da COVID longa no Brasil, com ênfase no diagnóstico, nos sintomas e nos desafios para a nova gestão da saúde. Foram utilizados dados de um estudo realizado com objetivo de investigar a COVID longa em pessoas acometidas pela COVID-19, com dados originais de um inquérito com indivíduos brasileiros adultos (18 anos ou mais) que tiveram COVID-19, coletados entre 14 de março e 14 de abril de 2022, por meio de questionário divulgado em redes sociais. O questionário abordou características sociodemográficas, histórico de infecções por COVID-19, vacinação contra a doença, investigação da situação de saúde e da qualidade de vida antes e após a COVID-19, além da busca e acesso a tratamento. Dos 1.728 respondentes, 720 foram considerados elegíveis para a análise. Desses, 496 (69%) tiveram COVID longa. Os indivíduos com COVID longa reportaram manifestações clínicas como ansiedade (80%), perda de memória (78%), dor generalizada (77%), falta de atenção (75%), fadiga (73%), queda de cabelo (71%), alterações de sono (70%), alterações de humor (62%), indisposição (60%) e dor nas articulações (59%). A maioria procurou os serviços de saúde durante e após a fase aguda de COVID-19 (94% e 80%, respectivamente), o que representa a necessidade de estruturar o sistema de saúde para atender esses pacientes.

Characterized by symptoms that remain or appear for the first time within three months of SARS-CoV-2 infection, long COVID can manifest itself in different ways, including in non-hospitalized or asymptomatic cases. Thus, this study offers an overview of long COVID in Brazil, especially of its diagnosis, symptoms, and challenges for new health management. Data from a study that investigated long COVID in people affected by COVID-19 were used. These original data stem from a survey with adult Brazilians (aged 18 years or older) who had COVID-19 that collected information from March 14 to April 14, 2022, by a questionnaire on social media. The questionnaire addressed sociodemographic characteristics, history of COVID-19 infections, vaccination against the disease, investigation of health status and quality of life before and after COVID-19, and search and access to treatment. Of the 1,728 respondents, 720 were considered eligible for analysis, of which 496 (69%) had long COVID. Individuals with long COVID reported clinical manifestations such as anxiety (80%), memory loss (78%), generalized pain (77%), lack of attention (75%), fatigue (73%), hair loss (71%), sleep changes (70%), mood swings (62%), malaise (60%), and joint pain (59%). Most sought health services during and after the acute phase of COVID-19 (94 and 80%, respectively), representing the need to structure the healthcare system for these patients.

The volatile composition of coffee exerts a substantial influence on its quality, as it defines the characteristics of the beverage. However, these compounds are influenced by factors within the coffee production chain, such as botanical origin, geography, processing methods, and roasting. Consequently, the identification of such compounds becomes a vital tool for characterizing coffees to these factors. In this context, gas chromatography with headspace extraction is widely used for aroma analysis, providing a composition closer to consumer perception. Headspace extraction offers speed, simplicity, minimal sample preparation, and no need for solvents. In this study, static headspace extraction (SHS) coupled with gas chromatography-mass spectrometry (SHS-GC-MS) was employed to establish the chemical profile of volatile compounds in Coffea arabica and Coffea canephora var. conilon and determine discriminants between the species. A total of 97 compounds, belonging to 17 chemical classes, were identified. The chemometric analysis highlighted furans, phenols, and carboxylic acids as key differentiating classes. Notably, furfuryl alcohol, acetic acid, 4-vinylguaiacol, N-acetyl-4(H)-pyridine, and N-furfurylpyrrole emerged as crucial volatile compounds. The variable selection using Fisher weight applied directly in the chromatograms, produced models consistent with relative area data, with furfuryl alcohol and 4-vinylguaiacol regions being particularly influential in differentiation. quality beverage However chain origin geography methods roasting Consequently context perception speed simplicity preparation solvents study SHS (SHS chromatographymass mass SHSGCMS GC MS (SHS-GC-MS var species 9 1 classes identified furans phenols Notably acid 4vinylguaiacol, 4vinylguaiacol vinylguaiacol 4 vinylguaiacol, Nacetyl4Hpyridine, Nacetyl4Hpyridine NacetylHpyridine N acetyl H pyridine, pyridine N-acetyl-4(H)-pyridine Nfurfurylpyrrole furfurylpyrrole chromatograms data differentiation Nacetyl Hpyridine

ABSTRACT Objective: The aim of this study was to analyze the spatiotemporal evolution of the incidence rates of human immunodeficiency virus (HIV) and acquired immune deficiency syndrome (AIDS) in the state of Paraná, Brazil. Methods: An ecological study with an analytical component of time series analysis was conducted in the state of Paraná from 2007 to 2022. The data source was the Notifiable Diseases Information System. To study the trend, the Prais–Winsten generalized linear regression model was used by decomposing the time series, and for spatial analysis, the Moran's index was applied. Results: The total sample consisted of 50,676 HIV/AIDS records. The incidence rate showed an increasing trend, with an average growth of 2.14% [95% confidence interval – 95%CI 1.16–3.13] per month. From 2007 to 2014 and from 2015 to 2022, the average number of cases in the state was 105.64 and 159.20 per 100,000 inhabitants, respectively, with significant variation among municipalities. Spatial clusters of high risk persisted in the metropolitan region, the capital, and coastal areas, and a new cluster was observed in the northern region of the state. Conclusion: The incidence rates of HIV/AIDS showed an upward trend over time. The number of cases varied considerably in some municipalities, especially in the coastal region. Spatial analysis revealed geospatial patterns of high risk in the main metropolitan areas of Paraná: Curitiba (including the coastal area), Londrina, and Maringá, which share characteristics such as a high degree of urbanization and ongoing economic development.

RESUMO Objetivo: Este estudo teve como objetivo analisar a evolução espaçotemporal das taxas de incidência do vírus da imunodeficiência humana (HIV) e da síndrome da imunodeficiência adquirida (AIDS) no estado do Paraná, Brasil. Métodos: Foi realizado um estudo ecológico com componente analítico de séries temporais e análise espacial no estado do Paraná, de 2007 a 2022. A fonte de dados foi o Sistema de Informação de Agravos de Notificação. Para estudar a tendência, foi utilizado o modelo de regressão linear generalizada de Prais-Winsten por meio da decomposição de séries temporais e, para a análise espacial, foi aplicado o Índice de Moran. Resultados: A amostra total foi composta de 50.676 registros de HIV/AIDS. A taxa de incidência apresentou tendência crescente, com crescimento médio de 2,14% (intervalo de confiança de 95% — IC95% 1,16–3,13) ao mês. Nos períodos de 2007 a 2014 e 2015 a 2022, a média de casos no estado foi de 105,64 e 159,20 a cada 100 mil habitantes, respectivamente, com importantes variações entre os municípios. Agrupamentos espaciais de alto risco permaneceram na região metropolitana à capital e litoral e um novo agrupamento foi observado à região norte do estado. Conclusão: As taxas de incidência do HIV/AIDS apresentaram tendência temporal crescente. O número de casos variou substancialmente em alguns municípios, principalmente naqueles localizados na região litorânea. A análise espacial revelou padrões geoespaciais de alto risco nas principais regiões metropolitanas do Paraná: Curitiba (abrange o litoral), Londrina e Maringá, as quais compartilham características como elevado grau de urbanização e constante desenvolvimento econômico.

Abstract Introduction: with the COVID-19 pandemic and the social distancing policy, almost 9 million workers had their professional activities transferred to the home environment, called “remote work,” “home office,” or “telework.” This context worked as a laboratory in which companies could experiment with teleworking. Objective: to analyze the unprecedented telework growth and the teleworkers' profile, highlighting the impacts and consequences for the workers' health. Methods: analysis of relevant bibliography, exploration of secondary data from empirical research on objective and subjective teleworking conditions, publications in the media about telework in the pandemic and post-pandemic context, and analysis of remote work ads. Results: due to the way remote work occurred during the pandemic, in addition to its consequences for health, and living and working conditions, new challenges were raised for the working class. Among them: how to ensure adequate environmental and ergonomic working conditions and how to guarantee the limit of working hours, and the delimitation of working time, in view of the tendency for telework to be maintained in the post-pandemic period. Conclusion: the analysis revealed impacts on workers’ health that bring new challenges to the working class. Such impacts, among other reasons, are due to extended working hours, ergonomic conditions at home, and pressure to meet goals. Introduction COVID19 COVID 19 COVID-1 policy environment work, office, office telework. “telework. Objective teleworkers profile Methods bibliography postpandemic post ads Results class them hours time period Conclusion reasons goals COVID1 1 COVID- “telework

Resumo Introdução: com a pandemia de COVID-19 e a política de distanciamento social, quase 9 milhões de trabalhadores tiveram suas atividades profissionais transferidas para o ambiente doméstico. Esse contexto funcionou como um laboratório no qual as empresas puderam experimentar a modalidade do teletrabalho. Objetivo: analisar o crescimento do teletrabalho e o perfil dos teletrabalhadores, destacando impactos e consequências para saúde dos profissionais. Métodos: análise de bibliografia pertinente, exploração de dados secundários de pesquisas sobre condições objetivas e subjetivas do teletrabalho, publicações sobre o teletrabalho no contexto da pandemia e pós-pandemia, análise de anúncios de trabalho remoto. Resultados: em razão da forma como ocorreu durante a pandemia, o trabalho remoto, além das consequências para saúde e condições de vida e trabalho, acarretou novos desafios para a classe trabalhadora. Entre eles, destacam-se: como assegurar condições ambientais e ergonômicas adequadas ao trabalho e como garantir o limite de jornada, a delimitação do tempo de trabalho, tendo em vista a tendência de sua manutenção para o período pós-pandemia. Conclusão: a análise revelou impactos sobre a saúde dos trabalhadores, com novos desafios para a classe trabalhadora. Tais impactos, entre outras razões, se devem a jornadas ampliadas, condições ergonômicas no domicílio e pressões para cumprimento de metas. Introdução COVID19 COVID 19 COVID-1 social doméstico Objetivo teletrabalhadores Métodos pertinente póspandemia, póspandemia pós pós-pandemia remoto Resultados trabalhadora eles destacamse destacam destacam-se jornada póspandemia. pandemia. Conclusão razões ampliadas metas COVID1 1 COVID-

The process of photosynthesis involves the absorption of light energy by photosynthetic pigments found in the thylakoid membrane of chloroplasts in order to produce chemical energy. In the presence of photosynthetic inhibitors, plant development is affected mainly by the reduction of the electron chain, which leads to growth inhibition. In this context, chlorophyll a (Chl a) fluorescence is an important technique used to identify the effects of inhibitors on the photosynthetic apparatus employing the JIP-test, which correlates the Chl a fluorescence transient to biophysical parameters, providing valuable information about the efficiency of photosystems I and II. Natural products have been highlighted as photosynthesis inhibitors due to the continued use of synthetic herbicides, which leads to the development of invasive plants resistant to these pesticides, in addition to the toxicity caused to humans and the environment. Thus, this review describes the main applications of extracts and isolated secondary metabolites obtained from plants and microorganisms in the investigation of electron transport inhibition on photosystem II. chain context JIPtest, JIPtest JIP test, test JIP-test parameters II herbicides pesticides environment Thus

Abstract Background: Systemic inflammatory responses mimicking infectious complications are often present in surgical patients. Methods: The objective was to assess the association between withholding early antimicrobial therapy while investigating alternative diagnoses and worse outcomes in nonseptic patients with suspected nosocomial infection in a retrospective cohort of critically ill surgical patients. The initiation of antibiotic therapy within 24 h of the suspicion of infection was defined as the Early Empirical Antibiotic strategy (EEA) group and the initiation after 24 h of suspicion or not prescribed was defined as the Conservative Antibiotic strategy (CA) group. Primary outcome was composite: death, sepsis, or septic shock within 14 days. Main exclusion criteria were sepsis or an evident source of infection at inclusion. Results: Three hundred and forty patients were eligible for inclusion (74% trauma patients). Age, sex, reason for hospital admission, SAPS3 score, SOFA score, and use of vasopressors or mechanical ventilation were not different between the groups. Within 14 days of inclusion, 100% (130/130) of EEA patients received antibiotics compared to 57% (120/210) of CA patients. After adjusting for confounding variables, there was no association between primary outcome and the groups. In a post hoc subgroup analysis including only patients with a posteriori confirmed infection (by microbiological cultures), delay in initiation of adequate antimicrobial therapy was independently associated with the primary outcome (Odds Ratio = 1.19 per day of delay; 95% CI 1.05–1.37). Conclusions: Withholding early empiric antibiotic therapy was not associated with progression of organ dysfunction within 14 days in nonseptic surgical patients with suspected nosocomial infection without an obvious source. Background Methods 2 (EEA (CA composite death 1 Results 74% 74 (74 . patients) Age sex admission SAPS score groups 100 130/130 130130 130 (130/130 57 120/210 120210 120 210 (120/210 variables by cultures, cultures , cultures) Odds 119 19 1.1 95 1.05–1.37. 105137 1.05–1.37 05 37 1.05–1.37) Conclusions 7 (7 10 130/13 13013 13 (130/13 5 120/21 12021 12 21 (120/21 11 1. 9 10513 1.05–1.3 0 3 ( 130/1 1301 (130/1 120/2 1202 (120/2 1051 1.05–1. 130/ (130/ 120/ (120/ 105 1.05–1 (130 (120 1.05– (13 (12 1.05 (1 1.0