Abstract A lectin from the marine sponge Haliclona (Reniera) implexiformis (HiL) was isolated by affinity chromatography on Sepharose™ matrix. HiL showed specificity for galactose and its derivatives. The glycoproteins porcine stomach mucin (PSM) and bovine stomach mucin (BSM) were potent inhibitors. Hemagglutinating activity of the lectin was maximal between pH 5.0 and 9.0. The lectin remained active until 60°C. The presence of CaCl2 and EDTA did not affect the hemagglutinating activity. In SDS-PAGE, HiL showed a single band of 20 kDa under reduced conditions, whereas in the non-reducing conditions, it showed a band of 20 kDa and one additional band of 36 kDa. The average molecular mass determined by Electrospray Ionization Mass Spectrometry (ESI-MS) was 35.874 ± 2 Da in native and non-reducing conditions, whereas carboxyamidomethylated-lectin showed 18,111 Da. These data indicated that HiL consists in a dimer formed by identical subunits linked by disulfide bonds. Partial amino acid sequence of HiL was determined by mass spectrometry, and revealed that it is a new type of lectin, which showed no similarity with any protein. Secondary structure consisted of 6% α-helice, 31% β-sheet, 18% β-turn and 45% random coil. HiL showed significant reduction in the number of viable cells of Staphylococcus biofilms. Reniera (Reniera (HiL Sepharose matrix derivatives PSM (PSM BSM (BSM inhibitors 50 5 0 5. 90 9 9.0 60C C 60 60°C CaCl SDSPAGE, SDSPAGE SDS PAGE, PAGE SDS-PAGE conditions nonreducing non reducing 3 ESIMS ESI MS (ESI-MS 35874 35 874 35.87 carboxyamidomethylatedlectin carboxyamidomethylated 18111 18 111 18,11 bonds spectrometry protein 6 αhelice, αhelice α helice, helice α-helice 31 βsheet, βsheet β sheet, sheet β-sheet βturn turn 45 coil biofilms 9. 3587 87 35.8 1811 1 11 18,1 4 358 8 35. 181 18,

Resumen El artículo presenta la metodología y los resultados de un estudio que propone un conjunto de indicadores locales para monitorear la evolución de la Agenda de Desarrollo Sostenible de las Naciones Unidas 2030 en comunidades caiçaras y quilombolas en el territorio de Bocaina (municipios de Paraty, Rio de Janeiro y Ubatuba, S.Paulo). El objetivo fue adaptar una agenda global/nacional al nivel local y elaborar indicadores específicos capaces de aprehender su “localización”. Inicialmente se seleccionaron 40 metas de la Agenda 2030, adaptadas a las condiciones del territorio; luego, una investigación cualitativa con las comunidades identificó problemáticas locales para lograr un desarrollo sostenible y las alternativas desarrolladas para abordarlas. A partir de estos y de datos secundarios con información socioeconómica, ambiental y de prestación de servicios públicos para estas poblaciones, se elaboraron 87 indicadores locales, para monitorear las metas y objetivos de la Agenda 2030 en el território. 203 municipios Paraty Ubatuba S.Paulo. SPaulo S.Paulo . S Paulo S.Paulo) globalnacional global nacional localización. localización “localización” 4 luego abordarlas socioeconómica poblaciones 8 território 20 “localización 2

Abstract This article presents the methodology and findings of a study that proposed a set of local indicators for monitoring progress on the United Nations Sustainable Development Agenda 2030 in caiçara and quilombola communities in the territory of Bocaina in the municipalities of Paraty (Rio de Janeiro) and Ubatuba (São Paulo). The objective was to adapt a global and Brazilian national agenda to the territory level and to construct specific indicators to reflect that “localisation”. To begin with, 40 goals of Agenda 2030 were selected and adapted to conditions in the territory. Then a qualitative study of quilombolas and caiçaras identified local problems in achieving sustainable development and the alternative solutions developed to address those problems. On the basis of that study and secondary data from socioeconomic, environmental and public service information on those populations, 87 local indicators were constructed for the purpose of monitoring progress towards Agenda 2030 targets and goals in the territory. 203 Rio Janeiro São Paulo. Paulo . Paulo) localisation. localisation “localisation” with 4 socioeconomic populations 8 20 “localisation 2

Resumo O artigo apresenta a metodologia e os resultados de um estudo que propõe um conjunto de indicadores locais para acompanhar a evolução da Agenda 2030 do Desenvolvimento Sustentável da Organização das Nações Unidas em comunidades caiçaras e quilombolas no território da Bocaina, nos municípios de Paraty (Rio de Janeiro) e Ubatuba (S.Paulo). O objetivo foi adaptar uma agenda global/nacional ao nível do território e construir indicadores específicos capazes de apreender sua “localização”. Inicialmente foram selecionadas 40 metas da Agenda 2030 adaptadas às condições do território; em seguida, uma pesquisa qualitativa com quilombolas e caiçaras identificou problemas locais para alcançar o desenvolvimento sustentável e alternativas desenvolvidas para enfrentá-los. Com base nessa pesquisa e em dados secundários com informações socioeconômicas, ambientais e sobre oferta de serviços públicos para essas populações, foram construídos 87 indicadores locais com o objetivo de acompanhar as metas e objetivos da Agenda 2030 no território. 203 Bocaina Rio Janeiro S.Paulo. SPaulo S.Paulo . S Paulo (S.Paulo) globalnacional global nacional localização. localização “localização” 4 seguida enfrentálos. enfrentálos enfrentá los. los enfrentá-los socioeconômicas populações 8 20 (S.Paulo “localização 2

Abstract A new lectin from marine sponge Ircinia strobilina, denominated IsL, was isolated by combination of affinity chromatography in Guar gum matrix followed by size exclusion chromatography. IsL was able to agglutinate native and enzymatically treated rabbit erythrocytes, being inhibited by galactosides, such as α-methyl-D-galactopyranoside, β-methyl-D-galactopyranoside and α-lactose. IsL hemagglutinating activity was stable at neutral to alkaline pH, however the lectin loses its activity at 40° C. The molecular mass determinated by mass spectrometry was 13.655 ± 5 Da. Approximately 40% of the primary structure of IsL was determined by mass spectrometry, but no similarity was observed with any protein. The secondary structure of IsL consists of 28% α-helix, 26% β-sheet, and 46% random region, as determined by dichroism circular. IsL was a calcium-dependent lectin, but no significant variations were observed by circular dichroism when IsL was incubated in presence of calcium and EDTA. IsL was not toxic against Artemia nauplii and did not have antimicrobial activity against bacterial cells. However, the IsL was able to significantly inhibit the biofilm formation of Staphylococcus aureus and Staphylococcus epidermidis. strobilina erythrocytes galactosides αmethylDgalactopyranoside, αmethylDgalactopyranoside α methyl D galactopyranoside, galactopyranoside α-methyl-D-galactopyranoside βmethylDgalactopyranoside β αlactose. αlactose lactose. lactose α-lactose pH 40 C 13655 13 655 13.65 Da protein 28 αhelix, αhelix helix, helix α-helix 26 βsheet, βsheet sheet, sheet β-sheet 46 region calciumdependent dependent EDTA cells However epidermidis 4 1365 1 65 13.6 2 136 6 13.

ABSTRACT Purpose: To evaluate the erectile function in patients who underwent partial penectomy and identify factors associated with penile functional status. Materials and Methods: We identified patients who underwent partial penectomy due to penile cancer between 2009 and 2014. Clinical and pathological characteristics included patient age at the time of diagnosis, obesity, hypertension, dyslipidemia, diabetes, smoking, metabolic syndrome, Eastern Cooperative Oncology Group (ECOG) status, penile shaft length, tumor size, primary tumor stage (pT), clinical nodal status, and local recurrence. Erectile function was assessed prospectively with the International Index of Erectile Function (IIEF-5) at least 3 months after partial penectomy. Results: A total of 81 patients met analysis criteria. At the diagnosis, the median age was 62 years (range from 30 to 88). Median follow-up was 17 months (IQR 7-36). Of total patients, 37 (45%) had T2 or higher disease. Clinically positive nodes were present in 16 (20%) patients and seven (8.6%) developed local recurrence. Fifty patients (62%) had erectile dysfunction (ED) after partial penectomy, 30% had moderate or severe erectile dysfunction scores. Patients with ED versus without ED were similar in baseline characteristics except for age, penile shaft length, and presence of inguinal adenopathy (p <0.05). Multivariate analysis using logistic regression confirmed that older patients, shorter penile shaft length, and clinically positive lymph node were significantly associated with ED. Conclusion: Partial penectomy due to penile cancer provides adequate local control of the disease, however, proper counselling is important especially in relation to ED consequences. Preservation of penile length yields to more optimal erectile recovery.

Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.

Resumo Maturação interfere em aspectos fisiológicos e mecânicos da contração muscular. O objetivo do estudo foi comparar a eficiência do ciclo alongamento-encurtamento (SSC) em diferentes estágios maturacionais. Cento e quarenta e seis jovens, sendo 72 meninos e 74 meninas, foram recrutados para participar do estudo. Maturação sexual foi mensurada e o grupo foi estratificado em adolescentes (≥ 3 e crianças (< estágio 2). Salto estático (SJ) e o salto contramovimento (CMJ) foram utilizados para mensurar a força reativa (RF), razão de utilização da excêntrica (EUR) e aumento do pré-estiramento (PSAP). As crianças não apresentaram diferença significante entre SJ e CMJ (t(55)= -1.93; ∆ = -0.61; 95% CI = -1.24 to -0.02; p = 0.058), Enquanto que os adolescentes apresentaram maior desempenho de CMJ (t(89)= -12.28; ∆ = -2.44; 95% CI = -2.84 to -2.05; p <0.001). Em adição, adolescentes apresentaram maior RF (t(144)= 5.18; p <0.0001; 95% CI = 2.53 to 1.13), PSAP (t(144)= 4.38; p <0.0001; 95% CI = 14.00 to 5.29) e EUR (t(144)= 4.38; p <0.0001; 95% CI = 0.14 to 0.05). Nós concluímos que jovens com mais maturados apresentam maior capacidade de utilização do SSC.

Abstract Maturation interferes with physiological and mechanical aspects of muscle contraction. The aim of the present study was to compare the efficiency of the stretch-shortening cycle (SSC) between different maturation stages. One hundred forty six young people, with 72 boys and 74 girls, were recruited to participate in the study. Sexual maturation was measured and the group was stratified into adolescents (> 3 auto-tanner) and children (<2 auto-tanner). Squat jump (SJ) and countermovement jump (CMJ) performance were measured to measure reactive force (RF) capacity, eccentric utilization ratio (EUR) and pre-stretch augmentation (PSAP). The children did not present statistical difference between SJ and CMJ (t(55)= -1.93; ∆ = -0.61; 95% CI = -1.24 to -0.02; p = 0.058), while the adolescents presented higher performance of CMJ (t(89)= -12.28; ∆ = -2.44; 95% CI = -2.84 to -2.05; p <0.001). In addition, adolescents had higher RF performance (t(144)= 5.18; p <0.0001; 95% CI = 2.53 to 1.13), PSAP (t(144)= 4.38; p <0.0001; 95% CI = 14.00 to 5.29) and EUR (t(144)= 4.38; p <0.0001; 95% CI = 0.14 to 0.05). We conclude that more mature young people have better SSC utilization.

Abstract This study aimed to analyze the influence of different maturational stages on performance-related physical fitness components, specifically upper limb explosive strength and jump height. A total of 239 children participated in the study, all of which were volleyball practitioners: 139 were female and 100 were male, aged 10 to 14 years old. Anthropometric assessments and physical capacity tests were performed. For the statistical analysis, Z-score of kurtosis and asymmetry for normality test, and Kruskall-Wallis test, Spearman's correlation and linear regression analysis were used. The girls presented superior results in bone age when compared to boys. Correlations between chronological age and bone age showed similar results. It was concluded that bone age shows a positive correlation with the explosive strength of upper limbs in both sexes.

Resumo O presente estudo teve como objetivo analisar a influência dos diferentes estágios maturacionais nos componentes da aptidão física relacionados ao desempenho, especificamente a força explosiva de membros superiores e a altura do salto. Participaram do estudo 239 crianças, sendo 139 do sexo feminino e 100 do sexo masculino com idades entre 10 e 14 anos, praticantes de voleibol. Foram realizadas avaliações antropométricas e testes de capacidades físicas. Na análise estatística foi utilizada o teste de normalidade, Z-score da curtose e assimetria, houve aplicação do teste de Kruskall-Wallis, correlação de Spearman e análise de regressão linear. As meninas apresentaram resultados superiores na idade óssea quando comparadas aos meninos. As correlações entre a idade cronológica e a idade óssea demonstraram resultados similares. Concluiu-se que a idade óssea retrata uma correlação positiva com a força explosiva de membros superiores em ambos os sexos.

O objetivo do presente estudo foi analisar o nível de flexibilidade em adolescentes praticantes de treinamento resistido através do Teste Sentar e Alcançar (TSA). Participaram do estudo 30 adolescentes de ambos os sexos que praticam treinamento de força em uma academia sediada na cidade de Fortaleza, no Estado do Ceará. Os resultados apresentados na classificação do teste, teve como destaque o resultado abaixo da média em 14(46,7%) dos avaliados. Pode-se concluir que, se torna necessário um melhor acompanhamento desses jovens para que tal capacidade física seja aprimorada.

The objective of the present study was to analyze the level of flexibility in adolescents practicing resistance training through the Sit and Go Test (TSA). Participated in the study thirty adolescents of both sexes who practice strength training at an academy based in the city of Fortaleza, in the State of Ceará. The results presented in the test classification had the result below average in 14 (46.7%) of the evaluated adolescents. It can be concluded that, it is necessary to better follow these young people so that this physical capacity is improved.

O objetivo do presente estudo foi investigar os principais motivos que levam idosos a buscar a hidroginástica como prática de atividade física. Foram investigados 60 indivíduos idosos de ambos os sexos que praticantes de hidroginástica. Pôde-se observar uma discrepância com relação ao gênero dos participantes, onde 86,7% do público era formado por mulheres e apenas 13,3% por homens. Pôde-se concluir que, a indicação médica foi a principal razão que levam os idosos a buscarem a prática da hidroginástica (56,7%).

The objective of the present study was to investigate the main reasons that lead elders to seek hydrogymnastics as a practice of physical activity. We investigated 60 elderly individuals of both sexes who practice water aerobics. There was a discrepancy with respect to the gender of the participants, where 86.7% of the audience was women and only 13.3% were men. It could be concluded that the medical indication was the main reason that lead the elderly to seek the practice of water aerobics (56.7%).

O objetivo do presente estudo foi avaliar a relação entre flexibilidade e a força das articulações do quadril, joelho e tornozelo de praticantes do CrossFit®. Foram investigados 16 indivíduos de ambos os sexos que praticam CrossFit® em dois boxes sediados na cidade de Fortaleza, no Estado do Ceará. Pôde-se observar que 18,75% da amostra total teve classificação referente a flexibilidade apresentada como pequena. A pesquisa verificou e identificou que, uma boa flexibilidade influencia no desempenho da força máxima nos praticantes de CrossFit® do grupo amostral.

The aim of the present study was to evaluate the relationship between flexibility and strength of the hip, knee and ankle joints of CrossFit® practitioners. We investigated 16 individuals of both sexes who practice CrossFit® in two boxes located in the city of Fortaleza, in the State of Ceará. It was observed that 18.75% of the total sample had a classification referring to the flexibility presented as small. The research verified and identified that, good flexibility influences the performance of maximum strength in CrossFit® practitioners of the sample group.

A presente revisão possui como objetivo identificar qual (is) o (s) tipo (s) de treinamento prescrito como forma de prevenção e reabilitação das lesões de Ligamento Cruzado Anterior (LCA) em jogadores de futebol. Para a realização deste trabalho foi realizada uma breve revisão da literatura acerca da prevenção e reabilitação das lesões de LCA em futebolistas. Foram avaliados artigos científicos, monografias, dissertações de mestrado, bem como livros, que tratassem da temática da lesão de LCA, mais concretamente no futebol. Verificou-se que existem possibilidades de prevenção da lesão positivas a serem utilizadas por profissionais da área da saúde (fisioterapeutas e profissionais de educação física) destacando-se os treinamentos neuromusculares e proprioceptivos. Foi identificado que, o treinamento a ser prescrito como forma de prevenção e reabilitação para lesões de ligamento cruzado anterior em jogadores de futebol são os neuromusculares e os proprioceptivos.

The purpose of this review is to identify the type (s) of training prescribed as a means of preventing and rehabilitating ACL injuries in soccer players. A brief review of the literature on the prevention and rehabilitation of ACL injuries in footballers was carried out to perform this work. Scientific articles, monographs, master's dissertations, as well as books dealing with the theme of ACL injury, more specifically in soccer, were evaluated. It was verified that there are positive possibilities of injury prevention to be used by health professionals (physiotherapists and physical education professionals), emphasizing neuromuscular and proprioceptive training. It has been identified that the training to be prescribed as a form of prevention and rehabilitation for anterior cruciate ligament injuries in soccer players is neuromuscular and proprioceptive.

RESUMO Objetivou-se quantificar a produção e a composição química do leite das ovelhas Rabo Largo mantidas sob dois sistemas de alimentação durante a fase lactacional. Foram utilizadas 60 ovelhas, primíparas e multíparas entre um e quatro anos de idade, amamentando um ou dois cordeiros, mantidas sob dois sistemas de alimentação, com peso vivo médio inicial de 43 kg e 40,5 kg para o lote suplementado e lote não suplementado, respectivamente. Foi medida a produção de leite semanalmente, através do método indireto da dupla pesagem, após o período do colostro. Foram coletadas também amostras do leite, a cada semana, para a determinação da composição química. Os dados de produção de leite foram analisados como medidas repetidas no tempo e entre grupo de fatores. Os fatores considerados foram ano e períodos chuvoso e seco no início de lactação, ordem de lactação, interação tipo de parto e sexo das crias e entre os lotes. Para efeito de comparação de médias entre os lotes, utilizou-se o teste de média dos mínimos quadrados com nível de significância de 5%. Verificou-se que o lote suplementado obteve maior produção de leite do que o lote não suplementado, exceto nas duas últimas semanas de lactação. O pico de produção ocorreu durante a segunda semana de lactação para ambos os lotes. As ovelhas da raça Rabo Largo sem acesso a suplementação responderam de maneira positiva expressando adaptabilidade as condições semiáridas com potencial leiteiro semelhante a outras raças ovinas, e quando suplementadas obtém-se maior produção e manutenção dos mais elevados níveis dos componentes químicos do leite na última semana de lactação.

SUMMARY The aim of this study was to quantify the production and the chemical composition of the milk of ewes of Rabo Largo breed raised under two feeding systems during the lactation. Sixty ewes, primiparous and multiparous, aged one to four, years nursing one or two lambs were raised under two feeding systems, with average body weight of 43 kg and 40.5 kg for the groups supplemented and not supplemented, respectively. The milk yield were measured weekly, using the indirect method of double weighing, after a period of colostrum. Milk samples were also collected every week for determination of the chemical composition. The data of daily milk production were analyzed with repeated measures over time and across groups of factors. The subject factors considered were year and rainy and dry periods in early lactation, lactation order, interaction between type of lambing and sex of the offspring and between the batches. For comparison of means between batches, we used the average of the least squares with a significance level of 5%. It was found that the supplemented group presented a higher milk yield than the not supplemented group, except to the two last weeks old lactation. Already peak production occurred during the second week of lactation for both lots. The dietary supplementation increases the production and maintained the highest levels of the chemical components of milk in the last week of lactation.

Resumo O estudo tem por objetivo investigar a relação entre os indicadores de governança do Banco Mundial e os indicadores de desenvolvimento socioeconômico nos países que formam o Brics. Foram utilizadas bases de dados de organizações internacionais do período de 2005 a 2012, aplicando-se a análise de dados em painel. Os resultados indicam relações positivas entre o IDH e os indicadores Eficácia do Governo e Controle da Corrupção e entre o PIB e o indicador Controle da Corrupção, além de uma relação negativa entre o PIB e os indicadores Voz e Responsabilização e Estabilidade Política. A capacidade explicativa das dimensões dos indicadores de governança em relação ao IDH é maior quando comparada à das dimensões dos indicadores de governança relacionadas com o PIB, o que pode ser entendido pelo fato de que o segundo indicador trata apenas da dimensão econômica, enquanto o IDH engloba outros fatores.

Abstract The purpose of this study is to investigate the relationship between the World Bank's governance indicators and the socioeconomic development indicators of the Brics countries. The databases of a number of international organizations corresponding to the 2005-12 period were assessed using panel data analysis. The results suggested a positive relationship between the HDI and the Government Effectiveness and Control of Corruption indicators, and between GDP and the Control of Corruption indicator, as well as a negative relationship between GDP and the Voice and Accountability and the Political Stability indicators. The explanatory power of the governance indicator dimensions in relation to the HDI is greater when compared to that of the dimensions of governance indicators related to GDP, which can be understood by the fact that the latter index only deals with the economic dimension whereas the HDI takes other factors into account.

Resumen El estudio tiene como objetivo investigar la relación entre los indicadores mundiales de gobernabilidad del Banco Mundial y los indicadores de desarrollo socioeconómico en los países del Brics. Se utilizaron bases de datos de organizaciones internacionales del período de 2005 a 2012, aplicándose el análisis de datos de panel. Los resultados indican relaciones positivas entre el IDH y los indicadores Efectividad del gobierno y Control de la corrupción y entre el PIB y el Control de la corrupción, además de una relación negativa entre el PIB y los indicadores Participación y monitoreo y Efectividad del gobierno. La capacidad explicativa de las dimensiones de los indicadores de gobernanza en relación al IDH es mayor cuando se compara con las dimensiones de los indicadores de gobernanza relacionados con el PIB, lo que puede ser entendido por el hecho de que el PIB trata apenas de la dimensión económica, mientras que el IDH engloba otros factores.

OBJETIVO: Avaliar a evolução do resultado funcional da hemiartroplastia CTA(r)no tratamento cirúrgico da artropatia degenerativa do manguito rotador com um seguimento médio de 5,4 anos.MÉTODOS: Foram reavaliados 18 pacientes submetidos à artroplastia parcial CTA(r) para o tratamento da artropatia degenerativa do manguito rotador entre abril de 2007 e junho de 2009, com seguimento mínimo e médio de 4,6 anos e 5,4 anos, respectivamente. Foram usados parâmetros pré e pós-operatórios de funcionalidade e satisfação dos pacientes (escala funcional da Universidade da Califórnia em Los Angeles [UCLA]). Todos os pacientes fizeram tratamento conservador prévio por seis meses e foram submetidos ao tratamento cirúrgico na ausência de resultado satisfatório. Foram excluídos pacientes com cirurgia prévia no ombro, pseudoparalisia, insuficiência do arco coracoacromial (tipo 2 B da classificação de Seebauer), lesão neurológica ou insuficiência do músculo deltoide e do músculo subescapular.RESULTADOS: Com um seguimento médio de 5,4 anos, 14 pacientes se consideravam satisfeitos com a cirurgia (78%). A amplitude de movimento articular melhorou na elevação ativa média e variou de 55,8° no pré-operatório para 82° no pós-operatório. A rotação externa média melhorou de em média 18,9° no pré-operatório para 27,3° no pós-operatório. A média da rotação medial manteve-se no nível da terceira vértebra lombar. O escore UCLA médio, após seguimento médio de 5,4 anos, foi de 23,94 e melhorou em comparação com as médias pré-operatória e do primeiro ano pós-operatório.CONCLUSÃO: Os resultados funcionais da hemiartroplastia CTA(r) no tratamento da artropatia do manguito rotador em pacientes selecionados mantiveram-se satisfatórios após um seguimento médio de 5,4 anos.

OBJECTIVE: To evaluate the evolution of the functional results from CTA(r)hemiarthroplasty for surgically treating degenerative arthroplathy of the rotator cuff, with a mean follow-up of 5.4 years.METHODS: Eighteen patients who underwent CTA(r) partial arthroplasty to treat degenerative arthroplathy of the rotator cuff between April 2007 and June 2009 were reevaluated, with minimum and mean follow-ups of 4.6 years and 5.4 years, respectively. Pre and postoperative parameters for functionality and patient satisfaction were used (functional scale of the University of California in Los Angeles, UCLA). All the patients underwent prior conservative treatment for 6 months and underwent surgical treatment because of the absence of satisfactory results. Patients were excluded if they presented any of the following: previous shoulder surgery; pseudoparalysis; insufficiency of the coracoacromial arch (type 2 B in Seebauer's classification); neurological lesions; or insufficiency of the deltoid muscle and the subscapularis muscle.RESULTS: With a mean follow-up of 5.4 years, 14 patients considered that they were satisfied with the surgery (78%); the mean range of joint motion for active elevation improved from 55.8° before the operation to 82.0° after the operation; the mean external rotation improved from 18.9° before the operation to 27.3° after the operation; and the mean medial rotation remained at the level of the third lumbar vertebra. The mean UCLA score after the mean follow-up of 5.4 years was 23.94 and this was an improvement in comparison with the preoperative mean and the mean 1 year after the operation.CONCLUSION: The functional results from CTA(r) hemiarthroplasty for treating rotator cuff arthroplasty in selected patients remained satisfactory after a mean follow-up of 5.4 years.

This research evaluated the fungistatic and fungicidal activities of red propolis alcoholic extract (RPAE) against different Candida species isolated from chronic periodontitis cases, and compared with chlorhexidine (CHX). Nineteen samples of Candida species (C. albicans [n = 12], C. tropicalis [n = 5] andC. glabrata[n = 2]) isolated from chronic periodontitis cases were analyzed. The fungistatic and fungicidal activity of both RPAE and CHX were evaluated using fluconazole and C. parapsilosis (ATCC 6258) as a control. Fungistatic activity was analyzed based on the Clinical and Laboratory Standards Institute (CLSI) reference procedure to determine the minimum inhibitory concentrations. Fungicidal activity was established according to the absence of fungal growth on Sabouraud Dextrose Agar medium. The fungistatic and fungicidal activities of RPAE were observed, respectively, at 32-64 μg/mL and 64-512 μg/mL for C.albicans, 64 μg/mL and 64-256 μg/mL for C. glabrata, and 32-64 μg/mL and 64 µg/mL for C. tropicalis. CHX fungistatic activity was observed at concentrations of 0.003-1.92 µg/mL for C. albicans, 1.92 µg/mL for C. glabrata, and 0.03-1.92 µg/mL for C. tropicalis. Fluconazole fungistatic activity ranged between 1-64 μg/mL, and fungicidal activity occurred at 8-64 μg/mL, for the threeCandida species analyzed. All the Candidaspecies were susceptible to RPAE antifungal activity, but five samples ofC.albicans, one ofC.tropicalis and one ofC.glabrata were resistant to fluconazole antifungal activity. CHX showed fungistatic activity against all the Candida species analyzed. The antifungal potential of these substances suggests that they can be applied as an alternative treatment for diseases affected by these species.