Pesquisa | SciELO

Abstract Background Hidradenitis suppurativa (HS) is a chronic inflammatory disease that leads to the formation of nodules, abscesses and fistulas, with the formation of scars and fibrosis, causing significant impairment in patient quality of life. The diagnosis is clinical, using scores to classify the severity of the condition; currently the most recommended classification is the International Hidradenitis Suppurativa Severity Scoring System (IHS4). Doppler ultrasound has been used to complement the clinical evaluation of patients with HS. It is possible to observe subclinical lesions that change the staging, the severity of the case, and its treatment, either clinical or surgical. Correct treatment is essential to minimize the consequences of this disease for the patient. Objective To establish an outpatient protocol for the use of Doppler ultrasound in the care of patients with HS. Methods A narrative review of the literature was carried out on the use of Doppler ultrasound in patients with hidradenitis suppurativa; a referring protocol and technique orientations for imaging assessment in HS were created. Results Recommendation to perform ultrasound evaluation of symptomatic areas eight weeks after using antibiotics and four, 12, and 24 weeks after starting immunobiologicals; apply SOS-HS ultrasound severity classification. Study limitations The review did not cover all literature on ultrasound and HS; no systematic review was carried out, but rather a narrative one. Conclusions The correct assessment of patients staging must be carried out using dermatological ultrasound to avoid progression to scars and fibrosis, which compromise patients quality of life. (HS nodules fistulas fibrosis life condition IHS4. IHS4 IHS . (IHS4) case surgical created four 12 2 immunobiologicals SOSHS SOS one (IHS4 1 (IHS

https://doi.org/10.1016/j.abd.2023.10.003

Prevalência de Sintomas Miccionais e Fatores Preditivos em Transplantados Renais em um Centro Transplantador Brasileiro
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Gomes Sobrinho, Diego Henrique

; Tomaz, Kézia Jahel Santos

; Schwamback, Guilherme Rodrigues

; Rodrigues, Renata Gonçalves Santos

; Santos, Alessandro Corrêa Prudente dos

Brazilian Journal of Transplantation

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2024, Volume 27 elocation e1024

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Objectives: Identify relationships between voiding symptom scores and variables related to transplantation. Methods: Observational, cross-sectional, and analytical study, in which all patients undergoing kidney transplantation in the state of Rondônia, Brazil, were evaluated through interviews and analysis of records, regarding their clinical-demographic profile and signs and symptoms of voiding dysfunction. Chi-square, analysis of variance (ANOVA), and multivariate linear regression were used to establish possible correlations. Results: Eight-one point eighty-seven percent of patients were approached (n = 122). The most common symptoms were increased frequency (68.2%) and nocturia (97.6%). ANOVA demonstrated a relationship between age and International Consultation on Incontinence Questionnaire (ICIQ) (p = 0.014), alcoholism and Overactive Bladder Questionnaire (OAB) (p = 0.001), immunosuppressive regimen and ICIQ (p = 0.04), thymoglobulin and OAB (p = 0.009), use of double J and ICIQ (p = 0.014), citomegalovirus infection and OAB (p = 0.031), and International Prostate Symptom Score (IPSS) (p = 0.008). Of the comorbidities, it was observed heart failure related to OAB (p = 0.009) and ICIQ (p = 0.003) scores. Chi-square showed an association between alcohol use and group 1 of the OAB (p = 0.005). The Pearson test positively correlated graft ischemia time with ICIQ (p = 0.04). Conclusion: The prevalence of lower urinary tract symptoms is higher in kidney transplant recipients than in the general population, and it was possible to determine predictive factors for their occurrence. Objectives Methods Observational crosssectional, crosssectional cross sectional, sectional cross-sectional study Rondônia Brazil records clinicaldemographic clinical demographic dysfunction Chisquare, Chisquare Chi square, square ANOVA, , (ANOVA) correlations Results Eightone Eight one eightyseven eighty seven n 122. 122 . 122) 68.2% 682 68 2 (68.2% 97.6%. 976 97.6% 97 6 (97.6%) (ICIQ p 0.014, 0014 0.014 0 014 0.014) (OAB 0.001, 0001 0.001 001 0.001) 0.04, 004 0.04 04 0.04) 0.009, 0009 0.009 009 0.031, 0031 0.031 031 0.031) IPSS (IPSS 0.008. 0008 0.008 008 0.008) comorbidities 0.003 0003 003 0.005. 0005 0.005 005 0.005) 0.04. Conclusion population occurrence (ANOVA 12 68.2 (68.2 97.6 9 (97.6% 0.01 01 000 0.00 00 0.0 0.03 03 68. (68. 97. (97.6 0. (68 (97. (6 (97 ( (9

RESUMO Objetivos: Identificar relações entre escores de sintomas miccionais e variáveis relacionadas ao transplante. Métodos: Estudo observacional, transversal e analítico no qual o conjunto dos pacientes submetidos a transplante renal no estado de Rondônia, Brasil, foi avaliado por meio de entrevistas e análise de registros quanto a seu perfil clínico-demográfico e aos sinais e sintomas de disfunção miccional. Qui-quadrado, analysis of variance (ANOVA) e regressão linear multivariada foram utilizados para estabelecer possíveis correlações. Resultados: Abordaram-se 81,87% dos pacientes (n = 122). Os sintomas mais frequentes foram aumento da frequência (68,2%) e noctúria (97,6%). O teste ANOVA demonstrou relação entre a idade e o International Consultation on Incontinence Questionnaire (ICIQ) (Questionário Internacional de Consulta sobre Incontinência) (p = 0,014), etilismo e o Overactive Bladder Questionnaire (OAB-V8) (Questionário de Avaliação da Bexiga Hiperativa) (p = 0,001), esquema imunossupressor e ICIQ (p = 0,04), timoglobulina e OAB (p = 0.009), uso de duplo J e ICIQ (p = 0,014), infecção por citomegalovírus e OAB (p = 0,031) e International Prostate Symptom Score (IPSS) (Escore Internacional de Sintomas Prostáticos) (p = 0,008). Das comorbidades, foi observada insuficiência cardíaca relacionada aos escores OAB (p = 0,009) e ICIQ (p = 0,003). Qui-quadrado apontou associação entre uso de álcool e grupo 1 do OAB (p = 0,005). O teste de Pearson correlacionou positivamente tempo de isquemia do enxerto com ICIQ (p = 0,04). Conclusão: A prevalência de sintomas do trato urinário inferior é maior em transplantados renais do que na população geral, e foi possível determinar fatores preditivos para sua ocorrência. Objetivos Métodos observacional Rondônia Brasil clínicodemográfico clínico demográfico miccional Quiquadrado, Quiquadrado Qui quadrado, quadrado (ANOVA correlações Resultados Abordaramse Abordaram se 8187 81 87 81,87 n 122. 122 . 122) 68,2% 682 68 2 (68,2% 97,6%. 976 97,6% 97 6 (97,6%) (ICIQ Questionário Incontinência p 0,014, 0014 0,014 , 0 014 0,014) OABV8 OABV V8 V (OAB-V8 Hiperativa 0,001, 0001 0,001 001 0,001) 0,04, 004 0,04 04 0,04) 0.009, 0009 0.009 009 0.009) 0,031 0031 031 IPSS (IPSS Escore Prostáticos 0,008. 0008 0,008 008 0,008) comorbidades 0,009 0,003. 0003 0,003 003 0,003) 0,005. 0005 0,005 005 0,005) 0,04. Conclusão geral ocorrência 818 8 81,8 12 68,2 (68,2 97,6 9 (97,6% 0,01 01 (OAB-V 000 0,00 00 0,0 0.00 0,03 03 81, 68, (68, 97, (97,6 0, 0.0 (68 (97, 0. (6 (97 ( (9

https://doi.org/10.53855/bjt.v27i1.562_port

Brazilian consensus recommendations on the diagnosis and treatment of autoimmune encephalitis in the adult and pediatric populations
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Dutra, Lívia Almeida

; Silva, Pedro Victor de Castro

; Ferreira, João Henrique Fregadolli

; Marques, Alexandre Coelho

; Toso, Fabio Fieni

; Vasconcelos, Claudia Cristina Ferreira

; Brum, Doralina Guimarães

; Pereira, Samira Luisa dos Apóstolos

; Adoni, Tarso

; Rocha, Leticia Januzi de Almeida

; Sampaio, Leticia Pereira de Brito

; Sousa, Nise Alessandra de Carvalho

; Paolilo, Renata Barbosa

; Pizzol, Angélica Dal

; Costa, Bruna Klein da

; Disserol, Caio César Diniz

; Pupe, Camila

; Valle, Daniel Almeida do

; Diniz, Denise Sisterolli

; Abrantes, Fabiano Ferreira de

; Schmidt, Felipe da Rocha

; Cendes, Fernando

; Oliveira, Francisco Tomaz Meneses de

; Martins, Gabriela Joca

; Silva, Guilherme Diogo

; Lin, Katia

; Pinto, Lécio Figueira

; Santos, Mara Lúcia Schimtz Ferreira

; Gonçalves, Marcus Vinícius Magno

; Krueger, Mariana Braatz

; Haziot, Michel Elyas Jung

; Barsottini, Orlando Graziani Povoas

; Nascimento, Osvaldo José Moreira do

; Nóbrega, Paulo Ribeiro

; Proveti, Priscilla Mara

; Castilhos, Raphael Machado do

; Daccach, Vanessa

; Glehn, Felipe von

Arquivos de Neuro-Psiquiatria

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2024, Volume 82 Nº 7 elocation s00441788586

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background Autoimmune encephalitis (AIE) is a group of inflammatory diseases characterized by the presence of antibodies against neuronal and glial antigens, leading to subacute psychiatric symptoms, memory complaints, and movement disorders. The patients are predominantly young, and delays in treatment are associated with worse prognosis. Objective With the support of the Brazilian Academy of Neurology (Academia Brasileira de Neurologia, ABN) and the Brazilian Society of Child Neurology (Sociedade Brasileira de Neurologia Infantil, SBNI), a consensus on the diagnosis and treatment of AIE in Brazil was developed using the Delphi method. Methods A total of 25 panelists, including adult and child neurologists, participated in the study. Results The panelists agreed that patients fulfilling criteria for possible AIE should be screened for antineuronal antibodies in the serum and cerebrospinal fluid (CSF) using the tissue-based assay (TBA) and cell-based assay (CBA) techniques. Children should also be screened for anti-myelin oligodendrocyte glucoprotein antibodies (anti-MOG). Treatment should be started within the first 4 weeks of symptoms. The first-line option is methylprednisolone plus intravenous immunoglobulin (IVIG) or plasmapheresis, the second-line includes rituximab and/or cyclophosphamide, while third-line treatment options are bortezomib and tocilizumab. Most seizures in AIE are symptomatic, and antiseizure medications may be weaned after the acute stage. In anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis, the panelists have agreed that oral immunosuppressant agents should not be used. Patients should be evaluated at the acute and postacute stages using functional and cognitive scales, such as the Mini-Mental State Examination (MMSE), the Montreal Cognitive Assessment (MoCA), the Modified Rankin Scale (mRS), and the Clinical Assessment Scale in Autoimmune Encephalitis (CASE). Conclusion The present study provides tangible evidence for the effective management of AIE patients within the Brazilian healthcare system. (AIE antigens symptoms complaints disorders young prognosis Academia ABN Sociedade Infantil SBNI, SBNI , SBNI) method 2 neurologists CSF (CSF tissuebased tissue based TBA (TBA cellbased cell CBA (CBA techniques antimyelin anti myelin antiMOG. antiMOG MOG . (anti-MOG) firstline line IVIG (IVIG plasmapheresis secondline second andor cyclophosphamide thirdline third tocilizumab symptomatic stage antiNmethylDaspartate N methyl D aspartate antiNMDAR NMDAR (anti-NMDAR used scales MiniMental Mini Mental MMSE, MMSE (MMSE) MoCA, MoCA (MoCA) mRS, mRS (mRS) CASE. CASE (CASE) system (anti-MOG (MMSE (MoCA (mRS (CASE

Resumo Antecedentes Encefalites autoimunes (EAIs) são um grupo de doenças inflamatórias caracterizadas pela presença de anticorpos contra antígenos neuronais e gliais, que ocasionam sintomas psiquiátricos subagudos, queixas de memória e distúrbios anormais do movimento. A maioria dos pacientes é jovem, e o atraso no tratamento está associado a pior prognóstico. Objetivo Com o apoio da Academia Brasileira de Neurologia (ABN) e da Sociedade Brasileira de Neurologia Infantil (SBNI), desenvolvemos um consenso sobre o diagnóstico e o tratamento da EAIs no Brasil utilizando a metodologia Delphi. Métodos Um total de 25 especialistas, incluindo neurologistas e neurologistas infantis, foram convidados a participar. Resultados Os especialistas concordaram que os pacientes com critérios de possíveis EAIs devem ser submetidos ao rastreio de anticorpos antineuronais no soro e no líquido cefalorraquidiano (LCR) por meio das técnicas de ensaio baseado em tecidos (tissue-based assay, TBA, em inglês) e ensaio baseado em células (cell-based assay, CBA, em inglês). As crianças também devem ser submetidas ao rastreio de de anticorpo contra a glicoproteína da mielina de oligodendrócitos (anti-myelin oligodendrocyte glycoprotein, anti-MOG, em inglês). O tratamento deve ser iniciado dentro das primeiras 4 semanas dos sintomas, sendo as opções de primeira linha metilprednisolona combinada com imunoglobulina intravenosa (IGIV) ou plasmaférese. O tratamento de segunda linha inclui rituximabe e ciclofosfamida. Bortezomib e tocilizumab são opções de tratamento de terceira linha. A maioria das crises epilépticas nas EAIs são sintomáticas, e os fármacos anticrise podem ser desmamadas após a fase aguda. Em relação à encefalite antirreceptor de N-metil-D-aspartato (anti-N-methyl-D-aspartate receptor, anti-NMDAR, em inglês), os especialistas concordaram que agentes imunossupressores orais não devem ser usados. Os pacientes devem ser avaliados na fase aguda e pós-aguda mediante escalas funcionais e cognitivas, como Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Modified Rankin Scale (mRS), e Clinical Assessment Scale in Autoimmune Encephalitis (CASE). Conclusão Esta pesquisa oferece evidências tangíveis do manejo efetivo de pacientes com EAIs no sistema de saúde Brasileiro. (EAIs gliais subagudos movimento jovem prognóstico ABN (ABN SBNI, SBNI , (SBNI) Delphi 2 infantis participar LCR (LCR tissuebased tissue based assay TBA inglês cellbased cell CBA inglês. . antimyelin anti myelin glycoprotein antiMOG, antiMOG MOG, MOG anti-MOG IGIV (IGIV plasmaférese ciclofosfamida sintomáticas NmetilDaspartato N metil D aspartato antiNmethylDaspartate methyl aspartate receptor antiNMDAR, antiNMDAR NMDAR, NMDAR anti-NMDAR inglês, usados pósaguda pós cognitivas MiniMental Mini Mental MMSE, MMSE (MMSE) MoCA, MoCA (MoCA) mRS, mRS (mRS) CASE. CASE (CASE) Brasileiro (SBNI (MMSE (MoCA (mRS (CASE

https://doi.org/10.1055/s-0044-1788586

Cell sheet produced from periodontal ligament stem cells activated by PAR1 improves osteogenic differentiation PAR
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

GASPARONI, Letícia Miquelitto

; ALVES, Tomaz

; FRANÇA, Bruno Nunes de

; BALZARINI, Danilo

; ALBUQUERQUE-SOUZA, Emmanuel

; PEDRONI, Ana Clara Fagundes

; ROVAI, Emanuel da Silva

; MENDOZA, Aldrin Huamán

; SIPERT, Carla Renata

; HOLZHAUSEN, Marinella

Brazilian Oral Research

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2024, Volume 38 elocation e079

Resumo: En | Texto: En | PDF: En

Abstract Periodontal regeneration is a challenge, and tissue engineering based on periodontal ligament stem cells (PDLSCs) has been shown to be a promising alternative to this process. However, the need for scaffolds has limited the therapeutic use of PDLSCs. In this context, scaffold-free tissue engineering using the cell sheet (CS) technique has been developed as an alternative approach to improve tissue regeneration. Previously, we showed that Protease-activated receptor-1 (PAR1) can regulate PDLSCs. Herein, we evaluate whether PAR1 influences osteogenesis in CSs produced from PDLSCs, without the use of scaffolds. PDLSCs were isolated and immunophenotyped. Then, CSs were obtained by supplementing the culture medium with ascorbic acid (50 µg/mL), and PAR1 was activated through its agonist peptide (100 nM). Scaffold-free 3D CSs were successfully produced from PDLSCs, and they showed higher proliferation potential than isolated PDLSCs. Also, PAR1 activation decreased senescence and improved osteogenic differentiation of CSs by increasing mineralized nodule deposition and alkaline phosphatase concentration; PAR1 also modulated osteogenic markers at the gene and protein levels. We further demonstrated that this effect was regulated by Wnt, TGF-βI, MEK, p38 MAPK, and FGF/VEGF signaling pathways in PDLSCs (p < 0.05%). Overall, PAR1 activation increased osteogenic activity in CSs, emerging as a promising scaffold-free therapeutic approach for periodontal regeneration. challenge (PDLSCs process However context scaffoldfree scaffold free CS (CS Previously Proteaseactivated Protease receptor1 receptor 1 receptor- PAR (PAR1 Herein immunophenotyped Then 50 (5 µg/mL, µgmL µg/mL , µg mL µg/mL) 100 (10 nM. nM . nM) Scaffoldfree Scaffold D Also concentration levels Wnt TGFβI, TGFβI TGF βI, βI TGF-βI MEK p p3 MAPK FGFVEGF FGF VEGF 0.05%. 005 0.05% 0 05 0.05%) Overall (PAR 5 ( 10 (1 00 0.05 0.0 0.

https://doi.org/10.1590/1807-3107bor-2024.vol38.0079

Do YouTube à notícia: vulnerabilidade e agência nas representações de crianças produtoras de conteúdo notícia
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Tomaz, Renata Oliveira

Galáxia (São Paulo)

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 48 elocation e58837

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract This article originates from research that investigates the relationship between visibility, childhood and participation on the internet. This paper analyzes the representations of children youtubers in a sample of 43 pieces of news collected through an alert of Google searcher between 2017 and 2019. Based on understanding of news as a cultural product and on children's visibility as a social image of kids, this work’s methodology is based on the principles of content analysis. The partial findings indicate that the protagonism that kids have in social media, particularly on YouTube, is problematized by the analyzed news content, generating social repercussions that do not celebrate such protagonism, and also do not fail to recognize children as subjects. internet 4 201 2019 childrens s works work analysis media YouTube subjects 20 2

Resumo Este texto se origina de pesquisa que investiga a relação entre visibilidade, infância e participação na internet. O estudo analisa as representações de youtubers crianças em uma amostra de 43 notícias coletadas por meio de um alerta do buscador Google, entre os anos de 2017 e 2019. Com base na compreensão da notícia como produto cultural e na visibilidade infantil como imagem social das crianças, a metodologia do trabalho se valeu dos princípios da análise de conteúdo. Os achados parciais indicam que o protagonismo infantil de que gozam nas mídias sociais, particularmente no YouTube, é problematizado pelo conteúdo noticioso analisado, gerando repercussões sociais que, embora não celebrem tal protagonismo, não deixam de reconhecer a condição de sujeito das crianças. internet 4 Google 201 2019 YouTube analisado 20 2

https://doi.org/10.1590/1982-2553202358837

Inheritance of soluble solids content and sucrose in melon
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Silva, Juliana Maria C da

; Viana, Elaine Renata de C

; Morais, Patrícia Lígia D de

; Tomaz, Francisco L de S

; Martins, Adriano F

; Nunes, Glauber Henrique de S

Horticultura Brasileira

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Set 2022, Volume 40 Nº 3 Páginas 295 - 301

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Os principais parâmetros de qualidade do fruto de melão relacionam-se com o acúmulo de sacarose e o teor de sólidos solúveis. O conhecimento do controle genético desses caracteres é fundamental para auxiliar o melhorista no processo seletivo. Objetivou-se com este trabalho estudar a herança do acúmulo de sacarose e do teor de sólidos solúveis em melão. Utilizou-se o delineamento em blocos casualizados com três repetições para avaliar os genitores AC-16 e Vedrantais, e as gerações F1, F2, RC1 e RC2. Verificou-se que a sacarose é o principal fator que confere a variabilidade genética e ambiental observada no teor de açúcar entre os genitores. A herança do teor de sacarose envolve um gene de efeito maior com efeitos aditivos e de dominância associado aos poligenes com efeitos aditivos. A herança de sólidos solúveis envolve um gene maior com efeitos aditivos e de dominância associado aos poligenes com efeitos aditivos e a presença de epistasia.

ABSTRACT The main quality parameters of melon are related to sucrose accumulation and soluble solids content. Understanding the genetic control of these traits is essential to help breeders in the selection process. The aim of this study was to evaluate the inheritance of sucrose accumulation and soluble solids content in melon. A randomized block design with three replicates was used to evaluate AC-16 and Vedrantais parents, and F1, F2, BC1 and BC2 generations. We verified that sucrose is the main factor which is related to genetic and environmental variability observed in sugar content between parents. The inheritance of sucrose content involves a major effect gene with additive and dominance effects associated with polygenes with additive effects. The inheritance of soluble solids involves a major gene with additive and dominance effects associated with polygenes, with additive effects, and the presence of epistasis.

https://doi.org/10.1590/s0102-0536-20220308

Inheritance of beta-carotene content in melon
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Silva, Juliana Maria Costa da

; Viana, Elaine Renata de Castro

; Chagas, Paulo Sérgio Fernandes das

; Dombroski, Jeferson Luiz Dallabona

; Moraes, Patrícia Lígia Dantas de

; Tomaz, Francisco Linco de Souza

; Nunes, Glauber Henrique de Sousa

Pesquisa Agropecuária Brasileira

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2022, Volume 57 elocation e02833

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste trabalho foi determinar a herança do teor de betacaroteno em melão (Cucumis melo). O acesso AC-16 (Cucumis melo subsp. melo var. acidulus) – com baixo teor de betacaroteno e mesocarpo branco – foi cruzado com a cultivar Vedrantais (C. melo subsp. melo var. cantalupensis) – com alto teor de betacaroteno e mesocarpo de cor salmão –, para obtenção das gerações F1, F2, RC1 e RC2. Avaliaram-se os genitores AC-16 e 'Vedrantais', as gerações F1 e F2, e os retrocruzamentos de cada genitor RC1 e RC2. A quantificação do betacaroteno foi realizada em sistema de cromatografia líquida de alto desempenho. Foram estimados os componentes de média relacionados aos efeitos aditivos e de dominância, as variâncias aditiva e de dominância e a herdabilidade. O teor de betacaroteno foi alto (17,78 µg g-1) em 'Vedrantais' e baixo em AC-16 (0,34 µg g-1). Observaram-se os seguintes resultados: efeito aditivo e de dominância no controle genético do caráter, dominância de caráter incompleta, número estimado de loci próximo de dois, maior variância para populações segregantes (F2 e retrocruzamentos), e valores de herdabilidade nos sentidos amplo (87,75%) e restrito (64,19%). O teor de betacaroteno em melão é controlado por um gene de efeito maior, com efeitos aditivos e de dominância associados a poligenes com efeitos aditivos.

Abstract The objective of this work was to determine the inheritance of beta-carotene content in melon (Cucumis melo). The AC-16 accession (Cucumis melo subsp. melo var. acidulus) – with a low beta-carotene content and white mesocarp – was crossed with the Vedrantais cultivar (C. melo subsp. melo var. cantalupensis) – with a high beta-carotene content and salmon colored mesocarp –, to obtain the F1, F2, BC1, and BC2 generations. The AC-16 and 'Vedrantais' parents, the F1 and F2 generations, and the BC1 and BC2 backcrosses of each parent were evaluated. The quantification of beta-carotene was carried out in a high-performance liquid chromatography system. Mean components related to the additive and dominance effects, additive and dominance variances, and heritability were estimated. The beta-carotene content was high in 'Vedrantais' (17.78 µg g-1) and low in AC-16 (0.34 µg g-1). The following results were observed: additive and dominance effects on the genetic control of the character, incomplete character dominance, estimated number of loci close to two, greater variance for segregating populations (F2 and backcrosses), and heritability values in the broad (87.75%) and narrow (64.19%) senses. The beta-carotene content in melon is controlled by a major effect gene, with additive and dominance effects associated with polygenes with additive effects.

https://doi.org/10.1590/s1678-3921.pab2022.v57.02833

Cytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cells
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Pedrosa, Marlus da Silva

; Alves, Tomaz

; Rahhal, Juliana Garuba

; Nogueira, Fernando Neves

; Sipert, Carla Renata

Pesquisa Brasileira em Odontopediatria e Clínica Integrada

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2022, Volume 22 elocation e210114

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods The culture of hPDLSCs was established. Cell density was set at 2 × 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 1:4 and 1:16) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey’s test (α=0.05). Results At 24 h, pure extract of MTA Repair HP and Biodentine 1:16 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 1:4 and 1:16, and White MTA 1:16. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs.

https://doi.org/10.1590/pboci.2022.004

10.

Protease-activated receptor type 1 (PAR1) increases CEMP1 gene expression through MAPK/ERK pathway
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Rovai, Emanuel Silva

; Alves, Tomaz

; Gasparoni, Letícia Miquelitto

; França, Bruno Nunes de

; Sipert, Carla Renata

; Kantarci, Alpdogan

; Holzhausen, Marinella

Brazilian Oral Research

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2022, Volume 36 elocation e048

Resumo: En | Texto: En | PDF: En

Abstract: PAR1 is a G-coupled protein receptor that regulates several cellular metabolism processes, including differentiation and proliferation of osteogenic and cementogenic related cells and our group previously demonstrated the regenerative potential of PAR1 in human periodontal ligament stem cells (hPDLSCs). In this study, we hypothesized that PAR1 regulates the cementogenic differentiation of hPDLSCs. Our goal was to identify the intracellular signaling pathway underlying PAR1 activation in hPDSLC differentiation. hPDLSCs were isolated using the explant technique. Cells were cultured in an osteogenic medium (OST) (α-MEM, 15% fetal bovine serum, L-glutamine, penicillin, streptomycin, amphotericin B, dexamethasone, and beta-glycerophosphate). The hPDLSCs were treated with a specific activator of PAR1 (PAR1 agonist) and blockers of the MAPK/ERK and PI3K pathways for 2 and 7 days. The gene expression of CEMP1 was assessed by RT-qPCR. The activation of PAR1 by its agonist peptide led to an increase in CEMP1 gene expression when compared with OST control. MAPK/ERK blockage abrogated the upregulation of CEMP1 gene expression induced by PAR1 agonist (p < 0.05). PI3K blockage did not affect the gene expression of CEMP1 at any experimental time (p > 0.05). We concluded that CEMP1 gene expression increased by PAR1 activation is MAPK/ERK-dependent and PI3K independent, suggesting that PAR1 may regulate cementogenetic differentiation of hPDLSCs.

https://doi.org/10.1590/1807-3107bor-2022.vol36.0048

11.

Cardiac Remodeling in Obesity-Resistance Model is not Related to Collagen I and III Protein Expression ObesityResistance Obesity Resistance
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Oliveira, Scarlet Marques de

; Garcia, Jéssica Leite

; Vileigas, Danielle Fernandes

; Campos, Dijon Henrique Salomé de

; Francisqueti-Ferron, Fabiane Valentini

; Ferron, Artur Junio Togneri

; Silva-Bertani, Danielle Cristina Tomaz da

; Padovani, Carlos Roberto

; Corrêa, Camila Renata

; Cicogna, Antonio Carlos

International Journal of Cardiovascular Sciences

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Dez 2021, Volume 34 Nº 6 Páginas 656 - 664

Resumo: En | Texto: En | PDF: En

Abstract Background: As some individuals present resistance to obesity development, experiments have been trying to understand their susceptibility to cardiometabolic diseases. Objetive: To evaluate if the cardiac remodeling was related to collagen protein expression change. Methods: Male Wistar rats were randomized into two experimental groups: control diet (CD, n=15) or high-fat diet (HFD, n=15) for 30 weeks. Rats fed with HFD were ranked based on their adiposity indexes and classified as obese (Ob, n = 8) or obesity-resistant (ROb, n = 6). Rats that failed to present the normal characteristic of the control group while fed with CD were excluded (Control, n = 8). Nutritional profile, comorbidities (dyslipidemia, hypertension, glucose metabolism, hyperleptinemia), cardiac remodeling, and collagen protein expression were evaluated. The groups were compared by One-Way ANOVA, together the Tukey post hoc test, with p<0.05 considered significant. Results: The Ob rats presented an increased adiposity index when compared to C and ROb. Both groups Ob and ROb presented increased low-density lipoprotein (LDL), insulin, homeostatic model assessment of insulin resistance (HOMA- IR) and systolic blood pressure (SBP), and low high-density lipoprotein (HDL) levels when compared to the control group. The levels of triglycerides, non-esterified fatty acid (NEFA), and leptin were lower in ROb as compared to Ob, but higher than the control group. The Ob and ROb groups presented cardiac remodeling, evidenced by echocardiographic and post-mortem analysis. The collagen protein expression did not differ among the groups. Conclusion: The ROb animals present cardiac remodeling that is not related to collagen type I and III protein expression change. Background development diseases Objetive change Methods CD, (CD n=15 n15 15 highfat high fat HFD, (HFD 3 weeks (Ob 8 obesityresistant resistant ROb, (ROb 6. 6 . 6) Control, Control (Control 8. profile dyslipidemia, dyslipidemia (dyslipidemia hypertension metabolism hyperleptinemia, hyperleptinemia , hyperleptinemia) evaluated OneWay One Way ANOVA test p005 p 0 05 p<0.0 significant Results lowdensity density LDL, LDL (LDL) HOMA (HOMA IR SBP, SBP (SBP) highdensity HDL (HDL triglycerides nonesterified non esterified NEFA, NEFA (NEFA) postmortem mortem analysis Conclusion n=1 n1 1 p00 p<0. (LDL (SBP (NEFA n= p0 p<0 p<

https://doi.org/10.36660/ijcs.20200058

12.

Cytotoxicity and cytokine production by calcium silicate-based materials on periodontal ligament stem cells
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Pedrosa, Marlus da Silva

; Alves, Tomaz

; Nogueira, Fernando Neves

; Holzhausen, Marinella

; Sipert, Carla Renata

Brazilian Dental Journal

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jun 2021, Volume 32 Nº 3 Páginas 65 - 74

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Este estudo investigou o efeito de três materiais comerciais à base de silicato de cálcio (CSBM) na citotoxicidade e na produção de citocinas pró e antiinflamatórias em células-tronco do ligamento periodontal humano (hPDLSCs). Cultura de hPDLSCs foi estabelecida e caracterizada. Extratos de Bio-C Sealer (Angelus, Londrina, PR, Brasil), MTA Fillapex (Angelus, Londrina, PR, Brasil) e PBS Cimmo HP® (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brasil) foram preparados com a colocação de espécimes dos cimentos (5 x 3 mm) em meio de cultura. Em seguida, os extratos foram diluídos (1, 1: 2, 1: 4, 1: 8, 1:16) e inseridos nos poços semeados de células para ensaio de citotoxicidade por meio de MTT por 24, 48 e 72 h. As citocinas TNF-α e IL-10 foram quantificadas por ELISA em sobrenadantes de células de 24 h. Os dados foram analisados por ANOVA e teste de Tukey (α = 0,05). Todos os CSBM exibiram alguma citotoxicidade que variou de acordo com a concentração do extrato e o tempo de avaliação. O MTA Fillapex apresentou os maiores efeitos citotóxicos com redução significativa da atividade metabólica / viabilidade celular quando comparado ao Bio-C Sealer e Cimmo HP®. O TNF-α foi regulado positivamente pelos três cimentos testados (p <0,05), enquanto apenas o MTA Fillapex regulou positivamente a liberação de IL-10 em comparação com o controle. Tomados em conjunto, os resultados mostraram que PBS Cimmo HP®, Bio-C Sealer e MTA Fillapex apresentam citotoxicidade leve e transitória e induziram a produção de TNF-α. O MTA Fillapex regulou positivamente a liberação de IL-10 por hPDLSCs.

Abstract This study investigated the effect of three commercial calcium silicate-based materials (CSBM) on cytotoxicity and pro-and anti-inflammatory cytokines production in cultured human periodontal ligament stem cells (hPDLSCs). Culture of hPDLSCs was established and characterized. Extracts of Bio-C Sealer (Angelus, Londrina, PR, Brazil), MTA Fillapex (Angelus, Londrina, PR, Brazil) and PBS Cimmo HP (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brazil) were prepared by placing cement specimens (5 x 3 mm) in culture medium. Then, the extracts were serially two-fold diluted (1, 1:2, 1:4, 1:8, 1:16) and inserted into the cell-seeded wells for 24, 48 and 72 h for MTT assays. TNF-α and IL-10 cytokines were quantified by ELISA at 24h-cell supernatants. Data were analyzed by ANOVA and Tukey’s test (α = 0.05). All CSBM exhibited some cytotoxicity that varied according to extract concentration and time of evaluation. MTA Fillapex presented the highest cytotoxic effects with significant reduction of metabolic activity/cell viability when compared to Bio-C Sealer and Cimmo HP®. TNF-α was significantly upregulated by the three tested cements (p < 0.05) while only MTA Fillapex significantly upregulated IL-10 in comparison to control. Taken collectively, the results showed that PBS Cimmo HP®, Bio-C Sealer and MTA Fillapex present mild and transient cytotoxicity and slightly induced TNF-α production. MTA Fillapex upregulated IL-10 release by hPDLSCs.

https://doi.org/10.1590/0103-6440202104467 38 downloads

13.

APLICABILIDADE DE UM QUESTIONÁRIO GENÉRICO DE AVALIAÇÃO DE QUALIDADE DE VIDA PARA CRIANÇAS ASMÁTICAS
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Silva, Ana Aline Marcelino da

; Maciel, Álvaro Campos Cavalcanti

; Furtado, Priscilla Rique

; Tomaz, Renata Ramos

; Macêdo, Thalita Medeiros Fernandes de

; Mendonça, Karla Morganna Pereira Pinto de

Revista Paulista de Pediatria

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jun 2018, Volume 36 Nº 2 Páginas 207 - 213

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Avaliar se o instrumento genérico Pediatric Quality of Life Inventory (PedsQL 4.0) é aplicável à avaliação da qualidade de vida relacionada à saúde (QVRS) de crianças asmáticas, quando comparado ao instrumento específico Pediatric Asthma Quality of Life Questionnaire (PAQLQ). Métodos: Foram avaliadas 30 crianças com idade entre sete e onze anos de idade e diagnóstico de asma havia, pelo menos, seis meses. Ambos os questionários foram respondidos no mesmo dia, por meio de entrevista. Os dados foram analisados com o auxílio do Statistical Package for the Social Sciences (SPSS), versão 20.0, atribuindo-se o nível de significância de 5%. Resultados: Não ocorreram diferenças significativas na qualidade de vida das crianças avaliadas de acordo os sexos. As crianças asmáticas classificadas como praticantes e não praticantes de atividade física obtiveram escores significativamente diferentes no domínio relacionado à saúde física (PedsQL 4.0). Crianças com diferentes níveis de gravidade e controle da asma apresentaram diferenças significativas nos escores obtidos em dois dos três domínios avaliados por meio do PAQLQ. Ao serem avaliadas com o instrumento PedsQL 4.0, nenhuma diferença significativa foi observada na qualidade de vida das crianças com diferentes níveis de controle e gravidade da asma. As medidas de qualidade de vida de crianças asmáticas obtidas pelos instrumentos PedsQL 4.0 e PAQLQ apresentaram correlação moderada e significativa (r=0,415, p=0,02). Conclusões: O instrumento PedsQL 4.0 diferenciou as crianças com diferentes níveis de atividade física. No entanto, ele não foi sensível para diferenciar a qualidade de vida relacionada à saúde em crianças com diferentes níveis de gravidade e controle da asma.

ABSTRACT Objective: To determine the applicability of the generic instrument Pediatric Quality of Life Inventory (PedsQL 4.0) to assess health-related quality of life of asthmatic children compared to the specific instrument Pediatric Asthma Quality of Life Questionnaire (PAQLQ). Methods: This research involved the evaluation of 30 children aged seven to eleven years, who had been diagnosed with asthma for at least six months prior to research. Both quality of life questionnaires were applied to children by an interviewer over the period of one day. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) 20.0, with significance level set at 5%. Results: No differences in quality of life was found when genders were compared. Asthmatic children classified as practitioners and non-practitioners of physical activity had different scores in the physical health-related domain (PedsQL 4.0). The scores of children with different levels of asthma severity and control were significantly different in two out of three domains evaluated by PAQLQ. When assessed by PedsQL 4.0, no significant difference was observed as to quality of life of children with different levels of asthma control and severity. Quality of life measurements of asthmatic children by PedsQL 4.0 and PAQLQ instruments had a moderate and significant correlation (r=0.415, p=0.02). Conclusions: PedsQL 4.0 could distinguish children practicing different levels of physical activity, but it was not sensitive enough to distinguish health-related quality of life among children with different levels of asthma severity and control.

https://doi.org/10.1590/1984-0462/;2018;36;2;00006 1663 downloads

14.

Artificial neural network for prediction of the area under the disease progress curve of tomato late blight
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Alves, Daniel Pedrosa

; Tomaz, Rafael Simões

; Laurindo, Bruno Soares

; Laurindo, Renata Dias Freitas

; Silva, Fabyano Fonseca e

; Cruz, Cosme Damião

; Nick, Carlos

; Silva, Derly José Henriques da

Scientia Agricola

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Fev 2017, Volume 74 Nº 1 Páginas 51 - 59

Resumo: En | Texto: En | PDF: En

ABSTRACT: Artificial neural networks (ANN) are computational models inspired by the neural systems of living beings capable of learning from examples and using them to solve problems such as non-linear prediction, and pattern recognition, in addition to several other applications. In this study, ANN were used to predict the value of the area under the disease progress curve (AUDPC) for the tomato late blight pathosystem. The AUDPC is widely used by epidemiologic studies of polycyclic diseases, especially those regarding quantitative resistance of genotypes. However, a series of six evaluations over time is necessary to obtain the final area value for this pathosystem. This study aimed to investigate the utilization of ANN to construct an AUDPC in the tomato late blight pathosystem, using a reduced number of severity evaluations. For this, four independent experiments were performed giving a total of 1836 plants infected with Phytophthora infestans pathogen. They were assessed every three days, comprised six opportunities and AUDPC calculations were performed by the conventional method. After the ANN were created it was possible to predict the AUDPC with correlations of 0.97 and 0.84 when compared to conventional methods, using 50 % and 67 % of the genotype evaluations, respectively. When using the ANN created in an experiment to predict the AUDPC of the other experiments the average correlation was 0.94, with two evaluations, 0.96, with three evaluations, between the predicted values of the ANN and they were observed in six evaluations. We present in this study a new paradigm for the use of AUDPC information in tomato experiments faced with P. infestans. This new proposed paradigm might be adapted to different pathosystems.

https://doi.org/10.1590/1678-992x-2015-0309 2447 downloads

15.

Feminismo, maternidade e mídia: relações historicamente estreitas em revisão
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Tomaz, Renata

Galáxia (São Paulo)

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jun 2015, Nº 29 Páginas 155 - 166

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo:Partindo do princípio de que a maternidade é uma construção social cada vez mais vinculada aos processos comunicacionais, sobretudo na voluptuosa oferta de conselhos e diretrizes do que é ser mãe, este trabalho procura situar tal debate com base nas principais abordagens feitas sobre maternidade nos estudos feministas. O trabalho apresenta um recorte do estado dessa pesquisa no Brasil, chamando atenção para três eixos temático-teóricos e para os possíveis pontos de exploração nos estudos de mídia e maternidade.

Abstract:We start from the premise that motherhood is a social construct increasingly linked to communication processes, especially in the voluptuous offer of advice and guidelines of what is to be a mother. Taking on from this idea, this paper seeks to situate such debate. Based on the main approaches about motherhood in feminist studies, the paper presents a state-focused approach of this research in Brazil, bringing attention to three thematic-theoretical axes and to potential topics yet to be explored in media and motherhood studies.

https://doi.org/10.1590/1982-25542015120031 5410 downloads

Exibindo

itens por página

Página de 2 Próxima

Selecione um ou mais resultados

Todos Ordem alfabética

Resultados

Gráfico comparativo Download CSV

Visualizar estatísticas de

Enviar resultado

E-mail do(s) destinatários*

Utilize ; (ponto e vírgula) para mais de um e-mail.

Enviar por e-mail

Esta página

Registros selecionados

Todos os registros (max. 300)

Alterar remetente, assunto e comentários

Seu email

Assunto

Comentários

Alterar remetente, assunto e comentários

Exportar resultados

Formato de exportação:

RIS (para EndNote, ProCite, Reference Manager, RefWorks, Citavi, etc.)

BibTeX

Citação

CSV (para Excel, etc)

Exportar:

Esta página

Itens selecionados

Todos os registros (max. 2000)

Mostrar mais

Sem resultados

Não foram encontrados documentos para sua pesquisa

Glossário e ajuda para busca

Você pode enriquecer sua busca de uma forma muito simples. Use os índices de pesquisa combinados com os conectores (AND ou OR) e especifique cada vez mais sua busca.

Por exemplo, se você deseja buscar artigos sobre
casos de dengue no Brasil em 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

Veja abaixo a lista completa de índices de pesquisa que podem ser usados:

Cód. do Índice	Elemento
ti	título do artigo
au	autor
kw	palavras-chave do artigo
subject	assunto (palavras do título, resumo e palavras-chave)
ab	resumo
ta	título abreviado da revista (ex. Cad. Saúde Pública)
journal_title	título completo da revista (ex. Cadernos de Saúde Pública)
la	código do idioma da publicação (ex. pt - Português, es - Espanhol)
type	tipo do documento
pid	identificador da publicação
publication_year	ano de publicação do artigo
sponsor	financiador
aff_country	código do país de afiliação do autor
aff_institution	instituição de afiliação do autor
volume	volume do artigo
issue	número do artigo
elocation	elocation
doi	número DOI
issn	ISSN da revista
in	código da coleção SciELO (ex. scl - Brasil, col - Colômbia)
use_license	código da licença de uso do artigo

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
Email: scielo@scielo.org

Todo o conteúdo de www.scielo.org, exceto onde está identificado, está licenciado sob uma Licença Creative Commons.
saiba mais »