To discover new bisamide compounds with insecticidal and fungicidal activities, 19 novel cyproflanilide derivatives containing 1,2,4-oxadiazole group were designed and synthesized according to the principle of biologically active factor splicing. Preliminary biological assay data indicated that the majority of target compounds demonstrated significant insecticidal activity against Lepidopteran pests. Meanwhile, compounds 5p and 5q showed 97.22 and 100% lethality against Tetranychus cinnabarinus at 400 mg L-1, which were better than cyproflanilide (41.11% at 400 mg L-1). In addition, it is exciting that we have achieved our initial goals as some of the target compounds exhibited both insecticidal and fungicidal activities. For instance, compounds 5b and 5c showed 59.26 and 74.07% fungicidal activities against Cucumber downy mildew at 400 mg L-1, as well as 100% insecticidal activity against Plutella xylostella and armyworm at 100 mg L-1. Compound 5h showed 100% insecticidal activity against armyworm at 10 mg L-1, and also displayed 66.67% fungicidal activity against Cucumber downy mildew at 400 mg L-1. The inhibition rate of compounds 5l and 5s against Cucumber downy mildew reached 88.89 and 96.30% at 400 mg L-1. This work showed the effectively fungicidal application of 1,2,4-oxadiazole group in bisamide compounds and provides insights for optimal structural design. 1 1,2,4oxadiazole 124oxadiazole oxadiazole 1,2,4 2 4 splicing pests Meanwhile p q 9722 97 22 97.2 40 L1, L1 L 1, L-1 41.11% 4111 41 11 (41.11 L1. . L-1) addition instance b c 5926 59 26 59.2 7407 74 07 74.07 1. h 6667 66 67 66.67 l s 8889 88 89 88.8 9630 96 30 96.30 design 4oxadiazole 124 1,2, 972 9 97. L- 41.11 411 (41.1 592 5 59. 740 7 0 74.0 666 6 66.6 888 8 88. 963 3 96.3 12 1,2 41.1 (41. 74. 66. 96. 41. (41 (4 (

The recycle and utilization of expired drugs is a challenge for many countries at present. The employment of expired drugs as green corrosion inhibitors is expected to address both drug recycling and corrosion prevention issues, with significant economic and environmental value. Herein, the individually and combination corrosion inhibition performance of expired amoxicillin (AMC) and penicillin V potassium tablets (PVP) for lowcarbon steel in 20% acetic acid solution containing 600 mg L-1 chloride ion are evaluated by electrochemistry and weight loss method. Both the AMC and PVP present the mixed-type corrosion inhibitors mainly affect the cathode process when used individually. The optimum inhibition efficiency is 61.22% (AMC) and 79.21% (PVP), which all appear in 5.0 × 10-3 mol L-1. The AMC and PVP are adsorbed on the steel surface by mixed physisorption and chemisorption, which are consistent with the Langmuir adsorption isotherm. The corrosion inhibition rate drops sharply when AMC and PVP are combined use with a close mole ratio. But a small amount of PVP significantly enhance the corrosion inhibition effect of AMC, the corrosion inhibition rate from 61.22 to 79.72%. The corrosion inhibition rate of AMC and PVP combination inhibitor decreased with the increase of temperature. issues value Herein (AMC (PVP 20 60 L1 L 1 L- method mixedtype type 6122 61 22 7921 79 21 79.21 PVP, , 50 5 0 5. 103 10 3 10- L1. 1. chemisorption isotherm ratio 61.2 7972 72 79.72% temperature 2 6 612 792 7 79.2 61. 797 79.72 79. 79.7

ABSTRACT: Pork price fluctuations are closely related to the national economy and people’s livelihoods in China. Based on the monthly pork price fluctuations in China from January 2011 to August 2022, this study uses ARCH family models to assess the characteristics and laws of these fluctuations in China. The pork price fluctuations show obvious clustering, with external shock information from the previous month affecting the pork price in the following period; the pork market price is characterized by risk compensation, with the high risk of pork supply driving the pork price up. In addition, the pork price fluctuations are characterized by asymmetry, with a greater impact of good than of bad news on the pork price. Due to the pork industry’ low entry threshold and the existence of sunk costs, positive information on the pork market has a stronger impact on price fluctuations than negative information. To guide pork supply, we recommend improving monitoring and early-warning mechanisms in the pork market to identify the pork price volatility threshold and measure the price volatility. In addition, price index insurance products should be constantly strengthened, with different types of insurance products being offered to meeting the insurance demand of various sectors in the pig meat supply chain. ABSTRACT peoples people s 201 2022 clustering period compensation up addition asymmetry industry costs earlywarning early warning strengthened chain 20 202 2

RESUMO: As flutuações do preço da carne suína estão intimamente relacionadas à economia nacional e à subsistência das pessoas. Com base na flutuação mensal do preço da carne suína na China de a janeiro de 2011 a agosto de 2022, este estudo usa os modelos da família ARCH para avaliar as características e as leis das flutuações do preço da carne suína na China. Nossos estudos mostraram que as flutuações do preço da carne suína apresentam agrupamento óbvio, e as informações de choque externo do mês anterior irão interferir no preço da carne suína no período seguinte. O preço de mercado da carne suína tem as características de “risco de recompensa”, e o “alto risco” do preço da carne suína. Observa-se uma tendência de alta, mostrando que as oscilações do preço da carne suína são caracterizadas por assimetria. Devido ao baixo limiar de entrada da indústria de suínos e à existência de “sinking cost”, as informações positivas sobre o mercado de suínos têm um impacto mais forte nas flutuações de preços do que as informações negativas. Recomenda-se melhorar o monitoramento do mercado de carne suína e o mecanismo de alerta precoce, esclarecer e identificar o limite de volatilidade do preço da carne suína e melhorar o sistema de seguro financeiro da indústria suína. RESUMO pessoas 201 2022 óbvio seguinte risco recompensa, recompensa , recompensa” alto Observase Observa se alta assimetria sinking cost, cost cost” negativas Recomendase Recomenda precoce 20 202 2

ABSTRACT Objective: The objective of this study was to evaluate the impact of drainage tube placement on postoperative pain, recovery, and opioid consumption within a 72-hour period following unicompartmental knee arthroplasty (UKA). Methods: Patients with medial knee osteoarthritis who underwent UKA from January 2019 to August 2020 were enrolled in the study and divided into two groups based on whether they received a drain postoperatively. Results: The drainage group had significantly lower VAS scores on day 1, day 2, and day 3, in addition to significantly smaller changes in the circumference of the knee joint within 3 days postoperatively (P <0.05). The ROM in the drainage group significantly increased at 3 days and 1 month post-surgery, with a statistically significant difference in morphine consumption between the two groups at 3 days (P<0.05). The incidence of postoperative nausea and vomiting (5 cases) and wound bleeding (1 case) was lower in the drainage group compared to the non-drainage group (P<0.05). Conclusions: The placement of a drainage tube in UKA may reduce the swelling of knee joint and pain, which not only reduces the use of Opioid but also facilitates early functional activities of the knee joint. Level of Evidence III; Retrospective Comparative Study. Objective pain recovery 72hour hour 72 UKA. . (UKA) Methods 201 202 Results 2 P <0.05. 005 <0.05 0 05 <0.05) postsurgery, postsurgery post surgery, surgery post-surgery P<0.05. P005 P<0.05 (P<0.05) 5 ( cases case nondrainage non Conclusions III Study 7 (UKA 20 00 <0.0 P00 P<0.0 (P<0.05 <0. P0 P<0. (P<0.0 <0 P<0 (P<0. < P< (P<0 (P<

RESUMO Objetivo: O objetivo deste estudo foi avaliar o impacto da implantação do tubo de drenagem na dor pós-operatória, na recuperação e no consumo de opioides em um período de 72 horas após a artroplastia unicompartimental do joelho (UKA). Métodos: Pacientes com osteoartrite medial do joelho submetidos à UKA de janeiro de 2019 a agosto de 2020 foram incluídos no estudo e divididos em dois grupos com base no fato de terem ou não recebido um dreno no pós-operatório. Resultados: O grupo de drenagem apresentou escores EVA significativamente menores no dia 1, no dia 2 e no dia 3, além de alterações significativamente menores na circunferência da articulação do joelho em 3 dias de pós-operatório (P <0,05). A ADM no grupo de drenagem aumentou significativamente em 3 dias e 1 mês após a cirurgia, com uma diferença estatisticamente significativa no consumo de morfina entre os dois grupos em 3 dias (P<0,05). A incidência de náuseas e vômitos no pós-operatório(5 casos) e sangramento da ferida (1 caso) foi menor no grupo de drenagem em comparação com o grupo sem drenagem (P<0,05). Conclusão: A utilização de tubo de drenagem na UKA pode reduzir o edema articular do joelho e a dor, reduzindo o uso de opioides e facilitando as atividades funcionais iniciais da articulação do joelho. Nível de Evidência III; Estudo Comparativo Retrospectivo. Objetivo pósoperatória, pósoperatória pós operatória, operatória pós-operatória 7 UKA. . (UKA) Métodos 201 202 pósoperatório. pósoperatório operatório. operatório Resultados P <0,05. 005 <0,05 0 05 <0,05) cirurgia P<0,05. P005 P<0,05 (P<0,05) pósoperatório5 5 pós-operatório( casos ( caso Conclusão III Retrospectivo (UKA 20 00 <0,0 P00 P<0,0 (P<0,05 <0, P0 P<0, (P<0,0 <0 P<0 (P<0, < P< (P<0 (P<

Abstract Background ST-segment elevation myocardial infarction (STEMI) is one of the leading causes of fatal cardiovascular diseases, which have been the prime cause of mortality worldwide. Diagnosis in the early phase would benefit clinical intervention and prognosis, but the exploration of the biomarkers of STEMI is still lacking. Objectives In this study, we conducted a bioinformatics analysis to identify potential crucial biomarkers in the progress of STEMI. Methods We obtained GSE59867 for STEMI and stable coronary artery disease (SCAD) patients. Differentially expressed genes (DEGs) were screened with the threshold of |log2fold change| > 0.5 and p <0.05. Based on these genes, we conducted enrichment analysis to explore the potential relevance between genes and to screen hub genes. Subsequently, hub genes were analyzed to detect related miRNAs and DAVID to detect transcription factors for further analysis. Finally, GSE62646 was utilized to assess DEGs specificity, with genes demonstrating AUC results exceeding 75%, indicating their potential as candidate biomarkers. Results 133 DEGs between SCAD and STEMI were obtained. Then, the PPI network of DEGs was constructed using String and Cytoscape, and further analysis determined hub genes and 6 molecular complexes. Functional enrichment analysis of the DEGs suggests that pathways related to inflammation, metabolism, and immunity play a pivotal role in the progression from SCAD to STEMI. Besides, related-miRNAs were predicted, has-miR-124, has-miR-130a/b, and has-miR-301a/b regulated the expression of the largest number of genes. Meanwhile, Transcription factors analysis indicate that EVI1, AML1, GATA1, and PPARG are the most enriched gene. Finally, ROC curves demonstrate that MS4A3, KLRC4, KLRD1, AQP9, and CD14 exhibit both high sensitivity and specificity in predicting STEMI. Conclusions This study revealed that immunity, metabolism, and inflammation are involved in the development of STEMI derived from SCAD, and 6 genes, including MS4A3, KLRC4, KLRD1, AQP9, CD14, and CCR1, could be employed as candidate biomarkers to STEMI. STsegment ST segment (STEMI diseases worldwide prognosis lacking GSE GSE5986 (SCAD patients (DEGs log2fold logfold log fold change 05 0 5 0. 005 <0.05 Subsequently Finally GSE6264 75 75% 13 Then Cytoscape complexes metabolism Besides relatedmiRNAs predicted hasmiR124, hasmiR124 hasmiR has miR 124, 124 has-miR-124 hasmiR130a/b, hasmiR130ab hasmiRab 130a/b, 130a b has-miR-130a/b hasmiR301a/b hasmiR301ab 301a/b 301a Meanwhile EVI1 EVI AML1 AML GATA1 GATA gene MS4A3 MSA MS A KLRC4 KLRC KLRD1 KLRD AQP9 AQP CD CD1 CCR1 CCR GSE598 00 <0.0 GSE626 7 1 hasmiR12 12 has-miR-12 hasmiR130a hasmiR130a/b ab 130ab 130a/b hasmiR301a 301ab MS4A GSE59 <0. GSE62 hasmiR1 has-miR-1 hasmiRa GSE5 <0 GSE6 has-miR- < has-miR

Resumo Fundamento O infarto do miocárdio com elevação do segmento ST (IAMCSST) é uma das principais causas de doenças cardiovasculares fatais, que têm sido a principal causa de mortalidade em todo o mundo. O diagnóstico na fase inicial beneficiaria a intervenção clínica e o prognóstico, mas ainda falta a exploração dos biomarcadores do IAMCSST. Objetivos Neste estudo, conduzimos uma análise bioinformática para identificar potenciais biomarcadores cruciais no progresso do IAMCSST. Métodos Obtivemos GSE59867 para pacientes com IAMCSST e doença arterial coronariana estável (DACE). Genes diferencialmente expressos (GDEs) foram selecionados com o limiar de |log2fold change| > 0,5 e p < 0,05. Com base nesses genes, conduzimos análises de enriquecimento para explorar a relevância potencial entre genes e para rastrear genes centrais. Posteriormente, os genes centrais foram analisados para detectar miRNAs relacionados e DAVID para detectar fatores de transcrição para análise posterior. Finalmente, o GSE62646 foi utilizado para avaliar a especificidade dos GDEs, com genes demonstrando resultados de AUC superiores a 75%, indicando seu potencial como candidatos a biomarcadores. Posteriormente, os genes centrais foram analisados para detectar miRNAs relacionados e DAVID para detectar fatores de transcrição para análise posterior. Finalmente, o GSE62646 foi utilizado para avaliar a especificidade dos GDEs, com genes demonstrando resultados de AUC superiores a 75%, indicando seu potencial como candidatos a biomarcadores. Resultados 133 GDEs entre DACE e IAMCSST foram obtidos. Em seguida, a rede PPI de GDEs foi construída usando String e Cytoscape, e análises posteriores determinaram genes centrais e 6 complexos moleculares. A análise de enriquecimento funcional dos GDEs sugere que as vias relacionadas à inflamação, metabolismo e imunidade desempenham um papel fundamental na progressão de DACE para IAMCSST. Além disso, foram previstos miRNAs relacionados, has-miR-124, has-miR-130a/b e has-miR-301a/b regularam a expressão do maior número de genes. Enquanto isso, a análise dos fatores de transcrição indica que EVI1, AML1, GATA1 e PPARG são os genes mais enriquecidos. Finalmente, as curvas ROC demonstram que MS4A3, KLRC4, KLRD1, AQP9 e CD14 exibem alta sensibilidade e especificidade na previsão de IAMCSST. Conclusões Este estudo revelou que imunidade, metabolismo e inflamação estão envolvidos no desenvolvimento de IAMCSST derivado de DACE, e 6 genes, incluindo MS4A3, KLRC4, KLRD1, AQP9, CD14 e CCR1, poderiam ser empregados como candidatos a biomarcadores para IAMCSST. (IAMCSST fatais mundo prognóstico GSE GSE5986 DACE. . (DACE) (GDEs log2fold logfold log fold change 05 0 5 0, 005 0,05 Posteriormente posterior Finalmente GSE6264 75 75% 13 obtidos seguida Cytoscape moleculares disso hasmiR124, hasmiR124 hasmiR has miR 124, 124 has-miR-124 hasmiR130a/b hasmiR130ab hasmiRab 130a/b 130a b hasmiR301a/b hasmiR301ab 301a/b 301a isso EVI1 EVI AML1 AML GATA enriquecidos MS4A3 MSA MS KLRC4 KLRC KLRD1 KLRD AQP CD CD1 CCR1 CCR GSE598 (DACE 00 0,0 GSE626 7 1 hasmiR12 12 has-miR-12 hasmiR130a ab 130ab hasmiR301a 301ab MS4A GSE59 GSE62 hasmiR1 has-miR-1 hasmiRa GSE5 GSE6 has-miR- has-miR

ABSTRACT Purpose: Visual impairment and blindness caused by cataracts are major public health problems. Several factors are associated with an increased risk of age-related cataracts, such as age, smoking, alcohol consumption, and ultraviolet radiation. This meta-analysis aimed to assess the association between body mass index and age-related cataracts. Methods: Studies on weight and age-related cataracts published from January 2011 to July 2020 were reviewed by searching PubMed, Medline, and Web of Science databases. The random-effects and fixed-effects models were used for the meta-analysis, and the results were reported as odd ratios. Results: A total of nine studies were included in the meta-analysis. No correlation was found between underweight and nuclear cataracts (OR=1.31, 95% CI [-0.50 to 3.12], p=0.156). The results of the random-effects model showed that overweight was significantly associated with age-related cataracts and reduced the risk of age-related cataracts (OR=0.91, 95% CI [0.80-1.02], p<0.0001; I2=62.3%, p<0.0001). Significant correlations were found between overweight and cortical, nuclear, and posterior subcapsular cataracts (OR=0.95, 95% CI [0.66-1.24], p<0.0001; OR=0.92, 95% CI (0.76-1.08), p<0.0001; OR=0.87, 95% CI [0.38-1.02], p<0.0001). Significant correlations were found between obesity and cortical, nuclear, and posterior subcapsular cataracts (OR=1.00, 95% CI [0.82-1.17], p<0.0001; OR=1.07, 95% CI [0.92-1.22], p<0.0001; OR=1.14, 95% CI [0.91-1.37], p<0.0001). Conclusion: This finding suggested a significant correlation between body mass index and age-related cataracts, with overweight and obesity reducing or increasing the risk of age-related cataracts, respectively. Purpose problems agerelated age related smoking consumption radiation metaanalysis meta analysis Methods 201 202 PubMed Medline databases randomeffects random effects fixedeffects fixed metaanalysis, analysis, ratios Results metaanalysis. analysis. OR=1.31, OR131 OR 1 31 (OR=1.31 95 0.50 050 0 50 [-0.5 3.12, 312 3.12 , 3 12 3.12] p=0.156. p0156 p p=0.156 . 156 p=0.156) OR=0.91, OR091 91 (OR=0.91 0.801.02, 080102 0.80 1.02 80 02 [0.80-1.02] p<0.0001 p00001 0001 I2623 I I2 62 I2=62.3% p<0.0001. p<0.0001) cortical OR=0.95, OR095 (OR=0.95 0.661.24, 066124 0.66 1.24 66 24 [0.66-1.24] OR092 92 OR=0.92 0.761.08, 076108 0.76 1.08 76 08 (0.76-1.08) OR087 87 OR=0.87 0.381.02, 038102 0.38 38 [0.38-1.02] OR=1.00, OR100 00 (OR=1.00 0.821.17, 082117 0.82 1.17 82 17 [0.82-1.17] OR107 07 OR=1.07 0.921.22, 092122 0.92 1.22 22 [0.92-1.22] OR114 14 OR=1.14 0.911.37, 091137 0.91 1.37 37 [0.91-1.37] Conclusion respectively 20 OR=1.31 OR13 (OR=1.3 9 0.5 05 5 [-0. 3.1 p015 p=0.15 15 OR=0.91 OR09 (OR=0.9 801 0.801.02 08010 080 0.8 102 1.0 8 [0.80-1.02 p<0.000 p0000 000 I262 6 I2=62.3 OR=0.95 661 0.661.24 06612 066 0.6 124 1.2 2 [0.66-1.24 OR=0.9 761 0.761.08 07610 076 0.7 108 7 (0.76-1.08 OR08 OR=0.8 381 0.381.02 03810 038 0.3 [0.38-1.02 OR=1.00 OR10 (OR=1.0 821 0.821.17 08211 082 117 1.1 [0.82-1.17 OR=1.0 921 0.921.22 09212 092 0.9 122 [0.92-1.22 OR11 OR=1.1 911 0.911.37 09113 091 137 1.3 [0.91-1.37 OR=1.3 OR1 (OR=1. 0. [-0 3. p01 p=0.1 OR0 (OR=0. 0.801.0 0801 10 1. [0.80-1.0 p<0.00 p000 I26 I2=62. 0.661.2 0661 06 [0.66-1.2 OR=0. 0.761.0 0761 (0.76-1.0 0.381.0 0381 03 [0.38-1.0 0.821.1 0821 11 [0.82-1.1 OR=1. 0.921.2 0921 09 [0.92-1.2 0.911.3 0911 13 [0.91-1.3 (OR=1 [- p0 p=0. (OR=0 0.801. [0.80-1. p<0.0 p00 I2=62 0.661. [0.66-1. OR=0 0.761. (0.76-1. 0.381. [0.38-1. 0.821. [0.82-1. OR=1 0.921. [0.92-1. 0.911. [0.91-1. (OR= [ p=0 0.801 [0.80-1 p<0. I2=6 0.661 [0.66-1 OR= 0.761 (0.76-1 0.381 [0.38-1 0.821 [0.82-1 0.921 [0.92-1 0.911 [0.91-1 (OR p= [0.80- p<0 I2= [0.66- (0.76- [0.38- [0.82- [0.92- [0.91- [0.80 p< [0.66 (0.76 [0.38 [0.82 [0.92 [0.91 [0.8 [0.6 (0.7 [0.3 [0.9 [0. (0. [0 (0 (

RESUMO Objetivo: A deficiência visual e a cegueira causadas pela catarata são um grande problema de saúde pública. Há vários fatores associados a um risco maior de catarata relacionada à idade na população mundial, tais como idade, tabagismo, consumo de álcool e radiação ultravioleta. Esta meta-análise foi realizada para avaliar a associação entre o índice de massa corporal e a catarata relacionada à idade. Métodos: Foi revisada a literatura sobre catarata relacionada a peso e idade publicada de janeiro de 2011 a julho de 2020, através de buscas nos bancos de dados PubMed, Medline e Web of Science. Na meta-análise, foram utilizados modelos de efeito aleatórios e de efeitos fixos e os resultados foram apresentados como razões de chances (OR). Resultados: Um total de 9 estudos foi incluído na meta-análise. Não houve correlação entre ausência de sobrepeso e cataratas nucleares (OR=1,31, IC 95%: -0,50-3,12, p=0,156). Os resultados do modelo de efeitos aleatórios mostraram que o excesso de peso estava significativamente associado a uma redução do risco de catarata relacionada à idade (OR=0,91, IC 95%: 0,80-1,02, p<0,0001, I2=62,3%, p<0,0001). Houve correlações significativas entre o excesso de peso e cataratas corticais (OR=0,95, IC 95%: 0,66-1,24, p<0,0001), nucleares (OR=0,92, IC 95%: 0,76-1,08, p<0,0001) e subcapsulares posteriores (OR=0,87, IC 95%: 0,38-1,02, p<0,0001) relacionadas à idade. Houve correlações significativas entre obesidade e cataratas corticais (OR=1,00, IC 95%: 0,82-1,17, p<0,0001), nucleares (OR=1,07, IC 95%: 0,92-1,22, p<0,0001) e subcapsulares posteriores (OR=1,14, IC 95%: 0,91-1,37, p<0,0001) relacionadas à idade. Conclusão: Estes achados sugeriram uma correlação significativa entre o índice de massa corporal e a catarata relacionada à idade, com o excesso de peso e a obesidade reduzindo e aumentando o risco de catarata relacionada à idade, respectivamente. Objetivo pública mundial tabagismo ultravioleta metaanálise meta análise Métodos 201 2020 PubMed Science metaanálise, análise, OR. OR . (OR) Resultados metaanálise. análise. OR=1,31, OR131 1 31 (OR=1,31 95% 95 0,503,12, 050312 0,50 3,12, 0 50 3 12 -0,50-3,12 p=0,156. p0156 p p=0,156 156 p=0,156) OR=0,91, OR091 91 (OR=0,91 0,801,02, 080102 0,80 1,02, 80 02 0,80-1,02 p00001 0001 p<0,0001 I2623 I I2 62 I2=62,3% p<0,0001. OR=0,95, OR095 (OR=0,95 0,661,24, 066124 0,66 1,24, 66 24 0,66-1,24 , OR=0,92, OR092 92 (OR=0,92 0,761,08, 076108 0,76 1,08, 76 08 0,76-1,08 OR=0,87, OR087 87 (OR=0,87 0,381,02, 038102 0,38 38 0,38-1,02 OR=1,00, OR100 00 (OR=1,00 0,821,17, 082117 0,82 1,17, 82 17 0,82-1,17 OR=1,07, OR107 07 (OR=1,07 0,921,22, 092122 0,92 1,22, 22 0,92-1,22 OR=1,14, OR114 14 (OR=1,14 0,911,37, 091137 0,91 1,37, 37 0,91-1,37 Conclusão respectivamente 20 202 (OR OR=1,31 OR13 (OR=1,3 503 0,503,12 05031 050 0,5 312 3,12 5 -0,50-3,1 p015 p=0,15 15 OR=0,91 OR09 (OR=0,9 801 0,801,02 08010 080 0,8 102 1,02 8 0,80-1,0 p0000 000 p<0,000 I262 6 I2=62,3 OR=0,95 661 0,661,24 06612 066 0,6 124 1,24 2 0,66-1,2 OR=0,92 761 0,761,08 07610 076 0,7 108 1,08 7 0,76-1,0 OR=0,87 OR08 (OR=0,8 381 0,381,02 03810 038 0,3 0,38-1,0 OR=1,00 OR10 (OR=1,0 821 0,821,17 08211 082 117 1,17 0,82-1,1 OR=1,07 921 0,921,22 09212 092 0,9 122 1,22 0,92-1,2 OR=1,14 OR11 (OR=1,1 911 0,911,37 09113 091 137 1,37 0,91-1,3 OR=1,3 OR1 (OR=1, 0,503,1 0503 05 0, 3,1 -0,50-3, p01 p=0,1 OR=0,9 OR0 (OR=0, 0,801,0 0801 10 1,0 0,80-1, p000 p<0,00 I26 I2=62, 0,661,2 0661 06 1,2 0,66-1, 0,761,0 0761 0,76-1, OR=0,8 0,381,0 0381 03 0,38-1, OR=1,0 0,821,1 0821 11 1,1 0,82-1, 0,921,2 0921 09 0,92-1, OR=1,1 0,911,3 0911 13 1,3 0,91-1, OR=1, (OR=1 0,503, 3, -0,50-3 p0 p=0, OR=0, (OR=0 0,801, 1, 0,80-1 p00 p<0,0 I2=62 0,661, 0,66-1 0,761, 0,76-1 0,381, 0,38-1 0,821, 0,82-1 0,921, 0,92-1 0,911, 0,91-1 OR=1 (OR= 0,503 -0,50- p=0 OR=0 0,801 0,80- p<0, I2=6 0,661 0,66- 0,761 0,76- 0,381 0,38- 0,821 0,82- 0,921 0,92- 0,911 0,91- OR= -0,50 p= p<0 I2= -0,5 p< -0, -0 -

ABSTRACT: Malus is an important ornamental plant around the world and widely used in commercial production due to its diversity and wide adaptability. ‘Zi Dieer’ crabapple has good adaptability to a wide temperature range (high and low), disease resistance, and high commercial value. To satisfy the market demand, we established ‘Zi Dieer’ crabapple micro-regeneration system for large-scale reproduction. Induced buds were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP: 0.5, 1.0, 1.5 mg/L) and 1-naphthaleneacetic acid (NAA: 0.05, 0.1, 0.3 mg/L) for 35 days. The highest bud induction rate (93.3%) was observed on MS medium supplemented with 1.0 mg/L BAP and 0.1 mg/L NAA, with average micro-shoot length of 1.32 cm. The germinated buds (1 cm in length) were propagated and cultured on MS medium supplemented with BAP (1.0, 1.5, 2.0 mg/L) and NAA (0.1, 0.3, 0.5 mg/L), after 35 days, on the MS basal medium supplemented with 1.5 mg/L BAP and 0.3 mg/L NAA, the best average number of new shoots is 8.56±0.13 (P<0.05) and good shoot length (4.94±0.09 cm), reproduction coefficient higher than MS medium (0) without PGRs. In order to induce rooting, the shoots (length 2 cm) are inserted in half strength MS (1/2 MS) medium containing 0.1, 0.3 and 0.5 mg/L of 3-indole butyric acid (IBA) or NAA, and 1 g/L activated carbon (AC) was added on the basis of NAA 0.3 mg/L to study the effect of activated carbon on the rooting of crabapple. After 38 days of culture, compared with other treatments, among the explants treated with IBA, the rooting rate was higher in the 1/2 MS medium containing 0.1 mg/L (82.1%), and the highest root number (11.29±0.52) was observed, and the root length was the other treatments 2-3 times. Although, the highest rooting rate was observed on 1/2 MS medium containing 0.1mg/L NAA (87.8 %), but the bottom of these buds formed large callus, which was not conducive to survival, and the root length was short. In summary, a feasible and complete rapid propagation program for ‘Zi Dieer’ crabapple has been developed. ABSTRACT Zi Dieer low, low , low) resistance value demand microregeneration micro regeneration largescale scale (MS 6benzylaminopurine benzylaminopurine 6 (BAP 05 0 5 10 15 1. mgL mg L 1naphthaleneacetic naphthaleneacetic (NAA 005 0.05 01 03 3 0. 93.3% 933 93 (93.3% microshoot 132 32 1.3 ( (1.0 20 2. (0.1 mg/L, 856013 8 56 13 8.56±0.1 P<0.05 P005 P (P<0.05 4.94±0.09 494009 4 94 09 (4.94±0.0 cm, (0 PGRs 12 (1/ 3indole indole IBA (IBA gL g AC (AC culture 1/ 82.1%, 821 82.1% 82 (82.1%) 11.29±0.52 1129052 11 29 52 (11.29±0.52 23 2- times Although 01mgL 1mg 87.8 878 87 (87. %, % %) callus survival short summary developed 00 0.0 93.3 9 (93.3 (1. (0. 85601 8.56±0. P<0.0 P00 (P<0.0 4.94±0.0 49400 (4.94±0. 82.1 (82.1% 11.29±0.5 112905 (11.29±0.5 87. (87 93. (93. 8560 8.56±0 P<0. P0 (P<0. 4.94±0. 4940 (4.94±0 82. (82.1 11.29±0. 11290 (11.29±0. (8 (93 856 8.56± P<0 (P<0 4.94±0 494 (4.94± (82. 11.29±0 1129 (11.29±0 (9 85 8.56 P< (P< 4.94± 49 (4.94 (82 11.29± 112 (11.29± 8.5 (P 4.94 (4.9 11.29 (11.29 8. 4.9 (4. 11.2 (11.2 4. (4 11. (11. (11

RESUMO: Malus é uma planta ornamental importante no mundo e muito utilizada na produção comercial devido à sua diversidade e ampla adaptabilidade. A maçã silvestre ‘Zi Dieer’ apresentou boa adaptabilidade a altas e baixas temperaturas, resistência a doenças e maior valor comercial. Para satisfazer a demanda do mercado, estabelecemos o sistema de microrregeneração de maçã silvestre ‘Zi Dieer’ para reprodução em larga escala. Os botões induzidos foram cultivados em meio Murashige e Skoog (MS) suplementado com 6-benzilaminopurina (BAP) 0,5, 1,0, 1,5 mg/L e ácido 1-naftalenoacético (NAA) 0,05, 0,1, 0,3 mg/L por 35 dias. A maior taxa de indução de gemas foi em meio MS suplementado com 1,0 mg/L de BAP e 0,1 mg/L de NAA, que foi de 93,3% e o comprimento médio dos microbrotos foi de 1,32 cm. Os botões germinados (comprimento 1 cm) foram propagados e cultivados em meio MS suplementado com BAP e NAA de 1,0, 1,5, 2,0 e 0,1, 0,3, 0,5 mg/L, após 35 dias, em meio MS basal suplementado com 1,5 mg/L. L BAP e 0,3 mg/L ANA, o melhor número médio de brotos novos é 8,56±0,13 (P<0,05) e bom comprimento de brotos (4,94±0,09 cm), coeficiente de reprodução superior ao meio MS (0) sem PGRs. Para induzir o enraizamento, os brotos (comprimento 2 cm) são inseridos em meio MS de meia concentração (1/2 MS) contendo 0,1, 0,3 e 0,5 mg/L de ácido 3-indol butírico (IBA) ou ANA, e 1 g /L carvão ativado (CA) foi adicionado com base em ANA 0,3 mg/L para estudar o efeito do carvão ativado no enraizamento da maçã silvestre. Duas semanas depois, observando a geração de raízes. Após 38 dias de cultivo, comparado aos demais tratamentos, entre os explantes tratados com AIB, a taxa de enraizamento foi maior no meio 1/2 MS contendo 0,1 mg/L (82,1%) e o maior número de raízes (11,29±0,52) foi observado, e o comprimento da raiz foi igual aos demais tratamentos 2 a 3 vezes. Embora a maior taxa de enraizamento tenha sido observada em meio MS 1/2 contendo 0,1mg/L de NAA (87,8%), mas a parte inferior dessas gemas forma um grande calo, que não é propício à sobrevivência, e o comprimento da raiz é curto. Em resumo, foi desenvolvido um programa de propagação rápida viável e completo para a maçã silvestre ‘Zi Dieer’. RESUMO Zi Dieer temperaturas mercado escala (MS 6benzilaminopurina benzilaminopurina 6 (BAP 05 0 5 10 15 1, mgL mg 1naftalenoacético naftalenoacético (NAA 005 0,05 01 03 0, 933 93 93,3 132 32 1,3 cm 20 2, 856013 8 56 13 8,56±0,1 P<0,05 P005 P (P<0,05 4,94±0,09 494009 4 94 09 (4,94±0,0 cm, , (0 PGRs 12 (1/ 3indol indol IBA (IBA CA (CA depois cultivo AIB 1/ 82,1% 821 82 (82,1% 11,29±0,52 1129052 11 29 52 (11,29±0,52 observado vezes 01mgL 1mg 87,8%, 878 87,8% 87 (87,8%) calo sobrevivência curto resumo Dieer. . 00 0,0 9 93, 85601 8,56±0, P<0,0 P00 (P<0,0 4,94±0,0 49400 (4,94±0, ( (1 82,1 (82,1 11,29±0,5 112905 (11,29±0,5 87,8 (87,8% 8560 8,56±0 P<0, P0 (P<0, 4,94±0, 4940 (4,94±0 82, (82, 11,29±0, 11290 (11,29±0, 87, (87,8 856 8,56± P<0 (P<0 4,94±0 494 (4,94± (82 11,29±0 1129 (11,29±0 (87, 85 8,56 P< (P< 4,94± 49 (4,94 (8 11,29± 112 (11,29± (87 8,5 (P 4,94 (4,9 11,29 (11,29 8, 4,9 (4, 11,2 (11,2 4, (4 11, (11, (11

Adenine nucleotide translocator 4 (Ant4), an ATP/ADP transporter expressed in the early phases of spermatogenesis, plays a crucial role in male fertility. While Ant4 loss causes early arrest of meiosis and increased apoptosis of spermatogenic cells in male mice, its other potential functions in male fertility remain unexplored. Here, we utilized Ant4 knockout mice to delineate the effects of Ant4-deficiency on male reproduction. Our observations demonstrated that Ant4-deficiency led to infertility and impaired testicular development, which was further investigated by evaluating testicular oxidative stress, autophagy, and inflammation. Specifically, the loss of Ant4 led to an imbalance of oxidation and antioxidants. Significant ultrastructural alterations were identified in the testicular tissues of Ant4-deficient mice, including swelling of mitochondria, loss of cristae, and accumulation of autophagosomes. Our results also showed that autophagic flux and AKT-AMPK-mTOR signaling pathway were affected in Ant4-deficient mice. Moreover, Ant4 loss increased the expression of pro-inflammatory factors. Overall, our findings underscored the importance of Ant4 in regulating oxidative stress, autophagy, and inflammation in testicular tissues. Taken together, these insights provided a nuanced understanding of the significance of Ant4 in testicular development. Ant4, Ant , (Ant4) ATPADP ATP ADP spermatogenesis unexplored Here Ant4deficiency Antdeficiency deficiency reproduction development stress autophagy Specifically antioxidants Ant4deficient Antdeficient deficient mitochondria cristae autophagosomes AKTAMPKmTOR AKT AMPK mTOR Moreover proinflammatory pro inflammatory factors Overall together (Ant4 (Ant

Abstract Background Dermatomyositis (DM) is an infrequent disease subgroup of idiopathic inflammatory myopathies characterized by distinct skin lesions. However, high heterogeneity makes clinical diagnosis and treatment of DM very challenging. Objectives Unsupervised classification in DM patients and analysis of key factors related to clinical outcomes. Methods This retrospective study was conducted between 2017 and 2022 at the Department of Rheumatology, Xiangya Hospital, Central South University. 162 DM patients were enrolled for unsupervised hierarchical cluster analysis. In addition, we divided the clinical outcomes of DM patients into four subgroups: withdrawal, stabilization, aggravation, and death, and compared the clinical profiles amongst the subgroups. Results Out of 162 DM patients, three clusters were defined. Cluster 1 (n = 40) was mainly grouped by patients with prominent muscular involvement and mild Interstitial Lung Disease (ILD). Cluster 2 (n = 72) grouped patients with skin rash, anti-Melanoma Differentiation Associated protein 5 positive (anti-MDA5+), and Rapid Progressive Interstitial Lung Disease (RP-ILD). Cluster 3 (n = 50) grouped patients with the mildest symptoms. The proportion of death increased across the three clusters (cluster 3 < cluster 1 < cluster 2). Study limitations The number of cases was limited for the subsequent construction and validation of predictive models. We did not review all skin symptoms or pathological changes in detail. Conclusions We reclassified DM into three clusters with different risks for poor outcome based on diverse clinical profiles. Clinical serological testing and cluster analysis are necessary to help clinicians evaluate patients during follow-up and conduct phenotype-based personalized care in DM. (DM lesions However challenging 201 202 Rheumatology Hospital University 16 addition subgroups withdrawal stabilization aggravation defined n 40 ILD. ILD . (ILD) 72 rash antiMelanoma anti Melanoma antiMDA5+, antiMDA5 antiMDA MDA5+ , MDA5 MDA (anti-MDA5+) RPILD. RPILD RP (RP-ILD) 50 2. 2) models detail followup follow up phenotypebased phenotype 20 4 (ILD 7 antiMDA5+ (anti-MDA5+ (RP-ILD (anti-MDA5 (anti-MDA

Abstract Objectives The prognosis of patients with Small Cell Lung Cancer (SCLC) can be predicted by their Lymph Node (LN) status. The authors aimed to assess the correlations between SCLC survival and number of LN Ratio (LNR), positive LN (pLNs), and Logarithmic Odds of positive LN (LODDS). Methods This cohort study retrospectively included 1,762 patients with SCLC from the SEER database 2004‒2015. The X-tile software was used to determine the cutoff values for pLNs, LNR, and LODDS. The correlations between pLNs, LNR, and LODDS with Overall Survival (OS) and Cancer-Specific Survival (CSS) were explored using Cox regression analysis. The study used the C-index to assess the predictive value of LNR, pLNs, and LODDS on survival. Results Among these 1,762 patients, 121 (6.87%) were alive, 1,641 (93.13%) died, and 1,532 (86.95%) died of SCLC. In univariable COX analysis, LNR, pLNs, and LODDS all showed a correlation with CSS and OS (p < 0.05). In multivariable COX analysis, only patients with LODDS (> 0.3 vs. ≤ 0.3) were related to both worse OS (HR = 1.28, 95% CI 1.10‒1.50) and CSS (HR = 1.29, 95% CI 1.10‒1.51), but no correction was observed between LNR and pLNs and survival (p > 0.05). The C-indices for predicting OS for LODDS were 0.552 (95% CI 0.541‒0.563), for LNR 0.504 (95% CI 0.501‒0.507), and for pLNs 0.527 (95% CI 0.514‒0.540). Moreover, the association between LODDS and prognosis in SCLC patients was significant only in patients with LN stage N1 and N2, but not in stage N3. Conclusion LODDS may be better than other LN assessment tools at predicting survival in SCLC patients. (SCLC (LN status , (LNR) (pLNs) . (LODDS) 1762 1 762 1,76 20042015 2004 2015 2004‒2015 Xtile X tile (OS CancerSpecific Specific (CSS analysis Cindex C index 12 6.87% 687 6 87 (6.87% alive 1641 641 1,64 93.13% 9313 93 13 (93.13% 1532 532 1,53 86.95% 8695 86 95 (86.95% p 0.05. 005 0.05 0 05 0.05) ( 03 3 0. vs HR 128 28 1.28 1.10‒1.50 110150 10 50 129 29 1.29 1.10‒1.51, 110151 1.10‒1.51 51 1.10‒1.51) Cindices indices 0552 552 0.55 (95 0.541‒0.563, 05410563 0.541‒0.563 541 563 0.541‒0.563) 0504 504 0.50 0.501‒0.507, 05010507 0.501‒0.507 501 507 0.501‒0.507) 0527 527 0.52 0.514‒0.540. 05140540 0.514‒0.540 514 540 0.514‒0.540) Moreover N N2 N3 (LNR (pLNs (LODDS 176 76 1,7 2004201 200 201 2004‒201 6.87 68 8 (6.87 164 64 1,6 93.13 931 9 (93.13 153 53 1,5 86.95 869 (86.95 00 0.0 2 1.2 1.10‒1.5 11015 5 055 55 0.5 (9 0541056 0.541‒0.56 54 56 050 0501050 0.501‒0.50 052 52 0514054 0.514‒0.54 17 7 1, 200420 20 2004‒20 6.8 (6.8 16 93.1 (93.1 15 86.9 (86.9 1. 1.10‒1. 1101 054105 0.541‒0.5 050105 0.501‒0.5 051405 0.514‒0.5 20042 2004‒2 6. (6. 93. (93. 86. (86. 1.10‒1 110 05410 0.541‒0. 05010 0.501‒0. 05140 0.514‒0. 2004‒ (6 (93 (86 1.10‒ 11 0541 0.541‒0 0501 0.501‒0 0514 0.514‒0 (8 1.10 054 0.541‒ 0.501‒ 051 0.514‒ 1.1 0.541 0.501 0.514 0.54 0.51

RESUMO: Neste estudo, compararmos a fenologia da floração, os açúcares solúveis e as proteínas solúveis do pólen, as enzimas antioxidantes e as características de armazenamento de cinco populações cultivadas de Paeonia ostii ‘Feng dan’ em diferentes altitudes em Luoyang, na perspectiva da biologia da polinização. O pólen é um fruto de uma espécie de árvore, com um teor de proteínas solúveis e de pólen, sistema de enzimas antioxidantes e características de armazenamento. Os resultados mostraram que o início da floração foi mais tardio em altitudes mais elevadas. O teor de açúcar solúvel do pólen variou significativamente entre diferentes locais, não havendo uma relação óbvia com a altitude, enquanto o teor de proteínas solúveis tendeu a diminuir com o aumento da altitude. As atividades das enzimas antioxidantes do pólen de populações cultivadas a diferentes altitudes diferiram entre si, as atividades das enzimas SOD e POD aumentaram basicamente com a diminuição da temperatura, enquanto a enzima CAT apresentou várias tendências. Verificaram-se diferenças no tempo de armazenamento do pólen de populações de diferentes altitudes, e o pólen de populações de altitudes mais elevadas parecia estar mais bem adaptado a ambientes de armazenamento a baixas temperaturas. A taxa de redução da viabilidade do pólen sob armazenamento seco a -74,7 °C foi significativamente menor do que sob outras condições de armazenamento. As análises sugerem que as diferenças entre as populações de cultivo de ‘Fengdan’ na mesma região a diferentes altitudes podem ser exploradas para efetuar a polinização cruzada entre populações próximas, utilizando a tecnologia de armazenamento de pólen por secagem a baixa temperatura, com vista a atingir o objetivo de produção abundante e em grande escala de peónia oleaginosa. RESUMO estudo Feng dan Luoyang árvore locais altitude si temperatura tendências Verificaramse Verificaram se temperaturas 74,7 747 74 7 -74, C ‘Fengdan Fengdan próximas oleaginosa 74, -74 -7 -

ABSTRACT: From a pollination biology standpoint, this study compared modifications in the flowering phenology, soluble sugar and protein content of pollen, antioxidant enzyme system, and storage traits of five cultivated populations of Paeonia ostii ‘Feng dan’ at various altitudes in Luoyang. The findings indicated that the first blooming phase was delayed at greater altitudes. There was a significant difference in the soluble sugar content of pollen between sites; and there was no discernible relationship between soluble sugar content and altitude. With increasing altitude, there is a tendency for soluble protein content to decrease. The activities of antioxidant enzymes in pollen from cultivated populations at varying altitudes showed SOD and POD enzyme activities generally increasing with decreasing temperature, whereas CAT enzymes showed divergent patterns. Differences existed in the storage time of pollen across populations at different altitudes, and pollen from higher altitudes appeared to be better adapted to low-temperature storage environments. The rate of decrease in pollen viability under dry storage at -74.7 °C was significantly lower than under other storage conditions. The comprehensive analysis concludes that the variations in ‘Feng dan’ cultivation populations within the same region at different altitude zones can be leveraged for cross-pollination among proximate populations, utilizing low-temperature dry pollen storage technology, with the aim of achieving abundant oil peony production. ABSTRACT standpoint phenology system Feng dan Luoyang sites temperature patterns lowtemperature low environments 74.7 747 74 7 -74. C conditions crosspollination cross technology production 74. -74 -7 -

Abstract Objective To analyze the effect of mast cells (MCs) in neurogenic inflammation and the neuroimmune response of trigeminal ganglia (TG) due to pulpitis and detect the regulatory effect of curcumin (Cur) on neuroimmune responses induced by pulpitis. Methodology Immunohistochemistry, toluidine blue staining (TB), and other methods were used to detect the dynamic changes of MCs, as well as tryptase expression changes and protease activated receptor 2 (PAR2) and calcitonin gene-related peptide (CGRP) levels in the neuroimmune response induced by pulpitis. After administering Cur by intraperitoneal injection, the expression levels of Toll-like receptor 4 (TLR4), CGRP, glial fibrillary acidic protein (GFAP), fractalkine (CX3CL1), Tumor necrosis factor (TNF-α), and other factors were examined in the TG of pulpitis-induced rats. Results After pulpitis induction, the expression of CGRP-positive neurons and GFAP-positive soluble guanylate cyclase (SGC) in the TG significantly increased. A large number of MCs underwent degranulation. MCs were scattered between the CGRP-positive nerve fibers. MCs showing a typical degranulated state within the TG significantly increased and tryptase-positive MCs surrounded the TG nerve fibers and neurons. After treatment with Cur, the inflammatory response in the periodontal bone induced by pulpitis decreased and promoted early tissue repair. The expression of TNF-α significantly decreased as did degranulation of MCs. In contrast, the expression of CGRP, TLR4-positive neurons, activated SGCs, and PAR2-positive TG neurons significantly decreased. MCs could participate in the neuroimmune response induced by pulpitis by the tryptase signaling pathway. Conclusion Importantly, Cur inhibited the degranulation of MCs, downregulated the expression of tryptase and PAR2 in the TG, and attenuated the activation response of osteoclasts in the apical periodontium. (MCs (TG (Cur Immunohistochemistry TB, TB , (TB) PAR (PAR2 generelated gene related CGRP (CGRP injection Tolllike Toll like TLR4, TLR4 TLR (TLR4) GFAP, GFAP (GFAP) CX3CL1, CX3CL1 CXCL CX CL (CX3CL1) TNFα, TNFα TNF α (TNF-α) pulpitisinduced rats induction CGRPpositive positive GFAPpositive SGC (SGC tryptasepositive repair contrast TLR4positive TLRpositive SGCs PAR2positive PARpositive pathway Importantly periodontium (TB (PAR (TLR4 (GFAP CX3CL (CX3CL1 (TNF-α (TLR (CX3CL

Abstract Introduction: dysphagia is a common complication of stroke, and serum albumin is widely recognized as a strong prognostic marker of health and/or disease status. However, the correlation between dysphagia and serum albumin levels has not been established. Objectives: to observe the correlation between dysphagia and serum albumin levels and prognosis in patients with stroke. Methods: we performed a retrospective study of patients hospitalized between June 1, 2018, and June 1, 2022. A total of 1,370 patients were enrolled. The patients were divided into two groups: dysphagia and non-dysphagia. Binary logistic regression and multiple linear regression models were used to analyze the correlation between dysphagia, albumin, modified Rankin Scale (mRS), activities of daily living (ADL), and length of hospital stay (LOS). Results: after adjusting for confounding factors, the risk of pneumonia in the dysphagia group was 2.417 times higher than that in the non-dysphagia group (OR = 2.417, 95 % CI: 1.902-3.072, p = 0.000). The risk of mRS ≥ 3 and modified Barthel index (MBI) < 60 in patients with dysphagia was 3.272-fold (OR = 3.272, 95 % CI: 2.508-4.269, p < 0.001) and 1.670-fold (OR = 1.670, 95 % CI: 1.230-2.268, p < 0.001), respectively; and the risk of hypoproteinemia was 2.533 times higher (OR = 2.533, 95 % CI: 1.879-3.414, p = 0.000). Stepwise linear regression showed that dysphagia was significantly correlated with lower albumin levels and higher mRS, lower ADL, and longer LOS in patients with stroke (β = -0.220, β = 0.265, β = -0.210, and β = 0.147, respectively; p < 0.001). Conclusions: dysphagia in patients with stroke is associated with decreased albumin levels and has an impact on its prognosis.

Resumen Introducción: la disfagia es una complicación común del accidente cerebrovascular, y la albúmina sérica es ampliamente reconocida como un fuerte marcador pronóstico del estado de salud y/o enfermedad. Sin embargo, no se ha establecido la correlación entre la disfagia y los niveles de albúmina sérica. Objetivos: observar la correlación entre la disfagia y los niveles de albúmina sérica y el pronóstico en pacientes con accidente cerebrovascular. Métodos: realizamos un estudio retrospectivo de pacientes hospitalizados entre el 1 de junio de 2018 y el 1 de junio de 2022. Se inscribieron un total de 1.370 pacientes, los cuales fueron divididos en dos grupos: con disfagia y sin disfagia. Se utilizaron modelos de regresión logística binaria y de regresión lineal múltiple para analizar la correlación entre la disfagia, la albúmina, la escala de Rankin modificada (ERm), las actividades de la vida diaria (AVD) y el tiempo de estancia hospitalaria (TEH). Resultados: después de ajustar por factores de confusión, el riesgo de neumonía en el grupo de disfagia fue 2,417 veces mayor que en el grupo sin disfagia (OR = 2,417, IC 95 %: 1,902-3,072, p = 0,000). El riesgo de ERm ≥ 3 y el índice de Barthel modificado (MBI) < 60 en pacientes con disfagia se multiplicó por 3,272 veces (OR = 3,272, IC 95 %: 2,508-4,269, p < 0,001) y 1,670 veces (OR = 1,670, IC 95 %: 1,230-2,268, p < 0,001), respectivamente; el riesgo de hipoproteinemia fue 2,533 veces mayor (OR = 2,533, IC 95 %: 1,879-3,414, p = 0,000). La regresión lineal por pasos mostró que la disfagia se correlacionó significativamente con niveles más bajos de albúmina y ERm más altos, AVD más bajos y TEH más prolongados en pacientes con accidente cerebrovascular (β = -0,220, β = 0,265, β = -0,210 y β = 0,147, respectivamente; p < 0,001). Conclusiones: la disfagia en pacientes con accidente cerebrovascular se asocia a una disminución de los niveles de albúmina y repercute en su pronóstico.

ABSTRACT Finger citron (Citrus medica L. var. sarcodactylis (Hoola van Nooten) Swingle; Rutaceae: Citrus) is an important plant for both medicine and food. Due to the lack of suitability analysis, many problems have arisen in its planting. According to the daily observation data, Kriging interpolation was selected to spatialize precipitation and temperature data. MaxEnt and ArcGIS were applied to simulate the suitable areas of finger citron in China from the perspectives of bioclimate, soil, topographic factors and human activities in 2050s and 2090s. Results showed that temperature annual range (Bio7), annual precipitation (Bio12), human footprint (Hf), elevation (El) and precipitation seasonality (Bio15) were identified as the dominant environmental variables related to the distribution of finger citron. Spatiotemporal distribution of annual precipitation (Bio12) showed that in the future, the precipitation in South China tends to decrease first (2050s) and then increase (2090s). The spatio-temporal analysis of temperature annual range (Bio7) showed that the 20-30 ℃ region was relatively stable in the Sichuan Basin and the middle and lower reaches of the Pearl River Basin. Under the future climate change scenarios, the total suitable area showed a significant increase trend in 2090s, and the change of most, moderately and poorly suitable habitats showed no obvious law. Under SSP1-2.6, SSP2-4.5 and SSP5-8.5 scenarios, the centroid of the most suitable habitat of finger citron would move to the northwest, southeast and southwest respectively. Our results can effectively carry out to promote the recovery of its population.

Resumen Objetivo: Investigar los efectos de la intervención basada en el modelo de creencias de salud en los comportamientos de las enfermeras en términos de mantener húmedos los instrumentos quirúrgicos. Método: Se realizaron encuestas previas y posteriores a la capacitación sobre la humectación de instrumentos con las mismas 356 enfermeras de un hospital en China. Ambas encuestas contenían un cuestionario sobre el conocimiento general relacionado con la humectación del instrumento, preguntas basadas en escalas de percepción sobre el mismo tema y un formulario de inspección sobre la implementación de los procedimientos de humectación. Se proporcionó una formación de 3 meses a las enfermeras. Resultados: Después de la capacitación se mejoraron los conocimientos, las actitudes, las creencias y los comportamientos de las enfermeras para la humectación del instrumento. Conclusiones: La intervención basada en el modelo de creencias en salud puede generar una mejora significativa en el cumplimiento de los requisitos de humectación del instrumental quirúrgico por parte de las enfermeras, y las correspondientes mejoras en la limpieza del instrumental y la seguridad del paciente.

Abstract Objective: We investigated the effects of the Health Belief Model (HBM)-based intervention on nurses' behaviors in terms of keeping surgical instruments moist. Materials and methods: Pre- and post-training surveys about instrument moistening were conducted with the same 356 nurses from a hospital in China. Both of the surveys contained questionnaire concerning general knowledge relating to instrument moistening, perception scale-based questions concerning the same issue, and an inspection form concerning the implementation of moistening procedures. Three months' training was provided for the nurses. Results: After training, the nurses' knowledge, attitudes, beliefs, and behaviors for instrument moistening were improved. Conclusions: The HBM-based intervention can bring about a significant improvement in nurses' compliance with surgical instrument moistening requirements, and corresponding improvements in instrument cleanliness and patient safety.