ABSTRACT BACKGROUND: Red blood cell distribution width (RDW) is related to sepsis-related mortality. Hemophagocytic lymphohistiocytosis (HLH) is a syndrome caused by severe infection, tumors, or autoimmunity without a specific diagnosis. OBJECTIVE: To explore the correlation between RDW and mortality in patients with HLH. DESIGN AND SETTING: A retrospective study conducted in a hospital in China. METHODS: A total of 101 inpatients with HLH from January 1, 2017 to December 31, 2021 were divided into non-survivor (n = 52) and survivor (n = 49) groups. A non-parametric test was used to analyze demographic, clinical, and laboratory data between groups. Independent variables with P < 0.05 were analyzed using binary logistic regression to screen out mortality-related variables. Selected variables were subjected to multivariate logistic regression analysis, and those with strong correlations were screened. Receiver operating characteristic (ROC) curves of strongly correlated variables and area under curve (AUC) values were obtained. RESULTS: The APACHE II score, RDW, and platelet (PLT) and fibrinogen (FIB) levels (P < 0.05) different significantly. RDW, PLT, FIB were correlated with mortality. The AUC values of RDW, PLT, and FIB were 0.857, 0.797, and 0.726, respectively. RDW was associated with mortality in patients with HLH (P < 0.01, cut-off value: 16.9). The sensitivity and specificity of predicting mortality were 97.96% and 96.1%, respectively. CONCLUSION: Logistic regression analysis showed a correlation between RDW and patients’ mortality. Therefore, RDW can be used to predict mortality in patients with HLH.

ABSTRACT BACKGROUND: Red blood cell distribution width (RDW) is related to sepsis-related mortality. Hemophagocytic lymphohistiocytosis (HLH) is a syndrome caused by severe infection, tumors, or autoimmunity without a specific diagnosis. OBJECTIVE: To explore the correlation between RDW and mortality in patients with HLH. DESIGN AND SETTING: A retrospective study conducted in a hospital in China. METHODS: A total of 101 inpatients with HLH from January 1, 2017 to December 31, 2021 were divided into non-survivor (n = 52) and survivor (n = 49) groups. A non-parametric test was used to analyze demographic, clinical, and laboratory data between groups. Independent variables with P < 0.05 were analyzed using binary logistic regression to screen out mortality-related variables. Selected variables were subjected to multivariate logistic regression analysis, and those with strong correlations were screened. Receiver operating characteristic (ROC) curves of strongly correlated variables and area under curve (AUC) values were obtained. RESULTS: The APACHE II score, RDW, and platelet (PLT) and fibrinogen (FIB) levels (P < 0.05) different significantly. RDW, PLT, FIB were correlated with mortality. The AUC values of RDW, PLT, and FIB were 0.857, 0.797, and 0.726, respectively. RDW was associated with mortality in patients with HLH (P < 0.01, cut-off value: 16.9). The sensitivity and specificity of predicting mortality were 97.96% and 96.1%, respectively. CONCLUSION: Logistic regression analysis showed a correlation between RDW and patients’ mortality. Therefore, RDW can be used to predict mortality in patients with HLH. BACKGROUND (RDW sepsisrelated sepsis (HLH infection tumors diagnosis OBJECTIVE SETTING China METHODS 10 1 201 31 202 nonsurvivor non n 52 49 groups nonparametric parametric demographic clinical 005 0 05 0.0 mortalityrelated screened ROC (ROC (AUC obtained RESULTS score PLT (PLT (FIB significantly 0857 857 0.857 0797 797 0.797 0726 726 0.726 respectively 001 01 0.01 cutoff cut off value 16.9. 169 16.9 . 16 9 16.9) 9796 97 96 97.96 961 96.1% CONCLUSION Therefore 20 3 5 4 00 0. 085 85 0.85 079 79 0.79 072 72 0.72 16. 979 97.9 96.1 2 08 8 0.8 07 7 0.7 97. 96.

Abstract The objective of this experiment was to assess the effects of dietary sodium selenite and selenium (Se) yeast levels on growth performance, meat quality and muscle anti-oxidative capacity of broilers. A total of 360 1-day-old Cobb broilers were randomly assigned to 5 dietary treatments for 6 weeks. Diets (corn-soybean meal based diets) were supplemented with 0 mg/kg, 0.3 mg/kg and 0.6 mg/kg sodium selenium, and 0.3 mg/kg and 0.6 mg/kg selenium yeast, respectively. The results showed that Feed/Gain was significantly decreased with the increase of Se supplemental level during day 1-21 (P < 0.05), and body weight gain and Feed/Gain of broilers were improved with the increase of Se supplemental level by day 22 to 42 (P < 0.05). The dietary Se levels significantly affected the a*, cohesion, adhesion, chewing and recovery values of meat as well (P < 0.05). Se supplementation in diets also increased DPPH, hydroxyl radical scavenging and SOD, but significantly decreased TBARS and POV values compared to control (P < 0.05). The biological utilization rates of organic Se against inorganic Se were 116.0%, 123.1%, 109.7%, 109.8%, and 135.2%, respectively. The present results supported that organic Se was more bio-available and more effective in terms of growth performance, meat quality, muscle antioxidant and organ deposition efficiency compared to inorganic selenium. Therefore, the addition of 0.6 mg/kg selenium yeast (SY) during broiler breeding was the most effective. (Se performance antioxidative anti oxidative 36 1dayold dayold 1 old weeks cornsoybean corn soybean mgkg mg kg 03 3 0. 06 respectively FeedGain Feed Gain 121 21 1-2 P 0.05, 005 0.05 , 05 0.05) 2 4 0.05. . a a* cohesion adhesion DPPH SOD 1160 116 116.0% 1231 123 123.1% 1097 109 7 109.7% 1098 8 109.8% 1352 135 135.2% bioavailable bio available Therefore SY (SY 12 1- 00 0.0 11 116.0 123.1 10 109.7 109.8 13 135.2 116. 123. 109. 135.

RESUMO Introdução Como um novo método de treinamento, o método de treinamento de força por vibração tem sido amplamente utilizado no treinamento de força e na reabilitação em massa de atletas estrangeiros. Como método auxiliar de treinamento de força na China, a força vibratória também tem atraído a atenção dos profissionais. Estudos nessa área são importantes para acompanhar as mais recentes teorias e técnicas internacionais de treinamento de força por vibração. Objetivo Estudar os efeitos do treinamento de força por vibração nas articulações de membros inferiores de atletas de salto em distância. Métodos Fez-se uma pesquisa na literatura médica recente para desenvolver um método de teste experimental e implementou-se a estatística matemática para analisar este estudo de pesquisa com saltadores em distância voluntários. Resultados O aumento relativo do pico de torque das articulações do quadril e joelho foi maior quando comparado a articulação do tornozelo. Ambos demonstraram melhora considerável após o protocolo experimental. Conclusão Após comparar a força de vibração das articulações dos membros inferiores com o efeito de treinamento de atletas de salto em distância, alguns métodos de treinamento de força de flexor podem ser adicionados, aumentando adequadamente a intensidade e a quantidade de carga, de modo que o extensor da articulação dos membros inferiores e o nível de força de flexor possam ser coordenados proporcionalmente, visando aprimorar o nível de força e de coordenação motora nos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction As a new training method, vibration strength training has been widely used in strength training and mass rehabilitation of foreign athletes. As an auxiliary method of strength training in China, vibration strength has also attracted the attention of professionals. Studies in this area are important to keep up with the latest international theories and techniques of vibration strength training. Objective Study the effects of vibration strength training on the lower limb joints of long jump athletes. Methods A search of recent medical literature was conducted to develop an experimental test method, and mathematical statistics were implemented to analyze this research study with volunteer long jumpers. Results The relative increase in peak torque of the hip and knee joints was greater when compared to the ankle joint. Both showed considerable improvement after the experimental protocol. Conclusion After comparing the vibration force of lower limb joints with the training effect of long jump athletes, some flexor strength training methods can be added, increasing the intensity and the amount of load appropriately so that the lower limb joint extensor and flexor strength level can be coordinated proportionally, aiming to improve the level of strength and motor coordination in athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción Como nuevo método de entrenamiento, el método de entrenamiento de fuerza por vibración ha sido ampliamente utilizado en el entrenamiento de fuerza y la rehabilitación de masas de los atletas extranjeros. Como método auxiliar de entrenamiento de fuerza en China, la fuerza vibratoria también ha atraído la atención de los profesionales. Los estudios en este ámbito son importantes para mantenerse al día con las últimas teorías y técnicas internacionales de entrenamiento de fuerza por vibración. Objetivo Estudiar los efectos del entrenamiento de fuerza por vibración en las articulaciones de las extremidades inferiores de los atletas de salto de longitud. Métodos Se realizó una búsqueda en la literatura médica reciente para desarrollar un método de prueba experimental y se implementó la estadística matemática para analizar este estudio de investigación con saltadores de longitud voluntarios. Resultados El aumento relativo del par máximo de las articulaciones de la cadera y la rodilla fue mayor en comparación con la articulación del tobillo. Ambos mostraron una mejora considerable tras el protocolo experimental. Conclusión Después de comparar la fuerza de vibración de las articulaciones de los miembros inferiores con el efecto del entrenamiento de los atletas de salto de longitud, se pueden añadir algunos métodos de entrenamiento de la fuerza de los flexores, aumentando adecuadamente la intensidad y la cantidad de carga, de modo que el nivel de fuerza de los extensores de la articulación de los miembros inferiores y el nivel de fuerza de los flexores puedan coordinarse proporcionalmente, con el objetivo de mejorar el nivel de fuerza y la coordinación motora de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Abstract Clove is a kind of food spice, which can be used as food flavoring additive. In this work, we looked at clove's impact and underlying mechanism on cognitive impairment in infected mice. By using the open field test, Morris water maze, ELISA technique, kit method, and qPCR method, the biochemical and molecular biological indices of mice were identified. Clove component composition was established using HPLC. The findings demonstrated that clove might enhance mouse survival rates in comparison to the model group and could also lengthen driving distances, standing and combing periods, target quadrant duration, and crossing periods. In the hippocampus of infected mice, clove can lower levels of TNF-α, IL-6, IL-1β, and MDA while increasing SOD and CAT activity. In the hippocampus of infected animals, clove additionally increased the mRNA expression of SIRT1 and FOXO1 and decreased the mRNA expression of NF-B and p53. Animal studies revealed that clove's impact was comparable to dexamethasone in that it might help septic mice with cognitive impairment. The analysis revealed that rutin, isoquercitrin, ferulic acid, dihydroquercetin, and quercitrin were all present in clove. It is clear that clove can help septic mice with cognitive impairment by triggering SIRT1 through its active components.

Resumo Fundamentos: A denervação simpática renal por radiofrequência (DSR) mostra redução eficaz da pressão arterial (PA) de pacientes hipertensos, ainda que os mecanismos específicos permaneçam obscuros. Objetivo: Fizemos a hipótese de que níveis alterados de noradrenalina (NA) e mudanças nas enzimas relacionadas à NA e enzima conversora de angiotensina 2 (ECA-2), angiotensina (Ang)-(1-7) e receptor Mas são mediadores dos efeitos antihipertensivos da DSR. Métodos: Foram avaliados os valores médios de pressão arterial sistólica (PAS), pressão arterial diastólica (PAD) e pressão arterial média (PAM) no início e durante o seguimento. Foram medidas as concentrações plasmática e renal de noradrenalina (NA) por cromatografia líquida de alta eficiência com detecção eletroquímica, e os níveis de enzima relacionada à NA e ECA2-Ang-(1-7)-Mas através de PCR em tempo real, Western blot e imunohistoquímica ou Elisa em um modelo canino de hipertensão que recebeu ração rica em gordura e foi tratado com DSR. Os parâmetros também foram determinados em um grupo de cirurgia simulada submetido à arteriografia renal e em um grupo controle que recebeu dieta normal. Resultados: DSR causou diminuição da PAS, PAD, PAM e das concentrações plasmática e renal de NA. Em comparação ao grupo placebo, a expressão da tirosina hidroxilase (TH) renal foi menor e a da renalase foi maior no grupo DSR. Em comparação ao grupo controle, os níveis de TH renal e de catecol-o-metil-transferase (COMT) foram maiores e os de renalase foram menores no grupo cirurgia simulada. Além disso, os níveis renais de ECA2, Ang-(1-7) e Mas foram maiores no grupo DSR do que no grupo cirurgia simulada, que, por sua vez, foram menores do que no grupo controle. Conclusões: A DSR mostra efeitos anti-hipertensivos com redução da NA e ativação da ECA2-Ang-(1-7)-Mas, o que indica que pode contribuir com o efeito anti-hipertensivo da DSR.

Abstract Background: Radiofrequency ablation of renal sympathetic nerve (RDN) shows effective BP reduction in hypertensive patients while the specific mechanisms remain unclear. Objective: We hypothesized that abnormal levels of norepinephrine (NE) and changes in NE-related enzymes and angiotensinconverting enzyme 2 (ACE2), angiotensin (Ang)-(1-7) and Mas receptor mediate the anti-hypertensive effects of RDN. Methods: Mean values of systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP) were assessed at baseline and follow-up. Plasma and renal norepinephrine (NE) concentrations were determined using highperformance liquid chromatography with electrochemical detection, and levels of NE-related enzyme and ACE2-Ang(1-7)- Mas were measured using real time PCR, Western blot and immunohistochemistry or Elisa in a hypertensive canine model fed with high-fat diet and treated with RDN. The parameters were also determined in a sham group treated with renal arteriography and a control group fed with normal diet. Results: RDN decreased SBP, DBP, MAP, plasma and renal NE. Compared with the sham group, renal tyrosine hydroxylase (TH) expression was lower and renalase expression was higher in the RDN group. Compared with the control group, renal TH and catechol-o-methyl transferase (COMT) were higher and renalase was lower in the sham group. Moreover, renal ACE2, Ang-(1-7) and Mas levels of the RDN group were higher than those of the sham group, which were lower than those of the control group. Conclusion: RDN shows anti-hypertensive effect with reduced NE and activation of ACE2-Ang(1-7)-Mas, indicating that it may contribute to the anti-hypertensive effect of RDN.

The polymorphism in the gonadotropin-releasing hormone receptor (GnRHR) gene was detected by PCR single-strand conformational (PCR-SSCP) and DNA sequencing methods in 224 Boer goats. Two single nucleotide polymorphisms (SNPs), G891T and G757A, were found in the exon I of the goat GnRHR gene and their associations with litter size in Boer goats were evaluated. Association analysis showed that G891T and G757A had significant effects on litter size. In locus G891T, individuals with GT genotype had significantly larger litter sizes compared to GG genotype in the first and third parity. In locus G757A, ewes with GG genotype had significantly higher litter sizes than those of GA in the fourth parity. The present study primarily revealed an association between allele T at 891 locus of the GnRHR gene and high litter size in the first and third parity in Boer goats. These results provide evidence that the GnRHR gene may be significantly correlated with a high prolificacy in goats.