Abstract The ground beetle, Neoaulacoryssus speciosus (Coleoptera: Carabidae) is of high relevance to field because it has been recorded as a pest of seeds and young plants of vegetables and other crops and a predator in agricultural crops, forest and weeds in Brazil. However, natural habitat changes are increasing agriculture and forest insect outbreaks in urban areas. A N. speciosus population outbreak occurred in October and November 2018 simultaneously in 12 neighboring municipalities at the beginning of the rainy season in the northern region of Minas Gerais State, Brazil. The objectives of this study were to report a sudden and simultaneous population outbreak of N. speciosus and to describe the factors of habitat change that could have contributed to this invasion in 12 municipalities in the northern region of Minas Gerais State in the Caatinga biome of Brazil. In addition, female and male genitals were described and illustrated, the scientific classification revised and common names of N. speciosus listed. Thousands of males and females of N. speciosus agglomerated in shady, humid places during the day and night for about 15 days. Neoaulacoryssus speciosus has been identified and illustrated, its scientific classification revised and four common names listed for this species. beetle Coleoptera (Coleoptera Carabidae Brazil However areas N 201 1 addition illustrated shady days species 20 2

Abstract The corn cropping system can influence the natural enemy identity and the number of Spodoptera frugiperda J.E. Smith, 1797 (Lepidoptera: Noctuidae) individuals infesting the cartridge, ear and stem of this plant. The objectives were to identify the S. frugiperda natural enemies and differences in the number of individuals infesting the cartridge, ear and stem of corn plants under conventional and organic cropping systems, in Brazil after an initial collection of adult males through semiochemical traps. We also evaluated key morphometrical parameters of the larva and factors contributing with the viability of S. frugiperda. A total of 16 and 136 adult males was trapped, and 1,124 and 1,112 larvae was recovered from conventional and organic systems with 4.7 and 6.7% of them parasitized by dipteran and hymenopteran, respectively. Most of the parasitoids recovered had occurrence in both cropping systems, except Cremastinae and Ophion flavidus Brullé, 1846 (Hymenoptera: Ichneumonidae), which were recorded only in organic and Campoletis sp. (Hymenoptera: Ichneumonidae) only in conventional corns. The number of parasitoids recovered was similar in corn plant samples between both cropping systems. A total of 152 and 31 larvae was recovered from corn ears under conventional and organic systems, respectively. Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) was recovered from all samples under conventional system. The larva length was overall similar between cropping systems. Parasitism by dipteran and hymenopteran, infection by microorganisms, larva mortality by undetermined causes, and differences in viability of S. frugiperda stages were factors contributing with the supression of this pest. The new associations and parasitoids reported represent possibilities of expanding the biological control strategies to manage S. frugiperda in corn crops. JE J E J.E Smith 179 Lepidoptera (Lepidoptera Noctuidae cartridge S traps 1 13 trapped 1124 124 1,12 1112 112 1,11 47 4 7 4. 67 6 6.7 hymenopteran respectively Brullé 184 Hymenoptera (Hymenoptera Ichneumonidae, Ichneumonidae , sp corns 15 3 Scudder, Scudder (Scudder 1876 Dermaptera (Dermaptera Forficulidae microorganisms causes pest crops 17 12 1,1 111 11 6. 18 187 1,

Abstract The use of biopesticides based on Bacillus thuringiensis (Berliner 1915) in agriculture has been considered harmless for non-target organisms such as fish. The present study aimed to investigate the effects of a biopesticide based on B. thuringiensis on the physiology and histology of the liver, kidney and intestine of Arapaima gigas (Schinz, 1822), via exposure to water (0.13 g/L) and in the diet (0.13 g), after 24 and 48 h. Fish subjected to B. thuringiensis in the water of their breeding and feeding tanks exhibited mortality due to changes in erythrogram (hematocrit, hemoglobin, erythrocytes), thrombogram and leucogram blood parameters, and plasma (sodium, chloride, potassium, cholesterol, glucose, triglycerides, cortisol and total proteins) and enzymatic (Aspartate Amino Transferase and Alanine Amino Transferase) biochemistry. Histopathological alterations in the liver and kidney ranged from mild to severe and were characterized by the presence of cytoplasmic vacuolization, nuclear hypertrophy and atrophy, melanomacrophage centers and necrosis, and in the intestine by changes to the number of villi and goblet cells. Therefore, these physiological and histopathological alterations indicate that care should be taken with the dispersion of biopesticides based on agricultural B. thuringiensis in fish farming.

Abstract: This study investigated the physiological effects of a pesticide based on Bacillus thuringiensis (Dipel-WP®) added in the water and diet of Piaractus mesopotamicus during 24 and 48 h. It was added 0.13 g of de B. thuringiensis per kg of commercial feed; and for the fish subjected to the biopesticide in the water of the tanks, it was added 0.13 g/L of the biopesticide. Plasma levels of sodium, chloride, potassium, cholesterol, glucose, triglycerides, cortisol, total protein, alanine aminotransferase (ALT), aspartate aminotransferase (AST), hematocrit, hemoglobin, erythrocytes number, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), number of thrombocytes and leukocytes were determined. Cortisol, lactate, glucose, total protein, cholesterol, triglycerides, ALT, AST, sodium, potassium, hematocrit, hemoglobin, MCV, number of erythrocytes, leukocytes, lymphocytes, eosinophils and PAS-positive granular leukocytes suffered alterations derived from the addition of B. thuringiensis in water and diet of the fish. The toxicity of the concentrations of biopesticide in short-term exposure in water and diet of the fish led to blood alterations (increase or decrease). Therefore, care must be taken to avoid a possible prolonged contamination in the tanks of fish farming by agricultural pollution based on B. thuringiensis.

Resumo: O objetivo deste trabalho foi avaliar a distribuição espacial e temporal de Sarsina violascens (Lepidoptera: Noctuidae: Lymantriinae) em duas plantações de Eucalyptus cloeziana, uma com faixas de vegetação nativa (CFVN) e outra sem (SFVN). Adultos foram coletados com armadilhas luminosas, as quais foram instaladas: no interior de uma área com vegetação nativa (Cerrado), a 100 m da borda; na zona de contato entre a vegetação nativa e a plantação de E. cloeziana; no interior da plantação de E. cloeziana, a 250 m da borda; na parte central da faixa de vegetação nativa, cerca de 500 m da borda (CFVN) ou na zona de contato entre dois talhões de E. cloeziana (SFVN); e no interior da plantação de E. cloeziana, a 500 m da borda. O número de adultos de S. violascens coletados foi de 240 no sistema CFVN e de 1.378 no sistema SFVN. O menor número de indivíduos no sistema CFVN provavelmente foi devido ao favorecimento do controle biológico pelo aumento da riqueza de espécies com o uso de faixas de vegetação nativa. Essas faixas, intercaladas com plantações de E. cloeziana, possibilitam maior proporção de floresta nativa na paisagem e podem auxiliar na redução de infestações por S. violascens.

Abstract: The objective of this work was to evaluate spatial and temporal distributions of Sarsina violascens (Lepidoptera: Noctuidae: Lymantriinae) in two Eucalyptus cloeziana plantations, one with native vegetation strips (WNVS) and another without them (ONVS). Adults were collected with light traps, which were installed: inside an area of native vegetation (Cerrado), 100 m from the edge; at the contact zone between the native vegetation area and the E. cloeziana plantation; inside the E. cloeziana plantation, 250 m from the edge; at the central part of the native vegetation strip, around 500 m from the edge (WNVS) or in the contact zone between two E. cloeziana compartments (ONVS); and inside the E. cloeziana plantation, 500 m from the edge. The number of S. violascens adults collected was 240 in the system WNVS and 1,378 in the system ONVS. The lower number of individuals in the system WNVS was probably due to favored biological control provided by higher species richness with the use of native vegetation strips. These strips, intermingled with E. cloeziana plantations, allow a higher proportion of native forest in the landscape and can help to reduce S. violascens infestations.

A região da Zona da Mata está localizada no sudeste do estado de Minas Gerais, Brasil com fauna e flora diversificadas, incluindo insetos herbívoros e plantas Solanaceae. Lagartas Ithomiinae foram observadas danificando árvore de Solanum granuloso-leprosum Dunal (Solanaceae), usada para diversos fins e abundante em floresta secundária. Objetivou-se, com este estudo, identificar lagartas desfolhadoras de S. granuloso-leprosum no campus da Universidade Federal de Viçosa (UFV) em Viçosa, Minas Gerais, Brasil e revisar plantas hospedeiras de Mechanitis polymnia (L., 1758) (Lepidoptera: Nymphalidae). Treze lagartas encontradas danificando uma árvore de S. granuloso-leprosum no campus da UFV foram coletadas e mantidas no Laboratório de Controle Biológico de Insetos (LCBI) da UFV até a emergência de adultos. Essas lagartas foram de duas espécies, sendo dez da primeira e três da segunda espécie. Espécimes adultos da última espécie foram identificados como Ithomia lichyi lichyi D'Almeida, 1939 (Lepidoptera: Nymphalidae) no Departamento de Zoologia da Universidade Federal do Paraná (UFPR) em Curitiba, Paraná, Brasil e do grupo de dez lagartas como Mechanitis polymnia casabranca Haensch, 1905 (Lepidoptera: Nymphalidae) no Museu de Zoologia da Universidade de São Paulo (USP) em São Paulo, Brasil. Esse é o primeiro relato de M. polymnia casabranca e I. lichyi lichyi juntos, danificando planta de S. granuloso-leprosum, na região da Zona da Mata, de Minas Gerais, Brasil e 57 plantas estão registradas como hospedeiras de M. polymnia.

The Zona da Mata region is located in southeastern Minas Gerais State, Brazil with fauna and flora diversified, including herbivorous insects and Solanaceae plants. Ithomiinae caterpillars were observed damaging tree of Solanum granuloso-leprosum Dunal (Solanaceae), used for different purposes and abundant in secondary forest. The objective of this study was to identify defoliating caterpillars of S. granuloso-leprosum at the campus of Universidade Federal de Viçosa (UFV) in Viçosa, Minas Gerais State, Brazil and review host plants of Mechanitis polymnia L., 1758 (Lepidoptera: Nymphalidae). Thirteen caterpillars found damaging a tree of S. granuloso-leprosum at the campus of UFV were collected and maintained in the Laboratório de Controle Biológico de Insetos (LCBI) from UFV until adult emergence. These caterpillars were of two species, being ten of the first and three of the second species. Adult specimens of the latter species were identified as Ithomia lichyi lichyi D'Almeida, 1939 (Lepidoptera: Nymphalidae) in the Departamento de Zoologia of Universidade Federal do Paraná (UFPR) in Curitiba, Paraná State, Brazil and of the group of ten caterpillars as Mechanitis polymnia casabranca Haensch, 1905 (Lepidoptera: Nymphalidae) in the Museu de Zoologia of Universidade de São Paulo (USP) in São Paulo State, Brazil. This is the first report of M. polymnia casabranca and I. lichyi lichyi together damaging plant of S. granuloso-leprosum in the Zona da Mata region of Minas Gerais State, Brazil and 57 plants are recorded as host of M. polymnia.

Respostas funcional e numérica, características reprodutivas e viabilidade de Campoletis flavicincta (Hymenoptera: Ichneumonidae) e mortalidade após o parasitismo do hospedeiro Spodoptera frugiperda (Lepidoptera: Noctuidae) foram estudados em laboratório. Casais de C. flavicincta foram mantidos até a morte da fêmea com 10, 20, 30, 40 ou 50 lagartas dia-1 do hospedeiro S. frugiperda. Um tipo de curva de resposta funcional III foi ajustado para o número médio de lagartas fornecidas por dia, durante a vida da vespa fêmea, como a variável explicativa. Tempo de tratamento foi 0,5940 ± 0,0875h e taxa de busca instantânea 0.0047 ± 0.0020 h-1. A resposta funcional para cada um dos cinco primeiros dias de fornecimento do hospedeiro foi do tipo III. Longevidade nas cinco densidades do hospedeiro e taxa de parasitismo mostraram redução linear significativa com a densidade do hospedeiro. Produção de descendentes mostrou variação quadrática crescente com a densidade crescente do hospedeiro. Produção de fêmeas de C. flavicincta, razão sexual de descendentes, viabilidade de pupas do parasitóide e porcentagem de mortalidade de lagartas de S. frugiperda não foram afetados pela densidade do hospedeiro. Respostas funcional e numérica de C. flavicincta indicam que esse parasitóide pode ser um candidato para controle biológico de S. frugiperda.

The functional and numerical responses, reproductive characteristics, and viability of Campoletis flavicincta (Hymenoptera: Ichneumonidae) as well as the mortality after parasitism of the host Spodoptera frugiperda (Lepidoptera: Noctuidae) were analyzed in the laboratory. Campoletis flavicincta pairs were maintained until female death with 10, 20, 30, 40, or 50 caterpillars day-1 of the host S. frugiperda. A type III functional response curve was fitted to the average number of caterpillars supplied per day during the female wasp lifespan, as the explanatory variable. The handling time was 0.5940 ± 0.0875h, and the instantaneous search 0.0047 ± 0.0020 h-1. The functional response for each of the first five days of the host was a type III. The longevity at the five host densities and the parasitism rate showed a significant linear decrease with the host density. The offspring production showed an increasing quadratic variation with increased host density. The production of females by C. flavicincta, the offspring sex ratio, the viability of the parasitoid pupae and the percentage of mortality of S. frugiperda caterpillars were not affected by host density. The functional and numerical responses of C. flavicincta indicate that this parasitoid could be a candidate for biological control of S. frugiperda.

Com o objetivo de inventariar os metazoários endoparasitos em Serrasalmus marginatus, no Rio Negro, Pantanal, Mato Grosso do Sul, Brasil, foram examinados 91 espécimes capturados no período de outubro de 2007 a agosto de 2008. Foram registrados seis táxons parasitos: Procamallanus (Spirocamallanus) inopinatus, Contracaecum sp. (Nematoda), metacercária do tipo Diplostomulum (Digenea); Brevimulticaecum sp. (Nematoda); e Sebekia oxycephala, Subtriquetra sp. 1 e Subtriquetra sp. 2 (Pentastomida). As últimas cinco espécies citadas são registradas pela primeira vez em S. marginatus.

In order to inventory the metazoan endoparasites of Serrasalmus marginatus, 91 specimens were examined. They were captured in the Negro River in Pantanal wetland, State of Mato Grosso do Sul, Central-Western Brazil, from October 2007 to August 2008. Parasites of six taxa were recovered: Procamallanus (Spirocamallanus) inopinatus, Contracaecum sp. (Nematoda), metacercarial type Diplostomulum (Digenea), Brevimulticaecum sp. (Nematoda) and Sebekia oxycephala, Subtriquetra sp. 1 and Subtriquetra sp. 2 (Pentastomida). The latter five species are reported for the first time in S. marginatus.