Abstract Cigarette smoke (CS) has been generally recognized as a chief carcinogenic factor in renal cell carcinoma (RCC). The stimulative effect of CS on renal cancer stem cells (RCSCs) has been described previously. The Sonic Hedgehog (SHH) pathway plays an essential role in self-renewal, cell growth, drug resistance, metastasis, and recurrence of cancer stem cells (CSCs). Renal cancer-related gene ΔNp63α is highly expressed in renal epithelial tissues and contributes to the RCSCs characteristics of tumors. The aim of this study was to elucidate the role of ΔNp63α and the SHH pathway on the activity of RCSCs induced by CS through a series of in vivo and in vitro studies. It was shown that in renal cancer tissues, ΔNp63α and RCSCs markers in smokers are expressed higher than that in non-smokers. RCSCs were effectively enriched by tumor sphere formation assay. Besides, CS increased the expression of RCSCs markers and the capability of sphere-forming in vitro and in vivo. Moreover, the SHH pathway was activated, and the specialized inhibitor alleviated the promotion of CS on RCSCs. ΔNp63α activated the SHH pathway and promoted CS-induced enhancement of RCSCs activity. These findings indicate that ΔNp63α positively regulates the activity of CS-induced RCSCs via the SHH pathway. (CS RCC. RCC . (RCC) (RCSCs previously (SHH selfrenewal, selfrenewal self renewal, renewal self-renewal growth resistance metastasis CSCs. CSCs (CSCs) cancerrelated related ΔNpα ΔNp α tumors studies nonsmokers. nonsmokers non smokers. non-smokers assay Besides sphereforming forming Moreover CSinduced (RCC (CSCs

Abstract: The privacy problem of facial recognition technology is that commercial companies obtain people’s facial information without the consent of individuals and use facial information to infringe on the privacy of individuals. The importance of human privacy in facial recognition technology is reflected through facial ethics, which requires others to perform corresponding obligations to individuals, such as oral care. Through the analysis of the privacy issues of facial recognition technology, it is found that the two elements of “without personal informed” and “without personal consent” together form the basis for commercial companies to violate personal privacy. The principle of informed consent includes the principle of informed and the principle of consent, which is derived from the principle of informed consent in medical ethics. This paper improves the principles of informed consent in medicine and ethics to better address facial recognition privacy issues.

Resumen: El problema de la privacidad en la tecnología de reconocimiento facial es que las empresas comerciales obtienen información facial de las personas sin el consentimiento de éstas y utilizan la información facial para vulnerar la privacidad de las personas. La importancia de la privacidad de las personas en la tecnología de reconocimiento facial se refleja a través de la ética facial, que exige que otros cumplan las obligaciones correspondientes con los individuos, como el cuidado bucal. A través del análisis de los problemas de privacidad de la tecnología de reconocimiento facial se descubre que los dos elementos de “sin información personal” y “sin consentimiento personal” juntos forman la base para que las empresas comerciales violen la privacidad personal. El principio de consentimiento informado incluye el de información y el de consentimiento, que se deriva del principio de consentimiento informado de la ética médica. Este artículo mejora los principios del consentimiento informado en medicina y ética para abordar mejor los problemas de privacidad del reconocimiento facial.

Resumo: A questão da privacidade na tecnologia de reconhecimento facial é que as companhias comerciais obtém informações faciais das pessoas sem seu consentimento e usam informação facial para infringir sua privacidade. A importância da privacidade humana na tecnologia de reconhecimento facial é refletida através da ética facial, que exige que se cumpram obrigações correspondentes para com os indivíduos, da mesma forma como com cuidados orais. Através da análise de aspectos de privacidade na tecnologia de reconhecimento facial, encontrou-se que os dois elementos “sem informação pessoal” e “sem consentimento pessoal” juntos, formam a base para companhias comerciais violarem a privacidade pessoal. O princípio do consentimento informado inclui o princípio de informação e o princípio de consentimento, os quais derivam do princípio do consentimento informado em ética médica. Esse artigo melhora os princípios do consentimento informado em medicina e ética para melhor incluir aspectos de privacidade no reconhecimento facial.

Objective: Mortality rate is a general indicator which can be used to measure care and management of schizophrenia. This cohort study evaluated the standardized mortality ratios (SMRs) of all-cause mortality and life-years lost (LYLs) in patients with schizophrenia under a community care program in China. Methods: Data were obtained from the National Community Care Program System for Severe Mental Disorders. A total of 99,214 patients diagnosed with schizophrenia were enrolled before December 2014 and followed between 2015 and 2019. A total of 9,483 patients died. Crude mortality rates (CMRs) and SMRs were then stratified by natural vs. unnatural causes, and major groups of death were standardized according to the 2010 National Population SMRs. The corresponding LYLs at birth were also calculated by gender and age. Results: The SMRs of patients with schizophrenia were significantly elevated during the study period, with an overall SMR of 4.98 (95%CI 2.67-7.32). Neoplasms, cardiovascular diseases, cerebrovascular diseases, external injuries, and poisonings were the most significant causes of death among patients with schizophrenia compared to the general population. The mean LYLs of patients with schizophrenia were 15.28 (95%CI 13.26-17.30). Males with schizophrenia lost 15.82 life-years (95%CI 13.48-18.16), and females lost 14.59 life-years (95%CI 13.12-16.06). Conclusions: Patients with schizophrenia under community care had a high mortality rate in our study, even though mental health services have been integrated into the general healthcare system in China to narrow treatment gaps in mental health for > 10 years. In terms of mortality outcome indicators, effective and quality mental health services still have a long way to go. The current study demonstrates the potential for improved prevention and treatment of individuals with schizophrenia under community care. Objective (SMRs allcause all cause lifeyears life years (LYLs Methods Disorders 99214 99 214 99,21 201 2019 9483 9 483 9,48 died CMRs (CMRs vs age Results period 498 4 98 4.9 95%CI 95CI CI 95 2.677.32. 267732 2.67 7.32 . 2 67 7 32 2.67-7.32) Neoplasms diseases injuries population 1528 15 28 15.2 13.2617.30. 13261730 13.26 17.30 13 26 17 30 13.26-17.30) 1582 82 15.8 13.4818.16, 13481816 13.48 18.16 , 48 18 16 13.48-18.16) 1459 14 59 14.5 13.1216.06. 13121606 13.12 16.06 12 06 13.12-16.06) Conclusions 1 indicators go 9921 21 99,2 20 948 9,4 49 4. 677 2.677.32 26773 267 2.6 732 7.3 6 3 2.67-7.32 152 15. 2617 13.2617.30 1326173 1326 13.2 1730 17.3 13.26-17.30 158 8 4818 13.4818.16 1348181 1348 13.4 1816 18.1 13.48-18.16 145 5 14. 1216 13.1216.06 1312160 1312 13.1 1606 16.0 0 13.12-16.06 992 99, 94 9, 2.677.3 2677 2. 73 7. 2.67-7.3 261 13.2617.3 132617 132 13. 173 17. 13.26-17.3 481 13.4818.1 134818 134 181 18. 13.48-18.1 121 13.1216.0 131216 131 160 16. 13.12-16.0 2.677. 2.67-7. 13.2617. 13261 13.26-17. 13.4818. 13481 13.48-18. 13.1216. 13121 13.12-16. 2.677 2.67-7 13.2617 13.26-17 13.4818 13.48-18 13.1216 13.12-16 2.67- 13.261 13.26-1 13.481 13.48-1 13.121 13.12-1 13.26- 13.48- 13.12-

Abstract This work studied the effects of different time and temperature combinations [100 °C for 50 min (T1), 110 °C for 50 min (T2), 118 °C for 50 min (T3) and 121 °C for 30 min (T4)] of sterilization on the sensory quality and lipid oxidation of Dezhou braised chicken. Compared to T1, T3 and T4 groups, the T2 group had the highest colour and lower hardness. The principal component analysis (PCA) of the electronic-tongue results showed that there were distinct differences in taste between the treatment groups. Free amino acids (FAA) analysis showed that the T2 group had the highest amino acid contents. In summary, the T2 might be a more reasonable sterilization condition for Dezhou braised chicken, due to its positive effects on maintaining sensory quality. The results of this study could guide Dezhou braised chicken industry to produce better quality products. 100 [10 C 5 T1 T , (T1) 11 T2, (T2) (T3 12 3 (T4) groups hardness PCA (PCA electronictongue electronic tongue FAA (FAA contents summary products 10 [1 (T1 1 (T2 (T (T4 [

Abstract Background Subarachnoid hemorrhage (SAH) is an uncommon and serious subtype of stroke, which leads to the loss of the patient’s ability to produce and live for many years. Objective To investigate the clinical effect of nimodipine in the treatment of SAH. Methods Electronic databases including China National Knowledge Infrastructure (CNKI), VIP, SinoMed, China Master’s Theses Full-text Database (CMFD), China Doctoral Dissertations Full-text Database (CDFD), Cochrane Library, PubMed and Embase were searched from 2010 and 2021. All randomized controlled trials evaluating the efficacy of nimodipine in the treatment of SAH were included in our meta-analysis. The patients were divided into control group and treatment group. Meta-analysis was performed with Stata16.0 software. Results A total of 10 studies were included. Compared with the control group, the treatment group had higher effective rate (OR = 3.21, 95% CI: 2.25, 4.58; p < 0.001), and lower incidence of adverse reactions (OR = 0.35, 95% CI: 0.19, 0.67; p = 0.001). Before treatment, no significant differences were identified in middle cerebral artery blood flow velocity and Glasgow coma scale (GCS) score between the two groups. However, after treatment, the middle cerebral artery blood flow velocity (SMD = — 1.36, 95% CI: —2.28, —0.49; p = 0.002) and GCS score (SMD = 1.24, 95% CI: 0.58, 1.89; p < 0.001) in the treatment group were significantly better than those in the control group. Conclusions Nimodipine is effective in the treatment of SAH, lowering incidence of adverse reactions and therefore improving the prognosis of patients. (SAH stroke patient s years CNKI, CNKI , (CNKI) VIP SinoMed Masters Master Fulltext Full text CMFD, CMFD (CMFD) CDFD, CDFD (CDFD) Library 201 2021 metaanalysis. metaanalysis meta analysis. analysis meta-analysis Metaanalysis Meta Stata160 Stata Stata16 0 Stata16. software 1 OR 321 3 21 3.21 95 CI 225 2 25 2.25 4.58 458 4 58 0.001, 0001 0.001 001 035 35 0.35 019 19 0.19 0.67 067 67 0.001. . (GCS groups However SMD 136 36 1.36 2.28, 228 28 —2.28 0.49 049 49 —0.49 0.002 0002 002 124 24 1.24 058 0.58 1.89 189 89 (CNKI (CMFD (CDFD 20 202 Stata1 32 3.2 9 22 2.2 4.5 45 5 000 0.00 00 03 0.3 01 0.1 0.6 06 6 13 1.3 2.28 —2.2 0.4 04 —0.4 12 1.2 05 0.5 1.8 18 8 3. 2. 4. 0.0 0. 1. —2. —0. —2 —0

Resumo Antecedentes Hemorragia subaracnóidea (SAH) é um subtipo raro e grave de acidente vascular cerebral (AVC), o que leva à perda da capacidade do paciente de produzir e viver por muitos anos. Objetivo Investigar o efeito clínico da nimodipina no tratamento da SAH. Métodos As bases de dados eletrônicas, incluindo a China National Knowledge Infrastructure (CNKI), VIP, SinoMed, Masters Theses Full-text Database (CMFD), China Doctoral Dissertations Full-text Database (CDFD), Cochrane Library, PubMed e Embase foram pesquisadas no período de 2010 a 2021. Todos os ensaios controlados aleatorizados que avaliam a eficácia da nimodipina no tratamento da SAH foram incluídos na nossa meta-análise. Os pacientes foram divididos em grupo controle e grupo de tratamento. Meta-análise foi realizada com o software Stata 16.0. Resultados Foram incluídos um total de dez estudos. Em comparação com o grupo controle, o grupo de tratamento tinha uma taxa mais elevada (OR = 3,21, 95% CI: 2,25, 4,58; p < 0,001), e menor incidência de reações adversas (OR = 0,35, 95% CI: 0,19, 0,67; p = 0,001). Antes do tratamento, não foram identificadas diferenças significativas na velocidade média do fluxo sanguíneo da artéria cerebral e na pontuação de Glasgow coma scale (GCS) entre os dois grupos. No entanto, após o tratamento, a velocidade média do fluxo sanguíneo da artéria cerebral (SMD = −1,36, 95% CI: −2,28, 0,49; p = 0,002) e a pontuação do GCS (SMD = 1,24, 95% CI: 0,58, 1,89; p < 0,001) no grupo de tratamento foram significativamente melhores do que os do grupo controle. Conclusões A nimodipina é eficaz no tratamento da SAH, diminuindo a incidência de reações adversas e, consequentemente, melhorando o prognóstico dos doentes. (SAH AVC, AVC , (AVC) anos eletrônicas CNKI, CNKI (CNKI) VIP SinoMed Fulltext Full text CMFD, CMFD (CMFD) CDFD, CDFD (CDFD) Library 201 2021 metaanálise. metaanálise meta análise. análise meta-análise Metaanálise Meta 160 16 0 16.0 estudos OR 321 3 21 3,21 95 CI 225 2 25 2,25 4,58 458 4 58 0,001, 0001 0,001 001 035 35 0,35 019 19 0,19 0,67 067 67 0,001. . (GCS grupos entanto SMD 136 1 36 −1,36 228 28 −2,28 0,49 049 49 0,002 0002 002 124 24 1,24 058 0,58 1,89 189 89 consequentemente doentes (AVC (CNKI (CMFD (CDFD 20 202 16. 32 3,2 9 22 2,2 4,5 45 5 000 0,00 00 03 0,3 01 0,1 0,6 06 6 13 −1,3 −2,2 0,4 04 12 1,2 05 0,5 1,8 18 8 3, 2, 4, 0,0 0, −1, −2, 1, −1 −2 −

Abstract Gut bacterial β-glucuronidase (GUS) can reactivate xenobiotics that exert enterohepatic circulation- triggered gastrointestinal tract toxicity. GUS inhibitors can alleviate drug-induced enteropathy and improve treatment outcomes. We evaluated the inhibitory effect of Polygonum cuspidatum Siebold & Zucc. and its major constituents against Escherichia coli GUS (EcGUS), and characterized the inhibitory mechanism of each of the components. Trans-resveratrol 4’-O-β-D-glucopyranoside (HZ-1) and (-)-epicatechin gallate (HZ-2) isolated from P. cuspidatum were identified as the key components and potent inhibitors. These two components displayed strong to moderate inhibitory effects on EcGUS, with Ki values of 9.95 and 1.95 μM, respectively. Results from molecular docking indicated that HZ-1 and HZ-2 could interact with the key residues Asp163, Ser360, Ile 363, Glu413, Glu504, and Lys 568 of EcGUS via hydrogen bonding. Our findings demonstrate the inhibitory effect of P. cuspidatum and its two components on EcGUS, which supported the further evaluation and development of P. cuspidatum and its two active components as novel candidates for alleviating drug-induced damage in the mammalian gut. βglucuronidase β glucuronidase (GUS circulation toxicity druginduced drug induced outcomes Zucc , (EcGUS) Transresveratrol Trans resveratrol 4OβDglucopyranoside OβDglucopyranoside 4 O D glucopyranoside HZ1 HZ 1 (HZ-1 epicatechin HZ2 2 (HZ-2 P 995 9 95 9.9 195 1.9 μM respectively HZ- Asp163 Asp Ser360 Ser 363 Glu413 Glu Glu504 56 bonding gut (EcGUS (HZ- 99 9. 19 1. Asp16 Ser36 36 Glu41 Glu50 5 (HZ Asp1 Ser3 3 Glu4 Glu5

Abstract Evaluation of volatile aroma compounds from Chinese wild grape species aimed to explore new grape species via fruit aroma. In this study, the volatile organic compounds (VOCs) from ten grape cultivars belonging to nine different grape species were detected, by using headspace solid-phase microextraction with GC-MS. Chinese wild grape species were studied along with table grape (Muscat Hamburg, Concord) and wine grape (Cabernet Sauvignon) cultivars belonging to different origins. Total forty-five volatile compounds were detected, upon which several VOCs including pentanoic acid, cyclobutyl ester, maltol, (+)-neoisomenthol, and 8-methyloctahydrocoumarin were detected in Chinese wild grapes that have not been detected in other Vitis species. The results revealed that C6 volatile compounds were pre-dominant VOCs. Esters were found abundant in V. labrusca (Concord) and terpenoids were dominant in V. vinafera (Hamburg Muscat). The principal component analysis showed that Chinese wild grape cultivars V. yeshanensis, V. facifolia, V. davidii, V. betulifolia V. heyneana, V. amurensis and V. adenoclada are correlated with wine grape cultivar ‘Cabernet Sauvignon’, while table grape cultivars are distributed separately. These results can provide a reference for the utilization of new germplasm for specific purposes.

Abstract The extract of Belamcanda chinensis leaves (BCLE) is a traditional Chinese herbal medicine for the treatment of diabetes-related hyperlipidemia in Hainan province, South China. In this study, the lipid-decreasing effects of BCLE on obese diabetes were investigated on KK-Ay mice. The component F2 ameliorated lipid disorder, as indicated by decreased levels of body weight, liver index, levels of TC, TG and LDL-c in the serum and liver. The enhancement effect of F2 on liver SOD and the inhibitory effect of F2 on MDA demonstrated that F2 exhibited significant antioxidant activity on liver injury. F2 also prevented vacuolar degeneration and reduced pathological tissue injury in liver. In addition, the component F1 decreased the levels of TG, LDL-c and MDA in the liver. These findings suggest that F2 may have therapeutic potential in the prevention and therapy of hyperlipemia and liver disease associated with obesity-related diabetes.

The detection of vitamin D in complex samples is challenging because of the instability of vitamin D in sample preparation. To resolve this problem, packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D2 (D2) and vitamin D3 (D3) in dairy products. Based on PFSPE, vitamin D could avoid oxidation because of the shorter extraction time. The adsorbent materials, polystyrene/polypyrrole (PS/PPy) nanofibers, were prepared by electrospinning of PS and in situ polymerization of PPy on the surface of PS nanofibers. The linearity was within the range of 1-2000 ng mL−1 in dairy solution, and the recoveries were 91.9-109.1% with a relative standard deviation (RSD) < 13.6%. Therefore, the prepared method could be used for the detection of D2 and D3 in dairy products.

Abstract Accumulating evidence over the last two decades has established the causal role of a unidirectional orthography in shaping speakers, mental representations of time. Casasanto and Bottini (Journal of Experimental Psychology: General, 143, 473-479, 2014) extended previous findings by showing that exposure to mirror-reversed orthography of speakers' native language could completely redirect their mental timelines within minutes. However, the question of whether such a causal effect of writing direction on temporal cognition can be identified in speakers whose native languages adopt bidirectional orthographies remains underexplored in the literature. To address this issue, the present study focused on Japanese which uses bidirectional writing systems, one proceeding horizontally from left to right (HLR) and one vertically from top to bottom (VTB). Two experiments were performed, and the tasks asked participants to process standard/mirror orthography prime questions about time arranged horizontally or vertically, followed by horizontal or vertical arrays of pictorial target stimuli about temporal relations. Results demonstrated that Japanese speakers encoded passage of time into a top-to-bottom linear path commensurate with the VTB writing direction, but they did not align their mental representations of time with the HLR writing orientation. Accordingly, exposure to mirror-reversed bidirectional orthographies redirected Japanese speakers' vertical but not horizontal space-time mappings. Theoretical implications concerning the causal effects of bidirectional orthographies and the generalizability of the representational flexibility of time maintained by Casasanto and Bottini (Journal of Experimental Psychology: General, 143,473-479) are discussed.

Abstract Background: Circulating RNA (circRNA) regulates various bioactivities in cells. A better understanding of the exosomal circRNA can provide novel insights into the pathogenesis and treatment of Graves' disease (GD). We aimed to profile the differentially expressed circRNAs (DEcRs) in plasma exosomes of patients with GD and speculate and probe the functions of the DEcR by comprehensive bioinformatics analyses. Methods: Serum exosomes were isolated from five primary GD patients and five healthy controls via ultracentrifugation. After verification with transmission electron microscopy, exosome samples were subjected to microarray profiling using human circRNA microarrays. Two up-regulated and two down-regulated DEcRs were selected for validation in plasma exosomes from 20 GD and 20 healthy control participants using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The circRNA/microRNA/mRNA interaction network was then assembled and the analysis of the Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways was utilized to predict the potential functions of the DEcR associated genes. Results: There were 15 DEcRs revealed in primary GD cases. The intronic circRNA hsa_circRNA_000102 was confirmed as an up-regulated component in plasma exosomes from patients with GD. The circRNA/microRNA/mRNA interaction network unveiled the most potential targeting microRNAs of hsa_circRNA_000102 and its associated genes. The functional analyses predicted involvement of hsa_circRNA_000102 associated genes in pathways of immune system activation, such as viral infection and interferon-beta signaling. Conclusions: hsa_circRNA_000102 is a differentially up-regulated plasma exosomal circRNA in patients with GD. Our study highlights multiple pathways, particularly virus infection and interferon-beta signaling, for mediating immune activation in Graves' disease.

Different activated carbons and manganese-modified activated carbons were prepared as deoxidizers for the low-concentration coalbed methane deoxidation. The results show that the deoxidation performance of the different activated carbons is affected by raw materials for preparation of deoxidizers. One type of activated carbon exhibited the best performance of oxygen removal, due to the relatively low ash content and better adsorption performance. Experimental results show that the manganese-modified deoxidizers exhibit an excellent deoxidation efficiency at low temperature. At 200 ºC, one kind of manganese-modified activated carbons decreased the oxygen content to less than 1 vol%, which could maintain about 600 min. This indicated that the addition of manganese greatly improved the reactivity of oxygen with activated carbon. Characterization results demonstrated that the physical structure and species types of oxygen of activated carbons, and manganese have important effects on the activity of deoxidizers in different temperature ranges.

In previous studies on learner lexicography, design features of both the content and presentation of learner's dictionaries are the two major research concerns. The quality assessment of learner's dictionaries also covers the two dimensions. Terms used for evaluating them are respectively "usability" or "availability" for the former and "findability" or "accessibility" for the latter. However, the lexicographical construction of "learnability", which takes into account the users' reference and learning needs, remains virtually unexplored either theoretically or practically. Compared to the features of dictionary design mentioned above, "learnability" as the design philosophy of learner lexicography is worth more serious consideration. The present paper aims at exploring the lexicographical notion of "learnability" by way of introducing the neglected legacy of Robert Morrison in his compilation of Wuche Yunfu (1819)1, which is characterized by a high degree of learnability illustrated in the dictionary entries. Morrison's pioneering efforts may help with the conceptual clarification of "learnability" in compiling learner's dictionaries, bilingual ones in particular. Moreover, it is hoped that the recognition of Morrison's lexicographical practice will be beneficial to the future production of better Chinese-English dictionaries for non-native Chinese learners.

In vorige studies van aanleerderleksikografie is ontwerpkenmerke van beide die inhoud en aanbieding van aanleerderswoordeboeke die twee belangrikste navorsingsaspekte. Die kwaliteitsbepaling van aan-leerderswoordeboeke dek ook hierdie twee dimensies. Terme wat gebruik word vir hul evaluering is onderskeidelik "bruikbaarheid" of "beskikbaarheid" vir eersgenoemde en "vindbaarheid" of "toe-ganklikheid" vir laasgenoemde. Die leksikografiese begrip "leerbeerheid", wat die gebruikers se verwysings- en aanleerdersbehoeftes in ag neem, bly egter eintlik teoreties en prakties onontgin. Vergeleke met die kenmerke van woordeboekontwerp waarna hierbo verwys is, behoort "leerbaar-heid" as die ontwerpfilosofie van aanleerdersleksikografie ernstiger oorweeg te word. In hierdie artikel word gepoog om die leksikografiese konsep van "leerbaarheid" te ondersoek met behulp van die vergete nalatenskap van Robert Morrison in sy samestelling van Wuche Yunfu (1819)¹, wat gekenmerk word deur 'n hoë mate van leerbaarheid soos geïllustreer in die woorde-boekinskrywings. Morrison se baanbrekerswerk kan van hulp wees met die konseptuele verheldering van "leerbaarheid" in die samestelling van aanleerderswoordeboeke, veral tweetalige aanleer-derswoordeboeke. Bowenal word daar gehoop dat die erkenning van Morrison se leksikografiese praktyk tot voordeel van die toekomstige produksie van beter Chinees-Engelse woordeboeke vir nie-moedertaal Chinese aanleerders sal wees.

Systemic lupus erythematosus (SLE) is a chronic, autoimmune disorder that affects nearly all organs and tissues. As knowledge about the mechanism of SLE has increased, some immunosuppressive agents have become routinely used in clinical care, and infections have become one of the direct causes of mortality in SLE patients. To identify the risk factors indicative of infection in SLE patients, a case control study of our hospital's medical records between 2011 and 2013 was performed. We reviewed the records of 117 SLE patients with infection and 61 SLE patients without infection. Changes in the levels of T cell subsets, immunoglobulin G (IgG), complement C3, complement C4, globulin, and anti-double-stranded DNA (anti-ds-DNA) were detected. CD4+ and CD4+/CD8+ T cell levels were significantly lower and CD8+ T cell levels were significantly greater in SLE patients with infection than in SLE patients without infection. Additionally, the concentrations of IgG in SLE patients with infection were significantly lower than those in SLE patients without infection. However, complement C3, complement C4, globulin, and anti-ds-DNA levels were not significantly different in SLE patients with and without infection. Therefore, clinical testing for T cell subsets and IgG is potentially useful for identifying the presence of infection in SLE patients and for distinguishing a lupus flare from an acute infection.

Abstract Background: The gamma-type phospholipase A2 inhibitor (ΡLIγ) is a natural protein commonly found in snake serum, which can neutralize pathophysiological effects of snake venom phospholipases A2. Therefore, this protein is a potential candidate to the development of a novel antivenom. To the best of our knowledge, there is no antibody currently available for PLIγ identification and characterization. Methods: Bioinformatics prediction of epitope using DNAStar software was performed based on the sequence of Sinonatrix annularis PLIγ (SaPLIγ). The best epitope 151CPVLRLSNRTHEANRNDLIKVA172 was chosen and synthesized, and then conjugated to keyhole limpet hemocyanin and bovine serum albumin for use as an immunogen and plate-coating antigen, respectively. Results: Eighteen IgG anti-PLIγ mAb hybridoma cell strains were obtained, and all the mAbs had positive interaction with recombinant His6-PLIγ and natural SaPLIγ. Moreover, the mAb from 10E9 strain was also successfully used for the immunodetection of other snake serum PLIγs. cDNA sequence alignment of those PLIγs from different snake species showed that their epitope segments were highly homologous. Conclusions: The successful preparation of anti-PLIγmAb is significant for further investigation on the relationship between the structure and function of PLIγs, as well as the interaction between PLIγs and PLA2s.